CN207502550U - A kind of Biogeochemistry original position experimental provision for deep-sea - Google Patents

A kind of Biogeochemistry original position experimental provision for deep-sea Download PDF

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Publication number
CN207502550U
CN207502550U CN201721742519.2U CN201721742519U CN207502550U CN 207502550 U CN207502550 U CN 207502550U CN 201721742519 U CN201721742519 U CN 201721742519U CN 207502550 U CN207502550 U CN 207502550U
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China
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reagent
tracer
bag
seawater
terminator
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CN201721742519.2U
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杜梦然
薛帅
彭晓彤
张健
吴邦春
王杰
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Institute of Deep Sea Science and Engineering of CAS
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Institute of Deep Sea Science and Engineering of CAS
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Abstract

The utility model discloses a kind of Biogeochemistry original position experimental provisions for deep-sea, including seawater sampling device, reagent and culture bag system, reagent selection proportioning device and control device, following 5 kinds of functions can be realized by the effect of peristaltic pump, solenoid valve and rotary valve:Seawater is into culture bag, reagent quantitative, reagent is terminated into culture bag, the cleaning of reagent flow path, experiment and terminator cleaning.The existing indoor experimentation of experiment is placed seabed with method and implemented in situ by this programme, achievees the purpose that abyssopelagic organism geochemistry experiment device in deep sea in-situ timing, quantitative automatic progress sample culturing and labelling experiment.Biogeochemistry original position to be recycled experimental provision, take out culture bag therein, and in the changes of contents of experiment lab analysis reactant, reaction product and isotopic tracer, the experimental datas such as the Biogeochemistry dynamics of deep sea in-situ environment can be obtained, data supporting and foundation are provided to disclose marine biogeochemistry recycle recovery.

Description

A kind of Biogeochemistry original position experimental provision for deep-sea
Technical field
The utility model is related to Biogeochemistry culture experiment technical fields, more particularly to a kind of biology for deep-sea Geochemistry original position experimental provision.
Background technology
With the reach of science, more and more problem in science focus on marine field, including Biogeochemistry Etc. relevant field.
Current most experiments sample is to be carried the samples such as seawater from seabed by sampling modes such as sampler, device for fetching water To sea, biochemistry culture and analysis are finally carried out in laboratory.
But in this process, sample has disengaged from original low temperature, high pressure living environment, the discussion that the validity of sample has.
By the investigation of early period, rarely has the relevant report of the geochemical experimental provision in situ of similar abyssopelagic organism at present. Therefore, with reference to the engineering demand of early period and principle designing scheme, a kind of Biogeochemistry for deep-sea of research and development is tested in situ Device provides experiment porch to explore the interaction mechanism between marine biogeochemistry.
Utility model content
According to Biogeochemistry in-situ observation scientific research demand, the utility model proposes a kind of biological earth for deep-sea The existing indoor experimentation of experiment can be placed seabed with method and implemented in situ, reached by chemical in situ experimental provision Abyssopelagic organism geochemistry experiment device is in deep sea in-situ timing, the quantitative automatic purpose for carrying out sample culturing and labelling experiment.
To achieve the above object, the utility model provides following technical solution:
A kind of Biogeochemistry original position experimental provision for deep-sea, including:Seawater sampling device, reagent and culture bag System, reagent selection-proportioning device and control device;
The reagent includes cultivate reagent bag, tracer reagent bag and terminator reagent bag with culture bag system;
The control device connects respectively with the seawater sampling device and reagent selection-proportioning device communication;Institute Electric signal can be sent to the seawater sampling device and reagent selection-proportioning device by stating control device, make seawater, described Terminator in tracer and the terminator reagent bag in tracer reagent bag successively enters the cultivate reagent bag.
Preferably, the seawater sampling device includes seawater housing and the seawater peristaltic pump and electromagnetism that set within it Valve;The entrance of the seawater peristaltic pump can be contacted by the seawater housing with the external world, and the solenoid valve is arranged on the sea Channel between water wriggling pump discharge and the cultivate reagent bag.
Preferably, the quantity of the cultivate reagent bag is multiple, each cultivate reagent bag and the seawater peristaltic pump Channel is both provided with the solenoid valve between outlet.
Preferably, the seawater housing includes cavity and the trepanning end cap and end cover that are separately mounted to its both ends; The entrance of the seawater peristaltic pump can be contacted by the trepanning end cap with the external world.
