CN201433217Y - Pipeline biofilm sampling device - Google Patents
Pipeline biofilm sampling device Download PDFInfo
- Publication number
- CN201433217Y CN201433217Y CN 200920060140 CN200920060140U CN201433217Y CN 201433217 Y CN201433217 Y CN 201433217Y CN 200920060140 CN200920060140 CN 200920060140 CN 200920060140 U CN200920060140 U CN 200920060140U CN 201433217 Y CN201433217 Y CN 201433217Y
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- sampling
- pipeline
- biofilm
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- groove
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
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- Life Sciences & Earth Sciences (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model relates to a pipeline biofilm sampling device, which comprises a tube body, a sampling tank, a sampling plug and a biofilm slide. At least one sampling tank is arranged on the tube body and communicated with the inner cavity of the tube body, the outer port of the sampling tank is provided with the sampling plug for sealing, the biofilm slide is arranged in the tube body and is connected with the sampling tank, the sampling plug and the sampling tank can be fixed through thread connection, and the biofilm slide and the sampling plug are also fixed through thread connection. The utility model can be conveniently arranged in the pipeline net model and also in the actual pipeline, can easily sample and analyze the biofilm, and can realize the real-time monitoring of the growth of the biofilm in the pipeline. Moreover, the influence of different pipe materials on the biofilm can be examined through changing the material of the biofilm slide. In addition, the utility modelhas the advantages of simple structure, light weight and convenient installation.
Description
Technical field
The utility model relates to inner-walls of duct biological detection device, specifically, is a kind of pipeline biological film sampling apparatus that is used for the biomembranous coenosis of the inside pipe wall of water supply network, inside pipe wall burn into precipitation and sticking dirty species analysis.
Background technology
Tap water after water supply plant is handled is transported in user's the process through water transfer pipe network, owing to still have biodegradable nutrition (organism, nitrogen, phosphorus etc.) in the water, be not sterilized the bacterium that bacterium that agent kills fully or other approach enter water supply network in the output water and can utilize the biodegradable organic in the water to regrow, the part bacterium forms microbial film (Biofilm) attached to utilizing the nutraceutical matrix growth on the tube wall at random.The growth and breeding of bacterium is included in the aqueous solution suspension growth and in two kinds of forms of inside pipe wall apposition growth in the water supply network.Because tap water belongs to the poor environment of nourishing and growing, bacterium is preponderated more than suspension growth at the apposition growth of tube wall, promptly forms microbial film.Microbial film is microorganism, microorganism secretion thing and microorganism chip and the organic complex body that is adsorbed.The regrowth of suspension bacterium in the pipe network has been controlled in adding of sterilizing agent to a certain extent, but very limited for biomembranous control effect.Biomembranous existence can cause problems such as water quality on the water supply network tube wall, comprise rising, the corrosive pipeline of growth, colourity and the turbidity of pathogenic bacterium the dwindling of aggravation, water-carrying section, the generation of booster, energy consumption increases and conveyance power of water reduction etc.All be reported in both at home and abroad and detect pathogenic bacterium and conditioned pathogen in the feedwater piping microbial film, so the biomembranous growth of pipe network constitutes a threat to safety of drinking water.Analyzing the biomembranous main composition of tube wall is that research tube wall microbial film also is a most basic step to most critical in drinking water safety influence, pipe network biofilm development characteristic and the measure of control.
The biomembranous influence factor of tube wall is a lot, comprises quantity, water temperature, pipeline section material and the working life etc. of suspended bacteria in the concentration, water of waterpower factors such as nutraceutical matrix concentration in the water, flow velocity, sterilizing agent.But up to the present, relevant research still is in the junior stage, because biomembranous sampling research is relatively more difficult in the actual water supply network, and in a normal water supply network of operation microbial film small-sized stable ecosystem often, be difficult to the biomembranous generation of reality, development and changing conditions are carried out the complete and effective monitoring.
In order to realize the control to tube wall biofilm development process and growth conditions, two kinds of analogy methods of main at present employing are carried out: a kind of for being similar to the pipeline simulator of actual pipe network, another kind is the littler reactor simulation system of scale.The domestic experimental installation that is used for simulating actual pipe network microbial film forming process is mainly ring-type microbial film lacing film reactor (BAR), i.e. second kind of analogy method.The various countries scientist has also utilized BAR to carry out microbial film mensuration, and microorganism cells stripping means, microbial film morphological structure, biology can assimilate the research of organic carbon (AOC) and biodegradable dissolved organic carbon (BDOC) and biofilm biomass dependency.But ring-type microbial film lacing film reactor (BAR), just the lacing film that hangs with by rotor provides the microbial film apposition growth, rotor is rotation under driving, produce shearing force between the interface of lacing film and water body, simulate hydraulics in the pipe network with this, the size of shearing force is by regulating motor speed control, therefore, its simulated conditions differ greatly with waterpower, the condition of water quality of experiment in the pipe network, especially aspect the condition of water quality simulation, and be unrealized circulating truly of current.And can't simulate actual ducted hydraulic pressure and hydraulic detention time to the biomembranous influence of pipeline.
The utility model content
The purpose of this utility model provides a kind of biological membrane biological situation in the actual pipe network can really simulated, pipeline biological film sampling apparatus simple in structure, easy for installation.
The utility model is achieved in that the pipeline biological film sampling apparatus, comprise body, sampling groove, sampling plug and microbial film slide glass, at least one sampling groove is arranged on the body, sampling groove is connected with tube cavity, the external port of sampling groove is provided with the sealing of sampling plug, and the microbial film slide glass is connected with sampling groove in body.
