CN106285581B - A method of improving methane output using origin bacterium - Google Patents
A method of improving methane output using origin bacterium Download PDFInfo
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- CN106285581B CN106285581B CN201610710769.1A CN201610710769A CN106285581B CN 106285581 B CN106285581 B CN 106285581B CN 201610710769 A CN201610710769 A CN 201610710769A CN 106285581 B CN106285581 B CN 106285581B
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- E—FIXED CONSTRUCTIONS
- E21—EARTH DRILLING; MINING
- E21B—EARTH DRILLING, e.g. DEEP DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/006—Production of coal-bed methane
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH DRILLING; MINING
- E21B—EARTH DRILLING, e.g. DEEP DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH DRILLING; MINING
- E21B—EARTH DRILLING, e.g. DEEP DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
- E21B43/164—Injecting CO2 or carbonated water
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Abstract
The present invention provides a kind of method improving methane output using origin bacterium, includes the enrichment and culture of (1) methanogen group：From target area samples of coal pulled and water sample, enrichment culture and the fermentation of methanogen group are carried out, zymotic fluid is obtained；(2) ultrasonic wave-coupled supercritical CO2Processing：Using laboratory operation management system, in lab analysis ultrasonic wave-coupled supercritical CO2The optimum condition of coal seam sample is handled, and target coal seam is handled at optimum conditions；(3) microbial degradation coal：The zymotic fluid that the step (1) is obtained injects the target coal seam, persistently detects injection well gas component and methane concentration variation, and collect methane in the way of drainage and step-down mining.The present invention utilizes ultrasonic wave-coupled supercritical CO2Processing effectively shortens processing time, accelerates coal bed gas gas production rate, while effectively increasing working efficiency using laboratory operation management system.
Description
Technical field
It is specially a kind of to improve methane output using origin bacterium the present invention relates to raising methane output technical field
Method.
Background technology
Coal bed gas has carried out exploration and development in high, medium and low rank coal basin, but low-order coal air content is low, the infiltration of high-order coal
The low present situation of rate seriously constrains the development of coal gas industry.Increased production using microorganism, i.e., injects the micro- life of methane phase into coal seam
Object group, the organic matter in coal of degrading, increases the content of methane, and improve the hole knot in coal seam by consuming the matrix in coal
Structure improves permeability, can greatly improve the yield of coal bed gas.
The patent of Patent No. 201410363562.2 discloses a kind of method improving coal bed gas recovery ratio, specially sharp
With coal seam in-situ microorganism species and supercritical CO2Coal seam is pre-processed, improves coal bed gas recovery ratio, but supercritical CO2Processing
Time is longer, and long-term work energy consumption is higher and needs manually to compare, and determines that optimum experimental condition, working efficiency are low.
In view of drawbacks described above, creator of the present invention obtains the present invention finally by prolonged research and practice.
Invention content
To solve above-mentioned technological deficiency, the technical solution adopted by the present invention is, provides a kind of utilization origin bacterium raising coal
The method of layer gas yield, includes the following steps:
(1) enrichment and culture of methanogen group：From target area samples of coal pulled and water sample, methanogen group is carried out
Enrichment culture and fermentation, obtain zymotic fluid；
(2) ultrasonic wave-coupled supercritical CO2Processing：Using laboratory operation management system, in lab analysis ultrasonic wave
Couple supercritical CO2The optimum condition of coal seam sample is handled, and target coal seam is handled at optimum conditions；
(3) microbial degradation coal：The zymotic fluid that the step (1) is obtained injects the target coal seam, lasting detection note
Enter well head gas component and methane concentration variation, and methane is collected in the way of drainage and step-down mining.
Preferably, the laboratory operation management system in the step (2) includes server, data collection module, data
Processing module and control module；
The data collection module, the data processing module and the control module are communicated with the server respectively to be connected
It connects；
The data collection module is connected with data processing module communication, and for acquiring, the ultrasonic wave-coupled is super to be faced
Boundary CO2The experiment parameter of coal seam sample is handled, the experiment parameter includes that temperature, pressure, time and unit mass coal are biological
The methane content for output of degrading；
The data processing module is connected with control module communication, is collected for analyzing the data collection module
Information, calculate best experiment parameter, i.e., unit mass coal be biodegradable output methane content it is most at the time of temperature
Degree, pressure and time；
The control module, for ultrasonic wave-coupled supercritical CO to be arranged2It handles the experiment parameter of coal seam sample and shows
Show the analysis result of the data processing module.
