CN112189615B - ROV-based deep sea in-situ large-scale biological stress device and use method thereof - Google Patents
ROV-based deep sea in-situ large-scale biological stress device and use method thereof Download PDFInfo
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- CN112189615B CN112189615B CN202011126985.4A CN202011126985A CN112189615B CN 112189615 B CN112189615 B CN 112189615B CN 202011126985 A CN202011126985 A CN 202011126985A CN 112189615 B CN112189615 B CN 112189615B
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- 238000011065 in-situ storage Methods 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 12
- 238000005070 sampling Methods 0.000 claims abstract description 17
- 230000035882 stress Effects 0.000 claims description 81
- 238000013016 damping Methods 0.000 claims description 20
- 238000007789 sealing Methods 0.000 claims description 18
- 238000002347 injection Methods 0.000 claims description 14
- 239000007924 injection Substances 0.000 claims description 14
- 230000004790 biotic stress Effects 0.000 claims description 8
- 238000004140 cleaning Methods 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 4
- 239000000243 solution Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000011160 research Methods 0.000 abstract description 9
- 238000002474 experimental method Methods 0.000 abstract description 4
- 230000007797 corrosion Effects 0.000 abstract description 3
- 238000005260 corrosion Methods 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 description 4
- 238000012613 in situ experiment Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007769 metal material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000237536 Mytilus edulis Species 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 229910001069 Ti alloy Inorganic materials 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000020638 mussel Nutrition 0.000 description 1
- 229910052755 nonmetal Inorganic materials 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000003938 response to stress Effects 0.000 description 1
- 239000007779 soft material Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
- A01K63/006—Accessories for aquaria or terraria
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the field of deep sea in-situ large-scale biological stress experiment research, and in particular relates to an ROV-based deep sea in-situ large-scale biological stress device and a use method thereof, wherein a stress culture barrel body and a sample bag are respectively placed on a placing disc, a fixed hoop is arranged on the stress culture barrel body, one side of an upper cover is rotationally connected with the stress culture barrel body, an upper cover switch pressing block is arranged on the other side of the upper cover, a switch lower end pressing block is arranged on the fixed hoop, and the upper cover switch pressing block is clamped with the switch lower end pressing block when the upper cover is closed with the stress culture barrel body; the syringe is arranged on the fixed hoop, the syringe is communicated with the inside of the stress culture barrel body through the syringe, and the sample bag is communicated with the syringe through the sampling tube; two ends of the tension spring are respectively connected with the upper cover and the placing disc. The invention has little influence by sampling depth, strong corrosion resistance, flexible and stable work, and can rapidly and effectively acquire, culture, stress and fix samples.
Description
Technical Field
The invention belongs to the field of deep sea in-situ large-scale biotic stress experimental research, and particularly relates to an ROV-based deep sea in-situ large-scale biotic stress device and a use method thereof.
Background
The deep sea cold spring energy ecological system has unique chemical environment and ocean dynamic characteristics, is highly similar to the environment in early earth life formation period, breeds unique ecological system and life process, and is an important target area for revealing the origin, evolution and extreme environment adaptation mechanism of life. Under the continuous support of deep sea detection platforms of units in China, the characteristics, biological compositions and potential functions of a deep sea cold spring energy ecological system are preliminarily known; however, due to the lack of a reliable large-scale biological in-situ experimental platform and the difficulty in realizing continuous dynamic observation and mechanism analysis of a deep sea key life process, the method has become a bottleneck problem which is concerned by research teams at home and abroad but restricts deep development of deep sea research for a long time.
The research on the in-situ experiment in the aspect of large-scale biotic stress culture is more in depth and the systematic research is becoming the urgent problem to be solved by combining the existing research experience at home and abroad and utilizing the accurate positioning capability of ROV (remote unmanned submersible).
Disclosure of Invention
Aiming at the technical bottleneck problem encountered in the current deep sea life science research, the invention aims to provide an ROV-based deep sea in-situ large-scale biological stress device and a use method thereof.
