CN109387390A - A kind of deposit anaerobically incubating device - Google Patents
A kind of deposit anaerobically incubating device Download PDFInfo
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- CN109387390A CN109387390A CN201811407439.0A CN201811407439A CN109387390A CN 109387390 A CN109387390 A CN 109387390A CN 201811407439 A CN201811407439 A CN 201811407439A CN 109387390 A CN109387390 A CN 109387390A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a kind of deposit anaerobically incubating devices, are related to deposit collecting test field, including sample room, sample chamber cap, pressurizing cover plate and pressurization to silk;The sample room material is light-proof material;The sample room is hollow structure;The top and bottom of sample room are sealed by sample chamber cap;The sample room, which covers, is provided with increase cover board;The pressurizing cover plate of top and bottom connects silk by pressurization and compresses.The present invention can minimize sampling with experimentation to the human interference of sample, and the variation of native oxide reduction potential caused by penetrating into oxygen minimizes, and ensure that anaerobe in measured sample, the factors such as element of oxidizable form are consistent with primal environment height;Furthermore incubator cooperation special sampler can satisfy the sample requirement of different depth in deposit.The present invention not only has the excellent characteristics such as portability, Ke Xunhuanliyong property, but also ensures that artificial disturbance minimizes.
Description
Technical field
The present invention relates to deposit collecting test fields, are related specifically to a kind of deposit anaerobically incubating device.
Background technique
Wetlands ecosystems are the key that global substance circulation and energy flow ecotope, at present to wetlands ecosystems
Research with protection more and more attention has been paid to.Deposit (Sediments) is support wetlands ecosystems matrix, the spy with anaerobism
Property, and distinguished different from the maximum of the terrestrial soil ecosystem.However the research of deposit in wetlands ecosystems is deposited always
In a problem, i.e., in traditional earth pillar sampling research process, artificial disturbance change the original physical chemistry of deposit and
Biological nature, such as illumination cause the photodissociation of substance or the infiltration of photoresponse, oxygen to cause the oxidation of element and anaerobism micro-
The death of biology, and then cause the data finally measured and reality difference huge.
Existing sediment sampler can not perhaps accomplish anaerobism, be protected from light or can not will sink under the conditions of anaerobism, being protected from light
Product object layering, obtains the sediment sample of different depth.Such as traditional screwed sampler or geotome, it can not prevent sky
Oxygen is largely permeated into deposit in gas.And the sample devices that can accomplish opposite anaerobism, be protected from light some at present, it but can not be real
Now to the layering of column shaped deposit.During being layered after sampling, deposit causes again by a large amount of ingresss of air
Physico and biological property is deposited thoroughly to change.
In view of the above problems, the present invention provides a kind of deposit anaerobically incubating device, meet deposit maximize anaerobism,
It is protected from light and is layered the requirement of acquisition different depth deposit.
Summary of the invention
Deposit can not be layered for during Wetland Sediments traditional sampling to measure different depth sample, while again
Ensure abundant anaerobism, be protected from light the problem of data for measuring closer primal environment situation, the present invention provides a kind of deposits
Anaerobically incubating device meets the requirement that deposit maximizes anaerobism, is protected from light and is layered acquisition different depth deposit.
The present invention is achieved through the following technical solutions:
A kind of deposit anaerobically incubating device, including sample room, sample chamber cap, pressurizing cover plate and pressurization are to silk;The sample
Room material is light-proof material;The sample room is hollow structure, and the top and bottom of sample room are sealed by sample chamber cap;Institute
It states sample room and covers and be provided with pressurizing cover plate;The pressurizing cover plate of top and bottom connects silk by pressurization and compresses;The sample
The input channel and output channel being connected to sample room are provided on chamber cap.
Further, filter membrane is provided in the sample chamber cap.
Further, the sample chamber cap medial center offers centre bore, the centre bore and output channel and input
Channel communicates.
Further, setting is fluted on the inside of the sample chamber cap, and the groove is radial structure;The one of the groove
End is connected to centre bore.
Further, output channel and input channel are provided on the sample room;The centre bore that the sample room covers
It is communicated with output channel and input channel.
Further, the sample room and sample chamber cap are lighttight organic glass or 304 stainless steels.
Further, sealing ring is provided on the inside of the sample chamber cap.
Further, the sample room setting acquires deposit in sampler.
Further, the sample room is cylindrical hollow configuration.
Further, the sample room includes that multiple and different or mutually level sample room stacks setting.
1. the present apparatus according to the anaerobism of Wetland Sediments, be protected from light characteristic, will by designing opaque and sealing sample room
Sample maximumlly keeps the reduction of its native oxide and dark surrounds;By setting input channel with output channel by Bu Tong leading to
Amount and the leaching liquor containing different solvents, using incubator both ends input channel and output channel extraction deposit pore water and not
With the element of form, to measure element morphology content and modality conversion rate;Simultaneously can according to different demands in incubator mould
Microorganism needed for quasi- in-situ deposition object hatching.
