CN103540522A - Deep sea thermal solution simulation and high-temperature high-pressure microorganism culture system - Google Patents
Deep sea thermal solution simulation and high-temperature high-pressure microorganism culture system Download PDFInfo
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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Abstract
The invention relates to the technical field of environmental simulation and microorganism culture, and in particular relates to a deep sea thermal solution simulation and high-temperature high-pressure microorganism culture system comprising a gas and liquid transmission pipeline, and further comprising a high-pressure substrate batching unit for uniformly mixing high-pressure gas and high-pressure seawater to obtain a high-pressure seawater culture medium dissolved with high-concentration gas, a high-temperature high-pressure culture unit for acquiring the high-pressure seawater culture medium from the high-pressure substrate batching unit to breed microorganism, a gas supply unit for providing high-pressure gas for the high-pressure substrate batching unit and the high-temperature high-pressure culture unit, a flow control unit for controlling pressure in the high-pressure substrate batching unit and the high-temperature high-pressure culture unit as well as a flow velocity in a continuous culture process, and a gas monitoring control unit for detecting concentration of combustible gas in an environment, judging leakage of the combustible gas in the high-pressure substrate batching unit and the high-temperature high-pressure culture unit, and alarming in real time or automatically cutting off gas supply of the gas supply unit depending on a judgment result.
Description
Technical field
The present invention relates to microorganism culturing technical field, refer to especially a kind of deep-sea hydrothermal simulation and High Temperature High Pressure microbial culture system.
Background technology
Deep-sea is the maximum independent ecosystem in the whole world, comprise deep sea water (deeplayerwater), the geologic frameworks such as surface deposit (toplayersediment) and cold spring (coldseep), hydrothermal solution (hydrothermal vent), polymetallic nodule district (metallicnodules).The microbes biomass in biosphere, deep, deep-sea accounts for 90% of global total amount.The microorganism detecting from deep-sea and the microorganism of other environment have notable difference, have unique pathways metabolism, signal conduction and defense mechanism, are the living fossils that the early stage vital process of research and global environment develop, and have the scientific value can not be substituted.Containing " common heritage of mankind " that the abundantest Microbial resources ，Shi United Nations pays close attention to.The ocean of seawater 70% is the hyperbaric environment being in more than 38MPa.Therefore Deep-Sea Microorganisms has adapted to hyperbaric environment, has even piezophilic of resistance to pressure.High pressure is the Essential Environment parameter that deep-sea is different from other ecosystems.In addition, in the active area of plate movement, the hypomagma of being crossed by geothermal heating erupts from mid-ocean ridge zone of fracture, forms hydrothermal vent.Constantly to carry out heat exchange thereupon cooling with seawater around in ejection process for hydrothermal solution, and at basin mouth, there is obvious thermograde at place.In hydrothermal solution, be rich in the reducing substanceses such as hydrogen, sulfide, as energy derive, can be utilized by microorganism and macrofauna.Therefore,, except High Temperature High Pressure, the flow of matter of the various ways such as gas, liquid is the typical feature of hydrothermal solution environment.Because technology is restricted, be difficult to meet high temperature, high pressure simultaneously, with the environment parameter controls such as continuous flow cultivation of gaseous substrate, so the simulation of deep-sea hydrothermal system and microorganism culturing are had to certain difficulty, the research team that causes at present in the world can system carrying out the thermophilic barophilic microorganisms in deep-sea and the ecosystem is few.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of special deep-marine-environment that enlivens geology activity that has of this type of device systems simulation deep-sea hydrothermal, has solved safe way, gas-liquid mixed, temperature and pressure control, four technical barriers of continuous operation.For to the laboratory simulation of the deep-sea hydrothermal ecosystem the cultivation to hydrothermal solution microorganism, broken through a gordian technique bottleneck of Deep-Sea Microorganisms research.
For solving the problems of the technologies described above, enforcement of the present invention provides a kind of deep-sea hydrothermal simulation and High Temperature High Pressure microbial culture system, comprises for transmitting the pipeline of gas, liquid, also comprises:
High pressure substrate charger sheet unit, for even mixed high-voltage gas and high pressure sea water, obtains the high pressure sea water substratum that is dissolved with high concentrations of gas;
High Temperature High Pressure is cultivated unit, for obtaining high pressure sea water culture medium culturing microorganism from high pressure substrate charger sheet unit;
Gas supply unit, is used to high pressure substrate charger sheet unit and High Temperature High Pressure to cultivate unit high pressure gas is provided;
Flow control unit, cultivates the pressure in unit for controlling high pressure substrate charger sheet unit and High Temperature High Pressure, and the flow velocity in culture of continuous cultivation;
Gas-monitoring control unit, for detection of the concentration of inflammable gas in environment, judges in substrate batching unit and High pressure culture unit, whether inflammable gas leaks, and according to judged result and alarm or automatically cut off gas supply unit air feed.