Preferably, the step (1) includes the following steps：
I, is sampled at coal bed gas well head, and sample bottle sterilizes and is full of nitrogen in advance, and water sample, which takes, expires into bottle without sky
Gas remains, and is sent into anaerobic culture box in a manner of shading within 24 hours；
II, cultivates microorganism species therein, enrichment training using target coal seam as carbon source from the step I output water sample
Foster temperature is the target coal seam temperature, adds suitable Nutrient medium and trace element, initial salinity and pH value with adopt
Go out that water sample is consistent, microbiologic population is obtained by culture repeatedly and switching；
III. hydrolysed ferment bacterium in the step II microbiologic population, hydrogen-producing acetogens, sulfate reduction are refined
Bacterium and methanogen, are detached and are purified respectively, after 3 generation of each purifying agaric, the combination of picking different strains, and implantation to culture medium
In, the height of methane production is compared, with 30 days for the period；
IV, expands the strain combination quantity of most advantageous strain in the step III or different compatibilities, in fermentation tank
The middle highest strain of accumulative methane production for finding the step III or strain carry out microbial fermentation, expand strain number,
Enhance bacterial activity.
Preferably, the bacterial strain combination in the step III includes：A. hydrolysed ferment bacterium, hydrogen-producing acetogens, b. sulfuric acid
Salt reducing bacteria, c. methanogens, d. hydrolysed ferments bacterium, hydrogen-producing acetogens+sulfate reducing bacteria, e. hydrolysed ferments bacterium,
Hydrogen-producing acetogens+methanogen, f. sulfate reducing bacterias+methanogen, g. hydrolysed ferments bacterium, hydrogen-producing acetogens+sulphur
Hydrochlorate reducing bacteria+methanogen；
Preferably, the step (2) includes the following steps：
I, prepares the experimental sample in laboratory, using target coal sample, carries out the ultrasonic wave-coupled supercritical CO2Place
Reason, into the laboratory operation management system, determines the ultrasonic wave-coupled supercritical CO2The best reality in processing experiment coal seam
Test condition, including temperature, pressure, time；
II, carries out the ultrasonic wave-coupled according to the temperature, pressure, time determined in the step I, to target coal seam and surpasses
Critical CO2Partial organic substances in coal are dissolved in processing, extraction, improve coal seam constitution pores and crack, promote methane desorption；
III. the methane that output is collected in production wellhead, to reduce coal seam pressure.
Preferably, the step (3) includes the following steps：
I, detects gas componant and the concentration variation of production wellhead, using coal bed gas well routine bore mode by the step
(1) zymotic fluid obtained in is slowly injected into the target coal seam handled through the step (2), and timely sealing of hole；
II, is opened the methane of production by way of drainage and step-down mining when methane concentration meets using condition
It adopts and utilizes.
Preferably, the step (1) analyzes methanogen group structure using clone library technology, pass through qPCR skills
Art quantitative analysis strain.
Preferably, anaerobic fermentation bacterium is added in each combination in step III in the step (1), enhance the drop of coal
Solution degree, and determined by the collocation of various combination and the best bacterium colony of target coal seam aerogenesis is combined, while avoiding ecotone
Inhibiting effect.
Preferably, the data collection module carries out the collection of the pressure by pressure sensor, the pressure passes
Sensor quantity is more than or equal to three；The pressure sensor each time that the data processing module acquires the data collection module
Data screened, then the average value of the pressure data obtained by the pressure sensor is P, then the average value P
Calculation formula is：
In formula, WiCalculation formula be：
In formula, P is the average value of pressure data, and i, j are respectively the serial number of the pressure sensor, Pi、PjRespectively
I, the pressure that j pressure sensor obtains, n are the pressure sensor quantity, WiFor pressure value PiCorresponding coefficient value, NiFor
Pressure value PiCorresponding intermediate value.