2. sampling can minimize the human interference of sample with experimentation when special sampler being combined to use, and will
The variation of native oxide reduction potential caused by oxygen penetrates into minimizes, and ensure that anaerobe in measured sample, easy oxygen
It is consistent with primal environment height to change the factors such as the element of form;Furthermore incubator cooperation special sampler can satisfy in deposit
The sample requirement of different depth.
3. the present invention not only has the excellent characteristics such as portability, Ke Xunhuanliyong property, but also ensure that artificial disturbance most
A possibility that experiment of deposit in-site detecting operates indoors and new method, new approaches are provided while smallization.
4. the incubator configured in special sampler is by the opaque lucite cylinder of similar and different height, (experiment is related to
Metal assay Shi Keyong) or 304 stainless steel copper (experiment is not related to metal assay Shi Keyong) composition, as sample room.It is different or
It is mutually level that the purpose that can reach layering is set;With opaque organic glass (experiment is related to metal assay Shi Keyong) or 304
Stainless steel (experiment is not related to metal assay Shi Keyong) is that material can achieve the purpose being protected from light as sample room.
5. sample chamber cap has sealing ring, with sample room sealing cooperation to completely cut off air;Fast Segmentation various kinds in experimentation
Product room simultaneously seals (covering included filter membrane) with sample chamber cap immediately, while noticing that the channel collar extension that outputs and inputs of sample chamber cap uses
Plug is blocked.Contain radial groove on the inside of sample chamber cap.
6. the channel containing diameter 1mm inside sample chamber cap leads to sample chamber cap centre bore.This designs radial groove
Effect is to promote the solution of input to be dispersed in filter membrane surface, with guarantee deposit and solvent comprehensive engagement with react.It can
Meet and extracts Sediment Pore Water under anaerobic condition or extract the element of different shape using different solvents and measure element also
Former rate.
Detailed description of the invention
Fig. 1 be the present invention relates to a kind of deposit anaerobically incubating device structural schematic diagram;
Fig. 2 is sample chamber cap structural schematic diagram;
The cross-sectional view in the sample chamber cap only center of circle that Fig. 3 is Fig. 2;
Fig. 4 is that the structural schematic diagram in collector is applied in sample room.
Appended drawing reference is as follows:
1- pressurizing cover plate;2- sample chamber cap;The first filter membrane of 3-;The sample room 4-;5- sediment sample;The second filter membrane of 6-;7-
Input channel;8- is pressurized to silk;9- output channel;10- sealing ring;11- groove;12- sample collector.
Specific embodiment
To be understood the present invention is further, it is described further now in conjunction with attached drawing:
In conjunction with attached drawing 1, a kind of deposit anaerobically incubating device, including sample room 4, sample chamber cap 2, pressurizing cover plate 1 and pressurization
To silk 8;4 material of sample room is light-proof material;The sample room 4 is hollow structure, and the top and bottom of sample room 4 are logical
Cross the sealing of sample chamber cap 2;Pressurizing cover plate 1 is provided on the sample chamber cap 2;The pressurizing cover plate 1 of top and bottom passes through pressurization
Silk 8 is connected and is compressed;The input channel 7 and output channel 9 being connected to sample room 4 are provided on the sample chamber cap 2.
Filter membrane 3,6 is provided in the sample chamber cap 2.2 medial center of sample chamber cap offers centre bore, it is described in
Heart hole is communicated with output channel 9 and input channel 7.Fluted 11 are arranged on the inside of the sample chamber cap 2, the groove 11 is radiation
Shape structure;One end of the groove 11 is connected to centre bore.Output channel 9 and input channel 7 are provided on the sample room 4;
Centre bore on the sample chamber cap 2 is communicated with output channel 9 and input channel 7.The sample room 4 is not with sample chamber cap 2
The organic glass of light transmission or 304 stainless steels are provided with sealing ring 10 on the inside of the sample chamber cap 2.The sample room 4 is arranged
Deposit is acquired in sampler.The sample room 4 is cylindrical hollow configuration.The sample room 4 includes multiple and different or phase
Level sample room stacks setting.The anaerobically incubating device is economical and practical, recyclable, and anaerobism is micro- in it ensure that sample
The factors such as biological, oxidizable element ensure that while consistent with primal environment height the minimum of artificial disturbance, provide it is heavy
A possibility that product object in-site detecting experiment operates indoors.
In conjunction with attached drawing 1, incubator is mainly by pressurizing cover plate 1, sample chamber cap 2, the first filter membrane 3, sample room 4, deposit sample
Product 5, the second filter membrane 6, input channel 7, pressurization form silk 8 and output channel 9;Wherein, pressurizing cover plate 1 is to silk 8 with pressurization
304 stainless steel materials.Sample room 4 and sample chamber cap 2 are that the experiment of lighttight organic glass is related to metal assay Shi Keyong or 304
Stainless steel experiment is not related to metal assay Shi Keyong material.The outer diameter of sample room 4 is 50mm (can be adjusted according to requirement of experiment), interior
Diameter is that 47mm can be adjusted according to requirement of experiment, can highly be adjusted according to requirement of experiment for 20mm.
