CN105861277A - Underwater injection-type sediment in-situ culture device - Google Patents
Underwater injection-type sediment in-situ culture device Download PDFInfo
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- CN105861277A CN105861277A CN201610249655.1A CN201610249655A CN105861277A CN 105861277 A CN105861277 A CN 105861277A CN 201610249655 A CN201610249655 A CN 201610249655A CN 105861277 A CN105861277 A CN 105861277A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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Abstract
The invention relates to an underwater injection-type sediment in-situ culture device. A basic principle of the underwater injection-type sediment in-situ culture device is that culture liquid is injected in advance into an injector in the culture device; a submersible vehicle carries the culture device to a deep seabed, a manipulator holds a T-shaped handle to firstly inert the culture device into sediment and then pull a knot at an upper end of a cable to pull out a cotter, an arc-shaped baffle rotates around a bolt and slides away under the effect of spring force, a lower baffle presses a push rod of the injector under the effect of the spring force, and the push rod moves downwards to inject the culture liquid into sediment at different depths in a culture cylinder to carry out in-situ culture. The underwater injection-type sediment in-situ culture device can simultaneously realize in-situ culture of 1 to 10 types of culture liquid at 1 to 10 culture depths in deep seabed sediment. The culture device can cooperatively perform operation with an ROV (Remotely Operated Vehicle) and an HOV (Human Operated Vehicle), the principle is reliable, the structure is simple, the operation is facilitated, and the culture device is suitable for in-situ culture of sediment on the deep seabed.
Description
Technical field
The invention belongs to marine survey technology field, be specifically related to one injection deposit Culture in situ device under water,
By injecting the various culture fluid such as isotopic tracer in different depth deposit at deep seafloor, it is achieved the original position of deposit
Cultivate and sample collecting.
Background technology
The research of abyssopelagic organism geochemical process is significant for understanding carbon nitrogen sulfur cycle.Traditional biological geochemistry
The research majority of process is to gather deposit in seabed, is then transported on ship, aboard ship in laboratory or land laboratory
The sediment sample collected adds the various culture fluid such as isotopic tracer cultivate, open finally by cultured products
Exhibition is analyzed test and is disclosed speed and the mechanism of the biogeochemical reaction that Institute of Micro-biology mediates.But in the pole such as hydrothermal solution and cold spring
, there is the material such as methane/hydrogen/hydrogen sulfide of high concentration in end environments such as subsea, these materials are that chemosynthetic microbe existence must
The energy needed.The gas of these high concentrations contained in deposit from seabed collection and transports deck at sediment sample subsequently
Lengthy procedure in, can cause collecting in the sediment sample on deck because the change of temperature, pressure occurs exchange and escapes
The content of this kind of gas substantially reduces, thus notable deviation occurs in the result causing laboratory cultures to test.
At present can be with the deposit short cylinder sampler of submersible supporting operation, it is heavy to be gathered in seabed by mechanical hand
Long-pending thing sample, is unable to carry the culture fluid such as isotopic tracer and carries out Culture in situ experiment in bottom sediment.This
The deposit Culture in situ device of injection under water of bright development, can according to Research Requirements, will cultivate required for isotope show
The culture fluid such as track agent are poured in the syringe in device in advance, submersible culture apparatus is carried to deep seafloor, then
Under the operation of mechanical hand, select to cultivate the Culture in situ of place development deposit in seabed, cultivate and will acquire again after terminating
After cultivation, the Culture in situ device of deposit is taken back on ship.
Summary of the invention
It is an object of the invention to provide that a kind of principle is reliable, simple in construction, injection deposit under water convenient to operate
Culture in situ device.This device can be with underwater remote-control submersible ROV or manned underwater vehicle HOV supporting operation, at deep seafloor
Deposit is carried out Culture in situ and the sample collecting of different depth.During actual application, can be according to Research Requirements, by different trainings
Support the culture fluid such as substrate or isotopic tracer to be previously charged in syringe, and according to cultivating depth requirements, difference length is installed
The syringe needle of degree, it is achieved the Culture in situ of bottom sediment different depth.
