CN114342862B - Deep sea large-scale organism high-pressure transfer device and transfer method thereof - Google Patents
Deep sea large-scale organism high-pressure transfer device and transfer method thereof Download PDFInfo
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- CN114342862B CN114342862B CN202210083236.0A CN202210083236A CN114342862B CN 114342862 B CN114342862 B CN 114342862B CN 202210083236 A CN202210083236 A CN 202210083236A CN 114342862 B CN114342862 B CN 114342862B
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Abstract
The invention belongs to the field of simulation experiment research of high-pressure culture of large organisms, in particular to a high-pressure transfer device and a transfer method of the large organisms in deep sea. In the high-pressure transfer process, the invention solves the problem of high-pressure transfer technology of large-scale organisms under high pressure through a transfer device; the material is made of stainless steel with strong corrosion resistance.
Description
Technical Field
The invention belongs to the field of simulation experiment research of high-pressure culture of large organisms, and particularly relates to a deep-sea large organism high-pressure transfer device and a transfer method thereof.
Background
The deep sea has special environments such as deep sea plain, sea mountain, hot liquid, cold spring (cold infiltration) and the like, and a unique ecological system and a unique life process are inoculated. Pressure-resistant and pressure-philic large organisms and microorganisms are important groups in the deep sea ecosystem. With the progress of pressure-resistant detection technology, more researches have been attempted in recent years on related large-scale organisms and microorganisms through temperature-controlled and pressure-controlled simulation experiments and on-site culture experiments. However, the shore-based simulation platform under the high-pressure condition is limited by insufficient construction and still is in a starting stage in the home and abroad; particularly, the lack of large-scale biological high-pressure experimental equipment can not be used for describing and verifying the high-pressure state of the physiological and biochemical process, so that only short-term normal-pressure storage and maintenance of large-scale living things can be realized, and analysis of related processes is still easily affected by pressure, so that the in-situ state and process mechanism of deep sea can not be truly reflected.
The portable high-pressure microorganism transferring and culturing device disclosed in 12/17/2014 and having the authorized bulletin number of CN204022810U, the non-pressure-releasing continuous high-pressure microorganism culturing device disclosed in 15/3/15/2017 and having the authorized bulletin number of CN206014873U, the static electric control high-temperature high-pressure microorganism culturing device disclosed in 24/12/2014 and having the authorized bulletin number of CN204039411U, the deep sea cold spring simulation and low-temperature high-pressure microorganism culturing system disclosed in 29/2014 and having the publication number of CN103540521A, the microorganism culturing reaction system disclosed in 11/19/2014 and having the authorized bulletin number of CN203947097U and the like are all pure culturing devices for microorganisms under the high-pressure system, particularly the microorganisms living in the deep sea environment under the simulated high-pressure condition, and the devices related to large-scale organisms are very few. Meanwhile, the existing culture equipment cannot sample large-scale organisms under the condition of pressure maintaining after high-pressure operation.
Disclosure of Invention
Aiming at the bottleneck problem of the current high-pressure large-scale biological culture, the invention aims to provide a deep-sea large-scale biological high-pressure transfer device and a use method thereof.
The aim of the invention is realized by the following technical scheme:
the transfer device comprises a lifting oil cylinder, a high-pressure cabin body, a cold water domain cabin, a rotating tray, a transfer channel, a ball valve, a transfer high-pressure cabin, a fixed extrusion combined pipe, a pushing oil cylinder and an integral frame, wherein the cold water domain cabin for refrigerating seawater in the high-pressure cabin body is arranged outside the high-pressure cabin body, the high-pressure cabin body and the cold water domain cabin are both arranged on the integral frame, the lifting oil cylinder arranged on the integral frame is arranged above the high-pressure cabin body, a lifting oil cylinder piston rod of the lifting oil cylinder is connected with a high-pressure cabin upper cover, and the high-pressure cabin upper cover is tightly sealed with the upper edge of the high-pressure cabin through the lifting oil cylinder; the high-pressure cabin is characterized in that a rotatable rotating tray is arranged in the high-pressure cabin body, a rotating through hole is formed in the rotating tray, a through hole is formed in the bottom of the high-pressure cabin body, an active rotating shaft and a shielding disc rotating shaft are respectively and rotatably arranged on the rotating tray, the active rotating shaft is connected with the rotating tray through a transmission mechanism, and one end of the shielding disc rotating shaft, which is positioned in the high-pressure cabin body, is connected with a through hole shielding disc for controlling a rotating through hole switch; the upper end of the transfer channel is communicated with the hyperbaric chamber main body, the lower end of the transfer channel is communicated with the transfer hyperbaric chamber, and the transfer channel is provided with a ball valve of a control switch; the inside of the transferring high-pressure cabin is provided with a fixed cabin, the fixed cabin is divided into a closed squeezing cabin and a fixed cabin water cabin communicated with the inside of the transferring high-pressure cabin through a squeezing piston, seawater in the squeezing cabin can flow into the transferring high-pressure cabin only in one direction, the inside of the transferring high-pressure cabin is provided with a fixed liquid bag, and fixed liquid in the fixed liquid bag can flow into the squeezing cabin only in one direction; the transfer high-pressure cabin is provided with a fixed extrusion combined pipe, the pushing oil cylinder is arranged on the integral frame, and the output end of the pushing oil cylinder is connected with the extrusion piston through a water cabin rod penetrating through the fixed extrusion combined pipe.
