CN106018720B - Culture in situ system and its application method - Google Patents
Culture in situ system and its application method Download PDFInfo
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- CN106018720B CN106018720B CN201610335478.9A CN201610335478A CN106018720B CN 106018720 B CN106018720 B CN 106018720B CN 201610335478 A CN201610335478 A CN 201610335478A CN 106018720 B CN106018720 B CN 106018720B
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Abstract
The present invention provides a kind of Culture in situ system and its application method, its Culture in situ system includes mechanical structure framework and automatically controlled processing module, the mechanical structure framework includes culture vessel device, sample liquid harvester and lowering or hoisting gear, the automatically controlled processing module includes power module, processor module, direct current generator circuit module and stepper motor circuit module, and the sample liquid harvester includes injection device, sampling apparatus, console, measurement sensor, control side plate, stepper motor, transmission belt, rolling rod, motion bar etc..The present invention is applied to seabed water body oxygen process, integrated electricity controlled module, motor, container, seawater measurement sensor and other mechanical modules are integrated, seabed is dropped to by self gravitation, container forms confined space with bottom sediment interface, measurement sensor in container continuously measures dissolved oxygen and ambient parameter data, and the measurement sensor outside container is simultaneously continuous to measure outside body of water dissolved oxygen and ambient parameter data.
Description
Technical field
The present invention relates to seabed water body detection technology field, more particularly to a kind of Culture in situ system in oxygen process and
Its application method.
Background technology
The rapid economic development of Yangtze river basin recent decades, all kinds of nutrient concentrations that the Changjiang river carries are made to rise tens
Times so that the Eutrophic Extent more and more higher of MATTER IN CHANGJIANG ESTUARY.Body eutrophication changes the structure of this marine site productivity,
Cause red tide frequently and the outburst of large area, then cause the normalization of summer bottom water hypoxia phenomenon to occur, it is eastern to China
Extra large fishery causes threat, thus monitoring, research and the Present assessment to entrance of Changjiang River seabed water hypoxia process have it is important
Meaning.Existing many decades are carried out in the big face station measurement of seasonality of entrance of Changjiang River dissolved oxygen, are the weights for understanding instantaneous anaerobic condition in domain
Basic to observe, the Continuous Observation of preventing seabed base and buoy system presents the complete procedure that anoxic occurs, develops and withered away, and makes me
To the understanding of anoxic more comprehensively and deeply.Bottom Dissolved Oxygen in Water is consumed and cannot effectively mended by organic matter decomposition
Fill is that the observation such as mechanism, big face station, preventing seabed base and buoy that anoxic occurs is dissolved oxygen is comprehensive in various environmental factors in water body
Instant concentration under cooperation, it is the interaction result of organic matter decomposition oxygen consumption and vertical mixing oxygenating, if can obtain
Oxygen consumption and oxygenating process and speed under the conditions of varying environment, that can is grasped and the degree of prediction water hypoxia and change, this
The MATTER IN CHANGJIANG ESTUARY ecosystem is studied significant.Basic weak, the starting evening that China is studied due to ocean observation technology,
Observation for seabed water body organic matter oxygen process is also only limitted to laboratory simulation culture experiment, lacks and carries out in-situ batch culture reality
The platform tested.Although simulated experiment is kept and live identical environment, including temperature, salinity and light as far as possible, essence is intended to
The natural environment at scene is really reappeared, is nearly impossible, so the field observation technical research in situ of oxygen process seems urgent
Cut and significant.
The content of the invention
The technical problem to be solved in the present invention is：Overcome the deficiencies in the prior art, there is provided one kind can be placed directly in seabed,
The water body oxygen process Culture in situ system and its application method used under benthon nature living environment.
To achieve the above object, present invention employs following technical scheme：
The present invention provides a kind of Culture in situ system, including mechanical structure framework and automatically controlled processing module, the machinery knot
Structure framework includes culture vessel device, sample liquid harvester and lowering or hoisting gear, and the automatically controlled processing module includes power module, place
Device module, direct current generator circuit module and stepper motor circuit module are managed, the sample liquid harvester includes injection device, sampling
Device, console, measurement sensor, control side plate, stepper motor, transmission belt, rolling rod, motion bar, locator and flexible bullet
Spring II.
Wherein：The injection device is the organic matter solution for injecting the default density into container；The culture is held
Device device includes being used for the container for gathering sample liquid, and the lowering or hoisting gear is arranged on console, and the container is fixedly mounted on control
Platform lower surface processed, the lowering or hoisting gear can drive container to move up and down；The automatically controlled processing module is fixedly installed on liter
On falling unit, stepper motor circuit module is connected with stepper motor；Direct current generator circuit module and measurement sensor and processor
Module is connected, and processor module, direct current generator circuit module are connected with power module respectively；The measurement sensor is fixedly mounted
In console, the sampling apparatus is connected with container, and sampling apparatus is used to carry out sampling processing to the sample liquid gathered in container.
