CN106644909B - Device for simulating biological sulfate formation and experimental method - Google Patents
Device for simulating biological sulfate formation and experimental method Download PDFInfo
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- CN106644909B CN106644909B CN201611082421.9A CN201611082421A CN106644909B CN 106644909 B CN106644909 B CN 106644909B CN 201611082421 A CN201611082421 A CN 201611082421A CN 106644909 B CN106644909 B CN 106644909B
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Abstract
The invention relates to a device for forming simulated biological sulfate and an experimental method, which are characterized in that: the device comprises a biological sulfate reaction device, an experimental gas generation device, a gas detection and collection device and a medium solution pumping device; the experimental gas generating device for providing the experimental gas into the biological sulfate reaction device is communicated with the biological sulfate reaction device through a corrosion-resistant gas conduit; the gas detection and collection device is used for detecting and collecting gas generated in the biological sulfate reaction device and is communicated with the biological sulfate reaction device through a corrosion-resistant gas conduit; the medium solution pumping device for pumping the medium solution of the biological sulfate reaction device is communicated with the biological sulfate reaction device through a corrosion-resistant liquid conduit. The invention can fill the technical blank of the special device for simulating the formation of biological sulfate in the prior art, and provides a special experimental device for technicians to further understand the formation process of biological sulfate.
Description
Technical Field
The invention belongs to the technical field of building experimental equipment, and particularly relates to a device for simulating biological sulfate formation and an experimental method.
Background
The corrosion resistance of concrete is one of the important indicators reflecting the durability of concrete, which is related to the service life of the concrete structure. The term concrete corrosion is generally understood to mean chemical medium corrosion. Concrete generally has a lower resistance to attack by chemical agents than other forms of corrosion. In the last century, a great deal of research work is carried out on concrete eroded by chemical media by a plurality of scholars, and the influence rule of each factor is basically examined, but the corrosive media are mainly limited to inorganic acid, alkali, salt, organic acid and other media, and the influence of microorganisms on the concrete is not involved.
At present, a great deal of research at home and abroad is focused on corrosion of microorganisms in tap water pipelines, sewage treatment plants, rivers, lakes and seawater on metal surfaces, and a great deal of construction of concrete structures is carried out to slowly attach importance to the problem of corrosion of biological sulfate formed by mineralization of the microorganisms on concrete, so that the problem needs to be solved.
In 1988, shanghai developed to treat the pollution of Suzhou river and its tributaries, improve water quality engineering, and raise the problem of preventing concrete pipe from being corroded by sewage, including microbial corrosion. The Shanghai construction sciences institute has conducted exploratory studies and the Studies of concrete cement products in Suzhou have also conducted investigations of the corrosion status of concrete drains. However, up to now, no experimental method for effectively exploring the corrosion characteristics of biological sulfate formed by mineralization of microorganisms on concrete exists in the construction field. If the sulfate itself has the properties of corroding concrete, then the biological sulfate is or is not also the same, or is more corrosive? No special equipment designed for simulating biological sulfate formation exists in the current experimental field.
Disclosure of Invention
The invention aims to solve the technical problem that no special equipment for simulating biological sulfate formation design exists in the prior art, and provides a device for simulating biological sulfate formation.
