CN213549124U - Long time sequence deep sea in-situ large-scale organism stress culture device - Google Patents
Long time sequence deep sea in-situ large-scale organism stress culture device Download PDFInfo
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- CN213549124U CN213549124U CN202022351802.0U CN202022351802U CN213549124U CN 213549124 U CN213549124 U CN 213549124U CN 202022351802 U CN202022351802 U CN 202022351802U CN 213549124 U CN213549124 U CN 213549124U
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Abstract
The utility model relates to a long-time-sequence deep-sea in-situ large-scale organism stress culture device, a power supply control barrel, a circulating pump, a peristaltic pump, a stress culture barrel body and a sample bag are respectively placed on a placing plate, a fixing hoop is arranged on the stress culture barrel body, one side of an upper cover is connected with the stress culture barrel body in a relatively rotatable way, the other side is provided with an upper cover switch pressing block, a switch lower end pressing block is arranged on the fixing hoop, and the upper cover switch pressing block is connected with the switch lower end pressing block in a clamping way when the upper cover is closed with the stress culture barrel body; the sample bag is communicated with the inlet of the peristaltic pump through a sampling pipe, and the outlet of the peristaltic pump is communicated with the interior of the stress culture barrel body through an injection pipe; one end of the circulating pump is communicated with the interior of the stress culture barrel body through a circulating water inlet pipe, and the other end of the circulating pump is connected with a circulating water obtaining pipe for replacing external seawater; two ends of the tension spring are respectively connected with the upper cover and the placing plate. The utility model discloses it is little influenced by the sample depth, and the work is nimble stable to can acquire, cultivate, coerce, fixed sample effectively for a long time.
Description
Technical Field
The utility model belongs to the research field of deep sea normal position macrobiotic stress experiments, in particular to a long-time sequence deep sea normal position macrobiotic stress culture device.
Background
The deep sea cold spring energy ecosystem has unique chemical environment and ocean dynamic characteristics, is highly similar to the environment in the early earth life forming period, breeds a unique ecosystem and life process, and is an important target area for revealing the origin, evolution and extreme environment adaptation mechanism of life. Under the continuous support of deep sea detection platforms of various units in China, the characteristics, the biological composition and the potential functions of a deep sea cold spring energy ecological system are preliminarily recognized; however, due to the lack of a reliable large-scale organism in-situ experimental platform and the difficulty in realizing the continuous dynamic observation and mechanism analysis of the long-term deep sea key life process, the method becomes a bottleneck problem which is concerned by research teams at home and abroad and restricts the deep sea research and development for a long time.
The in-situ experiment in the large-scale organism stress culture aspect is further deeply and systematically researched by combining the existing research experience at home and abroad and utilizing the precise positioning capability of an ROV (remote operated vehicle), so that the problem to be solved urgently is solved.
SUMMERY OF THE UTILITY MODEL
To the technical bottleneck problem that present deep sea life science research meets, the utility model aims to provide a culture apparatus is coerced to long-time series deep sea normal position macrobiosis.
The purpose of the utility model is realized through the following technical scheme:
the utility model discloses a power control bucket, circulating pump, peristaltic pump and deep sea macrobiosis culture apparatus, wherein deep sea macrobiosis culture apparatus includes upper cover, fixed hoop, place set, coerces and cultivates staving, extension spring and sample bag, power control bucket, circulating pump, peristaltic pump, coerces and cultivates staving and sample bag and places respectively on placing the set, install the fixed hoop on the coerces and cultivates the staving, one side of upper cover and coerces and cultivates the staving and be connected with relatively rotating, the opposite side is equipped with upper cover switch briquetting, install switch lower extreme briquetting on the fixed hoop, upper cover switch briquetting and switch lower extreme briquetting joint when upper cover and coerces and cultivates the staving are closed; the sample bag is communicated with an inlet of a peristaltic pump through a sampling pipe, and an outlet of the peristaltic pump is communicated with the interior of the stress culture barrel body through an injection pipe; one end of the circulating pump is communicated with the interior of the stress culture barrel body through a circulating water inlet pipe, and the other end of the circulating pump is connected with a circulating water obtaining pipe for replacing external seawater; an underwater control unit and a power supply unit are arranged in the power supply control barrel, the power supply unit is connected with the underwater control unit and supplies power to the underwater control unit, a control barrel control terminal is arranged on the power supply control barrel and is connected with the underwater control unit, and the peristaltic pump and the circulating pump are respectively connected with the control barrel control terminal through a control barrel control cable; two ends of the tension spring are respectively connected with the upper cover and the placing plate.