The deposit Culture in situ device of injection under water that the present invention proposes, including cable 1, T-shaped screw mechanism, triggering machine
Structure, syringe 11a and supporting syringe needle 11b, perforated baffle 12, silica gel pad 13, housing 14 and cultivation cylinder 19, wherein:
T-shaped screw mechanism includes that T-shaped screw rod the 2, first spring adjusting nut 3a, the second spring adjusting nut 3b, silica gel pad are tight
Gu nut 3c, the first screw rod clamp nut 3d, the second screw rod clamp nut 3e, overhead gage 4, lower baffle plate 10 and spring 5, T-shaped spiral shell
The screw rod end of bar 2 sequentially passes through the first spring adjusting nut 3a, the second spring adjusting nut 3b, overhead gage 4, spring from top to bottom
5, lower baffle plate 10, silica gel pad clamp nut 3c, silica gel pad 13, perforated baffle 12 and housing 14, spring 5 is positioned at overhead gage 4
And between lower baffle plate 10, the first spring adjusting nut 3a, the second spring adjusting nut 3b, overhead gage 4, spring 5 and lower baffle plate 10
As the actuating unit of culture apparatus, during triggering, make pushing away of lower baffle plate 10 stress down pressure injection emitter by the spring force of spring 5
Bar, thus the injection irrigated in syringe is injected in deposit；By degree of tightness the first spring adjusting nut 3a and second
Spring adjusting nut 3b, can regulate the pre compressed magnitude of spring, thus control the size of spring force；First spring adjusting nut
3a, the use of the second spring adjusting nut 3b may also function as locking, fastening effect；Housing 14 and T-shaped screw rod 2 junction are passed through
First screw rod clamp nut 3d, the second screw rod clamp nut 3e fastening；
Trigger mechanism includes the first block arc 6a, the second block arc 6b, the first bolt 7a, the second bolt 7b, the 3rd bolt
7c, split pin 8 and connection sheet 9, the upper end of the first block arc 6a and the second block arc 6b is equipped with snap ring；First arc gear
Block 6a is provided with through hole, and described through hole is used for connecting split pin 8, the first block arc 6a and the second block arc 6b relative to cloth
Putting, constitute similar circular ring structure, the first block arc 6a, perforated baffle 12 and housing 14 are connected by the first bolt 7a, and second
Block arc 6 b, perforated baffle 12 and housing 14 are connected by the second bolt 7b；
Cultivation cylinder 19 is set bottom housing 14；It is evenly arranged several syringes installation passage 15b on housing 14 and draining has been led to
Road 16b；
Syringe 11a one end sequentially passes through perforated baffle 12, housing 14, stretches in cultivation cylinder 19；
Cable 1 one end connects the first block arc 6a；
Silica gel pad 13 is installed on above perforated baffle 12, by regulating the position of silica gel pad clamp nut 3c, makes silicagel pad
Sheet 13 is pressed on perforated baffle 12, blocks the drainage channel 16b of the osculum 16a on perforated baffle 12 and housing 14；Work as training
When supporting in device insertion deposit, current can be upwardly through the drainage channel 16b on housing 14 and the draining on perforated baffle 12
Hole 16a discharges；When cultivation completes, culture apparatus is taken out from seabed rear silica gel pad 13 and may also function as preventing extraneous sea water from entering
Enter deposit and cultivate the effect of cylinder 19.
In the present invention, when the through hole on the first block arc 6a is inserted in split pin 8, the 3rd bolt 7c precession the second arc
In the screwed hole arranged on block 6b, the 3rd bolt 7c one end connects split pin 8 by connecting sheet 9, so that the first arc gear
The second snap ring 6d of the first snap ring 6c and the second block arc 6b of block 6a blocks lower baffle plate 10 and is allowed to can not continue down；When opening
When mouth pin 8 is not installed, the first block arc 6a can be freely rotatable by the first bolt 7a.
In the present invention, a knot is made a call in cable 1 upper end, facilitates the supporting operation of submersible mechanical hand.
In the present invention, during use, cable 1 and split pin 8 trigger device action, when at deep seafloor submersible mechanical hand
During the knot of pull-up cable 1 upper end, split pin 8 will be taken advantage of a situation and be pulled out, and make curved baffle 6a rotate around bolt 7a and slip off, lower baffle plate
10 under the active force of spring 5, is pressed downward against the push rod of syringe 11a, and push rod moves the culture fluid that pours in advance along syringe
Syringe needle 11b injects in the deposit cultivated in cylinder 19.
In the present invention, cultivate cylinder 19 and can select the transparent organic glass cylinder of different size according to actual needs, by waterproof
Adhesive tape can be bonded in the lower end of housing 14, and fixes by stainless steel lathe dog.