The invention adopts the technical proposal for solving the technical problems in the prior art that: an apparatus for simulating biological sulfate formation, characterized by: the device comprises a nitrogen providing device, an oxygen providing device, a first microbial reaction bin, a second microbial reaction bin, a sulfate reducing bacteria liquid providing container, a sulfur oxidizing bacteria liquid providing container, a first gas detection collecting device and a second gas detection collecting device;
the first microbial reaction bin is communicated with the second microbial reaction bin through a third gas conduit, a first stop valve is connected in series on the third gas conduit, a first gas concentration alarm is installed in the first microbial reaction bin, a second gas concentration alarm is installed in the second microbial reaction bin, and a wave making machine is installed in each of the first microbial reaction bin and the second microbial reaction bin;
the nitrogen providing device is communicated with the first microorganism reaction bin through a first gas conduit, and a first electromagnetic valve controlled by the first gas concentration alarm is connected in series on the first gas conduit;
the oxygen supply device is communicated with the second microorganism reaction bin through a second gas conduit, and a second electromagnetic valve controlled by the second gas concentration alarm is connected in series on the second gas conduit;
the sulfate reducing bacteria liquid providing container is communicated with the first microbial reaction bin through a first liquid conduit, and a first peristaltic pump is connected in series on the first liquid conduit;
the sulfur oxidizing bacteria liquid supply container is communicated with the second microorganism reaction bin through a second liquid conduit, and a second peristaltic pump is connected in series on the second liquid conduit;
the first gas detection and collection device comprises a first gas collection bottle, a first solution containing bottle, wherein the first gas collection bottle and the first solution containing bottle are communicated through a third liquid conduit, the first gas collection bottle is communicated with the first microorganism reaction bin through a fourth gas conduit, a second stop valve is connected in series on the fourth gas conduit, and a third gas concentration alarm is arranged in the first gas collection bottle;
the second gas detection and collection device comprises a second gas collection bottle, a second solution containing bottle, wherein the second gas collection bottle and the second solution containing bottle are communicated through a fourth liquid conduit, the second gas collection bottle is communicated with the second microorganism reaction bin through a fifth gas conduit, a third stop valve is connected on the fifth gas conduit in series, and a fourth gas concentration alarm is arranged in the second gas collection bottle.
The invention can also adopt the following technical measures:
the first microbial reaction bin and the second microbial reaction bin are sealed cylindrical bin bodies with sealing bolts and sealing nuts at the upper part and the lower part respectively, the first microbial reaction bin is arranged on the second microbial reaction bin, and the sealing nuts at the lower part of the first microbial reaction bin are connected with the sealing bolts at the upper part of the second microbial reaction bin.
Experimental method for simulating biological sulfate formation by using device
a) Preparing sulfate reducing bacteria liquid:
inoculating sulfate reducing bacteria into a culture medium, wherein each liter of the culture medium contains 0.4-0.6 g of dipotassium hydrogen phosphate, 0.8-1.0 g of ammonium chloride, 0.4-0.5 g of sodium sulfate, 3.2-3.5 g of sodium lactate, 18.0-20.0 g of magnesium sulfate, 0.09-0.11 g of calcium chloride, 0.8-1.0 g of yeast powder, 0.08-0.1 g of vitamin C and 0.08-0.1 g of ferrous sulfate hexahydrate; placing the culture medium in an anaerobic incubator for culturing for 2-3 days at the pH value of 6-8 and the temperature of 25-35 ℃ to obtain sulfate reducing bacteria liquid;
b) Preparing sulfur oxidizing bacteria bacterial liquid:
inoculating sulfur oxidizing bacteria into a culture medium, wherein each liter of the culture medium contains 1.0-1.2 g of disodium hydrogen phosphate, 1.8-2.0 g of monopotassium phosphate, 0.08-0.1 g of magnesium chloride, 0.08-0.1 g of ammonium chloride, 0.02-0.03 g of calcium chloride, 0.01-0.03 g of ferric chloride, 0.01-0.03 g of magnesium chloride and 9.0-11.0 g of sodium thiosulfate, and culturing the culture medium for 2-3 days under the environment of pH value of 6-8 and temperature of 25-35 ℃ under stirring at a rotating speed of 160-180 r/min to obtain sulfur oxidizing bacteria bacterial liquid;