Wherein: the integral frame is provided with a hoop oil cylinder, a hoop pushing rod of the hoop oil cylinder is connected with a high-pressure cabin hoop, and the upper cover of the high-pressure cabin is tightly clamped and sealed with the upper edge of the high-pressure cabin through the high-pressure cabin hoop.
The front end of the transferring high-pressure cabin is provided with a transferring high-pressure cabin front cover, a front cover handle is arranged on the transferring high-pressure cabin front cover, a left hoop and a right hoop are respectively arranged on the left side and the right side of the transferring high-pressure cabin front cover, and the left hoop and the right hoop are respectively driven to open and close by a cover closing oil cylinder arranged on the integral frame.
The pressure gauge for measuring the pressure state in the pressure cabin main body is arranged in the upper cover of the pressure cabin, and the cameras for observing the action conditions of the large organisms in the pressure cabin main body, the rotating tray and the through hole shielding tray are arranged in the upper cover of the pressure cabin.
The driving rotating shaft and the shielding disc rotating shaft are through cabin shafts, namely, the upper ends of the driving rotating shaft and the shielding disc rotating shaft are located in the hyperbaric cabin main body, the lower ends of the driving rotating shaft and the shielding disc rotating shaft are located outside the hyperbaric cabin main body, and the through hole shielding disc is located between the rotating tray and the bottom of the hyperbaric cabin main body.
The transmission mechanism is positioned in the hyperbaric chamber main body and comprises a driving gear and a meshing gear, the driving gear is connected to the upper end of the driving rotating shaft, the meshing gear is linked with the rotating tray and is meshed with the driving gear for transmission, the lower end of the driving rotating shaft is rotated, and the rotating tray is driven to rotate through the meshed transmission of the driving gear and the meshing gear; the upper surface of the rotating tray is uniformly provided with a plurality of rotating check blocks along the circumferential direction.
The transfer channel comprises a transfer channel upper end, a ball valve and a transfer channel lower end which are sequentially connected through threads, a transfer channel hole at the upper end of the transfer channel is communicated with a through hole at the bottom of the hyperbaric chamber main body, and a transfer channel hole at the lower end of the transfer channel is communicated with the fixed cabin through a transfer cabin body falling channel on the transfer hyperbaric chamber.
The front end of the fixed cabin is a fixed cabin front cover, two interfaces are arranged on the fixed cabin front cover, one interface is connected with the fixed liquid bag through a one-way valve A, the other interface is connected with a one-way valve B, the flowing direction of the one-way valve A is that the fixed liquid bag flows to the extrusion cabin, and the flowing direction of the one-way valve B is that the extrusion cabin flows to the inside of the transfer hyperbaric cabin; the rear end of the fixed cabin is a fixed cabin rear cover connected with the rear end of the transferring hyperbaric cabin.
The fixed extrusion combined pipe comprises a balance water tank, a seawater balance pipe, an isolation tank and a pushing oil cylinder, wherein the pushing oil cylinder is arranged on the integral frame through an oil cylinder supporting frame, one end of the isolation tank is connected with the pushing oil cylinder, the other end of the isolation tank is connected with one end of the balance water tank, the other end of the balance water tank is connected with a transfer hyperbaric chamber, water tank rods respectively penetrate through the isolation tank and the balance water tank, and piston seals are arranged on the parts of the water tank rods inside the isolation tank and the balance water tank; the balance water tank is internally divided into a storage water tank and an air tank communicated with the outside through a piston seal, one end of the seawater balance pipe is communicated with the storage water tank, and the other end of the seawater balance pipe is communicated with the inside of the transfer high-pressure cabin.
The volume of the storage water tank is always equal to the volume of the water tank rod passing through the storage water tank, namely, the seawater discharged from the fixed tank water tank to the transfer high-pressure tank by the fixed tank water tank enters the fixed tank water tank and returns to the storage water tank through the seawater balance pipe.
The inside of the isolation cabin is divided into two parts by a piston seal, and each part is provided with an isolation cabin flushing port; the pushing oil cylinder is provided with two oil ports, an oil cylinder piston rod of the pushing oil cylinder is connected with a water tank rod or is the same rod, and the part of the water tank rod in the fixed tank water tank is a piston rod connected with an extrusion piston; the pushing oil cylinder is positioned on one side of the oil cylinder supporting frame, the oil cylinder piston rod is provided with a fixing bolt positioned on the other side of the oil cylinder supporting frame, and the front-back movement range of the piston rod is limited by adjusting the position of the fixing bolt on the oil cylinder piston rod.
