CN211235013U

CN211235013U - ROV-based cutting type in-situ fixing device for large organisms such as deep sea mussels

Info

Publication number: CN211235013U
Application number: CN201922039601.4U
Authority: CN
Inventors: 连超; 李超伦; 王敏晓; 栾振东; 张鑫; 陈浩; 王昊; 杜增丰; 陈杨; 丛石磊; 马文肖
Original assignee: Institute of Oceanology of CAS
Current assignee: Institute of Oceanology of CAS
Priority date: 2019-11-22
Filing date: 2019-11-22
Publication date: 2020-08-11
Anticipated expiration: 2029-11-22

Abstract

The utility model relates to a cutting type in-situ fixing device for large organisms such as deep sea mussels based on ROV, wherein a fixing cabin and a driving oil cylinder are respectively arranged on the ROV, the ROV provides hydraulic oil for the driving oil cylinder, one end of the fixing cabin is hermetically connected with a front cover, the other end of the fixing cabin is hermetically connected with one end of a storage cabin, the other end of the storage cabin is hermetically connected with a rear cover, a knife rest with a cutting knife is arranged in the fixing cabin, and a throwing opening is arranged on the storage cabin; the rigid body passes through the rear cover and then is inserted into the storage cabin and is connected with one side of the piston, the other side of the piston is connected with a cutting knife pressing plate, and the piston is in sealed sliding connection with the inner wall of the storage cabin; the fixed solution is stored in the fixed solution storage bag, and the fixed solution storage bag is communicated with the inside of the fixed cabin through a rubber tube channel. The utility model discloses satisfy the fixed demand of macrobiosis normal position such as mussel of deep sea ecosystem, it is little influenced by the sample depth, and corrosion resisting property is strong, deposits bulky, and the flexible operation is stable to can fix macrobiosis sample fast, effectively.

Description

ROV-based cutting type in-situ fixing device for large organisms such as deep sea mussels

Technical Field

The utility model belongs to the fixed field of macrobiosis normal position such as deep sea mussel, specifically speaking are macrobiosis cutting formula normal position fixing device such as deep sea mussel based on ROV.

Background

Because the deep sea environment and the upper ocean environment have huge physicochemical difference, and the sampling is difficult to be completed in a short time by the water depth of thousands of meters, the physiological state of organisms in the sampling process is undoubtedly changed greatly. The current sampling means cannot truly reproduce the real state of deep sea, and is a main bottleneck for restricting the research of deep sea organisms. Deep-sea organisms adaptively evolve under extreme environments to generate a series of special environmental stress coping mechanisms of natural products, and gene resources which need to be developed urgently are stored.

At present, research and development of related fixed equipment are carried out by a plurality of domestic marine research institutions; however, most of the studies on the immobilization technology of deep-sea organisms are mainly conducted by simple storage mechanisms for microorganisms or large-sized organisms, such as: a microorganism in-situ automatic enrichment fixing device, a deep sea extreme environment microorganism primary productivity in-situ detection device and the like. The fixing equipment for large-scale organisms is still in a preliminary research stage, no or few examples of research and application are provided in China, and the difficulty is large-scale organism capturing mode, crushing mode, fixing mode and the like. Moreover, the overall structure of the simple storage mechanism for microorganisms or large-sized organisms is not suitable for ROV (remote operated vehicle) operation, and the biological fixing effect is not good.

SUMMERY OF THE UTILITY MODEL

Refer to the fixed advantage of traditional deep sea microorganism normal position enrichment, combine the characteristics of the fixed mode of macrobiosis normal position such as deep sea mussel, the utility model aims to provide a cutting formula normal position fixing device of macrobiosis such as deep sea mussel based on ROV. The utility model discloses utilize ROV's accurate location capture ability, power supply ability, communication ability, make implementation's degree of difficulty greatly reduced.

The purpose of the utility model is realized through the following technical scheme:

the utility model discloses a bag, protecgulum, stationary bin, storage compartment, back lid, knife rest, cutting knife clamp plate, actuating cylinder, rigid body, piston and cutting knife are deposited to the stationary bin and actuating cylinder install respectively on the ROV, and this ROV provides hydraulic oil for actuating cylinder, the one end and the protecgulum sealing connection of stationary bin, the other end and the one end sealing connection of storage compartment, the other end sealing connection of this storage compartment has the back lid, install the knife rest of taking the cutting knife in the stationary bin, set up the input mouth of putting in the biological sample on the storage compartment; a rigid body output by the driving oil cylinder penetrates through the rear cover and then is inserted into the storage cabin and is connected with one side of a piston, the other side of the piston is connected with a cutting knife pressing plate linked with the piston, and the piston is in sealed sliding connection with the inner wall of the storage cabin; the fixed solution is stored in the fixed solution storage bag, and the fixed solution storage bag is communicated with the inside of the fixed cabin through a rubber tube channel.