The present invention can install and use 1-10 and prop up syringe 11a, and the syringe needle of supporting different length according to actual needs
Head 11b, to realize the cultivation of seabed different depth deposit.During actual installation, need to shift to an earlier date to inject in syringe 11a and cultivate
Liquid, adjusts the height of push rod according to actual injection amount, and the syringe that then syringe inserts housing 14 is installed in passage 15b,
And push down injector cartridge with perforated baffle 12.
The beneficial effects of the present invention is the deposition achieving 1-10 different depth of deep seafloor in the way of purely mechanic
Thing Culture in situ, can add 1-10 kind culture fluid according to demand in deposit.Apparatus structure is simple, and principle is reliable, user
Just, suitably at deep seafloor, deposit is carried out Culture in situ and sample collecting.
Accompanying drawing explanation
Fig. 1 is present configuration decomposing schematic representation.
Fig. 2 is installation assembly front schematic view of the present invention.
Fig. 3 is installation assembly reverse side schematic diagram of the present invention.
Fig. 4 is curved baffle schematic diagram of the present invention.
Fig. 5 is snap ring sectional view of the present invention.
Fig. 6 is inventive shell 14 sectional view.A () is front view, (b) is right view, and (c) is top view.
Label in figure: 1 is cable, 2 is T-shaped screw rod, and 3a, 3b respectively first, second spring adjusting nut, 3c are silica gel
Pad clamp nut, 3d, 3e are respectively first, second screw rod clamp nut, and 4 is overhead gage, and 5 is spring, and 6a, 6b are respectively
One, the second block arc, 6c and 6d is respectively first, second snap ring, and 7a, 7b, 7c are respectively first, second, and third bolt, 8
For split pin, 9 for connecting sheet, and 10 is lower baffle plate, and 11a is syringe, and 11b is syringe needle, and 12 is perforated baffle, and 13 is silicon
Glue gasket, 14 is housing, and 15a is syringe through hole, and 15b is that syringe installs passage, and 16a is osculum, and 16b is that draining is led to
Road, 17a is that T-shaped screw rod installs through hole, and 17b is that T-shaped screw rod installs passage, and 18a, 18b are bolt hole, and 18c, 18d are respectively
First, second internal thread hole, 19 for cultivating cylinder.
Detailed description of the invention
Combine accompanying drawing below by embodiment and further illustrate the present invention.
Embodiment 1:
As shown in figures 1 to 6, injection deposit Culture in situ device includes cable 1, T-shaped screw mechanism, trigger mechanism, note under water
Emitter 11a and supporting syringe needle 11b, perforated baffle 12, silica gel pad 13, housing 14 and cultivation cylinder 19.Cable 1 upper end makes a call to one
Knot, facilitates the supporting operation of submersible mechanical hand.
T-shaped screw mechanism includes T-shaped screw rod the 2, first spring adjusting nut 3a and the second spring adjusting nut 3b, silicagel pad
Sheet clamp nut 3c, T-shaped screw rod clamp nut 3d and 3e, overhead gage 4, lower baffle plate 10, spring 5.Wherein double nut 3a and 3b, on
Baffle plate 4, spring 5, lower baffle plate 10 are as the actuating unit of device.During triggering, make lower baffle plate 10 stress by the spring force of spring
The down push rod of pressure injection emitter, thus the injection irrigated in syringe is injected in deposit.By degree of tightness double nut 3a
And 3b, the pre compressed magnitude of spring can be regulated, thus control the size of spring force.The use of double nut may also function as anti-loosening, tighten
Gu effect.
Trigger mechanism includes block arc 6a and 6b, bolt 7a and 7b and 7c, split pin 8, connects sheet 9.Block arc 6a and 6b,
It is characterized in that upper end is respectively arranged with one section of snap ring 6c and 6d；Two through hole connecting bolt 7a and split pin 8 is had on block arc 6a,
A through hole connecting bolt 7b, an internal thread hole connecting bolt 7c is had on block arc 6b.
Uniformly having laid 10 syringes on housing 14 and installed passage 15b, 10 drainage channel 16b, center is T-shaped screw rod
Passage 17b is installed, by this passage, screw rod 2 can be injected in housing 14, and fasten with screw rod clamp nut 3d and 3e in lower end.
Internal thread hole 18c and 18d is then the installing hole of bolt 7a and 7b.
