CN104498323A - Deep sea in-situ microbial cultivation apparatus - Google Patents

Deep sea in-situ microbial cultivation apparatus Download PDF

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Publication number
CN104498323A
CN104498323A CN201410660062.5A CN201410660062A CN104498323A CN 104498323 A CN104498323 A CN 104498323A CN 201410660062 A CN201410660062 A CN 201410660062A CN 104498323 A CN104498323 A CN 104498323A
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Prior art keywords
culture tank
gland
fixed
sliding sleeve
baffle ring
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CN201410660062.5A
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CN104498323B (en
Inventor
周东辉
邵宗泽
董纯明
刘军
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Hangzhou Dianzi University
Third Institute of Oceanography SOA
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Hangzhou Dianzi University
Third Institute of Oceanography SOA
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Manufacture And Refinement Of Metals (AREA)

Abstract

The invention discloses a deep sea in-situ microbial cultivation apparatus. The deep sea specific microbe enrichment research equipment is insufficient. According to the invention, a sliding sleeve is fixed on bottom of a mounting rack, outer wall of a hanging pipe enables clearance fit with an upper shield ring and a lower shield ring; top of a guiding pipe is fixed with the lower shield ring, the bottom is connected to the mounting rack through a tension spring; the guiding pipe is connected to a sliding sleeve in a sliding mode; several isolation nets are arranged in a culture pot with equal distance, a threaded rod passes through all centers of the isolation nets; the nuts are arranged at upper side and lower side of the isolation net, the nut and the threaded rod enable threaded connection for fixing the isolation net; several culture pots arranged in the mounting rack, the culture pot is fixed with the sliding sleeve; a culture pot gland is arranged on top of the culture pot; each culture pot gland is fixed with one end of a gland lever; central section of each gland lever is hinged to a lever connecting block of the corresponding culture pot, and the other end stretches into a gap of the upper shield ring and a lower shield ring. The deep sea in-situ microbial cultivation apparatus can be placed under deep sea environment for long term, and is used for enrichment culture and recovery of microbe.