Wherein: one end of the knife rest is of an annular structure and is arranged on the inner surface of the other end of the fixed cabin, two extending parts extending axially are symmetrically arranged on the end face of the annular structure facing the front cover, each extending part is of a hollow structure, the edge of one hollow side is the end face of the annular structure facing the front cover, a plurality of grooves are formed in the edge of the other hollow side, and a cutting knife is arranged in each groove.

The width of the extension parts gradually reduces towards the middle along the pushing direction of the biological sample, and a cambered surface bending towards one side of the piston is arranged between the two symmetrical extension parts.

The edge of the other side of the hollow part is in a V shape; the grooves on the extending parts on the two sides are equal in number and correspond to one another, and the two ends of each cutting knife are respectively installed in the two corresponding grooves.

The cutting knife pressing plate is formed by stacking a plurality of pressing plates, each pressing plate is equally divided into a pressing plate guide plate and a pressing plate extrusion part, one side of each pressing plate extrusion part is connected with the other side of the piston, a cutting knife pressing plate notch with a V-shaped horizontal projection is arranged on the other side of each pressing plate extrusion part, pressing plate guide plates are symmetrically arranged on two sides of the cutting knife pressing plate notch, and the pressing plate guide plates on each pressing plate penetrate through the space between two adjacent cutting knives in the moving process along with the piston.

The driving oil cylinder is provided with a guide disc, at least one guide hole is formed in the guide disc, a guide rod penetrates through the guide hole, one end of the guide rod is inserted into the storage cabin and is connected with the piston to limit the rotation of the piston and the driving oil cylinder, and the other end of the guide rod always slides in the guide hole.

The front cover is respectively provided with a fixed joint, a pressure relief joint A and a pressure relief joint B, a fixed liquid extension pipe is arranged in the fixed cabin and is communicated with one end of the fixed joint, the other end of the fixed joint is communicated with a fixed liquid storage bag through a rubber pipe channel, and a fixed valve is arranged on the rubber pipe channel; one end of the pressure relief joint A is in threaded connection with the front cover and is communicated with the inner side of the fixed cabin, the other end of the pressure relief joint A is communicated with the outside through a pipeline, and a pressure relief valve is arranged on the pipeline; one end of the pressure relief joint B is in threaded connection with the front cover and communicated with the inner side of the fixed cabin, the other end of the pressure relief joint B is communicated with the outside through a pipeline, and a one-way valve is arranged on the pipeline.

The length of the driving oil cylinder is equal to the moving distance of the rigid bodies in the storage cabin and the fixed cabin.

The utility model discloses an advantage does with positive effect:

1. the utility model discloses satisfy the fixed demand of macrobiosis normal position such as mussel of deep sea ecosystem, it is little influenced by the sample depth, and corrosion resisting property is strong, deposits bulky, and the flexible operation is stable to can fix macrobiosis sample fast, effectively.

2. The utility model discloses based on the ROV system, characteristics such as accurate positioning, real-time communication, effective controllable that can reuse ROV.

3. The utility model discloses the small and exquisite compactness of structure, the steady operation error is little to applicable complicated submarine environment under multiple degree of depth, temperature and ocean current environment, but wide application acquires, fixes in macrobiology such as mussel that need strict fidelity.

4. The utility model discloses a form a rhombus between cutting knife and the cutting knife clamp plate, can become the shellfish by horizontal vertical, cutting effect is good.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a top view of the structure of the present invention with the stationary liquid storage bag and the hose passage removed;

fig. 3 is a schematic perspective view of the tool holder of the present invention;

FIG. 4 is a front view of the structure of the tool holder of the present invention;

FIG. 5 is a top view of the structure of FIG. 4 with the cutting knife installed;

FIG. 6 is a side view of the structure of the utility model after the cutter frame is provided with the cutter;

fig. 7 is a front view of the structure of the cutting knife pressing plate of the present invention;