c) Filling N in nitrogen supply device 2 Gas, O is filled in oxygen supply device 2 A gas;
d) Filling the sulfate reducing bacteria liquid prepared in the step (a) into the sulfate reducing bacteria liquid supply container, filling the sulfur oxidizing bacteria liquid prepared in the step (b) into the sulfur oxidizing bacteria liquid supply container, pumping the sulfate reducing bacteria liquid into the first microorganism reaction bin by a first peristaltic pump, and pumping the sulfur oxidizing bacteria liquid into the second microorganism reaction bin by a second peristaltic pump; opening the first electromagnetic valve, and filling the N2 gas prepared in the step (c) with the first microbial reaction bin pumped with the sulfate reducing bacteria liquid in advance; o obtained in step (c) 2 The gas is filled into a second microorganism reaction bin which is pumped with the sulfur oxidizing bacteria liquid, the normal oxygen content of the air is maintained, and the second gasThe body concentration detection alarm controls the second electromagnetic valve connected with the body concentration detection alarm to be closed, O 2 Stopping filling the gas into the second microbial reaction bin;
and opening a second stop valve and a third stop valve, and closing a first stop valve of the first microbial reaction chamber, which is communicated with the second microbial reaction chamber. When the third gas concentration alarm and the fourth gas concentration alarm in the first gas detection and collection device and the second gas detection and collection device are displayed with numbers, a second stop valve and a third stop valve which are communicated with the first gas detection and collection device and the second gas detection and collection device are closed;
opening a first stop valve of the first microbial reaction chamber, which is communicated with the second microbial reaction chamber, when H in the second microbial reaction chamber 2 When the S gas concentration reaches 10-30 ppm, the second gas concentration alarm gives an alarm and closes the first stop valve, H 2 S gas stops filling the second microbial reaction bin, and a second stop valve and a third stop valve are opened to collect redundant H 2 S gas. And generating biological sulfate in the second microbial reaction bin.
The invention has the advantages and positive effects that:
the invention can fill the technical blank of the special device for simulating the formation of biological sulfate in the prior art, and provides a special experimental device for technicians to further understand the formation process of biological sulfate.
Drawings
Fig. 1 is a schematic structural view of the present invention.
In the figure: 1. a nitrogen supply device; 2. an oxygen supply device; 3. a first microbial reaction chamber; 4. a second microbial reaction chamber; 5. a sulfate reducing bacteria liquid supply container; 6. a sulfur oxidizing bacteria liquid supply container; 7. a first gas detection collection device; 7-1, a first gas collection bottle; 7-2, a first solution containing bottle; 7-3, a third liquid conduit; 7-4, a third gas concentration alarm; 8. a second gas detection and collection device; 8-1, a second gas collection bottle; 8-2, a second solution containing bottle; 8-3, a fourth liquid conduit; 8-4, a fourth gas concentration alarm; 9. a third gas conduit; 10. a first stop valve; 11. a first gas concentration alarm; 12. a second gas concentration alarm; 13. a wave making machine; 14. a first gas conduit; 15. a first electromagnetic valve; 16. a second electromagnetic valve; 17. a first liquid conduit; 18. a first peristaltic pump; 19. a second liquid conduit; 20. a fourth gas conduit; 21. a second shut-off valve; 22. a fifth gas conduit; 23. a third stop valve; 24. a second gas conduit; 25. a second peristaltic pump.
Detailed Description
For a further understanding of the invention, its features and advantages, reference is now made to the following examples, which are illustrated in the accompanying drawings in which:
referring to fig. 1, a device for simulating biological sulfate formation includes a nitrogen gas supply device 1, an oxygen gas supply device 2, a first microbial reaction chamber 3, a second microbial reaction chamber 4, a sulfate reducing bacteria liquid supply container 5, a sulfur oxidizing bacteria liquid supply container 6, a first gas detection and collection device 7, and a second gas detection and collection device 8.
