CN106285581A - A kind of method utilizing origin bacterium to improve methane output - Google Patents
A kind of method utilizing origin bacterium to improve methane output Download PDFInfo
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- CN106285581A CN106285581A CN201610710769.1A CN201610710769A CN106285581A CN 106285581 A CN106285581 A CN 106285581A CN 201610710769 A CN201610710769 A CN 201610710769A CN 106285581 A CN106285581 A CN 106285581A
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- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 title claims abstract description 138
- 238000000034 method Methods 0.000 title claims abstract description 69
- 241000894006 Bacteria Species 0.000 title claims abstract description 44
- 239000003245 coal Substances 0.000 claims abstract description 120
- 238000004519 manufacturing process Methods 0.000 claims abstract description 23
- 238000000855 fermentation Methods 0.000 claims abstract description 21
- 230000004151 fermentation Effects 0.000 claims abstract description 18
- 230000008569 process Effects 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 230000008859 change Effects 0.000 claims abstract description 10
- 238000004458 analytical method Methods 0.000 claims abstract description 8
- 230000015556 catabolic process Effects 0.000 claims abstract description 6
- 238000006731 degradation reaction Methods 0.000 claims abstract description 6
- 238000005065 mining Methods 0.000 claims abstract description 6
- 238000001514 detection method Methods 0.000 claims abstract description 5
- 230000000813 microbial effect Effects 0.000 claims abstract description 3
- 239000007789 gas Substances 0.000 claims description 32
- 238000012545 processing Methods 0.000 claims description 25
- 230000000844 anti-bacterial effect Effects 0.000 claims description 22
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 19
- 238000002474 experimental method Methods 0.000 claims description 19
- 229910052739 hydrogen Inorganic materials 0.000 claims description 19
- 239000001257 hydrogen Substances 0.000 claims description 19
- 238000013480 data collection Methods 0.000 claims description 16
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 15
- 239000011573 trace mineral Substances 0.000 claims description 14
- 235000013619 trace mineral Nutrition 0.000 claims description 14
- 239000001963 growth medium Substances 0.000 claims description 11
- 238000004891 communication Methods 0.000 claims description 9
- 244000005700 microbiome Species 0.000 claims description 8
- 238000009210 therapy by ultrasound Methods 0.000 claims description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- 239000002609 medium Substances 0.000 claims description 6
- 230000002906 microbiologic effect Effects 0.000 claims description 6
- 238000005516 engineering process Methods 0.000 claims description 4
- 239000011148 porous material Substances 0.000 claims description 4
- 238000000746 purification Methods 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 238000012360 testing method Methods 0.000 claims description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 3
- 238000011529 RT qPCR Methods 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 229910052799 carbon Inorganic materials 0.000 claims description 3
- 239000007943 implant Substances 0.000 claims description 3
- 230000002401 inhibitory effect Effects 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 238000004445 quantitative analysis Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 230000008878 coupling Effects 0.000 claims description 2
- 238000010168 coupling process Methods 0.000 claims description 2
- 238000005859 coupling reaction Methods 0.000 claims description 2
- 230000002349 favourable effect Effects 0.000 claims description 2
- 230000007062 hydrolysis Effects 0.000 claims description 2
- 238000006460 hydrolysis reaction Methods 0.000 claims description 2
- 235000015097 nutrients Nutrition 0.000 claims description 2
- 238000002604 ultrasonography Methods 0.000 description 8
- 238000000605 extraction Methods 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 241000192125 Firmicutes Species 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 241000205276 Methanosarcina Species 0.000 description 2
- 150000001335 aliphatic alkanes Chemical class 0.000 description 2
- 239000007640 basal medium Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000006065 biodegradation reaction Methods 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000000696 methanogenic effect Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 231100000167 toxic agent Toxicity 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241001156739 Actinobacteria <phylum> Species 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 241000205017 Methanolobus Species 0.000 description 1
- PLXBWHJQWKZRKG-UHFFFAOYSA-N Resazurin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3[N+]([O-])=C21 PLXBWHJQWKZRKG-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical class OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 229910052927 chalcanthite Inorganic materials 0.000 description 1
- 239000003034 coal gas Substances 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 208000028659 discharge Diseases 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 229910052603 melanterite Inorganic materials 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 230000011218 segmentation Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
Classifications
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/006—Production of coal-bed methane
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
- E21B43/164—Injecting CO2 or carbonated water
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention provides a kind of method utilizing origin bacterium to improve methane output, including enrichment and the cultivation of (1) methanogen group: from target area samples of coal pulled and water sample, carry out enrichment culture and the fermentation of methanogen group, obtain fermentation liquid；(2) ultrasonic wave-coupled supercritical CO2Process: utilize laboratory operation management system, at lab analysis ultrasonic wave-coupled supercritical CO2Process the optimum condition of coal seam sample, and at optimum conditions target coal seam is processed；(3) microbial degradation coal: the fermentation liquid described step (1) obtained injects described target coal seam, and persistently well head gas component and methane concentration change are injected in detection, and utilize the mode of drainage and step-down mining to collect methane.The present invention utilizes ultrasonic wave-coupled supercritical CO2Process is effectively shortened the process time, accelerates coal bed gas gas production rate, utilizes laboratory operation management system to be effectively increased work efficiency simultaneously.