Rolling rod is connected by transmission belt and stepper motor, and the rolling rod is provided with runner, the top of motion bar
It is connected with locator, the bottom of motion bar can offset with runner, and the one side of the locator from control side plate extends through it
Another side, runner can be rotated with rolling rod, and motion bar is connected with control side plate so that control side plate can
Support motion bar prizes, and prizing locator locator by motion bar can be along on the direction of control side plate
Carry out telescopic moving；The sampling apparatus is connected with the sampling body of rod by sampling cavity and formed, and sampling cavity is by conduit with holding
Device is connected；The sampling cavity is fixed on control side plate, and sampling body of rod top can be carried out along wall in sampling cavity body
Slide up and down, reagent is pushed away so as to realize the pull sample liquid of sampling apparatus or squeeze；The sampling body of rod bottom and adjustable spring II
Connection, the bottom of adjustable spring II are fixed on control side plate, and telescopic direction and the sampling body of rod of adjustable spring II move up and down
Direction is identical；The notch that can be used in the buckle sampling body of rod is offered on one end of the locator；When the sampling body of rod is buckled in
In notch on locator, now adjustable spring II is in extended state, drives rolling rod to rotate by starting stepper motor, together
When drive the runner on rolling rod to be rotated, the runner in rotation process can stir the motion bar to be offseted with the runner, living
Lever prizes locator and enables locator to carry out telescopic moving along on the direction of control side plate, so that adopting
The sample body of rod is separated from the notch of locator, is moved downward in the stretching action down-sampling body of rod of adjustable spring II, realization is adopted
Sample cavity pull sample liquid out of container.
Preferably：The sample liquid harvester also includes stirring motor, and the culture vessel device also includes stirring
Impeller is mixed, direct current generator circuit module is connected with stirring motor, and direct current generator circuit module is connected with power module respectively；It is described
Stirring motor is fixedly mounted on console, and agitator arm is arranged in container, and agitator arm is electrically connected with stirring motor, is stirred
Motor is mixed to be used to control agitator arm to be stirred motion to the sample liquid gathered in container.
Preferably：The culture vessel device also includes the base that matches with container, be provided with base with
The screwed hole that the external screw thread II that connecting rod bottom is provided with matches, the first evenly distributed bottom of several spacing is additionally provided with base
Block hole, these first base through holes can effectively reduce seawater resistance during whole device or system decentralization seawater experiment
Power acts on, and greatly improves lowering velocity.
Preferably：The console includes control bottom plate, ring washer and controlroof, and control bottom plate passes through ring
Shape packing ring is fixedly connected with controlroof；The ring washer be arranged at control bottom plate and controlroof between mainly play it is close
Seal connection effect；The top and bottom of the container are uncovered structure, and the upper end of container is fixedly mounted on control bottom plate, held
The lower end of device can extend through the second base through hole set in base.
Preferably：Stirring motor extends through the first through hole set in controlroof and extends downwardly and be arranged on
Agitator arm drive connection in container, is provided with first annular groove, first annular in the first through hole upper end of controlroof
The ring washer for being used for being tightly connected effect is equipped with groove so that stirring motor is sealedly connected on control by ring washer
On top plate.
Preferably：The measurement sensor extends through the second through hole set in controlroof and extended downwardly into
In container, it is connected and sealed between measurement sensor and controlroof by fastening clamp ring and gasket ring block, fastening clamp ring is set
In gasket ring block upper end, if arterial highway elastic washer I arranged in parallel is equipped with the gasket ring through-hole wall of gasket ring block, and in gasket ring
Elastic washer II is respectively equipped with the upper and lower surface of block, axle center and the gasket ring through hole in gasket ring block of these elastic washers II
Axle center be located on same axial line；The equal semicircular in shape in the cross section of elastic washer I and elastic washer II or half elliptic.
Wherein：Elastic washer II is mainly to realize the connection sealing effectiveness between controlroof, and elastic washer I is mainly
Realize the connection sealing effectiveness between measurement sensor.
Preferably：The lowering or hoisting gear includes support, connecting rod, sleeve pipe I, sleeve pipe II and adjustable spring III, described
Support is fixedly mounted on the control bottom plate of console；Upper collar and lower collar are provided with the support, in upper collar and lower top
Through hole and lower through-hole are respectively equipped with circle, the upper collar is connected by sleeve pipe II with lower collar, and upper through hole and sleeve pipe II
It is interconnected between the through hole of sleeve pipe II and lower through-hole that are set between the through hole of sleeve pipe II inside set and in sleeve pipe II；The connecting rod
The through hole of sleeve pipe I, upper through hole, the through hole of sleeve pipe II, lower through-hole and the adjustable spring by being set in sleeve pipe I are sequentially passed through from top to bottom
III, external screw thread I, external screw thread II are respectively equipped with the top of connecting rod and bottom, wherein：The external screw thread II of connecting rod bottom can be the bottom of with
Seat is threadedly coupled simultaneously in the base, and the top of adjustable spring III offsets with control bottom plate, the bottom of adjustable spring III and base
Offset；The nut I to match is provided with the external screw thread I on connecting rod top.
