CN107907698A - A kind of Biogeochemistry original position experimental provision for deep-sea - Google Patents
A kind of Biogeochemistry original position experimental provision for deep-sea Download PDFInfo
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- CN107907698A CN107907698A CN201711337178.5A CN201711337178A CN107907698A CN 107907698 A CN107907698 A CN 107907698A CN 201711337178 A CN201711337178 A CN 201711337178A CN 107907698 A CN107907698 A CN 107907698A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The invention discloses a kind of Biogeochemistry original position experimental provision for deep-sea, including seawater sampling device, reagent and culture bag system, reagent selection proportioning device and control device, following 5 kinds of functions can be realized by the effect of peristaltic pump, solenoid valve and rotary valve：Seawater is into culture bag, reagent quantitative, reagent is terminated into culture bag, the cleaning of reagent flow path, experiment and terminator cleaning.The existing indoor experimentation of experiment is placed seabed original position with method and is implemented by this programme, achievees the purpose that abyssopelagic organism geochemistry experiment device in deep sea in-situ timing, quantitative automatic progress sample culturing and labelling experiment.Biogeochemistry original position to be recycled experimental provision, take out culture bag therein, and in the changes of contents of experiment lab analysis reactant, reaction product and isotopic tracer, the experimental datas such as the Biogeochemistry dynamics of deep sea in-situ environment can be obtained, data supporting and foundation are provided to disclose marine biogeochemistry recycle recovery.
Description
Technical field
The present invention relates to Biogeochemistry culture experiment technical field, more particularly to a kind of biological earth for deep-sea
Chemical in situ experimental provision.
Background technology
With the reach of science, more and more problem in science focus on marine field, including Biogeochemistry
Etc. relevant field.
Current most experiments sample is to be carried the samples such as seawater from seabed by sampling modes such as sampler, device for fetching water
To sea, biochemistry culture and analysis are finally carried out in laboratory.
But in this process, sample has disengaged from original low temperature, high pressure living environment, discussion that the validity of sample has.
By the investigation of early period, rarely has the relevant report of the geochemical experimental provision in situ of similar abyssopelagic organism at present.
Therefore, with reference to the engineering demand and principle designing scheme of early period, a kind of experiment in situ of Biogeochemistry for deep-sea of research and development
Device, experiment porch is provided to explore the interaction mechanism between marine biogeochemistry.
The content of the invention
According to Biogeochemistry in-situ observation scientific research demand, the present invention proposes a kind of Biogeochemistry for deep-sea
Experimental provision in situ, can place seabed original position with method by the existing indoor experimentation of experiment and be implemented, reach deep-sea
Biogeochemistry experimental provision is in deep sea in-situ timing, the quantitative automatic purpose for carrying out sample culturing and labelling experiment.
To achieve the above object, the present invention provides following technical solution：
A kind of Biogeochemistry original position experimental provision for deep-sea, including：Seawater sampling device, reagent and culture bag
System, reagent selection-proportioning device and control device；
The reagent includes cultivate reagent bag, tracer reagent bag and terminator reagent bag with culture bag system；
The control device is connected with the seawater sampling device and reagent selection-proportioning device communication respectively；Institute
Electric signal can be sent to the seawater sampling device and reagent selection-proportioning device by stating control device, make seawater, described
Terminator in tracer and the terminator reagent bag in tracer reagent bag successively enters the cultivate reagent bag.
Preferably, the seawater sampling device includes seawater housing and the seawater peristaltic pump and electromagnetism that set within it
Valve；The entrance of the seawater peristaltic pump can be contacted by the seawater housing with the external world, and the solenoid valve is arranged on the sea
Passage between water wriggling pump discharge and the cultivate reagent bag.
Preferably, the quantity of the cultivate reagent bag is multiple each cultivate reagent bag and the seawater peristaltic pump
Passage is both provided with the solenoid valve between outlet.
Preferably, the seawater housing includes cavity and the perforate end cap and end cover that are separately mounted to its both ends；
The entrance of the seawater peristaltic pump can be contacted by the perforate end cap with the external world.
