CN215774952U - Deep sea macrobiology fidelity culture device - Google Patents
Deep sea macrobiology fidelity culture device Download PDFInfo
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- CN215774952U CN215774952U CN202023043470.6U CN202023043470U CN215774952U CN 215774952 U CN215774952 U CN 215774952U CN 202023043470 U CN202023043470 U CN 202023043470U CN 215774952 U CN215774952 U CN 215774952U
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Abstract
The utility model discloses a deep sea macrobiology fidelity culture device which comprises a supporting and adjusting module, a control module, a culture module and a refrigeration module, wherein the supporting and adjusting module is fixedly arranged on a container, the culture module is rotatably arranged on the supporting and adjusting module, the refrigeration module is arranged on the culture module, and the control module is respectively connected with the culture module and the refrigeration module. The deep sea macrobiotic fidelity culture device can monitor the state parameters of the macrobiotic culture process, the adopted computer display interface is simple and visual, and the temperature curve and the pressure curve in the deep sea macrobiotic sampling device can be accurately observed; the temperature and the pressure threshold are set by a computer, the refrigerating power of the refrigerating machine is controlled, the low-temperature requirement is realized, the high-pressure requirement is realized by controlling the pressure pump, and the condition that the environmental pressure and the temperature of the deep-sea macroorganisms are similar to the in-situ pressure and the temperature of the deep-sea macroorganisms can be ensured.
Description
Technical Field
The utility model relates to a benthos culture device, in particular to a deep sea macrobiotic fidelity culture device.
Background
Research results show that the mining of marine biological resources is helpful for searching brand new species and life mechanisms; meanwhile, the marine organisms are used as ideal medicines and industrial material resources, and have potential economic value. If the in-situ culture experiment of the benthos can be carried out, the benthos living body sample can be effectively stored, and more comprehensive data support and basis can be provided for revealing the geochemical cycle mechanism of the deep-sea creature. The patent CN111471576A proposes a deep-sea microorganism in-situ culture device with pressure compensation, which can perform enrichment culture and recovery of microorganisms in deep-sea environment, but only maintains the in-situ pressure of microorganisms, and cannot control the temperature variation of the recovery process. Patent CN111109159A proposes a pressure-maintaining and sampling device for marine macroorganisms, which can transfer the captured macroorganisms completely, but cannot keep the temperature and transfer them, and is not suitable for storing the samples of marine macroorganisms for a long time in fidelity. This will have a great influence on the precise study of the living condition of the organisms in the seabed area, the seabed environment and the like. Therefore, a deep sea macrobiosis fidelity culture device which is simple in structure, convenient to operate and automatic is urgently needed to be developed, so that a high-quality seabed in-situ sample is provided for scientific researches such as deep sea macrobiosis community life evolution and the like.
Disclosure of Invention
In order to solve the technical problems, the utility model provides the deep sea macrobiology fidelity culture device which is simple in structure, convenient to operate and high in automation degree.
The technical scheme for solving the problems is as follows: the utility model provides a macro-organism fidelity culture apparatus in deep sea, is including supporting adjusting module, control module, cultivateing module, refrigeration module, support adjusting module fixed mounting on the container, cultivate the rotatable installation of module on supporting adjusting module, be equipped with the refrigeration module on cultivateing the module, control module is connected with cultivateing module, refrigeration module respectively.
The deep sea macrobiology fidelity culture device comprises a pressure maintaining cylinder and an end cover, wherein the pressure maintaining cylinder is a semi-closed cylinder body with an opening at one end, the opening end of the pressure maintaining cylinder is sealed by the end cover, the outer end face of the end cover is provided with a watertight connector II, the watertight connector II is connected with a control module, the inner end face of the end cover is provided with a watertight connector I connected with the watertight connector II, the watertight connector I is connected with a high-pressure camera, a high-pressure lamp, a pressure sensor and a temperature sensor which are arranged in an inner cavity of the pressure maintaining cylinder, the center of the end cover is provided with a butt joint hole for butt joint with a sampling module and a through hole for macrobiology to pass through, a turning plate sealing mechanism for closing or opening the through hole is arranged in the inner cavity of the pressure maintaining cylinder, a horn mouth is arranged on the turning plate sealing mechanism, and a switch valve for driving the turning plate sealing mechanism is arranged on the end cover; the end cover is provided with a plurality of high-pressure pipe connecting holes, and the inner cavity of the pressure maintaining cylinder is connected with a pressure gauge, an overflow valve and a high-pressure valve which are arranged on the outer end surface of the end cover through the high-pressure pipe connecting holes.
