CN106018720A - In-situ cultivation system and application method thereof - Google Patents
In-situ cultivation system and application method thereof Download PDFInfo
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- CN106018720A CN106018720A CN201610335478.9A CN201610335478A CN106018720A CN 106018720 A CN106018720 A CN 106018720A CN 201610335478 A CN201610335478 A CN 201610335478A CN 106018720 A CN106018720 A CN 106018720A
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Abstract
The invention provides an in-situ cultivation system and an application method thereof. The in-situ cultivation system comprises a mechanical structural frame and an electronic-control processing module. The mechanical structural frame comprises a cultivation vessel device, a liquid sample collection device and a lifting device; the electronic-control processing module comprises a power supply module, a processor module, a direct-current motor circuit module and a stepping motor circuit module. The liquid sample collection device comprises an injection device, a sampling device, a console, a measurement sensor, a control side panel, a stepping motor, a driving belt, a rolling bar, movable bars and the like. The in-situ cultivation system is applied to seabed water oxygen consumption process, is integrated with the electronic-control module, a motor, a vessel, a seawater measurement sensor and other mechanical modules, and descends to the seabed by gravity; a closed space is formed by the vessel and the seabed sediment interface; the measurement sensor in the vessel measures data of dissolved oxygen and environmental parameters continuously, and meanwhile, the measurement sensor arranged out of the vessel measures data of external water dissolved oxygen and environmental parameter continuously.
Description
Technical field
The present invention relates to water body Detection Techniques field, seabed, particularly relate to the Culture in situ system in a kind of oxygen process and use thereof
Method.
Background technology
The rapid economic development of Yangtze river basin recent decades, all kinds of nutrient concentrations making the Changjiang river carry rise tens times so that
The Eutrophic Extent of MATTER IN CHANGJIANG ESTUARY is more and more higher.Body eutrophication changes the structure of this marine site productivity, causes red tide
Frequent and large-area outburst, then causes the normalization of bottom water hypoxia phenomenon in summer to occur, causes China's East Sea fishery
Threaten, therefore monitoring, research and the Present assessment of seabed, entrance of Changjiang River water hypoxia process is had great importance.Entrance of Changjiang River
Existing many decades is carried out in seasonal big station, face measurements of dissolved oxygen, is to understand the important foundation observation of instantaneous anaerobic condition in territory, extra large
The Continuous Observation of bed base and buoy system presents the complete procedure that anoxia occurs, develops and wither away, and makes our understanding to anoxia
More comprehensively with deep.Bottom Dissolved Oxygen in Water is consumed by organic matter decomposition and can not get the most supplementary machine being anoxia and occurring
Reason, the observation such as station, big face, preventing seabed base and buoy is the denseest under various environmental factors comprehensive functions of dissolved oxygen in water body
Degree, it is organic matter decomposition oxygen consumption and the interaction result of vertical mixing oxygenating, if oxygen consumption under the conditions of varying environment can be obtained
With oxygenating process and speed, that just can grasp and predict degree and the change of water hypoxia, and this is to MATTER IN CHANGJIANG ESTUARY ecosystem
Study significant.The basis that China is studied due to ocean observation technology is weak, starting is late, consumes for seabed water body organic matter
The observation of oxygen process is also only limitted to laboratory simulation culture experiment, lacks the platform carrying out in-situ batch culture experiment.Although simulation experiment
Keep the environment identical with scene as far as possible, including temperature, salinity and light, but be intended to the natural environment that exact reproduction is on-the-spot,
It is nearly impossible, so the original position field observation technical research of oxygen process seems urgent and meaningful.
Summary of the invention
The technical problem to be solved in the present invention is: overcome the deficiencies in the prior art, it is provided that one can be placed directly in seabed, in sea
The water body oxygen process Culture in situ system used under end biology nature living environment and using method thereof.
To achieve the above object, present invention employs following technical scheme:
The present invention provides a kind of Culture in situ system, including frame for movement framework and automatically controlled processing module, described frame for movement framework
Including culture vessel device, sample liquid harvester and lowering or hoisting gear, described automatically controlled processing module includes power module, processor die
Block, direct current generator circuit module and motor circuit module, described sample liquid harvester include injection device, sampling apparatus,
Control station, measurement sensor, control side plate, motor, transmission band, rolling rod, motion bar, localizer and coil tension spring
Ⅱ。
Wherein: described injection device is the organic solution for injecting preset concentration value in container；Described culture vessel device
Including the container for gathering sample liquid, described lowering or hoisting gear is arranged on control station, and described container is fixedly mounted on control station lower end
Face, described lowering or hoisting gear can drive container to move up and down；Described automatically controlled processing module is fixedly installed on lowering or hoisting gear,
Motor circuit module is connected with motor；Direct current generator circuit module and measurement sensor are connected with processor module, place
Reason device module, direct current generator circuit module are connected with power module respectively；Described measurement sensor is fixedly mounted on control station, institute
Stating sampling apparatus to be connected with container, sampling apparatus is for carrying out sampling processing to the sample liquid gathered in container.