Preferably, the tracer that the reagent selection-proportioning device includes reagent housing and sets within it quantitatively revolves Rotary valve;The tracer quantifies in rotary valve and is provided with quantitative loop;
The seawater sampling device further includes the tracer solenoid valve being arranged in the seawater housing and tracer is wriggled Pump;The entrance of the tracer peristaltic pump is communicated in the tracer reagent bag;
The tracer quantifies rotary valve and includes multiple channels:The tracer peristaltic pump is communicated in into tracer channel Outlet, waste discharge channel are communicated in waste fluid bag, and sea water passage is communicated on the outlet of the seawater peristaltic pump and channel and sets Tracer solenoid valve is stated, culture bag channel is communicated in the cultivate reagent bag.
Preferably, the quantity of the tracer reagent bag is multiple;
Reagent selection-the proportioning device further includes the tracer selection rotary valve being arranged in the reagent housing;
The tracer selection rotary valve includes multiple channels:Multiple tracer selector channels one-to-one correspondence are communicated in multiple The tracer reagent bag, tracer channel are communicated in the entrance of the tracer peristaltic pump, can pass through institute into sea water passage It states reagent housing and is communicated in the external world.
Preferably, the quantity of the cultivate reagent bag is multiple;
Reagent selection-the proportioning device further includes the tracer selection culture rotary valve being arranged in the reagent housing;
The tracer selection culture rotary valve includes multiple channels:Multiple culture bag selector channels one-to-one correspondence are communicated in more A cultivate reagent bag, tracer channel are communicated in the culture bag channel that the tracer quantifies rotary valve, and waste discharge channel connects Pass through waste fluid bag.
Preferably, the terminator that the reagent selection-proportioning device includes reagent housing and sets within it quantitatively revolves Rotary valve;The terminator quantifies in rotary valve and is provided with quantitative loop;
The seawater sampling device further includes the terminator solenoid valve being arranged in the seawater housing and terminator is wriggled Pump;The outlet of the terminator peristaltic pump is in the terminator reagent bag;
The terminator quantifies rotary valve and includes multiple channels:Terminator channel is communicated in the terminator reagent bag, compacted Dynamic pump channel is communicated in the entrance of the terminator peristaltic pump, and sea water passage is communicated in the outlet of the seawater peristaltic pump and channel On be provided with the terminator solenoid valve, culture bag channel is communicated in the cultivate reagent bag.
Preferably, the quantity of the cultivate reagent bag is multiple;
Reagent selection-the proportioning device further includes the termination selection culture rotary valve being arranged in the reagent housing;
The selection culture rotary valve that terminates includes multiple channels:Multiple culture bag selector channels one-to-one correspondence are communicated in more A cultivate reagent bag, terminator channel are communicated in the culture bag channel that the terminator quantifies rotary valve, and waste discharge channel connects Pass through waste fluid bag.
Preferably, the reagent housing includes cavity and the trepanning end cap and end cover that are separately mounted to its both ends.
It can be seen from the above technical scheme that the Biogeochemistry provided by the utility model for deep-sea is in situ real Experiment device mainly utilizes the process experiments data such as tracer method, the Biogeochemistry dynamics to obtain abyssal environment. In practice, Biogeochemistry original position experimental provision can, timing automatic in deep sea in-situ, quantitatively by reactant and Isotopic tracer is separately added into the seawater sample in target culture bag, is occurred automatically in tracer element label in situ environment Biogeochemical reaction process (such as nitration reaction and anti-nitration reaction).Treat that biogeochemical reaction proceeds to centainly mistake Cheng Hou, Biogeochemistry original position experimental provision add in quantitative biogeochemical reaction according to scheduled time course and terminate Agent (biostatic agent), to inhibit the biogeochemical reaction in culture bag, reactant, reaction product and isotopic tracer It is stored in culture bag.Biogeochemistry original position to be recycled experimental provision takes out culture bag therein, and divides in laboratory The changes of contents of reactant, reaction product and isotopic tracer is analysed, the Biogeochemistry that can obtain deep sea in-situ environment is moved The experimental datas such as mechanics provide data supporting and foundation to disclose marine biogeochemistry recycle recovery.
Description of the drawings
It in order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor Under, it can also be obtained according to these attached drawings other attached drawings.