Above-mentioned macrobiology fidelity culture apparatus in deep sea, be equipped with the automatic device of delivering of bait on the end cover, the automatic device of delivering of bait is through high-pressure tube connecting hole and pressurize a section of thick bamboo inner chamber intercommunication.
According to the deep sea macrobiotic fidelity culture device, the drainage device is arranged at the bottom of the pressure maintaining cylinder and comprises a high-pressure ball valve, a drainage cylinder and a drainage valve, one end of the high-pressure ball valve is communicated with the inner cavity of the pressure maintaining cylinder, the other end of the high-pressure ball valve is communicated with one end of the drainage cylinder, and the other end of the drainage cylinder is connected with the drainage valve; the bottom of the inner cavity of the pressure maintaining cylinder is provided with a filter plate for limiting the size of the deep sea benthos, and the filter plate is provided with filter holes.
Above-mentioned macro-biological fidelity culture apparatus in deep sea, the refrigeration module includes condenser pipe, refrigerator, be equipped with the condenser pipe in the pressurize section of thick bamboo inner chamber, pressurize section of thick bamboo side is equipped with the connecting hole that the condenser pipe goes out, the entry, refrigerator one end and condenser pipe entry linkage, the other end and condenser pipe exit linkage, the refrigerator is connected with control module.
In the deep sea macrobiotic fidelity culture device, the control module comprises a computer and a controller; the computer is connected with the controller, and the controller is connected with the watertight connector II; the display of the computer comprises a temperature curve, a pressure curve, a real-time temperature and pressure data display window, and the control buttons of the computer comprise a temperature threshold setting button, a pressure threshold setting button and a data real-time storage button.
Above-mentioned grand living beings fidelity culture apparatus in deep sea, support adjusting module and include base, support frame, motor, driven gear, the pedestal mounting sets up two support frames on the container, bilateral symmetry on the base, pressurize section of thick bamboo side symmetry is equipped with two shoulders, all installs the axis of rotation on every shoulder, and axis of rotation one end is installed on the shoulder that corresponds the side, and the axis of rotation other end passes through the bearing to be installed in the bearing frame that is located the support frame top that corresponds the side, and fixed being equipped with the driving gear in one of them axis of rotation, driving gear and the driven gear meshing that is located its below, driven gear pass through the transmission shaft and link to each other with the motor of fixed mounting on the support frame, and the motor is connected with the controller.
In the deep sea macro-organism fidelity culture device, the pressure maintaining cylinder and the drainage cylinder are made of 316 stainless steel, and the upper limit of pressure borne by the pressure maintaining cylinder, the drainage cylinder, the high-pressure camera, the high-pressure lamp, the pressure sensor, the temperature sensor, the overflow valve and the high-pressure valve is greater than 110 MPa.
According to the deep sea macro organism fidelity culture device, the rotating speed of the motor is 1450r/min, the number of teeth of the driving gear is 24, and the number of teeth of the driven gear is 72.
The utility model has the beneficial effects that:
1. the deep sea macrobiotic fidelity culture device can monitor the state parameters of the macrobiotic culture process, the adopted computer display interface is simple and visual, and the temperature curve and the pressure curve in the deep sea macrobiotic sampling device can be accurately observed.
2. The deep sea macrobiotic fidelity culture device of the utility model sets temperature and pressure threshold values through a computer, controls the refrigeration power of the refrigerator, realizes the requirement of low temperature, controls the pressure pump to realize the requirement of high pressure, and can ensure that the environmental pressure and temperature of the deep sea macrobiotic are similar to the in-situ pressure and temperature.
3. The deep sea macrobiotic fidelity culture device can realize long-time culture of macroorganisms, can discharge water and macrobiotic excrement in the culture device by designing the drainage device, and can regularly replace the water in the culture device, so that no pressure drop is kept in the whole process.