Be T-shaped screw rod installation through hole 17a in the middle of perforated baffle 12, distributed about have syringe through hole 15a, osculum 16a,
Bolt hole 18a and 18b, they respectively with T-shaped screw rod install passage 17b, syringe install passage 15b, drainage channel 16b,
The position one_to_one corresponding of internal thread hole 18c and 18d.
Silica gel pad 13 is arranged on above perforated baffle 12, can make silicon by the position of regulation silica gel pad clamp nut 3c
Glue gasket 13 is pressed on perforated baffle 12, blocks the drainage channel 16b of the osculum 16a on perforated baffle 12 and housing 14.
When culture apparatus inserts in deposit, current can be discharged upwardly through drainage channel 16b and osculum 16a；When having cultivated
Become, culture apparatus is taken out from seabed the work that rear silica gel pad 13 may also function as preventing extraneous sea water from entering deposit cultivation cylinder 19
With.
In trigger mechanism, block arc 6a and 6b, perforated baffle 12 have been connected by use bolt 7a and 7b with housing 14
Come.Bolt 7a and 7b inserts the through hole on block arc 6a and 6b, then pass through the bolt hole 18a on perforated baffle 12 and
18b, internal thread hole 18c and 18d on last precession housing 14.Connecting after having fixed, perforated baffle presses syringe cylinder 12 times
Body 11a.The through hole on block arc 6a is inserted in split pin 8, in the screwed hole on bolt 7c precession block arc 6b, and by even
Contact pin 9 is connected, so that snap ring 6c and 6d of block arc 6a and 6b upper end blocks lower baffle plate 10 and be allowed to can not continue down.
When split pin 8 is not installed, block arc 6a can be freely rotatable by bolt 7a.
The present invention uses the split pin 8 of cable 1 and lower end connection to trigger device action.When at deep seafloor submersible machine
When tool has pulled the knot of cable 1 upper end, split pin 8 will be taken advantage of a situation and be pulled out, and make curved baffle 6a rotate around bolt 7a and slip off, under
Baffle plate 10, under the active force of spring 5, is pressed downward against the push rod of syringe 11a, and push rod moves the culture fluid poured in advance along noting
Emitter syringe needle 11b injects in the deposit cultivated in cylinder 19.
Cultivate cylinder 19 and can select the transparent organic glass cylinder of different size according to actual needs, can be by it by adhesive waterproof tape
It is bonded in the lower end of housing 14, and fixes by stainless steel lathe dog.
Cultivate cylinder 19 and select the high intensity acrylic pipe of 50cm length, the syringe 11a two of 1ml, corresponding injection are installed
Device syringe needle is respectively 316 rustless steel syringe needles of 10cm and 20cm length.
1, culture apparatus is installed
1. prepare the culture fluid of a certain concentration, the syringe of two 1ml sucks 0.5ml culture fluid respectively, connect syringe
Syringe needle 11b also inserts the syringe of housing 14 and installs in passage 15b.At housing 14 upper ends perforated baffle 12, push down injection
Device 11a cylinder also makes push rod exposure from through hole 15a, and the position making each hole is corresponding with the position in hole on housing 14 identical.
2. silica gel pad 13 is placed in the position in perforated baffle 12 intermediate throughholes upper end, is allowed to block osculum 16a.
3. perforated baffle 12 upper end correct position install trigger mechanism, block arc 6a and 6b insert bolt 7a and
7b, make its lower end through after bolt hole 18a and 18b of perforated baffle respectively precession housing 14 upper end internal thread hole 18c and
18d.Internal thread hole precession bolt 7c on block arc 6b, and load connection sheet 9；Corresponding through hole position on block arc 6a
Put insertion split pin 8, be connected with bolt 7c by connecting sheet 9.Split pin 8 upper end connects a cable 1, and cable top is cable
Knot.
4. from two spring adjusting nut 3a and 3b of T-shaped screw rod bottom precession, be then sequentially loaded into overhead gage 4, spring 5,
Lower baffle plate 10.Load silicagel pad clamp nut 3c, T-shaped screw rod 2 lower end is stretched into successively in the middle of silica gel pad 13, perforated baffle 12
T-shaped screw rod installs through hole 17a, and the T-shaped screw rod being inserted into housing 14 is installed in passage 17b, and in T-shaped screw rod 2 lower end precession
Two screw rod clamp nut 3d and 3e, make lower baffle plate 10 be stuck on circumferential clasp 6c and 6d inside block arc 6a and 6b；Adjust
Joint nut 3c makes silica gel pad 13 be pressed on perforated baffle 12, and regulation spring adjusting nut 3a and 3b adjusts the pre-of spring 5
Clamp force so that it is when release, the culture fluid in syringe all can be injected in deposit.
