CN105467096B - A kind of biomimetic prosthetic mussel method of seawater organic contamination analyte detection - Google Patents
A kind of biomimetic prosthetic mussel method of seawater organic contamination analyte detection Download PDFInfo
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- CN105467096B CN105467096B CN201510817865.1A CN201510817865A CN105467096B CN 105467096 B CN105467096 B CN 105467096B CN 201510817865 A CN201510817865 A CN 201510817865A CN 105467096 B CN105467096 B CN 105467096B
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- 238000000034 method Methods 0.000 title claims abstract description 36
- 241000237536 Mytilus edulis Species 0.000 title claims abstract description 35
- 235000020638 mussel Nutrition 0.000 title claims abstract description 33
- 238000001514 detection method Methods 0.000 title claims abstract description 27
- 230000003592 biomimetic effect Effects 0.000 title claims abstract description 16
- 239000013535 sea water Substances 0.000 title claims abstract description 15
- 239000012491 analyte Substances 0.000 title claims abstract description 14
- 238000011109 contamination Methods 0.000 title claims abstract description 14
- -1 polyethylene Polymers 0.000 claims abstract description 30
- 239000004698 Polyethylene Substances 0.000 claims abstract description 29
- 229920000573 polyethylene Polymers 0.000 claims abstract description 29
- 229910001220 stainless steel Inorganic materials 0.000 claims abstract description 16
- 238000001914 filtration Methods 0.000 claims abstract description 11
- 238000000465 moulding Methods 0.000 claims abstract description 4
- 239000012528 membrane Substances 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000005070 sampling Methods 0.000 abstract description 6
- 230000015556 catabolic process Effects 0.000 abstract description 3
- 238000006731 degradation reaction Methods 0.000 abstract description 3
- 239000003209 petroleum derivative Substances 0.000 description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 229920001684 low density polyethylene Polymers 0.000 description 6
- 239000004702 low-density polyethylene Substances 0.000 description 6
- 239000010935 stainless steel Substances 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 5
- YMWUJEATGCHHMB-UHFFFAOYSA-N dichloromethane Substances ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 229910052640 jadeite Inorganic materials 0.000 description 4
- 150000002632 lipids Chemical class 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- 239000004215 Carbon black (E152) Substances 0.000 description 3
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical class [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 3
- 230000009931 harmful effect Effects 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N hexane Substances CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- 229930195733 hydrocarbon Natural products 0.000 description 3
- 150000002430 hydrocarbons Chemical class 0.000 description 3
- 231100000719 pollutant Toxicity 0.000 description 3
- 102000005720 Glutathione transferase Human genes 0.000 description 2
- 108010070675 Glutathione transferase Proteins 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N diphenyl Chemical compound C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 238000012851 eutrophication Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 229910052938 sodium sulfate Inorganic materials 0.000 description 2
- 235000011152 sodium sulphate Nutrition 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 239000003403 water pollutant Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 235000010290 biphenyl Nutrition 0.000 description 1
- 239000004305 biphenyl Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000002027 dichloromethane extract Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000003993 organochlorine pesticide Substances 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 description 1
- 229920002620 polyvinyl fluoride Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 210000004872 soft tissue Anatomy 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/1826—Organic contamination in water
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to a kind of biomimetic prosthetic mussel method of seawater organic contamination analyte detection, the described method comprises the following steps：Step S1, prepares four polyethylene film pipes；, be added to trioleate in the polyethylene film pipe of step S1 with liquid-transfering gun by step S2；Step S3, is sealed the both ends open of the polyethylene film pipe of step S2 with weldering molding machine；Step S4, obtains step S3 polyethylene film pipe and is fixed in stainless steel wire filtering net cylinder；Step S5, is sealed the stainless steel wire filtering net cylinder of step S4 with gauze.Its advantage shows：Hanging sampling is convenient, is not limited by natural endowment, effectively reduces the biological influence silted up.Simultaneously, the difference that natural mussel can be overcome to cause by sex, growth cycle, robustness and sea area condition, also biochemical degradation in organism can be overcome to act on, avoid certain marine site because seriously polluted mussel cannot survive the drawbacks of, the data of measure have comparativity higher, application prospect is good, can effectively carry out ocean organic contamination analyte detection.
Description
Technical field
It is that a kind of biomimetic prosthetic of seawater organic contamination analyte detection is made a gift of specifically the present invention relates to anti-raw technical field
Shellfish method.
Background technology
Littoral sea pollution about 80% in the world is petroleum hydrocarbon, polycyclic aromatic hydrocarbons, the organochlorine in Organic Pollution
Biphenyl, organic matter and eutrophication etc., wherein again especially prominent with petroleum hydrocarbon contaminated and eutrophication problem.