Drawings
Fig. 1 is a schematic perspective view of the present invention.
Fig. 2 is a right side view of the three-dimensional structure of the present invention.
FIG. 3 is a schematic view of the structure of the cultivation process of the present invention.
Fig. 4 is a schematic perspective view of an end cap of the pressure maintaining cylinder according to the present invention.
Fig. 5 is a schematic view of the bottom structure of the inner cavity of the pressure maintaining cylinder according to the present invention.
FIG. 6 is a schematic view of the drainage process of the present invention.
Fig. 7 is a schematic structural diagram of the flap sealing mechanism and the gear mechanism of the present invention.
FIG. 8 is a flow chart of the operation method of the deep sea macro fidelity culture system of the present invention.
Detailed Description
The utility model is further described below with reference to the accompanying drawings and examples.
As shown in fig. 1-8, a deep sea macrobiosis fidelity culture device, comprises a supporting and adjusting module 7, a control module, a culture module 6 and a refrigeration module 4, wherein the supporting and adjusting module 7 is fixedly installed on a container, the culture module 6 is rotatably installed on the supporting and adjusting module 7, the refrigeration module 4 is arranged on the culture module 6, and the control module is respectively connected with the culture module 6 and the refrigeration module 4.
The culture module 6 comprises a pressure maintaining cylinder 609 and an end cover 606, the pressure maintaining cylinder 609 is a semi-closed cylinder with an opening at one end, the opening end of the pressure maintaining cylinder 609 is sealed by the end cover 606, a watertight connector II 601 is arranged on the outer end face of the end cover 606, the watertight connector II 601 is connected with the control module, a watertight connector I611 connected with the watertight connector II 601 is arranged on the inner end face of the end cover 606, the watertight connector I611 is connected with a high-pressure camera, a high-pressure lamp, a pressure sensor and a temperature sensor which are arranged in the inner cavity of the pressure maintaining cylinder 609, a butt joint hole 613 for butt joint with a sampling module and a through hole 614 for macro organisms to pass through are arranged in the center of the end cover 606, a turning plate sealing mechanism 607 for closing or opening the through hole 614 is arranged in the inner cavity of the pressure maintaining cylinder 609, and an on-off valve 605 for driving the turning plate sealing mechanism 607 is arranged on the end cover 606; the end cover 606 is provided with a plurality of high-pressure pipe connecting holes, and the inner cavity of the pressure maintaining cylinder 609 is connected with a pressure gauge 612, an overflow valve 604, a high-pressure valve 603 and an exhaust valve 608 which are arranged on the outer end surface of the end cover 606 through the high-pressure pipe connecting holes. And the end cover 606 is provided with an automatic bait delivery device 602, and the automatic bait delivery device 602 is communicated with the inner cavity of the pressure maintaining cylinder 609 through a connecting hole of a high-pressure pipe.
The flap sealing valve mechanism 607 comprises a flap valve seat 103, a flap valve cover 101, a sealing ring I102, a torsion spring 104 and a flap shaft 105, the flap valve seat 103 is connected with the pressure maintaining cylinder 609 in a sealing manner through the sealing ring 102, the flap valve seat 103 is provided with a valve hole, the valve hole is provided with the flap shaft 105, the torsion spring 104 is installed on the flap shaft 105, the flap valve cover 101 is hinged with the flap valve seat 103 through the flap shaft 105, the flap valve seat 103 is in a gear shape at the hinged position, and the flap valve seat 103 is opened and closed through a gear mechanism 9.
The gear mechanism 9 comprises an inflation connector 901, a gear rod 903, a gear rod accommodating cavity and an O-shaped sealing ring II 902, the gear rod accommodating cavity is located in the outer wall of a pressure maintaining cylinder 609, the inflation connector 901 is arranged on the pressure maintaining cylinder 609 and communicated with the gear rod accommodating cavity, the gear rod 903 is arranged in the gear rod accommodating cavity, the outer diameter of the gear rod 903 is matched with the inner diameter of the gear rod accommodating cavity, the gear rod 903 can move in the gear rod accommodating cavity, one end, far away from the inflation connector 901, of the gear rod 903 is provided with teeth, the teeth are meshed with the hinged part of a flap valve seat I603, the inflation connector 901 in the gear mechanism 9 is connected through a pressure pump to pressurize the pressure maintaining cylinder 609, the gear rod 903 moves left under the action of pressure, so that the valve cover 101 of the flap sealing valve mechanism is driven to rotate clockwise, and the outlet of the pressure maintaining cylinder 609 is opened; and the gear rod 903 and the inner wall of the gear rod accommodating cavity are sealed through an O-shaped sealing ring II 902.