Rolling rod is in transmission connection with motor by transmission band, and described rolling rod is provided with runner, the top of motion bar and location
Device connects, and the bottom of motion bar can offset with runner, and described localizer extends through its opposite side from the one side controlling side plate
Face, runner can rotate along with rolling rod, and motion bar is connected with controlling side plate so that controls side plate and can support work
Prizing of lever, prizes localizer by motion bar and localizer can be stretched on the direction be perpendicular to control side plate
Mobile；Described sampling apparatus is connected by sampling cavity and the sampling body of rod and forms, and sampling cavity is connected with container by conduit；
Described sampling cavity is fixed on control side plate, and sampling body of rod top can slide up and down along wall in sampling cavity body, from
And realize sampling apparatus pull sample liquid or squeeze push away reagent；Described sampling body of rod bottom is connected with coil tension spring II, coil tension spring
The bottom of II is fixed on control side plate, and it is identical that the telescopic direction of coil tension spring II moves up and down direction with the sampling body of rod；Described fixed
The notch that can be used in the buckle sampling body of rod is offered on one end of position device；When in the sampling notch that is buckled on localizer of the body of rod,
Now coil tension spring II is in extended state, drives rolling rod to rotate by starting motor, drives turning on rolling rod simultaneously
Wheel rotates, and the runner in rotation process can stir the motion bar offseted with this runner, and motion bar prizes localizer and makes to determine
Position device can carry out telescopic moving on the direction be perpendicular to control side plate, so that the sampling body of rod is separated from the groove of localizer
Mouthful, the stretching action down-sampling body of rod at coil tension spring II moves downward, it is achieved pull sample liquid in the sampling calm device of cavity.
Preferably: described sample liquid harvester also includes that stirring motor, described culture vessel device also include paddle
Wheel, direct current generator circuit module is connected with stirring motor, and direct current generator circuit module is connected with power module respectively；Described stirring
Motor is fixedly mounted on control station, and agitator arm is arranged in container, and agitator arm is electrically connected with stirring motor, stirring electricity
Machine is stirred motion for controlling agitator arm to the sample liquid gathered in container.
Preferably: described culture vessel device also includes the base matched with container, base is provided with connecting rod at the bottom of
The screwed hole that the external screw thread II that end is provided with matches, is additionally provided with the first base plate through holes that several spacing are evenly distributed on base,
These first base plate through holes can reduce sea water drag effect in whole device or system transfer sea water process of the test effectively, greatly
The earth improves lowering velocity.
Preferably: described control station includes controlling base plate, ring washer and controlroof, control base plate and pass through air ring
Enclose be connected fixing with controlroof；Described ring washer is arranged between control base plate and controlroof mainly to play and is tightly connected
Effect；The top and bottom of described container are uncovered structure, and the upper end of container is fixedly mounted on control base plate, under container
The second base plate through holes that end sets in can extending through base.
Preferably: the first through hole that stirring motor sets in extending through controlroof also downwardly extends and is arranged in container
Agitator arm be in transmission connection, be provided with first annular groove in the first through hole upper end of controlroof, embedded at first annular groove
It is equipped with the ring washer for being tightly connected effect so that stirring motor is sealedly connected on controlroof by ring washer.
Preferably: the second through hole that described measurement sensor sets in extending through controlroof also extends downwardly into container
In, to measure and be connected and sealed by fastening clamp ring and gasket ring block between sensor and controlroof, fastening clamp ring is arranged on gasket ring
Block upper end, if being equipped with arterial highway elastic washer arranged in parallel I on the gasket ring through-hole wall of gasket ring block, and upper at gasket ring block
Elastic washer II it is respectively equipped with, the axle center of these elastic washers II and the position, axle center of gasket ring through hole in gasket ring block on surface and lower surface
On same axial line；The equal semicircular in shape of cross section of elastic washer I and elastic washer II or half elliptic.
Wherein: elastic washer II mainly realizes the connection sealing effectiveness between controlroof, elastic washer I mainly realizes
And the connection sealing effectiveness measured between sensor.
Preferably: described lowering or hoisting gear includes support, connecting rod, sleeve pipe I, sleeve pipe II and coil tension spring III, described
Frame is fixedly mounted on the control base plate of control station；Collar and lower collar it is provided with, in upper collar and lower collar in described support
Being respectively equipped with through hole and lower through-hole, described upper collar is connected with lower collar by sleeve pipe II, and set in upper through hole and sleeve pipe II
It is interconnected between sleeve pipe II through hole and the lower through-hole that set between sleeve pipe II through hole and in sleeve pipe II；Described connecting rod is from top to bottom
Sequentially pass through sleeve pipe I through hole by setting in sleeve pipe I, upper through hole, sleeve pipe II through hole, lower through-hole and coil tension spring III, even
The top of bar and bottom are respectively equipped with external screw thread I, external screw thread II, wherein: the external screw thread II of connecting rod bottom can be with whorl of base
Connect and be embedded in base, and coil tension spring III top with control base plate offsets, coil tension spring III bottom offsets with base；?
The external screw thread I on connecting rod top is provided with the nut I matched.