Wherein: the upper cover switch pressing block is provided with a pressing block upper end tooth, the switch lower end pressing block is divided into a switch lower end pressing block A and a switch lower end pressing block B, the switch lower end pressing block A is fixedly connected to the fixing hoop, the switch lower end pressing block B is rotatably connected with the switch lower end pressing block A through a rotating shaft, a torsion spring is sleeved on the rotating shaft, and two pressing edges of the torsion spring are respectively abutted to the switch lower end pressing block A and the switch lower end pressing block B; and the upper end teeth of the pressing block on the upper cover switch pressing block are meshed with the lower end teeth of the switch on the switch lower end pressing block B when the upper cover and the stress culture barrel body are closed.
One side of the upper cover is connected with the coercion culture barrel body through a damping hinge, one end of the damping hinge is fixedly connected to the upper cover, and the other end of the damping hinge is fixedly connected to the coercion culture barrel body.
And a connecting line between the center of the projection of one end of the damping hinge on the upper cover and the center of the projection of the upper cover switch pressing block on the upper cover passes through the circle center of the upper cover.
One end of the damping hinge is connected with a hinge extension plate, an upper end fixing ring is arranged on the lower surface of the hinge extension plate, a lower end fixing ring is arranged on the placing disc, and two ends of the tension spring are respectively hooked on the upper end fixing ring and the lower end fixing ring.
The upper cover is provided with a handle A, a one-way valve and a stop ball valve respectively.
The fixed hoop is divided into an upper end fixed hoop and a lower end fixed hoop, the switch lower end pressing block is installed on the upper end fixed hoop, and the handle B which is obliquely arranged and is convenient for the ROV manipulator to grab is installed on the lower end fixed hoop.
A sealing gasket is arranged on the inner side of the upper cover, and the outer edge of the sealing gasket is smaller than that of the upper cover; when the upper cover is closed and compressed, the sealing gasket is in sealing and abutting connection with the stress culture barrel body.
The peristaltic pump is arranged on the placing disc through a peristaltic pump fixing frame, and the circulating pump is fixed on the placing disc through a circulating pump mounting frame; the control barrel control terminal on the power supply control barrel is divided into a control barrel control terminal A and a control barrel control terminal B, the peristaltic pump is connected with the control barrel control terminal A through a control barrel control cable A, and the underwater control unit controls the peristaltic pump to pump the base liquid in the sample bag into the stress culture barrel body through the sampling tube and the injection tube; the circulating pump is connected with a control barrel control terminal B through a control barrel control cable B, the underwater control unit controls the circulating pump, and external seawater acquired through the circulating water acquisition pipe is pumped into the stress culture barrel body through the circulating water inlet pipe.
The utility model discloses an advantage does with positive effect:
the utility model discloses integrated acquisition, long time sequence cultivation, long time sequence stress, fixed deep sea macrobiosis normal position experimental apparatus satisfies the symbiosis of macrobiosis in the deep sea energy ecosystem, long time sequence stress and than surveys the experiment demand, knows the material energy acquisition and the transmission mode of deep sea symbiosis system through isotope adding cultivation; the specific long time sequence stress experiment is carried out in situ, the stress response mechanism is analyzed, the stress culture device is slightly influenced by the sampling depth, the corrosion resistance is high, the work is flexible and stable, and the samples can be quickly, effectively and quickly obtained, cultured in a long time sequence, stressed and fixed.