The first microbial reaction bin 2 is communicated with the second microbial reaction bin 3 through a third gas conduit 9, a first stop valve 10 is connected in series on the third gas conduit 9, a first gas concentration alarm 11 is installed in the first microbial reaction bin 3, a second gas concentration alarm 12 is installed in the second microbial reaction bin 4, and a wave making machine 13 is installed in each of the first microbial reaction bin 3 and the second microbial reaction bin 4. The first gas concentration alarm 11 and the second gas concentration alarm 12 are respectively used for detecting the gas concentrations in the first microbial reaction chamber 3 and the second microbial reaction chamber 4. The wave maker 13 is used for keeping the mobility of the medium solution in the first microbial reaction chamber 3 and the second microbial reaction chamber 4.
The nitrogen supply device 1 is communicated with the first microorganism reaction bin 3 through a first gas conduit 14, and a first electromagnetic valve 15 controlled by the first gas concentration alarm 11 is connected on the first gas conduit 14 in series. The oxygen supply device 2 is communicated with the second microorganism reaction bin 4 through a second gas conduit 24, and a second electromagnetic valve 16 controlled by the second gas concentration alarm 12 is connected on the second gas conduit 24 in series. The nitrogen supply device 1 and the oxygen supply device 2 are gas generating vessels.
The sulfate reducing bacteria liquid supply container 5 is communicated with the first microbial reaction bin 3 through a first liquid conduit 17, and a first peristaltic pump 18 is connected on the first liquid conduit 17 in series; the sulfur oxidizing bacteria liquid supply container 6 is communicated with the second microorganism reaction bin 4 through a second liquid conduit 19, and a second peristaltic pump 25 is connected on the second liquid conduit 19 in series.
The first gas detection and collection device 7 comprises a first gas collection bottle 7-1, a first solution containing bottle 7-2, wherein the first gas collection bottle 7-1 and the first solution containing bottle 7-2 are communicated through a third liquid conduit 7-3, the first gas collection bottle 7-1 and the first microorganism reaction bin 3 are communicated through a fourth gas conduit 20, a second stop valve 21 is connected on the fourth gas conduit 20 in series, and a third gas concentration alarm 7-4 is arranged in the first gas collection bottle 7-1. The second gas detection and collection device 8 comprises a second gas collection bottle 8-1, a second solution containing bottle 8-2, the second gas collection bottle 8-1 and the second solution containing bottle 8-2 are communicated through a fourth liquid conduit 8-3, the second gas collection bottle 8-1 and the second microorganism reaction bin 4 are communicated through a fifth gas conduit 22, a third stop valve 23 is connected on the fifth gas conduit 22 in series, and a fourth gas concentration alarm 8-4 is arranged in the second gas collection bottle 8-1. The first gas collecting bottle 7-2 and the second gas collecting bottle 8-2 are provided with scales.
The first microbial reaction bin 3 and the second microbial reaction bin 4 are sealed cylindrical bin bodies with sealing bolts and sealing nuts at the upper and lower parts, the first microbial reaction bin 3 is arranged on the second microbial reaction bin 4, and the sealing nuts at the lower part of the first microbial reaction bin 3 are connected with the sealing bolts at the upper part of the second microbial reaction bin 4. In this embodiment, the first microbial reaction chamber 3 and the second microbial reaction chamber 4 are communicated through two third gas conduits 9, and each gas conduit is connected in series with a first stop valve. The first gas conduit 14, the second gas conduit 24, the third gas conduit 9, the fourth gas conduit 20 and the fifth gas conduit 22 are made of corrosion resistant materials.