Specifically, lowering or hoisting gear is being moved down in operating process, can be by being rotated down twisting nut I, at nut I
After offseting with the top of sleeve pipe I, along with nut I continues to rotate twisting so that connecting rod protrudes upward, and simultaneous retractable spring III is subject to
Compressing after extruding and gradually shorten, the most now lowering or hoisting gear moves gradually downward, simultaneously lowering or hoisting gear drive container move down and to
Lower extension extends through the second base plate through holes on base.
Preferably: being provided with through hole II on base plate controlling, connecting rod extends through this through hole II, the lower collar on support with
Control base plate to offset；Ensure that lowering or hoisting gear and control to remain steady status between base plate so that connecting rod bottom with base
After threaded, drive container to move down by being moved down by lowering or hoisting gear so that the lower end of container extends through the end simultaneously
The second base plate through holes set in Zuo, so that container collection form the sample liquid of steady statue.
Preferably: in localizer, be arranged with coil tension spring I, one end of this coil tension spring I and localizer A end phase
Supporting, the notch that the other end of coil tension spring I is provided with localizer B end offsets；Telescopic process is carried out at coil tension spring I
In so that mutually draw close between localizer A end and localizer B end or be located remotely from each other；Motion bar is provided with controlling side plate
Support member be flexibly connected, this support member is to realize the support zone during motion bar prizes, when the runner on rolling rod and work
When lever one end offsets, the motion bar other end is connected with localizer, and now the thief rod body of sampling apparatus can stably be buckled in
In the notch of localizer B end；The runner on rolling rod is driven to rotate starting motor so that motion bar one end takes off
Leaving runner, the motion bar other end prizes localizer immediately, under coil tension spring I acts on so that localizer B end is toward location
Device A end is flexible to be drawn close, so that the sampling body of rod of sampling apparatus departs from the notch of localizer B end, the sampling of sampling apparatus
The body of rod moves down under coil tension spring II acts on, it is achieved the sample liquid that sampling cavity extraction is enough.
The present invention also provides for the using method of a kind of Culture in situ system, specifically includes following operating procedure:
First passing through lowering or hoisting gear drives container to move down so that container extends through the second base plate through holes of base, then by whole
Individual Culture in situ system is transferred in predetermined maritime area, until base reach bottom surface, predetermined maritime area stable after, start and measure sensor and stir
Mixing motor, stirring motor drives the sample liquid being arranged in container, simultaneously by the parameter of sample liquid in measurement sensor detection container；
Then starting motor drives rolling rod to rotate, and drives the runner on rolling rod to rotate, in rotation process simultaneously
The motion bar offseted with this runner stirred by runner, and motion bar prizes localizer and makes localizer along the direction being perpendicular to control side plate
On carry out telescopic moving, so that the sampling body of rod is separated from the notch of localizer, at the stretching action down-sampling of coil tension spring II
The body of rod moves downward, it is achieved pull sample liquid in the sampling calm device of cavity.
Preferably: described measurement sensor is to use RBR-XR420 multi-parameter water quality instrument, is configured with temperature, salt
Degree, the degree of depth, dissolved oxygen and 5 parameters of turbidity.
Preferably: described sampling apparatus includes several syringes, wherein: sampling cavity is the injecting cavity of syringe
Body, the sampling body of rod is the push handle of syringe.
Compared with prior art, the invention has the beneficial effects as follows:
Due to the fact that and use above-mentioned technical scheme, Culture in situ system be applied to seabed water body oxygen process a set of automatically
Changing observation and culture device, integral electronic control module, motor, container, sea water measure sensor and other frame for movement modules
Being integrated, drop to seabed by self gravitation, container forms confined space with bottom sediment interface, is positioned at the survey of container
Quantity sensor measures dissolved oxygen and ambient parameter data continuously, and the measurement sensor being positioned at outside container measures outside body of water simultaneously continuously
Dissolved oxygen and ambient parameter data；Sample liquid harvester injects having of preset concentration value by above-mentioned mechanical structure device in container
Machine matter solution (can also select to be not injected into any solution, the simple oxygen process cultivating water body itself), after fixed time intervals
Sample liquid harvester extracts the sample liquid of fixed volume in container, reclaims the parameter such as post analysis nutritive salt and organic carbon in order to last.Former
Position culture experiment can be from several hours to several days, and inside and outside culture vessel, Dissolved Oxygen in Water all can have a time-varying process,
In conjunction with other ambient parameter datas thus understand the oxygen process of water body under site environment.
Accompanying drawing explanation
Fig. 1 is the overall structure schematic diagram of a kind of Culture in situ system in the present invention.
Fig. 2 is the detonation configuration schematic diagram of Fig. 1.
Fig. 3 is the detonation configuration schematic diagram of lowering or hoisting gear in the present invention.
Fig. 4 is one of one side mounting structure schematic diagram controlling side in the present invention.
Fig. 5 is the two of the one side mounting structure schematic diagram controlling side in the present invention.
Fig. 6 is the another side mounting structure schematic diagram controlling side in the present invention.
Fig. 7 is the fit structure schematic diagram between the sampling body of rod and notch in invention.
Fig. 8 is the detonation configuration schematic diagram of control station in the present invention.
Fig. 9 is to measure the fit structure schematic diagram between sensor and control station in the present invention.
Figure 10 is the structural representation of gasket ring block in the present invention.
