CN103293233B - Method and device for measuring anaerobic ammonia oxidation rate and contribution rate of anaerobic ammonia oxidation for removing nitrogen in water/sediment system in situ - Google Patents

Abstract

The invention discloses a method and a device for measuring the anaerobic ammonia oxidation rate and the contribution rate of anaerobic ammonia oxidation for removing nitrogen in a water/sediment system in situ. The device comprises a pipe (11), a rubber plug (10) and accessories, wherein both ends of the pipe (11) are open, the rubber plug (10) is matched with the pipe (11), and the accessories are distributed on the rubber plug (10) in a penetrating mode; the rubber plug (10) is completely sealed with the pipe (11); the accessories on the rubber plug include a pressure regulating device, a stirring device and a sampling device. According to the method and the device, a 15N isotopic tracing method is utilized to in-situ culture and collect overlying water of homogeneous sediments within a certain space range of the water/sediment system, thereby further measuring soluble 29N2 and 30N2 to calculate the anaerobic ammonia oxidation rate and the contribution rate of the anaerobic ammonia oxidation for removing the nitrogen within a certain area of the water/sediment system. The method and the device can satisfy environmental conditions of simulating in-situ temperature, illumination, states of nutrient elements and the like, and typical samples are collected to be used for measuring the anaerobic ammonia oxidation rate and the contribution rate of the anaerobic ammonia oxidation for removing the nitrogen.

Description

In-site detecting water/sediment Anammox speed and the method and apparatus to nitrogen removal contribution rate thereof

Technical field

The present invention relates to a kind of in-site detecting water/sediment Anammox speed and the method and apparatus to nitrogen removal contribution rate thereof.

Background technology

Anammox is one of discovery that environmental science shocked most in nearly ten years, and correlative study achievement is delivered, because this process completely avoid N in nitrogen cycle process on periodicals such as " Nature " ₂the generation of O, is therefore subject to the extensive concern of international community.Anammox is the process (NH using nitrite as electron accepter, ammonia nitrogen direct oxidation being produced nitrogen by anaerobic ammonia oxidizing bacteria (inorganization energy autotroph) ₄ ⁺+ NO ₂ ^-→ N ₂+ 2H ₂o).N is produced with traditional denitrification ₂approach (NO ₃ ^-→ NO ₂ ^-→ NO → N ₂o → N ₂) compare, Anammox is the another important product N of nature ₂approach.

Anaerobic ammonium oxidation process is found in denitrification fluidized bed by Mulder etc. the earliest, is mainly used in sewage treatment area subsequently, in the treatment of waste water because it does not need to consume organism, oxygen, and avoids N ₂the generation of O, is a kind of biological denitrification process the most economic at present, has a wide range of applications future.Along with the in succession discovery of anaerobic ammonium oxidation process in the natural ecosystems such as marine ecosystems, terrestrial ecosystems, its vital role is more and more subject to the common concern of international community.Currently used molecular biology method can not meet anaerobic ammonium oxidation process removes contribution research to nature nitrogen, be badly in need of adopting the biogeochemistry such as tagging to measure Anammox speed, and then its vital role in nature nitrogen cycle clear and definite.Currently used Anammox rate determination method is only limitted to indoor culture experiment or gathers the half original position experiment that original position post sample carries out indoor cultivation.Carry out complete Culture in situ experiment, then gathering original position sample determination Anammox speed is current problem demanding prompt solution.

Summary of the invention

The object of this invention is to provide a kind of in-site detecting water/sediment Anammox speed and the method and apparatus to nitrogen removal contribution rate thereof.

In-site detecting Anammox speed provided by the invention and the device to nitrogen removal contribution rate thereof, comprise the pipe 11 of open at both ends, the plug 10 mated with described pipe 11 and the accessory of disposed therethrough on described plug 10;

Described plug 10 seals completely with described pipe 11;

Accessory on described plug comprises pressure adjustment assembly, stirring apparatus and sampler;

Wherein, described stirring apparatus comprises the drip-proof motors 6, transmission shaft 7 and the stirrer 9 that connect in turn; Described drip-proof motors 6 is positioned at the center of described plug 10, and through described plug 10; Described transmission shaft 7 and stirrer 9 are positioned at described pipe 11;

Described drip-proof motors 6 seals completely with the joint glass cement of described plug 10;

Described sampler comprises the sampling injector 3, T-valve 2, silicone tube 4 and the sampling glass tube 8 being positioned at described pipe 11 that connect in turn from top to bottom.