5. cultivation cylinder 19 is injected housing 14 lower end, and blocks by stainless steel lathe dog after adhesive waterproof tape bonding, prevent training
Support cylinder 19 to come off from housing 14.
3, culture apparatus is not put and reclaims
Submersible object stage is installed fixing protection cylinder, the deposit Culture in situ device of injection under water assembled is inserted
Enter in supporting protection cylinder and fix, submersible being carried to seabed, using mechanical hand to hold T-shaped handle, by culture apparatus
Being placed on appointment position, lower pressure makes cultivation cylinder 19 be adequately inserted in bottom sediment, and the water cultivated in cylinder 19 can pass through housing 14
On drainage channel 16b and osculum 16a on perforated baffle 12 discharge.Subsequently, mechanical hand pulls the cable on cable 1 top
Knot, split pin 8 takes advantage of a situation and is pulled out, and makes curved baffle 6a rotate around bolt 7a and slips off, lower baffle plate 10 under spring force, to
The push rod of lower pressure injection emitter 11a, push rod is pressed down the injection of pushing syringe 11a to inject along needle tubing 11b and is cultivated difference in cylinder 19
In the deposit of the degree of depth.
4, after cultivating 24 hours, culture apparatus is extracted from bottom sediment by submersible mechanical hand, is placed into submersible
Protection cylinder supporting on object stage returns on ship with submersible.Period cannot be introduced into due to the existence of silica gel pad 13, sea water
Cultivate in cylinder 19, effectively prevent deposit and taken away cultivation cylinder 19 by sea water in moving process.
Claims (5)
1. an injection deposit Culture in situ device under water, including cable (1), T-shaped screw mechanism, trigger mechanism, injection
Device (11a) and supporting syringe needle (11b), perforated baffle (12), silica gel pad (13), housing (14) and cultivation cylinder (19), it is special
Levy and be: T-shaped screw mechanism includes T-shaped screw rod (2), the first spring adjusting nut (3a), the second spring adjusting nut (3b), silicon
Glue gasket clamp nut (3c), the first screw rod clamp nut (3d), the second screw rod clamp nut (3e), overhead gage (4), lower baffle plate
(10) and spring (5), the screw rod end of T-shaped screw rod (2) sequentially passes through the first spring adjusting nut (3a), the second spring from top to bottom
Adjusting nut (3b), overhead gage (4), spring (5), lower baffle plate (10), silica gel pad clamp nut (3c), silica gel pad (13),
Perforated baffle (12) and housing (14), spring (5) is positioned between overhead gage (4) and lower baffle plate (10), the first spring adjusting nut
(3a), the second spring adjusting nut (3b), overhead gage (4), spring (5) and lower baffle plate (10) are as the engine of culture apparatus
Structure, during triggering, makes the push rod of lower baffle plate (10) stress down pressure injection emitter by the spring force of spring (5), thus by syringe
The injection of middle perfusion is injected in deposit；By degree of tightness the first spring adjusting nut (3a) and the second spring adjusting nut
(3b), the pre compressed magnitude of spring can be regulated, thus control the size of spring force；First spring adjusting nut (3a), the second bullet
The use of spring adjusting nut (3b) may also function as locking, fastening effect；Housing (14) and T-shaped screw rod (2) junction are by first
Screw rod clamp nut (3d), the second screw rod clamp nut (3e) fastening；
Trigger mechanism include the first block arc (6a), the second block arc (6b), the first bolt (7a), the second bolt (7b),
3rd bolt (7c), split pin (8) and connection sheet (9), the upper end of the first block arc (6a) and the second block arc (6b) is equal
It is provided with snap ring；First block arc (6a) is provided with through hole, and described through hole is used for connecting split pin (8), the first block arc
(6a) He the second block arc (6b) is positioned opposite, constitutes similar circular ring structure, the first block arc (6a), perforated baffle (12)
Being connected by the first bolt (7a) with housing (14), the second block arc 6 b, perforated baffle (12) and housing (14) pass through second
Bolt (7b) connects；
Housing (14) bottom arranges cultivation cylinder 19；Be evenly arranged on housing (14) several syringes install passage (15b) and
Drainage channel (16b)；
Syringe (11a) one end sequentially passes through perforated baffle (12), housing (14), stretches in cultivation cylinder (19)；
Cable (1) one end connects the split pin (8) on the first block arc (6a)；
Silica gel pad (13) is installed on perforated baffle (12) top, by the position of regulation silica gel pad clamp nut (3c), makes
Silica gel pad (13) is pressed on perforated baffle (12), blocks the osculum (16a) on perforated baffle (12) and housing (14)
Drainage channel (16b)；When culture apparatus inserts in deposit, current can be upwardly through the drainage channel on housing (14)
(16b) osculum (16a) and on perforated baffle (12) is discharged；When cultivation completes, culture apparatus is taken out from seabed rear silicon
Glue gasket (13) may also function as preventing extraneous sea water from entering deposit and cultivates the effect of cylinder (19).