Fig. 1 is the structure diagram of experimental provision totality that the utility model embodiment provides;
Fig. 2 is the structure diagram of seawater sampling device that the utility model embodiment provides;
Fig. 3 is the structure diagram of reagent selection-proportioning device that the utility model embodiment provides;
Fig. 4 is the work flow path principle sketch of experimental provision that the utility model embodiment provides.
Wherein, 1 is seawater sampling device, and 5 be trepanning end cap, and 6 be pull rod, and 7 be mounting disc, and 8 be tracer solenoid valve, 9 For terminator solenoid valve, 10,11,17,18,19,20,22,23,24 and 25 between culture bag and seawater peristaltic pump channel electricity Magnet valve, 12 be pull rod, and 13 be mounting disc, and 14 be pull rod, and 15 be cavity, and 16 be end cover, and 21 be terminator peristaltic pump, and 26 are Seawater peristaltic pump, 27 be tracer peristaltic pump;
2 be reagent and culture bag system;
3 be reagent selection-proportioning device, and 28 be trepanning end cap, and 29 be pull rod, and 30 be pull rod, and 31 be mounting disc, and 32 be chamber Body, 33 be end cover, and 34 select rotary valve for tracer, and 35 quantify rotary valve for tracer, and 36 be tracer selection culture rotation Rotary valve, 37 quantify rotary valve for terminator, and 38 is terminate selection culture rotary valve;
4 devices in order to control.
Specific embodiment
The following is a combination of the drawings in the embodiments of the present utility model, and the technical scheme in the embodiment of the utility model is carried out It clearly and completely describes, it is clear that the described embodiments are only a part of the embodiments of the utility model rather than whole Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are without making creative work All other embodiments obtained shall fall within the protection scope of the present invention.
The Biogeochemistry original position experimental provision for deep-sea that the utility model embodiment provides, core improvement It is, including:Seawater sampling device 1, reagent and culture bag system 2, reagent selection-proportioning device 3 and control device 4, knot Structure is referred to shown in Fig. 1;
Reagent includes cultivate reagent bag, tracer reagent bag and terminator reagent bag with culture bag system 2;
Control device 4 connects respectively with seawater sampling device 1 and reagent selection-communication of proportioning device 3;4 energy of control device Electric signal is enough sent to seawater sampling device 1 and reagent selection-proportioning device 3, makes seawater, the tracer in tracer reagent bag Successively enter cultivate reagent bag with the terminator in terminator reagent bag.
It can be seen from the above technical scheme that the Biogeochemistry for deep-sea that the utility model embodiment provides Experimental provision in situ can carry out the work(such as quantitative sample injection, timing-quantitative sample injection of reagent and termination experiment automatically in deep sea in-situ Can, realize full-automatic Biochemistry Experiment in deep sea in-situ.
Preferably, seawater sampling device 1 includes seawater housing and the seawater peristaltic pump 26 and electromagnetism that set within it Valve;There is seawater housing pressure-resistance structure can provide protection for wherein component;The entrance of seawater peristaltic pump 26 can pass through seawater Housing is contacted with the external world, and solenoid valve is arranged on seawater peristaltic pump 26 and exports channel between cultivate reagent bag.Using peristaltic pump Compared with other sample introduction mechanisms, has the following advantages:It is not contacted with fluid media (medium), so as to avoid influencing testing, while flow velocity is steady It is fixed, adapt to hyperbaric environment work.
Further, the quantity of cultivate reagent bag is multiple, between each cultivate reagent bag and the outlet of seawater peristaltic pump 26 Channel is both provided with solenoid valve, and structure is referred to shown in Fig. 2 and Fig. 4.So set, multiple comparison trainings can be realized simultaneously Support experiment.When needing seawater into culture bag, control device 4 sends electric signal to seawater sampling device 1, seawater sampling device 1 Interior seawater peristaltic pump 26 and target culture bag corresponding channel solenoid valve are opened, and external seawater is under 26 swabbing action of seawater peristaltic pump Into target cultivate reagent bag.
