CN105385588A - Coal biological flora migration simulation experiment set and method - Google Patents
Coal biological flora migration simulation experiment set and method Download PDFInfo
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- C12M23/58—Reaction vessels connected in series or in parallel
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Abstract
The invention discloses a coal biological flora migration simulation experiment set and method. The set comprises a coal bed biology aerogenesis system, a strain migration and observation system and an experiment control and record system, wherein the coal bed biology aerogenesis system and the strain migration and observation system are a first box and a second box which are connected through a coaling pipe, and migration and observation can only be conducted on one coal sample during each time of work. Due to the fact that the initial liquid level of the first box is identical with that of the second box and gas generated after coal reacts with microorganisms can be discharged in time, flora diffusion is not affected by external force basically, and it is guaranteed that microorganisms are freely diffused through preset coal samples. By testing the indexes including identification of flora contained in liquid on the two sides of the unique microorganism migration channel, namely the coaling pipe, gas production rate, pH and Eh, the migration and diffusion rules of biological flora in different coal reservoirs are researched.
Description
Technical field
The invention belongs to coal-seam gas technical field of bioengineering, particularly relate to biological flora Migration Simulation experimental installation and method in a kind of coal.
Background technology
Biogenesis coal-seam gas is an important kind of coal-seam gas, buries the features such as shallow, cost of development is low.At present, the Basic quota of biogenesis coal-seam gas, particularly secondary biogenic gas coal-seam gas and exploratory development have become the focus of coal-seam gas geology circle research.Basin, U.S.'s powder river cbm exploration stage content only 1 ~ 2m
3/ t, but current coal bed gas well breaks through 20000 mouthfuls, and per-well production is at 2000 ~ 3000m
3.The major part extraction well of more than 5 years, the coal-seam gas total amount of output, considerably beyond the substantial resource amount that the exploration stage calculates, illustrates the generation still having large number of biological gas at present, generates and be drawn out of, lasting generation, lasting air feed.Australia has also carried out the Pilot trial of coal seam biogasification.Coal-seam gas biotechnology all plays an important role to realizing energy-saving and emission-reduction, increasing stock number and improving reservoir permeability etc., is a direction of following coal-seam gas industry development.Chinese scholars mainly concentrates on formation mechanism, the aspect such as formation condition and influence factor of biogenetic gas for the research of biological methane, but rarely has the migration of microorganism species in coal and Diffusion Law and relate to.
Summary of the invention
The present invention, in order to solve weak point of the prior art, provides a kind of compact construction, is easy to assemble, can simulate biological flora Migration Simulation experimental installation and method in the coal of biological flora migration effect in coal.
For solving the problems of the technologies described above, the present invention adopts following technical scheme: biological flora Migration Simulation experimental installation in coal, comprises the biological gas production system in coal seam, bacterial classification migration and recording geometry and experiment control and register system;
The biological gas production system in coal seam comprises constant water bath box, and be placed with the first casing being loaded with pit water enrichment 4-6 days bacterium liquid and the second casing being loaded with sterilized water in constant water bath box, the first casing is positioned on the right side of the second casing; First casing lower right side is provided with the first liquid flowing tube, first liquid flowing tube is provided with the first liquid taking valve, first casing top right side is provided with the first liquid injection tube extend in the first casing, first liquid injection tube is provided with the first liquid-filling valve be positioned at above the first casing, and the first casing left lower is provided with to demote and moves mouth; Second casing left lower is provided with the second liquid flowing tube, second liquid flowing tube is provided with the second liquid taking valve, second casing top left side is provided with the second liquid injection tube extend in the second casing, second liquid injection tube is provided with the second liquid-filling valve be positioned at above the second casing, and the second casing lower right side is provided with right migration mouth;
Bacterial classification migration comprises horizontally disposed coalingging manage with recording geometry, is filled with coal sample in pipe of coalingging, and pipe left end of coalingging moves mouth and is connected with demoting, and pipe right-hand member of coalingging is connected with right migration mouth, demotes to move Kou Chu and be provided with left filter screen, and right migration mouth place is provided with right filter screen;
Experiment control and register system comprise computer, left bank water gas extractor, right draining gas extractor, left pH sensor, left Eh sensor, left gas flow sensor, right pH sensor, right Eh sensor and right gas flow sensor, computer by Data Control line respectively with left pH sensor, left Eh sensor, left gas flow sensor, right pH sensor, right Eh sensor is connected with right gas flow sensor, left pH sensor and left Eh sensor to extend in the second casing under bacterium liquid liquid level, right pH sensor and right Eh sensor to extend in the first casing under bacterium liquid liquid level, left bank water gas extractor is communicated with the second box house by left effuser, and left gas flow sensor is located on left effuser, and right draining gas extractor is communicated with the first box house by right effuser, and right gas flow sensor is located on right effuser.
