CN109387390B - Sediment anaerobic incubator - Google Patents
Sediment anaerobic incubator Download PDFInfo
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- CN109387390B CN109387390B CN201811407439.0A CN201811407439A CN109387390B CN 109387390 B CN109387390 B CN 109387390B CN 201811407439 A CN201811407439 A CN 201811407439A CN 109387390 B CN109387390 B CN 109387390B
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- sample chamber
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
Abstract
The invention discloses a sediment anaerobic incubator, which relates to the field of sediment acquisition and testing and comprises a sample chamber, a sample chamber cover, a pressurizing cover plate and a pressurizing pair wire; the sample chamber is made of a light-tight material; the sample chamber is of a hollow structure; the upper end and the lower end of the sample chamber are sealed by a sample chamber cover; an additional cover plate is arranged on the sample chamber cover; the pressurizing cover plates at the upper end and the lower end are connected and compressed through pressurizing and wire aligning. The invention can minimize the artificial interference to the sample in the sampling and experiment process, and minimize the change of the original oxidation-reduction potential caused by oxygen permeation, thereby ensuring that factors such as anaerobic microorganisms and elements in an easily-oxidized form in the measured sample are highly consistent with the original environment; in addition, the incubator is matched with a special sampler, so that the sampling requirements of different depths in sediments can be met. The invention not only has the excellent characteristics of portability, recyclability and the like, but also ensures the minimization of artificial disturbance.
Description
Technical Field
The invention relates to the field of sediment collection and testing, in particular to a sediment anaerobic incubator.
Background
The wetland ecosystem is a key ecological area for global material circulation and energy flow, and the research and protection of the wetland ecosystem are more and more concerned. Sediments (segments) are the substrate for supporting wetland ecosystem, have anaerobic property and are the biggest difference from land soil ecosystem. However, the research on the sediments in the wetland ecosystem has a problem that the original physicochemical and biological characteristics of the sediments are changed by artificial disturbance in the traditional soil column sampling research process, for example, photolysis or photosensitive reaction of substances caused by illumination, oxidation of elements caused by oxygen permeation and death of anaerobic microorganisms, so that finally measured data is greatly different from the actual situation.
The existing sediment sampler can not realize anaerobic and photophobic operation or can not layer the sediment under the anaerobic and photophobic conditions to obtain sediment samples with different depths. For example, a traditional spiral sampler or an earth drill cannot prevent oxygen in the air from permeating into sediments in a large quantity. However, some sampling devices which can achieve relative anaerobism and light resistance cannot realize the layering of the columnar sediments. In the process of layering after sampling, a large amount of sediment contacts with air, so that the physicochemical and biological properties of the sediment are thoroughly changed.
Aiming at the problems, the invention provides a sediment anaerobic incubator which meets the requirements of sediment maximization anaerobic property, light shielding and layered collection of sediments at different depths.
Disclosure of Invention
The invention provides a sediment anaerobic incubator, which aims to solve the problems that sediments cannot be layered to measure samples with different depths in the traditional sampling process of wetland sediments, and simultaneously, data closer to the original environment condition is measured by sufficient anaerobic and light-proof measurement.
The invention is realized by the following technical scheme:
a sediment anaerobic incubator comprises a sample chamber, a sample chamber cover, a pressurizing cover plate and a pressurizing pair wire; the sample chamber is made of a light-tight material; the sample chamber is of a hollow structure, and the upper end and the lower end of the sample chamber are sealed by a sample chamber cover; a pressurizing cover plate is arranged on the sample chamber cover; the pressurizing cover plates at the upper end and the lower end are connected and compressed through pressurizing paired wires; and the sample chamber cover is provided with an input channel and an output channel which are communicated with the sample chamber.
Further, a filter membrane is arranged in the sample chamber cover.
Furthermore, a center hole is formed in the center of the inner side of the sample chamber cover, and the center hole is communicated with the output channel and the input channel.