Drawings
FIG. 1 is a schematic view of the working principle of the present invention;
FIG. 2 is a schematic view of the overall structure of the present invention;
FIG. 3 is a schematic perspective view of the stress cultivation barrel body of FIG. 2 and components mounted thereon;
fig. 4 is a structural bottom view of the upper cover of the present invention;
FIG. 5 is a schematic structural view of the torsion spring of the present invention;
FIG. 6 is a schematic view of the internal structure of the peristaltic pump of the present invention;
wherein: 1 is a handle A, 2 is a one-way valve, 3 is a stop ball valve, 4 is a damping hinge, 5 is an upper end fixing ring, 6 is a hinge extension plate, 7 is an upper end fixing hoop, 8 is a lower end fixing hoop, 9 is a handle B, 10 is a placing disc, 11 is a tension spring, 12 is a peristaltic pump fixing rack, 13 is a lower end fixing ring, 14 is a peristaltic pump, 15 is an injection tube, 16 is an injection port, 17 is a sampling tube, 18 is a sample bag, 19 is an upper cover, 20 is an upper cover port of the one-way valve, 21 is an upper cover port of the stop ball valve, 22 is an inner edge of a sealing gasket, 23 is a sealing gasket, 24 is an outer edge of the sealing gasket, 25 is an upper cover opening and closing pressing block, 26 is a pressing block upper end tooth, 27 is a switch lower end tooth, 28 is a rotating shaft hole, 29 is a switch lower end pressing block A, 30 is a torsion spring pressing edge A, 31 is torsion spring pressing edge B, 32 is a switch lower end pressing block B, 33, 36 is the power control bucket, 37 is control bucket control terminal A, 38 is control bucket control terminal B, 39 is control bucket control cable A, 40 is control bucket control cable B, 41 is the circulating pump, 42 is the circulating pump mounting bracket, 43 is the circulation mouth of a river, 44 is the circulating water inlet tube, 45 is the circulating water and obtains the pipe, 46 is the peristaltic pump clamp plate, 47 is the peristaltic pump water pipe, 48 is the peristaltic pump gyro wheel, 49 is the peristaltic pump head, 50 is the peristaltic pump bolt, 51 is the peristaltic pump spring.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings.
As shown in fig. 1-6, the utility model discloses a power control bucket 36, circulating pump 41, peristaltic pump 14 and deep sea macrobiosis culture apparatus, wherein deep sea macrobiosis culture apparatus includes upper cover 19, the fixed hoop, place the dish 10, coerce culture staving 35, extension spring 11 and sample bag 18, power control bucket 36, circulating pump 41, peristaltic pump 14, coerce culture staving 35 and sample bag 18 are placed respectively on placing the dish 10, install the fixed hoop on the coerce culture staving 35, one side of upper cover 19 and coerce culture staving 35 are connected with relatively rotating, the opposite side is equipped with upper cover switch briquetting 25, install switch lower extreme briquetting on the fixed hoop, upper cover switch briquetting 25 with switch lower extreme briquetting joint when upper cover 19 and coerce culture staving 35 are closed; an outlet at the lower end of the peristaltic pump 14 is connected with one end of an injection pipe 15, the other end of the injection pipe 15 is connected with an injection port 16, the injection port 16 is in threaded connection with the middle lower part of the stress culture barrel body 35, an inlet at the upper end of the peristaltic pump 14 is connected with one end of a sampling pipe 17, the other end of the sampling pipe 17 is connected with a sample bag 18, and the sample bag 18 is fixed on the placing disc 10; one end of the circulating pump 41 is communicated with the interior of the stress cultivation barrel body 35 through a circulating water inlet pipe 44 and a circulating water port 43, and the other end is connected with a circulating water obtaining pipe 45 for replacing external seawater; an underwater control unit and a power supply unit are arranged in the power supply control barrel 36, the power supply unit is connected with the underwater control unit and supplies power to the underwater control unit, a control barrel control terminal is arranged on the power supply control barrel 36 and is connected with the underwater control unit, and the peristaltic pump 14 and the circulating pump 41 are respectively connected with the control barrel control terminal through a control barrel control cable; two ends of the tension spring 11 are respectively connected with the upper cover 19 and the placing disc 10.