The bottom of the pressure maintaining cylinder 609 is provided with a drainage device 5, the drainage device 5 comprises a high-pressure ball valve 502, a drainage cylinder 501 and a drainage valve 503, one end of the high-pressure ball valve 502 is communicated with the inner cavity of the pressure maintaining cylinder 609, the other end of the high-pressure ball valve is communicated with one end of the drainage cylinder 501, and the other end of the drainage cylinder 501 is connected with the drainage valve 503; the bottom of the inner cavity of the pressure maintaining cylinder 609 is provided with a filter plate 610 for limiting the size of the deep sea benthos, and the filter plate 610 is provided with filter holes 616.
The refrigerating module 4 comprises a condensing tube 402 and a refrigerating machine 401, the condensing tube 402 is arranged in the inner cavity of the pressure maintaining cylinder 609, connecting holes for the inlet and the outlet of the condensing tube 402 are formed in the side surface of the pressure maintaining cylinder 609, one end of the refrigerating machine 401 is connected with the inlet of the condensing tube 402, the other end of the refrigerating machine 401 is connected with the outlet of the condensing tube 402, and the refrigerating machine 401 is connected with the control module.
The control module comprises a computer 1 and a controller 2; the computer 1 is connected with the controller 2 through a cable 3, and the controller 2 is connected with the watertight connector II 601 through the cable 3; the display of the computer 1 comprises a temperature curve, a pressure curve, a real-time temperature and pressure data display window, and the control buttons of the computer 1 comprise a temperature threshold setting button, a pressure threshold setting button and a data real-time storage button.
The supporting and adjusting module 7 comprises a base 701, supporting frames 702, a motor 704 and a driven gear 705, the base 701 is installed on a container, two supporting frames 702 are symmetrically arranged on two sides of the base 701, two shaft shoulders 8 are symmetrically arranged on the side surfaces of the pressure maintaining cylinder 609, a rotating shaft 801 is installed on each shaft shoulder 8, one end of each rotating shaft 801 is installed on the corresponding shaft shoulder 8, the other end of each rotating shaft 801 is installed in a bearing seat 703 at the top of the corresponding supporting frame 702 through a bearing, a driving gear 802 is fixedly arranged on one rotating shaft 801, the driving gear 802 is meshed with the driven gear 705 below the driving gear 802, the driven gear 705 is connected with the motor 704 fixedly installed on the supporting frames 702 through a transmission shaft, and the motor 704 is connected with the controller 2. The rotating speed of the motor 704 is 1450r/min, the number of teeth of the driving gear 802 is 24, and the number of teeth of the driven gear 705 is 72.
The pressure maintaining cylinder 609 and the drainage cylinder 501 are made of 316 stainless steel, and the upper limit of pressure borne by the pressure maintaining cylinder 609, the drainage cylinder 501, the high-pressure camera, the high-pressure lamp, the pressure sensor, the temperature sensor, the overflow valve 604 and the high-pressure valve 603 is greater than 110 MPa.