Description
Technical field
The present invention relates to improve methane output technical field, a kind of utilize origin bacterium to improve methane output
Method.
Background technology
Coal bed gas has the most carried out exploration and development in coal basin, high, medium and low rank, but low-order coal air content is low, the infiltration of high-order coal
The present situation that rate is low seriously constrains the development of coal gas industry.Utilize microorganism to increase production, in coal seam, i.e. inject the micro-life of methane phase
Thing group, the organic matter in degraded coal, increase the content of methane, and the hole improving coal seam by consuming the substrate in coal is tied
Structure, improves permeability, the yield of coal bed gas can be greatly improved.
The patent of Patent No. 201410363562.2 discloses a kind of method improving coal bed gas recovery ratio, is specially profit
With coal seam in-situ microorganism species and supercritical CO2Pretreatment coal seam, improves coal bed gas recovery ratio, but supercritical CO2Process
Time is longer, and long-term work energy consumption is higher and needs manpower comparing relatively, determines optimum experimental condition, inefficiency.
In view of drawbacks described above, creator of the present invention obtains the present invention finally through research for a long time and practice.
Summary of the invention
For solving above-mentioned technological deficiency, the technical solution used in the present invention is, it is provided that one utilizes origin bacterium to improve coal
The method of layer gas yield, comprises the following steps:
(1) enrichment of methanogen group and cultivation: from target area samples of coal pulled and water sample, carry out methanogen group
Enrichment culture and fermentation, obtain fermentation liquid；
(2) ultrasonic wave-coupled supercritical CO2Process: utilize laboratory operation management system, at lab analysis ultrasound wave
Coupling supercritical CO2Process the optimum condition of coal seam sample, and at optimum conditions target coal seam is processed；
(3) microbial degradation coal: the fermentation liquid described step (1) obtained injects described target coal seam, persistently detects note
Enter well head gas component and methane concentration change, and utilize the mode of drainage and step-down mining to collect methane.
It is also preferred that the left the laboratory operation management system in described step (2) includes server, data collection module, data
Processing module and control module；
Described data collection module, described data processing module and described control module are respectively with described server communication even
Connect；
Described data collection module is connected with described data processing module communication, is used for gathering that described ultrasonic wave-coupled is super faces
Boundary CO2Processing the experiment parameter of coal seam sample, described experiment parameter includes that temperature, pressure, time and unit mass coal are biological
The methane content of degraded output；
Described data processing module is connected with described control module communication, is used for analyzing described data collection module and collects
Information, calculate optimal experiment parameter, i.e. unit mass coal and be biodegradable the temperature in the methane content of output most moment
Degree, pressure and time；
Described control module, is used for arranging ultrasonic wave-coupled supercritical CO2Process the experiment parameter of coal seam sample and show
Show the analysis result of described data processing module.
It is also preferred that the left described step (1) comprises the following steps:
I. being sampled at coal bed gas well head, sample bottle sterilizing in advance and full nitrogen, water sample takes full empty to nothing in bottle
Gas remains, and sends in anaerobic culture box within 24 hours in the way of shading；
II. with target coal seam as carbon source, from described step I output water sample, cultivate microorganism species therein, enrichment training
Support temperature be described target coal seam temperature, add suitable Nutrient medium and trace element, initial salinity and pH value all with adopt
Go out water sample consistent, obtain microbiologic population by cultivation repeatedly and switching；
III. the hydrolysed ferment antibacterial in described step II microbiologic population, hydrogen-producing acetogens, sulfate reduction are refined
Bacterium and methanogen, carry out separating and purification respectively, and after each purifying agaric 3 generation, picking different strains combines, and implants to culture medium
In, the height of contrast methane production, with 30 days as cycle；
IV. the strain combination quantity of strain the most favourable in described step III or different compatibility is expanded, at fermentation tank
Strain or strain that the middle accumulative methane production described step III found is the highest carry out fermentable, expand strain number,
Strengthen bacterial activity.