The power control bucket 36 of this embodiment uses the bolt fastening on rotary disk 10, and the top surface of power control bucket 36 is equipped with control bucket control terminal A37 and control bucket control terminal B38 respectively, and the control unit (the utility model discloses a control unit is prior art under water) and the power supply unit concentrate and place in power control bucket 36. The underwater control unit is used for power supply control, circulating pump time sequence control, peristaltic pump time sequence control and detection control, the power supply control is used for starting and stopping the power supply and protecting current, the time sequence control is used for finishing actions of the circulating pump 41 and the peristaltic pump 14 according to a set time sequence, and the detection control is used for finishing current and voltage detection of the power supply, the circulating pump 41 and the peristaltic pump 14. The peristaltic pump 14 of the embodiment is mounted on the placing tray 10 through a peristaltic pump fixing frame 12 by using bolts, and the circulating pump 41 is fixed on the placing tray 10 through a circulating pump mounting frame 42 by using bolts; the control barrel control terminal on the power control barrel 36 is divided into a control barrel control terminal A37 and a control barrel control terminal B38, the peristaltic pump 14 is connected with the control barrel control terminal A37 through a control barrel control cable A39, the underwater control unit controls the peristaltic pump 14 to pump the bottom liquid in the sample bag 18 into the stress culture barrel body 35 through the sampling pipe 17 and the injection pipe 15, the rotating speed and the time of the peristaltic pump 14 are controllable, and therefore the injection quantity value is controllable; circulating pump 41 links to each other with control barrel control terminal B38 through control barrel control cable B40, by underwater control unit control circulating pump 41, will acquire external sea water through circulating water acquisition pipe 45 and pump into coercion cultivation staving 35 in through circulating water inlet tube 44, the ROV can carry out the hand-held type operation and accomplish the sea water exchange in specific area, and circulating pump 41's rotational speed and time are controllable, so it is controllable to change the water quantity value.
The upper cover 19 of this embodiment is circular, and one side of upper cover 19 is passed through damping hinge 4 and is cultivateed the staving 35 with coercing and link to each other, and this damping hinge 4's one end welds on upper cover 19, and the other end utilizes the bolt rigid coupling to cultivate on staving 35 with coercing. Hinge extension plate 6 is welded to the one end of damping hinge 4, and the lower surface of this hinge extension plate 6 is equipped with the solid fixed ring of upper end 5, is equipped with the solid fixed ring of lower extreme 13 on placing the dish 10, and the both ends of extension spring 11 are respectively hooked on solid fixed ring of upper end 5 and the solid fixed ring of lower extreme 13.
The fixed hoop of this embodiment divide into upper end fixed hoop 7 and lower extreme fixed hoop 8, welds upper end fixed hoop 7 on the duress cultivation staving 35 of 6 tail end lower parts of hinge extension plate, welds handle B9 on the fixed hoop 8 of lower extreme, and this handle B9 slope sets up, and the ROV manipulator of being convenient for snatchs. The injection tube 15 and the sampling tube 17 of this embodiment are both manufactured by saint-gobain, france, the stress cultivation barrel 35 is made of non-metal material (such as teflon), and the sample bag 18 is made of soft material (such as plastic).
The upper cover switch pressing block 25 of the embodiment is provided with a pressing block upper end tooth 26. The switch lower end pressing block of the embodiment is divided into a switch lower end pressing block A29 and a switch lower end pressing block B32, the switch lower end pressing block A29 is welded on the upper end fixing hoop 7, the switch lower end pressing block B32 and the switch lower end pressing block A29 fix a rotating shaft 33 by utilizing a rotating shaft hole 28, the switch lower end pressing block B32 is rotatably connected with the switch lower end pressing block A29 through the rotating shaft 33, a torsion spring 34 is sleeved on the rotating shaft 33, two pressing edges (namely a torsion spring pressing edge A30 and a torsion spring pressing edge B31) of the torsion spring 34 are respectively abutted against the switch lower end pressing block A29 and the switch lower end pressing block B32, the torsion spring 34 keeps tension, and the switch lower end pressing block A29 and the switch; the switch lower end pressing block B32 is provided with a switch lower end tooth 27, and the pressing block upper end tooth 26 on the upper cover switch pressing block 25 is meshed with the switch lower end tooth 27 on the switch lower end pressing block B32 when the upper cover 19 is closed with the stress cultivation barrel body 35. The connecting line between the center of the projection of one end of the damping hinge 4 on the upper cover 19 and the center of the projection of the upper cover switch pressing block 25 on the upper cover 19 passes through the center of the upper cover 19, so that the pressing block upper end teeth 26 on the upper end switch pressing block 25 are meshed with the switch lower end teeth 27 on the switch lower end pressing block B32 conveniently in the rotating process of the damping hinge 4.