The experimental method for simulating biological sulfate formation by using the device is as follows:
a) Preparing sulfate reducing bacteria liquid:
inoculating sulfate reducing bacteria into a culture medium, wherein each liter of the culture medium contains 0.4-0.6 g of dipotassium hydrogen phosphate, 0.8-1.0 g of ammonium chloride, 0.4-0.5 g of sodium sulfate, 3.2-3.5 g of sodium lactate, 18.0-20.0 g of magnesium sulfate, 0.09-0.11 g of calcium chloride, 0.8-1.0 g of yeast powder, 0.08-0.1 g of vitamin C and 0.08-0.1 g of ferrous sulfate hexahydrate; placing the culture medium in an anaerobic incubator for culturing for 2-3 days at the pH value of 6-8 and the temperature of 25-35 ℃ to obtain sulfate reducing bacteria liquid.
b) Preparing sulfur oxidizing bacteria bacterial liquid:
inoculating sulfur oxidizing bacteria into a culture medium, wherein each liter of the culture medium contains 1.0-1.2 g of disodium hydrogen phosphate, 1.8-2.0 g of monopotassium phosphate, 0.08-0.1 g of magnesium chloride, 0.08-0.1 g of ammonium chloride, 0.02-0.03 g of calcium chloride, 0.01-0.03 g of ferric chloride, 0.01-0.03 g of magnesium chloride and 9.0-11.0 g of sodium thiosulfate, and culturing the culture medium for 2-3 days under the environment of pH value of 6-8 and temperature of 25-35 ℃ under stirring at a rotating speed of 160-180 r/min to obtain sulfur oxidizing bacteria bacterial liquid;
c) N is charged into a nitrogen supply device 1 2 Gas, O is filled in the oxygen supply device 2 2 And (3) gas.
d) Loading the sulfate-reducing bacteria liquid prepared in the step (a) into the sulfate-reducing bacteria liquid supply container 5, loading the sulfur-oxidizing bacteria liquid prepared in the step (b) into the sulfur-oxidizing bacteria liquid supply container 6, pumping sulfate-reducing bacteria liquid into the first microorganism reaction bin 3 by a first peristaltic pump 18, and pumping sulfur-oxidizing bacteria liquid into the second microorganism reaction bin 4 by a second peristaltic pump 25; opening the first electromagnetic valve 15 to obtain N in the step (c) 2 The first microorganism reaction bin 3 pumped with the sulfate reducing bacteria liquid is filled with gas in advance; o obtained in step (c) 2 Gas is filled into the pump to make the sulfur oxidizing bacteriaThe second microbial reaction bin 4 of the bacterial liquid keeps normal oxygen content of air, and the second gas concentration detection alarm 12 controls the closing of a second electromagnetic valve 16 connected with the second microbial reaction bin to O 2 The gas stops filling the second microbial reaction chamber 4.
The second shut-off valve 21 and the third shut-off valve 23 are opened and the first shut-off valve 10 of the first microbial reaction chamber 3 leading to the second microbial reaction chamber 4 is closed. When the third gas concentration alarm 7-4 and the fourth gas concentration alarm 8-4 in the first gas detection and collection device 7 and the second gas detection and collection device 8 are indicated, the second stop valve 21 and the third stop valve 23 which are opened to the first gas detection and collection device 7 and the second gas detection and collection device 8 are closed.
Opening the first stop valve 10 of the first microbial reaction chamber 3 to the second microbial reaction chamber 4, when H in the second microbial reaction chamber 4 2 When the S gas concentration reaches 10-30 ppm, the second gas concentration alarm 12 alarms and closes the first stop valve 10, H 2 S gas stops filling the second microorganism reaction chamber 4, and the second stop valve 21 and the third stop valve 23 are opened to collect redundant H 2 S gas. Biological sulfate is generated in the second microbial reaction chamber 4.