It is also preferred that the left the strain combinations in described step III includes: a. hydrolysed ferment antibacterial, hydrogen-producing acetogens, b. sulphuric acid
Salt reducing bacteria, c. methanogen, d. hydrolysed ferment antibacterial, hydrogen-producing acetogens+sulfate reducting bacteria, e. hydrolysed ferment antibacterial,
Hydrogen-producing acetogens+methanogen, f. sulfate reducting bacteria+methanogen, g. hydrolysed ferment antibacterial, hydrogen-producing acetogens+sulfur
Hydrochlorate reducing bacteria+methanogen；
It is also preferred that the left described step (2) comprises the following steps:
I. at laboratory, utilize target coal sample to prepare described experimental sample, carry out described ultrasonic wave-coupled supercritical CO2Place
Reason, enters described laboratory operation management system, determines described ultrasonic wave-coupled supercritical CO2Process the optimal real of experiment coal seam
Test condition, including temperature, pressure, time；
II. according to the temperature determined in described step I, pressure, time, target coal seam is carried out described ultrasonic wave-coupled and surpasses
Critical CO2Process, extract, dissolve partial organic substances in coal, improve coal seam constitution pores and crack, promote methane desorbing；
III. the methane of output is collected in production wellhead, to reduce coal seam pressure.
It is also preferred that the left described step (3) comprises the following steps:
I. the gas componant of detection production wellhead and concentration change, use coal bed gas well routine bore mode by described step
(1) fermentation liquid obtained in is slowly injected in the target coal seam that described step (2) processes, and sealing of hole in time；
II. when methane concentration meets and utilizes condition, by the way of drainage and step-down mining, the methane of production is opened
Adopt and utilize.
It is also preferred that the left described step (1) uses methanogen group structure described in clone library technical Analysis, by qPCR skill
Art quantitative analysis strain.
It is also preferred that the left each combination in step III in described step (1) all adds anaerobic fermentation bacterium, strengthen the fall of coal
Solution degree, and determine that bacterium colony best to target coal seam aerogenesis combines by the collocation of various combination, avoid ecotone simultaneously
Inhibitory action.
It is also preferred that the left the collection of described pressure is carried out by described data collection module by pressure transducer, described pressure passes
Sensor quantity is more than or equal to three；The pressure transducer each time that described data collection module is gathered by described data processing module
Data screen, then the meansigma methods of the pressure data obtained by described pressure transducer is P, the most described average value P
Computing formula is:
In formula, WiComputing formula be:
In formula, P is the meansigma methods of pressure data, and i, j are respectively the sequence number of described pressure transducer, Pi、PjIt is respectively the
The pressure that i, j pressure transducer obtains, n is described pressure transducer quantity, WiFor pressure value PiCorresponding coefficient value, NiFor
Pressure value PiCorresponding intermediate value.
It is also preferred that the left described ultrasonic wave-coupled supercritical CO2The treatment conditions processed include that ultrasonic Treatment condition is faced with super
Boundary CO2Treatment conditions, described ultrasonic Treatment condition is: pressure 0.5-2.5Mpa, processes time 10-60min, described supercritical
CO2Treatment conditions are: temperature is consistent with described target coal seam, pressure 7.4-9.6MPa, process time 6h-54h.
Compared with prior art, the invention has the beneficial effects as follows: (1), by accurately controlling the addition of trace element, carries
The output tolerance of high methanogen；(2) utilizing origin bacteria microorganism degraded coal, the microorganism being effectively improved in subterranean coal is dropped
Solve and angry ability, improve hole, coal seam and ooze structure, promote the production of coal bed gas；(3) ultrasonic wave-coupled supercritical CO2Process, change
Kind coal bed texture, strengthens the methanogenic efficiency of biodegradation coal, promotes the desorbing of methane gas in coal body, accelerates coal bed gas aerogenesis speed
Rate, with simple supercritical CO2Process is compared, and is effectively shortened the process time, increases factor of created gase, reduces energy consumption；(4) utilize in fact
Test room operation management system to analyze, calculate speed soon, be effectively increased work efficiency.
Detailed description of the invention
To the present invention, above-mentioned and other technical characteristic and advantage are described in more detail below.