Specifically, seawater housing includes cavity 15 and is separately mounted to the trepanning end cap 5 at its both ends and end cover 16; The entrance of seawater peristaltic pump 26 can be contacted by trepanning end cap 5 with the external world.Its structure is referred to shown in Fig. 2, seawater sample introduction Device 1 mainly includes being mounted on the trepanning end cap 5 at the top of device, and trepanning end cap 5 is mounted on cavity 15, the bottom peace of cavity 15 Equipped with end cover 16, trepanning end cap 5, cavity 15 and end cover 16 are fixed by pull rod 14;It is installed on trepanning end cap 5 Pull rod 6,6 lower end of pull rod connect mounting disc 7, peristaltic pump 22 and peristaltic pump 23 are mounted in mounting disc 7;It is mounted under mounting disc 7 Solenoid valve 8,9,10,11,22,23,24 and 25;Pull rod 12 is installed in 7 lower part of mounting disc, and mounting disc 13 passes through pull rod 12 and mounting disc 7 connections are fixed;Peristaltic pump 21 and solenoid valve 17,18,19,20 are installed, above-mentioned arrangement is compact-sized in mounting disc 13.
It can be by the way that the amount of tracer in tracer reagent bag be controlled to be quantitatively adding to realize.Preferably implement in this programme In example, the tracer that reagent selection-proportioning device 3 includes reagent housing and sets within it quantifies rotary valve 35;The tracer Agent quantifies in rotary valve 35 and is provided with quantitative loop between entrance and exit, and tracer is more precisely quantitatively adding to realize;
Seawater sampling device 1 further includes the tracer solenoid valve 8 being arranged in seawater housing and tracer peristaltic pump 27;Show The entrance of track agent peristaltic pump 27 is communicated in tracer reagent bag;
Tracer quantifies rotary valve 35 and includes multiple channels:Going out for tracer peristaltic pump 27 is communicated in into tracer channel Mouthful, waste discharge channel is communicated in waste fluid bag (avoiding influencing environment with recycling), and sea water passage is communicated in the outlet of seawater peristaltic pump 26 And tracer solenoid valve 8 is provided on channel, culture bag channel is communicated in cultivate reagent bag.It is cut using rotary valve and other pipelines It changes structure to compare, has the advantages that small, integrated level is high and low in energy consumption;
By above structure, when needing reagent quantitative function, control device 4 sends electric signal to seawater sample introduction and fills respectively Put 1 and reagent selection-proportioning device 3;Tracer in reagent selection-proportioning device 3 quantifies rotary valve 35 and selects exit passageway Pass to waste discharge channel, access road is gated into tracer channel, and tracer peristaltic pump 27 is opened in seawater sampling device 1, will Tracer is drawn into pipeline in tracer reagent bag, and the quantitative loop of rotary valve in pipeline 35 is full of;
When needing reagent into culture bag function, control device 4 sends electric signal to seawater sampling device 1 and reagent and selects Select-proportioning device 3;Tracer in reagent selection-proportioning device 3 quantifies 35 exit passageway of rotary valve and switches to cultivate reagent Bag, access road switch to sea water passage, and the seawater peristaltic pump 26 and tracer solenoid valve 8 in seawater sample introduction control device 1 are opened It opens, under the swabbing action of seawater peristaltic pump 26, tracer is quantified the quantitative tracer in 35 quantitative loop of rotary valve and taken by seawater Band enters cultivate reagent bag;
When needing reagent flow path cleaning function, control device 4 sends electric signal to seawater sampling device 1 and reagent and selects Select-proportioning device 3;Tracer in reagent selection-proportioning device 3, which quantifies rotary valve 35 and gates exit passageway to waste discharge, to be led to Road, access road are gated into tracer channel, and tracer peristaltic pump 27 is opened in seawater sampling device 1, is wriggled in tracer Seawater replaces the tracer filled in former pipeline, so as to carry out other experiments again full of entire flow path under the suction of pump 27.
Further, to be multiple, structure is referred to shown in Fig. 4 the quantity of tracer reagent bag, from meeting different realities The needs tested;
Reagent selection-proportioning device 3 further includes the tracer selection rotary valve 34 being arranged in reagent housing;
Tracer selection rotary valve 34 includes multiple channels:Multiple tracer selector channels one-to-one correspondence are communicated in multiple Tracer reagent bag, tracer channel are communicated in the entrance of tracer peristaltic pump 27, can pass through reagent housing into sea water passage It is communicated in the external world.I.e. the entrance of tracer peristaltic pump 27 selects rotary valve 34 to be communicated in each tracer reagent bag by tracer. When needing reagent quantitative function, reagent gates target tracer reagent bag channel, outlet gating tracer into 34 entrance of rotary valve Agent channel, so as to which tracer in target tracer reagent bag is drawn into pipeline;When needing reagent flow path cleaning function, examination Agent gates access road into sea water passage into rotary valve 34, and outlet is gated to tracer channel.