Above-mentioned in-site detecting Anammox speed and also can being only made up of said modules the device that nitrogen removes contribution rate.

Wherein, described pressure adjustment assembly is preferably the syringe 1 without piston, and the effect of this pressure adjustment assembly ensures the air pressure balance inside and outside stainless-steel tube.In use, need first by original position water fully with the described syringe 1 without piston that reinjects after high-pure helium aeration.

Described pipe 11 has higher-strength, can ensure that it inserts sediment certain depth to be measured.Described plug 10 can seal completely with pipe 11, ensures this columnar water/sediment with extraneous without mass exchange.

The object of T-valve 2 and a sampling injector 3 that the two ends of described silicone tube 4 connect a Quality control collection capacity be respectively ensure with extraneous without the condition of mass exchange under overlying water sample in quantitative collection cylinder.

Described mensuration Anammox speed and the device of contribution rate is removed to nitrogen also comprise the power supply 5 be connected with described drip-proof motors 6, to provide described drip-proof motors 6 required drive.The transmission shaft 7 be connected with drip-proof motors 6 and stirrer 9 are for stirring and evenly mixing sediment overlying water.In addition, described drip-proof motors 6 seals with the joint glass cement of described plug 10, to ensure impermeability.

Mensuration Anammox speed provided by the invention and the method to nitrogen removal contribution rate thereof, comprise the steps:

1 the pipe 11 in aforementioned means inserted water to be measured/sediment 30-50cm stable after, add in the water of described pipe 11 ¹⁵nO ₃ ^-the aqueous solution of salt, again the plug 10 in arbitrary for claim 1-4 described device and the accessory on described plug 10 are all filled in the upper end of described pipe 11, make the lower end of described sampling glass tube 8 apart from described water to be measured/3-8cm place, sediment interface, and seal the gap of described plug 10 and described pipe 11;

2 utilize described stirring apparatus stir after, oxygen-free water is injected described pressure adjustment assembly, open described T-valve 2, after extracting the air in described sampling glass tube 8 out with described sampling injector 3, close described T-valve 2, pull out described sampling injector 3, more described sampling injector 3 is inserted in described T-valve 2 after draining air, open described T-valve 2 and extract the water sample of described water/sediment to be measured out in Dewar bottle, measure in the water sample of water/sediment described in different time points ²⁹n ₂with ³⁰n ₂concentration, and then according to described ²⁹n ₂with ³⁰n ₂concentration, calculate the contribution rate that described Anammox speed and Anammox are removed nitrogen.

In step 2 described in said method, measure in the water sample of water/sediment described in different time points ²⁹n ₂with ³⁰n ₂the method of concentration comprise the steps:

Is opened the water sample that described T-valve 2 extracts described water/sediment to be measured out first time and be designated as first time point in the time point of Dewar bottle, measure in the water sample of described water/sediment during this first time point ²⁹n ₂with ³⁰n ₂concentration, be respectively ²⁹c ₁with ³⁰c ₁, then when measuring 1+n time point in the same way respectively described water/sediment water sample in ²⁹n ₂with ³⁰n ₂concentration, be respectively ²⁹c _1+nwith ³⁰c _1+n, wherein, n is the integer of 1-5, and preferred n is 4; Adjacent two time points be spaced apart 8-16 hour, preferably 12 hours.