Preferably, the tracer that the reagent selection-proportioning device includes reagent housing and sets within it quantitatively revolves
Rotary valve；The tracer quantifies in rotary valve and is provided with quantitative loop；
The seawater sampling device further includes the tracer solenoid valve being arranged in the seawater housing and tracer is wriggled
Pump；The entrance of the tracer peristaltic pump is communicated in the tracer reagent bag；
The tracer, which quantifies rotary valve, includes multiple passages：The tracer peristaltic pump is communicated in into tracer passage
Outlet, waste discharge passage are communicated in waste fluid bag, and sea water passage is communicated on the outlet of the seawater peristaltic pump and passage and sets
Tracer solenoid valve is stated, culture bag passage is communicated in the cultivate reagent bag.
Preferably, the quantity of the tracer reagent bag is multiple；
Reagent selection-the proportioning device further includes the tracer selection rotary valve being arranged in the reagent housing；
The tracer selection rotary valve includes multiple passages：Multiple tracer selector channels one-to-one corresponding are communicated in multiple
The tracer reagent bag, tracer passage are communicated in the entrance of the tracer peristaltic pump, can pass through institute into sea water passage
State reagent housing and be communicated in the external world.
Preferably, the quantity of the cultivate reagent bag is multiple；
Reagent selection-the proportioning device further includes the tracer selection culture rotary valve being arranged in the reagent housing；
The tracer selection culture rotary valve includes multiple passages：Multiple culture bag selector channels one-to-one corresponding are communicated in more
A cultivate reagent bag, tracer passage are communicated in the culture bag passage that the tracer quantifies rotary valve, and waste discharge passage connects
Pass through waste fluid bag.
Preferably, the terminator that the reagent selection-proportioning device includes reagent housing and sets within it quantitatively revolves
Rotary valve；The terminator quantifies in rotary valve and is provided with quantitative loop；
The seawater sampling device further includes the terminator solenoid valve being arranged in the seawater housing and terminator is wriggled
Pump；The outlet of the terminator peristaltic pump is in the terminator reagent bag；
The terminator, which quantifies rotary valve, includes multiple passages：Terminator passage is communicated in the terminator reagent bag, compacted
Dynamic pump channel is communicated in the entrance of the terminator peristaltic pump, and sea water passage is communicated in the outlet of the seawater peristaltic pump and passage
On be provided with the terminator solenoid valve, culture bag passage is communicated in the cultivate reagent bag.
Preferably, the quantity of the cultivate reagent bag is multiple；
Reagent selection-the proportioning device further includes the termination selection culture rotary valve being arranged in the reagent housing；
The selection culture rotary valve that terminates includes multiple passages：Multiple culture bag selector channels one-to-one corresponding are communicated in more
A cultivate reagent bag, terminator passage are communicated in the culture bag passage that the terminator quantifies rotary valve, and waste discharge passage connects
Pass through waste fluid bag.
Preferably, the reagent housing includes cavity and the perforate end cap and end cover that are separately mounted to its both ends.
It can be seen from the above technical scheme that the Biogeochemistry experiment dress in situ provided by the present invention for deep-sea
Put, mainly utilize the process experiment data such as tracer method, the Biogeochemistry dynamics to obtain abyssal environment.In reality
During border uses, Biogeochemistry original position experimental provision can, timing automatic in deep sea in-situ, quantitatively by reactant and same position
Plain tracer is separately added into the seawater sample in target culture bag, with the life occurred automatically in tracer element mark in the original location environment
Thing geochemical reaction process (such as nitration reaction and anti-nitration reaction).Treat that biogeochemical reaction proceeds to certain process
Afterwards, Biogeochemistry original position experimental provision adds quantitative biogeochemical reaction terminator according to predetermined time course
(biostatic agent), to suppress the biogeochemical reaction in culture bag, reactant, reaction product and isotopic tracer are protected
There are in culture bag.Biogeochemistry original position to be recycled experimental provision, takes out culture bag therein, and in experiment lab analysis
The changes of contents of reactant, reaction product and isotopic tracer, can obtain the Biogeochemistry power of deep sea in-situ environment
Etc. experimental data, data supporting and foundation are provided to disclose marine biogeochemistry recycle recovery.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is attached drawing needed in technology description to be briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, without creative efforts, can be with
Other attached drawings are obtained according to these attached drawings.