Preferably, the ultrasonic wave-coupled supercritical CO2The treatment conditions of processing include that ultrasonication condition is faced with super
Boundary CO2Treatment conditions, the ultrasonication condition are：Pressure 0.5-2.5Mpa, processing time 10-60min, it is described overcritical
CO2Treatment conditions are：Temperature is consistent with the target coal seam, pressure 7.4-9.6MPa, processing time 6h-54h.
Compared with prior art, the beneficial effects of the invention are as follows：(1) it by accurately controlling the additive amount of trace element, carries
The output tolerance of high methanogen；(2) origin bacteria microorganism degradation coal is utilized, the microorganism drop in subterranean coal can be effectively improved
Solution and angry ability, improve coal seam hole and ooze structure, promote the production of coal bed gas；(3) ultrasonic wave-coupled supercritical CO2Processing, changes
Kind coal bed texture enhances the efficiency of biodegradable coal methane phase, promotes the desorption of methane gas in coal body, accelerates coal bed gas aerogenesis speed
Rate, with simple supercritical CO2Processing is compared, and processing time is effectively shortened, and increases factor of created gase, reduces energy consumption；(4) using real
It is fast to test the analysis of room operation management system, calculating speed, effectively increases working efficiency.
Specific implementation mode
The forgoing and additional technical features and advantages are described in more detail below.
Embodiment one：
A kind of method improving methane output using origin bacterium provided by the invention, steps are as follows：
(1) target coal seam gas field is certain southern region of Junggar Basin, Xinjiang, china, acquires the reservoir coal sample of Occurrence of Coalbed Methane,
It is sampled at coal bed gas well head, sample bottle sterilizes and is full of nitrogen in advance, and water sample takes to expire and be remained into bottle without air, 24 hours
Within be sent into anaerobic culture box in a manner of shading；Colony structure is refined from the coal sample and water sample, passes through clone library
Technology analyzes the biological community structure of the coal sample and water sample, passes through qPCR technology quantitative analysis strains.
The target coal seam of cbm development is the coal seam of system Xishanyao group in Jurassic system, reservoir pressure 3.8MPa, reservoir
Temperature is 32 DEG C, and the pH value of output water is 7.2.The type of bacterium is mainly Firmicutes (Firmicutes) and actinomyces door
(Actinobacteria)；The ancient mushroom group of methanogen is Methanosarcina (Methanosarcina) and methane leaf bacterium
Belong to (Methanolobus).
(2) using the coal petrography of target coal seam as carbon source, the microorganisms flora from output water sample and coal sample, including hydrolysis
Zymogenous bacteria, hydrogen-producing acetogens, sulfate reducing bacteria and methanogen.It is consistent with the salinity of output water, pH value is set
It is 7.2,32 DEG C of cultivation temperature, salinity 1500mg/L.Microorganism enrichment and separation is carried out in anaerobic culture box, by flat
Plate is divided and scribing line separation is detached and purified to bacterium and methanogen, and different bacterium colony combinations is trained in the medium
It supports.
The bacterium in microbiologic population is refined, the bacterium includes hydrolysed ferment bacterium, hydrogen-producing acetogens, except this it
Outside, also contain sulfate reducing bacteria in microbiologic population, by the hydrolysed ferment bacterium, hydrogen-producing acetogens, sulfate reduction
Bacterium and the methanogen, are detached and are purified respectively, after 3 generation of each purifying agaric, the combination of picking different strains, and the bacterial strain
Combination includes a. hydrolysed ferments bacterium and hydrogen-producing acetogens, b. sulfate reducing bacterias, c. methanogens, d. hydrolysed ferment bacteriums
Also with hydrogen-producing acetogens+sulfate reducing bacteria, e. hydrolysed ferments bacterium and hydrogen-producing acetogens+methanogen, f. sulfate
Opportunistic pathogen+methanogen, g. hydrolysed ferments bacterium and hydrogen-producing acetogens+sulfate reducing bacteria+methanogen, implantation to culture medium
In, with 30 days for the period, carry out enrichment and separation.