As preferably, described gas supply unit comprises gas cylinder, relief valve, for the blowing mouth to high pressure substrate charger sheet unit delivering gas with for remaining in the venting port of the gas emptying in pipeline, between described relief valve and gas cylinder, be provided with magnetic valve, described gas-monitoring control unit is also for controlling the switch of magnetic valve; Between described venting port and gas cylinder, be provided with valve and flame-retardant device.
As preferably, high pressure substrate charger sheet unit comprises gas buffer bottle, substratum, fills the high pressure batching kettle of high pressure gas and high pressure sea water, and described gas buffer bottle lower end is by the paramount pressure batching kettle of pipeline communication; Described gas buffer bottle upper end is connected with blowing mouth in gas supply unit; Described high pressure batching kettle radial outside is socketed with the first automatically controlled heating jacket for heating; Described high pressure batching kettle upper end is provided with and prevents too high the first rupture disk and the first safety valve that causes explosion of air pressure inside; Described high pressure batching kettle is also provided with for being uniformly mixed the explosion-proof automatically controlled agitator of high pressure gas and high pressure sea water; Described high pressure batching kettle is also provided with for showing the first tensimeter of pressure in still; Described nutrient solution, by the paramount pressure batching kettle of pipeline communication, is wherein provided with the first thief hole for being communicated with the pipeline of substratum and high pressure batching kettle.
As preferably, described High Temperature High Pressure is cultivated unit and is comprised that High Temperature High Pressure cultivates still and waste liquid cylinder; Described High Temperature High Pressure is cultivated still and is connected with waste liquid cylinder by pipeline; Described High Temperature High Pressure is cultivated still radial outside and is socketed with the second automatically controlled heating jacket for heating; Described High Temperature High Pressure cultivation still upper end is provided with and prevents too high the second rupture disk and the second safety valve that causes explosion of air pressure inside; Described High Temperature High Pressure is cultivated still and is also provided with for showing the second tensimeter of pressure in still; Described high pressure batching kettle bottom is communicated to High Temperature High Pressure cultivation still still by pipeline and valve and pushes up; The pipeline that is communicated with high pressure batching kettle and High Temperature High Pressure cultivation still is provided with the second thief hole; For being communicated with the pipeline of High pressure culture still and waste liquid cylinder, be provided with the 3rd thief hole, on the pipeline between described the 3rd thief hole and back pressure valve, be also provided with for avoiding High Temperature High Pressure to cultivate flowing liquid in still and contain the strainer that particulate matter damages back pressure valve.
As preferably, described flow control unit comprises first liquid volume pump, second liquid volume pump and back pressure valve; Described first liquid volume pump is on the pipeline between substratum and the first thief hole; Described second liquid volume pump is cultivated on the pipeline between still at the second thief hole and High Temperature High Pressure; Described back pressure valve is on the pipeline between strainer and waste liquid cylinder.
As preferably, gas-monitoring control unit comprises inflammable gas detector, Controlling System and alarm unit; Described inflammable gas detector, for detection of the concentration of inflammable gas in environment; Described Controlling System, sends control information and alarm message for detecting according to inflammable gas detector when environment combustable gas concentration surpasses preset value; Alarm unit, for sending guard signal by alarm message, notifies staff combustible gas leakage; Described Controlling System is also controlled the switch of magnetic valve for sending control information.
The beneficial effect of technique scheme of the present invention is as follows: simple in structure, and reasonable in technology.The environmental parameters such as substratum composition, partial pressure, cultivation pressure, culture temperature, flow of matter speed are controlled, meet the simulation of the high temperature and high pressure environment of this type of deep-sea hydrothermal.Training method is standing type or flowing-type.In addition; in security of system, consider; we designed on source of the gas automatic alarm and automatically cut off, at batching kettle with cultivate rupture disk and safety valve duplicate protection on still, be equipped with explosion-proof module in automatically controlled stirring, select explosion-proof lamp in automatically controlled heating, can guarantee the safe operation steady in a long-term of system.Each composition of system is relatively independent in addition, has very large upgrading space.