FIG. 8 is a top view of FIG. 7;

wherein: the device comprises a fixed liquid storage bag 1, a rubber tube channel 2, a fixed valve 3, a fixed joint 4, a pressure relief valve 5, a pressure relief joint A6, a front cover 7, a fixed cabin 8, a horn mouth 9, a feeding port 10, a storage cabin 11, a piston sealing ring 12, a rear cover 13, a knife rest 14, an annular structure 1401, an extension 1402, a cutter pressing plate 15, a pressing plate guide plate 1501, a pressing plate extrusion part 1502, a pressing plate extrusion part 16, a guide rod 17, a bolt 18, a guide hole 18, a driving oil cylinder 19, an oil port 20, a cutter pressing plate notch 21, a small fixed clamping piece 22, a large fixed clamping piece 23, a rigid body 24, a guide disc 25, a piston 26, a cutter 27, a sealing O ring 28, a check valve 29, a pressure relief joint B30, a fixed liquid extension pipe 31 and a groove 32.

Detailed Description

The present invention will be described in detail with reference to the accompanying drawings.

As shown in fig. 1-8, the utility model discloses a bag is deposited to stationary liquid 1, protecgulum 7, stationary chamber 8, deposit cabin 11, back lid 13, knife rest 14, cutting knife clamp plate 15, actuating cylinder 19, rigid body 24, piston 26 and cutting knife 27, wherein actuating cylinder 19 utilizes two fixed little fastener 22 and fixed mounting on the ROV bottom plate, and the ROV utilizes hydraulic fluid port 20 on actuating cylinder 19 to provide hydraulic oil for actuating cylinder 19 to accomplish the round trip action of rigid body 24; the fixed cabin 8 is fixedly arranged on the ROV bottom plate by using a large fixed clamping piece 23, one end of the fixed cabin 8 is hermetically connected with the front cover 7, the other end of the fixed cabin 8 is hermetically connected with one end of the storage cabin 11, and the other end of the storage cabin 11 is hermetically connected with the rear cover 13. A fixative solution is stored in the fixative solution storing bag 1, and the fixative solution in this embodiment is a fixative solution RNAlater.

A knife rest 14 with a cutting knife 27 is arranged in the fixed cabin 8, a throwing opening 10 for throwing the biological sample into the cabin is arranged on the storage cabin 11, and the opening diameter of the throwing opening 10 can be adjusted according to the external dimension of large organisms such as deep sea mussels and the like; in order to put the biological sample into the putting-in opening 10, the bell mouth 9 for putting the biological sample is arranged at the outer edge of the putting-in opening 10. A rigid body 24 (i.e. a piston rod of the driving cylinder 19) as an output of the driving cylinder 19 passes through the rear cover 13 and then is inserted into the storage compartment 11, and is fixedly connected with one side of a piston 26 through two bolts 17, and a cutting knife pressing plate 15 which is linked with the piston 26 is welded on the other side of the piston 26. A sealing O-ring 28 is mounted on the outer ring of the piston 26, and the piston 26 is sealingly and slidably connected to the inner wall of the storage compartment 11. The length of the driving oil cylinder 19 of the embodiment is equal to the moving distance of the rigid body 24 in the storage cabin 11 and the fixed cabin 8, and the driving oil cylinder is used for ensuring that the crushing distance of the biological sample in the fixed cabin 8 and the volume of the extracted fixed liquid are realized in a set mode.

During the reciprocating motion of the rigid body 24 with the independent operation of the driving cylinder 19, the piston 26 is likely to rotate. In order to avoid the rotation of the piston 26 and the driving cylinder 19, a guide disc 25 is mounted on the driving cylinder 19, at least one guide hole 18 (two guide holes 18 in this embodiment) is formed in the guide disc 25, a guide rod 16 penetrates through the guide hole 18, one end of the guide rod 16 is inserted into the storage compartment 11 through the rear cover 13 and is connected with the piston 26 to limit the rotation of the piston 26 and the driving cylinder 19, and the other end of the guide rod 16 always freely slides in the guide hole 18.