Specifically, the quantity of cultivate reagent bag is multiple, structure is referred to shown in Fig. 4, can be realized simultaneously multiple Compare culture experiment;
Reagent selection-proportioning device 3 further includes the tracer selection culture rotary valve 36 being arranged in reagent housing;Tracer is selected It selects culture rotary valve 36 and includes multiple channels:Multiple culture bag selector channels correspond and are communicated in multiple cultivate reagent bags, show Track agent channel is communicated in tracer and quantifies rotary valve 35, and waste discharge channel is communicated in waste fluid bag (avoiding influencing environment with recycling).I.e. Tracer quantifies rotary valve 35 and culture rotary valve 36 is selected to be communicated in each cultivate reagent bag by tracer.Reagent is being needed into culture During bag function, exit passageway is adjusted to target culture bag selector channel by tracer selection culture rotary valve 36, access road select to Tracer channel, so as to which quantitative tracer is carried along into target cultivate reagent bag by seawater.
It can be by the way that the amount of terminator in terminator reagent bag be controlled to be quantitatively adding to realize.Preferably implement in this programme In example, the terminator that reagent selection-proportioning device 3 includes reagent housing and sets within it quantifies rotary valve 37;The termination Agent quantifies in rotary valve 37 and is provided with quantitative loop between entrance and exit, and terminator is more precisely quantitatively adding to realize;
Seawater sampling device 1 further includes the terminator solenoid valve 9 being arranged in seawater housing and terminator peristaltic pump 21;Eventually Only the outlet of agent peristaltic pump 21 is in terminator reagent bag;
Terminator quantifies rotary valve 37 and includes multiple channels:Terminator channel is communicated in terminator reagent bag, and peristaltic pump leads to Road is communicated in the entrance of terminator peristaltic pump 21, and sea water passage is communicated on the outlet of seawater peristaltic pump 26 and channel and is provided with end Only agent solenoid valve 9, culture bag channel are communicated in cultivate reagent bag.
By above structure, when needing to test expiry feature, control device 4 sends an electrical signal to seawater sampling device 1 With reagent selection-proportioning device 3;Terminator quantifies rotary valve 37 and gates access road to end in reagent selection-proportioning device 3 Only agent channel gates exit passageway to wriggling pump channel, and the terminator peristaltic pump 21 in seawater sampling device 1 is opened, will be eventually Only agent quantifies the quantitative loop of rotary valve 37 full of terminator;Then, terminator quantifies rotary valve 37 and gates exit passageway to training Bag channel is supported, access road is gated to sea water passage, seawater peristaltic pump 26 and terminator solenoid valve 9 are opened, and are wriggled in seawater Seawater enters flow path under the suction of pump 26, and terminator is quantified terminator in 37 quantitative loop of rotary valve brings culture bag into.
Further, the quantity of cultivate reagent bag is multiple, and structure is referred to shown in Fig. 4, can be realized simultaneously more A comparison culture experiment;
Reagent selection-proportioning device 3 further includes the termination selection culture rotary valve 38 being arranged in reagent housing;Terminate choosing It selects culture rotary valve 38 and includes multiple channels:Multiple culture bag selector channels correspond and are communicated in multiple cultivate reagent bags, eventually Only agent channel is communicated in the culture bag channel that terminator quantifies rotary valve 37, and waste discharge channel is communicated in waste fluid bag and (is avoided with recycling Influence environment).I.e. terminator quantifies rotary valve 37 and is communicated in each cultivate reagent bag by terminating selection culture rotary valve 38.
So set, when needing terminator cleaning function, control device 4 sends an electrical signal to 1 He of seawater sampling device Reagent selection-proportioning device 3;Terminator quantifies rotary valve 37 and gates exit passageway to culture in reagent selection-proportioning device 3 Bag channel gates access road to sea water passage, terminates selection culture rotary valve 38 and gates exit passageway to waste discharge channel, Access road is gated to terminator channel, seawater peristaltic pump 26 and terminator solenoid valve 9 are opened in seawater sampling device 1, Seawater enters flow path under the suction of seawater peristaltic pump 26, and the terminator in pipeline is discharged by waste discharge mouth
Specifically, reagent housing includes cavity 32 and the trepanning end cap 28 and end cover that are separately mounted to its both ends 33, structure is referred to shown in Fig. 3.
