CN211042749U - ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels - Google Patents
ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels Download PDFInfo
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- CN211042749U CN211042749U CN201922039809.6U CN201922039809U CN211042749U CN 211042749 U CN211042749 U CN 211042749U CN 201922039809 U CN201922039809 U CN 201922039809U CN 211042749 U CN211042749 U CN 211042749U
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Abstract
The utility model relates to an ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels, wherein an extrusion fixing cabin and a driving oil cylinder are respectively arranged on the ROV, the ROV provides hydraulic oil for the driving oil cylinder, the front end and the rear end of the extrusion fixing cabin are respectively connected with a front cover and a rear cover in a sealing and threaded manner, a rigid body is inserted into the extrusion fixing cabin and is connected with a piston, and the piston is in sealing and sliding connection with the inner wall of the extrusion fixing cabin; an extrusion front plate is arranged on the inner surface of the front cover, an extrusion rear plate is arranged on the surface of one side, facing the front cover, of the piston, and a throwing port for throwing the biological sample into the cabin is formed in the extrusion fixing cabin; the fixed liquid storage bag is internally stored with fixed solution and is communicated with the inside of the extrusion fixing cabin between the extrusion front plate and the extrusion rear plate through a rubber tube channel. The utility model discloses satisfy the fixed demand of macrobiosis normal position such as mussel of deep sea ecosystem, it is little influenced by the sample depth, and corrosion resisting property is strong, deposits bulky, and the flexible operation is stable to can fix macrobiosis sample fast, effectively.
Description
Technical Field
The utility model belongs to the fixed field of macrobiotic normal position such as deep sea mussel, specifically speaking are macrobiotic extrusion formula normal position fixing device such as deep sea mussel based on ROV.
Background
Because the deep sea environment and the upper ocean environment have huge physicochemical difference, and the sampling is difficult to be completed in a short time by the water depth of thousands of meters, the physiological state of organisms in the sampling process is undoubtedly changed greatly. The current sampling means cannot truly reproduce the real state of deep sea, and is a main bottleneck for restricting the research of deep sea organisms. Deep-sea organisms adaptively evolve under extreme environments to generate a series of special environmental stress coping mechanisms of natural products, and gene resources which need to be developed urgently are stored.
At present, research and development of related fixed equipment are carried out by a plurality of domestic marine research institutions; however, most of the studies on the immobilization technology of deep-sea organisms are mainly conducted by simple storage mechanisms for microorganisms or large-sized organisms, such as: a microorganism in-situ automatic enrichment fixing device, a deep sea extreme environment microorganism primary productivity in-situ detection device and the like. The fixing equipment for large-scale organisms is still in a preliminary research stage, no or few examples of research and application are provided in China, and the difficulty is large-scale organism capturing mode, crushing mode, fixing mode and the like. Moreover, the overall structure of the simple storage mechanism for microorganisms or large-sized organisms is not suitable for ROV (remote operated vehicle) operation, and the biological fixing effect is not good.
SUMMERY OF THE UTILITY MODEL
Refer to the fixed advantage of traditional deep sea microorganism normal position enrichment, combine the characteristics of large-scale living beings normal position fixing modes such as deep sea mussel, the utility model aims to provide a large-scale living beings extrusion formula normal position fixing device such as deep sea mussel based on ROV. The utility model discloses utilize ROV's accurate location capture ability, power supply ability, communication ability, make implementation's degree of difficulty greatly reduced.
The purpose of the utility model is realized through the following technical scheme:
the utility model discloses a bag, protecgulum, extrusion fixed cabin, back lid, actuating cylinder, extrusion front bezel, extrusion back plate, rigid body and piston are deposited to the stationary liquid, wherein extrude fixed cabin and actuating cylinder and install respectively on the ROV, and this ROV provides hydraulic oil for actuating cylinder, the both ends are sealed threaded connection respectively around the extrusion fixed cabin have protecgulum and back lid, and the rigid body that is regarded as the actuating cylinder output inserts in the extrusion fixed cabin, and is connected with the piston, this piston with the sealed sliding connection of extrusion fixed cabin inner wall; an extrusion front plate is arranged on the inner surface of the front cover, an extrusion rear plate linked with the piston is arranged on the surface of one side, facing the front cover, of the piston, and a throwing port for throwing the biological sample into the cabin is formed in the extrusion fixing cabin; the fixed liquid storage bag is internally provided with a fixed solution and is communicated with the interior of the extrusion fixed cabin between the extrusion front plate and the extrusion rear plate through a rubber tube channel.
Wherein: the extrusion surfaces of the extrusion front plate and the extrusion rear plate are inclined planes.