Reference:
Automatically controlled processing module 1, lowering or hoisting gear 2, support 201, upper collar 201A, lower collar 201B, connecting rod 202, outer spiral shell
Stricture of vagina I 202A, external screw thread II 202B, sleeve pipe I 203, sleeve pipe II 204, coil tension spring III 205, nut 206A, nut
206B；
Injection sampling module 3, control side plate 301, motor 302, transmission band 303, rolling rod 304, runner 305,
Motion bar 306, localizer 307, notch 3071, coil tension spring I 308, support member 309, cavity 310 of sampling, thief rod
Body 311, coil tension spring II 312, conduit 313；
Control base plate 401, through hole I 401A, annular groove 401B, through hole II 401C, stirring motor 402, measure sensing
Device 403, ring washer 404, controlroof 405, first annular groove 406, the first through hole 407, the second through hole 408,
Fastening clamp ring 409, gasket ring block 410, elastic washer I 410B, elastic washer II 410A, gasket ring through hole 410C；
Agitator arm 501, container 502, base 503, the first base plate through holes 504, the second base plate through holes 505.
Detailed description of the invention
Below in conjunction with the accompanying drawings the detailed description of the invention of the present invention is made a detailed explanation.
Embodiment:
As shown in Fig. 1～10, the present invention provides a kind of Culture in situ system and the specific embodiment of using method thereof, such as Fig. 1～2
Shown in, the Culture in situ system in the present invention includes frame for movement framework and automatically controlled processing module 1, described frame for movement framework bag
Including culture vessel device, sample liquid harvester and lowering or hoisting gear, described automatically controlled processing module 1 includes power module, processor die
Block, direct current generator circuit module and motor circuit module, described sample liquid harvester includes injection device, sampling cartridge
Put, control station, measurement sensor 403, control side plate 301, motor 302, transmission band 303, rolling rod 304, live
Lever 306, localizer 307 and coil tension spring II 312.
Wherein: described injection device is the organic solution for injecting preset concentration value in container；Specifically, in the present invention
Injection device is to use to be arranged on to control on side plate 301 and for the injection injecting preset concentration value organic matter solution in container
Device.
Described culture vessel device includes the container 502 for gathering sample liquid, and described lowering or hoisting gear is arranged on control station, described
Container 502 is fixedly mounted on control station lower surface, and described lowering or hoisting gear can drive container 502 to move up and down；Described electricity
Control processing module 1 is fixedly installed on lowering or hoisting gear 2, and motor circuit module is connected with motor 302；Direct current generator
Circuit module and measure sensor 403 be connected with processor module, processor module, direct current generator circuit module respectively with power supply
Module connects；Described measurement sensor 403 is fixedly mounted on control station, and described sampling apparatus is connected with container 502, sampling
Device is for carrying out sampling processing to the sample liquid gathered in container 502；Described sampling processing is mainly the sample liquid in container 502
It is drawn in sampling apparatus, in order to the needs of follow-up work carry out backing up and leave enough sample liquid.
As shown in Figure 6, described rolling rod 304 is in transmission connection with motor 301 by transmission band 303, described rolling rod
304 are provided with runner 305, and the top of motion bar 306 is connected with localizer 307, and the bottom of motion bar 306 can be with runner
305 offset, and described localizer 307 extends through its another side from the one side controlling side plate 301, and runner 305 can be along with
Rolling rod 304 rotates, and motion bar 306 is connected with controlling side plate 301 so that controls side plate 301 and can support work
Prizing of lever 306, by motion bar 306 prize localizer 307 make localizer 307 can along be perpendicular to control side plate
Telescopic moving is carried out on the direction of 301.Described sampling apparatus is connected by sampling cavity 310 and the sampling body of rod 311 and forms, and adopts
Sample cavity 310 is connected with container 502 by conduit 313；Described sampling cavity 310 is fixed on control side plate 301, adopts
The sample body of rod 311 top can slide up and down along sampling cavity 310 internal face, thus realizes the pull sample liquid of sampling apparatus
Or squeeze and push away reagent；The described sampling body of rod 311 bottom is connected with coil tension spring II 312, and the bottom of coil tension spring II 312 is fixed
Controlling on side plate 301, it is identical that the telescopic direction of coil tension spring II 312 moves up and down direction with the sampling body of rod 311；Described fixed
The notch 3071 that can be used in the buckle sampling body of rod 311 is offered on one end of position device 307；When the sampling body of rod 311 is buckled in
In notch 3071 on localizer 307, now coil tension spring II 312 is in extended state, by starting motor 302
Drive rolling rod 304 to rotate, drive the runner 305 on rolling rod 304 to rotate, the runner 305 in rotation process simultaneously
Can stir the motion bar 306 offseted with this runner 305, motion bar 306 prizes localizer 307 and makes the localizer 307 can
Telescopic moving is carried out, so that the sampling body of rod 311 is separated from localizer 307 on the direction be perpendicular to control side plate 301
Notch 3071, the stretching action down-sampling body of rod 311 at coil tension spring II 312 moves downward, it is achieved sampling cavity
310 in container 502 pull sample liquid.