The invention relates to a transfer method of a deep-sea large-scale biological high-pressure transfer device, which comprises the following steps:
step one, cleaning a deep sea large-scale biological high-pressure transfer device;
step two, the hoop oil cylinder acts, the hoop of the high-pressure cabin is retracted through the hoop pushing rod, the lifting oil cylinder acts, the piston rod of the lifting oil cylinder is retracted, the upper cover of the high-pressure cabin is opened, the rotating shaft of the shielding disc is rotated, and then the rotating through hole is opened through the through hole shielding disc; adding seawater into the hyperbaric chamber main body, refrigerating the cold water domain cabin by using a refrigerator, refrigerating the water body in the hyperbaric chamber main body by using a heat conduction principle, rotating the rotating shaft of the shielding disc, further closing the rotating through hole by the shielding disc with the through hole, and adding deep-sea macroorganisms into the hyperbaric chamber main body, wherein the deep-sea macroorganisms fall on the rotating tray;
step three, the lifting oil cylinder acts reversely, a piston rod of the lifting oil cylinder is pressed down, the upper cover of the high-pressure cabin is closed, the hoop oil cylinder acts reversely, the hoop of the high-pressure cabin is pressed forwards, and the upper cover of the high-pressure cabin and the upper edge of the high-pressure cabin are locked; the booster pump is utilized to carry out high-pressure pressurization treatment on the high-pressure cabin main body;
step four, after the set time of the deep sea large-scale living in the hyperbaric chamber main body, preparing to perform high-pressure transfer action;
fifthly, the camera is used for internal observation, the rotating shaft of the shielding disc is rotated, and then the rotating through hole is opened through the through hole shielding disc; lifting a ball valve handle outside the ball valve, opening the ball valve, and communicating the high-pressure cabin main body, the upper end of the transfer channel, the ball valve, the lower end of the transfer channel and the transfer high-pressure cabin; the driving rotating shaft is rotated, and the rotating tray is driven to rotate through the transmission mechanism, so that the rotating through hole is communicated with the through hole at the bottom of the hyperbaric chamber main body and the transferring channel hole; the deep-sea macroorganisms on the rotating tray are pushed by the rotating check to reach the rotating through holes, and finally fall into the extrusion cabin for transferring the hyperbaric chamber due to gravity;
step six, oil is fed into an oil port at the rear end of a pushing oil cylinder in the fixed extrusion combined pipe to form forward movement, the water tank rod drives the piston rod and the extrusion piston to advance to extrude the deep-sea large organisms falling into the extrusion chamber, the water body of the extrusion chamber is discharged along with the one-way valve B, after the deep-sea large organisms are extruded and crushed, the pushing oil cylinder reversely moves, the water tank rod drives the piston rod and the extrusion piston to suck back, and fixing liquid flows into the extrusion chamber through the one-way valve A and the fixing liquid bag to fix the deep-sea large organisms;
step seven, a ball valve handle outside the ball valve is pulled down, the ball valve is closed, the pressure inside the hyperbaric chamber main body is guaranteed to be unchanged, the transferring hyperbaric chamber is decompressed, the cover closing oil cylinder acts, the right hoop and the left hoop are opened, the transferring hyperbaric chamber front cover is opened, and the fixed deep sea large organism is taken out;
step eight, closing the front cover of the transferring high-pressure cabin, reversely acting the cover closing oil cylinder, closing the right hoop and the left hoop, pressurizing the transferring high-pressure cabin by using a booster pump to reach the internal pressure of the high-pressure cabin body, lifting a ball valve handle outside the ball valve, and opening the ball valve;
and continuously repeating the steps five to eight to finish the cultivation and the pressurized transfer of the deep sea large organisms.
The invention has the advantages and positive effects that:
in the high-pressure transfer process, the invention solves the problem of high-pressure transfer technology of large-scale organisms under high pressure through a transfer device; the material has strong corrosion resistance and low cost.
Drawings
FIG. 1 is a front elevational view of the transfer device of the present invention;
FIG. 2 is a left side view of the transfer device of the present invention;
FIG. 3 is a schematic view of the structure of FIG. 2 with the integral frame removed;
FIG. 4 is a schematic view of the structure of FIG. 1 with the integral frame, the hyperbaric chamber body, and the cold water chamber removed;
FIG. 5 is a schematic view showing the structure of the inside and the lower part of the hyperbaric chamber body in FIG. 3;
FIG. 6 is a schematic view showing the structure of the inside of the transferring hyperbaric chamber of FIG. 5;
FIG. 7 is a schematic perspective view of a transfer tray, a pod shaft, a transfer passage and a ball valve in the transfer device of the present invention;
FIG. 8 is a second perspective view of a transfer tray, a pod shaft, a transfer channel and a ball valve in the transfer device of the present invention;
wherein: 1 is a lifting oil cylinder, 2 is a lifting oil cylinder piston rod, 3 is a high-pressure chamber upper cover, 4 is a high-pressure chamber upper edge, 5 is a high-pressure chamber main body, 6 is a cold water domain chamber, 7 is a transfer channel upper end, 8 is a ball valve, 9 is a ball valve handle, 10 is a guide rail, 11 is a transfer high-pressure chamber, 12 is a transfer high-pressure chamber front cover, 13 is a front cover handle, 14 is a balance water chamber, 15 is a sea water balance pipe, 16 is an isolation chamber, 17 is an isolation chamber flushing port, 18 is a pushing oil cylinder, 19 is an integral frame, 20 is a support base, 21 is a base, 22 is a closing oil cylinder, 23 is a right hoop, 24 is a left hoop, 25 is an oil port, 26 is an oil cylinder support frame, 27 is a pressure gauge, 28 is a camera, 29 is a rotating tray, 30 is a driving gear, 31 is a through hole shielding disc, 32 is a meshing gear, 33 is a driving rotating shaft, 34 is a shielding disc rotating shaft, 35 is a rotating through hole, 36 is a rotating grid, 37 is a transferring channel hole, 38 is a transferring cabin dropping channel, 39 is a squeezing cabin, 40 is a fixed liquid bag, 41 is a one-way valve A,42 is a single-way valve B,43 is a squeezing piston, 44 is a piston rod, 45 is a piston seal, 46 is a water cabin pole, 47 is a transferring channel lower end, 48 is a fixed cabin front cover, 49 is a fixed cabin rear end cover, 50 is a high-pressure cabin staple bolt, 51 is a staple bolt propelling pole, 52 is a staple bolt oil cylinder, 53 is a transferring high-pressure cabin fixed frame, 54 is a fixed cabin water cabin, 55 is a storage water cabin, 56 is a balance water cabin shell, 57 is a fixed cabin, 58 is a fixed bolt, and 59 is a fixed squeezing combined pipe.