Specifically, can be by being rotated down twisting nut I, in spiral shell in lowering or hoisting gear is moved down into operating process
After the top of mother I and sleeve pipe I offsets, as nut I continues rotation twisting so that connecting rod stretches out upwards, simultaneous retractable spring III
Compression gradually shortens after being squeezed, i.e., now lowering or hoisting gear moves gradually downward, while lowering or hoisting gear drives container to move down
And extend downwardly through by second base through hole on base.
Preferably：Through hole II is provided with control bottom plate, and connecting rod extends through the through hole II, the lower top on support
Circle offsets with control bottom plate；It ensure that and remain steady status between lowering or hoisting gear and control bottom plate so that connecting rod bottom exists
After being connected with whorl of base, by the way that lowering or hoisting gear is moved down while drives container to move down so that the lower end of container is passed through
The second base through hole set in base is crossed in break-through, so that container gathers and forms the sample liquid of stable state.
Preferably：Adjustable spring I is arranged with locator, one end and the locator A ends phase of the adjustable spring I
Support, the notch that the other end of adjustable spring I is provided with locator B ends offsets；In adjustable spring I carries out telescopic process, make
Obtain and mutually draw close or be located remotely from each other between locator A ends and locator B ends；The support member that motion bar is provided with control side plate
Be flexibly connected, the support member be realize motion bar prize during support zone, when the runner on rolling rod and motion bar one
When end offsets, the motion bar other end is connected with locator, and now the thief rod body of sampling apparatus can stably be buckled in positioning
In the notch at device B ends；The runner on rolling rod is driven to be rotated starting stepper motor so that motion bar one end, which is separated from, to be turned
Wheel, the motion bar other end prizes locator immediately, under the effect of adjustable spring I so that stretched toward locator A ends at locator B ends
Draw close, so that the sampling body of rod of sampling apparatus departs from the notch at locator B ends, the sampling body of rod of sampling apparatus is in flexible bullet
Spring II moves down under acting on, and realizes that sampling cavity extracts enough sample liquids.
The present invention also provides a kind of application method of Culture in situ system, specifically includes following operating procedure：
First passing through lowering or hoisting gear drives container to move down so that container extends through the second base through hole of base, connects
And transfer to whole Culture in situ system in predetermined maritime area, after base reaches predetermined maritime area bottom surface stabilization, start measurement and pass
Sensor and stirring motor, stirring motor drives the sample liquid being arranged in container, while passes through sample in measurement sensor detection container
The parameter of liquid；
Then starting stepper motor drives rolling rod to rotate, while drives the runner on rolling rod to be rotated, and rotates
Runner in journey stirs the motion bar to be offseted with the runner, and motion bar prizes locator and causes locator along perpendicular to control side
Telescopic moving is carried out on the direction of plate, so that the sampling body of rod is separated from the notch of locator, in the stretching of adjustable spring II
The effect down-sampling body of rod is moved downward, and realizes pull sample liquid in the sampling calm device of cavity.
Preferably：The measurement sensor is to use RBR-XR420 multi-parameter water quality instrument, is configured with temperature, salt
5 degree, depth, dissolved oxygen and turbidity parameters.
Preferably：The sampling apparatus includes several syringes, wherein：Sample the injection that cavity is syringe
Cavity, the sampling body of rod are the push handle of syringe.
Compared with prior art, the beneficial effects of the invention are as follows：
For the present invention due to using above-mentioned technical scheme, Culture in situ system is one applied to seabed water body oxygen process
Cover automatic observation and culture device, integral electronic control module, motor, container, seawater measurement sensor and other machinery knots
Structure module is integrated, and drops to seabed by self gravitation, container forms confined space with bottom sediment interface, positioned at container
Interior measurement sensor continuously measures dissolved oxygen and ambient parameter data, the continuous measurement simultaneously of the measurement sensor outside container
Outside body of water dissolved oxygen and ambient parameter data；Sample liquid harvester is injected by above-mentioned mechanical structure device into container to be preset
The organic matter solution (can also select to be not injected into any solution, simple to cultivate the oxygen process of water body in itself) of concentration value, interval
Sample liquid collector extracts the sample liquid of fixed volume out of container after set time, to last recovery post analysis nutritive salt and organic
The parameters such as carbon.Culture in situ experiment can from a few houres to several days, and Dissolved Oxygen in Water can all have one inside and outside culture vessel
Time-varying process, with reference to other ambient parameter datas so as to understanding the oxygen process of water body under site environment.
Brief description of the drawings
Fig. 1 is a kind of overall structure diagram of Culture in situ system in the present invention.
Fig. 2 is Fig. 1 configuration schematic diagram.
Fig. 3 is the configuration schematic diagram of lowering or hoisting gear in the present invention.
Fig. 4 is that one of one side mounting structure schematic diagram of side is controlled in the present invention.
Fig. 5 is two of the one side mounting structure schematic diagram of control side in the present invention.
Fig. 6 is the another side mounting structure schematic diagram that side is controlled in the present invention.
Fig. 7 is the fit structure schematic diagram between the sampling body of rod and notch in invention.
Fig. 8 is the configuration schematic diagram of console in the present invention.
Fig. 9 is the fit structure schematic diagram between measurement sensor and console in the present invention.
Figure 10 is the structural representation of gasket ring block in the present invention.