In conjunction with attached drawing 2 and 3, circular diameter is that 50mm is adjusted according to sample room in sample chamber cap 2, and outer diameter is 55mm according to sample
Room and material adjustment.1 diameter of pressurizing cover plate is 70mm according to sample room length adjustment, can be according to requirement of experiment tune with a thickness of 2mm
It is whole.Pressurization is 45mm according to sample room length adjustment, diameter 5mm to 8 length of silk.Sample chamber cap 2 is by sealing ring in incubator
10 isolation air.Radial groove 11 is contained in the inside of sample chamber cap 2 in incubator, and depth is 1mm such as Fig. 3, and sample chamber cap is not
Center of circle sectional view is crossed, quantity is more than or equal to 12.The medial center of sample chamber cap 2 has deep 2mm, diameter 2mm in the incubator
Invagination centre bore.The channel of diameter 1mm is contained in the inside of sample chamber cap 2 in the incubator, leads to sample chamber cap centre bore.
The effect of the radial groove 11 is that the solution for promoting input is dispersed in filter membrane surface, to guarantee deposit and solvent
Comprehensive engagement with react.The centre bore at 2 center of sample chamber cap is used to connect sample chamber cap inboard groove 11 and sample chamber cap 2
Inner passage.2 channel collar extension of sample chamber cap tool plug, prevents large quantity of air from entering for sealing.Incubator should be with special sampler
It is used cooperatively, achieving the effect that maximize anaerobism, being protected from light and keeping reset condition.
In conjunction with attached drawing 4, use of the sample room 4 in sample collector 12, wherein 4 size dimension of sample room be can change,
It was covered at once with sample chamber cap 2 after acquiring deposit in sample collector 12, then with pressurizing cover plate 1 and pressurization to silk 8
It is fixed, prevent the irradiation of air and sunlight.
By the opaque lucite cylinder of similar and different height, (experiment is related to gold to the incubator configured in special sampler
Belong to measurement Shi Keyong) or (experiment of 304 stainless steel copper is not related to metal assay Shi Keyong) composition, as sample room.Different or phase
It is level that the purpose that can reach layering is set;Not with opaque organic glass (experiment is related to metal assay Shi Keyong) or 304
Rust steel (experiment is not related to metal assay Shi Keyong) is that material can achieve the purpose being protected from light as sample room.Sample chamber cap tool
Sealing ring, with sample room sealing cooperation to completely cut off air;In experimentation Fast Segmentation each sample room and immediately use sample chamber cap
Sealing cover (included filter membrane), while noticing that the channel collar extension that outputs and inputs of sample chamber cap is blocked using plug.It can meet
Sediment Pore Water is extracted under anaerobic condition or the element and measurement element reduction speed of different shape are extracted using different solvents
Rate.
The embodiment is a preferred embodiment of the present invention, but present invention is not limited to the embodiments described above, not
In the case where substantive content of the invention, any conspicuous improvement that those skilled in the art can make, replacement
Or modification all belongs to the scope of protection of the present invention.
Claims (10)
1. a kind of deposit anaerobically incubating device, which is characterized in that including sample room (4), sample chamber cap (2), pressurizing cover plate (1) and
Pressurization is to silk (8);Sample room (4) material is light-proof material;The sample room (4) is hollow structure, sample room (4)
Top and bottom are sealed by sample chamber cap (2);Pressurizing cover plate (1) is provided on the sample chamber cap (2);Top and bottom
Pressurizing cover plate (1) connects silk (8) by pressurization and compresses;Be provided on the sample chamber cap (2) be connected to sample room (4) it is defeated
Enter channel (7) and output channel (9).
2. deposit anaerobically incubating device according to claim 1, which is characterized in that be provided in the sample chamber cap (2)
Filter membrane (3,6).
3. deposit anaerobically incubating device according to claim 2, which is characterized in that sample chamber cap (2) medial center
Centre bore is offered, the centre bore is communicated with output channel (9) and input channel (7).
4. deposit anaerobically incubating device according to claim 3, which is characterized in that setting on the inside of the sample chamber cap (2)
Fluted (11), the groove (11) are radial structure;One end of the groove (11) is connected to centre bore.
5. deposit anaerobically incubating device according to claim 4, which is characterized in that be provided on the sample room (4) defeated
Channel (9) and input channel (7) out;Centre bore and output channel (9) and input channel (7) phase on the sample chamber cap (2)
It is logical.
6. deposit anaerobically incubating device according to claim 1, which is characterized in that the sample room (4) and sample chamber cap
It (2) is lighttight organic glass or 304 stainless steels.
7. deposit anaerobically incubating device according to claim 1, which is characterized in that setting on the inside of the sample chamber cap (2)
There are sealing ring (10).
8. according to claim 1 to 2,4 to 7 described in any item deposit anaerobically incubating devices, which is characterized in that the sample room
(4) setting acquires deposit in sampler.
9. deposit anaerobically incubating device according to claim 1, which is characterized in that the sample room (4) is in cylinder
Hollow structure.
10. deposit anaerobically incubating device according to claim 8, which is characterized in that the sample room (4) include it is multiple not
Same or mutually level sample room stacks setting.
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CN201811407439.0A CN109387390B (en) | 2018-11-23 | 2018-11-23 | Sediment anaerobic incubator |
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