CN101984039A - Experiment incubator capable of measuring depositional physicochemical parameters - Google Patents

Experiment incubator capable of measuring depositional physicochemical parameters Download PDF

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Publication number
CN101984039A
CN101984039A CN201010522057XA CN201010522057A CN101984039A CN 101984039 A CN101984039 A CN 101984039A CN 201010522057X A CN201010522057X A CN 201010522057XA CN 201010522057 A CN201010522057 A CN 201010522057A CN 101984039 A CN101984039 A CN 101984039A
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settling
culture tube
physical
measuring
magnetic stirring
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CN101984039B (en
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周阳靖
徐继荣
马晓磊
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Ningbo University
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Ningbo University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/46—Means for fastening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

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Abstract

The invention discloses an experiment incubator capable of measuring the depositional physicochemical parameters. The experiment incubator contains a box body. The experiment incubator is characterized in that the inside of the box body is provided with a plurality of sediment culture tubes; the upper and lower ends of each sediment culture tube are separately provided with a sealing plug, a liquid inlet pipe and a liquid outlet pipe penetrate through each sealing plug on the upper end, the lower end of each liquid outlet pipe extends in the culture solution of one sediment culture tube, the liquid outlet pipe of the sediment culture tube is communicated with the liquid inlet pipe of an adjacent sediment culture tube through a connecting duct; one connecting duct is provided with a miniature circulating pump used for driving the culture solution to flow circularly; and at least one sediment culture tube is provided with a multi-parameter electrode used for measuring the depositional physicochemical parameters. The advantage of the experiment incubator is as follows: the experiment incubator can be used for thermostatic culture and experimental measurement, thus the related scientific experiment and data monitoring can be performed and the environmental monitoring and scientific research can be facilitated.