Specifically: sampling apparatus is mainly made up of several syringes, wherein side by side in embodiments of the present invention: syringe is
Being connected by injection cavity and push handle and form, described injection cavity is fixed on control side plate 301, and push handle top can be along injection
Cavity inner wall face slides up and down, thus realize sampling apparatus pull sample liquid or squeeze push away reagent；Described push handle bottom with stretch
Contracting spring II 312 connects, and the bottom of coil tension spring II 312 is fixed on control side plate 301, stretching of coil tension spring II 312
It is identical that direction moves up and down direction with push handle.Offer, on one end of described localizer 307, the notch that can be used in buckle push handle
3071；When, in the notch 3071 that pushing handle card is buckled on localizer 307, now coil tension spring II 312 is in extended state, logical
Crossing startup motor 302 drives rolling rod 304 to rotate, and drives the runner 305 on rolling rod 304 to rotate simultaneously, turns
Runner 305 during Dong can stir the motion bar 306 offseted with this runner 305, and motion bar 306 prizes localizer 307
Make localizer 307 can carry out telescopic moving, so that push handle is separated from the direction be perpendicular to control side plate 301
The notch 3071 of localizer 307, under the stretching action of coil tension spring II 312, push handle moves downward, it is achieved injection cavity
In container 502, pull sample liquid realizes sampling.
Described sample liquid harvester also includes that stirring motor 402, described culture vessel device also include agitator arm 501, direct current
Motor circuit module is connected with stirring motor 402, and direct current generator circuit module is connected with power module respectively；Described stirring motor
402 are fixedly mounted on control station, and agitator arm 501 is arranged in container 502, agitator arm 501 and stirring motor 402
Electrical connection, stirring motor 402 is stirred fortune for controlling agitator arm 501 to the sample liquid gathered in container 502
Dynamic.
As it is shown in figure 1, described culture vessel device also includes the base 503 matched with container 502, base 503 sets
The screwed hole having external screw thread II 202B being provided with connecting rod 202 bottom to match, is additionally provided with several spacing on base 503
The first evenly distributed base plate through holes 504, these first base plate through holes 504 transfer sea water process of the test at whole device or system
In can effectively reduce sea water drag effect, greatly improve lowering velocity.
As illustrated in figures 1 and 8, described control station includes controlling base plate 401, ring washer 404 and controlroof 405, control
Base plate 401 processed is connected by ring washer 404 is fixing with controlroof 405；Described ring washer 404 is arranged at control base plate
Mainly play between 401 and controlroof 405 and be tightly connected effect；The top and bottom of described container 502 are uncovered knot
Structure, the upper end of container 502 is fixedly mounted on control base plate 401, and the lower end of container 502 can extend through in base 503
If the second base plate through holes 505.
The first through hole 407 that stirring motor 402 sets in extending through controlroof 405 also downwardly extends and is arranged on container 502
Interior agitator arm 501 is in transmission connection, and is provided with first annular groove 406 in the first through hole 407 upper end of controlroof 405,
The ring washer for being tightly connected effect it is equipped with so that stirring motor 402 passes through air ring in first annular groove 406
Circle is sealedly connected on controlroof 405.
As shown in Fig. 9～10, the second through hole 408 that described measurement sensor 403 sets in extending through controlroof 405 and to
Under extend in container 502, measure between sensor 403 and controlroof 405 by fastening clamp ring 409 and gasket ring block 410
Being connected and sealed, fastening clamp ring 409 is arranged on gasket ring block 410 upper end, at the gasket ring through hole 410C inwall of gasket ring block 410
It is equipped with arterial highway elastic washer arranged in parallel I 410B on if, and sets respectively in the upper and lower surface of gasket ring block 410
Flexible packing ring II 410A, the axle center of these elastic washers II 410A and the position, axle center of gasket ring through hole 410C in gasket ring block 410
On same axial line；The equal semicircular in shape of cross section of elastic washer I 410B and elastic washer II 410A or half elliptic.
Wherein: elastic washer II 410A mainly realizes the connection sealing effectiveness between controlroof 405, elastic washer
The connection sealing effectiveness that I 410B mainly realizes and measures between sensor 403.
As it is shown on figure 3, described lowering or hoisting gear 2 includes support 201, connecting rod 202, sleeve pipe I 203, sleeve pipe II 204 and stretches
Spring III 205, described support 201 is fixedly mounted on the control base plate 401 of control station；It is provided with top in described support 201
Circle 201A and lower collar 201B, is respectively equipped with through hole and lower through-hole in upper collar 201A and lower collar 201B, described
Upper collar 201A is connected with lower collar 201B by sleeve pipe II 204, and sleeve pipe II through hole set in upper through hole and sleeve pipe II 204
Between and sleeve pipe II 204 in be interconnected between sleeve pipe II through hole and the lower through-hole that set；Described connecting rod 202 depends on from top to bottom
Secondary extend through sleeve pipe I 203 in sleeve pipe I through hole, upper through hole, sleeve pipe II through hole, lower through-hole and the coil tension spring that set
III 205, it is respectively equipped with external screw thread I 202A, external screw thread II 202B on the top of connecting rod 202 and bottom, wherein: connecting rod 202
External screw thread II 202B of bottom can be threadeded with base 503 and be embedded in base 503, sets on external screw thread II 202B
There is nut II 206B of coupling；Coil tension spring III 205 top with control base plate 401 offset, coil tension spring III 205 bottom and the end
Seat 503 offsets；External screw thread I 202A on connecting rod 202 top is provided with nut I 206A matched.