Fig. 1 is the structure diagram of experimental provision provided in an embodiment of the present invention totality；
Fig. 2 is the structure diagram of seawater sampling device provided in an embodiment of the present invention；
Fig. 3 is the structure diagram of reagent selection-proportioning device provided in an embodiment of the present invention；
Fig. 4 is the work flow path principle sketch of experimental provision provided in an embodiment of the present invention.
Wherein, 1 is seawater sampling device, and 5 be perforate end cap, and 6 be pull rod, and 7 be mounting disc, and 8 be tracer solenoid valve, 9
For terminator solenoid valve, 10,11,17,18,19,20,22,23,24 and 25 between culture bag and seawater peristaltic pump passage electricity
Magnet valve, 12 be pull rod, and 13 be mounting disc, and 14 be pull rod, and 15 be cavity, and 16 be end cover, and 21 be terminator peristaltic pump, and 26 are
Seawater peristaltic pump, 27 be tracer peristaltic pump；
2 be reagent and culture bag system；
3 be reagent selection-proportioning device, and 28 be perforate end cap, and 29 be pull rod, and 30 be pull rod, and 31 be mounting disc, and 32 be chamber
Body, 33 be end cover, and 34 select rotary valve for tracer, and 35 quantify rotary valve for tracer, and 36 be tracer selection culture rotation
Rotary valve, 37 quantify rotary valve for terminator, and 38 cultivate rotary valve to terminate selection；
4 devices in order to control.
Embodiment
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other without making creative work
Embodiment, belongs to the scope of protection of the invention.
Biogeochemistry original position experimental provision provided in an embodiment of the present invention for deep-sea, its core improvement exist
In, including：Seawater sampling device 1, reagent and culture bag system 2, reagent selection-proportioning device 3 and control device 4, its structure
It is referred to shown in Fig. 1；
Reagent includes cultivate reagent bag, tracer reagent bag and terminator reagent bag with culture bag system 2；
Control device 4 is connected with seawater sampling device 1 and reagent selection-communication of proportioning device 3 respectively；4 energy of control device
Electric signal is enough sent to seawater sampling device 1 and reagent selection-proportioning device 3, makes seawater, the tracer in tracer reagent bag
Successively enter cultivate reagent bag with the terminator in terminator reagent bag.
It can be seen from the above technical scheme that the Biogeochemistry provided in an embodiment of the present invention for deep-sea is in situ
Experimental provision, can carry out the functions such as quantitative sample injection, timing-quantitative sample injection of reagent and termination experiment automatically in deep sea in-situ,
Full-automatic Biochemistry Experiment is realized in deep sea in-situ.
Preferably, seawater sampling device 1 includes seawater housing and the seawater peristaltic pump 26 and electromagnetism that set within it
Valve；There is seawater housing pressure-resistance structure can provide protection for wherein component；The entrance of seawater peristaltic pump 26 can pass through seawater
Housing is contacted with the external world, and solenoid valve is arranged on seawater peristaltic pump 26 and exports passage between cultivate reagent bag.Using peristaltic pump
Compared with other sample introduction mechanisms, has the following advantages：Do not contacted with fluid media (medium), so that avoid influencing to test, while flow velocity is steady
It is fixed, adapt to hyperbaric environment work.
Further, the quantity of cultivate reagent bag is multiple, between each cultivate reagent bag and seawater peristaltic pump 26 outlet
Passage is both provided with solenoid valve, its structure is referred to shown in Fig. 2 and Fig. 4.So set, multiple contrast trainings can be realized at the same time
Support experiment.When needing seawater into culture bag, control device 4 sends electric signal to seawater sampling device 1, seawater sampling device 1
Maritime interior waters peristaltic pump 26 and target culture bag respective channel solenoid valve are opened, and exterior seawater is under 26 swabbing action of seawater peristaltic pump
Into target cultivate reagent bag.