Embodiment one:
A kind of method utilizing origin bacterium to improve methane output that the present invention provides, step is as follows:
(1) target coal seam gas field is certain region, Junggar Basin, Xinjiang, china south, gathers the reservoir coal sample of Occurrence of Coalbed Methane,
Being sampled at coal bed gas well head, sample bottle sterilizing in advance and full nitrogen, water sample takes and completely remains without air to bottle, 24 hours
Within send in anaerobic culture box in the way of shading；From described coal sample and water sample, refine colony structure, pass through clone library
The described biological community structure of described coal sample and water sample is analyzed by technology, by qPCR technology quantitative analysis strain.
The target coal seam of cbm development is the coal seam of system Xishanyao group in Jurassic system, and reservoir pressure is 3.8MPa, reservoir
Temperature is 32 DEG C, and the pH value of output water is 7.2.The kind of antibacterial is mainly Firmicutes (Firmicutes) and actinomycetes door
(Actinobacteria)；The ancient mushroom group of methanogen is Methanosarcina (Methanosarcina) and methane leaf bacterium
Belong to (Methanolobus).
(2) using the coal petrography of target coal seam as carbon source, microorganisms flora from output water sample and coal sample, including hydrolysis
Zymogenous bacteria, hydrogen-producing acetogens, sulfate reducting bacteria and methanogen.Keeping consistent with the salinity of output water, pH value sets
Being 7.2, cultivation temperature 32 DEG C, salinity is 1500mg/L.Microorganism enrichment and separation is carried out, by flat in anaerobic culture box
Plate segmentation and line separate and separate antibacterial and methanogen and purification, different bacterium colony combinations are trained in the medium
Support.
Refining the antibacterial in microbiologic population, described antibacterial includes hydrolysed ferment antibacterial, hydrogen-producing acetogens, except this it
Outward, possibly together with sulfate reducting bacteria in microbiologic population, by described hydrolysed ferment antibacterial, hydrogen-producing acetogens, sulfate reduction
Bacterium and described methanogen, carry out separating and purification respectively, and after each purifying agaric 3 generation, picking different strains combines, described bacterial strain
Combination includes a. hydrolysed ferment antibacterial and hydrogen-producing acetogens, b. sulfate reducting bacteria, c. methanogen, d. hydrolysed ferment antibacterial
With hydrogen-producing acetogens+sulfate reducting bacteria, e. hydrolysed ferment antibacterial and hydrogen-producing acetogens+methanogen, f. sulfate also
Former bacterium+methanogen, g. hydrolysed ferment antibacterial and hydrogen-producing acetogens+sulfate reducting bacteria+methanogen, implant to culture medium
In, with 30 days as cycle, it is enriched with and separates.
The composition of described culture medium is for be made up of basal medium and trace element solution, and ratio is 100:1, wherein:
1000mL basal medium includes: K2HPO40.4g, KH2PO40.4g, MgCl2 2.0g,NH4Cl 1.0g,KCl
0.2g, NaCl 0.2g, yeast immerses liquid 1.0g, sodium acetate 2.0g, "diazoresorcinol" 0.01g；
1000mL trace element includes: NaCl 1.0g, FeSO4·7H2O 0.1g,MgSO4·7H2O 3.0g,CuSO4·
5H2O 0.01g,KAl(SO4)2 0.01g,H3BO3 0.01g,CuSO4·5H2O 0.01g,ZnSO4·7H2O0.1g,CoCl2
0.1g。
(3) the coal petrography accumulative methane production of output in 30 days with the described target coal seam of unit mass refers to for evaluating
Mark, contrasts described hydrolysed ferment antibacterial, hydrogen-producing acetogens and methanogen volume variance under different ratio, selects and produce
The proportion compatibility that methane content is the highest.Contrast described hydrolysed ferment antibacterial, hydrogen-producing acetogens and methanogen under different ratio
Volume variance, selecting the highest proportion compatibility of methane production is 4:1.
(4) enter laboratory operation management system, laboratory operation management system include server, data collection module,
Data processing module and control module；
Described data collection module, described data processing module and described control module are respectively at described server communication even
Connect；
Described data collection module is connected with described data processing module communication, is used for gathering ultrasonic wave-coupled supercritical
CO2Process the experiment parameter of coal seam sample, be biodegradable output including temperature, pressure, time and the unit mass coal processed
Methane content；
Described data processing module is connected with described control module communication, is used for analyzing described data collection module and collects
Information, calculate optimal experiment parameter；
Described control module, is used for arranging ultrasonic wave-coupled supercritical CO2Process the experiment parameter of coal seam sample and show
Show the analysis result of described data processing module.