The concentration of organic pollutant in seawater is very low, directly determines extremely difficult.Traditional chemistry in detecting needs flower
Take substantial amounts of time and financial resources to gather, process the water sample of large volume, material to be analyzed is easily lost during analysis.In addition,
Acquired results can not reflect the harm and journey for causing and its to ecological environment the biological effectiveness part of water pollutant
Degree, can not reflect combined influence of the multiple pollutant to marine organisms.
Mussel is offshore zoobenthos widely distributed in the world, is also important marine economy species.Dwell at their battalion bottoms
Set is lived, and all has at a relatively high accumulating capability to organo-chlorine pesticide, heavy metal and radioactive element, is nearshore marine environment
The good instruction for polluting biological detection is biological.Water body and deposit can be indicated by the content for determining their internal pollution materials
Contaminated situation.But respective regions are seriously polluted, mussel can not survive, it is impossible to carry out biological sampling.In addition, mussel detection master
To be carried out according to its internal lipid enrichment organic pollution, because sex, growth period stalwartness situation are different, internal lipid content
It is quite different, so detection data comparativity is poor.
Chinese patent literature CN201510297486.4, at 03 on the applying date 2015 year 06 month, patent name is：It is a kind of quick
Detection oily waste water treatment system petrochina hydrocarbon and thalline content method, disclose a kind of quick detection oily waste water treatment system
The new method of petrochina hydrocarbon and thalline content.Under the conditions of different wave length, influence of the petroleum hydrocarbon with bacterium to light absorbs is different.Cause
Under the conditions of a specific wavelength, the total absorption value of the suspension containing petroleum hydrocarbon and bacterium is the absorption value of both petroleum hydrocarbon and bacterium for this
Sum.The method combination microorganism and petroleum hydrocarbon to having different absorption characteristics (especially its absorption maximum at different wavelengths
Peak has significant difference).It is simple easy compared with for conventional method with simple linear equation in two unknowns as detection mode in the present invention
OK, it is and relatively accurate, for the data for providing petroleum hydrocarbon and thalline content provide a kind of succinct mode.
Chinese patent literature CN201510470382.9, at 24 on the applying date 2015 year 08 month, patent name is：One kind application
The method that mussel detects water pollutant, the method for disclosing pollutant in a kind of application mussel detection breeding water body, that is, use
The changes of contents of glutathione S-transferase (GSTs) is come the method that detects pollutant in breeding water body in mussel body.It is of the invention
Method compares current instrument chemical method, and its detection limit is lower, and method is sensitiveer.And validation laboratory unanimously thinks the party
Method is accurate, sensitive, simple to operate, reproducible；Do not use poisonous organic solvent and standard items, to operating personnel safety more
It is secure, while reducing the pollution to environment；Detection time is shortened, testing cost is reduced, experiment is effectively improved
The detectability of room.By the further investigation of the project, food security biological detection system in line with international standards can be set up.
In sum, need a kind of biomimetic prosthetic mussel method of seawater organic contamination analyte detection badly, solve because of marine pollution
The problem that animal mussel high cannot be detected, and yet there are no report on this method.
The content of the invention
The purpose of the present invention is directed to deficiency of the prior art, there is provided a kind of bionical people of seawater organic contamination analyte detection
Work mussel method, solves the problems, such as to be detected because of marine pollution animal mussel high.
To achieve the above object, the present invention is adopted the technical scheme that：
A kind of biomimetic prosthetic mussel method of seawater organic contamination analyte detection, the described method comprises the following steps：
Step S1, prepares four polyethylene film pipes；
, be added to trioleate in the polyethylene film pipe of step S1 with liquid-transfering gun by step S2；
Step S3, is sealed the both ends open of the polyethylene film pipe of step S2 with weldering molding machine；
Step S4, obtains step S3 polyethylene film pipe and is fixed in stainless steel wire filtering net cylinder；
Step S5, is sealed the stainless steel wire filtering net cylinder of step S4 with gauze.
Polyethylene film pipe range 22cm, 5cm wide, 50 μm of thickness in the step S1.
, it is necessary to be laid on desktop after trioleate in the step S2 is added in polyethylene film pipe, by vessel
Accumbency takes, and extruding makes trioleate be uniformly distributed in the whole polyethylene film pipe except mouth of pipe two ends 1cm.
It is 600 μ l that each polyethylene film pipe in the step S2 need to add the volume of trioleate.
Seal length in the step S3 is each 1cm in two ends.
