CN102559772A - Method for increasing production of coal bed methane by using exogenous microorganisms - Google Patents
Method for increasing production of coal bed methane by using exogenous microorganisms Download PDFInfo
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- CN102559772A CN102559772A CN2012100356820A CN201210035682A CN102559772A CN 102559772 A CN102559772 A CN 102559772A CN 2012100356820 A CN2012100356820 A CN 2012100356820A CN 201210035682 A CN201210035682 A CN 201210035682A CN 102559772 A CN102559772 A CN 102559772A
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
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Abstract
The invention belongs to the field of increasing production of coal bed methane through microorganisms, and relates to a method for increasing production of coal bed methane by using exogenous microorganisms. The method is characterized by comprising the following steps of: performing fermentation culture of microorganisms for increasing production of the coal bed methane in a fermentation reaction tank of a ground plant, and injecting a fermentation liquor (the microorganisms and nutrients) into a coal bed. The method has the advantages that: the fermentation is carried out on the ground, the fermentation liquor is injected into the coal bed at high pressure by drilling in a microorganism growth index period, and a hole is sealed for 35 to 40 days; exogenous bacteria are used for activating indigenous bacteria in the coal bed, organic matters on the surface of coal are degraded to form methane gas; the nutrient solution is injected to provide starting energy for the metabolism of the microorganisms, and methane production lag phase time can be shortened. The method is easy to implement, is influenced by environment a little and has high adaptability.
Description
Technical field
The invention belongs to mikrobe raising the output coal-seam gas field, relate to a kind of mikrobe raising the output coal-seam gas method of utilizing.
Background technology
Thereby mikrobe raising the output coal-seam gas is to utilize Metabolic activity degraded coal surface organic matter and/or the dissolved organic matters such as meta-bolites such as organic acid, alcohol of mikrobe self to increase the coal seam porosity; Improve rate of permeation; Final methane concentration in the coal-seam gas, the raising extraction amount of improving.
The U.S. proposed microbial augmentation coal-bed gas exploitation (MECR) in 1999 first; Mainly be through in the coal seam, injecting nutritive medium and/or bacterial classification; Make bacterial classification breeding in a large number in the coal seam, thus the methane gas of desorb coal seam surface adsorption and utilize the organic methane gas that generates in coal surface to increase a ton coal gas production rate.The U.S. was applied to basin, powder river with this technology in 2004.Canada, Australia etc. also take much count of the research to MECR, and are applied to the coal seam to achievement in research.Compare with conventional coal-seam gas raising the output method, mikrobe raising the output coal-seam gas causes the attention of various countries coal-bed gas exploitation company with its considerable economic, unique advantage and vast potential for future development.After the nineties in 20th century, China also begins mikrobe raising the output coal-seam gas Study on Technology and application work.Some colleges and universities such as China Mining University, the Chinese Academy of Sciences, University of Science & Technology, Beijing etc. carry out the research of China's biogenesis coal-seam gas in succession; Like the Caidamu Basin, basin, the Baoshan, Yunnan, Anhui Xin Ji coal-seam gas etc.; Carried out a large amount of fundamental research work, simultaneously also in bacterial screening that carries out mikrobe raising the output coal-seam gas and relevant shop experiment.
Summary of the invention
The purpose of this invention is to provide a kind of concrete mikrobe raising the output coal-seam gas method, the construction of this kind method is simple, and environmental influence is changed not quite, and flexibility is strong.
For realizing above-mentioned purpose, the technological step that the present invention takes is following:
(1) enrichment of external source bacterial classification and domestication.Enrichment culture is carried out in sampling from environment such as municipal wastewater treatment plant anaerobic digestor or sludge digestion tank or river anaerobic bottom mud; Mixing anaerobic flora after the enrichment culture is through further adapting to the domestication of coal mine environment; And definite (constituent content: K:0.004-0.008 mol/L, P:0.005-0.009 mol/L, N:0.015-0.020 mol/L, Mg:0.010-0.020 mol/L, Na:0.012-0.018 mol/L) of nutrient composition proportioning; Carry out the test (methane concentration, voltaile fatty acid in the gas) and the strain identification of physiological and biochemical index (microorganism growth cycle, sulphate reducing bacteria) and meta-bolites at last, finally obtain needed bacterial classification.Wherein the detection method of physiological and biochemical index and meta-bolites is following: 1) the microorganism growth cycle: adopt spectrophotometric determination.Adaptive phase: 1-15 days, logarithmic phase: 12-35 days, stationary phase: 30-55 days, decline phase: 50-60 days; 2) sulphate reducing bacteria: through measuring H in the gas that produces
2S concentration is divided into 4 ranks, I: 0 ~ 200ppm; II: 200 ~ 1000ppm; III: 1000 ~ 1500ppm; IV:>1500ppm.Because sulphate reducing bacteria and methanogen competition nutritive substance, therefore H in the gas that produces
2S concentration methanogen activity in the I scope is the highest, and this moment, gas production rate was maximum.The measuring method of meta-bolites is: 1) methane concentration in the gas: adopt gas chromatograph for determination CH
4Concentration, CH in gas
4Concentration greater than 80%, CO
2Can adopt scope less than 20% for economical and effective.2) voltaile fatty acid: adopt high performance liquid chromatograph to measure.Wherein mainly detecting index is acetate, and content is 4 ~ 14g/L.