The inner side of the upper cover 19 of the embodiment is provided with a sealing gasket 23, and the outer edge 24 of the sealing gasket 23 is smaller than the outer edge of the upper cover 19; when the upper cover 19 is closed and pressed, the sealing gasket 23 is contacted with the stress cultivation barrel body 35, and the sealing function is completed. The upper cover 19 is respectively provided with a check valve upper cover port 20 and a stop ball valve upper cover port 21 which are respectively connected with the check valve 2 and the stop ball valve 3 by screw threads, so that the liquid in the forced culture barrel body 35 can be discharged through the check valve 2, and oxygen can be supplied into the rib forced culture barrel body 35 through the stop ball valve 3. The check valve 2 and the stop ball valve 3 of the embodiment are made of metal materials (such as Ti alloy), so that the pollution of samples is avoided. The middle position of the upper cover 1 is welded with a handle A1, which is convenient for the ROV manipulator to grab.
In the peristaltic pump 14 of this embodiment, a plurality of peristaltic pump rollers 48 are uniformly arranged on a motor-driven roller along the circumferential direction, an arc-shaped peristaltic pump pressing plate 46 is installed above the roller, a peristaltic pump water pipe 47 passes through between the peristaltic pump rollers 48 and the peristaltic pump pressing plate 46, and two ends of the peristaltic pump water pipe 47 are respectively communicated with the sampling pipe 17 and the injection pipe 15. A peristaltic pump head 49 above the peristaltic pump pressure plate 46 is mounted on the pump housing through a peristaltic pump bolt 50, can move up and down, and is always abutted against the peristaltic pump pressure plate 46 under the action of a peristaltic pump spring 51. The process of pumping the base solution in the sample bag 18 into the stress cultivation barrel 35 by the peristaltic pump 14 is prior art and will not be described in detail herein.
The utility model discloses a use method does:
firstly, integrally disassembling and cleaning a shore-based end; decomposing and disassembling the stress culture device integrally, and then cleaning;
step two, assembling after cleaning, wherein in an initial state, the upper cover 19 is in an open state, and the sample bag 18 is in a base solution loading state;
thirdly, an ROV is used for carrying the stress culture device to reach a fixed point position for operation, an ROV manipulator is used for grabbing large deep sea organisms and placing the large deep sea organisms into the stress culture barrel body 35, an ROV manipulator is used for grabbing a handle A1 and further closing the upper cover 19, the upper end teeth 26 of the pressing block on the upper cover switch pressing block 25 and the lower end teeth 27 of the switch on the switch lower end pressing block B32 are meshed together, and when the upper cover 19 is closed, the upper cover 19 and the stress culture barrel body 35 are sealed and closed;
after a set period of time, the underwater control unit in the power control barrel 36 controls the circulating pump 41 to work, external seawater is obtained through the circulating water obtaining pipe 45 and then enters the stress cultivation barrel body 35 through the circulating water inlet pipe 44 and the circulating water port 43, liquid in the stress cultivation barrel body 35 can be discharged through the one-way valve 2, the rotating speed and the time of the circulating pump 41 are controllable, and therefore the water changing quantity value is controllable; the water body in the stress culture barrel body 35 and the external environment of the deep sea are subjected to circulating exchange, and the exchange time is related to the volume of the stress culture barrel body 35 and the flow rate of the circulating pump 41;
step five, the underwater control unit in the power supply control barrel 36 controls the peristaltic pump 14 to rotate, the peristaltic pump 14 pumps the base solution in the sample bag 18 into the stress culture barrel body 35 through the sampling pipe 17, the peristaltic pump water pipe 47, the injection pipe 15 and the injection port 16, the liquid in the stress culture barrel body 35 can be discharged through the one-way valve 2, the rotating speed and the time of the peristaltic pump 14 are controllable, sequential reciprocating circulation can be carried out, the injection is stopped after the design quantification, and the stress culture of large organisms is carried out;
and step six, repeating the step four and the step five according to the time setting. Until the experimental requirements are met;
and seventhly, opening the upper cover 19 by using an ROV manipulator, grabbing a handle B9 by using the ROV manipulator, pouring the sample in the stress culture barrel body 35, pouring the sample into the in-situ fixing device for sample fixing, and then recovering the sample carried by the ROV.