Reference：
Automatically controlled processing module 1, lowering or hoisting gear 2, support 201, upper collar 201A, lower collar 201B, connecting rod 202, external screw thread I
202A, the 202B of external screw thread II, sleeve pipe I 203, sleeve pipe II 204, adjustable spring III 205, nut 206A, nut 206B；
Sampling module 3 is injected, side plate 301 is controlled, stepper motor 302, transmission belt 303, rolling rod 304, runner 305, lives
Lever 306, locator 307, notch 3071, adjustable spring I 308, support member 309, cavity 310 is sampled, sample the body of rod 311, stretch
Contracting spring II 312, conduit 313；
Control bottom plate 401, through hole I 401A, annular groove 401B, the 401C of through hole II, stirring motor 402, measurement sensor
403, ring washer 404, controlroof 405, first annular groove 406, first through hole 407, the second through hole 408, fasten clamp ring
409, gasket ring block 410, the 410B of elastic washer I, the 410A of elastic washer II, gasket ring through hole 410C；
Agitator arm 501, container 502, base 503, first base through hole 504, second base through hole 505.
Embodiment
The embodiment of the present invention is made a detailed explanation below in conjunction with the accompanying drawings.
Embodiment：
As shown in Fig. 1~10, the present invention provides a kind of specific embodiment of Culture in situ system and its application method, such as schemes
Shown in 1~2, the Culture in situ system in the present invention includes mechanical structure framework and automatically controlled processing module 1, the mechanical structure frame
Frame includes culture vessel device, sample liquid harvester and lowering or hoisting gear, and the automatically controlled processing module 1 includes power module, processing
Device module, direct current generator circuit module and stepper motor circuit module, the sample liquid harvester include injection device, sampling cartridge
Put, console, measurement sensor 403, control side plate 301, stepper motor 302, transmission belt 303, rolling rod 304, motion bar
306th, locator 307 and adjustable spring II 312.
Wherein：The injection device is the organic matter solution for injecting the default density into container；Specifically, this hair
Bright middle injection device is using on control side plate 301 and for injecting the default density organic matter solution into container
Syringe.
The culture vessel device includes being used for the container 502 for gathering sample liquid, and the lowering or hoisting gear is arranged on console,
The container 502 is fixedly mounted on console lower surface, and the lowering or hoisting gear can drive container 502 to move up and down；Institute
State automatically controlled processing module 1 to be fixedly installed on lowering or hoisting gear 2, stepper motor circuit module is connected with stepper motor 302；Direct current
Login module and measurement sensor 403 are connected with processor module, processor module, direct current generator circuit module respectively with electricity
Source module connects；The measurement sensor 403 is fixedly mounted on console, and the sampling apparatus is connected with container 502, sampling
Device is used to carry out sampling processing to the sample liquid of collection in container 502；The sampling processing is mainly by the sample liquid in container 502
It is drawn into sampling apparatus, enough sample liquids is left in order to which the needs of follow-up work carry out backup.
As shown in fig. 6, the rolling rod 304 is connected by transmission belt 303 and stepper motor 301, the rolling rod
304 are provided with runner 305, and the top of motion bar 306 is connected with locator 307, and the bottom of motion bar 306 can be with runner 305
Offset, the one side of the locator 307 from control side plate 301 extends through its another side, and runner 305 can be with rolling rod
304 are rotated, and motion bar 306 is connected with control side plate 301 so that control side plate 301 can support motion bar 306
Prize, prizing the locator 307 of locator 307 by motion bar 306 can be along on the direction of control side plate 301
Carry out telescopic moving.The sampling apparatus is made up of sampling cavity 310 and the sampling connection of the body of rod 311, and sampling cavity 310 passes through
Conduit 313 is connected with container 502；The sampling cavity 310 is fixed on control side plate 301, and the sampling body of rod 311 top can
Slided up and down along the sampling internal face of cavity 310, reagent is pushed away so as to realize the pull sample liquid of sampling apparatus or squeeze；It is described
The sampling bottom of the body of rod 311 is connected with adjustable spring II 312, and the bottom of adjustable spring II 312 is fixed on control side plate 301, is stretched
To move up and down direction identical with the sampling body of rod 311 for the telescopic direction of contracting spring II 312；Opened up on one end of the locator 307
There is the notch 3071 that can be used in the buckle sampling body of rod 311；The notch 3071 being buckled in when the sampling body of rod 311 on locator 307
Interior, now adjustable spring II 312 is in extended state, drives rolling rod 304 to rotate by starting stepper motor 302, while band
Runner 305 on dynamic rolling rod 304 is rotated, and the runner 305 in rotation process can stir the work to be offseted with the runner 305
Lever 306, motion bar 306 prize locator 307 and enable locator 307 along on the direction of control side plate 301
Telescopic moving is carried out, so that the sampling body of rod 311 is separated from the notch 3071 of locator 307, in the drawing of adjustable spring II 312
Stretch the effect down-sampling body of rod 311 to be moved downward, realize the sampling pull sample liquid out of container 502 of cavity 310.