The ingredient of the culture medium is to be made of basal medium and trace element solution, ratio 100：1, wherein：
1000mL basal mediums include：K2HPO40.4g, KH2PO40.4g, MgCl2 2.0g,NH4Cl 1.0g,KCl
0.2g, NaCl 0.2g, yeast immerse liquid 1.0g, sodium acetate 2.0g, resazurin 0.01g；
1000mL trace elements include：NaCl 1.0g,FeSO4·7H2O 0.1g,MgSO4·7H2O 3.0g,CuSO4·
5H2O 0.01g,KAl(SO4)2 0.01g,H3BO3 0.01g,CuSO4·5H2O 0.01g,ZnSO4·7H2O0.1g,CoCl2
0.1g。
(3) it is that evaluation refers to the accumulative methane production of the coal petrography of the target coal seam of unit mass output in 30 days
Mark, compares the hydrolysed ferment bacterium, the volume variance of hydrogen-producing acetogens and methanogen under different ratio, selects and produce
The highest proportion compatibility of methane content.The hydrolysed ferment bacterium, hydrogen-producing acetogens and methanogen are compared under different ratio
Volume variance, select the highest proportion compatibility of methane production be 4：1.
(4) enter laboratory operation management system, laboratory operation management system include server, data collection module,
Data processing module and control module；
The data collection module, the data processing module and the control module are communicated respectively at the server to be connected
It connects；
The data collection module is connected with data processing module communication, overcritical for acquiring ultrasonic wave-coupled
CO2The experiment parameter of coal seam sample is handled, including the temperature of processing, pressure, time and unit mass coal are biodegradable output
Methane content；
The data processing module is connected with control module communication, is collected for analyzing the data collection module
Information, calculate best experiment parameter；
The control module, for ultrasonic wave-coupled supercritical CO to be arranged2It handles the experiment parameter of coal seam sample and shows
Show the analysis result of the data processing module.
(5) in laboratory, standard specimen is prepared using target coal sample, carries out ultrasonic wave-coupled supercritical CO2Processing, passes through
The laboratory operation management system, setting pressure are 0.5-2.5Mpa, and ultrasonication, supersonic frequency are carried out to experiment coal seam
For 40-80kHz, processing time 10-60min.
Then under the conditions of temperature is 32 DEG C, the temperature is consistent with the target coal seam, and pressure adjusting range is
7.4-9.6MPa carries out supercritical CO to experiment coal seam2Processing, processing time 6h-54h.
In the ultrasonication process or the supercritical CO2In processing procedure, pressure can be with experiment coal seam first
The variation of the output value of alkane and change, need to measure the pressure value in the experiment coal seam in time and take measures on customs clearance rapidly,
Pressure oscillation in experimentation can be reduced so that the ultrasonication process or the supercritical CO2Processing procedure is kept
In pressure constant state.The data collection module carries out the collection of the pressure by pressure sensor, to the experiment coal seam
Setting at least three pressure sensor is acquired pressure signal, and then finds out average pressure.It does so and not only makes acquisition
Data are more comprehensive, and can avoid the interference of uncertain factor in environment.
The data processing module needs the data measured to pressure sensor each time to screen, and passes through the pressure
The average value for the pressure data that sensor is obtained is P, then the calculation formula of the average value P is：
In formula, WiCalculation formula be：
In formula, P is the average value of pressure data, and i, j are respectively the serial number of the pressure sensor, Pi、PjRespectively
I, the pressure that j pressure sensor obtains, n are the pressure sensor quantity, WiFor pressure value PiCorresponding coefficient value, NiFor
Pressure value PiCorresponding intermediate value.