(5) at laboratory, utilize target coal sample to prepare standard specimen, carry out ultrasonic wave-coupled supercritical CO2Process, pass through
Described laboratory operation management system, arranging pressure is 0.5-2.5Mpa, and experiment coal seam is carried out ultrasonic Treatment, supersonic frequency
For 40-80kHz, the process time is 10-60min.
Then, under the conditions of temperature is 32 DEG C, described temperature keeps consistent with described target coal seam, and pressure adjusting range is
7.4-9.6MPa, carries out supercritical CO to experiment coal seam2Processing, the process time is 6h-54h.
At described ultrasonic Treatment process or described supercritical CO2In processing procedure, pressure can be along with experiment coal seam first
The change of the output value of alkane and change, need record the force value in described experiment coal seam in time and take measures on customs clearance rapidly,
Pressure oscillation in experimentation can be reduced so that described ultrasonic Treatment process or described supercritical CO2Processing procedure keeps
At pressure constant state.The collection of described pressure is carried out by described data collection module by pressure transducer, to described experiment coal seam
Arrange at least 3 pressure transducers pressure signal is acquired, and then obtain average pressure.Do so not only makes collection
Data are more comprehensive, and it can be avoided that the interference of uncertain factor in environment.
Described data processing module needs the data recording pressure transducer each time to screen, by described pressure
The meansigma methods of the pressure data that sensor is obtained is P, and the computing formula of the most described average value P is:
In formula, WiComputing formula be:
In formula, P is the meansigma methods of pressure data, and i, j are respectively the sequence number of described pressure transducer, Pi、PjIt is respectively the
The pressure that i, j pressure transducer obtains, n is described pressure transducer quantity, WiFor pressure value PiCorresponding coefficient value, NiFor
Pressure value PiCorresponding intermediate value.
During the calculating of above-mentioned pressure mean values, first obtain the absolute value of a certain force value and each pressure value difference
Sum, sues for peace to this value the most again, the most again divided by the absolute value sum of described a certain force value Yu each pressure value difference, finally
Intermediate value N is obtained again divided by described pressure transducer quantityi；The described intermediate value that different pressure is corresponding is different, corresponding
Described intermediate value be 0 less than 1 by functional operation, be 1 more than 1, so described intermediate value transported by function
The coefficient value of described force value, i.e. W is obtained after calculationi, as the coefficient of corresponding force value, described intermediate value fluctuation range is relatively
Little coefficient is 1, and coefficient value described in remainder data is 0, this eliminates the bigger force value of fluctuation, thus obtain more accurate
Actual mean pressure value.Finally, it is thus achieved that after this pressure mean values, compare in the force value with initially setting up, if
Fluctuation is little, then without being adjusted, but if undulating value is beyond certain scope, this scope can according to circumstances be adjusted
Whole, then need to carry out pressurizeing or blood pressure lowering process so that force value fluctuates in the range of setup pressure value.
The intermediate value of described force value can be directly obtained by above-mentioned formula, and be calculated described pressure by function
The coefficient value of force value, the coefficient value obtained by the force value that described fluctuation range is bigger is calculated by function is 0, so calculates letter
Folk prescription just and can get rid of the numerical value that fluctuation range is bigger faster so that obtained pressure mean values is closer to actual pressure
Value, improves the accuracy of data result, eliminates what measurement was caused by surrounding and foreign body etc. in data acquisition
Impact, reduces the error incidence rate of DATA REASONING.Compared with the prior art, this data processing method is carried out by the formula quantified
Calculate, get rid of abnormal data process simple, perform motivated, calculate rapidly and make obtained pressure mean values data more
Add accurately.
The mechanism of action of described ultrasonic Treatment is mainly mechanical vibration, cavitation and heat effect.The machinery of ultrasound wave
Vibration mechanism is different from wherein fluid density based on coal petrography skeleton, and the acceleration of generation is different, and mechanical vibration make coal petrography skeleton
And granule produces vibration, biphase material interface generation relative motion, make the methane gas adhesive force on coal petrography surface weaken, promote
The desorption of methane gas and desorbing.Meanwhile, mechanical vibration have and reduce the impact of boundary-layer in hole or pore constriction, make coal petrography hole
Gap effective radius increases, and flowing velocity increases.