Stainless steel filter screen cylinder in the step S4 is 5 mesh, the built-in upper and lower cross of fixation, cross distance webs cylinder end
Mouth has the distance of 1cm, for fixing 4 polyethylene film pipes simultaneously.
Gauze in the step S5 is 300 mesh, it is necessary to stainless steel filter screen cylinder is fully sealed, and is individual layer sealing.
The invention has the advantages that：
1st, the biomimetic prosthetic mussel method of a kind of seawater organic contamination analyte detection of the invention, makes simple, hanging sampling
It is convenient, do not limited by natural endowment；2nd, natural mussel can be overcome to cause because of sex, growth cycle, robustness and sea area condition
Difference, can also overcome in organism biochemical degradation to act on, the data of measure have comparativity higher；
3rd, avoid certain marine site because seriously polluted mussel cannot survive the drawbacks of, be equally applicable to sudden pollusion atmospheric
The detection of event；
4th, the low-density polyethylene film pipe in sampling apparatus is fixed and is placed in and is surrounded by the stainless steel filter screen of gauze cylinder
Portion, reduces the biological harmful effect for silting effect up caused by flow rate of water flow and Suspended Matter in Water.
Brief description of the drawings
Accompanying drawing 1 is a kind of biomimetic prosthetic mussel method flow schematic diagram of seawater organic contamination analyte detection of the invention.
Specific embodiment
The specific embodiment that the present invention is provided is elaborated below in conjunction with the accompanying drawings.
Embodiment 1
Fig. 1 is refer to, Fig. 1 is that a kind of biomimetic prosthetic mussel method flow of seawater organic contamination analyte detection of the invention shows
It is intended to.A kind of biomimetic prosthetic mussel method of seawater organic contamination analyte detection, the described method comprises the following steps：
Step S1, prepares four middle low-density polyethylene film pipes, and according to test of many times, preferably middle low density polyethylene (LDPE) is thin
Membrane tube 22cm long, 5cm wide, 50 μm of thickness, its size thickness and membrane area, the speed for being enriched with organic matter are high.
, be added to trioleate in the middle low-density polyethylene film pipe of step S1 with liquid-transfering gun by step S2；Wherein, three oil
, it is necessary to be laid on desktop after acid esters is added in polyethylene film pipe, taken by vessel accumbency, extruding makes trioleate uniform
It is distributed in the whole polyethylene film pipe except mouth of pipe two ends 1cm.Trioleate is HMW, to petroleum hydrocarbon collection and in advance
In processing procedure, it is difficult to cause chelating media divergence loss；Trioleate is the fat of low melting point, in hot climate waters not
Easily play, solidification is difficult in freezing weather waters, there is basin pollution detection and research；According to test of many times, preferably in each
Low-density polyethylene film pipe need to add the volume of trioleate for 600 μ l, under its volume in 22cm long, 5cm wide, thickness
Absorption rate in 50 μm of polyethylene film pipes is high.
Step S3, is sealed the both ends open of the polyethylene film pipe of step S2 with weldering molding machine, and seal length is that two ends are each
1cm；
Step S4, obtains step S3 polyethylene film pipe and is fixed in stainless steel wire filtering net cylinder, stainless steel filter screen cylinder
It is 5 mesh, there is the distance of 1cm the built-in upper and lower cross of fixation, cross distance webs cylinder port, for fixing low close in 4 simultaneously
Degree polyethylene film pipe, by stainless steel wire filtering net cylinder and cross, can eliminate attachment, and the influence that biological pollution is caused has
Effect ground suppresses the interference of biological pollution.
Step S5, is sealed the stainless steel wire filtering net cylinder of step S4 with gauze, and gauze is 300 mesh, it is necessary to by stainless steel
Filtering net cylinder is fully sealed, and is individual layer sealing, reduces the biology effect of silting up caused by flow rate of water flow and Suspended Matter in Water
Harmful effect.
Comparative example 1
Natural jadeite mussel is sampled, and opens shell, is divided into four groups and is tested, each group of parameter all same, and each group is pressed
Carried out according to following procedures, soft tissue 8g (after precise to 0.001g) is put into special 40cm3Special polyvinyl fluoride
In pipe, 20g anhydrous sodium sulfates are added, add 11.68 μ g/cm3, C20Internal standard 1cm3.With bar-shaped crushing machine head soft group of mussel
Knit, sodium sulphate, internal standard compound C20Stir, add 28cm3Dichloromethane, high-speed stirred, on centrifuge (1000r/min)
Centrifugation 5 minutes, topples over upper strata dichloromethane extract, is repeated twice extraction, is not developed the color with dichloromethane solution only, 3 dichloromethanes
Alkane extract is combined, and carries out convolution and is concentrated under reduced pressure into 5cm3, add 30cm3N-hexane, continues to be concentrated into 1-2cm3, then
By diatomite chromatography, collect n-hexane leacheate and use and make petroleum hydrocarbon analysis.