In the calculating process of above-mentioned pressure mean values, a certain pressure value and each pressure value absolute value of the difference are found out first
The sum of, it then sums again to the value, later again divided by the sum of a certain pressure value and each pressure value absolute value of the difference, finally
Again divided by the pressure sensor quantity obtains intermediate value Ni；The corresponding intermediate value of different pressure is different, corresponding
The intermediate value be less than 1 by functional operation be 0, what it is more than 1 is 1, in this way transports the intermediate value by function
The coefficient value of the pressure value, i.e. W are obtained after calculationi, as the coefficient of corresponding pressure value, the intermediate value fluctuation range compared with
Small coefficient is 1, and coefficient value described in remainder data is 0, more acurrate to obtain this eliminates the pressure value that fluctuation is larger
Actual mean pressure value.Finally, it after obtaining the pressure mean values, is compared with the pressure value initially set up, if
Fluctuation is little, then is not necessarily to be adjusted, but if undulating value exceeds certain range, which can according to circumstances be adjusted
It is whole, then carry out pressurization or decompression processing so that pressure value fluctuates within the scope of setup pressure value.
The intermediate value of the pressure value can be directly obtained by above-mentioned formula, and the pressure is calculated by function
The coefficient value of force value, it is 0 that the larger pressure value of the fluctuation range calculates obtained coefficient value by function, calculates letter in this way
Folk prescription can exclude just and faster the larger numerical value of fluctuation range so that obtained pressure mean values are closer to actual pressure
Value, improves the accuracy of data result, eliminates in data acquisition ambient enviroment and foreign matter etc. caused by measurement
It influences, reduces the error incidence of DATA REASONING.Compared with the prior art, which is carried out by the formula of quantization
It calculates, exclusion abnormal data process is simple, and execution is motivated, calculates rapid and makes obtained pressure mean values data more
It is accurate to add.
The mechanism of action of the ultrasonication is mainly mechanical oscillation, cavitation and fuel factor.The machinery of ultrasonic wave
Vibration mechanism is different from wherein fluid density based on coal petrography skeleton, and the acceleration of generation is different, and mechanical oscillation make coal petrography skeleton
And particle generates vibration, relative motion occurs for two-phase material interface, so that methane gas is weakened in the adhesive force on coal petrography surface, promotes
The desorption of methane gas and desorption.Meanwhile mechanical oscillation have the influence for reducing boundary-layer in hole or pore constriction, make coal petrography hole
Gap effective radius increases, and flowing velocity increases.
The cavitation of the ultrasonic wave when passing through liquid due to ultrasonic wave, can make the microbubble in medium resonate,
It swells rapidly, then buries in oblivion, improve the permeance property of coal bed gas well near wellbore zone.
The fuel factor of the ultrasonic wave is since the energy that ultrasonic wave is propagated in coal body is by coal rock medium, coal seam water and first
Alkane absorbs and is converted into thermal energy, so that coal body particle temperature is increased, increases the kinetic energy of molecule, reduces methane gas and coal surface point
Adsorption capacity between son promotes the desorption of methane gas in coal body.
And the supercritical CO2Processing is then to utilize supercritical CO2The diffusion coefficient and solvability of superelevation improve coal seam
Structure enhances the efficiency of biodegradable coal methane phase.The ultrasonic wave-coupled supercritical CO2Processing, by ultrasonic wave with it is overcritical
CO2Extraction phase combines, and further increases supercritical CO2Diffusion coefficient and solvability, under identical extraction conditions, ultrasonic wave
Assist supercritical CO2Extraction can be by original supercritical CO2Extraction efficiency improves 10~23%, significantly improves supercritical CO2Extraction
Efficiency.
By the laboratory operation management system, the data processing module carries out collected data at analysis
Reason, and optimal parameter is obtained, and displayed data by the control module.
(6) the ultrasonic wave-coupled supercritical CO is carried out in target coal seam2Partial organic substances in coal are dissolved in processing, extraction,
Improve coal seam constitution pores and crack, promotes methane desorption；
(7) it is collected due to the ultrasonic wave-coupled supercritical CO in target area production wellhead2The coal of processing and displacement generation
Layer gas (methane) analyzes gas componant；
(8) the aerogenesis most advantageous combination that step (3) screens is subjected to large-scale enrichment culture in fermentation tank,
The zymotic fluid obtained in the fermentation tank is slowly injected into through described by the target area using coal bed gas well routine bore mode
Ultrasonic wave-coupled supercritical CO2In the target coal seam of processing, and timely sealing of hole.