Detailed Description
The invention is described in further detail below with reference to the accompanying drawings.
As shown in fig. 1 to 7, the transfer device of the invention comprises a lifting oil cylinder 1, a hyperbaric chamber main body 5, a cold water domain cabin 6, a rotating tray 29, a transfer channel, a ball valve 8, a transfer hyperbaric chamber 11, a pushing oil cylinder 18, a fixed extrusion combined pipe 59, a hoop oil cylinder 52, a hoop pushing rod 51, a capping oil cylinder 22, a left hoop 24, a right hoop 23 and an integral frame 19, wherein the hyperbaric chamber main body 5 is used as a hyperbaric culture chamber of a large-scale deep sea organism, and the transfer hyperbaric chamber 11 is used as a transfer acquisition chamber of a biological sample; the outside of the high-pressure cabin body 5 is provided with a cold water area cabin 6, the cold water area cabin 6 is used for refrigerating the high-pressure cabin body 5, the water body inside the high-pressure cabin body 5 is refrigerated by utilizing the heat conduction principle, the temperature control of the deep-sea large-scale organisms is completed, the lower ends of the high-pressure cabin body 5 and the cold water area cabin 6 are both arranged on a support base 20, and the support base 20 is welded on an integral frame 19; a lifting oil cylinder 1 is arranged above the high-pressure cabin main body 5, the cylinder body of the lifting oil cylinder 1 is welded on the integral frame 19, a lifting oil cylinder piston rod 2 of the lifting oil cylinder 1 is connected with a high-pressure cabin upper cover 3, the high-pressure cabin upper cover 3 is tightly pressed with the upper edge 4 of the high-pressure cabin through the lifting oil cylinder 1, and high-pressure sealing is carried out by utilizing axial sealing; the two sides of the outer part of the upper cover 3 of the high-pressure cabin and the upper edge 4 of the high-pressure cabin are symmetrically provided with anchor ear oil cylinders 52, the cylinder bodies of the anchor ear oil cylinders 52 are welded on the integral frame 19, anchor ear pushing rods 51 of the anchor ear oil cylinders 52 are connected with the anchor ear 50 of the high-pressure cabin, and the upper cover 3 of the high-pressure cabin and the upper edge 4 of the high-pressure cabin are tightly clamped and sealed through the anchor ear 50 of the high-pressure cabin; the inside of the hyperbaric chamber main body 5 is provided with a rotatable rotating tray 29, the rotating tray 29 is provided with a rotating through hole 35, the bottom of the hyperbaric chamber main body 5 is provided with a through hole, and is respectively provided with a driving rotating shaft 33 and a shielding disc rotating shaft 34 in a rotating way, the driving rotating shaft 33 is connected with the rotating tray 29 through a transmission mechanism, and one end of the shielding disc rotating shaft 34 positioned in the hyperbaric chamber main body 5 is connected with a through hole shielding disc 31 for controlling the opening and closing of the rotating through hole 35; the upper end of the transfer channel is communicated with the hyperbaric chamber main body 5, the lower end of the transfer channel is communicated with the transfer hyperbaric chamber 11, and the transfer channel is provided with a ball valve 8 of a control switch; the transferring hyperbaric chamber 11 is internally provided with a fixed chamber 57, the fixed chamber 57 is divided into a closed squeezing chamber 39 and a fixed chamber water chamber 54 communicated with the inside of the transferring hyperbaric chamber 11 through a squeezing piston 43, the seawater in the squeezing chamber 39 can flow into the transferring hyperbaric chamber 11 only in one direction, the transferring hyperbaric chamber 11 is internally provided with a fixed liquid bag 40, and the fixed liquid in the fixed liquid bag 40 can flow into the squeezing chamber 39 only in one direction; the fixed extrusion combined pipe 59 is installed on the transferring hyperbaric chamber 11, the pushing cylinder 18 is installed on the integral frame 19, and the output end of the pushing cylinder 18 is connected with the extrusion piston 43 through the water tank rod 46 passing through the fixed extrusion combined pipe 59.
The front end of the transferring high-pressure cabin 11 is a transferring high-pressure cabin front cover 12, a front cover handle 13 is welded on the transferring high-pressure cabin front cover 12, a left hoop 24 and a right hoop 23 are respectively arranged on the left side and the right side of the transferring high-pressure cabin front cover 12, and the left hoop 24 and the right hoop 23 are respectively driven to open and close by a cover closing oil cylinder 22; the base 21 at the bottom of the integral frame 19 is welded with a transferring high-pressure chamber fixing frame 53, cylinder bodies of cover closing cylinders 22 at the left side and the right side of the transferring high-pressure chamber front cover 12 are respectively welded on the transferring high-pressure chamber fixing frame 53, two parallel guide rails 10 are arranged on the transferring high-pressure chamber fixing frame 53, a left hoop 24 and a right hoop 23 are respectively connected with the two guide rails 10 in a sliding manner, and the cover closing cylinders 22 play a role in supporting and guiding in the process of driving the left hoop 24 and the right hoop 23 to open and close.