Described in described basis ²⁹n ₂with ³⁰n ₂concentration calculate described Anammox speed and the method for Anammox to the contribution rate that nitrogen is removed comprises the steps:

In water sample according to water/sediment described during gained different time points ²⁹n ₂with ³⁰n ₂concentration, calculate in described water/sediment unit area ²⁹n ₂with ³⁰n ₂generation speed, be respectively p ²⁹n ₂ ^in-situand p ³⁰n ₂ ^in-situ, by described p ²⁹n ₂ ^in-situand p ³⁰n ₂ ^in-situsubstitution formula I and formula II, obtains ²⁸n ₂, ²⁹n ₂with ³⁰n ₂total generation speed TN ₂p, Anammox produce ²⁹n ₂with ³⁰n ₂speed Anmx, Anammox contribution rate Ra that nitrogen is removed ^in-situ:

Ra ^in-situ＝Anmx/TN ₂P

TN ₂p=2r ₁₄[p ²⁹n ₂ ^in-situ+ p ³⁰n ₂ ^in-situ(1-r ₁₄)] formula I

Anmx=2r ₁₄(p ²⁹n ₂ ^{in situ}-2r ₁₄p ³⁰n ₂ ^{in situ}) formula II

Described p ²⁹n ₂ ^in-situand p ³⁰n ₂ ^in-situunit be nmol N ₂m ^-2h ^-1; Described TN ₂the unit of P and Anmx is nmol N m ^-2h ^-1;

In described formula I and formula II, r ₁₄for in reduction film micro area ¹⁴nO ₃ ^-with ¹⁵nO ₃ ^-ratio, r ₁₄calculated by formula III and obtain:

$r_{14}=\frac{(1-\mathrm{Ra})\left(\frac{p^{29}{N}_2}{p^{30}{N}_2}\right)-\mathrm{Ra}}{(2-\mathrm{Ra})}$ Formula III

In described formula III, p ²⁹n ₂, p ³⁰n ₂, Ra by formula IV to formula VI calculate and obtain:

$D_{\mathrm{total}}=p^{30}{N}_2\×F_N^{-2}$ Formula IV

$A_{\mathrm{total}}=F_N^{-1}\×(p^{29}{N}_2+2\×(1-F_N^{-1})\×p^{30}{N}_2)$ Formula V

$\mathrm{Ra}=\frac{A_{\mathrm{total}}}{A_{\mathrm{total}}+D_{\mathrm{total}}}\×100\%$ Formula VI

Described formula IV in formula VI,

D _totalfor being produced by denitrification ²⁸n ₂, ²⁹n ₂with ³⁰n ₂speed, unit is nmol N ₂g ^-1h ^-1;

A _totalfor being produced by Anammox ²⁸n ₂with ²⁹n ₂speed, unit is nmol N ₂g ^-1h ^-1;

Ra is for produce N by Anammox ₂ratio;

P ²⁹n ₂for in indoor mud test ²⁹n ₂generation speed, unit is nmol N ₂g ^-1h ^-1;

P ³⁰n ₂for in indoor mud test ³⁰n ₂generation speed, unit is nmol N ₂g ^-1h ^-1;

F _nfor in indoor mud test nitrate used ¹⁵the abundance of N.

Described step 1) in, described in ¹⁵nO ₃ ^-the consumption of the aqueous solution of salt is for making NO in described pipe 11 overlying water ₃ ^--N concentration is 5-10mg L ^-1.

Described step 2) in, in the water sample of described water/sediment ²⁹n ₂with ³⁰n ₂the pre-treating method of concentration determination is helium Head space.Described helium Head space is specially: the 12mL Labco bottle injection of high-purity He gas (99.999%) being filled water sample, discharge 8mL water sample (remaining 4mL in sample bottle) with a syringe simultaneously, violent shake sample bottle 1min, makes the N be dissolved in water sample ₂discharge, then use in intestinal bacteria instrument (Gasbench-MAT253) working sample bottle ²⁹n ₂with ³⁰n ₂concentration.

In described whipping step, the time is 15-30min, and speed is 50-70rpm.

The solder(ing)acid that concentration is 50 grams per milliliters is loaded in advance, to suppress the activity of microorganism in bottle in described Dewar bottle.

In addition, in described step 2) measure in the water sample of described sediment ²⁹n ₂with ³⁰n ₂concentration process in, stirring apparatus need be kept to be in running status all the time.