Description

A kind of experiment incubator of measuring the settling physical and chemical parameter
Technical field
The present invention relates to a kind of experiment incubator, especially relate to a kind of experiment incubator of measuring the settling physical and chemical parameter.
Background technology
Worldwide the pollution of river, lake and coastal ocean is the focus environmental problem that society is paid close attention at present, people focus on eye on the eutrophication and pollution of water body, pollution aggravation along with water body, settling becomes the home to return to of pollutent, and enter sedimental pollution substance under action of microorganisms, the secondary pollution that release causes is the realistic problem that can not be ignored, and wherein the biomass geochemistry round-robin of the oxygen consumption of pollution substance and nitrogen is measured and the too busy to get away experiment of research incubator in the settling.
The current experiments incubator generally comprises casing, during use settling to be measured is put into casing and cultivates, and take out the mensuration that settling carries out physical and chemical parameter behind the cultivation certain hour, but this experiment incubator can not be cultivated simultaneously and measuring.
Summary of the invention
Technical problem to be solved by this invention provides a kind ofly can cultivate with measuring simultaneously and can measure the experiment incubator of settling physical and chemical parameter.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: a kind of experiment incubator of measuring the settling physical and chemical parameter, comprise casing, be provided with a plurality of settling culture tubes in the described casing, the two ends up and down of described settling culture tube are respectively arranged with sealing plug, run through on the described sealing plug on being in and be provided with liquid-inlet pipe and drain pipe, the lower ending opening of described drain pipe stretches in the nutrient solution of described settling culture tube, the drain pipe of described settling culture tube is connected by linking conduit with the liquid-inlet pipe of adjacent described settling culture tube, one of them described linking conduit is provided with and is used to drive the small-sized recycle pump that described nutrient solution circulates, and is provided with the multiparameter electrode that is used to measure the settling physical and chemical parameter at least one described settling culture tube.
Also be provided with whipping appts in the described casing, described whipping appts comprises micromachine, active magnetic stirring rotator and driven magnetic stirring rotator, described active magnetic stirring rotator is positioned at the intravital region intermediate of described case, described settling culture tube is evenly distributed in the described casing around described active magnetic stirring rotator is rounded, and described driven magnetic stirring rotator is positioned at described settling culture tube.
The stirring velocity of described active magnetic stirring rotator and described driven magnetic stirring rotator is 30r/min-180r/min.
Described active magnetic stirring rotator and described driven magnetic stirring rotator adopt ndfeb magnetic material to manufacture.
Also be provided with the constent temperature heater that is used to heat the water that fills in the casing in the described casing, described constent temperature heater is connected with the outer radiator valve that is provided with of described casing.
The lower ending opening of described liquid-inlet pipe stretches in the nutrient solution of described settling culture tube, and the degree of depth that described liquid-inlet pipe vertically stretches into nutrient solution vertically stretches into the degree of depth of nutrient solution greater than described drain pipe.
Described settling physical and chemical parameter comprises pH, Eh, DO or NH 3-N.
Described casing adopts transparent organic glass to manufacture, and described settling culture tube adopts transparent organic glass or polycarbonate material to manufacture.
Compared with prior art, the invention has the advantages that: owing to be provided with a plurality of settling culture tubes in the casing, by adjusting small-sized recycle pump the nutrient solution in each settling culture tube is exchanged mutually, keep each settling culture tube culture condition unanimity, by sedimental physical and chemical parameter pH, Eh, DO, NH in the multiparameter determination of electrode culturing process 3The variation of-N, mensuration can experimentize when cultivating.
Owing to also be provided with whipping appts in the casing, by the initiatively rotation of magnetic stirring rotator of micromachine control, rotate synchronously thereby drive driven magnetic stirring rotator, realized that all even settling culture tube of bath temperature material spreads uniform dual function in the casing by the interaction force between magnetic; Owing to also be provided with the constent temperature heater that is used to heat water temperature in the casing in the casing, constent temperature heater is connected with casing radiator valve outward, and culture temperature is carried out under controlled condition.
Because the lower ending opening of liquid-inlet pipe also stretches in the nutrient solution of settling culture tube, the degree of depth that liquid-inlet pipe vertically stretches into nutrient solution vertically stretches into the degree of depth of nutrient solution greater than drain pipe, therefore nutrient solution of sending into from other settling culture tube and the settling below that enters the settling culture tube, thus the nutrient solution and the settling that more help in the settling culture tube circulate mutual exchange up and down.
Because casing adopts transparent organic glass to manufacture, the settling culture tube adopts transparent organic glass or polycarbonate material to manufacture, and helps the experimenter and observes.
Therefore, a kind of experiment incubator of measuring the settling physical and chemical parameter of the present invention in constant temperature culture, the mensuration that can experimentize, the scientific experiment that can be correlated with and data monitoring help environmental monitoring and scientific research.
Description of drawings
Fig. 1 is a structural representation of the present invention;
Fig. 2 is the structural representation of settling culture tube of the present invention.
Embodiment
Embodiment describes in further detail the present invention below in conjunction with accompanying drawing.
A kind of experiment incubator of measuring the settling physical and chemical parameter, as Fig. 1, shown in Figure 2, comprise casing 1, be provided with a plurality of settling culture tubes 2 in this casing 1, the two ends up and down of described settling culture tube 2 are respectively arranged with sealing plug 9, run through on the sealing plug 9 on being in and be provided with liquid-inlet pipe 10 and drain pipe 11, the lower ending opening of drain pipe 11 stretches in the nutrient solution of settling culture tube 2, the drain pipe 11 of settling culture tube 2 is connected by linking conduit 5 with the liquid-inlet pipe 10 of adjacent settling culture tube 2, one of them linking conduit 5 is provided with and is used to drive the small-sized recycle pump 3 that nutrient solution circulates, thereby closed cycle pipeline of whole formation, by adjusting small-sized recycle pump the nutrient solution in each settling culture tube is exchanged mutually, keep each settling culture tube culture condition unanimity, be provided with the multiparameter electrode 6 that is used to measure settling 13 physical and chemical parameters in one or more settling culture tubes 2 simultaneously.
In this specific embodiment, also be provided with whipping appts in the casing 1, whipping appts comprises micromachine 4, active magnetic stirring rotator 8 and driven magnetic stirring rotator 12, initiatively magnetic stirring rotator 8 is positioned at the region intermediate of casing 1, settling culture tube 2 is around active magnetic stirring rotator 8 rounded being evenly distributed in the casing, driven magnetic stirring rotator 12 is positioned at settling culture tube 2, by the initiatively rotation of magnetic stirring rotator of micromachine control, thereby driving driven magnetic stirring rotator by the interaction force between magnetic rotates synchronously, be used to realize that all even settling culture tube of bath temperature material spreads uniform dual function in the casing, wherein the stirring velocity of micromachine 4 control active magnetic stirring rotators 8 and driven magnetic stirring rotator 12 is 30r/min-180r/min, and initiatively magnetic stirring rotator and driven magnetic stirring rotator adopt ndfeb magnetic material to manufacture.
In this specific embodiment, also be provided with the constent temperature heater 7 that is used to heat the water that fills in the casing 1 in the casing 1, constent temperature heater 7 is connected with casing 1 radiator valve outward, comes control condition that culture temperature is carried out under controlled condition.
In this specific embodiment, the lower ending opening of liquid-inlet pipe also stretches in the nutrient solution of settling culture tube, the degree of depth that liquid-inlet pipe vertically stretches into nutrient solution vertically stretches into the degree of depth of nutrient solution greater than drain pipe, therefore nutrient solution of sending into from other settling culture tube and the settling below that enters the settling culture tube, thus the nutrient solution and the settling that more help in the settling culture tube circulate mutual exchange up and down.
In this specific embodiment, the settling physical and chemical parameter comprises pH value, Eh(redox potential), the DO(dissolved oxygen) or NH 3-N(ammonia-state nitrogen), especially for DO(dissolved oxygen in the settling 13) or NH 3-N(ammonia-state nitrogen) measuring, need settling 13 to cultivate in confined conditions, need to avoid entrained air in the settling simultaneously, therefore settling culture tube 2 is inserted in the settling that needs to measure, treat that settling partly enters settling culture tube 2, cover the sealing plug 9 of lower end and the sealing plug 9 of upper end again, thereby guaranteed that settling 13 cultivates in confined conditions.
In this specific embodiment, casing 1 adopts transparent organic glass to manufacture, settling culture tube 2 adopts transparent organic glass or polycarbonate material to manufacture, and is convenient to experimentation is carried out Real Time Observation, can also take a sample when cultivating or other material of interpolation in settling culture tube 2.
Connect the constent temperature heater power supply during use, when bath temperature reaches design temperature, open small-sized recycle pump, whipping appts and multiparameter determination of electrode device, variation according to each physical and chemical parameter in the requirement of experiment record culturing process, scientific experiment of being correlated with and data monitoring, cultivate sampling midway if desired and can disconnect linking conduit, open the sealing plug sampling or add other material, the volume of experiment incubator of the present invention and the quantity of settling culture tube can increase or reduce as required simultaneously.