Accompanying drawing explanation
Fig. 1 is the functional block diagram of a kind of deep-sea hydrothermal simulation of the present invention and High Temperature High Pressure microbial culture system embodiment;
Fig. 2 is the structural representation of a kind of deep-sea hydrothermal simulation of the present invention and High Temperature High Pressure microbial culture system embodiment.
Embodiment
For making the technical problem to be solved in the present invention, technical scheme and advantage clearer, be described in detail below in conjunction with the accompanying drawings and the specific embodiments.
The present invention is directed to existing deficiency a kind of deep-sea hydrothermal simulation and High Temperature High Pressure microbial culture system be provided, as shown in Figure 1, comprise for transmitting the pipeline of gas, liquid, also comprise:
High pressure substrate charger sheet unit 2, for even mixed high-voltage gas and high pressure sea water, obtains the high pressure sea water substratum that is dissolved with high concentrations of gas;
High Temperature High Pressure is cultivated unit 3, for obtaining high pressure sea water culture medium culturing microorganism from high pressure substrate charger sheet unit 2;
Gas-monitoring control unit 5, for detection of the concentration of inflammable gas in environment, judges in substrate batching unit 2 and High pressure culture unit 3, whether inflammable gas leaks, and according to judged result and alarm or automatically cut off gas supply unit 1 air feed.
As shown in Figure 2, described gas supply unit 1 comprises gas cylinder 11, relief valve 12, for the blowing mouth 13 to high pressure substrate charger sheet unit 2 delivering gas with for remaining in the venting port 14 of the gas emptying in pipeline, between described relief valve 12 and gas cylinder 11, be provided with magnetic valve 16, described gas-monitoring control unit 5 is also for controlling the switch of magnetic valve 16; Between described venting port 14 and gas cylinder 11, be provided with valve and flame-retardant device 15.
High pressure substrate charger sheet unit 2 comprises gas buffer bottle 21, substratum 22, fills the high pressure batching kettle 23 of high pressure gas and high pressure sea water, and described gas buffer bottle 21 lower ends are by the paramount pressure batching kettle 23 of pipeline communication; Described gas buffer bottle 21 upper ends are connected with blowing mouth 13 in gas supply unit 1; Described high pressure batching kettle 23 radial outsides are socketed with the first automatically controlled heating jacket 24 for heating; Described high pressure batching kettle 23 upper ends are provided with and prevent too high the first rupture disk 25 and the first safety valve 26 that causes explosion of air pressure inside; Described high pressure batching kettle 23 is also provided with for being uniformly mixed the explosion-proof automatically controlled agitator 27 of high pressure gas and high pressure sea water; Described high pressure batching kettle 23 is also provided with for showing the first tensimeter 28 of pressure in still; Described nutrient solution 22, by the paramount pressure batching kettle 23 of pipeline communication, is wherein provided with the first thief hole 29 for being communicated with the pipeline of substratum 22 and high pressure batching kettle 23.
Described High Temperature High Pressure is cultivated unit 3 and is comprised High Temperature High Pressure cultivation still 31 and waste liquid cylinder 32; Described High Temperature High Pressure is cultivated still 31 and is connected with waste liquid cylinder 32 by pipeline; Described High Temperature High Pressure is cultivated still 31 radial outsides and is socketed with the second automatically controlled heating jacket 33 for heating; Described High Temperature High Pressure cultivation still 31 upper ends are provided with and prevent too high the second rupture disk 34 and the second safety valve 35 that causes explosion of air pressure inside; Described High Temperature High Pressure is cultivated still 31 and is also provided with for showing the second tensimeter 36 of pressure in still; Described high pressure batching kettle 23 bottoms are communicated to High Temperature High Pressure cultivation still 31 stills by pipeline and valve and push up; The pipeline that is communicated with high pressure batching kettle 23 and High Temperature High Pressure cultivation still 31 is provided with the second thief hole 37; For being communicated with the pipeline of High pressure culture still 31 and waste liquid cylinder 32, be provided with the 3rd thief hole 38, on the pipeline between described the 3rd thief hole 38 and back pressure valve 43, be also provided with for avoiding High Temperature High Pressure to cultivate the interior flowing liquid of still 31 and contain the strainer 39 that particulate matter damages back pressure valve 43.