Left bank water gas extractor is identical with the structure of right draining gas extractor;
Left bank water gas extractor comprises left air collector and left overflow bottle, water is loaded with in left air collector, left effuser extend into above the water surface in left air collector, is connected between left overflow bottle and left air collector by left upflow tube, and left upflow tube extend into the underwater in left air collector;
Right draining gas extractor comprises right air collector and right overflow bottle, water is loaded with in right air collector, right effuser extend into above the water surface in right air collector, is connected between right overflow bottle and right air collector by right upflow tube, and right upflow tube extend into the underwater in right air collector.
Left effuser is provided with left gas collection valve, and right effuser is provided with right gas collection valve.
Biological methane flora Migration Simulation experimental technique in coal, comprises the following steps:
(1), assemble the biological gas production system in above-mentioned coal seam, bacterial classification migration and recording geometry and experiment control and register system, test the resistance to air loss of whole experimental installation;
(2), in constant water bath box water is poured into, the temperature of the water temperature simulation coal seam reservoirs in setting constant water bath box;
(3), in the coal sample loading simulation coal seam reservoirs in pipe of coalingging, move a mouthful place left filter screen is set demoting, right filter screen is set at right migration mouth place, to prevent coal sample unrestrained;
(4) the first liquid taking valve and the second liquid taking valve, is closed, sterilized water is injected in bacterium liquid after injecting enrichment by the first liquid injection tube and the second liquid injection tube respectively to the first casing and the second casing, close the first liquid-filling valve and the second liquid-filling valve, open right gas collection valve and left gas collection valve;
, etc. (5) in the first casing and the second casing after level balance, start left pH sensor, left Eh sensor, left gas flow sensor, right pH sensor, right Eh sensor and right gas flow sensor, open the first liquid taking valve at set intervals and the second liquid taking valve carries out getting liquid, flora contained in the liquid taken out is identified;
(6), test and terminate, site clearing salvage material, contrast pH, Eh in different time first casing and the second casing, Flora dynamics and gas production rate index.
Open the first liquid taking valve and the second liquid taking valve in step (5) while, take out and to coaling pipe, coaling manage in different positions to take a morsel coal sample, types of spawn contained by it and concentration are identified.
Adopt technique scheme, the biological gas production system in coal seam, bacterial classification migration are manage the first casing and the second casing that are connected by coalingging with recording geometry, can only carry out migration observation when carrying out operation to a kind of coal sample at every turn.Because the first casing is identical with the initial level in the second casing, and the gas that coal and microbial process generate can be discharged in time, and therefore the diffusion of flora is not substantially by External Force Acting, ensure that the coal sample free diffusing that microorganism passes through to preset.By unique passage that microorganism moves, the index tests such as Bacteria identification, gas production rate, pH and Eh contained by the liquid of pipe both sides of namely coalingging, study biological flora migration and Diffusion Law in different coal seam reservoirs.
Present invention achieves and comprehensively analyze biological flora diffusion mobility process in coal at a device.The present invention is under the envrionment conditions of setting, and utilize coal seam origin bacterium to carry out biological aerogenesis simulated experiment, in gas generation process, microorganism species carries out moving and spreading, and utilizes aerogenesis and strain identification characterising biological flora transition process.
Compact construction of the present invention, be easy to assembling, can simulate biological flora migration effect in coal, at guarantee microorganism species by free migration under External Force Acting, the flora diffusion for research coal-seam gas bioengineering field provides well tests support.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Embodiment
As shown in Figure 1, biological flora Migration Simulation experimental installation in coal of the present invention, comprises the biological gas production system in coal seam, bacterial classification migration and recording geometry and experiment control and register system.
The biological gas production system in coal seam comprises constant water bath box 1, is placed with the first casing 2 being loaded with pit water enrichment 4-6 days bacterium liquid and is positioned on the right side of the second casing 3 with the second casing 3, first casing 2 being loaded with sterilized water in constant water bath box 1; First casing 2 lower right side is provided with the first liquid flowing tube 4, first liquid flowing tube 4 is provided with the first liquid taking valve 5, first casing 2 top right side is provided with the first liquid injection tube 6 extend in the first casing 2, first liquid injection tube 6 is provided with the first liquid-filling valve 7, first casing 2 left lower be positioned at above the first casing 2 and is provided with to demote and moves mouth; Second casing 3 left lower is provided with the second liquid flowing tube 8, second liquid flowing tube 8 is provided with the second liquid taking valve 9, second casing 3 top left side is provided with the second liquid injection tube 10 extend in the second casing 3, second liquid injection tube 10 is provided with the second liquid-filling valve 11, second casing 3 lower right side be positioned at above the second casing 3 and is provided with right migration mouth.