The aim of the invention is realized by the following technical scheme:
the stress device comprises an upper cover, a fixed hoop, a placing disc, a stress culture barrel body, a tension spring, a syringe and a sample bag, wherein the stress culture barrel body and the sample bag are respectively placed on the placing disc, the stress culture barrel body is provided with the fixed hoop, one side of the upper cover is connected with the stress culture barrel body in a relatively rotatable manner, the other side of the upper cover is provided with an upper cover switch pressing block, the fixed hoop is provided with a switch lower end pressing block, and the upper cover switch pressing block is clamped with the switch lower end pressing block when the upper cover is closed with the stress culture barrel body; the syringe is arranged on the fixed hoop, the syringe is communicated with the inside of the stress culture barrel body through a syringe tube, the sample bag is communicated with the syringe through a sampling tube, base fluid filled in the sample bag is injected into the inside of the syringe through the sampling tube, and a sample in the inside of the syringe is injected into the stress culture barrel body through the syringe tube; two ends of the tension spring are respectively connected with the upper cover and the placing disc.
Wherein: the upper cover switch pressing block is provided with pressing block upper end teeth, the switch lower end pressing block is divided into a switch lower end pressing block A and a switch lower end pressing block B, the switch lower end pressing block A is fixedly connected to the fixed hoop, the switch lower end pressing block B is rotationally connected with the switch lower end pressing block A through a rotating shaft, the rotating shaft is sleeved with a torsion spring, and two pressing edges of the torsion spring are respectively abutted to the switch lower end pressing block A and the switch lower end pressing block B; switch lower extreme teeth are arranged on the switch lower extreme briquetting B, the briquetting upper end teeth on the upper cover switch briquetting are meshed with the switch lower end teeth on the switch lower extreme briquetting B when the upper cover is closed with the stress culture barrel body.
One side of the upper cover is connected with the stress culture barrel body through a damping hinge, one end of the damping hinge is fixedly connected to the upper cover, and the other end of the damping hinge is fixedly connected to the stress culture barrel body.
And a connecting line between the projection center of the upper cover and the projection center of the upper cover switch pressing block passes through the circle center of the upper cover.
One end of the damping hinge is connected with a hinge extension plate, the lower surface of the hinge extension plate is provided with an upper end fixing ring, the placing plate is provided with a lower end fixing ring, and two ends of the tension spring are respectively hooked on the upper end fixing ring and the lower end fixing ring.
The upper cover is respectively provided with a handle A, a one-way valve and a stop ball valve.
The fixed hoops are divided into an upper fixed hoop and a lower fixed hoop, the lower end pressing block of the switch is arranged on the upper fixed hoop, and the injector is arranged on the lower fixed hoop through the injector fixing frame.
And a handle B which is obliquely arranged and is convenient for the ROV manipulator to grasp is arranged on the lower end fixing hoop.
The inner side of the upper cover is provided with a sealing gasket, and the outer edge of the sealing gasket is smaller than the outer edge of the upper cover; when the upper cover is closed and pressed, the sealing gasket is in sealing abutting connection with the stress culture barrel body.
The application method of the deep sea in-situ large-scale biological stress device based on the ROV comprises the following steps:
step one, integrally disassembling and cleaning the shore base end; the whole stress device is disassembled and then cleaned;
step two, assembling after cleaning, wherein in an initial state, the upper cover is in an open state, and the sample bag is in a base liquid loading state;
step three, carrying the stress device to a fixed point by using an ROV for operation, grabbing a deep sea large organism by using an ROV manipulator, putting the deep sea large organism into the stress culture barrel, closing the upper cover by using the ROV manipulator, clamping the upper cover switch pressing block with the switch lower end pressing block, and sealing and closing the upper cover and the stress culture barrel;
step four, pressing the injector by using an ROV manipulator, injecting a sample in the injector into the stress culture barrel body through a syringe, and recovering the injector when the ROV manipulator stops pressing the injector; injecting base solution into the syringe through the sampling tube by the sample bag, and carrying out stress culture on the large organism;
step five, after a set time, using an ROV manipulator to touch the lower end pressing block of the switch to separate the lower end pressing block of the switch from the upper cover switch pressing block, and pulling the upper cover by the tension spring to enable the damping hinge to rotate, and opening the upper cover; after opening, standing for a set time, and naturally exchanging the water body in the stress culture barrel body with the deep sea external environment;
step six, closing the upper cover by using an ROV manipulator, and repeating the step four and the step five until the experimental requirements are met;
and seventhly, opening the upper cover by using an ROV manipulator, and then finishing pouring of the sample in the stress culture barrel body by using the ROV manipulator, pouring the sample into an in-situ fixing device, fixing the sample, and then recovering the ROV carried sample.