The transfer channel of this embodiment includes transfer channel upper end 7, ball valve 8 and transfer channel lower extreme 47 that loop through threaded connection, and transfer channel hole 37 of transfer channel upper end 7 is linked together with the through-hole of hyperbaric chamber main part 5 bottom, and transfer channel hole 37 of transfer channel lower extreme 47 is linked together with fixed cabin 57 through transfer cabin body drop channel 38 on the transfer hyperbaric chamber 11. The ball valve 8 is externally connected with a ball valve handle 9. The high-pressure cabin body 5, the transferring high-pressure cabin 11, the middle transferring channel upper end 7, the ball valve 8 and the transferring channel lower end 47 are all parts bearing high pressure and are communicated, the ball valve 8 can be used for closing the connection between the high-pressure cabin body 5 and the transferring high-pressure cabin 11, the high pressure is completed by injecting high-pressure water body through an external booster pump, and the pressure in the high-pressure cabin body 5 and the transferring high-pressure cabin 11 can reach 20MPa.
The pressure gauge 27 and the camera 28 are respectively installed in the upper cover 3 of the hyperbaric chamber in this embodiment, the pressure gauge 27 is used for measuring the pressure state in the main body 5 of the hyperbaric chamber, the camera 28 is used for observing the action conditions of the large organism in the main body 5 of the hyperbaric chamber, the rotating tray 29 and the through hole shielding plate 31, and the camera 28 is placed above the through hole at the bottom of the main body 5 of the hyperbaric chamber.
The driving rotation shaft 33 and the shielding plate rotation shaft 34 of the present embodiment are all cabin passing shafts, that is, the upper ends of the driving rotation shaft 33 and the shielding plate rotation shaft 34 are all located in the hyperbaric chamber main body 5, the lower ends of the driving rotation shaft 33 and the shielding plate rotation shaft 34 are all located outside the hyperbaric chamber main body 5, and the through hole shielding plate 31 is located between the rotation tray 29 and the bottom of the hyperbaric chamber main body 5. The transmission mechanism of the embodiment is positioned in the hyperbaric chamber main body 5, the transmission mechanism comprises a driving gear 30 and a meshing gear 32, the center of the driving gear 30 is connected to the upper end of a driving rotating shaft 33, the center of the meshing gear 32 is linked with the center of a rotating tray 29 and is meshed with the driving gear 30 for transmission, the lower end of the driving rotating shaft 33 is manually rotated, and the rotating tray 29 is driven to rotate through the meshed transmission of the driving gear 30 and the meshing gear 32; the upper surface of the rotary tray 29 is provided with a plurality of rotary stoppers 36 uniformly in the circumferential direction. When the shutter disk rotating shaft 34 is manually rotated, the through hole shutter disk 31 is driven to rotate, the rotating through hole 35 is blocked or opened, and when the rotating through hole 35 is opened, the hyperbaric chamber main body 5, the rotating through hole 35, the transfer passage hole 37, the transfer passage upper end 7, the ball valve 8, the transfer passage lower end 47, the transfer chamber drop passage 38 and the extrusion chamber 39 are in a passage state.
The upper end of the transferring hyperbaric chamber 11 is a transferring chamber falling channel 38, and the inside of the middle main chamber part is a fixed chamber 57; the front end of the fixed cabin 57 is provided with a fixed cabin front cover 48, the fixed cabin front cover 48 is provided with two interfaces, one interface is connected with the fixed liquid bag 40 through a one-way valve A41, the other interface is connected with a one-way valve B42, the flow direction of the one-way valve A41 is that the fixed liquid bag 40 flows to the extrusion cabin 39, and the flow direction of the one-way valve B42 is that the extrusion cabin 39 flows to the inside of the transfer hyperbaric cabin 11; the rear end of the fixed compartment 57 is a fixed compartment rear cover 49 connected to the rear end of the transfer pressure compartment 11.
The fixed extrusion combined pipe 59 of the embodiment comprises a balance water tank 14, a seawater balance pipe 15, an isolation tank 16 and a pushing oil cylinder 18, wherein the pushing oil cylinder 18 is fixed on the integral frame 19 through an oil cylinder supporting frame 26, one end of the isolation tank 16 is connected with the pushing oil cylinder 18, the other end of the isolation tank is connected with one end of the balance water tank 14, the other end of the balance water tank 14 is connected with the rear end of the transfer hyperbaric chamber 11, a water tank rod 46 respectively passes through the isolation tank 16 and the balance water tank 14, and piston seals 45 are arranged on the parts of the water tank rod 46 inside the isolation tank 16 and the balance water tank 14; the balance water tank 14 is divided into a storage water tank 55 and an air tank communicated with the outside through a piston seal 45, one end of the seawater balance pipe 15 is communicated with the storage water tank 55, and the other end is communicated with the inside of the transferring high-pressure cabin 11.