Claims (2)
1. An apparatus for simulating biological sulfate formation, characterized by: comprises a nitrogen providing device (1), an oxygen providing device (2), a first microorganism reaction bin (3), a second microorganism reaction bin (4), a sulfate reducing bacteria bacterial liquid providing container (5), a sulfur oxidizing bacteria bacterial liquid providing container (6), a first gas detection and collection device (7) and a second gas detection and collection device (8);
the first microbial reaction bin (3) is communicated with the second microbial reaction bin (4) through a third gas conduit (9), a first stop valve (10) is connected in series on the third gas conduit (9), a first gas concentration alarm (11) is installed in the first microbial reaction bin (3), a second gas concentration alarm (12) is installed in the second microbial reaction bin (4), and a wave making machine (13) is installed in each of the first microbial reaction bin (3) and the second microbial reaction bin (4);
the nitrogen supply device (1) is communicated with the first microorganism reaction bin (3) through a first gas conduit (14), and a first electromagnetic valve (15) controlled by the first gas concentration alarm (11) is connected in series on the first gas conduit (14);
the oxygen supply device (2) is communicated with the second microorganism reaction bin (4) through a second gas conduit (24), and a second electromagnetic valve (16) controlled by the second gas concentration alarm (12) is connected in series on the second gas conduit (24);
the nitrogen supply device (1) and the oxygen supply device (2) are gas generation containers;
the sulfate reducing bacteria liquid supply container (5) is communicated with the first microorganism reaction bin (3) through a first liquid conduit (17), and a first peristaltic pump (18) is connected on the first liquid conduit (17) in series;
the sulfur oxidizing bacteria liquid supply container (6) is communicated with the second microorganism reaction bin (4) through a second liquid conduit (19), and a second peristaltic pump (25) is connected in series on the second liquid conduit (19);
the first gas detection and collection device (7) comprises a first gas collection bottle (7-1), a first solution containing bottle (7-2), wherein the first gas collection bottle (7-1) and the first solution containing bottle (7-2) are communicated through a third liquid conduit (7-3), the first gas collection bottle (7-1) is communicated with the first microorganism reaction bin (3) through a fourth gas conduit (20), a second stop valve (21) is connected in series on the fourth gas conduit (20), and a third gas concentration alarm (7-4) is arranged in the first gas collection bottle (7-1);
the second gas detection and collection device (8) comprises a second gas collection bottle (8-1), a second solution containing bottle (8-2), wherein the second gas collection bottle (8-1) and the second solution containing bottle (8-2) are communicated through a fourth liquid conduit (8-3), the second gas collection bottle (8-1) is communicated with the second microorganism reaction bin (4) through a fifth gas conduit (22), a third stop valve (23) is connected on the fifth gas conduit (22) in series, and a fourth gas concentration alarm (8-4) is arranged in the second gas collection bottle (8-1);
the first gas collecting bottle (7-2) and the second gas collecting bottle (8-2) are provided with scales;
the first microbial reaction bin (3) and the second microbial reaction bin (4) are sealed cylindrical bin bodies with sealing bolts and sealing nuts at the upper and lower parts respectively, the first microbial reaction bin (3) is arranged on the second microbial reaction bin (4), and the sealing nuts at the lower part of the first microbial reaction bin (3) are connected with the sealing bolts at the upper part of the second microbial reaction bin (4);
the first microbial reaction bin (3) and the second microbial reaction bin (4) are communicated through two third gas conduits (9), and each gas conduit is connected with a first stop valve (10) in series.