The front cover 7 is respectively provided with a fixed joint 4, a pressure relief joint A6 and a pressure relief joint B30, a fixed liquid extension pipe 31 is arranged in the fixed cabin 8, the fixed liquid extension pipe 31 is communicated with one end of the fixed joint 4, the other end of the fixed joint 4 is communicated with a fixed liquid storage bag 1 through a rubber pipe channel 2, and a fixed valve 3 is arranged on the rubber pipe channel 2. One end of the pressure relief joint A6 is in threaded connection with the front cover 7 and communicated with the inside of the fixed cabin 8, the other end of the pressure relief joint A6 is communicated with the outside through a pipeline, and a pressure relief valve 5 is arranged on the pipeline. One end of the pressure relief joint B30 is in threaded connection with the front cover 7 and communicated with the inside of the fixed cabin 8, the other end of the pressure relief joint B30 is communicated with the outside through a pipeline, and a one-way valve 29 is arranged on the pipeline. The stationary liquid storage bag 1, the rubber tube channel 2, the stationary valve 3, the stationary joint 4, the front cover 7 and the stationary liquid extension tube 31 form a passage.

One end of the tool holder 14 is an annular structure 1401 (in this embodiment, a circular ring) and is installed on the inner surface of the other end of the fixed cabin 8, two extending portions 1402 extending in the axial direction are symmetrically arranged on the end surface of the annular structure 1401 facing the front cover 7, each extending portion 1402 is a hollow structure, the edge of one hollow side is the end surface of the annular structure 1401 facing the front cover 7, a plurality of grooves 32 are formed in the edge of the other hollow side, and a cutting knife 27 is installed in each groove 32. The width of the extending portions 1402 gradually decreases toward the middle along the biological sample pushing direction, and a curved surface curved toward the piston 26 side is formed between two symmetrical extending portions 1402. The other side edge of the hollow part is in a V shape, so that the horizontal projection of the cutting knife 27 arranged between the two extending parts 1402 is in a V shape. The grooves 32 on the two side extension portions 1402 are equal in number and correspond to each other one by one, and two ends of each cutting knife 27 are respectively installed in the two corresponding grooves 32. The horizontal projection of the extension 1402 of this embodiment is an isosceles trapezoid, the middle hollow structure is an isosceles triangle, two waists of the isosceles triangle are the edges of the other side of the isosceles triangle, the same number of grooves 32 are formed on each waist, and the grooves 32 are formed along the pushing direction of the biological sample. The vertical projection of the portion between the two extensions 1402 is in the shape of a "C". The inner surface of the tool rest 14 is provided with a piston sealing ring 12, the outer surface of the piston 26 is sleeved with a sealing O-ring 28, when the driving oil cylinder 19 finishes the forward movement, the sealing O-ring 28 on the piston 26 reaches the position of the piston sealing ring 12, and the fixed cabin 8 becomes a closed cabin body through the action of the sealing O-ring 28 and the piston sealing ring 12.

The cutting knife pressing plate 15 is formed by stacking a plurality of pressing plates, each pressing plate is divided into a pressing plate guide plate 1501 and a pressing plate extrusion part 1502, one side of each pressing plate extrusion part 1502 is connected with the other side of the piston 26, a cutting knife pressing plate notch 21 with a V-shaped horizontal projection is arranged on the other side of each pressing plate extrusion part 1502, the pressing plate guide plates 1501 are symmetrically arranged on two sides of the cutting knife pressing plate notch 21, and the pressing plate guide plates 1501 on each pressing plate penetrate through the space between every two adjacent cutting knives 27 in the moving process along with the piston 26. The size of the cutting knife pressing plate notch 21 is designed according to the size of the putting-in opening 10. The cutting knife pressing plate 15 of the embodiment is composed of twelve pressing plates, thirteen cutting knives 27 are provided, and the front end of the pressing plate guide plate 1501 is placed between two adjacent cutting knives 27 in advance for guiding in advance; the V-shape of the cutting blade 27 attached between the V-shaped blade holder notch 21 and the two extending portions 1402 forms a rhombus, and the shellfish can be cut while standing up by changing the horizontal state to the vertical state.

The hose passage 2 of the present embodiment is manufactured by saint-gobain, france. The utility model discloses wholly use non-metallic transparent material (like teflon), fixed valve 3, pressure relief valve 5, horn mouth 9 adopt metal material (Ti alloy) processing to accomplish, avoid the pollution of sample.