Specifically：Sampling apparatus is mainly made up of side by side several syringes in embodiments of the present invention, wherein：Injection
Device is connected with push handle by injection cavity and formed, and the injection cavity is fixed on control side plate 301, and push handle top can be along
Wall is slided up and down in injecting cavity body, and reagent is pushed away so as to realize the pull sample liquid of sampling apparatus or squeeze；The push handle bottom
End is connected with adjustable spring II 312, and the bottom of adjustable spring II 312 is fixed on control side plate 301, adjustable spring II 312
It is identical that telescopic direction with push handle moves up and down direction.Offered on one end of the locator 307 and can be used in buckle push handle
Notch 3071；When in the notch 3071 that push handle is buckled on locator 307, now adjustable spring II 312 is in extended state, lead to
Crossing startup stepper motor 302 drives rolling rod 304 to rotate, while drives the runner 305 on rolling rod 304 to be rotated, and rotates
During runner 305 can stir the motion bar 306 to be offseted with the runner 305, motion bar 306 prizes locator 307 and caused
Locator 307 can carry out telescopic moving along on the direction of control side plate 301, so that push handle is separated from positioning
The notch 3071 of device 307, push handle is moved downward under the stretching action of adjustable spring II 312, realizes that injection cavity is calm
Pull sample liquid realizes sampling in device 502.
The sample liquid harvester also includes stirring motor 402, and the culture vessel device also includes agitator arm 501,
Direct current generator circuit module is connected with stirring motor 402, and direct current generator circuit module is connected with power module respectively；The stirring
Motor 402 is fixedly mounted on console, and agitator arm 501 is arranged in container 502, agitator arm 501 and stirring motor 402
Electrical connection, stirring motor 402 are used to control agitator arm 501 to be stirred motion to the sample liquid gathered in container 502.
As shown in figure 1, the culture vessel device also includes the base 503 to match with container 502, on base 503
The screwed hole to match provided with the 202B of external screw thread II being provided with the bottom of connecting rod 202, is additionally provided with several spacing on base 503
Evenly distributed first base through hole 504, these first base through holes 504 are in whole device or system decentralization seawater experiment process
In can effectively reduce seawater drag effect, greatly improve lowering velocity.
As illustrated in figures 1 and 8, the console includes control bottom plate 401, ring washer 404 and controlroof 405, control
Bottom plate 401 processed is fixedly connected by ring washer 404 with controlroof 405；The ring washer 404 is arranged at control bottom plate
Mainly sealed connection effect is played between 401 and controlroof 405；The top and bottom of the container 502 are open knot
Structure, the upper end of container 502 are fixedly mounted on control bottom plate 401, and the lower end of container 502 can extend through to be set in base 503
Second base through hole 505.
Stirring motor 402 extends through the first through hole 407 set in controlroof 405 and extends downwardly and be arranged on container
Agitator arm 501 in 502 is connected, and first annular groove 406 is provided with the upper end of first through hole 407 of controlroof 405,
The ring washer for being used for being tightly connected effect is equipped with first annular groove 406 so that stirring motor 402 passes through air ring
Circle is sealedly connected on controlroof 405.
As shown in Fig. 9~10, the measurement sensor 403 extends through the second through hole 408 set in controlroof 405 simultaneously
Extend downwardly into container 502, by fastening clamp ring 409 and gasket ring block 410 between measurement sensor 403 and controlroof 405
It is connected and sealed, fastening clamp ring 409 is arranged on the upper end of gasket ring block 410, the ring on the gasket ring through hole 410C inwalls of gasket ring block 410
If provided with the arterial highway 410B of elastic washer I arranged in parallel, and elasticity is respectively equipped with the upper and lower surface of gasket ring block 410
The 410A of packing ring II, these elastic washers II 410A axle center and the axle center of gasket ring through hole 410C in gasket ring block 410 are located at same axle
On heart line；The 410B of the elastic washer I and 410A of the elastic washer II equal semicircular in shape in cross section or half elliptic.
Wherein：The 410A of elastic washer II is mainly to realize the connection sealing effectiveness between controlroof 405, elastic washer
I 410B is mainly to realize the connection sealing effectiveness between measurement sensor 403.
As shown in figure 3, the lowering or hoisting gear 2 includes support 201, connecting rod 202, sleeve pipe I 203, sleeve pipe II 204 and flexible bullet
Spring III 205, the support 201 are fixedly mounted on the control bottom plate 401 of console；Upper collar is provided with the support 201
201A and lower collar 201B, through hole and lower through-hole, the upper collar are respectively equipped with upper collar 201A and lower collar 201B
201A is connected by sleeve pipe II 204 with lower collar 201B, and between the through hole of sleeve pipe II set in upper through hole and sleeve pipe II 204, with
And it is interconnected between the through hole of sleeve pipe II and lower through-hole set in sleeve pipe II 204；The connecting rod 202 sequentially passes through logical from top to bottom
The through hole of sleeve pipe I set, upper through hole, the through hole of sleeve pipe II, lower through-hole and adjustable spring III 205 are crossed in sleeve pipe I 203, in connecting rod 202
Top and bottom are respectively equipped with the 202A of external screw thread I, the 202B of external screw thread II, wherein：The 202B of external screw thread II of the bottom of connecting rod 202 can
It is threadedly coupled with base 503 and in base 503, the 206B of nut II of matching is provided with the 202B of external screw thread II；It is flexible
Bottom plate 401 offsets on the top of spring III 205, the bottom of adjustable spring III 205 and base 503 offset with control；On the top of connecting rod 202
The 202A of external screw thread I is provided with the 206A of nut I to match.