The utility model provides an in situ fixing device can adopt the "macrobiosis extrusion formula in situ fixing device such as deep sea mussel based on ROV" that the grant bulletin date is 2020 year 7 month 17 days, grant bulletin number is CN 211042749U.
The utility model discloses to the technological bottleneck of present deep sea life science research, break through the accurate base liquid of normal position long term sequence and annotate and get the technique, break through the normal position closed system under quick uncap, the normal position is fixed key technology such as under water, finally accomplish long term sequence normal position experiment, solve the not enough and time efficiency of china's macrobiosis normal position experiment, be difficult to the problem that macrobiosis normal position coerces cultivation, fixed transfer. The utility model discloses an coerce culture apparatus combines the ROV operation under water, steady operation, use the ROV to conveniently carry, do not receive the restriction of ROV activity duration simultaneously, and applicable in the 6000m degree of depth, the complicated submarine environment under temperature and the ocean current environment, the integration acquires, long time series cultivates, the function that long time series was coerced, final cooperation fixing device, satisfy the intergrowth of macrobiosis among the deep-sea energy ecosystem, long time series flank compels and than survey the experiment demand, and coerce culture apparatus and receive the sample depth to influence for a short time, corrosion resisting property is strong, the flexible operation is stable, and can be quick, effectively, acquire, long time series is cultivateed, long time series is coerced, fixed sample.
Claims (9)
1. The utility model provides a long-time sequence deep sea normal position macrobiosis coerces culture apparatus which characterized in that: comprises a power supply control barrel (36), a circulating pump (41), a peristaltic pump (14) and a deep-sea large-scale organism culture device, wherein the deep-sea large-scale organism culture device comprises an upper cover (19), a fixed hoop, a placing disc (10), a stress culture barrel body (35), a tension spring (11) and a sample bag (18), the power supply control barrel (36), the circulating pump (41), the peristaltic pump (14), the stress culture barrel body (35) and the sample bag (18) are respectively arranged on the placing tray (10), the stress cultivation barrel body (35) is provided with a fixed hoop, one side of the upper cover (19) is connected with the stress cultivation barrel body (35) in a relatively rotatable way, the other side is provided with an upper cover switch pressing block (25), the fixed hoop is provided with a switch lower end pressing block, and the upper cover switch pressing block (25) is clamped with the switch lower end pressing block when the upper cover (19) and the stress culture barrel body (35) are closed; the sample bag (18) is communicated with an inlet of a peristaltic pump (14) through a sampling pipe (17), and an outlet of the peristaltic pump (14) is communicated with the interior of the stress culture barrel body (35) through an injection pipe (15); one end of the circulating pump (41) is communicated with the interior of the stress cultivation barrel body (35) through a circulating water inlet pipe (44), and the other end of the circulating pump is connected with a circulating water obtaining pipe (45) for replacing external seawater; an underwater control unit and a power supply unit are arranged in the power supply control barrel (36), the power supply unit is connected with the underwater control unit and supplies power to the underwater control unit, a control barrel control terminal is arranged on the power supply control barrel (36), the control barrel control terminal is connected with the underwater control unit, and the peristaltic pump (14) and the circulating pump (41) are respectively connected with the control barrel control terminal through a control barrel control cable; two ends of the tension spring (11) are respectively connected with the upper cover (19) and the placing disc (10).
2. The long-time-series deep-sea in-situ large scale organism stress culture device according to claim 1, wherein: a pressing block upper end tooth (26) is arranged on the upper cover switch pressing block (25), the switch lower end pressing block is divided into a switch lower end pressing block A (29) and a switch lower end pressing block B (32), the switch lower end pressing block A (29) is fixedly connected to the fixing hoop, the switch lower end pressing block B (32) is rotatably connected with the switch lower end pressing block A (29) through a rotating shaft (33), a torsion spring (34) is sleeved on the rotating shaft (33), and two pressing edges of the torsion spring (34) are respectively abutted to the switch lower end pressing block A (29) and the switch lower end pressing block B (32); and the switch lower end pressing block B (32) is provided with switch lower end teeth (27), and the pressing block upper end teeth (26) on the upper cover switch pressing block (25) are meshed with the switch lower end teeth (27) on the switch lower end pressing block B (32) when the upper cover (19) and the stress culture barrel body (35) are closed.