The utility model discloses a cutting formula normal position fixing device needs to acquire, broken, fixed method to macrobiology such as deep sea mussel, including following step:

firstly, integrally disassembling and cleaning a shore-based end, integrally disassembling and disassembling large-scale organisms in-situ fixing devices such as deep sea mussels and the like, and then cleaning;

step two, assembling after cleaning, wherein the fixed solution storage bag 1 is filled with the fixed solution in an initial state, the piston 26 is abutted against the rear cover 13, and the fixed valve 3 arranged on the rubber tube channel 2 between the inside of the fixed cabin 8 and the fixed solution storage bag 1 and the pressure relief valve 5 arranged on a communication pipeline between the inside of the fixed cabin 8 and the outside are both in a closed state;

thirdly, observing and grabbing a large biological sample (such as mussel) through ROV deep sea high-definition camera shooting, putting the large biological sample into a bell mouth 9, enabling the biological sample to fall into a fixed cabin 8 through a putting opening 10 under the action of gravity, opening a pressure relief valve 5, starting a driving oil cylinder 19 to enable a rigid body 24 to extend out and move, driving a cutting knife pressing plate 15 to extend forwards through a piston 21, enabling the forward side of the cutting knife pressing plate 15 to contact with the biological sample and driving the biological sample to move forwards, and cutting the biological sample when the biological sample contacts with a cutting knife 27; the size of the biological sample can be adjusted according to the requirement, and the purpose can be realized by adjusting the number and the spacing of the cutting knives 27; after the cutting of the biological sample is completed, the biological sample is pushed into the fixed cabin 8 by the pressing plate squeezing part 1502 of the cutting knife pressing plate 15 through the gap between the cutting knives 27; the water body in the fixed cabin 8 is discharged out of the fixed cabin 8 through the pressure relief valve 5, after the movement of the driving oil cylinder 19 is completed, the sealing O ring 28 on the piston 26 reaches the position of the piston sealing ring 12, the fixed cabin 8 becomes a closed cabin body through the action of the sealing O ring 28 and the piston sealing ring 12, at the moment, all mussels are pushed into the fixed cabin 8, and the pressure relief valve 5 is closed by using an ROV manipulator; opening the fixed valve 3, extruding the fixed liquid storage bag 1 by using an ROV manipulator, so that the fixed liquid enters the fixed cabin 8 through the rubber tube channel 2, the fixed valve 3 and the fixed liquid extension tube 31, and is mixed with the biological sample cut in the fixed cabin 8 to finish the fixation of the biological sample;

step four, sampling at the shore base end; and (3) recovering the in-situ fixing device to the shore base end, wherein in the recovery process, the water depth gradually becomes shallow, the internal pressure of the fixed cabin 8 is gradually greater than the external pressure, and the redundant water body of the fixed cabin 8 is discharged out of the cabin through the one-way valve 37. The front cover 7 is removed from the shore base end, and large organisms such as mussels are taken out to complete the whole operation.

The utility model discloses the small and exquisite compactness of structure, corrosion resisting property is strong, and it is little to rotate smooth operation error to applicable complicated submarine environment under multiple degree of depth, temperature and ocean current environment, but wide application acquires, fixes in macrobiology such as mussel that needs strict fidelity. The utility model discloses satisfy macrobiosis's fixed demand, can use under the complicated variable environment of deep sea pressure, will provide the support for the biochemical research of various physiology of deep sea macrobiosis.

Claims

1. The utility model provides a large-scale living beings cutting formula normal position fixing device such as deep sea mussel based on ROV which characterized in that: the device comprises a fixed liquid storage bag (1), a front cover (7), a fixed cabin (8), a storage cabin (11), a rear cover (13), a knife rest (14), a cutting knife pressing plate (15), a driving oil cylinder (19), a rigid body (24), a piston (26) and a cutting knife (27), wherein the fixed cabin (8) and the driving oil cylinder (19) are respectively installed on an ROV (remote operated vehicle), the ROV provides hydraulic oil for the driving oil cylinder (19), one end of the fixed cabin (8) is hermetically connected with the front cover (7), the other end of the fixed cabin is hermetically connected with one end of the storage cabin (11), the other end of the storage cabin (11) is hermetically connected with the rear cover (13), the knife rest (14) with the cutting knife (27) is installed in the fixed cabin (8), and the storage cabin (11) is provided with a putting opening (10) for putting in biological; a rigid body (24) as the output of the driving oil cylinder (19) penetrates through the rear cover (13) and then is inserted into the storage cabin (11) and is connected with one side of a piston (26), the other side of the piston (26) is connected with a cutting knife pressing plate (15) which is interlocked with the piston (26), and the piston (26) is in sealed sliding connection with the inner wall of the storage cabin (11); the fixed solution is stored in the fixed solution storage bag (1), and the fixed solution storage bag (1) is communicated with the inside of the fixed cabin (8) through a rubber tube channel (2).