(9) the persistently gas componant of detection aerogenesis mouth and methane concentration variation continues to increase trend in methane concentration presentation
Later, institute is added in due course when downward trend is presented in methane concentration in the drainage and step-down mining for closing on well progress coal bed gas well
State zymotic fluid.
Embodiment 2
On the basis of the above embodiment, the optimal parameter of step (5) acquisition is：The ultrasonication is best
Pressure value is 0.8Mpa, supersonic frequency 80kHz, processing time 20min, the supercritical CO2Handle optimal parameter pressure
Power is that 8.2MPa, processing time 30h carry out the ultrasonic wave-coupled supercritical CO to experiment coal seam under this condition2Processing
Afterwards unit mass coal be biodegradable output methane content it is most.
The gas componant of aerogenesis mouth and methane concentration variation pass through Methane determination instrument and gas chromatograph in the step (9)
It measures.
Embodiment 3
On the basis of the above embodiment, anaerobic fermentation bacterium is added in each bacterial strain combination in the step (2), enhances coal
Palliating degradation degree, and determined by the collocation of various combination and the best bacterium colony of target coal seam aerogenesis combined, while avoiding group
Inhibiting effect between falling.
Embodiment 4
On the basis of the above embodiment, addition micro- described in 1 step of embodiment (2) is to methane production shadow
Sound is larger.In the methane phase stage of anaerobic digestion, methanogen is very sensitive to the shortage of the trace element.The micro member
The bad phenomenons such as the shortage of element can cause volatile fatty acid in water outlet higher, and gas yield declines occur.In addition, described micro
The addition of element has antagonism to toxicant, to alleviate restriction effect of the toxicant to methanogen.Thus institute
Stating the addition of trace element can make the dominant bacteria of methanogen in the culture medium occupy certain advantage, described in increasing
Methane production.In order to ensure that the methanogen makes full use of the trace element, the trace element directly adds
Enter into the culture medium.Micro- addition X in the culture mediumiExist with ShiShimonoseki between the methane production V
System：
In formula,
n：Trace element in n is shared in culture medium；
Xi：I-th kind of micronutrient levels (g) in culture medium；
V：Methane production (ml).
Trace element meeting accurate weighing before culture medium is added, to obtain Xi.Then, it is according to formula calculating
The numerical value of the production plus methane content under different proportion can be obtained.And conventional method, the methane production generally use fluid-discharge therapy into
Row measures, which needs to prepare correlation meter in advance, and in measurement process, the measuring device with it is described
Stringent sealed connection is needed between culture medium, needs to consume certain manpower and materials, and test process is by pressure, leakproofness etc.
Extraneous factor is affected, and measuring result error is larger.And by the quantitative equation, in the addition that the trace element is determined
Amount, so that it may to obtain the methane production, save manpower and materials, and result of calculation will not be influenced by extraneous factor, obtain
The methane production is quick and accurate.Moreover, by the formula, the accuracy controlling of the medium component may be implemented.
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art,
It still can be with technical scheme described in the above embodiments is modified, or is carried out to which part technical characteristic etc.
With replacing, all within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in this
Within the protection domain of invention.
Claims (9)
1. a kind of method improving methane output using origin bacterium, which is characterized in that include the following steps:
(1) enrichment and culture of methanogen group：From target area samples of coal pulled and water sample, the richness of methanogen group is carried out
Collection culture and fermentation, obtain zymotic fluid；
(2) ultrasonic wave-coupled supercritical CO2Processing：It is super in lab analysis ultrasonic wave-coupled using laboratory operation management system
Critical CO2The optimum condition of coal seam sample is handled, and target coal seam is handled at optimum conditions；
(3) microbial degradation coal：The zymotic fluid that the step (1) is obtained injects the target coal seam, persistently detects injection well
Gas component and methane concentration variation, and collect methane in the way of drainage and step-down mining；
The step (1) includes the following steps：
I, is sampled at coal bed gas well head, and sample bottle sterilizes and is full of nitrogen in advance, and water sample takes full to residual without air in bottle
It stays, is sent into anaerobic culture box in a manner of shading within 24 hours；
II, cultivates microorganism species therein using target coal seam as carbon source from the step I output water sample, enrichment culture
Temperature is the target coal seam temperature, adds suitable Nutrient medium and trace element, and initial salinity and pH value are and recovered water
Sample is consistent, and microbiologic population is obtained by culture repeatedly and switching；
III, refines hydrolysed ferment bacterium, hydrogen-producing acetogens, sulfate reducing bacteria and the production in the step II microbiologic population
Methane backeria is detached and is purified respectively, and after 3 generation of each purifying agaric, the combination of picking different strains is right in implantation to culture medium
Than the height of methane production, with 30 days for the period；
IV, expands the strain combination quantity of most advantageous strain in the step III or different compatibilities, will in fermentation tank
The highest strain of accumulative methane production or strain that the step III is found carry out microbial fermentation, expand strain number, enhancing
Bacterial activity.