The inclined planes of the extrusion front plate and the extrusion rear plate are parallel.
The driving oil cylinder is provided with a guide disc, at least one guide hole is formed in the guide disc, a guide rod penetrates through the guide hole, one end of the guide rod is inserted into the extrusion fixing cabin and is connected with the piston to limit the rotation of the piston and the driving oil cylinder, and the other end of the guide rod always slides in the guide hole.
The front cover is provided with a pressure relief joint, one end of the pressure relief joint is communicated with the inside of the extrusion fixing cabin between the extrusion front plate and the extrusion rear plate, the other end of the pressure relief joint is communicated with the outside through a pipeline, and the pipeline is provided with a pressure relief valve.
The front cover is provided with a fixed joint, one end of the fixed joint is communicated with the inside of an extrusion fixing cabin between the extrusion front plate and the extrusion rear plate, the other end of the fixed joint is communicated with a fixed liquid storage bag through the rubber pipe channel, and the rubber pipe channel is provided with a fixed valve.
And a horn mouth convenient for putting the biological sample is arranged on the outer edge of the putting opening.
The length of the driving oil cylinder is equal to the moving distance of the rigid body in the extrusion fixing cabin.
The utility model discloses an advantage does with positive effect:
1. the utility model discloses satisfy the fixed demand of macrobiosis normal position such as mussel of deep sea ecosystem, it is little influenced by the sample depth, and corrosion resisting property is strong, deposits bulky, and the flexible operation is stable to can fix macrobiosis sample fast, effectively.
2. The utility model discloses based on the ROV system, characteristics such as accurate positioning, real-time communication, effective controllable that can reuse ROV.
3. The utility model discloses the small and exquisite compactness of structure, the steady operation error is little to applicable complicated submarine environment under multiple degree of depth, temperature and ocean current environment, but wide application acquires, fixes in macrobiology such as mussel that need strict fidelity.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention;
wherein: the device comprises a fixed liquid storage bag 1, a rubber tube channel 2, a fixed valve 3, a fixed joint 4, a pressure relief valve 5, a pressure relief joint 6, a front cover 7, an extrusion fixed cabin 8, a horn mouth 9, a placing opening 10, a rear cover 11, a guide rod 12, a bolt 13, a guide hole 14, a driving oil cylinder 15, an oil port 16, an extrusion front plate 17, an extrusion rear plate 18, a rigid body 19, a guide disc 20, a piston 21 and a sealing O ring 22.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings.
As shown in fig. 1, the utility model discloses a bag is deposited to stationary liquid 1, protecgulum 7, extrusion fixed cabin 8, back lid 11, actuating cylinder 15, extrusion front bezel 17, extrusion back plate 18, rigid body 19 and piston 21, wherein extrude fixed cabin 8 and actuating cylinder 15 and utilize fixed fastener and ROV bottom plate to carry out fixed mounting respectively, ROV utilizes hydraulic fluid port 16 on the actuating cylinder 15 to provide hydraulic oil for actuating cylinder 15 to accomplish rigid body 19's round trip movement, stationary solution has been deposited in the bag is deposited to stationary liquid 1, the stationary solution of this embodiment is stationary liquid RNA L ater.
The front end and the rear end of the extrusion fixing cabin 8 are respectively connected with a front cover 7 and a rear cover 11 in a sealing threaded manner, a rigid body 19 (namely a piston rod of the driving oil cylinder 15) output by the driving oil cylinder 15 is inserted into the extrusion fixing cabin 8 through the rear cover 11 and is connected with a piston 21, a sealing O ring 22 is arranged on the outer ring of the piston 21, and the piston 21 is in sealing sliding connection with the inner wall of the extrusion fixing cabin 8; in the embodiment, a rear extrusion plate 18 is fixedly connected to one side surface of the piston 21 facing the front cover 7, and the other side surface of the piston 21 facing the rear cover 11 is fixedly connected to the rigid body 19 through two bolts 13; an extrusion front plate 17 is fixedly connected with the inner surface of the front cover 7. In this embodiment, the pressing surfaces of the pressing front plate 17 and the pressing rear plate 18 are both inclined surfaces (inclined angle is 60 °), and the inclined surfaces of the pressing front plate 17 and the pressing rear plate 18 are parallel to each other, thereby pressing a large biological sample. The length of the driving oil cylinder 15 is equal to the moving distance of the rigid body 19 in the extrusion fixing chamber 8, and the driving oil cylinder is used for ensuring that the extrusion crushing distance of the biological sample in the extrusion fixing chamber 8 and the volume of the extracted fixing solution are realized in a set mode.