The storage water tank 55 of this embodiment is wrapped by the balance water tank housing 56, the storage water tank 55 is used for storing high-pressure water, the volume of the storage water tank 55 is always equal to the volume of the water tank rod 46 passing through the storage water tank 55, that is, the seawater discharged from the fixed tank water tank 54 into the transferring high-pressure chamber 11 by the fixed tank water tank 54 returns to the storage water tank 55 through the seawater balance pipe 15, so as to realize the internal pressure maintaining of the transferring high-pressure chamber 11.
The inside of the isolation cabin 16 of the embodiment is divided into two parts by a piston seal 45, and each part is provided with an isolation cabin flushing port 17; the isolation cabin 16 is an isolation part between the balance water cabin 14 and the pushing oil cylinder 18, prevents the oil from being mixed with the water in the high-pressure cabin main body 5, and has long time, and fresh water needs to be filled into the isolation cabin flushing port 17 for water flushing. The pushing oil cylinder 18 is provided with two oil ports 25, an oil cylinder piston rod of the pushing oil cylinder 18 is connected with the water tank rod 46 or is the same rod, and the part of the water tank rod 46 in the fixed tank water tank 54 is a piston rod 44 connected with the extrusion piston 43 (the piston rod 44 and the water tank rod 46 in the embodiment are the same stainless steel tube); the pushing cylinder 18 is positioned on one side of the cylinder support frame 26, a fixing bolt 58 positioned on the other side of the cylinder support frame 26 is arranged on a cylinder piston rod, and the front-back movement range of the piston rod 44 is limited by adjusting the position of the fixing bolt 58 on the cylinder piston rod.
The invention relates to a transfer method of a deep-sea large-scale biological high-pressure transfer device, which comprises the following steps:
step one, cleaning a deep sea large-scale biological high-pressure transfer device;
step two, the hoop oil cylinder 52 acts, the high-pressure cabin hoop 50 is retracted through the hoop pushing rod 51, the lifting oil cylinder 1 acts, the lifting oil cylinder piston rod 2 is retracted, the high-pressure cabin upper cover 3 is opened, the shielding disc rotating shaft 34 is rotated, and the rotating through hole 35 is opened through the through hole shielding disc 31; adding seawater into the hyperbaric chamber main body 5, refrigerating the water area cabin 6 by using a refrigerating machine, refrigerating the water body in the hyperbaric chamber main body 5 (such as 3.2 ℃) by using a heat conduction principle, rotating the shielding disc rotating shaft 34, further closing the rotating through hole 35 by the through hole shielding disc 31, and then adding deep-sea macroorganisms into the hyperbaric chamber main body 5, wherein the deep-sea macroorganisms fall on the rotating tray 29;
step three, the lifting oil cylinder 1 acts reversely, the piston rod 2 of the lifting oil cylinder is pressed down, the upper cover 3 of the high-pressure cabin is closed, the hoop oil cylinder 52 acts reversely, and the front pressure cabin hoop 50 locks the upper cover 3 of the high-pressure cabin and the upper edge 4 of the high-pressure cabin; the booster pump is used for carrying out high-pressure pressurization treatment on the high-pressure cabin main body 5;
step four, after the deep sea large-scale living time (such as one week) in the hyperbaric chamber main body 5, preparing to perform high-pressure transfer action;
fifthly, the camera 28 is used for internal observation, the rotating shaft 34 of the shielding disc is rotated, and then the rotating through hole 35 is opened through the through hole shielding disc 31; a ball valve handle 9 outside the lifting ball valve 8 is used for opening the ball valve 8, and the high-pressure cabin body 5, the upper end 7 of the transfer passage, the ball valve 8, the lower end 47 of the transfer passage and the transfer high-pressure cabin 11 are communicated; the driving rotation shaft 33 is rotated to drive the driving gear 30 and the meshing gear 32 to rotate, and finally the rotation tray 29 is driven to rotate, so that the rotation through hole 35 is communicated with the through hole at the bottom of the hyperbaric chamber main body 5 and the transfer passage hole 37; the deep-sea macroorganisms on the rotating tray 29 are pushed by the rotating barrier 36 to the rotating through hole 35, because gravity finally falls into the pressing compartment 39 of the transferring hyperbaric chamber 11;
step six, the oil port 25 at the rear end of the pushing oil cylinder 18 in the fixed extrusion combined pipe 59 is filled with oil to form forward movement, the water tank rod 46 drives the piston rod 44 and the extrusion piston 43 to advance, the deep-sea creatures falling into the extrusion chamber 39 are extruded, the water body of the extrusion chamber 39 is discharged along with the check valve B42, when the deep-sea creatures are extruded and crushed (the extrusion degree can be adjusted according to the position of the fixing bolt 58 on the oil cylinder piston rod), the pushing oil cylinder 18 reversely moves, the water tank rod 46 drives the piston rod 44 and the extrusion piston 43 to suck back, and the fixing liquid flows into the extrusion chamber 39 through the check valve A41 and the fixing liquid bag 40 to fix the deep-sea creatures;
step seven, the ball valve handle 9 outside the ball valve 8 is pulled down, the ball valve 8 is closed, the pressure inside the high-pressure cabin main body 5 is guaranteed to be unchanged, the pressure of the transferring high-pressure cabin 11 is relieved, the cover closing oil cylinder 22 acts, the right hoop 23 and the left hoop 24 are opened, the transferring high-pressure cabin front cover 12 is opened, and the fixed deep sea large-scale creatures are taken out;
step eight, closing the front cover 12 of the transferring high-pressure cabin, reversely acting the cover closing oil cylinder 22, closing the right hoop 23 and the left hoop 24, pressurizing the transferring high-pressure cabin 11 by utilizing a booster pump to reach the internal pressure of the high-pressure cabin main body 5, lifting the ball valve handle 9 outside the ball valve 8, and opening the ball valve 8;
and continuously repeating the steps five to eight to finish the cultivation and the pressurized transfer of the deep sea large organisms.