Specifically, lowering or hoisting gear 2 is being moved down in operating process, nut I 206A can twisted by being rotated down,
After the top of nut I 206A Yu sleeve pipe I 203 offsets, along with nut I 206A continues to rotate twisting so that connecting rod 202
Protruding upward, simultaneous retractable spring III 205 compresses after being squeezed and gradually shortens, and the most now lowering or hoisting gear 2 moves gradually downward,
Lowering or hoisting gear 2 drives container 502 to move down and extend downwardly through by the second base plate through holes 505 on base 503 simultaneously.
As shown in Figure 8, being provided with through hole II 401C on control base plate 401, connecting rod 202 extends through this through hole II 401C,
Lower collar 201B on support 201 offsets with controlling base plate 401；Ensure that lowering or hoisting gear 2 and control to begin between base plate 401
Keep steady status eventually so that connecting rod 202 bottom is after threadeding with base 503, by being moved down by lowering or hoisting gear 2
Drive container 502 to move down so that the second base plate through holes that the lower end of container 502 sets in extending through base 503 simultaneously
505, so that container 502 gathers and is formed the sample liquid of steady statue.
As also shown in e.g. figs. 4-7, being arranged with coil tension spring I 308 in localizer 307, one end of this coil tension spring I 308 is with fixed
Position device 307A end offsets, and the notch 3071 that the other end of coil tension spring I 308 is provided with localizer 307B end offsets；?
Coil tension spring I 308 carries out in telescopic process so that mutually draw close or phase between localizer 307A end with localizer 307B end
Mutually away from；The support member 309 that motion bar 306 is provided with control side plate 301 is flexibly connected, and this support member 309 is to realize living
Lever 306 prize during support zone, when the runner 305 on rolling rod 304 offsets with motion bar 306 one end, live
Lever 306 other end is connected with localizer 307, and now the sampling body of rod 311 on sampling apparatus can stably be buckled in location
In the notch 3071 of device 307B end；The runner 305 on rolling rod 304 is driven to rotate starting motor 302,
Making motion bar 306 one end be separated from runner 305, motion bar 306 other end prizes localizer 307, at coil tension spring immediately
Under I 308 effects so that localizer 307B end is drawn close toward localizer 307A end is flexible, so that the thief rod of sampling apparatus
Body 311 departs from the notch 3071 of localizer 307B end, and the sampling body of rod 311 of sampling apparatus is under coil tension spring II 312 acts on
Move down, it is achieved sampling cavity 310 extracts enough sample liquid.
The embodiment of the present invention also provides for the using method of a kind of Culture in situ system, specifically includes following operating procedure:
First passing through lowering or hoisting gear 2 drives container 502 to move down so that container 502 extends through the second base of base 503
Through hole 505, then transfers in predetermined maritime area by whole Culture in situ system, treats that base 503 reaches bottom surface, predetermined maritime area stable
After, starting and measure sensor 403 and stirring motor 402, stirring motor 402 drives the sample liquid being arranged in container 502, with
Time detect the parameter of sample liquid in container 502 by measuring sensor 403；
Then starting motor 302 drives rolling rod 304 to rotate, and drives the runner 305 on rolling rod 304 to carry out turning simultaneously
Dynamic, the motion bar 306 offseted with this runner 305 stirred by the runner 305 in rotation process, and motion bar 306 prizes localizer
307 make localizer 307 carry out telescopic moving on the direction be perpendicular to control side plate 301, so that the sampling body of rod
311 notches 3071 being separated from localizer 307, the stretching action down-sampling body of rod 311 at coil tension spring II 312 is carried out downwards
Motion, it is achieved sampling cavity 310 pull sample liquid in container 502.
Wherein: described measurement sensor 403 is to use RBR-XR420 multi-parameter water quality instrument, temperature, salinity, deep it is configured with
Degree, dissolved oxygen and 5 parameters of turbidity.
The ultimate principle of the present invention and principal character and advantages of the present invention have more than been shown and described.The technical staff of the industry should
This understanding, the present invention is not restricted to the described embodiments, and the simply explanation present invention's described in above-described embodiment and description is former
Reason, without departing from the spirit and scope of the present invention, the present invention also has various changes and modifications, these changes and improvements
Both fall within scope of the claimed invention.Claimed scope is by appending claims and equivalent circle thereof
Fixed.