Described flow control unit 4 comprises first liquid volume pump 41, second liquid volume pump 42 and back pressure valve 43; Described first liquid volume pump 41 is on the pipeline between substratum 22 and the first thief hole 29; Described second liquid volume pump 42 is cultivated on the pipeline between still 31 at the second thief hole 37 and High Temperature High Pressure; Described back pressure valve 43 is on the pipeline between strainer 39 and waste liquid cylinder 32, and described first liquid volume pump and second liquid volume pump can be set the upper and lower bound of pressure, automatically out of service when the system fault causes pressure range to surpass set(ting)value.
Gas-monitoring control unit 5 comprises inflammable gas detector 51, Controlling System 52 and alarm unit 53; Described inflammable gas detector 51, for detection of the concentration of inflammable gas in environment; Described Controlling System 52, sends control information and alarm message for detecting according to inflammable gas detector 51 when environment combustable gas concentration surpasses preset value; Alarm unit 53, for sending guard signal by alarm message, notifies staff combustible gas leakage; Described Controlling System 52 is also controlled the switch of magnetic valve 16 for sending control information.
The above is the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of principle of the present invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (6)
1. deep-sea hydrothermal simulation and High Temperature High Pressure microbial culture system, comprise for transmitting the pipeline of gas, liquid, it is characterized in that, also comprises:
High pressure substrate charger sheet unit (2), for even mixed high-voltage gas and high pressure sea water, obtains the high pressure sea water substratum that is dissolved with high concentrations of gas;
High Temperature High Pressure is cultivated unit (3), for obtaining high pressure sea water culture medium culturing microorganism from high pressure substrate charger sheet unit (2);
Gas supply unit (1), is used to high pressure substrate charger sheet unit (2) and High Temperature High Pressure to cultivate unit (3) high pressure gas is provided;
Flow control unit (4), cultivates the pressure in unit (3) for controlling high pressure substrate charger sheet unit (2) and High Temperature High Pressure, and the flow velocity in culture of continuous cultivation;
Gas-monitoring control unit (5), concentration for detection of inflammable gas in environment, judge whether the middle inflammable gas in substrate batching unit (2) and High pressure culture unit (3) leaks, and according to judged result and alarm or automatically cut off gas supply unit (1) air feed.
2. deep-sea hydrothermal according to claim 1 is simulated and High Temperature High Pressure microbial culture system, it is characterized in that, described gas supply unit (1) comprises gas cylinder (11), relief valve (12), for the blowing mouth (13) to high pressure substrate charger sheet unit (2) delivering gas with for remaining in the venting port (14) of the gas emptying in pipeline, between described relief valve (12) and gas cylinder (11), be provided with magnetic valve (16), described gas-monitoring control unit (5) is also for controlling the switch of magnetic valve (16); Between described venting port (14) and gas cylinder (11), be provided with valve and flame-retardant device (15).
3. deep-sea hydrothermal according to claim 2 is simulated and High Temperature High Pressure microbial culture system, it is characterized in that, described high pressure substrate charger sheet unit (2) comprises gas buffer bottle (21), substratum (22), fills the high pressure batching kettle (23) of high pressure gas and high pressure sea water, and described gas buffer bottle (21) lower end is by the paramount pressure batching kettle of pipeline communication (23); Described gas buffer bottle (21) upper end is connected with blowing mouth (13) in gas supply unit (1); Described high pressure batching kettle (23) radial outside is socketed with the first automatically controlled heating jacket (24) for heating; Described high pressure batching kettle (23) upper end is provided with and prevents too high the first rupture disk (25) and the first safety valve (26) that causes explosion of air pressure inside; Described high pressure batching kettle (23) is also provided with for being uniformly mixed the explosion-proof automatically controlled agitator (27) of high pressure gas and high pressure sea water; Described high pressure batching kettle (23) is also provided with for showing first tensimeter (28) of pressure in still; Described nutrient solution (22), by the paramount pressure batching kettle of pipeline communication (23), is wherein provided with the first thief hole (29) for being communicated with the pipeline of substratum (22) and high pressure batching kettle (23).