The cavitation of described ultrasound wave, during due to ultrasound wave by liquid, can make the microbubble in medium resonate,
Swell rapidly, then bury in oblivion, improve the permeance property of coal bed gas well near wellbore zone.
The heat effect of described ultrasound wave is that the energy propagated in coal body due to ultrasound wave is by coal rock medium, coal seam water and first
Alkane absorbs and is converted into heat energy, makes coal body particle temperature raise, increases the kinetic energy of molecule, reduces methane gas and divide with coal surface
Absorption affinity between son, promotes the desorbing of methane gas in coal body.
And described supercritical CO2Processing is then to utilize supercritical CO2The diffusion coefficient of superelevation and solvability, improve coal seam
Structure, strengthens the methanogenic efficiency of biodegradation coal.Described ultrasonic wave-coupled supercritical CO2Process, by ultrasound wave and supercritical
CO2Extraction phase combines, and improves supercritical CO further2Diffusion coefficient and solvability, under identical extraction conditions, ultrasound wave
Auxiliary supercritical CO2Extraction can be by original supercritical CO2Extraction efficiency improves 10～23%, significantly improves supercritical CO2Extraction
Efficiency.
By described laboratory operation management system, the data collected are analyzed place by described data processing module
Reason, and obtains optimal parameter, and carries out data by described control module and show.
(6) described ultrasonic wave-coupled supercritical CO is carried out in target coal seam2Process, extract, dissolve partial organic substances in coal,
Improve coal seam constitution pores and crack, promote methane desorbing；
(7) collect due to described ultrasonic wave-coupled supercritical CO in target area production wellhead2Process and the coal of displacement generation
Layer gas (methane), analyzes gas componant；
(8) the aerogenesis advantageous combination step (3) screened carries out large-scale enrichment culture in fermentation tank,
Described target area uses coal bed gas well routine bore mode to be slowly injected into the fermentation liquid obtained in described fermentation tank through described
Ultrasonic wave-coupled supercritical CO2In the target coal seam processed, and sealing of hole in time.
(9) persistently gas componant and the methane concentration of detection aerogenesis mouth change, and present at methane concentration and continue to increase trend
Afterwards, closing on well and carrying out the drainage and step-down mining of coal bed gas well, when methane concentration presents downward trend, adding institute in good time
State fermentation liquid.
Embodiment 2
On the basis of above-mentioned embodiment, the optimal parameter that described step (5) obtains is: described ultrasonic Treatment is optimal
Pressure value is 0.8Mpa, and supersonic frequency is 80kHz, and the process time is 20min, described supercritical CO2Process optimal parameter pressure
Power is 8.2MPa, and the process time is 30h, under this condition, experiment coal seam is carried out described ultrasonic wave-coupled supercritical CO2Process
The methane content that rear unit mass coal is biodegradable output is most.
In described step (9), gas componant and the methane concentration of aerogenesis mouth change by Methane determination instrument and gas chromatograph
Measure.
Embodiment 3
On the basis of above-mentioned embodiment, in described step (2), each strain combinations all adds anaerobic fermentation bacterium, strengthens coal
Palliating degradation degree, and determine that bacterium colony best to target coal seam aerogenesis combines by the collocation of various combination, avoid group simultaneously
Inhibitory action between falling.
Embodiment 4
On the basis of above-mentioned embodiment, described in embodiment 1 step (2), the addition of trace element is to methane production shadow
Ring bigger.In the methane phase stage of anaerobic digestion, methanogen is the most sensitive to the shortage of described trace element.Described trace unit
The shortage of element can cause volatile fatty acid in water outlet higher, and under gas yield, degradation bad phenomenon occurs.It addition, described trace
The addition of element has antagonism to toxicant, thus alleviates the toxicant restriction effect to methanogen.Thus institute
Stating the addition of trace element can make the dominant bacteria of methanogen in described culture medium occupy certain advantage, thus increases described
Methane production.In order to ensure that described trace element is made full use of by described methanogen, described trace element directly adds
Enter in described culture medium.Addition X of trace element in described culture mediumiAnd exist with ShiShimonoseki between described methane production V
System:
In formula,
N: trace element in total n in culture medium；
Xi: i-th kind of micronutrient levels (g) in culture medium；
V: methane production (ml).
Described trace element is meeting accurate weighing before adding culture medium, thus obtains Xi.Then, calculate i.e. according to formula
Under available different proportion, described product adds the numerical value of methane content.And traditional method, described methane production generally uses fluid-discharge therapy to enter
Row is measured, and this method of testing needs to prepare in advance correlation meter, and during measuring, described measurement apparatus is with described
Need strict being tightly connected between culture medium, need to consume certain manpower and materials, and test process is stressed, sealing etc.