This programme is described further with reference to specific embodiment:
As shown in Figure 1, a kind of Biogeochemistry original position experimental provision for deep-sea, including four parts, respectively Seawater sampling device 1, reagent and culture bag system 2, reagent selection-proportioning device 3 and control device 4.Control device 4 and seawater It is powered between sampling device 1, reagent selection-proportioning device 3 by watertight cable and Communication Control.Seawater sampling device 1 It is attached with reagent selection-proportioning device 3 with reagent with culture bag system 2 by peek hard tubes.
As shown in Fig. 2, seawater sampling device 1 mainly includes being mounted on the trepanning end cap 5 at the top of device, trepanning end cap 5 is pacified On cavity 15, the bottom of cavity 15 is equipped with end cover 16, and trepanning end cap 5, cavity 15 and end cover 16 pass through drawing Bar 14 is fixed.Pull rod 6 is installed, 6 lower end of pull rod connects mounting disc 7, is mounted with that seawater is compacted in mounting disc 7 on trepanning end cap 5 Dynamic pump 26 and tracer peristaltic pump 27.Solenoid valve 8,9,10,11,22,23,24 and 25 (wherein solenoid valves are mounted under mounting disc 7 25 are not shown in figure, spare as culture bag).Pull rod 12 is installed in 7 lower part of mounting disc, and mounting disc 13 passes through pull rod 12 and mounting disc 7 connections are fixed.Terminator peristaltic pump 21 and solenoid valve 17,18,19 and 20 are installed in mounting disc 13.
Reagent is mainly made of with culture bag system 2 reagent bag, is divided into cultivate reagent bag, tracer according to the difference of purposes Reagent bag and terminator reagent bag.
As shown in figure 3, reagent selection-proportioning device 3 mainly includes the trepanning end cap 28 on top, cavity 32 and lower seal End cap 33.Trepanning end cap 28, cavity 32 and lower seal end cap 33 are fixed by pull rod 30.28 lower part of trepanning end cap is installed by metal Pull rod 29 is fixed with intermediate mounting disc 31.It is fixedly mounted on disk 31 and rotary valve 34,35,36,37 and 38 is installed.
A kind of Biogeochemistry original position experimental provision for deep-sea can realize following 5 kinds of functions:Seawater is into culture Bag, reagent quantitative, reagent are into culture bag, the cleaning of reagent flow path, experiment terminates, terminator cleans.Its concrete operating principle such as Fig. 4 It is shown, under specific embodiment:
Seawater is into culture bag:Control device 4 sends electric signal to seawater sampling device 1, and seawater is compacted in seawater sampling device 1 Dynamic pump 26 and target culture bag corresponding channel solenoid valve are opened, and external seawater enters target under 26 swabbing action of seawater peristaltic pump Cultivate reagent bag.
Reagent quantitative:Control device 4 sends electric signal to seawater sampling device 1 and reagent selection-proportioning device 3 respectively. Tracer selection rotary valve 34 in reagent selection-proportioning device 3 gates target tracer reagent bag channel, and tracer quantitatively revolves Rotary valve 35 gates channel to waste discharge channel, and tracer peristaltic pump 27 is opened in seawater sampling device 1, by target tracer reagent Tracer is drawn into pipeline in bag, and the quantitative loop that tracer in pipeline is quantified to rotary valve 35 is full of.
Reagent is into culture bag:Control device 4 sends electric signal to seawater sampling device 1 and reagent selection-proportioning device 3. Tracer quantifies 35 channel of rotary valve and switches to tracer selection culture 36 access road of rotary valve in reagent selection-proportioning device 3, Channel is adjusted to target culture bag by tracer selection culture rotary valve 36, the seawater peristaltic pump 26 in seawater sample introduction control device 1 It is opened with tracer solenoid valve 8, under the swabbing action of seawater peristaltic pump 26, tracer is quantified 35 quantitative loop of rotary valve by seawater Interior quantitative tracer is carried along into target cultivate reagent bag.
Reagent flow path cleans:Control device 4 sends an electrical signal to seawater sampling device 1 and reagent selection-proportioning device 3. Tracer selection rotary valve 34 gates channel to seawater in reagent selection-proportioning device 3, and tracer quantifies rotary valve 35 will be logical Road is gated to waste discharge, and tracer peristaltic pump 27 is opened in seawater sampling device 1, and seawater fills under the suction of tracer peristaltic pump 27 Full entire flow path replaces the tracer filled in former pipeline.