2. a kind of method improving methane output using origin bacterium as described in claim 1, which is characterized in that the step
(2) the laboratory operation management system in includes server, data collection module, data processing module and control module；
The data collection module, the data processing module and the control module communicate with the server connect respectively；
The data collection module is connected with data processing module communication, for acquiring the ultrasonic wave-coupled supercritical CO2
The experiment parameter of coal seam sample is handled, the experiment parameter includes that temperature, pressure, time and unit mass coal are biodegradable production
The methane content gone out；
The data processing module is connected with control module communication, for analyzing the collected letter of the data collection module
Breath, calculates best experiment parameter, i.e., unit mass coal be biodegradable output methane content it is most at the time of temperature, pressure
Power and time；
The control module, for ultrasonic wave-coupled supercritical CO to be arranged2It handles described in experiment parameter and the display of coal seam sample
The analysis result of data processing module.
3. a kind of method improving methane output using origin bacterium as described in claim 1, which is characterized in that the step
Bacterial strain in III, which combines, includes：A. hydrolysed ferment bacterium, hydrogen-producing acetogens, b. sulfate reducing bacterias, c. methanogens, d. water
Hydrolysis and fermentation bacterium, hydrogen-producing acetogens+sulfate reducing bacteria, e. hydrolysed ferments bacterium, hydrogen-producing acetogens+methanogen, f.
Sulfate reducing bacteria+methanogen, g. hydrolysed ferments bacterium, hydrogen-producing acetogens+sulfate reducing bacteria+methanogen.
4. a kind of method improving methane output using origin bacterium as claimed in claim 2, which is characterized in that the step
(2) include the following steps：
I, prepares the coal seam sample in laboratory, using target coal sample, carries out the ultrasonic wave-coupled supercritical CO2Processing, into
Enter the laboratory operation management system, determines the ultrasonic wave-coupled supercritical CO2The best experiment item in processing experiment coal seam
Part, including temperature, pressure, time；
II, is overcritical to the target coal seam progress ultrasonic wave-coupled according to the temperature, pressure, time determined in the step I
CO2Partial organic substances in coal are dissolved in processing, extraction, improve coal seam constitution pores and crack, promote methane desorption；
III, collects the methane of output in production wellhead, to reduce coal seam pressure.
5. a kind of method improving methane output using origin bacterium as described in claim 1, which is characterized in that the step
(3) include the following steps：
I, detects gas componant and the concentration variation of production wellhead, using coal bed gas well routine bore mode by the step (1)
The zymotic fluid of middle acquisition is slowly injected into the target coal seam handled through the step (2), and timely sealing of hole；
II, is exploited the methane of production simultaneously by way of drainage and step-down mining when methane concentration meets using condition
It utilizes.
6. a kind of method improving methane output using origin bacterium as described in claim 1, which is characterized in that the step
(1) it uses clone library technology to analyze methanogen group structure, passes through qPCR technology quantitative analysis strains.
7. a kind of method improving methane output using origin bacterium as described in claim 1, which is characterized in that the step
(1) anaerobic fermentation bacterium is added in each combination in the step III in, enhances the palliating degradation degree of coal, and taking by various combination
The best bacterium colony of target coal seam aerogenesis is combined with determining, while avoiding the inhibiting effect of ecotone.