The invention has the advantages and positive effects that:
the invention integrates the in-situ experimental device for acquiring, culturing, stressing and fixing the large-scale organisms in the deep sea, meets the requirements of symbiosis, stressing and comparison experiments of the large-scale organisms in the deep sea energy ecological system, and learns the material energy acquiring and transmitting modes of the deep sea symbiosis system through isotope addition culture; specific stress experiments are carried out in situ, stress response mechanisms are analyzed, and the stress device is slightly influenced by sampling depth, has strong corrosion resistance, is flexible and stable in work, and can rapidly and effectively acquire, culture, stress and fix samples.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a stress device according to the present invention;
FIG. 2 is a schematic perspective view of the stress incubation tub of FIG. 1 with components mounted thereon;
FIG. 3 is a bottom view of the structure of the upper cover of the stress device of the present invention;
FIG. 4 is a schematic view of the torsion spring of the stress device of the present invention;
wherein: 1 is a handle A,2 is a check valve, 3 is a stop ball valve, 4 is a damping hinge, 5 is an upper end fixed ring, 6 is a hinge extension plate, 7 is an upper end fixed hoop, 8 is a lower end fixed hoop, 9 is a handle B,10 is a placing disc, 11 is a tension spring, 12 is a syringe fixing frame, 13 is a lower end fixed ring, 14 is a syringe, 15 is a syringe, 16 is an injection port, 17 is a sampling tube, 18 is a sample bag, 19 is an upper cover, 20 is a check valve upper cover port, 21 is a stop ball valve upper cover port, 22 is a sealing gasket inner edge, 23 is a sealing gasket, 24 is a sealing gasket outer edge, 25 is an upper cover switch pressing block, 26 is a pressing block upper end tooth, 27 is a switch lower end tooth, 28 is a rotating shaft hole, 29 is a switch lower end pressing block A,30 is a torsion spring pressing edge A,31 is a torsion spring pressing edge B,32 is a switch lower end pressing block B,33 is a torsion spring, 34 is a rotating shaft, and 35 is a culture barrel body.
Detailed Description
The invention is described in further detail below with reference to the accompanying drawings.
As shown in fig. 1 to 4, the stress device comprises an upper cover 19, a fixed hoop, a placing disc 10, a stress culture barrel 35, a tension spring 11, a syringe 14 and a sample bag 18, wherein the stress culture barrel 35 and the sample bag 18 are respectively placed on the placing disc 10, the stress culture barrel 35 is provided with the fixed hoop, one side of the upper cover 19 is rotatably connected with the stress culture barrel 35, the other side of the upper cover 19 is provided with an upper cover switch pressing block 25, the fixed hoop is provided with a switch lower end pressing block, and the upper cover switch pressing block 25 is clamped with the switch lower end pressing block when the upper cover 19 is closed with the stress culture barrel 35; the injector 14 is arranged on the fixed hoop, the injector 14 is communicated with the inside of the stress culture barrel 35 through the injection tube 15, the sample bag 18 is communicated with the injector 14 through the sampling tube 17, the base solution filled in the sample bag 18 is injected into the inside of the injector 14 through the sampling tube 17, and the sample in the injector 14 is injected into the stress culture barrel 35 through the injection tube 15; both ends of the tension spring 11 are respectively connected with the upper cover 19 and the placing tray 10.
The upper cover 19 of this embodiment is circular, and one side of the upper cover 19 is connected with the stress culture barrel 35 through the damping hinge 4, and one end of the damping hinge 4 is welded on the upper cover 19, and the other end is fixedly connected on the stress culture barrel 35 by using a bolt. One end of the damping hinge 4 is welded with a hinge extension plate 6, the lower surface of the hinge extension plate 6 is provided with an upper end fixing ring 5, the placing disc 10 is provided with a lower end fixing ring 13, and two ends of the tension spring 11 are respectively hooked on the upper end fixing ring 5 and the lower end fixing ring 13.
The fixing hoop of this embodiment is divided into an upper fixing hoop 7 and a lower fixing hoop 8, the upper fixing hoop 7 is welded on the stress culture barrel 35 at the lower part of the tail end of the hinge extension plate 6, one side of the lower fixing hoop 8 is welded with a handle B9, the handle B9 is obliquely arranged, the ROV manipulator is convenient to grasp, the other side of the lower fixing hoop 8 is welded with the injector fixing frame 12, and the injector 14 is installed on the injector fixing frame 12. The outlet of the lower end of the injector 14 of the embodiment is connected with one end of an injection tube 15, the other end of the injection tube 15 is connected with an injection port 16, and the injection port 16 is in threaded connection with the middle lower part of a stress culture barrel 35; the upper end of the syringe 14 is connected to one end of the sampling tube 17, the other end of the sampling tube 17 is connected to the sample bag 18, and the sample bag 18 is fixed to the placing tray 10. The syringe 15 and the sampling tube 17 of this embodiment are manufactured by saint-gobain (san gobain) corporation, france, the stress culture barrel 35 is entirely made of a non-metal material (such as teflon), the sample bag 18 is made of a soft material (such as plastic), the syringe 14 is made of the existing product of "pastoral adjustable continuous syringe", and the injection value of each injection is adjustable.