Claims (8)

1. the experiment incubator that can measure the settling physical and chemical parameter, comprise casing, it is characterized in that: be provided with a plurality of settling culture tubes in the described casing, the two ends up and down of described settling culture tube are respectively arranged with sealing plug, run through on the described sealing plug on being in and be provided with liquid-inlet pipe and drain pipe, the lower ending opening of described drain pipe stretches in the nutrient solution of described settling culture tube, the drain pipe of described settling culture tube is connected by linking conduit with the liquid-inlet pipe of adjacent described settling culture tube, one of them described linking conduit is provided with and is used to drive the small-sized recycle pump that described nutrient solution circulates, and is provided with the multiparameter electrode that is used to measure the settling physical and chemical parameter at least one described settling culture tube.
2. a kind of experiment incubator of measuring the settling physical and chemical parameter according to claim 1, it is characterized in that: also be provided with whipping appts in the described casing, described whipping appts comprises micromachine, active magnetic stirring rotator and driven magnetic stirring rotator, described active magnetic stirring rotator is positioned at the intravital region intermediate of described case, described settling culture tube is evenly distributed in the described casing around described active magnetic stirring rotator is rounded, and described driven magnetic stirring rotator is positioned at described settling culture tube.
3. a kind of experiment incubator of measuring the settling physical and chemical parameter according to claim 2, it is characterized in that: the stirring velocity of described active magnetic stirring rotator and described driven magnetic stirring rotator is 30r/min-180r/min.
4. a kind of experiment incubator of measuring the settling physical and chemical parameter according to claim 2 is characterized in that: described active magnetic stirring rotator and described driven magnetic stirring rotator adopt ndfeb magnetic material to manufacture.
5. a kind of experiment incubator of measuring the settling physical and chemical parameter according to claim 1, it is characterized in that: also be provided with the constent temperature heater that is used to heat the water that fills in the casing in the described casing, described constent temperature heater is connected with the outer radiator valve that is provided with of described casing.
6. a kind of experiment incubator of measuring the settling physical and chemical parameter according to claim 1, it is characterized in that: the lower ending opening of described liquid-inlet pipe stretches in the nutrient solution of described settling culture tube, and the degree of depth that described liquid-inlet pipe vertically stretches into nutrient solution vertically stretches into the degree of depth of nutrient solution greater than described drain pipe.
7. a kind of experiment incubator of measuring the settling physical and chemical parameter according to claim 1, it is characterized in that: described settling physical and chemical parameter comprises pH, Eh, DO or NH 3-N.
8. according to each described a kind of experiment incubator of measuring the settling physical and chemical parameter in the claim 1 to 7, it is characterized in that: described casing adopts transparent organic glass to manufacture, and described settling culture tube adopts transparent organic glass or polycarbonate material to manufacture.
CN 201010522057 2010-10-28 2010-10-28 Experiment incubator capable of measuring depositional physicochemical parameters Expired - Fee Related CN101984039B (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103439143A (en) * 2013-08-07 2013-12-11 中国水产科学研究院南海水产研究所 Experimental apparatus and method for researching nutrient salt exchange flux of sediment layers
CN108362833A (en) * 2018-02-05 2018-08-03 中国水产科学研究院黄海水产研究所 A method of measuring transfer of the atmospheric sedimentation heavy metal in deposit
CN109022269A (en) * 2018-10-16 2018-12-18 广东石油化工学院 Denitrification culture apparatus and its cultural method in multichannel room
CN109387390A (en) * 2018-11-23 2019-02-26 江苏大学 A kind of deposit anaerobically incubating device
CN114609048A (en) * 2022-05-12 2022-06-10 浙江大学 Sediment-water interface dissolved oxygen and pH automatic measuring device