2. An experimental method for simulating biological sulfate formation using the apparatus of claim 1, wherein: the method comprises the following steps:
a) Preparing sulfate reducing bacteria liquid:
inoculating sulfate reducing bacteria into a culture medium, wherein each liter of the culture medium contains 0.4-0.6 g of dipotassium hydrogen phosphate, 0.8-1.0 g of ammonium chloride, 0.4-0.5 g of sodium sulfate, 3.2-3.5 g of sodium lactate, 18.0-20.0 g of magnesium sulfate, 0.09-0.11 g of calcium chloride, 0.8-1.0 g of yeast powder, 0.08-0.1 g of vitamin C and 0.08-0.1 g of ferrous sulfate hexahydrate; placing the culture medium in an anaerobic incubator for culturing for 2-3 days at the pH value of 6-8 and the temperature of 25-35 ℃ to obtain sulfate reducing bacteria liquid;
b) Preparing sulfur oxidizing bacteria bacterial liquid:
inoculating sulfur oxidizing bacteria into a culture medium, wherein each liter of the culture medium contains 1.0-1.2 g of disodium hydrogen phosphate, 1.8-2.0 g of monopotassium phosphate, 0.08-0.1 g of magnesium chloride, 0.08-0.1 g of ammonium chloride, 0.02-0.03 g of calcium chloride, 0.01-0.03 g of ferric chloride, 0.01-0.03 g of magnesium chloride and 9.0-11.0 g of sodium thiosulfate, and culturing the culture medium for 2-3 days under the environment of pH value of 6-8 and temperature of 25-35 ℃ under stirring at a rotating speed of 160-180 r/min to obtain sulfur oxidizing bacteria bacterial liquid;
c) N is filled in a nitrogen supply device (1) 2 Gas, O is filled in the oxygen supply device (2) 2 A gas;
d) Filling the sulfate reducing bacteria liquid prepared in the step (a) into a sulfate reducing bacteria liquid supply container (5), filling the sulfur oxidizing bacteria liquid prepared in the step (b) into a sulfur oxidizing bacteria liquid supply container (6), pumping sulfate reducing bacteria liquid into the first microorganism reaction bin (3) by a first peristaltic pump (18), and pumping sulfur oxidizing bacteria liquid into the second microorganism reaction bin (4) by a second peristaltic pump (25); opening the first electromagnetic valve (15) to obtain N in the step (c) 2 The first microorganism reaction bin (3) which is pumped with the sulfate reducing bacteria liquid is filled with gas in advance; o obtained in step (c) 2 The second microorganism reaction bin (4) with the sulfur oxidizing bacteria liquid is filled with gas, the normal oxygen content of the air is kept, the second gas concentration detection alarm (12) controls the closing of a second electromagnetic valve (16) connected with the second gas concentration detection alarm, and O 2 Stopping filling the second microbial reaction bin (4) with gas;
opening a second stop valve (21) and a third stop valve (23), closing a first stop valve (10) of the first microorganism reaction chamber (3) which is communicated with the second microorganism reaction chamber (4), and closing the second stop valve (21) and the third stop valve (23) which are communicated with the first gas detection and collection device (7), the second gas detection and collection device (8) when the third gas concentration alarm (7-4) and the fourth gas concentration alarm (8-4) in the first gas detection and collection device (7) and the second gas detection and collection device (8) show indication;
opening a first stop valve (10) of the first microbial reaction chamber (3) which is communicated with the second microbial reaction chamber (4), and when H in the second microbial reaction chamber (4) is generated 2 When the S gas concentration reaches 10-30 ppm, the second gas concentration alarm (12) alarms and closes the first stop valve (10), H 2 S gas stops filling the second microorganism reaction bin (4), and the second stop valve (21) and the third stop valve (23) are opened to collect redundant H 2 S gas, biological sulfate is generated in the second microorganism reaction bin (4).
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| CN108398373A (en) * | 2018-04-27 | 2018-08-14 | 石家庄铁道大学 | A kind of detection method of microbiologic(al) corrosion normal concrete |
| CN113607803A (en) * | 2021-08-03 | 2021-11-05 | 天津城建大学 | Damage detection test method for simulating galvanized steel wire of bridge rope |
| CN114088609A (en) * | 2021-10-12 | 2022-02-25 | 天津城建大学 | Experimental device and experimental method for simulating microbial corrosion of concrete in seawater environment |
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| JP2008215852A (en) * | 2007-02-28 | 2008-09-18 | Nippon Steel Corp | Evaluation method of corrosion resistance of iron or iron containing alloy |
| CN101487816A (en) * | 2009-01-06 | 2009-07-22 | 哈尔滨商业大学 | Sulfate reducing bacteria double-electrolyzer test device |
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| CN103633356A (en) * | 2013-12-11 | 2014-03-12 | 山东大学 | Small microbial electrolysis cell and application thereof |
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