Further, a groove is formed in the inner side of the sample chamber cover, and the groove is of a radial structure; one end of the groove is communicated with the central hole.
Furthermore, an output channel and an input channel are arranged on the sample chamber; the central hole on the sample chamber cover is communicated with the output channel and the input channel.
Further, the sample chamber and the sample chamber cover are made of light-tight organic glass or 304 stainless steel.
Further, a sealing ring is arranged on the inner side of the sample chamber cover.
Further, the sample chamber is arranged in the sampler for collecting sediment.
Further, the sample chamber is a cylindrical hollow structure.
Further, the sample chamber comprises a plurality of sample chamber stacks with different or same heights.
1. The device maximally maintains the original redox and dark environment of the sample by designing the light-tight and sealed sample chamber according to the anaerobic and light-tight characteristics of the wetland sediments; extracting pore water and elements with different forms in sediments by arranging an input channel and an output channel through different fluxes and leaching liquors containing different solvents and utilizing the input channel and the output channel at two ends of an incubator so as to determine the element form content and the form conversion rate; meanwhile, the microorganisms required by the in-situ sediment incubation can be simulated in the incubator according to different requirements.
2. When the device is used in combination with a special sampler, the device can minimize the artificial interference on samples in the sampling and experiment processes, minimize the change of the original oxidation-reduction potential caused by oxygen permeation, and ensure that factors such as anaerobic microorganisms and elements in an easily-oxidized form in the measured samples are highly consistent with the original environment; in addition, the incubator is matched with a special sampler, so that the sampling requirements of different depths in sediments can be met.
3. The invention not only has the excellent characteristics of portability, recyclability and the like, but also provides the possibility, new method and new idea of indoor operation of the sediment in-situ determination experiment while ensuring the minimization of artificial disturbance.
4. The hatcher arranged in the special sampler is composed of opaque organic glass cylinders (available when the experiment relates to metal measurement) or 304 stainless steel copper (available when the experiment does not relate to metal measurement) with different or same heights, namely the sample chamber. The purpose of layering can be achieved by arranging different or same heights; the sample chamber is made of light-proof organic glass (available in experiments relating to metal measurement) or 304 stainless steel (available in experiments not relating to metal measurement), so that the aim of avoiding light can be fulfilled.
5. The sample chamber cover is provided with a sealing ring which is in sealing fit with the sample chamber to isolate air; in the experimental process, each sample chamber is quickly divided and immediately sealed by a sample chamber cover (a filter membrane is attached in the cover), and meanwhile, the outer openings of the input and output channels of the sample chamber cover are sealed by plugs. The sample chamber lid contains radial grooves on the inside.
6. The sample chamber lid contains a 1mm diameter channel inside, leading to the sample chamber lid central bore. The radial grooves are designed to promote the input solution to be uniformly dispersed on the surface of the filter membrane so as to ensure that the sediment and the solvent are in full contact and react. Can meet the requirements of extracting sediment pore water under the anaerobic condition, or extracting elements with different forms by using different solvents and measuring the reduction rate of the elements.
Drawings
FIG. 1 is a schematic structural diagram of a sediment anaerobic incubator according to the present invention;
FIG. 2 is a schematic view of a sample chamber lid;
FIG. 3 is a cross-sectional view of the sample chamber lid of FIG. 2, but without the center of the circle;
FIG. 4 is a schematic structural diagram of a sample chamber applied in a collector.
The reference numbers are as follows:
1-a pressurizing cover plate; 2-sample chamber lid; 3-a first filter membrane; 4-a sample chamber; 5-sediment sample; 6-a second filter membrane; 7-an input channel; 8-pressurizing and aligning; 9-an output channel; 10-a sealing ring; 11-a groove; 12-sample collector.