Claims (10)
1. a Culture in situ system, it is characterised in that include frame for movement framework and automatically controlled processing module (1), described machine
Tool structural framing includes that culture vessel device, sample liquid harvester and lowering or hoisting gear, described automatically controlled processing module (1) include electricity
Source module, processor module, direct current generator circuit module and motor circuit module, described sample liquid harvester includes adopting
Sampling device, control station, measurement sensor (403), control side plate (301), motor (302), transmission band
(303), rolling rod (304), motion bar (306), localizer (307) and coil tension spring II (312), described training
Foster case includes the container (502) for gathering sample liquid, and described lowering or hoisting gear is arranged on control station, described container
(502) being fixedly mounted on control station lower surface, described lowering or hoisting gear can drive container (502) to move up and down；Described
Automatically controlled processing module (1) is fixedly installed on lowering or hoisting gear, and motor circuit module is connected with motor (302)；Directly
Stream motor circuit module and measurement sensor (403) are connected with processor module, processor module, direct current generator circuit module
It is connected with power module respectively；Described measurement sensor (403) is fixedly mounted on control station, described sampling apparatus and container
(502) being connected, the sample liquid that sampling apparatus gathers in container (502) carries out sampling processing；
Rolling rod (304) is in transmission connection with motor (301), on described rolling rod (304) by transmission band (303)
Being provided with runner (305), the top of motion bar (306) is connected with localizer (307), and the bottom of motion bar (306) can
Offseting with runner (305), described localizer (307) extends through its another side from the one side controlling side plate (301),
Runner (305) can rotate along with rolling rod (304), and motion bar (306) connects with controlling side plate (301)
Connect so that control side plate (301) and prizing of motion bar (306) can be supported, prize localizer by motion bar (306)
(307) localizer (307) is made telescopic moving can be carried out on the direction be perpendicular to control side plate (301)；
Described sampling apparatus is connected by sampling cavity (310) and the sampling body of rod (311) and forms, and sampling cavity (310) is led to
Cross conduit (313) to be connected with container (502)；Described sampling cavity (310) is fixed in control side plate (301), adopts
The sample body of rod (311) top can slide up and down along sampling cavity (310) internal face, thus realizes taking out of sampling apparatus
Draw sample liquid or squeeze push away reagent；The described sampling body of rod (311) bottom is connected with coil tension spring II (312), coil tension spring II
(312) bottom is fixed in control side plate (301), the telescopic direction of coil tension spring II (312) and the sampling body of rod
(311) direction is moved up and down identical；Offer on one end of described localizer (307) and can be used in the buckle sampling body of rod
(311) notch (3071)；When in the sampling body of rod (311) notch (3071) that is buckled on localizer (307),
Now coil tension spring II (312) is in extended state, drives rolling rod (304) to turn by starting motor (302)
Dynamic, drive the runner (305) on rolling rod (304) to rotate simultaneously, the runner (305) in rotation process can be dialled
The dynamic motion bar (306) offseted with this runner (305), motion bar (306) prizes localizer (307) and makes localizer
(307) telescopic moving can be carried out on the direction be perpendicular to control side plate (301), so that the sampling body of rod
(311) notch (3071) of localizer (307) it is separated from, at the stretching action down-sampling bar of coil tension spring II (312)
Body (311) moves downward, it is achieved sampling cavity (310) pull sample liquid in container (502).
2. according to a kind of Culture in situ system described in claim 1, it is characterised in that described sample liquid harvester also wraps
Including stirring motor (402), described culture vessel device also includes agitator arm (501), direct current generator circuit module and stirring
Motor (402) connects, and direct current generator circuit module is connected with power module respectively；Described stirring motor (402) fixedly mounts
On control station, agitator arm (501) is arranged in container (502), agitator arm (501) and stirring motor (402)
Electrical connection, stirring motor (402) is used for controlling agitator arm (501) and enters the sample liquid gathered in container (502)
Row stirring movement.
3. according to a kind of Culture in situ system described in claim 1 or 2, it is characterised in that described culture vessel device
Also include the base (503) matched with container (502), base (503) is provided with and is provided with connecting rod (202) bottom
The screwed hole that matches of external screw thread II (202B), be additionally provided with on base (503) that several spacing are evenly distributed first
Base plate through holes (504).
4. according to a kind of Culture in situ system described in claim 3, it is characterised in that described control station includes controlling the end
Plate (401), ring washer (404) and controlroof (405), control base plate (401) by ring washer (404)
Fix with controlroof (405) and be connected；The top and bottom of described container (502) are uncovered structure, container (502)
Upper end be fixedly mounted in control base plate (401), the lower end of container (502) sets in can extending through base (503)
The second base plate through holes (505).
5. according to a kind of Culture in situ system described in claim 4, it is characterised in that stirring motor (402) runs through logical
The first through hole (407) of setting in crossing controlroof (405) also downwardly extends and is arranged on the agitator arm in container (502)
(501) it is in transmission connection, is provided with first annular groove (406) in the first through hole (407) upper end of controlroof (405),
It is equipped with ring washer so that stirring motor (402) is sealedly connected on by ring washer in first annular groove (406)
On controlroof (405).
6. according to a kind of Culture in situ system described in claim 4, it is characterised in that described measurement sensor (403)
The second through hole (408) of setting in extending through controlroof (405) in extending downwardly into container (502), measures sensing
It is connected and sealed, tightly by fastening clamp ring (409) and gasket ring block (410) between device (403) and controlroof (405)
Gu clamp ring (409) is arranged on gasket ring block (410) upper end, ring on gasket ring through hole (410C) inwall of gasket ring block (410)
If being provided with arterial highway elastic washer arranged in parallel I (410B), and in the upper and lower surface of gasket ring block (410) respectively
It is provided with elastic washer II (410A), the axle center of these elastic washers II (410A) and gasket ring block (410) interior gasket ring through hole
(410C) axle center is positioned on same axial line；Elastic washer I (410B) and the cross section of elastic washer II (410A)
All semicircular in shape or half elliptics.