Specifically, seawater housing includes cavity 15 and the perforate end cap 5 and end cover 16 that are separately mounted to its both ends；
The entrance of seawater peristaltic pump 26 can be contacted by perforate end cap 5 with the external world.Its structure is referred to shown in Fig. 2, seawater sample introduction
Device 1 mainly includes being installed on the perforate end cap 5 at the top of device, and perforate end cap 5 is installed on cavity 15, the bottom peace of cavity 15
Equipped with end cover 16, perforate end cap 5, cavity 15 and end cover 16 are fixed by pull rod 14；Installed on perforate end cap 5
Pull rod 6,6 lower end of pull rod connect mounting disc 7, peristaltic pump 22 and peristaltic pump 23 are mounted with mounting disc 7；It is mounted with mounting disc 7
Solenoid valve 8,9,10,11,22,23,24 and 25；Pull rod 12 is installed in 7 lower part of mounting disc, and mounting disc 13 passes through pull rod 12 and mounting disc
7 connections are fixed；Peristaltic pump 21 and solenoid valve 17,18,19,20 are installed, above-mentioned arrangement is compact-sized in mounting disc 13.
Can be by controlling the amount of tracer in tracer reagent bag to be quantitatively adding to realize.Preferably implement in this programme
In example, the tracer that reagent selection-proportioning device 3 includes reagent housing and sets within it quantifies rotary valve 35；The tracer
Agent quantifies in rotary valve 35 and is provided with quantitative loop between entrance and exit, and tracer is more precisely quantitatively adding to realize；
Seawater sampling device 1 further includes the tracer solenoid valve 8 and tracer peristaltic pump 27 being arranged in seawater housing；Show
The entrance of track agent peristaltic pump 27 is communicated in tracer reagent bag；
Tracer, which quantifies rotary valve 35, includes multiple passages：Going out for tracer peristaltic pump 27 is communicated in into tracer passage
Mouthful, waste discharge passage is communicated in waste fluid bag (avoiding influencing environment with recycling), and sea water passage is communicated in the outlet of seawater peristaltic pump 26
And tracer solenoid valve 8 is provided with passage, culture bag passage is communicated in cultivate reagent bag.Cut using rotary valve and other pipelines
Change structure to compare, have the advantages that small, integrated level is high and low in energy consumption；
By said structure, when needing reagent quantitative function, control device 4 sends electric signal to seawater sample introduction and fills respectively
Put 1 and reagent selection-proportioning device 3；Tracer in reagent selection-proportioning device 3 quantifies rotary valve 35 and selects exit passageway
Pass to waste discharge passage, access road is gated into tracer passage, and tracer peristaltic pump 27 is opened in seawater sampling device 1, will
Tracer is drawn into pipeline in tracer reagent bag, and the quantitative loop of rotary valve in pipeline 35 is full of；
When needing reagent into culture bag function, control device 4 sends electric signal to seawater sampling device 1 and reagent and selects
Select-proportioning device 3；Tracer in reagent selection-proportioning device 3 quantifies 35 exit passageway of rotary valve and switches to cultivate reagent
Bag, access road switch to sea water passage, and the seawater peristaltic pump 26 and tracer solenoid valve 8 in seawater sample introduction control device 1 are opened
Open, under the swabbing action of seawater peristaltic pump 26, tracer is quantified the quantitative tracer in 35 quantitative loop of rotary valve and taken by seawater
Band enters cultivate reagent bag；
When needing reagent flow path cleaning function, control device 4 sends electric signal to seawater sampling device 1 and reagent and selects
Select-proportioning device 3；Tracer in reagent selection-proportioning device 3, which quantifies rotary valve 35 and gates exit passageway to waste discharge, to be led to
Road, access road are gated into tracer passage, and tracer peristaltic pump 27 is opened in seawater sampling device 1, is wriggled in tracer
Seawater is full of whole flow path under the suction of pump 27, the tracer filled in former pipeline is replaced, so as to carry out other experiments again.
Further, to be multiple, its structure is referred to shown in Fig. 4 the quantity of tracer reagent bag, from meeting different realities
The needs tested；
Reagent selection-proportioning device 3 further includes the tracer selection rotary valve 34 being arranged in reagent housing；
Tracer selection rotary valve 34 includes multiple passages：Multiple tracer selector channels one-to-one corresponding are communicated in multiple
Tracer reagent bag, tracer passage are communicated in the entrance of tracer peristaltic pump 27, can pass through reagent housing into sea water passage
It is communicated in the external world.I.e. the entrance of tracer peristaltic pump 27 selects rotary valve 34 to be communicated in each tracer reagent bag by tracer.