Bacterial classification migration comprises horizontally disposed pipe 12 of coalingging with recording geometry, coaling in pipe 12 and be filled with coal sample 13, pipe 12 left end of coalingging moves mouth and is connected with demoting, and pipe 12 right-hand member of coalingging is connected with right migration mouth, demote and move Kou Chu and be provided with left filter screen 14, right migration mouth place is provided with right filter screen 15.
Experiment control and register system comprise computer 16, left bank water gas extractor, right draining gas extractor, left pH sensor 17, left Eh sensor 18, left gas flow sensor 19, right pH sensor 20, right Eh sensor 21 and right gas flow sensor 22, computer 16 by Data Control line respectively with left pH sensor 17, left Eh sensor 18, left gas flow sensor 19, right pH sensor 20, right Eh sensor 21 is connected with right gas flow sensor 22, left pH sensor 17 and left Eh sensor 18 to extend in the second casing 3 under bacterium liquid liquid level, right pH sensor 20 and right Eh sensor 21 to extend in the first casing 2 under bacterium liquid liquid level, left bank water gas extractor is communicated with the second casing 3 inside by left effuser 23, left gas flow sensor 19 is located on left effuser 23, right draining gas extractor is communicated with the first casing 2 inside by right effuser 24, and right gas flow sensor 22 is located on right effuser 24.
Left bank water gas extractor is identical with the structure of right draining gas extractor; Left bank water gas extractor comprises left air collector 25 and left overflow bottle 26, water is loaded with in left air collector 25, left effuser 23 extend into above the water surface in left air collector 25, connected by left upflow tube 27 between left overflow bottle 26 and left air collector 25, left upflow tube 27 extend into the underwater in left air collector 25.
Right draining gas extractor comprises right air collector 28 and right overflow bottle 29, water is loaded with in right air collector 28, right effuser 24 extend into above the water surface in right air collector 28, connected by right upflow tube 30 between right overflow bottle 29 and right air collector 28, right upflow tube 30 extend into the underwater in right air collector 28.
Left effuser 23 is provided with left gas collection valve 31, and right effuser 24 is provided with right gas collection valve 32.
Biological methane flora Migration Simulation experimental technique in coal, comprises the following steps:
(1), assemble the biological gas production system in above-mentioned coal seam, bacterial classification migration and recording geometry and experiment control and register system, test the resistance to air loss of whole experimental installation;
(2), in constant water bath box 1 water is poured into, the temperature of the water temperature simulation coal seam reservoirs in setting constant water bath box 1;
(3), in the coal sample 13 loading simulation coal seam reservoirs in pipe 12 of coalingging, move a mouthful place left filter screen 14 is set demoting, right filter screen 15 is set at right migration mouth place, trickles down to prevent coal sample 13;
(4) the first liquid taking valve 5 and the second liquid taking valve 9, is closed, injection sterilized water in the bacterium liquid after enrichment and the second casing 3 is injected respectively to the first casing 2 by the first liquid injection tube 6 and the second liquid injection tube 10, close the first liquid-filling valve 7 and the second liquid-filling valve 11, open right gas collection valve 32 and left gas collection valve 31;
, etc. (5) in the first casing 2 and the second casing 3 after level balance, start left pH sensor 17, left Eh sensor 18, left gas flow sensor 19, right pH sensor 20, right Eh sensor 21 and right gas flow sensor 22, open the first liquid taking valve 5 at set intervals and the second liquid taking valve 9 carries out getting liquid, flora contained in the liquid taken out is identified;
(6), test and terminate, site clearing salvage material, contrast pH, Eh in different time first casing 2 and the second casing 3, Flora dynamics and gas production rate index.
Open the first liquid taking valve 5 and the second liquid taking valve 9 in step (5) while, take out and to coaling pipe 12, to take a morsel coal sample 13 at different positions in pipe 12 of coalingging, types of spawn contained by it and concentration are identified.
The present embodiment not does any pro forma restriction to shape of the present invention, material, structure etc.; every above embodiment is done according to technical spirit of the present invention any simple modification, equivalent variations and modification, all belong to the protection domain of technical solution of the present invention.