7. according to a kind of Culture in situ system described in claim 1, it is characterised in that described lowering or hoisting gear (2) includes
Support (201), connecting rod (202), sleeve pipe I (203), sleeve pipe II (204) and coil tension spring III (205), described
Support (201) is fixedly mounted on control station；Collar (201A) and lower collar it is provided with in described support (201)
(201B), in upper collar (201A) and lower collar (201B), upper through hole and lower through-hole, described upper collar it are respectively equipped with
(201A) it is connected with lower collar (201B) by sleeve pipe II (204), and the set set in upper through hole and sleeve pipe II (204)
It is interconnected between sleeve pipe II through hole and the lower through-hole that set between pipe II through hole and in sleeve pipe II (204)；
Described connecting rod (202) sequentially passes through sleeve pipe I through hole by setting in sleeve pipe I (203), upper through hole, set from top to bottom
Pipe II through hole, lower through-hole and coil tension spring III (205), be respectively equipped with external screw thread I on the top of connecting rod (202) and bottom
(202A), external screw thread II (202B), wherein: the external screw thread II (202B) of connecting rod (202) bottom can be with base
(503) threaded and in being embedded in base (503), and coil tension spring III (205) top and control base plate (401) phase
Support, coil tension spring III (205) bottom offsets with base (503)；External screw thread I (202A) on connecting rod (202) top
It is provided with the nut I (206A) matched.
8. according to a kind of Culture in situ system described in claim 7, it is characterised in that be provided with through hole II on control station
(401C), connecting rod (202) extends through this through hole II (401C), the lower collar (201B) on support (201) with
Control station offsets；Make connecting rod (202) bottom after threadeding with base (503), by lowering or hoisting gear is moved down
Drive container (502) to move down simultaneously so that the lower end of container (502) set in extending through base (503) second
Base plate through holes (505), so that container (502) gathers and is formed the sample liquid of steady statue.
A kind of Culture in situ system described in the most according to Claim 8, it is characterised in that sheathed in localizer (307)
Having coil tension spring I (308), one end of this coil tension spring I (308) offsets with localizer (307) A end, coil tension spring
The notch (3071) that the other end of I (308) and localizer (307) B end are provided with offsets；At coil tension spring I
(308) carry out in telescopic process so that mutually draw close between localizer (307) A end and localizer (307) B end or
It is located remotely from each other；The support member (309) that motion bar (306) is provided with control side plate (301) is flexibly connected, this support member
(309) be to realize the support zone during motion bar (306) prizes, when the runner (305) on rolling rod (304) with
When motion bar (306) one end offsets, motion bar (306) other end is connected with localizer (307), now sampling apparatus
The sampling body of rod (311) can stably be buckled in the notch (3071) of localizer (307) B end；Starting motor
(302) runner (305) on rolling rod (304) is driven to rotate so that motion bar (306) one end is separated from runner
(305), motion bar (306) other end prizes localizer (307) immediately, under coil tension spring I (308) acts on, makes
Obtain localizer (307) B end to draw close toward localizer (307) A end is flexible, so that the sampling body of rod of sampling apparatus
(311) departing from the notch (3071) of localizer (307) B end, the sampling body of rod (311) of sampling apparatus is at coil tension spring
Move down under II (312) effect, it is achieved enough sample liquid is extracted in sampling cavity (310).
10. using method based on a kind of Culture in situ system described in claim 9, it is characterised in that specifically include as follows
Operating procedure:
First passing through lowering or hoisting gear drives container (502) to move down so that container (502) extends through base (503)
Second base plate through holes (505), then transfers in predetermined maritime area by whole Culture in situ system, treats that base (503) reaches pre-
After bottom surface, territory, Dinghai is stable, starting and measure sensor (403) and stirring motor (402), stirring motor (402) drives and sets
Put the sample liquid in container (502), simultaneously by measuring the parameter of sensor (403) detection container (502) interior sample liquid；
Then starting motor (302) drives rolling rod (304) to rotate, and drives the runner on rolling rod (304) simultaneously
(305) rotating, the motion bar (306) offseted with this runner (305) stirred by the runner (305) in rotation process,
Motion bar (306) prize localizer (307) make localizer (307) along be perpendicular to control side plate (301) direction on
Carry out telescopic moving, so that the sampling body of rod (311) is separated from the notch (3071) of localizer (307), at flexible bullet
The stretching action down-sampling body of rod (311) of spring II (312) moves downward, it is achieved sampling cavity (310) is from container
(502) interior pull sample liquid.
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| CN201610335478.9A CN106018720B (en) | 2016-05-19 | 2016-05-19 | Culture in situ system and its application method |
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| CN201610335478.9A CN106018720B (en) | 2016-05-19 | 2016-05-19 | Culture in situ system and its application method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112130212A (en) * | 2020-08-21 | 2020-12-25 | 中国石油天然气集团有限公司 | Bearing body structure of marine electromagnetic node acquisition station |
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