When needing reagent quantitative function, reagent gates target tracer reagent bag passage, outlet gating tracer into 34 entrance of rotary valve
Agent passage, so that tracer in target tracer reagent bag is drawn into pipeline；When needing reagent flow path cleaning function, examination
Access road is gated into sea water passage, outlet and gated to tracer passage by agent into rotary valve 34.
Specifically, the quantity of cultivate reagent bag is multiple, its structure is referred to shown in Fig. 4, can be realized at the same time multiple
Contrast culture experiment；
Reagent selection-proportioning device 3 further includes the tracer selection culture rotary valve 36 being arranged in reagent housing；Tracer is selected
Selecting culture rotary valve 36 includes multiple passages：Multiple culture bag selector channels correspond and are communicated in multiple cultivate reagent bags, show
Track agent passage is communicated in tracer and quantifies rotary valve 35, and waste discharge passage is communicated in waste fluid bag (avoiding influencing environment with recycling).I.e.
Tracer quantifies rotary valve 35 and selects culture rotary valve 36 to be communicated in each cultivate reagent bag by tracer.Reagent is being needed into culture
During bag function, exit passageway is adjusted to target culture bag selector channel by tracer selection culture rotary valve 36, access road select to
Tracer passage, so that quantitative tracer is carried along into target cultivate reagent bag by seawater.
Can be by controlling the amount of terminator in terminator reagent bag to be quantitatively adding to realize.Preferably implement in this programme
In example, the terminator that reagent selection-proportioning device 3 includes reagent housing and sets within it quantifies rotary valve 37；The termination
Agent quantifies in rotary valve 37 and is provided with quantitative loop between entrance and exit, and terminator is more precisely quantitatively adding to realize；
Seawater sampling device 1 further includes the terminator solenoid valve 9 and terminator peristaltic pump 21 being arranged in seawater housing；Eventually
Only the outlet of agent peristaltic pump 21 is in terminator reagent bag；
Terminator, which quantifies rotary valve 37, includes multiple passages：Terminator passage is communicated in terminator reagent bag, and peristaltic pump leads to
Road is communicated in the entrance of terminator peristaltic pump 21, and sea water passage is communicated on the outlet of seawater peristaltic pump 26 and passage and is provided with end
Only agent solenoid valve 9, culture bag passage are communicated in cultivate reagent bag.
By said structure, when needing to test expiry feature, control device 4 sends an electrical signal to seawater sampling device 1
With reagent selection-proportioning device 3；Terminator quantifies rotary valve 37 and gates access road to end in reagent selection-proportioning device 3
Only agent passage, exit passageway is gated to wriggling pump channel, and the terminator peristaltic pump 21 in seawater sampling device 1 is opened, will be eventually
Only agent quantifies the quantitative loop of rotary valve 37 full of terminator；Then, terminator quantifies rotary valve 37 and gates exit passageway to training
Bag passage is supported, access road is gated to sea water passage, seawater peristaltic pump 26 and terminator solenoid valve 9 and is opened, is wriggled in seawater
Seawater enters flow path under the suction of pump 26, and terminator is quantified terminator in 37 quantitative loop of rotary valve brings culture bag into.
Further, the quantity of cultivate reagent bag is multiple that its structure is referred to shown in Fig. 4, can be realized at the same time more
A contrast culture experiment；
Reagent selection-proportioning device 3 further includes the termination selection culture rotary valve 38 being arranged in reagent housing；Terminate choosing
Selecting culture rotary valve 38 includes multiple passages：Multiple culture bag selector channels correspond and are communicated in multiple cultivate reagent bags, eventually
Only agent passage is communicated in the culture bag passage that terminator quantifies rotary valve 37, and waste discharge passage is communicated in waste fluid bag and (is avoided with recycling
Influence environment).I.e. terminator quantifies rotary valve 37 and is communicated in each cultivate reagent bag by terminating selection culture rotary valve 38.