Device the most according to claim 1, it is characterised in that when leading on the first block arc (6a) is inserted in split pin (8)
Kong Shi, in the upper screwed hole arranged of the 3rd bolt (7c) precession the second block arc (6b), the 3rd bolt (7c) one end is by even
Contact pin (9) connects split pin (8), so that first snap ring (6c) of the first block arc (6a) and the second block arc (6b)
Second snap ring (6d) blocks lower baffle plate (10) and is allowed to can not continue down；When split pin (8) is not installed, the first block arc
(6a) freely rotatable by the first bolt (7a).
Device the most according to claim 1 a, it is characterised in that knot is made a call in cable (1) upper end, facilitates submersible machinery
The supporting operation of hands.
Device the most according to claim 3, it is characterised in that during use cable (1) and split pin (8) trigger device move
Making, when at the knot of deep seafloor submersible mechanical hand pull-up cable (1) upper end, split pin (8) will be taken advantage of a situation and be pulled out, make arc
Shape baffle plate (6a) rotates around bolt (7a) and slips off, and lower baffle plate (10), under the active force of spring (5), is pressed downward against syringe (11a)
Push rod, push rod moves and is injected in the deposit cultivated along cylinder (19) along syringe needle (11b) culture fluid that pours in advance.
Device the most according to claim 1, it is characterised in that cultivate cylinder (19) and select different size according to actual needs
Transparent organic glass cylinder, can be bonded in the lower end of housing (14), and be fixed by stainless steel lathe dog by adhesive waterproof tape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610249655.1A CN105861277B (en) | 2016-04-21 | 2016-04-21 | Underwater injecting type deposit Culture in situ device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610249655.1A CN105861277B (en) | 2016-04-21 | 2016-04-21 | Underwater injecting type deposit Culture in situ device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105861277A true CN105861277A (en) | 2016-08-17 |
| CN105861277B CN105861277B (en) | 2017-12-15 |
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| CN201610249655.1A Expired - Fee Related CN105861277B (en) | 2016-04-21 | 2016-04-21 | Underwater injecting type deposit Culture in situ device |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106893665A (en) * | 2015-11-19 | 2017-06-27 | 美天施生物科技有限责任公司 | From the cellifugal method and apparatus of biological tissue point |
| CN111084176A (en) * | 2019-12-31 | 2020-05-01 | 青岛罗博飞海洋技术有限公司 | Starfish carrying injector of underwater robot |
| CN112189615A (en) * | 2020-10-20 | 2021-01-08 | 中国科学院海洋研究所 | ROV-based deep sea in-situ large-scale organism stress device and use method thereof |
| CN112616757A (en) * | 2020-12-25 | 2021-04-09 | 中国科学院海洋研究所 | ROV-based deep sea in-situ large-scale organism culture device and use method thereof |
| WO2023072309A1 (en) * | 2021-12-28 | 2023-05-04 | 南方海洋科学与工程广东省实验室(广州) | Intelligent solution injection device for deep-sea sediment microorganism culture experiment |
| CN116413083A (en) * | 2023-06-12 | 2023-07-11 | 中国科学院深海科学与工程研究所 | Sediment in-situ culture and sampling device and working method |
| CN116429515A (en) * | 2023-06-12 | 2023-07-14 | 中国科学院深海科学与工程研究所 | Deep sea sediment pore water in-situ culture device and working method thereof |
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