P is calculated by described indoor mud test ²⁹n ₂and p ³⁰n ₂method as follows: gather original position top layer (0-10cm) sediment fresh sample, 3 grams of fresh samples of sediment loaded in 12mL column vial (Labco, UK), fills oxygen-free water, add when a beaded glass is beneficial to reaction simultaneously and shake up.Then inject 100 μ L 20mM's ¹⁵nO ₃ ^-solution ( ¹⁵n abundance is 99.29%) or ( ¹⁵nH ₄) ₂sO ₄solution ( ¹⁵n abundance is 98.2%).Totally 3 process: 1. only add ¹⁵nH ₄ ⁺; 2. add ¹⁵nH ₄ ⁺with ¹⁴nO ₃ ^-; 3. only add ¹⁵nO ₃ ^-+ 14NH ₄ ⁺.At 0h, 1h, 2h, 4h, 8h, each reaction bulb injects 200 μ L 50% (grams per milliliter) solder(ing)acids respectively with cessation reaction.Then, in anaerobic culture box (YQX-II, Shanghai new talent medicine equipment Manufacturing Co., Ltd), 2mL supernatant is proceeded to another 12mL and be full of in the Labco bottle (sample injection bottle) of He gas.Violent shake Labco bottle 1min, makes the N be dissolved in water sample ₂discharge, then use intestinal bacteria instrument (Gasbench-MAT253) to measure in these 5 time point sample bottles ²⁹n ₂with ³⁰n ₂concentration (unit is nmol N ₂l ^-1), the time is differentiated and obtains ²⁹n ₂with ³⁰n ₂concentration is rate over time ²⁹k and ³⁰(unit is nmol N to K ₂l ^-1h ^-1).And then obtain ²⁹k × (12-2) × 10 ^-3l/ (3g × sediment water percentage) is p ²⁹n ₂(unit is nmol N ₂g ^-1h ^-1); ³⁰k × (12-2) × 10 ^-3l/ (3g × sediment water percentage) is p ³⁰n ₂(unit is nmol N ₂g ^-1h ^-1).

The present invention utilizes ¹⁵the isotopically tagged method of N, within the scope of water/sediment certain space Culture in situ, gather isotropic deposit thing overlying water, thus further mensuration is wherein dissolved ²⁹n ₂with ³⁰n ₂, with the contribution rate calculating water/sediment certain area Anammox speed and remove nitrogen.

This invention has the following advantages:

1, simulate the environmental baselines such as in-situ temperature, illumination, nutrient situation completely, make result of study closer to environment real conditions;

2, adopt one group of stirring apparatus to make overlying water homogeneous, institute's sample thief is representative;

3, the method for sampling is simple, and device is light, convenient operation, has important using value.

Accompanying drawing explanation

Fig. 1 is in-site detecting provided by the invention water/sediment Anammox speed and apparatus structure schematic diagram nitrogen being removed to contribution rate thereof, wherein, 1. without the syringe of piston; 2. T-valve; 3. sampling injector; 4. silicone tube (6 × 4); 5. direct supply (12V); 6. drip-proof motors; 7. transmission shaft; 8. sample glass tube; 9. stirrer; 10. plug; 11. stainless-steel tubes (internal diameter 6.2cm, long 60cm); 12. overlying waters; 13. sediments.

Embodiment

Below in conjunction with specific embodiment, the present invention is further elaborated, but the present invention is not limited to following examples.Described method is conventional method if no special instructions.Described material all can obtain from open commercial sources if no special instructions.

Embodiment 1, in-site detecting Tianjin Qilihai wetland water/sediment Anammox speed and the device to nitrogen removal contribution rate thereof

The structural representation of this device as shown in Figure 1, comprises the stainless-steel tube 11 of open at both ends, the plug 10 mated with stainless-steel tube 11 and the accessory of disposed therethrough on plug 10;

Plug 10 and stainless-steel tube 11 seal completely;

Accessory on plug comprises pressure adjustment assembly, stirring apparatus and sampler;

Wherein, stirring apparatus comprises the drip-proof motors 6, transmission shaft 7 and the stirrer 9 that connect in turn; Drip-proof motors 6 is positioned at the center of plug 10, and through plug 10; Transmission shaft 7 and stirrer 9 are positioned at pipe 11;

Drip-proof motors 6 seals completely with the joint glass cement of plug 10;

Sampler comprises the sampling injector 3, T-valve 2, silicone tube 4 and the sampling glass tube 8 being positioned at stainless-steel tube 11 that connect in turn from top to bottom.