Specifically, in lowering or hoisting gear 2 is moved down into operating process, the 206A of nut I can be twisted by being rotated down,
After being offseted on the 206A of nut I and sleeve pipe I 203 top, as the 206A of nut I continues rotation twisting so that connecting rod 202 is stretched upwards
Go out, simultaneous retractable spring III 205 compresses after being squeezed gradually to shorten, i.e., now lowering or hoisting gear 2 moves gradually downward, lifts simultaneously
Device 2 drives container 502 to move down and extend downwardly through by second base through hole 505 on base 503.
As shown in figure 8, being provided with the 401C of through hole II on control bottom plate 401, connecting rod 202 extends through the through hole II 401C,
Lower collar 201B on support 201 offsets with control bottom plate 401；It ensure that and protected all the time between lowering or hoisting gear 2 and control bottom plate 401
Hold steady status so that the bottom of connecting rod 202 with base 503 after being threadedly coupled, by the way that lowering or hoisting gear 2 is moved down while band
Visibly moved device 502 moves down so that and the lower end of container 502 extends through the second base through hole 505 set in base 503, so as to
So that container 502 gathers and forms the sample liquid of stable state.
As also shown in e.g. figs. 4-7, adjustable spring I 308 is arranged with locator 307, one end of the adjustable spring I 308 is with determining
Position device 307A ends offset, and the notch 3071 that the other end of adjustable spring I 308 is provided with locator 307B ends offsets；Flexible
Spring I 308 is carried out in telescopic process so that is mutually drawn close between locator 307A ends and locator 307B ends or mutually remote
From；The support member 309 that motion bar 306 is provided with control side plate 301 is flexibly connected, and the support member 309 is to realize motion bar 306
Support zone during prizing, when the runner 305 on rolling rod 304 offsets with the one end of motion bar 306, motion bar 306 is another
One end is connected with locator 307, and now the sampling body of rod 311 on sampling apparatus can stably be buckled in locator 307B ends
In notch 3071；The runner 305 on rolling rod 304 is driven to be rotated starting stepper motor 302 so that motion bar 306 1
End is separated from runner 305, and the other end of motion bar 306 prizes locator 307 immediately, under the effect of adjustable spring I 308 so that fixed
Position device 307B ends are drawn close toward locator 307A ends are flexible, so that the sampling body of rod 311 of sampling apparatus departs from locator 307B
The notch 3071 at end, the sampling body of rod 311 of sampling apparatus move down under the effect of adjustable spring II 312, realize sampling cavity
310 extract enough sample liquids.
The embodiment of the present invention also provides a kind of application method of Culture in situ system, specifically includes following operating procedure：
First passing through lowering or hoisting gear 2 drives container 502 to move down so that container 502 extends through the second bottom of base 503
Block hole 505, then whole Culture in situ system is transferred in predetermined maritime area, it is steady to treat that base 503 reaches predetermined maritime area bottom surface
After fixed, start measurement sensor 403 and stirring motor 402, stirring motor 402 drives the sample liquid being arranged in container 502, simultaneously
Pass through the parameter of sample liquid in the detection container 502 of measurement sensor 403；
Then starting stepper motor 302 drives rolling rod 304 to rotate, while drives the runner 305 on rolling rod 304 to carry out
Rotate, the runner 305 in rotation process stirs the motion bar 306 to be offseted with the runner 305, and motion bar 306 prizes locator 307
So that locator 307 carries out telescopic moving along on the direction of control side plate 301, so that the sampling body of rod 311 is de-
The notch 3071 of locator 307 is left, is moved downward in the stretching action down-sampling body of rod 311 of adjustable spring II 312, it is real
Now sample the pull sample liquid out of container 502 of cavity 310.
Wherein：The measurement sensor 403 is to use RBR-XR420 multi-parameter water quality instrument, is configured with temperature, salinity, depth
5 degree, dissolved oxygen and turbidity parameters.