During the reciprocating motion of the rigid body 19 under the independent operation of the driving cylinder 15, the piston 21 is easy to rotate. In order to avoid the rotation of the piston 21 and the driving cylinder 15, a guide disc 20 is installed on the driving cylinder 15, at least one guide hole 14 (two guide holes 14 in this embodiment) is opened on the guide disc 20, a guide rod 12 penetrates through the guide hole 14, one end of the guide rod 12 is inserted into the extrusion fixing cabin 8 through the rear cover 11 and is connected with the piston 13 to limit the rotation of the piston 13 and the driving cylinder 15, and the other end of the guide rod 12 freely slides in the guide hole 14 all the time.
The extrusion fixing cabin 8 is provided with a throwing port 10 for throwing the biological samples into the cabin, and the opening diameter of the throwing port 10 can be adjusted according to the external dimensions of large organisms such as deep sea mussels and the like; in order to put the biological sample into the putting-in opening 10, the bell mouth 9 for putting the biological sample is arranged at the outer edge of the putting-in opening 10.
The front cover 7 is respectively provided with a fixed joint 4 and a pressure relief joint 6, one end of the fixed joint 4 is communicated with the inside of an extrusion fixed cabin 8 between an extrusion front plate 17 and an extrusion rear plate 18, the other end is communicated with a fixed liquid storage bag 1 through a rubber tube channel 2, and the rubber tube channel 2 is provided with a fixed valve 3. One end of the pressure relief joint 6 is communicated with the inside of the extrusion fixing cabin 8 between the extrusion front plate 17 and the extrusion rear plate 18, the other end is communicated with the outside through a pipeline, and a pressure relief valve 5 is arranged on the pipeline. The fixed liquid storage bag 1, the rubber tube channel 2, the fixed valve 3, the fixed joint 4 and the front cover 7 form a passage.
The hose passage 2 of the present embodiment is manufactured by saint-gobain, france. The utility model discloses an extrusion formula normal position fixing device wholly uses non-metallic transparent material (like special fluorine dragon), and fixed valve 3, pressure relief valve 5, horn mouth 9 adopt metal material (Ti alloy) processing to accomplish, avoid the pollution of sample.
The utility model discloses an extrusion formula normal position fixing device needs to acquire, broken, fixed method to macrobiology such as deep sea mussel, including following step:
firstly, integrally disassembling and cleaning a shore-based end, integrally disassembling and disassembling large-scale organisms in-situ fixing devices such as deep sea mussels and the like, and then cleaning;
step two, assembling after cleaning, wherein the fixed liquid storage bag 1 is filled with a fixed solution in an initial state, the piston 21 is abutted against the rear cover 11, and the fixed valve 3 on the rubber tube channel 2 between the inside of the extrusion fixing cabin 8 and the fixed liquid storage bag 1 and the pressure relief valve 5 on the pipeline communicated with the outside inside of the extrusion fixing cabin 8 are both in a closed state;
thirdly, observing and grabbing large biological samples (such as mussels) through ROV deep sea high-definition camera shooting, putting the large biological samples into a bell mouth 9, enabling the biological samples to fall into an extrusion fixed cabin 8 through the action of gravity, opening a pressure relief valve 5, starting a driving oil cylinder 15, enabling a rigid body 19 to stretch out and move, driving an extrusion rear plate 18 to stretch forward through a piston 21, and enabling the extrusion rear plate 18 to push the large biological samples to be in contact with an extrusion front plate 17 to finish the extrusion process; discharging water in the extrusion fixing cabin 8 through a pressure relief valve 5, closing the pressure relief valve 5 by using an ROV manipulator, opening a fixing valve 3, starting a driving oil cylinder 15 in the opposite direction to enable a rigid body 19 to contract and move, driving an extrusion rear plate 18 to retreat through a piston 21, enabling the interior of the extrusion fixing cabin 8 to form suction, sucking a fixing solution in a fixing solution storage bag 1, and finishing the fixing work of a biological sample; in the contraction movement of the rigid body 19, ROV deep sea high definition camera shooting is used for observing the movement condition of the piston 21, so that a space is reserved between the piston 21 and the front end of the throwing port 10 (namely the rear edge of the piston 33 does not contact the front end of the throwing port 10), and in the whole movement process, a sealing O ring 22 is used for ensuring the tightness of the extrusion fixing cabin 8;
step four, sampling at the shore base end; and (4) recovering the in-situ fixing device to the shore base end, dismantling the front cover 7, and taking out large organisms such as mussels to complete the whole operation.