(2) fermentation of external source bacterial classification and quantity amplification.In surface facility fermentation reaction jar, carry out the inoculating microbe fermentation, amplification bacterial classification quantity improves microorganism active.
(3) during cultivating above-mentioned external source bacterium, adopt molecular biology method that quantity and the activity of the endogenous bacterium in the coal seam (claiming the origin bacterium again) are measured.
(4) external source bacterial classification injection coal seam: the method that the boring of employing high pressure is injected is injected the coal seam with the fermented liquid of surface facility fermentor tank, and sealing of hole was 30-40 days after injection finished.Detect the variation and the biological community structure of organic acid content in the interior methane concentration of boring, the pit water during this time.Wherein, methane concentration adopts portable methane determinator to measure; Organic acid content adopts high-performance liquid chromatogram determination in the pit water; Biological community structure adopts the PCR-DGGE technical measurement.
(5) concentration of methane gas reaches economy and can the value of adopting can carry out the coal-seam gas extraction.
The shallow overburden coal seam possesses the suitable condition of microorganism growth, but the low air content that causes of origin bacterium activity and quantity is not enough, and the injection of external source methanogen can activate the origin bacterium in coal seam.The present invention is in the fermentation reaction jar of surface facility, and fermentation culture is increased production the coal-seam gas inoculating microbe, and fermented liquid (mikrobe and nutritive substance) is injected the coal seam.The advantage of this method is to ferment on ground, and injects the coal seam in the microorganism growth logarithmic phase through the boring high pressure, and sealing of hole was 30-40 days after injection finished.Utilize the external source bacterium to activate the origin bacterium in the coal seam, the organic methane gas that generates in degraded coal surface; The injection of nutritive medium provides the startup energy to microbial metabolism, has shortened aerogenesis retardation time.
Description of drawings
Fig. 1 is external source bacterium in the embodiment of the invention 1,2,3, origin bacterium and mixes bacterium simulation aerogenesis experimental result.
Embodiment
Below in conjunction with embodiment the present invention is further described:
(1) the external source bacterium is taken from municipal wastewater treatment plant anaerobic digestor or sludge digestion tank or river anaerobic bottom mud; Mud is inserted the enrichment of methanogen enrichment medium; Get supernatant after 25-30 days and be inoculated into enrichment once more in the new enrichment medium; Inoculate 2-3 time so repeatedly, again pregnant solution is inoculated in the no carbon source substratum and adds a certain amount of being crushed to until aerogenesis is stable, get supernatant after 25-30 days and be inoculated in the new substratum and tame less than 0.074mm coal sample acclimation shaking culture; The add-on of coal constantly increases in the domestication process; Tame 7-8 back mikrobe and can utilize the coal aerogenesis, and the aerogenesis time shortened to 5-8 days by 15-20 days, it is 0.86-0.66m that the ton coal produces a methane content
3/ t. days.
Mikrobe after the domestication is the mixture of external source bacterial classification, consists of: hydrolysed ferment bacterium, product hydrogen acetogen, cellulose-decomposing bacterium, methanogen.Therefore the methanogen coal of can not directly degrading needs zymogenic bacteria, produces the hydrogen acetogen, cellulose-decomposing bacterium is decomposed into the material that acetate, formic acid, carbonic acid gas etc. can be utilized by methanogen with coal surface larger molecular organics earlier.The principle and the process of its raising the output coal-seam gas are following:
(2) in the fermentation reaction jar of surface facility, carry out multiplication culture in the inoculating microbe adding fermentor tank that nutritive medium and domestication is good.Each elemental composition is in the nutritive medium: (mol/L):
K:0.004-0.008、P:0.005-0.009、N:0.015-0.020、Mg:0.010-0.020、Na:0.012-0.018
(3) origin methanogen floras in difference enrichment coal seam and the pit water: the different depths coal sample is got in boring; With core pack into rapidly the sealing analytic tube in; Methane gas not had is separated out the back and under oxygen free condition, it is crushed to certain fineness, adds to simulate the aerogenesis experiment in the serum bottle that aseptic methanogen enrichment medium is housed; Get pit water with aseptic anaerobic container, under oxygen free condition, be inoculated in the serum bottle that aseptic product methane substratum and sterilization coal are housed and simulate the aerogenesis experiment.Carry out the test of physiological and biochemical index and meta-bolites during the aerogenesis, carry out strain identification at last, adopt the sampling of PCR-DGGE technology to carry out the analysis of flora quantity and structure.
(4) external source bacterium entering logarithmic growth after date in the fermentor tank of ground, the method that adopts high pressure boring to inject is injected the coal seam with fermented liquid, and sealing of hole was 30-40 days after injection finished.Detect the variation and the biological community structure of organic acid content in the interior methane concentration of boring, the pit water during this time.Wherein, methane concentration adopts portable methane determinator to measure; Organic acid content adopts high-performance liquid chromatogram determination in the pit water; Adopt PCR-DGGE technical Analysis biological community structure, confirm the different aerogenesis dominant microflora in period.