So set, when needing terminator cleaning function, control device 4 sends an electrical signal to 1 He of seawater sampling device
Reagent selection-proportioning device 3；Terminator quantifies rotary valve 37 and gates exit passageway to culture in reagent selection-proportioning device 3
Bag passage, access road is gated to sea water passage, is terminated selection culture rotary valve 38 and is gated exit passageway to waste discharge passage,
Access road is gated to terminator passage, 1 maritime interior waters peristaltic pump 26 of seawater sampling device and terminator solenoid valve 9 and is opened,
Seawater enters flow path under the suction of seawater peristaltic pump 26, and the terminator in pipeline is discharged by waste discharge mouth
Specifically, reagent housing includes cavity 32 and the perforate end cap 28 and end cover that are separately mounted to its both ends
33, its structure is referred to shown in Fig. 3.
This programme is described further with reference to specific embodiment：
As shown in Figure 1, a kind of Biogeochemistry original position experimental provision for deep-sea, including four parts, it is respectively
Seawater sampling device 1, reagent and culture bag system 2, reagent selection-proportioning device 3 and control device 4.Control device 4 and seawater
It is powered between sampling device 1, reagent selection-proportioning device 3 by watertight cable and Communication Control.Seawater sampling device 1
It is attached with reagent selection-proportioning device 3 with reagent with culture bag system 2 by peek hard tubes.
As shown in Fig. 2, seawater sampling device 1 mainly includes being installed on the perforate end cap 5 at the top of device, perforate end cap 5 is pacified
On cavity 15, the bottom of cavity 15 is provided with end cover 16, and perforate end cap 5, cavity 15 and end cover 16 pass through drawing
Bar 14 is fixed.Pull rod 6 is installed, 6 lower end of pull rod connects mounting disc 7, is mounted with that seawater is compacted in mounting disc 7 on perforate end cap 5
Dynamic pump 26 and tracer peristaltic pump 27.Solenoid valve 8,9,10,11,22,23,24 and 25 (wherein solenoid valves are mounted with mounting disc 7
It is spare as culture bag not shown in 25 figures).Pull rod 12 is installed in 7 lower part of mounting disc, and mounting disc 13 passes through pull rod 12 and mounting disc
7 connections are fixed.Terminator peristaltic pump 21 and solenoid valve 17,18,19 and 20 are installed in mounting disc 13.
Reagent is mainly made of with culture bag system 2 reagent bag, is divided into cultivate reagent bag, tracer according to the difference of purposes
Reagent bag and terminator reagent bag.
As shown in figure 3, reagent selection-proportioning device 3 mainly includes the perforate end cap 28 on top, cavity 32 and lower seal
End cap 33.Perforate end cap 28, cavity 32 and lower seal end cap 33 are fixed by pull rod 30.28 lower part of perforate end cap is installed by metal
Pull rod 29 is fixed with middle mounting disc 31.It is fixedly mounted on disk 31 and rotary valve 34,35,36,37 and 38 is installed.
A kind of Biogeochemistry original position experimental provision for deep-sea can realize following 5 kinds of functions：Seawater is into culture
Bag, reagent quantitative, reagent are into culture bag, the cleaning of reagent flow path, experiment terminates, terminator cleans.Its concrete operating principle such as Fig. 4
It is shown, under specific embodiment：
Seawater is into culture bag：It is compacted to seawater sampling device 1,1 maritime interior waters of seawater sampling device that control device 4 sends electric signal
Dynamic pump 26 and target culture bag respective channel solenoid valve are opened, and exterior seawater enters target under 26 swabbing action of seawater peristaltic pump
Cultivate reagent bag.
Reagent quantitative：Control device 4 sends electric signal to seawater sampling device 1 and reagent selection-proportioning device 3 respectively.
Tracer selection rotary valve 34 in reagent selection-proportioning device 3 gates target tracer reagent bag passage, and tracer quantitatively revolves
Rotary valve 35 gates passage to waste discharge passage, and tracer peristaltic pump 27 is opened in seawater sampling device 1, by target tracer reagent
Tracer is drawn into pipeline in bag, and the quantitative loop that tracer in pipeline is quantified to rotary valve 35 is full of.
Reagent is into culture bag：Control device 4 sends electric signal to seawater sampling device 1 and reagent selection-proportioning device 3.