Result is compared
The seawater sample petroleum hydrocarbon testing result of table 1
Note：Bracket data are esters petroleum hydrocarbon concentration in the every gram of mussel body for calculating.
From four groups of experiments of table 1, compare in biomimetic prosthetic mussel body in the petroleum hydrocarbon concentration and natural jadeite mussel of lipid
Trioleate includes petroleum hydrocarbon concentration it is known that it is 2.95 [× 10 to contain petroleum hydrocarbon concentration in biomimetic prosthetic mussel body-3(m/
M)], 2.87 [× 10-3(m/m)]、3.31[×10-3(m/m)]、3.01[×10-3(m/m)], its petroleum hydrocarbon concentration average out to
3.04[×10-3(m/m) it is 0.63 [× 10], to contain petroleum hydrocarbon concentration in natural jadeite mussel-3(m/m)]、0.48[×10-3
(m/m)]、0.9[×10-3(m/m)]、0.46[×10-3], (m/m) its petroleum hydrocarbon concentration average out to 0.62 [× 10-3(m/m)],
By comparing, in biomimetic prosthetic mussel body the petroleum hydrocarbon concentration of lipid be natural jadeite mussel in trioleate include oil
4.9 times of hydrocarbon concentration, learn that biomimetic prosthetic mussel has stronger adsorption and enrichment ability.
A kind of biomimetic prosthetic mussel method of seawater organic contamination analyte detection of the invention, makes simple, hangs sampling side
Just, do not limited by natural endowment；The difference that natural mussel can be overcome to cause by sex, growth cycle, robustness and sea area condition
It is different, can also overcome biochemical degradation in organism to act on, the data of measure have comparativity higher；Certain marine site is avoided because of dirt
The drawbacks of contaminating serious mussel and cannot survive, the detection of sudden pollusion atmospheric event is equally applicable to；It is low close in sampling apparatus
Degree polyethylene film pipe fix and be placed in be surrounded by gauze stainless steel filter screen cylinder it is internal, reduce and hang in flow rate of water flow and water body
The biological harmful effect for silting effect up caused by float.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
Member, on the premise of the inventive method is not departed from, can also make some improvement and supplement, and these are improved and supplement also should be regarded as
Protection scope of the present invention.
Claims (5)
1. a kind of biomimetic prosthetic mussel method of seawater organic contamination analyte detection, it is characterised in that methods described includes following step
Suddenly：
Step S1, prepares four polyethylene film pipes；
, be added to trioleate in the polyethylene film pipe of step S1 with liquid-transfering gun by step S2, and it is thin that trioleate is added to polyethylene
, it is necessary to be laid on desktop after in membrane tube, by vessel accumbency pipe, extruding makes trioleate be uniformly distributed in except mouth of pipe two ends
The whole polyethylene film pipe of 1cm；
Step S3, is sealed the both ends open of the polyethylene film pipe of step S2 with weldering molding machine；
Step S4, the polyethylene film pipe of step S3 is fixed in stainless steel wire filtering net cylinder, and stainless steel wire filtering net cylinder is 5
There is the distance of 1cm mesh, the built-in upper and lower cross of fixation, cross distance webs cylinder port, for fixing 4 polyethylene films simultaneously
Pipe；
Step S5, the stainless steel wire filtering net cylinder of step S4 is fully sealed with gauze, and is individual layer sealing.
2. method according to claim 1, it is characterised in that the polyethylene film pipe range 22cm in the step S1, it is wide
5cm, 50 μm of thickness.
3. method according to claim 1, it is characterised in that each the polyethylene film pipe in the step S2 needs to add
The volume of trioleate is 600 μ l.
4. method according to claim 1, it is characterised in that the seal length in the step S3 is each 1cm in two ends.
5. method according to claim 1, it is characterised in that the gauze in the step S5 is 300 mesh.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510817865.1A CN105467096B (en) | 2015-11-23 | 2015-11-23 | A kind of biomimetic prosthetic mussel method of seawater organic contamination analyte detection |
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| CN201510817865.1A CN105467096B (en) | 2015-11-23 | 2015-11-23 | A kind of biomimetic prosthetic mussel method of seawater organic contamination analyte detection |
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| CN1804575A (en) * | 2005-12-27 | 2006-07-19 | 南开大学 | Semipermeable membrane for sampling organic contaminant in enriched water environment |
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