4. deep-sea hydrothermal simulation according to claim 2 and High Temperature High Pressure microbial culture system, is characterized in that, described High Temperature High Pressure is cultivated unit (3) and comprised High Temperature High Pressure cultivation still (31) and waste liquid cylinder (32); Described High Temperature High Pressure is cultivated still (31) and is connected with waste liquid cylinder (32) by pipeline; Described High Temperature High Pressure is cultivated still (31) radial outside and is socketed with the second automatically controlled heating jacket (33) for heating; Described High Temperature High Pressure cultivation still (31) upper end is provided with and prevents too high the second rupture disk (34) and the second safety valve (35) that causes explosion of air pressure inside; Described High Temperature High Pressure is cultivated still (31) and is also provided with for showing second tensimeter (36) of pressure in still; Described high pressure batching kettle (23) bottom is communicated to High Temperature High Pressure by pipeline and valve and cultivates still (31) still top; The pipeline that is communicated with high pressure batching kettle (23) and High Temperature High Pressure cultivation still (31) is provided with the second thief hole (37); The pipeline that is used for being communicated with High pressure culture still (31) and waste liquid cylinder (32) is provided with the 3rd thief hole (38), is also provided with for avoiding High Temperature High Pressure to cultivate the interior flowing liquid of still (31) and contains the strainer (39) that particulate matter damages back pressure valve (43) on the pipeline between described the 3rd thief hole (38) and back pressure valve (43).
5. according to deep-sea hydrothermal simulation and High Temperature High Pressure microbial culture system described in claim 3 or 4, it is characterized in that, described flow control unit (4) comprises first liquid volume pump (41), second liquid volume pump (42) and back pressure valve (43); Described first liquid volume pump (41) is positioned on the pipeline between substratum (22) and the first thief hole (29); Described second liquid volume pump (42) is positioned at the second thief hole (37) and High Temperature High Pressure is cultivated on the pipeline between still (31); Described back pressure valve (43) is positioned on the pipeline between strainer (39) and waste liquid cylinder (32).
6. according to deep-sea hydrothermal simulation and High Temperature High Pressure microbial culture system described in claim 2 or 3 or 4, it is characterized in that, described gas-monitoring control unit (5) comprises inflammable gas detector (51), Controlling System (52) and alarm unit (53); Described inflammable gas detector (51), for detection of the concentration of inflammable gas in environment; Described Controlling System (52), sends control information and alarm message for detecting according to inflammable gas detector (51) when environment combustable gas concentration surpasses preset value; Alarm unit (53), for sending guard signal by alarm message, notifies staff combustible gas leakage; Described Controlling System (52) is also controlled the switch of magnetic valve (16) for sending control information.
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Cited By (10)
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| CN104195041A (en) * | 2014-07-10 | 2014-12-10 | 上海交通大学 | Standing-type electrically-controlled high temperature high pressure microbial cultivation device |
| CN105754852A (en) * | 2016-04-21 | 2016-07-13 | 东北石油大学 | Equipment and measuring method for simulating growth curves of microorganisms in high-temperature and high-pressure oil reservoirs |
| CN106644899A (en) * | 2017-02-15 | 2017-05-10 | 杭州师范大学 | Real-time haze particulate matter cytotoxicity detection device and detection method |
| CN109777731A (en) * | 2019-02-22 | 2019-05-21 | 中国船舶科学研究中心（中国船舶重工集团公司第七0二研究所） | Deep-sea hydrothermal port extreme environment simulation system |
| CN109845634A (en) * | 2019-02-21 | 2019-06-07 | 河北大学 | Super-pressure organism culturing device and its application method |
| CN112178444A (en) * | 2020-09-25 | 2021-01-05 | 江苏兴毅科技有限公司 | Automatic gas mixing and blending system |
| CN112314509A (en) * | 2020-12-17 | 2021-02-05 | 中国科学院深海科学与工程研究所 | Deep sea macrobiology fidelity culture device and culture method |
| CN114317259A (en) * | 2022-03-15 | 2022-04-12 | 南方海洋科学与工程广东省实验室(广州) | Deep sea cold spring zone membrane bioreactor and online environmental parameter measuring system |
| CN114350508A (en) * | 2022-03-17 | 2022-04-15 | 南方海洋科学与工程广东省实验室(广州) | High-pressure environment marine microorganism enrichment culture and gravity type separation device |
| CN114456909A (en) * | 2022-03-17 | 2022-05-10 | 广东工业大学 | Deep-sea microorganism separation culture device and culture method |
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