3. The long-time-series deep-sea in-situ large scale organism stress culture device according to claim 1, wherein: one side of the upper cover (19) is connected with the coercion cultivation barrel body (35) through a damping hinge (4), one end of the damping hinge (4) is fixedly connected to the upper cover (19), and the other end of the damping hinge (4) is fixedly connected to the coercion cultivation barrel body (35).
4. The long-time-series deep-sea in-situ large scale organism stress culture device according to claim 3, wherein: and a connecting line between the center of the projection of one end of the damping hinge (4) on the upper cover (19) and the center of the projection of the upper cover switch pressing block (25) on the upper cover (19) passes through the circle center of the upper cover (19).
5. The long-time-series deep-sea in-situ large scale organism stress culture device according to claim 3, wherein: one end of the damping hinge (4) is connected with a hinge extension plate (6), the lower surface of the hinge extension plate (6) is provided with an upper end fixing ring (5), a lower end fixing ring (13) is arranged on the placing disc (10), and two ends of the tension spring (11) are respectively hooked on the upper end fixing ring (5) and the lower end fixing ring (13).
6. The long-time-series deep-sea in-situ large scale organism stress culture device according to claim 1, wherein: and the upper cover (19) is provided with a handle A (1), a one-way valve (2) and a stop ball valve (3) respectively.
7. The long-time-series deep-sea in-situ large scale organism stress culture device according to claim 1, wherein: the fixed hoop is divided into an upper end fixed hoop (7) and a lower end fixed hoop (8), the switch lower end pressing block is installed on the upper end fixed hoop (7), and a handle B (9) which is obliquely arranged and is convenient for the ROV manipulator to grab is installed on the lower end fixed hoop (8).
8. The long-time-series deep-sea in-situ large scale organism stress culture device according to claim 1, wherein: a sealing gasket (23) is arranged on the inner side of the upper cover (19), and the outer edge (24) of the sealing gasket (23) is smaller than the outer edge of the upper cover (19); when the upper cover (19) is closed and compressed, the sealing gasket (23) is in sealing and abutting joint with the stress culture barrel body (35).
9. The long-time-series deep-sea in-situ large scale organism stress culture device according to claim 1, wherein: the peristaltic pump (14) is arranged on the placing disc (10) through a peristaltic pump fixing frame (12), and the circulating pump (41) is fixed on the placing disc (10) through a circulating pump mounting frame (42); the control barrel control terminal on the power supply control barrel (36) is divided into a control barrel control terminal A (37) and a control barrel control terminal B (38), the peristaltic pump (14) is connected with the control barrel control terminal A (37) through a control barrel control cable A (39), and the underwater control unit controls the peristaltic pump (14) to pump the bottom liquid in the sample bag (18) into the stress culture barrel body (35) through the sampling pipe (17) and the injection pipe (15); the circulating pump (41) is connected with a control barrel control terminal B (38) through a control barrel control cable B (40), the underwater control unit controls the circulating pump (41), and external seawater obtained through a circulating water obtaining pipe (45) is pumped into the stress culture barrel body (35) through a circulating water inlet pipe (44).
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| CN202022351802.0U CN213549124U (en) | 2020-10-20 | 2020-10-20 | Long time sequence deep sea in-situ large-scale organism stress culture device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112219780A (en) * | 2020-10-20 | 2021-01-15 | 中国科学院海洋研究所 | Long-time-series deep-sea in-situ large-scale organism stress culture device and use method thereof |
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2020
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112219780A (en) * | 2020-10-20 | 2021-01-15 | 中国科学院海洋研究所 | Long-time-series deep-sea in-situ large-scale organism stress culture device and use method thereof |
| CN112219780B (en) * | 2020-10-20 | 2023-09-12 | 中国科学院海洋研究所 | Long-time-sequence deep-sea in-situ large-scale biological stress culture device and use method thereof |
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