Claims (5)
1. biological flora Migration Simulation experimental installation in coal, is characterized in that: comprise the biological gas production system in coal seam, bacterial classification migration and recording geometry and experiment control and register system;
The biological gas production system in coal seam comprises constant water bath box, and be placed with the first casing being loaded with pit water enrichment 4-6 days bacterium liquid and the second casing being loaded with sterilized water in constant water bath box, the first casing is positioned on the right side of the second casing; First casing lower right side is provided with the first liquid flowing tube, first liquid flowing tube is provided with the first liquid taking valve, first casing top right side is provided with the first liquid injection tube extend in the first casing, first liquid injection tube is provided with the first liquid-filling valve be positioned at above the first casing, and the first casing left lower is provided with to demote and moves mouth; Second casing left lower is provided with the second liquid flowing tube, second liquid flowing tube is provided with the second liquid taking valve, second casing top left side is provided with the second liquid injection tube extend in the second casing, second liquid injection tube is provided with the second liquid-filling valve be positioned at above the second casing, and the second casing lower right side is provided with right migration mouth;
Bacterial classification migration comprises horizontally disposed coalingging manage with recording geometry, is filled with coal sample in pipe of coalingging, and pipe left end of coalingging moves mouth and is connected with demoting, and pipe right-hand member of coalingging is connected with right migration mouth, demotes to move Kou Chu and be provided with left filter screen, and right migration mouth place is provided with right filter screen;
Experiment control and register system comprise computer, left bank water gas extractor, right draining gas extractor, left pH sensor, left Eh sensor, left gas flow sensor, right pH sensor, right Eh sensor and right gas flow sensor, computer by Data Control line respectively with left pH sensor, left Eh sensor, left gas flow sensor, right pH sensor, right Eh sensor is connected with right gas flow sensor, left pH sensor and left Eh sensor to extend in the second casing under bacterium liquid liquid level, right pH sensor and right Eh sensor to extend in the first casing under bacterium liquid liquid level, left bank water gas extractor is communicated with the second box house by left effuser, and left gas flow sensor is located on left effuser, and right draining gas extractor is communicated with the first box house by right effuser, and right gas flow sensor is located on right effuser.
2. biological flora Migration Simulation experimental installation in coal according to claim 1, is characterized in that: left bank water gas extractor is identical with the structure of right draining gas extractor;
Left bank water gas extractor comprises left air collector and left overflow bottle, water is loaded with in left air collector, left effuser extend into above the water surface in left air collector, is connected between left overflow bottle and left air collector by left upflow tube, and left upflow tube extend into the underwater in left air collector;
Right draining gas extractor comprises right air collector and right overflow bottle, water is loaded with in right air collector, right effuser extend into above the water surface in right air collector, is connected between right overflow bottle and right air collector by right upflow tube, and right upflow tube extend into the underwater in right air collector.
3. biological flora Migration Simulation experimental installation in coal according to claim 2, it is characterized in that: left effuser is provided with left gas collection valve, right effuser is provided with right gas collection valve.
4. biological methane flora Migration Simulation experimental technique in coal according to claim 3, is characterized in that: comprise the following steps:
(1), assemble the biological gas production system in above-mentioned coal seam, bacterial classification migration and recording geometry and experiment control and register system, test the resistance to air loss of whole experimental installation;
(2), in constant water bath box water is poured into, the temperature of the water temperature simulation coal seam reservoirs in setting constant water bath box;
(3), in the coal sample loading simulation coal seam reservoirs in pipe of coalingging, move a mouthful place left filter screen is set demoting, right filter screen is set at right migration mouth place, to prevent coal sample unrestrained;
(4) the first liquid taking valve and the second liquid taking valve, is closed, sterilized water is injected in bacterium liquid after injecting enrichment by the first liquid injection tube and the second liquid injection tube respectively to the first casing and the second casing, close the first liquid-filling valve and the second liquid-filling valve, open right gas collection valve and left gas collection valve;
, etc. (5) in the first casing and the second casing after level balance, start left pH sensor, left Eh sensor, left gas flow sensor, right pH sensor, right Eh sensor and right gas flow sensor, open the first liquid taking valve at set intervals and the second liquid taking valve carries out getting liquid, flora contained in the liquid taken out is identified;
(6), test and terminate, site clearing salvage material, contrast pH, Eh in different time first casing and the second casing, Flora dynamics and gas production rate index.
5. biological methane flora Migration Simulation experimental technique in coal according to claim 4, it is characterized in that: open the first liquid taking valve and the second liquid taking valve in step (5) while, taking-up is coaling pipe, coalingging, different positions in pipe takes a morsel coal sample, identifies types of spawn contained by it and concentration.
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| CN105838598A (en) * | 2016-05-31 | 2016-08-10 | 河南理工大学 | Simulation experiment device for biomethanation of carbon dioxide sequestered in coal seams and experiment method |
| CN114166969A (en) * | 2021-11-30 | 2022-03-11 | 西安科技大学 | Reaction device for simulating microorganisms to metabolize combustible gas in coal and test method thereof |
| CN117187050A (en) * | 2023-11-08 | 2023-12-08 | 中国煤炭地质总局勘查研究总院 | Experimental device for researching coalbed methane biological injection and migration rules |
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