Wherein, pressure adjustment assembly is preferably the syringe 1 without piston, and the effect of this pressure adjustment assembly ensures the air pressure balance inside and outside stainless-steel tube.In use, need first by original position water fully with the syringe 1 reinjected after high-pure helium aeration without piston.

Stainless-steel tube 11 has higher-strength, can ensure that it inserts sediment certain depth to be measured.Plug 10 can seal completely with stainless-steel tube 11, ensures this columnar water/sediment with extraneous without mass exchange.

The object of T-valve 2 and a sampling injector 3 that the two ends of silicone tube 4 connect a Quality control collection capacity be respectively ensure with extraneous without the condition of mass exchange under overlying water sample in quantitative collection cylinder.

Measure Anammox speed and the power supply 5 be connected with drip-proof motors 6 is also comprised, to provide drip-proof motors 6 required drive to the device of nitrogen removal contribution rate.The transmission shaft 7 be connected with drip-proof motors 6 and stirrer 9 are for stirring and evenly mixing sediment overlying water.In addition, drip-proof motors 6 seals with the joint glass cement of plug 10, to ensure impermeability.

Embodiment 2, in-site detecting Tianjin Qilihai wetland water/sediment Anammox speed and the contribution rate that nitrogen is removed thereof

1) in Qilihai wetland land inland water ecotone region, Tianjin, select the research place of the suitable overlying water degree of depth, stainless-steel tube 11 (internal diameter 6.2cm, long 60cm) is inserted sediment 40cm, stainless-steel tube 11 is uncovered stablize 1 day after, add 1.7mL, 200mM ¹⁵nO ₃ ^-aqueous solution (abundance F _nbe 99.29%), make NO in cylinder overlying water ₃ ^--N concentration reaches 7.89mg L ^-1.Then syringe (also namely without the syringe of piston) 1 (20mL), drip-proof motors 6 (model: MPM-060BS-12C-298I-3.039 is regulated installing pressure; Specification: 4.0cm × 2.0cm × 1.5cm), sampling glass tube 8 (internal diameter 0.2cm, long 22cm), (specification is No. 13, upper diameter: 6.5cm for the plug 10 of silicone tube 4 (specification: 6cm × 4cm), T-valve 2 and sampling injector 3; Diameter below: 5.5cm; High: 4.0cm) fill in stainless-steel tube 11 upper end, surrounding band seals.In addition, this plug 10 above center opens the longitudinal hole of a diameter 3cm, inserts a drip-proof motors 6, the hollow Stainless Steel Transmission Shaft 7 (long 10cm, internal diameter 2mm) of motor shaft lower end welding one, and the long stirrer 9 of a 2cm is fixed in transmission shaft lower end.

2) switch on power, after stirring (rotating speed is 60rpm) 20min, first prefabricated oxygen-free water (by original position water fully with high-purity He aeration) injection pressure is regulated syringe (also namely without the syringe of piston) 1, after ensureing that this pillar-shaped voxel and the external world do not have air exchange, air in sampling glass tube 8 is extracted out, then extract 12mL water sample out and slowly inject 12mL Labco Dewar bottle (bottle prior loading 0.2mL 50% (grams per milliliter) solder(ing)acid, for suppressing microbial activity), measure in the water sample of this system of different time points ²⁹n ₂with ³⁰n ₂concentration, and then according to ²⁹n ₂with ³⁰n ₂concentration, calculate the contribution rate that Anammox speed and Anammox are removed nitrogen.