Extraneous factor impact is relatively big, and measuring result error is bigger.And by this quantitative equation, in the addition determining described trace element
Amount, it is possible to obtain described methane production, save manpower and materials, and result of calculation will not be affected by extraneous factor, it is thus achieved that
Described methane production is quick and accurate.And, by this formula, it is possible to achieve the accuracy controlling of described medium component.
Although the present invention being described in detail with reference to previous embodiment, for a person skilled in the art,
Technical scheme described in foregoing embodiments still can be modified by it, or carries out wherein portion of techniques feature
With replacing, all within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made, should be included in this
Within the protection domain of invention.
Claims (10)
1. one kind utilizes the method that origin bacterium improves methane output, it is characterised in that comprise the following steps:
(1) enrichment of methanogen group and cultivation: from target area samples of coal pulled and water sample, carry out the richness of methanogen group
Collection is cultivated and fermentation, obtains fermentation liquid；
(2) ultrasonic wave-coupled supercritical CO2Process: utilize laboratory operation management system, surpass at lab analysis ultrasonic wave-coupled
Critical CO2Process the optimum condition of coal seam sample, and at optimum conditions target coal seam is processed；
(3) microbial degradation coal: the fermentation liquid described step (1) obtained injects described target coal seam, and persistently well is injected in detection
Gas component and methane concentration change, and utilize the mode of drainage and step-down mining to collect methane.
A kind of method utilizing origin bacterium to improve methane output, it is characterised in that described step
(2) the laboratory operation management system in includes server, data collection module, data processing module and control module；
Described data collection module, described data processing module and described control module are connected with described server communication respectively；
Described data collection module is connected with described data processing module communication, is used for gathering described ultrasonic wave-coupled supercritical CO2
Processing the experiment parameter of coal seam sample, described experiment parameter includes that temperature, pressure, time and unit mass coal are biodegradable product
The methane content gone out；
Described data processing module is connected with described control module communication, for analyzing the letter that described data collection module collects
Breath, calculates optimal experiment parameter, i.e. unit mass coal and is biodegradable the most temperature in moment of the methane content of output, pressure
Power and time；
Described control module, is used for arranging ultrasonic wave-coupled supercritical CO2The experiment parameter and the display that process coal seam sample are described
The analysis result of data processing module.
A kind of method utilizing origin bacterium to improve methane output, it is characterised in that described step
Suddenly (1) comprises the following steps:
I. being sampled at coal bed gas well head, sample bottle sterilizing in advance and full nitrogen, water sample takes full to residual without air in bottle
Stay, send in anaerobic culture box in the way of shading within 24 hours；
II. with target coal seam as carbon source, from described step I output water sample, cultivate microorganism species therein, enrichment culture
Temperature is described target coal seam temperature, adds suitable Nutrient medium and trace element, initial salinity and pH value all and recovered water
Sample is consistent, obtains microbiologic population by cultivation repeatedly and switching；
III. refine hydrolysed ferment antibacterial, hydrogen-producing acetogens, sulfate reducting bacteria and the product in described step II microbiologic population
Methane backeria, carries out separating and purification respectively, and after each purifying agaric 3 generation, picking different strains combines, and implants to culture medium, right
The ratio height of methane production, with 30 days as cycle；
IV. the strain combination quantity of strain the most favourable in described step III or different compatibility is expanded, will in fermentation tank
The highest strain of accumulative methane production or strain that described step III finds carry out fermentable, expand strain number, strengthen
Bacterial activity.
A kind of method utilizing origin bacterium to improve methane output, it is characterised in that described step
Strain combinations in III includes: a. hydrolysed ferment antibacterial, hydrogen-producing acetogens, b. sulfate reducting bacteria, c. methanogen, d. water
Hydrolysis and fermentation antibacterial, hydrogen-producing acetogens+sulfate reducting bacteria, e. hydrolysed ferment antibacterial, hydrogen-producing acetogens+methanogen, f.
Sulfate reducting bacteria+methanogen, g. hydrolysed ferment antibacterial, hydrogen-producing acetogens+sulfate reducting bacteria+methanogen.