8. a kind of method improving methane output using origin bacterium as claimed in claim 4, which is characterized in that the data
Collection module carries out the collection of the pressure by pressure sensor, and the pressure sensor quantity is more than or equal to three；Institute
The data for stating the pressure sensor each time that data processing module acquires the data collection module are screened, then pass through institute
The average value for stating the pressure data of pressure sensor acquisition is P, then the calculation formula of the pressure mean values P is：
In formula, WiCalculation formula be：
In formula, P is the average value of pressure data, and i, j are respectively the serial number of the pressure sensor, Pi、PjRespectively i-th, j
The pressure that a pressure sensor obtains, n are the pressure sensor quantity, WiFor pressure value PiCorresponding coefficient value, NiFor pressure
Value PiCorresponding intermediate value.
9. a kind of method improving methane output using origin bacterium as claimed in claim 4, which is characterized in that the ultrasound
Wave couples supercritical CO2The treatment conditions of processing include ultrasonication condition and supercritical CO2Treatment conditions, the ultrasonic wave
Treatment conditions are：Pressure 0.5-2.5Mpa, processing time 10-60min, the supercritical CO2Treatment conditions are：Temperature with it is described
Target coal seam is consistent, pressure 7.4-9.6MPa, processing time 6h-54h.
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| CN107475118A (en) * | 2017-07-21 | 2017-12-15 | 山西晋城无烟煤矿业集团有限责任公司 | A kind of coal geomicrobiology flora passes on method for preserving |
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| CN107387044B (en) * | 2017-08-22 | 2019-12-17 | 太原理工大学 | Method for improving biological coal bed gas yield by using coal bed indigenous fungi |
| CN107576763B (en) * | 2017-10-13 | 2023-11-28 | 辽宁工程技术大学 | By means of ultrasound and high-temperature CO 2 Experimental device and method for promoting coal bed gas extraction |
| CN111271034A (en) * | 2020-01-19 | 2020-06-12 | 山西大学 | Method for improving biological coal bed gas yield by inducing lower aliphatic alcohol |
| CN111855377B (en) * | 2020-07-28 | 2023-08-08 | 太原理工大学 | Supercritical CO 2 Test device and method for methane production by coupling biological reaction of extracted coal |
| CN112922599B (en) * | 2021-04-08 | 2022-02-08 | 中国矿业大学 | Biological-high temperature gasification combined mining method for hydrogen production from coal |
| CN113738322B (en) * | 2021-09-01 | 2022-04-26 | 中国矿业大学 | Method for changing coal permeability by using hydrogen-producing acetogenic bacteria |
| CN114196709A (en) * | 2021-12-17 | 2022-03-18 | 西安科技大学 | Method for decomposing residual carbon based on methane bacteria in coal gasification underground space |
| CN114958924A (en) * | 2022-05-31 | 2022-08-30 | 中国矿业大学 | Coal-based biomass dark fermentation hydrogen production method with function of coal bed flora remodeling |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02307599A (en) * | 1989-05-22 | 1990-12-20 | Pub Works Res Inst Ministry Of Constr | Anaerobic digestion |
| CN101173604A (en) * | 2007-11-16 | 2008-05-07 | 中国科学院武汉岩土力学研究所 | Method for horizontal well mixed gas displacing coal-bed gas |
| CN102559772A (en) * | 2012-02-16 | 2012-07-11 | 北京科技大学 | Method for increasing production of coal bed methane by using exogenous microorganisms |
| CN104295276A (en) * | 2014-07-29 | 2015-01-21 | 太原理工大学 | Method for improving coalbed methane collection rate |
-
2016
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02307599A (en) * | 1989-05-22 | 1990-12-20 | Pub Works Res Inst Ministry Of Constr | Anaerobic digestion |
| CN101173604A (en) * | 2007-11-16 | 2008-05-07 | 中国科学院武汉岩土力学研究所 | Method for horizontal well mixed gas displacing coal-bed gas |
| CN102559772A (en) * | 2012-02-16 | 2012-07-11 | 北京科技大学 | Method for increasing production of coal bed methane by using exogenous microorganisms |
| CN104295276A (en) * | 2014-07-29 | 2015-01-21 | 太原理工大学 | Method for improving coalbed methane collection rate |
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