Tracer quantifies 35 passage of rotary valve and switches to tracer selection culture 36 access road of rotary valve in reagent selection-proportioning device 3,
Passage is adjusted to target culture bag by tracer selection culture rotary valve 36, the seawater peristaltic pump 26 in seawater sample introduction control device 1
Opened with tracer solenoid valve 8, under the swabbing action of seawater peristaltic pump 26, tracer is quantified 35 quantitative loop of rotary valve by seawater
Interior quantitative tracer is carried along into target cultivate reagent bag.
Reagent flow path cleans：Control device 4 sends an electrical signal to seawater sampling device 1 and reagent selection-proportioning device 3.
Tracer selection rotary valve 34 gates passage to seawater in reagent selection-proportioning device 3, and tracer quantifies rotary valve 35 will be logical
Road is gated to waste discharge, and tracer peristaltic pump 27 is opened in seawater sampling device 1, and seawater fills under the suction of tracer peristaltic pump 27
Full whole flow path, replaces the tracer filled in former pipeline.
Experiment terminates：Control device 4 sends an electrical signal to seawater sampling device 1 and reagent selection-proportioning device 3.Reagent
Terminator quantifies rotary valve 37 and gates passage to terminator passage in selection-proportioning device 3, the end in seawater sampling device 1
Only agent peristaltic pump 21 is opened, and terminator is quantified to the quantitative loop of rotary valve 37 full of terminator.In reagent selection-proportioning device 3
Terminator, which quantifies rotary valve 37 and gates passage to terminating, selects culture 38 entrance of rotary valve, in reagent selection-proportioning device eventually
Only selection culture rotary valve 38 gates passage to target culture bag, 1 maritime interior waters peristaltic pump of seawater sampling device and terminator electricity
Magnet valve 9 is opened, and seawater enters flow path under the suction of seawater peristaltic pump 26, and terminator is quantified in 37 quantitative loop of rotary valve and is terminated
Target culture bag is brought in agent into.
Terminator cleans：Control device 4 sends an electrical signal to seawater sampling device 1 and reagent selection-proportioning device 3.Examination
Terminator quantifies rotary valve 37 and gates passage to the entrance for terminating selection culture rotary valve 38 in agent selection-proportioning device 3, whole
Only selection culture rotary valve 38 gates passage to waste discharge, 1 maritime interior waters peristaltic pump 26 of seawater sampling device and terminator solenoid valve 9
Open, seawater enters flow path under the suction of seawater peristaltic pump 26, and the terminator in pipeline is discharged by waste discharge mouth.
The invention has the characteristics that：
1. the present invention can carry out quantitative sample injection, timing-quantitative sample injection of reagent and termination experiment automatically in deep sea in-situ
Etc. function, full-automatic Biochemistry Experiment is realized in deep sea in-situ；
2. multiple contrast culture experiments can be achieved at the same time in the present invention.
Each embodiment is described by the way of progressive in this specification, what each embodiment stressed be and other
The difference of embodiment, between each embodiment identical similar portion mutually referring to.
The foregoing description of the disclosed embodiments, enables professional and technical personnel in the field to realize or use the present invention.
A variety of modifications to these embodiments will be apparent for those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, it is of the invention
The embodiments shown herein is not intended to be limited to, and is to fit to and the principles and novel features disclosed herein phase one
The most wide scope caused.
Claims (10)
- A kind of 1. Biogeochemistry original position experimental provision for deep-sea, it is characterised in that including：Seawater sampling device (1), Reagent and culture bag system (2), reagent selection-proportioning device (3) and control device (4)；The reagent includes cultivate reagent bag, tracer reagent bag and terminator reagent bag with culture bag system (2)；The control device (4) connects with the seawater sampling device (1) and reagent selection-proportioning device (3) communication respectively Connect；The control device (4) can send electric signal to the seawater sampling device (1) and reagent selection-proportioning device (3), make described in the successively entrance of seawater, the tracer in the tracer reagent bag and the terminator in the terminator reagent bag Cultivate reagent bag.
- 2. Biogeochemistry original position according to claim 1 experimental provision, it is characterised in that the seawater sampling device (1) seawater housing and the seawater peristaltic pump (26) and solenoid valve that set within it are included；Seawater peristaltic pump (26) enter Mouthful can be contacted by the seawater housing with the external world, the solenoid valve be arranged on the seawater peristaltic pump (26) export with it is described Passage between cultivate reagent bag.