Experiment terminates:Control device 4 sends an electrical signal to seawater sampling device 1 and reagent selection-proportioning device 3.Reagent Terminator quantifies rotary valve 37 and gates channel to terminator channel in selection-proportioning device 3, the end in seawater sampling device 1 Only agent peristaltic pump 21 is opened, and terminator is quantified to the quantitative loop of rotary valve 37 full of terminator.In reagent selection-proportioning device 3 Terminator, which quantifies rotary valve 37 and gates channel to terminating, selects culture 38 entrance of rotary valve, in reagent selection-proportioning device eventually Only selection culture rotary valve 38 gates channel to target culture bag, seawater peristaltic pump and terminator electricity in seawater sampling device 1 Magnet valve 9 is opened, and seawater enters flow path under the suction of seawater peristaltic pump 26, and terminator is quantified in 37 quantitative loop of rotary valve and is terminated Target culture bag is brought in agent into.
Terminator cleans:Control device 4 sends an electrical signal to seawater sampling device 1 and reagent selection-proportioning device 3.Examination Terminator quantifies rotary valve 37 and gates channel to the entrance for terminating selection culture rotary valve 38 in agent selection-proportioning device 3, whole Only selection culture rotary valve 38 gates channel to waste discharge, seawater peristaltic pump 26 and terminator solenoid valve 9 in seawater sampling device 1 It opens, seawater enters flow path under the suction of seawater peristaltic pump 26, and the terminator in pipeline is discharged by waste discharge mouth.
The utility model has the characteristics that:
1. the utility model can carry out quantitative sample injection, timing-quantitative sample injection of reagent and termination automatically in deep sea in-situ The functions such as experiment realize full-automatic Biochemistry Experiment in deep sea in-situ;
2. multiple comparison culture experiments can be achieved at the same time in the utility model.
Each embodiment is described by the way of progressive in this specification, the highlights of each of the examples are with other The difference of embodiment, just to refer each other for identical similar portion between each embodiment.
The foregoing description of the disclosed embodiments enables professional and technical personnel in the field to realize or new using this practicality Type.A variety of modifications of these embodiments will be apparent for those skilled in the art, determine herein The General Principle of justice can be realized in other embodiments without departing from the spirit or scope of the present utility model.Cause This, the utility model is not intended to be limited to the embodiments shown herein, and is to fit to and principles disclosed herein The most wide range consistent with features of novelty.

Claims (10)

1. a kind of Biogeochemistry original position experimental provision for deep-sea, which is characterized in that including:Seawater sampling device (1), Reagent and culture bag system (2), reagent selection-proportioning device (3) and control device (4);
The reagent includes cultivate reagent bag, tracer reagent bag and terminator reagent bag with culture bag system (2);
The control device (4) connects respectively with the seawater sampling device (1) and reagent selection-proportioning device (3) communication It connects;The control device (4) can send electric signal to the seawater sampling device (1) and reagent selection-proportioning device (3), make described in the successively entrance of seawater, the tracer in the tracer reagent bag and the terminator in the terminator reagent bag Cultivate reagent bag.
2. Biogeochemistry original position according to claim 1 experimental provision, which is characterized in that the seawater sampling device (1) including seawater housing and the seawater peristaltic pump (26) and solenoid valve that set within it;Seawater peristaltic pump (26) enter Mouthful can be contacted by the seawater housing with the external world, the solenoid valve be arranged on the seawater peristaltic pump (26) export with it is described Channel between cultivate reagent bag.
3. Biogeochemistry original position according to claim 2 experimental provision, which is characterized in that the cultivate reagent bag Quantity is multiple, and channel is both provided with the electromagnetism between each cultivate reagent bag and the seawater peristaltic pump (26) outlet Valve.
4. Biogeochemistry original position according to claim 2 experimental provision, which is characterized in that the seawater housing includes Cavity (15) and it is separately mounted to the trepanning end cap (5) at its both ends and end cover (16);The seawater peristaltic pump (26) Entrance can be contacted by the trepanning end cap (5) with the external world.