Detailed Description
For a further understanding of the present invention, reference will now be made to the following descriptions taken in conjunction with the accompanying drawings:
with reference to the attached drawing 1, the sediment anaerobic incubator comprises a sample chamber 4, a sample chamber cover 2, a pressurizing cover plate 1 and a pressurizing pair wire 8; the sample chamber 4 is made of a light-tight material; the sample chamber 4 is of a hollow structure, and the upper end and the lower end of the sample chamber 4 are sealed by the sample chamber cover 2; a pressurizing cover plate 1 is arranged on the sample chamber cover 2; the pressurizing cover plates 1 at the upper end and the lower end are connected and pressed tightly through pressurizing paired wires 8; the sample chamber cover 2 is provided with an input channel 7 and an output channel 9 which are communicated with the sample chamber 4.
And filter membranes 3 and 6 are arranged in the sample chamber cover 2. The center of the inner side of the sample chamber cover 2 is provided with a center hole which is communicated with the output channel 9 and the input channel 7. A groove 11 is formed in the inner side of the sample chamber cover 2, and the groove 11 is of a radial structure; one end of the groove 11 is communicated with the central hole. An output channel 9 and an input channel 7 are arranged on the sample chamber 4; the central hole in the sample chamber cover 2 communicates with the output channel 9 and the input channel 7. The sample chamber 4 and the sample chamber cover 2 are made of light-tight organic glass or 304 stainless steel, and a sealing ring 10 is arranged on the inner side of the sample chamber cover 2. The sample chamber 4 is arranged in a sampler for collecting sediment. The sample chamber 4 is a cylindrical hollow structure. The sample chamber 4 comprises a plurality of sample chamber stacks of different or equal height. The anaerobic incubator is economical, practical and recyclable, ensures the minimization of artificial disturbance while ensuring that factors such as anaerobic microorganisms and easily-oxidized elements in a sample are highly consistent with the original environment, and provides the possibility of indoor operation of a sediment in-situ determination experiment.
With reference to the attached drawing 1, the incubator mainly comprises a pressurizing cover plate 1, a sample chamber cover 2, a first filter membrane 3, a sample chamber 4, a sediment sample 5, a second filter membrane 6, an input channel 7, a pressurizing couple wire 8 and an output channel 9; wherein, the pressurizing cover plate 1 and the pressurizing pair wire 8 are made of 304 stainless steel. The sample chamber 4 and the sample chamber cover 2 are made of opaque organic glass and can be used when metal measurement is involved in experiments or 304 stainless steel and does not relate to materials used when metal measurement is involved in experiments. The outer diameter of the sample chamber 4 is 50mm (can be adjusted according to the experiment requirement), the inner diameter is 47mm and can be adjusted according to the experiment requirement, and the height is 20mm and can be adjusted according to the experiment requirement.
With reference to fig. 2 and 3, the inner circle diameter of the sample chamber cover 2 is 50mm according to the sample chamber, and the outer diameter is 55mm according to the sample chamber and the material. The diameter of the pressurizing cover plate 1 is 70mm and can be adjusted according to the length of the sample chamber, and the thickness of the pressurizing cover plate is 2mm and can be adjusted according to the experiment requirement. The length of the pressurizing pair wire 8 is 45mm, the pressurizing pair wire is adjusted according to the length of the sample chamber, and the diameter of the pressurizing pair wire is 5 mm. The sample chamber cover 2 in the incubator is sealed from air by a seal 10. The inner side of the sample chamber cover 2 in the incubator contains radial grooves 11 with the depth of 1mm as shown in figure 3, and the number of the sample chamber covers is more than or equal to 12 in a section view not passing through the circle center. The inner side center of the sample chamber cover 2 in the incubator is provided with an invagination center hole with the depth of 2mm and the diameter of 2 mm. The interior of the sample chamber cover 2 in the incubator contains a channel with a diameter of 1mm, leading to the central hole of the sample chamber cover. The radial grooves 11 serve to promote the uniform dispersion of the incoming solution on the surface of the filter membrane to ensure the thorough contact and reaction of the deposits with the solvent. The central hole in the centre of the sample chamber cover 2 is used to connect the recess 11 inside the sample chamber cover with the internal channel of the sample chamber cover 2. The sample chamber lid 2 access port outer plug is used to seal against the ingress of large volumes of air. The incubator should be used in conjunction with a dedicated sampler to maximize anaerobic, light-tight and original state maintenance.