Wherein, is opened the water sample that this T-valve (2) extracts this system out first time and be designated as first time point in the time point of Dewar bottle, this sample is as the sample of first time point (0h), again in the same way respectively at 12h, 24h, 36h, 48h gather water sample, measure in the water sample of 12h, 24h, 36h, 48h sediment ²⁹n ₂with ³⁰n ₂concentration; Stirring apparatus is kept to be in running status (60rpm) in this process.

According to this ²⁹n ₂with ³⁰n ₂concentration calculate this Anammox speed and the method for Anammox to the contribution rate that nitrogen is removed comprises the steps: in the water sample according to this system during gained different time points ²⁹n ₂with ³⁰n ₂concentration, calculate in this sediment unit area ²⁹n ₂with ³⁰n ₂generation speed, be respectively p ²⁹n ₂ ^in-situand p ³⁰n ₂ ^in-situ, by this p ²⁹n ₂ ^in-situand p ³⁰n ₂ ^in-situsubstitution formula I and formula II, obtains ²⁸n ₂, ²⁹n ₂with ³⁰n ₂total generation speed TN ₂p, Anammox produce ²⁹n ₂with ³⁰n ₂generation speed Anmx, to nitrogen remove contribution rate Ra ^in-situ:

Ra ^in-situ＝Anmx/TN ₂P

TN ₂p=2r ₁₄[p ²⁹n ₂ ^{in situ}+ p ³⁰n ₂ ^{in situ}(1-r ₁₄)] formula I

Anmx=2r ₁₄(p ²⁹n ₂ ^{in situ}-2r ₁₄p ³⁰n ₂ ^{in situ}) formula II

This p ²⁹n ₂ ^in-situand p ³⁰n ₂ ^in-situunit be nmol N ₂m ^-2h ^-1; This TN ₂the unit of P and Anmx is nmol N m ^-2h ^-1.

In this formula I and formula II, r ₁₄for in reduction film micro area ¹⁴nO ₃ ^-with ¹⁵nO ₃ ^-ratio, r ₁₄calculated by formula III and obtain:

$r_{14}=\frac{(1-\mathrm{Ra})\left(\frac{p^{29}{N}_2}{p^{30}{N}_2}\right)-\mathrm{Ra}}{(2-\mathrm{Ra})}$ Formula III

In this formula III, p ²⁹n ₂, p ³⁰n ₂, Ra by formula IV to formula VI calculate and obtain:

$D_{\mathrm{total}}=p^{30}{N}_2\×F_N^{-2}$ Formula IV

$A_{\mathrm{total}}=F_N^{-1}\×[p^{29}{N}_2+2\×(1-F_N^{-1})\×p^{30}{N}_2]$ Formula V

$\mathrm{Ra}=\frac{A_{\mathrm{total}}}{A_{\mathrm{total}}+D_{\mathrm{total}}}\×100\%$ Formula VI

In the full formula VI of this formula IV,

D _totalfor being produced by denitrification ²⁸n ₂, ²⁹n ₂with ³⁰n ₂speed, unit is nmol N ₂g ^-1h ^-1;

A _totalfor being produced by Anammox ²⁸n ₂with ²⁹n ₂speed, unit is nmol N ₂g ^-1h ^-1;

Ra is for produce N by Anammox ₂ratio;

P ²⁹n ₂for in indoor mud test ²⁹n ₂generation speed, unit is nmol N ₂g ^-1h ^-1;

P ³⁰n ₂for in indoor mud test ³⁰n ₂generation speed, unit is nmol N ₂g ^-1h ^-1;

F _nfor in indoor mud test nitrate used ¹⁵the abundance (being 99.29% in this embodiment) of N.

Concrete computation process is as follows:

(1) N is passed through ₂generation can obtain the regretional analysis of time, original position simulation post in ²⁹n ₂with ³⁰n ₂generation speed be respectively: 26.815nmol h ^-1with 171.062nmol h ^-1.

Consider stainless-steel tube section radius R=3.1cm, water/sediment unit area N ₂generation speed be:

P ²⁹n ₂ ^in-situ=8.886 μm of ol N ₂m ^-2h ^-1and p ³⁰n ₂ ^in-situ=56.689 μm of ol N ₂m ^-2h ^-1.