The general principle and principal character and advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the simply explanation described in above-described embodiment and specification is originally
The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (10)
1. a kind of Culture in situ system, it is characterised in that including mechanical structure framework and automatically controlled processing module (1), the machinery
Structural framing includes culture vessel device, sample liquid harvester and lowering or hoisting gear, and the automatically controlled processing module (1) includes power supply mould
Block, processor module, direct current generator circuit module and stepper motor circuit module, the sample liquid harvester include sampling cartridge
Put, console, measurement sensor (403), control side plate (301), stepper motor (302), transmission belt (303), rolling rod
(304), motion bar (306), locator (307) and adjustable spring II (312), the culture vessel device include being used to gather sample
The container (502) of liquid, the lowering or hoisting gear are arranged on console, and the container (502) is fixedly mounted on console lower surface,
The lowering or hoisting gear can drive container (502) to move up and down；The automatically controlled processing module (1) is fixedly installed on lifting dress
Put, stepper motor circuit module is connected with stepper motor (302)；Direct current generator circuit module and measurement sensor (403) with
Processor module is connected, and processor module, direct current generator circuit module are connected with power module respectively；The measurement sensor
(403) it is fixedly mounted on console, the sampling apparatus is connected with container (502), and sampling apparatus is used for container (502)
The sample liquid of interior collection carries out sampling processing；
Rolling rod (304) is connected by transmission belt (303) and stepper motor (302), and the rolling rod (304), which is provided with, to be turned
Take turns (305), the top of motion bar (306) is connected with locator (307), and the bottom of motion bar (306) can be with runner (305) phase
Support, the locator (307) extends through its another side from the one side of control side plate (301), and runner (305) can be with rolling
Lever (304) is rotated, and motion bar (306) is connected with control side plate (301) so that control side plate (301) can prop up
Support motion bar (306) prizes, and prizing locator (307) locator (307) by motion bar (306) can be along vertical
In control side plate (301) direction on carry out telescopic moving；
The sampling apparatus is made up of sampling cavity (310) and the sampling body of rod (311) connection, and sampling cavity (310) is by leading
Pipe (313) is connected with container (502)；The sampling cavity (310) is fixed on control side plate (301), samples the body of rod (311)
Top can be slided up and down along sampling cavity (310) internal face, so as to realize the pull sample liquid of sampling apparatus or squeeze
Push away reagent；The sampling body of rod (311) bottom is connected with adjustable spring II (312), and the bottom of adjustable spring II (312) is fixed on
Control on side plate (301), to move up and down direction identical with sampling the body of rod (311) for the telescopic direction of adjustable spring II (312)；It is described
The notch (3071) that can be used in the buckle sampling body of rod (311) is offered on one end of locator (307)；When the sampling body of rod
(311) it is buckled in the notch (3071) on locator (307) Nei, now adjustable spring II (312) is in extended state, by opening
Dynamic stepper motor (302) drives rolling rod (304) to rotate, while drives the runner (305) on rolling rod (304) to be rotated,
Runner (305) in rotation process can stir the motion bar (306) to be offseted with the runner (305), and motion bar (306) prizes fixed
Position device (307) causes locator (307) to carry out telescopic moving along on the direction of control side plate (301), so as to
So that the sampling body of rod (311) is separated from the notch (3071) of locator (307), under the stretching action of adjustable spring II (312)
The sampling body of rod (311) is moved downward, and realizes sampling cavity (310) from the interior pull sample liquid of container (502)；
The sample liquid harvester also includes stirring motor (402), and direct current generator circuit module is connected with stirring motor (402),
Stirring motor (402) is used to be stirred motion to the sample liquid gathered in container (502).
2. according to a kind of Culture in situ system described in claim 1, it is characterised in that the culture vessel device also includes
The base (503) to match with container (502), the external screw thread II being provided with connecting rod (202) bottom is provided with base (503)
The screwed hole that (202B) matches, the evenly distributed first base through hole of several spacing is additionally provided with base (503)
(504)。
3. according to a kind of Culture in situ system described in claim 2, it is characterised in that the culture vessel device also includes
Agitator arm (501), the stirring motor (402) are fixedly mounted on console, and agitator arm (501) is arranged on container
(502) in, agitator arm (501) is electrically connected with stirring motor (402), and stirring motor (402) is used to control agitator arm
(501) motion is stirred to the sample liquid gathered in container (502).
4. according to a kind of Culture in situ system described in claim 2, it is characterised in that the console includes control bottom plate
(401), ring washer (404) and controlroof (405), control bottom plate (401) pass through ring washer (404) and controlroof
(405) it is fixedly connected；The top and bottom of the container (502) are uncovered structure, and the upper end of container (502) is fixedly mounted on
Control on bottom plate (401), the lower end of container (502) can extend through the second base through hole (505) set in base (503).
5. according to a kind of Culture in situ system described in claim 4, it is characterised in that stirring motor (402) extends through
The interior first through hole (407) set of controlroof (405) simultaneously extends downwardly and is arranged on the agitator arm (501) in container (502)
Drive connection, is provided with first annular groove (406), in first annular groove in first through hole (407) upper end of controlroof (405)
Ring washer is equipped with ditch (406) so that stirring motor (402) is sealedly connected on controlroof (405) by ring washer
On.
6. according to a kind of Culture in situ system described in claim 4, it is characterised in that the measurement sensor (403) is passed through
Break-through is crossed in controlroof (405) the second through hole (408) set and extended downwardly into container (502), measurement sensor (403)
It is connected and sealed between controlroof (405) by fastening clamp ring (409) and gasket ring block (410), fastening clamp ring (409) is set
Put in gasket ring block (410) upper end, it is arranged in parallel if being equipped with arterial highway on inwall in the gasket ring through hole (410C) of gasket ring block (410)
Elastic washer I (410B), and elastic washer II (410A) is respectively equipped with the upper and lower surface of gasket ring block (410), this
A little axle center of elastic washer II (410A) are located on same axial line with the axle center of the interior gasket ring through hole (410C) of gasket ring block (410)；
The equal semicircular in shape in cross section or half elliptic of elastic washer I (410B) and elastic washer II (410A).