(5) concentration of methane gas reaches economy and can the value of adopting can carry out the coal-seam gas extraction.
Embodiment 1: the external source bacterium utilizes the experiment of coal aerogenesis
According to summary of the invention configuration 15-20ml nutritive medium, under oxygen free condition, add the 5-10g granularity after the sterilization deoxygenation less than the aseptic coal of 0.074mm, inoculation 2-4ml external source fermented liquid is put into 33-38 ℃ of incubator and is simulated the aerogenesis experiment after the sealing.
Embodiment 2: the origin bacterium utilizes the experiment of coal aerogenesis
According to summary of the invention 2 configuration 15-20ml nutritive mediums, under oxygen free condition, add the 5-10g granularity after the sterilization deoxygenation less than the aseptic coal of 0.074mm, inoculation 2-4ml coal mine water is put into 32-38 ℃ of incubator and is simulated the aerogenesis experiment after the sealing.
Embodiment 3: external source bacterium and origin bacterium are united and utilize coal aerogenesis experiment
According to summary of the invention 2 configuration 15-20ml nutritive mediums; Under oxygen free condition, add the 6-10g granularity after the sterilization deoxygenation less than the aseptic coal of 0.074mm; Inoculation 0.5-1ml external source fermented liquid and 0.6-1ml coal mine water are put into 32-37 ℃ of incubator and are simulated the aerogenesis experiment after the sealing.
Claims (2)
1. one kind is utilized inoculating microbe raising the output coal-seam gas method, it is characterized in that the concrete steps of this method are:
(1) enrichment of bacterial classification and domestication: enrichment culture is carried out in sampling from environment such as municipal wastewater treatment plant anaerobic digestor or sludge digestion tank or river anaerobic bottom mud; Mixing anaerobic bacterial classification after the enrichment culture is through the further domestication of adaptation coal mine environment and confirming of nutrient composition proportioning; Its constituent content proportioning and scope are: K:0.004-0.008 mol/L, P:0.005-0.009 mol/L, N:0.015-0.020 mol/L, Mg:0.010-0.020 mol/L, Na:0.012-0.018 mol/L; Carry out the test and the strain identification of physiological and biochemical index and meta-bolites again; The final external source bacterial classification that obtains, the external source bacterial classification consists of: hydrolysed ferment bacterium, product hydrogen acetogen, cellulose-decomposing bacterium and methanogen; Wherein the detection method of physiological and biochemical index and meta-bolites is following: 1) the microorganism growth cycle: adopt spectrophotometric determination; Adaptive phase: 1-15 days, logarithmic phase: 12-35 days, stationary phase: 30-55 days, decline phase: 50-60 days; 2) sulphate reducing bacteria: through measuring H in the gas that produces
2S concentration is divided into 4 ranks, I: 0 ~ 200ppm; II: 200 ~ 1000ppm; III: 1000 ~ 1500ppm; IV:>1500ppm; Because sulphate reducing bacteria and methanogen competition nutritive substance, therefore H in the gas that produces
2S concentration methanogen activity in the I scope is the highest, and this moment, gas production rate was maximum; The measuring method of meta-bolites is: 1) methane concentration in the gas: adopt gas chromatograph for determination CH
4Concentration, CH in gas
4Concentration greater than 80%, CO
2Can adopt scope less than 20% for economical and effective; 2) voltaile fatty acid: adopt high performance liquid chromatograph to measure; Wherein mainly detecting index is acetate, and content is 4 ~ 14g/L;
(2) fermentation of external source bacterial classification and quantity amplification: in surface facility fermentation reaction jar, carry out microbial fermentation, amplification bacterial classification quantity improves microorganism active;
(3) during cultivating the external source bacterial classification, adopt molecular biology method that endogenous bacterium quantity and activity in the coal seam are measured;
(4) external source bacterial classification injection coal seam: the method that adopts high pressure boring to inject is injected the coal seam with fermented liquid, and sealing of hole was 35-40 days after injection finished; Detect the variation of variation, microbial population, quantity and the structure of organic acid content in methane concentration, the pit water in the boring during this time;
(5) concentration of methane gas reaches economy and can the value of adopting can carry out the coal-seam gas extraction.
2. a kind of inoculating microbe raising the output coal-seam gas method of utilizing as claimed in claim 1 is characterized in that in the step (4) that methane concentration adopts portable methane determinator to measure; Organic acid content adopts high-performance liquid chromatogram determination in the pit water; Adopt PCR-DGGE technical Analysis biological community structure, confirm the different aerogenesis dominant microflora in period.
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CN105462908A (en) * | 2014-08-29 | 2016-04-06 | 肖栋 | Technology and method for rapid degradation of organic matters by using microbial community in coal seam |
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CN105462908A (en) * | 2014-08-29 | 2016-04-06 | 肖栋 | Technology and method for rapid degradation of organic matters by using microbial community in coal seam |
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Application publication date: 20120711 |