(2) by p ²⁹n ₂=0.896nmol N ₂g ^-1h ^-1and p ³⁰n ₂=0.117nmol N ₂g ^-1h ^-1substitution formula (III), (IV), (V) and (VI), can obtain:

r ₁₄＝0.00715。

(3) by p ²⁹n ₂ ^in-situ=8.886 μm of ol N ₂m ^-2h ^-1, p ³⁰n ₂ ^in-situ=56.689 μm of ol N ₂m ^-2h ^-1and r ₁₄=0.00715 substitutes into (I) and (II) can obtain:

The N generation speed TN that original position is total ₂p=0.932 μm of ol N m ^-2h ^-1;

Original position Anammox produces the speed Anmx=0.115 μm of ol N m of N ^-2h ^-1;

Anammox effect is to N ₂the contribution rate Ra removed ^in-situ=Anmx/TN ₂p=12.4%.

Claims (7)

1. in-site detecting water/sediment Anammox speed and the device to nitrogen removal contribution rate thereof, comprise the pipe (11) of open at both ends, the plug (10) mated with described pipe (11) and the accessory of disposed therethrough on described plug (10);

Described plug (10) and described pipe (11) seal completely; Described pipe (11) is stainless-steel tube;

Accessory on described plug comprises pressure adjustment assembly, stirring apparatus and sampler;

Wherein, described stirring apparatus comprises the drip-proof motors (6), transmission shaft (7) and the stirrer (9) that connect in turn; Described drip-proof motors (6) is positioned at the center of described plug (10), and through described plug (10); Described transmission shaft (7) and stirrer (9) are positioned at described pipe (11);

Described drip-proof motors (6) seals completely with the joint glass cement of described plug (10);

Described drip-proof motors (6) is connected with power supply (5); Described power supply (5) is direct supply;

Described sampler comprises the sampling injector (3), T-valve (2), silicone tube (4) and the sampling glass tube (8) being positioned at described pipe (11) that connect in turn from top to bottom;

Described pressure adjustment assembly is the syringe (1) without piston.

2. in-site detecting water/sediment Anammox speed and the method to nitrogen removal contribution rate thereof, comprise the steps:

1) pipe (11) in device described in claim 1 is inserted water to be measured/sediment 30-50cm stable after, add in the water of described pipe (11) ¹⁵nO ₃ ^-the aqueous solution of salt, again the plug (10) in device described in claim 1 and the accessory on described plug (10) are all filled in the upper end of described pipe (11), make the lower end of described sampling glass tube (8) apart from described water to be measured/3-8cm place, sediment interface, and seal the gap of described plug (10) and described pipe (11);

2) after utilizing described stirring apparatus to stir, oxygen-free water is injected described pressure adjustment assembly, open described T-valve (2), after extracting the air in described sampling glass tube (8) out with described sampling injector (3), close described T-valve (2), pull out described sampling injector (3), again described sampling injector (3) is inserted in described T-valve (2) after draining air, open described T-valve (2) and extract the water sample of described water/sediment to be measured out in Dewar bottle, measure in the water sample of water/sediment described in different time points ²⁹n ₂with ³⁰n ₂concentration, and then according to described ²⁹n ₂with ³⁰n ₂concentration, calculate the contribution rate that described Anammox speed and Anammox are removed nitrogen,

Measure in the water sample of water/sediment described in different time points ²⁹n ₂with ³⁰n ₂the method of concentration comprise the steps:

Is opened the water sample that described T-valve (2) extracts described water/sediment to be measured out first time and be designated as first time point in the time point of Dewar bottle, measure in the water sample of described water/sediment during this first time point ²⁹n ₂with ³⁰n ₂concentration, be respectively ²⁹c ₁with ³⁰c ₁, then when measuring 1+n time point in the same way respectively described water/sediment water sample in ²⁹n ₂with ³⁰n ₂concentration, be respectively ²⁹c _1+nwith ³⁰c _1+n, wherein, n is the integer of 1-5; Adjacent two time points be spaced apart 8-16 hour;