Description

A kind of deep sea in-situ microbial cultivation device
Technical field
The invention belongs to Deep-Sea Microorganisms technical field, be specifically related to a kind of deep sea in-situ microbial cultivation device.
Background technology
In recent years, in the world to international waters genetic resources most attention, ocean genetic resources has great potential using value.Because advanced country in the world is engaged in the history that abyssopelagic organism the Study on Resources has had many decades, establish zonal observation grid at deep-sea, develop a series of ecotope, the long-established stations such as physical chemistry of the earth, long-term observation and sampling can be carried out to deep-sea floor Biological resources and environment, not only take advantage on equipment technology, have also obtained scientific discovery new in a large number and abyssopelagic organism environmental resources data and sample, substantially complete and be engaged in the necessary primitive accumulation of deep-sea extreme environment scientific research, for its exploitation to the deep-sea extreme environment ecosystem and resource thereof are laid a good foundation.
Deep-Sea Microorganisms resource not only relies on deep sea in-situ sampling and obtains, and also needs to utilize deep sea in-situ environment to carry out field planting for goal in research and cultivates enrichment and sampling.In pelagic deposit substance environment, need to adopt settling Culture in situ system, carry out the Culture in situ of microorganism in deep-sea floor Sediment environment.Being cultivated by example enrichment and obtain original position microbiological specimens, is the deep-sea processes of further microorganisms participation and the powerful of acquisition genetic resources, an importance of relevant contexts change and Protective strategy in Ye Shi international mining area, seabed application.Existing deep-sea specified microorganisms enrichment research equipment is not enough, and therefore, the deep sea in-situ microbial cultivation device developing a kind of enrichment culture and recovery microorganism becomes exigence.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, a kind of deep sea in-situ microbial cultivation device is provided, can place for a long time under deep-marine-environment, for enrichment culture and the recovery of microorganism.
The present invention includes erecting frame, sliding sleeve, sliding catheter, culture tank, culture tank gland and gland lever.Described sliding sleeve is fixed on bottom erecting frame, and middle part symmetry offers two chutes, and bottom outer wall is welded with the polylith culture tank web plate be uniformly distributed along the circumference.
Described sliding catheter comprises swing pipe, upper baffle ring, lower baffle ring and guide pipe; The bottom outer wall of described swing pipe from top to bottom successively with upper baffle ring and lower baffle ring running fit, upper baffle ring and lower baffle ring spacing are arranged; Top and the lower baffle ring of described guide pipe are fixed, and bottom is nested with and is connected with one end of extension spring in sliding sleeve; The other end of extension spring is connected with erecting frame; The zygomorphy of guide pipe is fixed with two pieces of slide blocks, and the chute that two pieces of slide blocks are corresponding with sliding sleeve is respectively connected by sliding pair.
The top exterior walls of described culture tank is welded with lever contiguous block, and bottom outer wall is welded with sliding sleeve web plate, and inside is equidistantly provided with multiple separation nets, and threaded rod is through the center of all separation nets; The upper and lower both sides of described separation net are equipped with nut, and nut is threaded with threaded rod and is fixed by separation net; The top of culture tank is provided with culture tank gland.Multiple culture tank is arranged in erecting frame, and the quantity of culture tank is equal with the quantity of culture tank web plate; The sliding sleeve web plate of each culture tank and one piece of culture tank web plate of sliding sleeve are fixed; One end of each culture tank gland and a gland lever is fixed; The middle part of every root gland lever is hinged with the lever contiguous block of corresponding culture tank, and the other end stretches in the gap between the upper baffle ring of sliding catheter and lower baffle ring.
Described sliding catheter also comprises pull bar and suspension ring; Described pull bar entirety is in inverted U-shaped, and two-arm is fixed on the top exterior walls of swing pipe; Described suspension ring latch closure is on pull bar.
Wear ring is provided with between described culture tank and culture tank gland.
The material of all parts is seawater corrosion resistance material, as titanium alloy or tetrafluoroethylene.
Beneficial effect of the present invention:
1, the present invention breaches the key core technology that Deep-Sea Microorganisms biological sample collection, in-situ observation and field planting are cultivated, can land in seabed for a long time, original position microorganism enrichment culture is carried out in deep sea surface layer deposit sediment, and realize pollution-free sample collecting, water body environment parameter of the nearly end can be recorded, for the specific function microorganism obtained in settling provides seabed experiment porch simultaneously.
2, the present invention is made by the titanium material of seawater corrosion resistance and polytetrafluoroethylmaterial material, can be placed on seabed for a long time, pollution-free to microorganism enrichment.
3, the present invention by latent device as ROV or manned submersible deep ocean work reclaim.
4, the present invention will become one of China's important deep sea equipment in deep-sea activety fault and protection, improves the acquisition capability of China's biomass based production, for the strategic resource deposit increasing country provides technical support.
Accompanying drawing explanation
Fig. 1 is one-piece construction stereographic map of the present invention;
Fig. 2 is local structure stereographic map of the present invention;
Fig. 3 is one-piece construction sectional view of the present invention;
Fig. 4 is the structure sectional view of sliding catheter in the present invention;
Fig. 5 is the sectional arrangement drawing with cultivation each part tank connected in the present invention.
Embodiment
Below in conjunction with drawings and Examples, the invention will be further described.
As illustrated in fig. 1 and 2, a kind of deep sea in-situ microbial cultivation device, comprises erecting frame 1, sliding sleeve 2, sliding catheter 3, culture tank 4, culture tank gland 5 and gland lever 6.Sliding sleeve 2 is fixed on bottom erecting frame 1, and middle part symmetry offers two chute 2-1, and bottom outer wall is welded with the eight pieces of culture tank web plate 2-2 be uniformly distributed along the circumference.
As shown in Fig. 2,3 and 4, sliding catheter 3 comprises pull bar 3-1, swing pipe 3-2, suspension ring 3-3, upper baffle ring 3-4, lower baffle ring 3-5 and guide pipe 3-6; Pull bar 3-1 entirety is in inverted U-shaped, and two-arm is fixed on the top exterior walls of swing pipe 3-2, and suspension ring 3-3 latch closure is on pull bar 3-1; The bottom outer wall of swing pipe 3-2 from top to bottom successively with upper baffle ring 3-4 and lower baffle ring 3-5 running fit, upper baffle ring 3-4 and lower baffle ring 3-5 spacing are arranged; Top and the lower baffle ring 3-5 of guide pipe 3-6 fix, and bottom is nested with and is connected with one end of extension spring 7 in sliding sleeve 2; The other end of extension spring 7 is connected with erecting frame 1; The zygomorphy of guide pipe 3-6 is fixed with two pieces of slide block 3-7, and the chute 2-1 that two pieces of slide block 3-7 are corresponding with sliding sleeve is respectively connected by sliding pair.
As shown in Fig. 2,3 and 5, the top exterior walls of culture tank 4 is welded with lever contiguous block 4-1, and bottom outer wall is welded with sliding sleeve web plate 4-2, and inside is equidistantly provided with three separation net 4-3, and threaded rod 4-4 is through the center of three separation net 4-3; The upper and lower both sides of separation net 4-3 are equipped with nut, and nut is threaded with threaded rod 4-4 and is fixed by separation net 4-3; The top of culture tank 4 is provided with culture tank gland 5, is provided with wear ring 4-5 between culture tank 4 and culture tank gland 5.Eight culture tank 4 are arranged in erecting frame 1, and the sliding sleeve web plate 4-2 of each culture tank and one piece of culture tank web plate 2-2 of sliding sleeve is bolted; One end of each culture tank gland 5 and a gland lever 6 is fixed; The middle part of every root gland lever 6 is hinged with the lever contiguous block 4-1 of corresponding culture tank, and the other end stretches in the gap between the upper baffle ring 3-4 of sliding catheter and lower baffle ring 3-5.
The material of all parts is seawater corrosion resistance material, as titanium alloy or tetrafluoroethylene.
The principle of work of this deep sea in-situ microbial cultivation device:
The layering of multiple-microorganism solid medium can be placed on the separation net 4-3 in culture tank 4, block can not deviate from from culture tank 4 by separation net, thus orienting enriching microorganism.By suspension ring 3-3 sling this culture apparatus time, pull bar 3-1 drives swing pipe 3-2 to move upward, erecting frame 1 and culture tank 4 are due to action of gravity sinking, lower baffle ring 3-5 lifts one end of gland lever 6, the other end of gland lever 6 drives culture tank gland 5 to be pressed on the tank mouth of culture tank 4, realizes Jar opening sealer; Now, guide pipe 3-6 stretching extension spring 7; After unclamping suspension ring 3-3, pull bar 3-1 declines because deadweight and extension spring 7 act on, and drives one end of gland lever 6 to decline, and the other end of gland lever 6 drives culture tank gland 5 to open, and seawater can be made to enter in tank.
This deep sea in-situ microbial cultivation device is made by the titanium material of seawater corrosion resistance and polytetrafluoroethylmaterial material, can be placed on seabed for a long time, pollution-free to microorganism enrichment; By latent device as ROV or manned submersible deep ocean work reclaim.