A kind of method utilizing origin bacterium to improve methane output, it is characterised in that described step
(2) comprise the following steps:
I. at laboratory, utilize target coal sample to prepare described experimental sample, carry out described ultrasonic wave-coupled supercritical CO2Process, enter
Enter described laboratory operation management system, determine described ultrasonic wave-coupled supercritical CO2Process experiment coal seam most preferably tests bar
Part, including temperature, pressure, time；
II. according to the temperature determined in described step I, pressure, time, target coal seam is carried out that described ultrasonic wave-coupled is super to be faced
Boundary CO2Process, extract, dissolve partial organic substances in coal, improve coal seam constitution pores and crack, promote methane desorbing；
III. collect the methane of output in production wellhead, to reduce coal seam pressure.
A kind of method utilizing origin bacterium to improve methane output, it is characterised in that described step
(3) comprise the following steps:
I. the gas componant of detection production wellhead and concentration change, use coal bed gas well routine bore mode by described step (1)
The fermentation liquid of middle acquisition is slowly injected in the target coal seam that described step (2) processes, and sealing of hole in time；
II. when methane concentration meets and utilizes condition, carry out exploiting also by the methane of production by the way of drainage and step-down mining
Utilize.
A kind of method utilizing origin bacterium to improve methane output, it is characterised in that described step
(1) methanogen group structure described in clone library technical Analysis is used, by qPCR technology quantitative analysis strain.
A kind of method utilizing origin bacterium to improve methane output, it is characterised in that described step
(1) each combination in the step III in all adds anaerobic fermentation bacterium, strengthens the palliating degradation degree of coal, and taking by various combination
Join and determine that bacterium colony best to target coal seam aerogenesis combines, avoid the inhibitory action of ecotone simultaneously.
A kind of method utilizing origin bacterium to improve methane output, it is characterised in that described data
The collection of described pressure is carried out by collection module by pressure transducer, and described pressure transducer quantity is more than or equal to three；Institute
The data stating the pressure transducer each time that described data collection module is gathered by data processing module are screened, then pass through institute
The meansigma methods stating the pressure data that pressure transducer obtains is P, and the computing formula of the most described pressure mean values P is:
In formula, WiComputing formula be:
In formula, P is the meansigma methods of pressure data, and i, j are respectively the sequence number of described pressure transducer, Pi、PjRespectively i-th, j
The pressure that individual pressure transducer obtains, n is described pressure transducer quantity, WiFor pressure value PiCorresponding coefficient value, NiFor pressure
Value PiCorresponding intermediate value.
A kind of method utilizing origin bacterium to improve methane output, it is characterised in that described super
Sound wave coupling supercritical CO2The treatment conditions processed include ultrasonic Treatment condition and supercritical CO2Treatment conditions, described ultrasonic
Ripple treatment conditions are: pressure 0.5-2.5Mpa, process time 10-60min, described supercritical CO2Treatment conditions are: temperature and institute
State target coal seam consistent, pressure 7.4-9.6MPa, process time 6h-54h.
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| CN107387043A (en) * | 2017-08-14 | 2017-11-24 | 西南石油大学 | A kind of method that spontaneous multicomponent gas displacement in coal seam improves coal bed gas recovery ratio |
| CN107387044A (en) * | 2017-08-22 | 2017-11-24 | 太原理工大学 | A kind of method that biological methane output is improved using coal seam origin fungi |
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| CN111855377A (en) * | 2020-07-28 | 2020-10-30 | 太原理工大学 | Supercritical CO2Test device and method for producing methane by extracting coal coupling biological reaction |
| CN112528927A (en) * | 2020-12-22 | 2021-03-19 | 北京百度网讯科技有限公司 | Confidence determination method based on trajectory analysis, roadside equipment and cloud control platform |
| CN112528927B (en) * | 2020-12-22 | 2024-05-10 | 阿波罗智联(北京)科技有限公司 | Confidence determining method based on track analysis, road side equipment and cloud control platform |
| CN112922599B (en) * | 2021-04-08 | 2022-02-08 | 中国矿业大学 | Biological-high temperature gasification combined mining method for hydrogen production from coal |
| CN112922599A (en) * | 2021-04-08 | 2021-06-08 | 中国矿业大学 | Biological-high temperature gasification combined mining method for hydrogen production from coal |
| CN113738322B (en) * | 2021-09-01 | 2022-04-26 | 中国矿业大学 | Method for changing coal permeability by using hydrogen-producing acetogenic bacteria |
| CN113738322A (en) * | 2021-09-01 | 2021-12-03 | 中国矿业大学 | Method for changing coal permeability by using hydrogen-producing acetogenic bacteria |
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