Described in described basis ²⁹n ₂with ³⁰n ₂concentration calculate described Anammox speed and the method for Anammox to the contribution rate that nitrogen is removed comprises the steps:

In water sample according to water/sediment described during gained different time points ²⁹n ₂with ³⁰n ₂concentration, calculate in described water/sediment unit area ²⁹n ₂with ³⁰n ₂generation speed, be respectively p ²⁹n ₂ ^in-situand p ³⁰n ₂ ^in-situ, by described p ²⁹n ₂ ^in-situand p ³⁰n ₂ ^in-situsubstitution formula I and formula II, obtains ²⁸n ₂, ²⁹n ₂with ³⁰n ₂total generation speed TN ₂p, Anammox produce ²⁹n ₂with ³⁰n ₂speed Anmx, Anammox contribution rate Ra that nitrogen is removed ^in-situ:

Ra ^in-situ＝Anmx/TN ₂P

TN ₂p=2r ₁₄[p ²⁹n ₂ ^in-situ+ p ³⁰n ₂ ^in-situ(1-r ₁₄)] formula I

Anmx=2r ₁₄(p ²⁹n ₂ ^in-situ-2r ₁₄p ³⁰n ₂ ^in-situ) formula II

Described p ²⁹n ₂ ^in-situand p ³⁰n ₂ ^in-situunit be nmol N ₂m ^-2h ^-1; Described TN ₂the unit of P and Anmx is nmol N m ^-2h ^-1;

In described formula I and formula II, r ₁₄for in reduction film micro area ¹⁴nO ₃ ^-with ¹⁵nO ₃ ^-ratio, r ₁₄calculated by formula III and obtain:

$r_{14}=\frac{(1-\mathrm{Ra})\left(\frac{p^{29}{N}_2}{p^{30}{N}_2}\right)-\mathrm{Ra}}{(1-\mathrm{Ra})}$ Formula III

In described formula III, p ²⁹n ₂, p ³⁰n ₂, Ra by formula IV to formula VI calculate and obtain:

$D_{\mathrm{total}}=p^{30}{N}_2\×F_N^{-2}$ Formula IV

$A_{\mathrm{total}}=F_N^{-1}\×(p^{29}{N}_2+2\×(1-F_N^{-1})\×p^{30}{N}_2)$ Formula V

$\mathrm{Ra}=\frac{A_{\mathrm{total}}}{A_{\mathrm{total}}+D_{\mathrm{total}}}\×100\%$ Formula VI

Described formula IV in formula VI,

D _totalfor being produced by denitrification ²⁸n ₂, ²⁹n ₂with ³⁰n ₂speed, unit is nmol N ₂g ^-1h ^-1;

A _totalfor being produced by Anammox ²⁸n ₂with ²⁹n ₂speed, unit is nmol N ₂g ^-1h ^-1;

Ra is for produce N by Anammox ₂ratio;

P ²⁹n ₂for in indoor mud test ²⁹n ₂generation speed, unit is nmol N ₂g ^-1h ^-1;

P ³⁰n ₂for in indoor mud test ³⁰n ₂generation speed, unit is nmol N ₂g ^-1h ^-1;

F _nfor in indoor mud test nitrate used ¹⁵the abundance of N.

3. method according to claim 2, is characterized in that: described step 2) in, in the water sample of water/sediment described in described mensuration different time points ²⁹n ₂with ³⁰n ₂the pre-treating method of concentration be helium Head space.

4. method according to claim 3, is characterized in that: described step 1) in, described in ¹⁵nO ₃ ^-the consumption of the aqueous solution of salt for make described pipe (11) water in NO ₃ ^-the concentration of-N is 5-10mg L ^-1.

5. method according to claim 4, is characterized in that: described step 2) in described whipping step, mixing time is 15-30min, and speed is 50-70rpm.

6. method according to claim 5, is characterized in that: the solder(ing)acid loading 0.2mL 50% (g/mL) in described Dewar bottle in advance.

7. method according to claim 2, is characterized in that, described step 2) in, described n is 4; Described adjacent two time points be spaced apart 12 hours.