The invention belongs to the field of high-pressure culture simulation experiment research of large-scale organisms in deep sea, which is suitable for the large-scale organisms with weak motility, such as mussels and calcium shrimp, and solves the problem of high-pressure transfer technology of the large-scale organisms under high pressure through a rotating device in the high-pressure transfer process; the material has strong corrosion resistance and low cost.
Claims (11)
1. The utility model provides a large-scale biological high pressure transfer device in deep sea which characterized in that: the high-pressure cabin comprises a lifting oil cylinder (1), a high-pressure cabin body (5), a cold water domain cabin (6), a rotating tray (29), a transfer channel, a ball valve (8), a transfer high-pressure cabin (11), a fixed extrusion combined pipe (59), a pushing oil cylinder (18) and an integral frame (19), wherein the cold water domain cabin (6) for refrigerating seawater inside the high-pressure cabin body (5) is arranged outside the high-pressure cabin body (5), the high-pressure cabin body (5) and the cold water domain cabin (6) are both arranged on the integral frame (19), the lifting oil cylinder (1) arranged on the integral frame (19) is arranged above the high-pressure cabin body (5), a lifting oil cylinder piston rod (2) of the lifting oil cylinder (1) is connected with a high-pressure cabin upper cover (3), and the high-pressure cabin upper cover (3) is tightly sealed with an upper edge (4) through the lifting oil cylinder (1); the high-pressure cabin is characterized in that a rotatable rotating tray (29) is arranged inside the high-pressure cabin body (5), a rotating through hole (35) is formed in the rotating tray (29), a through hole is formed in the bottom of the high-pressure cabin body (5), a driving rotating shaft (33) and a shielding disc rotating shaft (34) are respectively rotatably arranged at the bottom of the high-pressure cabin body, the driving rotating shaft (33) is connected with the rotating tray (29) through a transmission mechanism, and one end of the shielding disc rotating shaft (34) located in the high-pressure cabin body (5) is connected with a through hole shielding disc (31) for controlling the opening and closing of the rotating through hole (35); the upper end of the transfer channel is communicated with the hyperbaric chamber main body (5), the lower end of the transfer channel is communicated with the transfer hyperbaric chamber (11), and the transfer channel is provided with a ball valve (8) for controlling a switch; the inside of the transferring high-pressure cabin (11) is provided with a fixed cabin (57), the fixed cabin (57) is divided into a closed extruding cabin (39) and a fixed cabin water cabin (54) communicated with the inside of the transferring high-pressure cabin (11) through an extruding piston (43), the seawater in the extruding cabin (39) can flow into the transferring high-pressure cabin (11) only in one direction, the inside of the transferring high-pressure cabin (11) is provided with a fixed liquid bag (40), and the fixed liquid in the fixed liquid bag (40) can flow into the extruding cabin (39) only in one direction; the transfer high-pressure cabin (11) is provided with a fixed extrusion combined pipe (59), the pushing oil cylinder (18) is arranged on the integral frame (19), and the output end of the pushing oil cylinder (18) is connected with the extrusion piston (43) through a water cabin rod (46) penetrating through the fixed extrusion combined pipe (59).
2. The deep sea large-scale biological high pressure transfer device according to claim 1, wherein: the high-pressure cabin is characterized in that a hoop oil cylinder (52) is arranged on the integral frame (19), a hoop pushing rod (51) of the hoop oil cylinder (52) is connected with a high-pressure cabin hoop (50), and the high-pressure cabin upper cover (3) and the high-pressure cabin upper edge (4) are clamped and sealed through the high-pressure cabin hoop (50).
3. The deep sea large-scale biological high pressure transfer device according to claim 1, wherein: the front end of the transferring high-pressure cabin (11) is provided with a transferring high-pressure cabin front cover (12), the transferring high-pressure cabin front cover (12) is provided with a front cover handle (13), the left side and the right side of the transferring high-pressure cabin front cover (12) are respectively provided with a left hoop (24) and a right hoop (23), and the left hoop (24) and the right hoop (23) are respectively driven to open and close by a cover closing oil cylinder (22) arranged on the integral frame (19).
4. The deep sea large-scale biological high pressure transfer device according to claim 1, wherein: the pressure gauge (27) for measuring the internal pressure state of the pressure cabin main body (5) and the camera (28) for observing the action conditions of the large-scale creatures, the rotating tray (29) and the through hole shielding tray (31) in the pressure cabin main body (5) are respectively arranged in the pressure cabin upper cover (3).
5. The deep sea large-scale biological high pressure transfer device according to claim 1, wherein: the driving rotating shaft (33) and the shielding disc rotating shaft (34) are through cabin shafts, namely, the upper ends of the driving rotating shaft (33) and the shielding disc rotating shaft (34) are located in the hyperbaric cabin main body (5), the lower ends of the driving rotating shaft (33) and the shielding disc rotating shaft (34) are located outside the hyperbaric cabin main body (5), and the through hole shielding disc (31) is located between the rotating tray (29) and the bottom of the hyperbaric cabin main body (5).