5. Biogeochemistry original position according to claim 2 experimental provision, which is characterized in that the reagent selection-fixed Amount device (3) quantifies rotary valve (35) including reagent housing and the tracer to set within it;The tracer quantitatively rotates Quantitative loop is provided in valve (35);
The seawater sampling device (1) further includes the tracer solenoid valve (8) being arranged in the seawater housing and tracer is compacted Dynamic pump (27);The entrance of the tracer peristaltic pump (27) is communicated in the tracer reagent bag;
The tracer quantifies rotary valve (35) and includes multiple channels:The tracer peristaltic pump is communicated in into tracer channel (27) outlet, waste discharge channel are communicated in waste fluid bag, and sea water passage is communicated in the outlet of the seawater peristaltic pump (26) and channel On be provided with the tracer solenoid valve (8), culture bag channel is communicated in the cultivate reagent bag.
6. Biogeochemistry original position according to claim 5 experimental provision, which is characterized in that the tracer reagent bag Quantity to be multiple;
The reagent selection-proportioning device (3) further includes the tracer selection rotary valve (34) being arranged in the reagent housing;
The tracer selection rotary valve (34) includes multiple channels:Multiple tracer selector channels one-to-one correspondence are communicated in multiple The tracer reagent bag, tracer channel are communicated in the entrance of the tracer peristaltic pump (27), can lead into sea water passage It crosses the reagent housing and is communicated in the external world.
7. Biogeochemistry original position according to claim 5 experimental provision, which is characterized in that the cultivate reagent bag Quantity is multiple;
The reagent selection-proportioning device (3) further includes the tracer selection culture rotary valve being arranged in the reagent housing (36);
The tracer selection culture rotary valve (36) includes multiple channels:Multiple culture bag selector channels one-to-one correspondence are communicated in more A cultivate reagent bag, tracer channel are communicated in the culture bag channel that the tracer quantifies rotary valve (35), and waste discharge is led to Road is communicated in waste fluid bag.
8. Biogeochemistry original position according to claim 2 experimental provision, which is characterized in that the reagent selection-fixed Amount device (3) quantifies rotary valve (37) including reagent housing and the terminator to set within it;The terminator quantitatively rotates Quantitative loop is provided in valve (37);
The seawater sampling device (1) further includes the terminator solenoid valve (9) being arranged in the seawater housing and terminator is compacted Dynamic pump (21);The outlet of the terminator peristaltic pump (21) is in the terminator reagent bag;
The terminator quantifies rotary valve (37) and includes multiple channels:Terminator channel is communicated in the terminator reagent bag, compacted Dynamic pump channel is communicated in the entrance of the terminator peristaltic pump (21), and sea water passage is communicated in going out for seawater peristaltic pump (26) The terminator solenoid valve (9) is provided on mouth and channel, culture bag channel is communicated in the cultivate reagent bag.
9. Biogeochemistry original position according to claim 8 experimental provision, which is characterized in that the cultivate reagent bag Quantity is multiple;
The reagent selection-proportioning device (3) further includes the termination selection culture rotary valve being arranged in the reagent housing (38);
The selection culture rotary valve (38) that terminates includes multiple channels:Multiple culture bag selector channels one-to-one correspondence are communicated in more A cultivate reagent bag, terminator channel are communicated in the culture bag channel that the terminator quantifies rotary valve (37), and waste discharge is led to Road is communicated in waste fluid bag.
10. the Biogeochemistry original position experimental provision according to claim 5-9 any one, which is characterized in that described Reagent housing includes cavity (32) and is separately mounted to the trepanning end cap (28) at its both ends and end cover (33).
CN201721742519.2U 2017-12-14 2017-12-14 A kind of Biogeochemistry original position experimental provision for deep-sea Expired - Fee Related CN207502550U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107907698A (en) * 2017-12-14 2018-04-13 中国科学院深海科学与工程研究所 A kind of Biogeochemistry original position experimental provision for deep-sea
CN109026629A (en) * 2018-06-26 2018-12-18 杭州电子科技大学 A kind of deep-sea peristaltic pump and the controllable long-term chemical reaction unit in deep-sea

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107907698A (en) * 2017-12-14 2018-04-13 中国科学院深海科学与工程研究所 A kind of Biogeochemistry original position experimental provision for deep-sea
CN109026629A (en) * 2018-06-26 2018-12-18 杭州电子科技大学 A kind of deep-sea peristaltic pump and the controllable long-term chemical reaction unit in deep-sea

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