Claims (4)

1. a deep sea in-situ microbial cultivation device, comprise erecting frame, sliding sleeve, sliding catheter, culture tank, culture tank gland and gland lever, it is characterized in that: described sliding sleeve is fixed on bottom erecting frame, middle part symmetry offers two chutes, and bottom outer wall is welded with the polylith culture tank web plate be uniformly distributed along the circumference;
Described sliding catheter comprises swing pipe, upper baffle ring, lower baffle ring and guide pipe; The bottom outer wall of described swing pipe from top to bottom successively with upper baffle ring and lower baffle ring running fit, upper baffle ring and lower baffle ring spacing are arranged; Top and the lower baffle ring of described guide pipe are fixed, and bottom is nested with and is connected with one end of extension spring in sliding sleeve; The other end of extension spring is connected with erecting frame; The zygomorphy of guide pipe is fixed with two pieces of slide blocks, and the chute that two pieces of slide blocks are corresponding with sliding sleeve is respectively connected by sliding pair;
The top exterior walls of described culture tank is welded with lever contiguous block, and bottom outer wall is welded with sliding sleeve web plate, and inside is equidistantly provided with multiple separation nets, and threaded rod is through the center of all separation nets; The upper and lower both sides of described separation net are equipped with nut, and nut is threaded with threaded rod and is fixed by separation net; The top of culture tank is provided with culture tank gland; Multiple culture tank is arranged in erecting frame, and the quantity of culture tank is equal with the quantity of culture tank web plate; The sliding sleeve web plate of each culture tank and one piece of culture tank web plate of sliding sleeve are fixed; One end of each culture tank gland and a gland lever is fixed; The middle part of every root gland lever is hinged with the lever contiguous block of corresponding culture tank, and the other end stretches in the gap between the upper baffle ring of sliding catheter and lower baffle ring.
2. a kind of deep sea in-situ microbial cultivation device according to claim 1, is characterized in that: described sliding catheter also comprises pull bar and suspension ring; Described pull bar entirety is in inverted U-shaped, and two-arm is fixed on the top exterior walls of swing pipe; Described suspension ring latch closure is on pull bar.
3. a kind of deep sea in-situ microbial cultivation device according to claim 1, is characterized in that: be provided with wear ring between described culture tank and culture tank gland.
4. a kind of deep sea in-situ microbial cultivation device according to claim 1,2 or 3, is characterized in that: the material of all parts is seawater corrosion resistance material, as titanium alloy or tetrafluoroethylene.
CN201410660062.5A 2014-11-18 2014-11-18 A kind of deep sea in-situ microbial cultivation device Active CN104498323B (en)