Referring to fig. 4, sample chamber 4 is shown in use in sample collector 12, wherein sample chamber 4 is of variable size and is covered with sample chamber cover 2 immediately after collection of sediment in sample collector 12, and is then secured with cover plate 1 and wire 8 to prevent exposure to air and sunlight.
The hatcher arranged in the special sampler is composed of opaque organic glass cylinders (available when the experiment relates to metal measurement) or (available when the experiment of 304 stainless steel and copper does not relate to metal measurement) with different or same heights, namely the sample chamber. The purpose of layering can be achieved by arranging different or same heights; the sample chamber is made of light-proof organic glass (available when the experiment relates to metal measurement) or 304 stainless steel (available when the experiment does not relate to metal measurement), so that the aim of avoiding light can be fulfilled. The sample chamber cover is provided with a sealing ring which is in sealing fit with the sample chamber to isolate air; in the experiment process, each sample chamber is quickly divided, a sample chamber cover sealing cover (an inner attached filter membrane) is immediately used, and meanwhile, the outer openings of the input channel and the output channel of the sample chamber cover are sealed by using plugs. Can meet the requirements of extracting sediment pore water under the anaerobic condition, or extracting elements with different forms by using different solvents and measuring the reduction rate of the elements.
The present invention is not limited to the above-described embodiments, and any obvious improvements, substitutions or modifications can be made by those skilled in the art without departing from the spirit of the present invention.
Claims (6)
1. A sediment anaerobic incubator is characterized by comprising a sample chamber (4), a sample chamber cover (2), a pressurizing cover plate (1) and a pressurizing pair wire (8); the material of the sample chamber (4) is light-tight material; the sample chamber (4) is of a hollow structure, and the upper end and the lower end of the sample chamber (4) are sealed by the sample chamber cover (2); a pressurizing cover plate (1) is arranged on the sample chamber cover (2); the pressurizing cover plates (1) at the upper end and the lower end are connected and pressed tightly through pressurizing paired wires (8); an input channel (7) and an output channel (9) which are communicated with the sample chamber (4) are arranged on the sample chamber cover (2); a central hole is formed in the center of the inner side of the sample chamber cover (2), and the central hole is communicated with the output channel (9) and the input channel (7); a groove (11) is formed in the inner side of the sample chamber cover (2), and the groove (11) is of a radial structure; one end of the groove (11) is communicated with the central hole.
2. Sediment anaerobic incubator according to claim 1, characterized in that the sample chamber (4) and sample chamber cover (2) are of light-tight plexiglas or 304 stainless steel.
3. Sediment anaerobic incubator according to claim 1, characterized in that inside the sample chamber cover (2) is provided a sealing ring (10).
4. Sediment anaerobic incubator according to any of claims 1-3, characterized in that the sample chamber (4) is arranged in a sampler for collecting sediment.
5. Sediment anaerobic incubator according to claim 1, characterized in that the sample chamber (4) is a cylindrical hollow structure.
6. Sediment anaerobic incubator according to claim 4, characterized in that the sample chamber (4) comprises a plurality of sample chamber stacks of different or equal height.
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| CN201811407439.0A CN109387390B (en) | 2018-11-23 | 2018-11-23 | Sediment anaerobic incubator |
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| CN201811407439.0A CN109387390B (en) | 2018-11-23 | 2018-11-23 | Sediment anaerobic incubator |
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| CN109387390B true CN109387390B (en) | 2022-04-15 |
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