7. according to a kind of Culture in situ system described in claim 1, it is characterised in that the lowering or hoisting gear (2) includes branch
Frame (201), connecting rod (202), sleeve pipe I (203), sleeve pipe II (204) and adjustable spring III (205), the fixed peace of the support (201)
On console；Upper collar (201A) and lower collar (201B) are provided with the support (201), upper collar (201A) and under
Through hole and lower through-hole are respectively equipped with collar (201B), the upper collar (201A) passes through sleeve pipe II (204) and lower collar
(201B) is connected, and the set set between the through hole of sleeve pipe II set in upper through hole and sleeve pipe II (204) and in sleeve pipe II (204)
It is interconnected between the through hole of pipe II and lower through-hole；
The connecting rod (202) sequentially passes through the through hole of sleeve pipe I, upper through hole, the sleeve pipe II by being set in sleeve pipe I (203) from top to bottom
Through hole, lower through-hole and adjustable spring III (205), external screw thread I (202A), outer is respectively equipped with the top of connecting rod (202) and bottom
Screw thread II (202B), wherein：The external screw thread II (202B) of connecting rod (202) bottom can be threadedly coupled and set with base (503)
In the base (503), and adjustable spring III (205) top offsets with control bottom plate (401), adjustable spring III (205) bottom with
Base (503) offsets；The nut I (206A) to match is provided with the external screw thread I (202A) on connecting rod (202) top.
8. according to a kind of Culture in situ system described in claim 7, it is characterised in that be provided with through hole II on console
(401C), connecting rod (202) extend through the through hole II (401C), and the lower collar (201B) on support (201) offsets with console；
So that connecting rod (202) bottom is with base (503) after being threadedly coupled, by being moved down lowering or hoisting gear while driving container
(502) moving down so that the lower end of container (502) extends through the second base through hole (505) set in base (503), from
And container (502) is gathered and forms the sample liquid of stable state.
9. according to a kind of Culture in situ system described in claim 8, it is characterised in that be arranged with locator (307)
Adjustable spring I (308), one end and locator (307) A ends of the adjustable spring I (308) offset, adjustable spring I (308) it is another
The notch (3071) that one end is provided with locator (307) B ends offsets；In adjustable spring I (308) carries out telescopic process, make
Obtain and mutually draw close or be located remotely from each other between locator (307) A ends and locator (307) B ends；Motion bar (306) and control side
The support member (309) that plate (301) is provided with is flexibly connected, during the support member (309) realizes that motion bar (306) prizes
Support zone, when the runner (305) on rolling rod (304) offsets with motion bar (306) one end, motion bar (306) other end
It is connected with locator (307), now the sampling body of rod (311) of sampling apparatus can stably be buckled in locator (307) B ends
In notch (3071)；Starting stepper motor (302) drives the runner (305) on rolling rod (304) to be rotated so that motion bar
(306) one end is separated from runner (305), and motion bar (306) other end prizes locator (307) immediately, in adjustable spring I
(308) under acting on so that locator (307) B ends are drawn close toward locator (307) A ends are flexible, so that the sampling of sampling apparatus
The body of rod (311) departs from the notch (3071) at locator (307) B ends, and the sampling body of rod (311) of sampling apparatus is in adjustable spring II
(312) moved down under acting on, realize that sampling cavity (310) extracts enough sample liquids.
10. the application method based on a kind of Culture in situ system described in claim 9, it is characterised in that specifically include as follows
Operating procedure：
First passing through lowering or hoisting gear drives container (502) to move down so that container (502) extends through the second of base (503)
Base plate through holes (505), then whole Culture in situ system is transferred in predetermined maritime area, treat that base (503) reaches predetermined maritime area
After bottom surface is stable, start measurement sensor (403) and stirring motor (402), stirring motor (402) drives and is arranged on container
(502) sample liquid in, while pass through the parameter of measurement sensor (403) detection container (502) interior sample liquid；
Then starting stepper motor (302) drives rolling rod (304) to rotate, while drives the runner (305) on rolling rod (304)
Rotated, the runner (305) in rotation process stirs the motion bar (306) to be offseted with the runner (305), motion bar (306)
Prize locator (307) so that locator (307) along perpendicular to control side plate (301) direction on carry out telescopic moving, from
And make it that the sampling body of rod (311) is separated from the notch (3071) of locator (307), in the stretching action of adjustable spring II (312)
The down-sampling body of rod (311) is moved downward, and realizes sampling cavity (310) from the interior pull sample liquid of container (502).
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| CN201610335478.9A CN106018720B (en) | 2016-05-19 | 2016-05-19 | Culture in situ system and its application method |
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|---|---|---|---|
| CN201610335478.9A CN106018720B (en) | 2016-05-19 | 2016-05-19 | Culture in situ system and its application method |
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| CN112130212A (en) * | 2020-08-21 | 2020-12-25 | 中国石油天然气集团有限公司 | Bearing body structure of marine electromagnetic node acquisition station |
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