The upper cover switch pressing block 25 of the embodiment is provided with a pressing block upper end tooth 26. The lower end pressing block of the switch of the embodiment is divided into a lower end pressing block A29 of the switch and a lower end pressing block B32 of the switch, the lower end pressing block A29 of the switch is welded on the upper end fixing hoop 7, the lower end pressing block B32 of the switch and the lower end pressing block A29 of the switch are fixedly connected with a rotating shaft 33 through a rotating shaft hole 28, the lower end pressing block B32 of the switch is rotatably connected with the lower end pressing block A29 of the switch through the rotating shaft 33, a torsion spring 34 is sleeved on the rotating shaft 33, two pressing edges (namely a torsion spring pressing edge A30 and a torsion spring pressing edge B31) of the torsion spring 34 are respectively abutted against the lower end pressing block A29 of the switch and the lower end pressing block B32 of the switch, the torsion spring 34 keeps tension, and the lower end pressing block A29 of the switch and the lower end pressing block B32 of the switch are separated; the switch lower end press block B32 is provided with switch lower end teeth 27, and when the upper cover 19 is closed with the stress culture barrel 35, the press block upper end teeth 26 on the upper cover switch press block 25 are meshed with the switch lower end teeth 27 on the switch lower end press block B32. The connecting line between the projection center of the upper cover 19 and the projection center of the upper cover switch pressing block 25 of one end of the damping hinge 4 in this embodiment passes through the center of the upper cover 19, so that the upper end tooth 26 of the pressing block above the upper end switch pressing block 25 is conveniently meshed with the lower end tooth 27 of the switch on the lower end switch pressing block B32 in the rotating process of the damping hinge 4.
The inner side of the upper cover 19 of the embodiment is provided with a sealing gasket 23, and the sealing gasket outer edge 24 of the sealing gasket 23 is smaller than the outer edge of the upper cover 19; when the upper cover 19 is closed and pressed, the sealing gasket 23 contacts with the stress culture barrel 35 to complete the sealing function. The upper cover 19 is respectively provided with a check valve upper cover port 20 and a stop ball valve upper cover port 21, the check valve 2 and the stop ball valve 3 are respectively connected by threads, liquid in the stress culture barrel 35 can be discharged through the check valve 2, and oxygen can be supplied into the rib forced culture barrel 35 through the stop ball valve 3. The check valve 2 and the stop ball valve 3 of the embodiment are processed by adopting metal materials (such as Ti alloy) so as to avoid sample pollution. A handle A1 is welded in the middle of the upper cover 1, so that the ROV manipulator can conveniently grasp the handle.