6. The deep sea large-scale biological high pressure transfer device according to claim 1, wherein: the transmission mechanism is positioned in the hyperbaric chamber main body (5), the transmission mechanism comprises a driving gear (30) and a meshing gear (32), the driving gear (30) is connected to the upper end of a driving rotating shaft (33), the meshing gear (32) is linked with the rotating tray (29) and is meshed with the driving gear (30) for transmission, the lower end of the driving rotating shaft (33) is rotated, and the rotating tray (29) is driven to rotate through the meshed transmission of the driving gear (30) and the meshing gear (32); the upper surface of the rotary tray (29) is uniformly provided with a plurality of rotary screens (36) along the circumferential direction.
7. The deep sea large-scale biological high pressure transfer device according to claim 1, wherein: the transfer channel comprises a transfer channel upper end (7), a ball valve (8) and a transfer channel lower end (47) which are sequentially connected through threads, a transfer channel hole (37) of the transfer channel upper end (7) is communicated with a through hole at the bottom of the hyperbaric chamber main body (5), and the transfer channel hole (37) of the transfer channel lower end (47) is communicated with the fixed chamber (57) through a transfer chamber body falling channel (38) on the transfer hyperbaric chamber (11).
8. The deep sea large-scale biological high pressure transfer device according to claim 1, wherein: the front end of the fixed cabin (57) is provided with a fixed cabin front cover (48), the fixed cabin front cover (48) is provided with two interfaces, one interface is connected with the fixed liquid bag (40) through a one-way valve A (41), the other interface is connected with a one-way valve B (42), the flow direction of the one-way valve A (41) is that the fixed liquid bag (40) flows to the extrusion cabin (39), and the flow direction of the one-way valve B (42) is that the extrusion cabin (39) flows to the inside of the transfer high-pressure cabin (11); the rear end of the fixed cabin (57) is a fixed cabin rear cover (49) connected with the rear end of the transferring hyperbaric cabin (11).
9. The deep sea large-scale biological high pressure transfer device according to claim 1, wherein: the fixed extrusion combined pipe (59) comprises a balance water tank (14), a seawater balance pipe (15), an isolation tank (16) and a pushing oil cylinder (18), wherein the pushing oil cylinder (18) is arranged on the integral frame (19) through an oil cylinder supporting frame (26), one end of the isolation tank (16) is connected with the pushing oil cylinder (18), the other end of the isolation tank is connected with one end of the balance water tank (14), the other end of the balance water tank (14) is connected with a transfer hyperbaric chamber (11), a water tank rod (46) respectively penetrates through the isolation tank (16) and the balance water tank (14), and piston seals (45) are arranged on the parts of the water tank rod (46) inside the isolation tank (16) and the balance water tank (14); the inside of the balance water tank (14) is divided into a storage water tank (55) and an air tank communicated with the outside through a piston seal (45), one end of the seawater balance pipe (15) is communicated with the storage water tank (55), and the other end of the seawater balance pipe is communicated with the inside of the transfer high-pressure cabin (11).
10. The deep sea large-scale biological high pressure transfer device according to claim 9, wherein: the volume of the storage water tank (55) is always equal to the volume of the water tank rod (46) penetrating through the storage water tank (55), namely, the seawater discharged into the transferring high-pressure cabin (11) from the fixed water tank (54) enters the fixed water tank (54) through the seawater balance pipe (15) and returns to the storage water tank (55).
11. The deep sea large-scale biological high pressure transfer device according to claim 9, wherein: the inside of the isolation cabin (16) is divided into two parts by a piston seal (45), and each part is provided with an isolation cabin flushing port (17); the pushing oil cylinder (18) is provided with two oil ports (25), an oil cylinder piston rod of the pushing oil cylinder (18) is connected with a water tank rod (46) or is the same rod, and the part of the water tank rod (46) in the fixed tank water tank (54) is a piston rod (44) connected with an extrusion piston (43); the pushing oil cylinder (18) is positioned on one side of the oil cylinder supporting frame (26), a fixing bolt (58) positioned on the other side of the oil cylinder supporting frame (26) is arranged on the oil cylinder piston rod, and the front-back movement range of the piston rod (44) is limited by adjusting the position of the fixing bolt (58) on the oil cylinder piston rod.
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| CN202210083236.0A CN114342862B (en) | 2022-01-25 | 2022-01-25 | Deep sea large-scale organism high-pressure transfer device and transfer method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP4262530B2 (en) * | 2003-06-18 | 2009-05-13 | エイブル株式会社 | Deep sea biological aquarium |
| CN108990925B (en) * | 2018-08-06 | 2023-07-18 | 湖南科技大学 | Deep sea cold spring micro-organism collection and fidelity storage device and use method thereof |
| CN110012869B (en) * | 2019-04-28 | 2022-01-25 | 中国科学院深海科学与工程研究所 | Deep sea large organism low temperature pressurize sampling device |
| CN111406702A (en) * | 2020-04-20 | 2020-07-14 | 张训东 | Biological transport box in deep sea |
| CN113728987B (en) * | 2021-10-09 | 2022-06-21 | 中国船舶科学研究中心 | From come-up deep sea biological sampling device |
| CN216821333U (en) * | 2022-01-25 | 2022-06-28 | 中国科学院海洋研究所 | High-pressure transfer device for large-scale organisms in deep sea |
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