- 3. Biogeochemistry original position according to claim 2 experimental provision, it is characterised in that the cultivate reagent bag Quantity is multiple, and passage is both provided with the electromagnetism between each cultivate reagent bag and the seawater peristaltic pump (26) outlet Valve.
- 4. Biogeochemistry original position according to claim 2 experimental provision, it is characterised in that the seawater housing includes Cavity (15) and the perforate end cap (5) and end cover (16) for being separately mounted to its both ends；The seawater peristaltic pump (26) Entrance can be contacted by the perforate end cap (5) with the external world.
- 5. Biogeochemistry original position according to claim 2 experimental provision, it is characterised in that the reagent selection-fixed The tracer that amount device (3) includes reagent housing and sets within it quantifies rotary valve (35)；The tracer quantitatively rotates Quantitative loop is provided with valve (35)；The seawater sampling device (1) further includes the tracer solenoid valve (8) being arranged in the seawater housing and tracer is compacted Dynamic pump (27)；The entrance of the tracer peristaltic pump (27) is communicated in the tracer reagent bag；The tracer, which quantifies rotary valve (35), includes multiple passages：The tracer peristaltic pump is communicated in into tracer passage (27) outlet, waste discharge passage are communicated in waste fluid bag, and sea water passage is communicated in the outlet of the seawater peristaltic pump (26) and passage On be provided with the tracer solenoid valve (8), culture bag passage is communicated in the cultivate reagent bag.
- 6. Biogeochemistry original position according to claim 5 experimental provision, it is characterised in that the tracer reagent bag Quantity to be multiple；The reagent selection-proportioning device (3) further includes the tracer selection rotary valve (34) being arranged in the reagent housing；The tracer selection rotary valve (34) includes multiple passages：Multiple tracer selector channels one-to-one corresponding are communicated in multiple The tracer reagent bag, tracer passage are communicated in the entrance of the tracer peristaltic pump (27), can lead into sea water passage Cross the reagent housing and be communicated in the external world.
- 7. Biogeochemistry original position according to claim 5 experimental provision, it is characterised in that the cultivate reagent bag Quantity is multiple；The reagent selection-proportioning device (3) further includes the tracer selection culture rotary valve being arranged in the reagent housing (36)；The tracer selection culture rotary valve (36) includes multiple passages：Multiple culture bag selector channels one-to-one corresponding are communicated in more A cultivate reagent bag, tracer passage are communicated in the culture bag passage that the tracer quantifies rotary valve (35), and waste discharge is led to Road is communicated in waste fluid bag.
- 8. Biogeochemistry original position according to claim 2 experimental provision, it is characterised in that the reagent selection-fixed The terminator that amount device (3) includes reagent housing and sets within it quantifies rotary valve (37)；The terminator quantitatively rotates Quantitative loop is provided with valve (37)；The seawater sampling device (1) further includes the terminator solenoid valve (9) being arranged in the seawater housing and terminator is compacted Dynamic pump (21)；The outlet of the terminator peristaltic pump (21) is in the terminator reagent bag；The terminator, which quantifies rotary valve (37), includes multiple passages：Terminator passage is communicated in the terminator reagent bag, compacted Dynamic pump channel is communicated in the entrance of the terminator peristaltic pump (21), and sea water passage is communicated in going out for seawater peristaltic pump (26) The terminator solenoid valve (9) is provided with mouth and passage, culture bag passage is communicated in the cultivate reagent bag.
- 9. Biogeochemistry original position according to claim 8 experimental provision, it is characterised in that the cultivate reagent bag Quantity is multiple；The reagent selection-proportioning device (3) further includes the termination selection culture rotary valve being arranged in the reagent housing (38)；The selection culture rotary valve (38) that terminates includes multiple passages：Multiple culture bag selector channels one-to-one corresponding are communicated in more A cultivate reagent bag, terminator passage are communicated in the culture bag passage that the terminator quantifies rotary valve (37), and waste discharge is led to Road is communicated in waste fluid bag.
- 10. the Biogeochemistry original position experimental provision according to claim 5-9 any one, it is characterised in that described Reagent housing includes cavity (32) and the perforate end cap (28) and end cover (33) that are separately mounted to its both ends.
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