The application method of the deep sea in-situ large-scale biological stress device based on the ROV comprises the following steps:
step one, integrally disassembling and cleaning the shore base end; the whole stress device is disassembled and then cleaned;
step two, assembling after cleaning, wherein in an initial state, the upper cover 19 is in an open state, and the sample bag 18 is in a base fluid loading state;
step three, using an ROV to carry a stress device to reach a fixed point for operation, using an ROV manipulator to grasp a deep sea large organism, putting the deep sea large organism into a stress culture barrel body 35, using the ROV manipulator to grasp a handle A1 so as to close an upper cover 19, wherein a press block upper end tooth 26 on an upper cover switch press block 25 is meshed with a switch lower end tooth 27 on a switch lower end press block B32, and when the upper cover 19 is closed, the upper cover 19 and the stress culture barrel body 35 are sealed and closed;
step four, pressing the injector 14 by using an ROV manipulator, wherein a sample in the injector 14 reaches the injection port 16 through the injection tube 15, and is injected into the stress culture barrel 35, the liquid in the stress culture barrel 35 can be discharged through the one-way valve 2, and when the ROV manipulator stops pressing the injector 14, the injector 14 can be restored; the sample bag 18 is filled with the base liquid into the syringe 14 through the sampling tube 17, and can be sequentially and reciprocally circulated, and injection is stopped after the designed quantity is reached, so that stress culture of the large organism is performed;
step five, after a set time, an ROV manipulator is used for touching a switch lower end pressing block B32, so that a switch lower end tooth 27 on the switch lower end pressing block B32 is separated from a pressing block upper end tooth 26 on an upper cover switch pressing block 25 against the elastic action of a torsion spring 34, and the tension spring 11 pulls the upper cover 19, so that the damping hinge 4 rotates and the upper cover 19 is opened; the opening speed of the upper cover 19 is greatly slowed down due to the damping action of the damping hinge 4; after opening, standing for a set time, and naturally exchanging the water body inside the stress culture barrel body 35 with the deep sea external environment, wherein the exchange time is related to the volume of the stress culture barrel body 35 and the nearby flow rate;
step six, grabbing the handle A1 by using an ROV manipulator to further close the upper cover 19, and repeating the step four and the step five until the experimental requirements are met;
and step seven, opening the upper cover 19 by using an ROV manipulator, grabbing the handle B9 by using the ROV manipulator, pouring the sample in the stress culture barrel 35, pouring the sample into an in-situ fixing device, fixing the sample, and then recovering the ROV carried sample.
The in-situ fixing device can be a large-scale biological extrusion-type in-situ fixing device based on ROV, such as deep sea mussels, and the like, with the authorized bulletin number of CN211042749U and 7/17/2020.
Aiming at the technical bottleneck of the current deep sea life science research, the invention breaks through the in-situ accurate base solution injection technology, breaks through the key technologies of quick uncovering, underwater in-situ fixation and the like under an in-situ closed system, finally completes in-situ experiments, and solves the problems that the in-situ experiments of large-scale organisms in China are insufficient, and the in-situ forced culture and the fixed transfer of the large-scale organisms are difficult. The stress device disclosed by the invention is combined with an underwater ROV to operate, runs stably, is convenient to carry by using the ROV, can be suitable for complex submarine environments in 6000m depth, temperature and ocean current environments, integrates the functions of acquisition, culture and stress, and finally cooperates with the fixing device to meet the requirements of symbiosis, stress and comparison experiments of large organisms in a deep sea chemical energy ecological system, and has the advantages that the stress device is less influenced by the sampling depth, strong in corrosion resistance, flexible and stable in work, and can be used for rapidly and effectively acquiring, culturing, stressing and fixing samples.
Claims (6)
1. An ROV-based deep sea in-situ large-scale biological stress device is characterized in that: the device comprises an upper cover (19), a fixed hoop, a placing disc (10), a stress culture barrel body (35), a tension spring (11), a syringe (14) and a sample bag (18), wherein the stress culture barrel body (35) and the sample bag (18) are respectively placed on the placing disc (10), the stress culture barrel body (35) is provided with the fixed hoop, one side of the upper cover (19) is connected with the stress culture barrel body (35) in a relatively rotatable manner, the other side of the upper cover is provided with an upper cover switch pressing block (25), a switch lower end pressing block is arranged on the fixed hoop, and the upper cover switch pressing block (25) is clamped with the switch lower end pressing block when the upper cover (19) and the stress culture barrel body (35) are closed; the injector (14) is arranged on the fixed hoop, the injector (14) is communicated with the inside of the stress culture barrel body (35) through a syringe tube (15), the sample bag (18) is communicated with the injector (14) through a sampling tube (17), the base fluid filled in the sample bag (18) is injected into the inside of the injector (14) through the sampling tube (17), and the sample in the injector (14) is injected into the stress culture barrel body (35) through the syringe tube (15); two ends of the tension spring (11) are respectively connected with the upper cover (19) and the placing disc (10);
the upper cover switch pressing block (25) is provided with a pressing block upper end tooth (26), the switch lower end pressing block is divided into a switch lower end pressing block A (29) and a switch lower end pressing block B (32), the switch lower end pressing block A (29) is fixedly connected to the fixed hoop, the switch lower end pressing block B (32) is rotationally connected with the switch lower end pressing block A (29) through a rotating shaft (33), the rotating shaft (33) is sleeved with a torsion spring (34), and two pressing edges of the torsion spring (34) are respectively abutted against the switch lower end pressing block A (29) and the switch lower end pressing block B (32); the switch lower end pressing block B (32) is provided with switch lower end teeth (27), and the pressing block upper end teeth (26) on the upper cover switch pressing block (25) are meshed with the switch lower end teeth (27) on the switch lower end pressing block B (32) when the upper cover (19) is closed with the stress culture barrel body (35);
one side of the upper cover (19) is connected with the stress culture barrel body (35) through a damping hinge (4), one end of the damping hinge (4) is fixedly connected to the upper cover (19), and the other end of the damping hinge is fixedly connected to the stress culture barrel body (35);
a connecting line between the projection center of one end of the damping hinge (4) on the upper cover (19) and the projection center of the upper cover switch pressing block (25) on the upper cover (19) passes through the circle center of the upper cover (19);
one end of the damping hinge (4) is connected with a hinge extension plate (6), the lower surface of the hinge extension plate (6) is provided with an upper end fixing ring (5), the placing disc (10) is provided with a lower end fixing ring (13), and two ends of the tension spring (11) are respectively hooked on the upper end fixing ring (5) and the lower end fixing ring (13).