A deep sea macrobiology fidelity culture method comprises the following steps:
(1) and (3) pressurizing process: before the sampling module is in butt joint with the culture module 6, seawater is injected into the culture module 6, a high-pressure valve 603 on an end cover 606 is connected through a booster pump, high-pressure water is injected into the culture module 6, a pressure sensor measures the real-time pressure in the culture module 6, a pressure signal is output to the computer 1, a display of the computer 1 displays the real-time pressure value, and the high-pressure valve 603 is closed when the pressure meets the requirement;
(2) and (3) a refrigeration process: the low-temperature water in the refrigerator 401 flows into the condenser pipe 402 through the inlet of the condenser pipe 402 so as to cool the high-pressure seawater in the pressure maintaining cylinder 609, the water in the condenser pipe 402 flows into the refrigerator 401 through the outlet of the condenser pipe 402 so as to realize recycling, the temperature sensor measures the real-time temperature in the culture module 6 and outputs a temperature real-time signal to the computer 1, and the display of the computer 1 displays a real-time temperature value so as to maintain the temperature in the macrobiotic culture module 6 at 2-4 ℃;
(3) the transfer process comprises the following steps: the sampling module is butted with the culture module 6 through a butt joint hole 613 on the end cover 606, a through hole 614 on the end cover 606 is opened through a flap sealing mechanism 607, macroorganisms in the sampling module are transferred into the inner cavity of the pressure maintaining cylinder 609 through the through hole 614, and after the macroorganisms are completely transferred, the through hole 614 on the end cover 606 is sealed through the flap sealing mechanism 607;
(4) the culture process comprises the following steps: after the macroorganisms are completely transferred to the culture module 6, the temperature and the pressure in the pressure maintaining cylinder 609 are monitored in real time through a display of the computer 1, when the pressure is lower than a set threshold value, the input end of the controller 2 receives a real-time pressure signal from a pressure sensor, and the output end of the controller 2 outputs a control signal to control a pressure pump to pressurize; when the temperature is higher than a set threshold value, the input end of the controller 2 receives a real-time temperature signal from the temperature sensor, the output end of the controller 2 outputs a control signal, and the refrigeration power of the refrigerator 401 is controlled, so that the temperature in the macro-organism culture module 6 is maintained at 2-4 ℃; automatically delivering the baits into the pressure maintaining cylinder 609 through the automatic bait delivering device 602;
(5) and (3) a drainage process: after macroorganisms are cultured in the culture module 6 for a period of time, water in the pressure maintaining cylinder 609 is replaced, the motor 704 is controlled by the controller 2 to drive the driven gear 705 to rotate, the driven gear 705 is meshed with the driving gear 802 to drive the driving gear 802 to rotate, and the rotating gear drives the rotating shaft 801 to rotate, so that the culture module 6 is driven to rotate by 90 degrees, the culture module 6 is horizontally placed, the high-pressure ball valve 502 at the bottom of the pressure maintaining cylinder 609 is opened, water in the pressure maintaining cylinder 609 flows out of the drainage cylinder 501, seawater in the pressure maintaining cylinder 609 is transferred in batches, and non-pressure drop transfer is realized; after the transfer is completed, the high pressure ball valve 502 is closed, the controller 2 controls the motor 704 to drive the driven gear 705 to rotate, so as to drive the culture module 6 to rotate by 90 degrees, and high pressure water is injected into the culture through the pressure pump, and the steps (1), (2) and (4) are continued.
Claims (9)
1. The utility model provides a deep sea macro-organism fidelity culture apparatus which characterized in that: including supporting adjustment module, control module, cultivateing module, refrigeration module, support adjustment module fixed mounting on the container, cultivate the rotatable installation of module on supporting adjustment module, be equipped with the refrigeration module on cultivateing the module, control module is connected with cultivateing module, refrigeration module respectively.
2. The deep-sea macrobiotic fidelity culture apparatus of claim 1, characterized in that: the culture module comprises a pressure maintaining cylinder and an end cover, the pressure maintaining cylinder is a semi-closed cylinder body with an opening at one end, the opening end of the pressure maintaining cylinder is sealed by the end cover, the outer end face of the end cover is provided with a watertight joint II, the watertight joint II is connected with a control module, the inner end face of the end cover is provided with a watertight joint I connected with the watertight joint II, the watertight joint I is connected with a high-pressure camera, a high-pressure lamp, a pressure sensor and a temperature sensor which are arranged in the inner cavity of the pressure maintaining cylinder, the center of the end cover is provided with a butt joint hole for butt joint with the sampling module and a through hole for macro organisms to pass through, a turning plate sealing mechanism for sealing or opening the through hole is arranged in the inner cavity of the pressure maintaining cylinder, a horn mouth is arranged on the turning plate sealing mechanism, and a switch valve for driving the turning plate sealing mechanism is arranged on the end cover; the end cover is provided with a plurality of high-pressure pipe connecting holes, and the inner cavity of the pressure maintaining cylinder is connected with a pressure gauge, an overflow valve and a high-pressure valve which are arranged on the outer end surface of the end cover through the high-pressure pipe connecting holes.