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104789449A (en) * 2015-04-27 2015-07-22 国家海洋局第一海洋研究所 In-situ culturing device for deep-sea microbes
CN105236559A (en) * 2015-11-25 2016-01-13 绍兴文理学院 Device for purifying eutrophic water body through microbial compound bacteria
CN105400688A (en) * 2015-11-16 2016-03-16 尚超 Marine microorganism in-situ enrichment device
CN105758687A (en) * 2016-04-13 2016-07-13 杭州电子科技大学 Deep-sea sediment in-situ cultivation and hermetic sampling mechanism
CN105802833A (en) * 2016-04-13 2016-07-27 杭州电子科技大学 Deep sea in-situ suspension culture tank
CN105861277A (en) * 2016-04-21 2016-08-17 同济大学 Underwater injection-type sediment in-situ culture device
CN105865834A (en) * 2016-04-13 2016-08-17 杭州电子科技大学 Controllable sealed deep sea sediment colonized culture sampling mechanism
CN110055156A (en) * 2018-06-07 2019-07-26 南方科技大学 Deep-Sea Microorganisms water body in-situ culture apparatus
CN110157607A (en) * 2019-06-03 2019-08-23 中国农业科学院农田灌溉研究所 Soil microbial community characteristic reaction device and measurement device
CN110317723A (en) * 2019-08-05 2019-10-11 中国地质大学（北京） It can self-sealing Deep-Sea Microorganisms original position field planting enrichment culture tank
CN112616757A (en) * 2020-12-25 2021-04-09 中国科学院海洋研究所 ROV-based deep sea in-situ large-scale organism culture device and use method thereof
CN112791645A (en) * 2021-01-26 2021-05-14 驻马店职业技术学院 Fluid-driven control valve

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CN201449345U (en) * 2009-07-03 2010-05-05 河海大学 Multi-directional water sample collection device
CN103011040A (en) * 2012-12-21 2013-04-03 重庆大学 Device for automatically opening cap of vacuum blood collection container
CN103484360A (en) * 2013-09-09 2014-01-01 中国船舶重工集团公司第七一〇研究所 Circulation type culture cabin for deep-sea microorganisms
CN103674675A (en) * 2013-07-26 2014-03-26 山东省科学院海洋仪器仪表研究所 Enrichment device and method for organic pollutants in seawater

Patent Citations (5)

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Publication number Priority date Publication date Assignee Title
CN1752732A (en) * 2004-09-22 2006-03-29 中国科学院沈阳自动化研究所 Air-water mixture reaches plankton sampling thief under water
CN201449345U (en) * 2009-07-03 2010-05-05 河海大学 Multi-directional water sample collection device
CN103011040A (en) * 2012-12-21 2013-04-03 重庆大学 Device for automatically opening cap of vacuum blood collection container
CN103674675A (en) * 2013-07-26 2014-03-26 山东省科学院海洋仪器仪表研究所 Enrichment device and method for organic pollutants in seawater
CN103484360A (en) * 2013-09-09 2014-01-01 中国船舶重工集团公司第七一〇研究所 Circulation type culture cabin for deep-sea microorganisms

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104789449A (en) * 2015-04-27 2015-07-22 国家海洋局第一海洋研究所 In-situ culturing device for deep-sea microbes
CN105400688A (en) * 2015-11-16 2016-03-16 尚超 Marine microorganism in-situ enrichment device
CN105236559A (en) * 2015-11-25 2016-01-13 绍兴文理学院 Device for purifying eutrophic water body through microbial compound bacteria
CN105758687B (en) * 2016-04-13 2018-09-07 杭州电子科技大学 A kind of cultivation of halmeic deposit original position and sealing sampling mechanism
CN105802833A (en) * 2016-04-13 2016-07-27 杭州电子科技大学 Deep sea in-situ suspension culture tank
CN105865834A (en) * 2016-04-13 2016-08-17 杭州电子科技大学 Controllable sealed deep sea sediment colonized culture sampling mechanism
CN105758687A (en) * 2016-04-13 2016-07-13 杭州电子科技大学 Deep-sea sediment in-situ cultivation and hermetic sampling mechanism
CN105861277A (en) * 2016-04-21 2016-08-17 同济大学 Underwater injection-type sediment in-situ culture device
CN105861277B (en) * 2016-04-21 2017-12-15 同济大学 Underwater injecting type deposit Culture in situ device
CN110055156A (en) * 2018-06-07 2019-07-26 南方科技大学 Deep-Sea Microorganisms water body in-situ culture apparatus
CN110157607A (en) * 2019-06-03 2019-08-23 中国农业科学院农田灌溉研究所 Soil microbial community characteristic reaction device and measurement device
CN110317723A (en) * 2019-08-05 2019-10-11 中国地质大学（北京） It can self-sealing Deep-Sea Microorganisms original position field planting enrichment culture tank
CN110317723B (en) * 2019-08-05 2023-12-19 中国地质大学（北京） Deep sea microorganism in-situ field planting enrichment culture tank capable of being automatically sealed
CN112616757A (en) * 2020-12-25 2021-04-09 中国科学院海洋研究所 ROV-based deep sea in-situ large-scale organism culture device and use method thereof
CN112791645A (en) * 2021-01-26 2021-05-14 驻马店职业技术学院 Fluid-driven control valve
CN112791645B (en) * 2021-01-26 2022-05-13 驻马店职业技术学院 Fluid-driven control valve

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