2. The ROV-based deep sea in-situ large biotic stress device according to claim 1, characterized in that: the upper cover (19) is respectively provided with a handle A (1), a one-way valve (2) and a stop ball valve (3).
3. The ROV-based deep sea in-situ large biotic stress device according to claim 1, characterized in that: the fixed hoops are divided into an upper end fixed hoop (7) and a lower end fixed hoop (8), the switch lower end pressing block is arranged on the upper end fixed hoop (7), and the injector (14) is arranged on the lower end fixed hoop (8) through the injector fixing frame (12).
4. The ROV-based deep sea in-situ large biotic stress device as claimed in claim 3 wherein: the lower end fixed hoop (8) is provided with a handle B (9) which is obliquely arranged and is convenient for the ROV manipulator to grasp.
5. The ROV-based deep sea in-situ large biotic stress device according to claim 1, characterized in that: a sealing gasket (23) is arranged on the inner side of the upper cover (19), and the outer edge (24) of the sealing gasket (23) is smaller than the outer edge of the upper cover (19); when the upper cover (19) is closed and pressed, the sealing gasket (23) is in sealing abutting connection with the stress culture barrel body (35).
6. A method of using an ROV-based deep sea in situ macro biotic stress device as claimed in any of claims 1 to 5 wherein:
step one, integrally disassembling and cleaning the shore base end; the whole stress device is disassembled and then cleaned;
step two, assembling after cleaning, wherein in an initial state, the upper cover (19) is in an open state, and the sample bag (18) is in a base fluid loading state;
step three, carrying the stress device to a fixed point by using an ROV for operation, grabbing a deep sea large organism by using an ROV manipulator, putting the deep sea large organism into the stress culture barrel body (35), closing the upper cover (19) by using the ROV manipulator, clamping an upper cover switch pressing block (25) with a switch lower end pressing block, and sealing and closing the upper cover (19) with the stress culture barrel body (35);
pressing the injector (14) by using an ROV manipulator, injecting a sample inside the injector (14) into the stress culture barrel body (35) through the injection tube (15), and recovering the injector (14) when the ROV manipulator stops pressing the injector (14); the sample bag (18) is used for injecting base solution into the syringe (14) through the sampling tube (17) to perform stress culture of the large organism;
step five, after a set time, using an ROV mechanical arm to touch the lower end pressing block of the switch to separate the lower end pressing block of the switch from the upper cover switch pressing block (25), and pulling the upper cover (19) by the tension spring (11) to rotate the damping hinge (4), and opening the upper cover (19); after opening, standing for a set time, and naturally exchanging the water body in the stress culture barrel body (35) with the deep sea external environment;
step six, closing the upper cover (19) by using an ROV manipulator, and repeating the step four and the step five until the experimental requirements are met;
and seventhly, opening the upper cover (19) by using an ROV manipulator, pouring the sample in the stress culture barrel body (35) by using the ROV manipulator, pouring the sample into an in-situ fixing device, fixing the sample, and then recovering the ROV carried sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011126985.4A CN112189615B (en) | 2020-10-20 | 2020-10-20 | ROV-based deep sea in-situ large-scale biological stress device and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011126985.4A CN112189615B (en) | 2020-10-20 | 2020-10-20 | ROV-based deep sea in-situ large-scale biological stress device and use method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112189615A CN112189615A (en) | 2021-01-08 |
| CN112189615B true CN112189615B (en) | 2023-09-19 |
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