2. The ROV-based large-scale organism cutting type in-situ fixing device for deep sea mussels and the like according to claim 1, wherein: one end of the tool rest (14) is of an annular structure (1401) and is mounted on the inner surface of the other end of the fixed cabin (8), two extending parts (1402) extending along the axial direction are symmetrically arranged on the end face, facing the front cover (7), of the annular structure (1401), the extending parts (1402) are of a hollow structure, the edge of one hollow side is the end face, facing the front cover (7), of the annular structure (1401), a plurality of grooves (32) are formed in the edge of the other hollow side, and a cutting knife (27) is mounted in each groove (32).

3. The ROV-based large organism cutting type in-situ fixing device for deep sea mussels and the like according to claim 2, wherein: the width of the extension parts (1402) gradually decreases towards the middle along the pushing direction of the biological sample, and an arc surface which is bent towards one side of the piston (26) is arranged between the two symmetrical extension parts (1402).

4. The ROV-based large organism cutting type in-situ fixing device for deep sea mussels and the like according to claim 2, wherein: the edge of the other side of the hollow part is in a V shape; the number of the grooves (32) on the extending parts (1402) on the two sides is equal, the grooves correspond to one another, and the two ends of each cutting knife (27) are respectively arranged in the two corresponding grooves (32).

5. The ROV-based large-scale organism cutting type in-situ fixing device for deep sea mussels and the like according to claim 1, wherein: the cutting knife pressing plate (15) is formed by stacking a plurality of pressing plates, each pressing plate is divided into a pressing plate guide plate (1501) and a pressing plate extruding part (1502), one side of the pressing plate extruding part (1502) is connected with the other side of the piston (26), a cutting knife pressing plate notch (21) with a V-shaped horizontal projection is arranged on the other side of the pressing plate extruding part (1502), the pressing plate guide plates (1501) are symmetrically arranged on two sides of the cutting knife pressing plate notch (21), and the pressing plate guide plates (1501) on each pressing plate penetrate through the space between every two adjacent cutting knives (27) in the moving process along with the piston (26).

6. The ROV-based large-scale organism cutting type in-situ fixing device for deep sea mussels and the like according to claim 1, wherein: the device is characterized in that a guide disc (25) is installed on the driving oil cylinder (19), at least one guide hole (18) is formed in the guide disc (25), a guide rod (16) penetrates through the guide hole (18), one end of the guide rod (16) is inserted into the storage cabin (11) and is connected with the piston (26) to limit the rotation of the piston (26) and the driving oil cylinder (19), and the other end of the guide rod (16) always slides in the guide hole (18).

7. The ROV-based large-scale organism cutting type in-situ fixing device for deep sea mussels and the like according to claim 1, wherein: a fixed joint (4), a pressure relief joint A (6) and a pressure relief joint B (30) are respectively arranged on the front cover (7), a fixed liquid extension pipe (31) is arranged in the fixed cabin (8), the fixed liquid extension pipe (31) is communicated with one end of the fixed joint (4), the other end of the fixed joint (4) is communicated with a fixed liquid storage bag (1) through a rubber pipe channel (2), and a fixed valve (3) is arranged on the rubber pipe channel (2); one end of the pressure relief joint A (6) is in threaded connection with the front cover (7) and communicated with the inside of the fixed cabin (8), the other end of the pressure relief joint A (6) is communicated with the outside through a pipeline, and a pressure relief valve (5) is arranged on the pipeline; one end of the pressure relief joint B (30) is in threaded connection with the front cover (7) and communicated with the inside of the fixed cabin (8), the other end of the pressure relief joint B (30) is communicated with the outside through a pipeline, and a one-way valve (29) is arranged on the pipeline.

8. The ROV-based large-scale organism cutting type in-situ fixing device for deep sea mussels and the like according to claim 1, wherein: the length of the driving oil cylinder (19) is equal to the moving distance of the rigid body (24) in the storage cabin (11) and the fixed cabin (8).

9. The ROV-based large-scale organism cutting type in-situ fixing device for deep sea mussels and the like according to claim 1, wherein: the inner surface of the tool rest (14) is provided with a piston sealing ring (12), the outer surface of the piston (26) is sleeved with a sealing O ring (28), when the driving oil cylinder (19) moves forwards to complete the process, the sealing O ring (28) on the piston (26) reaches the position of the piston sealing ring (12), and the fixed cabin (8) becomes a sealed cabin body through the sealing O ring (28) and the piston sealing ring (12).