The utility model discloses the small and exquisite compactness of structure, corrosion resisting property is strong, and it is little to rotate smooth operation error to applicable complicated submarine environment under multiple degree of depth, temperature and ocean current environment, but wide application acquires, fixes in macrobiology such as mussel that needs strict fidelity. The utility model discloses satisfy macrobiosis's fixed demand, can use under the complicated variable environment of deep sea pressure, will provide the support for the biochemical research of various physiology of deep sea macrobiosis.
Claims (8)
1. The utility model provides a deep sea mussel etc. macrobiosis extrusion formula normal position fixing device based on ROV which characterized in that: the device comprises a fixed liquid storage bag (1), a front cover (7), an extrusion fixing cabin (8), a rear cover (11), a driving oil cylinder (15), an extrusion front plate (17), an extrusion rear plate (18), a rigid body (19) and a piston (21), wherein the extrusion fixing cabin (8) and the driving oil cylinder (15) are respectively installed on an ROV (remote operated vehicle), the ROV provides hydraulic oil for the driving oil cylinder (15), the front end and the rear end of the extrusion fixing cabin (8) are respectively connected with the front cover (7) and the rear cover (11) in a sealing threaded manner, the rigid body (19) output by the driving oil cylinder (15) is inserted into the extrusion fixing cabin (8) and is connected with the piston (21), and the piston (21) is connected with the inner wall of the extrusion fixing cabin (8) in a sealing sliding manner; an extrusion front plate (17) is arranged on the inner surface of the front cover (7), an extrusion rear plate (18) linked with the piston (21) is arranged on the surface of one side, facing the front cover (7), of the piston (21), and a throwing port (10) for throwing the biological sample into the cabin is formed in the extrusion fixing cabin (8); the fixed solution is stored in the fixed solution storage bag (1), and the fixed solution storage bag (1) is communicated with the interior of the extrusion fixed cabin (8) between the extrusion front plate (17) and the extrusion rear plate (18) through the rubber tube channel (2).
2. The ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels is characterized in that: the extrusion surfaces of the extrusion front plate (17) and the extrusion rear plate (18) are inclined surfaces.
3. The ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels is characterized in that: the inclined surfaces of the extrusion front plate (17) and the extrusion rear plate (18) are parallel.
4. The ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels is characterized in that: install guiding disc (20) on actuating cylinder (15), seted up at least one guiding hole (14) on this guiding disc (20), wear to be equipped with guide bar (12) in guiding hole (14), the one end of this guide bar (12) is inserted in the fixed cabin of extrusion (8), and with piston (13) link to each other, restriction piston (13) and actuating cylinder (15) rotate, and the other end of guide bar (12) is in all the time slide in guiding hole (14).
5. The ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels is characterized in that: the pressure relief joint (6) is installed on the front cover (7), one end of the pressure relief joint (6) is communicated with the inside of the extrusion fixing cabin (8) between the extrusion front plate (17) and the extrusion rear plate (18), the other end of the pressure relief joint is communicated with the outside through a pipeline, and a pressure relief valve (5) is installed on the pipeline.
6. The ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels is characterized in that: the front cover (7) is provided with a fixed joint (4), one end of the fixed joint (4) is communicated with the inside of an extrusion fixed cabin (8) between the extrusion front plate (17) and the extrusion rear plate (18), the other end of the fixed joint is communicated with the fixed liquid storage bag (1) through the rubber pipe channel (2), and the rubber pipe channel (2) is provided with a fixed valve (3).
7. The ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels is characterized in that: and a bell mouth (9) convenient for throwing the biological sample is arranged on the outer edge of the throwing port (10).
8. The ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels is characterized in that: the length of the driving oil cylinder (15) is equal to the moving distance of the rigid body (19) in the extrusion fixed cabin (8).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922039809.6U CN211042749U (en) | 2019-11-22 | 2019-11-22 | ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201922039809.6U CN211042749U (en) | 2019-11-22 | 2019-11-22 | ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels |
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| CN211042749U true CN211042749U (en) | 2020-07-17 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114431197A (en) * | 2022-01-20 | 2022-05-06 | 南方海洋科学与工程广东省实验室(广州) | Marine benthos in-situ fixing system and method |
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- 2019-11-22 CN CN201922039809.6U patent/CN211042749U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114431197A (en) * | 2022-01-20 | 2022-05-06 | 南方海洋科学与工程广东省实验室(广州) | Marine benthos in-situ fixing system and method |
| CN114431197B (en) * | 2022-01-20 | 2022-11-04 | 南方海洋科学与工程广东省实验室(广州) | Marine benthos in-situ fixing system and method |
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