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1025871C (en) * 1986-09-19 1994-09-07 株式会社岛津制作所 Culture device of adding pressure type
CN101580798A (en) * 2009-05-31 2009-11-18 常州三高生物技术工程设备有限公司 Photosynthetic multifunctional fermentation tank
CN101831379A (en) * 2010-05-13 2010-09-15 山东东阿阿胶股份有限公司 High-efficiency mixing renaturation device beneficial to rPA (radar Performance Analyzer) large-scale renaturation
CN201834911U (en) * 2010-10-28 2011-05-18 宁波大学 Experiment incubator capable of measuring deposit physical and chemical parameters

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1025871C (en) * 1986-09-19 1994-09-07 株式会社岛津制作所 Culture device of adding pressure type
CN101580798A (en) * 2009-05-31 2009-11-18 常州三高生物技术工程设备有限公司 Photosynthetic multifunctional fermentation tank
CN101831379A (en) * 2010-05-13 2010-09-15 山东东阿阿胶股份有限公司 High-efficiency mixing renaturation device beneficial to rPA (radar Performance Analyzer) large-scale renaturation
CN201834911U (en) * 2010-10-28 2011-05-18 宁波大学 Experiment incubator capable of measuring deposit physical and chemical parameters

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103439143A (en) * 2013-08-07 2013-12-11 中国水产科学研究院南海水产研究所 Experimental apparatus and method for researching nutrient salt exchange flux of sediment layers
CN108362833A (en) * 2018-02-05 2018-08-03 中国水产科学研究院黄海水产研究所 A method of measuring transfer of the atmospheric sedimentation heavy metal in deposit
CN108362833B (en) * 2018-02-05 2021-03-23 中国水产科学研究院黄海水产研究所 Method for determining transfer of heavy metal settled in atmospheric air in sediment
CN109022269A (en) * 2018-10-16 2018-12-18 广东石油化工学院 Denitrification culture apparatus and its cultural method in multichannel room
CN109022269B (en) * 2018-10-16 2023-08-04 广东石油化工学院 Multichannel indoor denitrification culture device and culture method thereof
CN109387390A (en) * 2018-11-23 2019-02-26 江苏大学 A kind of deposit anaerobically incubating device
CN114609048A (en) * 2022-05-12 2022-06-10 浙江大学 Sediment-water interface dissolved oxygen and pH automatic measuring device
CN114609048B (en) * 2022-05-12 2022-09-02 浙江大学 Sediment-water interface dissolved oxygen and pH automatic determination device

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