3. The deep-sea macrobiosis fidelity culture apparatus of claim 2, characterized in that: and the end cover is provided with an automatic bait delivery device which is communicated with the inner cavity of the pressure maintaining cylinder through a high-pressure pipe connecting hole.
4. The deep-sea macrobiosis fidelity culture apparatus of claim 2, characterized in that: the drainage device is arranged at the bottom of the pressure maintaining cylinder and comprises a high-pressure ball valve, a drainage cylinder and a drainage valve, one end of the high-pressure ball valve is communicated with the inner cavity of the pressure maintaining cylinder, the other end of the high-pressure ball valve is communicated with one end of the drainage cylinder, and the other end of the drainage cylinder is connected with the drainage valve; the bottom of the inner cavity of the pressure maintaining cylinder is provided with a filter plate for limiting the size of the deep sea benthos, and the filter plate is provided with filter holes.
5. The deep-sea macrobiosis fidelity culture apparatus of claim 2, characterized in that: the refrigeration module comprises a condensation pipe and a refrigerator, the condensation pipe is arranged in an inner cavity of the pressure maintaining cylinder, the side surface of the pressure maintaining cylinder is provided with a connecting hole for the outlet and the inlet of the condensation pipe, one end of the refrigerator is connected with the inlet of the condensation pipe, the other end of the refrigerator is connected with the outlet of the condensation pipe, and the refrigerator is connected with the control module.
6. The deep-sea macrobiosis fidelity culture apparatus of claim 2, characterized in that: the control module comprises a computer and a controller; the computer is connected with the controller, and the controller is connected with the watertight connector II; the display of the computer comprises a temperature curve, a pressure curve, a real-time temperature and pressure data display window, and the control buttons of the computer comprise a temperature threshold setting button, a pressure threshold setting button and a data real-time storage button.
7. The deep-sea macrobiosis fidelity culture apparatus of claim 6, characterized in that: support adjustment module and include base, support frame, motor, driven gear, pedestal mounting is on the container, and bilateral symmetry sets up two support frames on the base, pressurize section of thick bamboo side symmetry is equipped with two shoulders, all installs the axis of rotation on every shoulder, and axis of rotation one end is installed on the shoulder that corresponds the side, and the axis of rotation other end passes through the bearing to be installed in the bearing frame that is located the support frame top that corresponds the side, and one of them is fixed in the axis of rotation and is equipped with the driving gear, driving gear and the driven gear meshing that is located its below, and driven gear passes through the transmission shaft and links to each other with the motor of fixed mounting on the support frame, and the motor is connected with the controller.
8. The deep sea macro fidelity culture apparatus of claim 4, wherein: the pressure maintaining cylinder and the water draining cylinder are made of 316 stainless steel, and the upper limit of pressure borne by the pressure maintaining cylinder, the water draining cylinder, the high-pressure camera, the high-pressure lamp, the pressure sensor, the temperature sensor, the overflow valve and the high-pressure valve is greater than 110 MPa.
9. The deep-sea macrobiosis fidelity culture apparatus of claim 7, characterized in that: the rotating speed of the motor is 1450r/min, the number of teeth of the driving gear is 24, and the number of teeth of the driven gear is 72.
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| CN202023043470.6U CN215774952U (en) | 2020-12-17 | 2020-12-17 | Deep sea macrobiology fidelity culture device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112314509A (en) * | 2020-12-17 | 2021-02-05 | 中国科学院深海科学与工程研究所 | Deep sea macrobiology fidelity culture device and culture method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112314509A (en) * | 2020-12-17 | 2021-02-05 | 中国科学院深海科学与工程研究所 | Deep sea macrobiology fidelity culture device and culture method |
| CN112314509B (en) * | 2020-12-17 | 2024-05-28 | 中国科学院深海科学与工程研究所 | Deep sea macro organism fidelity culture device and culture method |
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