Described sampling groove can be to be bonded on the body.
Described sampling plug and sampling groove can be threaded fixing.
Described microbial film slide glass is threaded fixing with the sampling plug.
Described on the transverse section of body 1, sampling groove can be provided with six, and the angle between the adjacent sampling groove can be 60 °.
Described body is a pvc pipe.
The terminal relief surface of described microbial film slide glass is provided with surface knurling, with for convenience detach.
The terminal relief surface of described sampling sample plug is provided with surface knurling, with for convenience detach.
It can be installed in the utility model in pipe network model and the actual pipeline easily, and biomembranous sampling and analysing is also relatively easy, by this device, just can monitor biomembranous upgrowth situation in the pipeline in real time, and can investigate different tubing to the film formed influence of biology by changing the material of microbial film slide glass.In addition, also has advantage simple in structure, in light weight, easy for installation.
Description of drawings
Fig. 1 is vertical section structure synoptic diagram of pipeline biological film sampling apparatus;
Fig. 2 is the section structure synoptic diagram of pipeline biological film sampling apparatus;
Fig. 3 is the sampling plug of pipeline biological film sampling apparatus and the structural representation of microbial film slide glass;
Fig. 4 is the A-A sectional view among Fig. 3;
Fig. 5 is the microbial film slide glass structural representation of pipeline biological film sampling apparatus;
Fig. 6 is the B-B sectional view among Fig. 5;
Fig. 7 is the sampling groove cut-away view of pipeline biological film sampling apparatus;
Fig. 8 is the C-C sectional view among Fig. 6.
Wherein, 1 is the pvc pipe road, and 2 are the sampling plug, and 3 is sampling groove, and 4 is the microbial film slide glass.
Embodiment
Below in conjunction with the drawings and specific embodiments the utility model pipeline biological film sampling apparatus is described in detail.
The pipeline biological film sampling apparatus as Fig. 1~shown in Figure 8, comprises body 1, sampling groove 3, sampling plug 2 and microbial film slide glass 4.A sampling groove 3 is set at least, the intracavity inter-connection of sampling groove 3 and body 1 on body 1.The external port of sampling groove 3 is provided with 2 sealings of sampling plug, and microbial film slide glass 4 is connected with sampling groove 3 in body 1.Sampling groove 3 bodys 1 are provided with a plurality of, as shown in Figure 2, can be provided with six sampling grooves 3 on the transverse section of body 1, and the angle between the adjacent sampling groove can be 60 °.Sampling plug 2 and sampling groove 3 can be threaded fixing, in order to realize sealing function, sampling fill in 2 with Packed raw material band is set between sampling groove 3 is threaded.Microbial film slide glass 4 can be fixing by being threaded with sampling plug 2.Sampling plug 2 also can adopt certain tapering according to the mechanical self-latching principle with sampling groove 3, so that can self-locking when being subjected to transverse force, and the sampling plug of avoiding being installed in below the pipeline be unlikely to because of gravity comes off, and connects closely, and water resistance is good.Body 1 can be adopted as pvc pipe, UPVC pipe or stainless steel tube, and sampling groove 3 can be to be bonded on the body 1 by caking agent.Sampling plug 2 and microbial film slide glass 4 terminal relief surface are provided with surface knurling, with for convenience detach.
Concrete use-pattern is: at first be installed in biological film sampling apparatus on the pipeline of same caliber with ring flange, after after a while, in this sampling groove, take off the sampling plug, from the sampling plug, take out the microbial film slide glass then, put into sterilized container and carry out microbial culture and counting.
The utility model is not limited to above-mentioned embodiment, if various changes of the present utility model or modification are not broken away from spirit and scope of the present utility model, if these are changed and modification belongs within claim of the present utility model and the equivalent technologies scope, then the utility model also must comprise these changes and modification.
Claims (8)
1, pipeline biological film sampling apparatus, comprise body, sampling groove, sampling plug and microbial film slide glass, it is characterized in that: at least one sampling groove is arranged on the body, sampling groove is connected with tube cavity, the external port of sampling groove is provided with the sealing of sampling plug, and the microbial film slide glass is connected with sampling groove in body.
2, pipeline biological film sampling apparatus as claimed in claim 1, it is characterized in that: described sampling groove is to be bonded on the body.
3, pipeline biological film sampling apparatus as claimed in claim 1 is characterized in that: described sampling plug and sampling groove be threaded fixing.
4, pipeline biological film sampling apparatus as claimed in claim 1 is characterized in that: described microbial film slide glass with the pipe intravital sampling plug by being threaded.
5, pipeline biological film sampling apparatus as claimed in claim 1 is characterized in that: described on the transverse section of body, sampling groove is provided with six, and the angle between the adjacent sampling groove is 60 °.
6, pipeline biological film sampling apparatus as claimed in claim 1 is characterized in that: described body is a pvc pipe.
7, pipeline biological film sampling apparatus as claimed in claim 1 is characterized in that: the terminal relief surface of described microbial film slide glass is provided with surface knurling.
8, pipeline biological film sampling apparatus as claimed in claim 1 is characterized in that: described sampling is filled in terminal relief surface and is provided with surface knurling.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 200920060140 CN201433217Y (en) | 2009-07-09 | 2009-07-09 | Pipeline biofilm sampling device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN 200920060140 CN201433217Y (en) | 2009-07-09 | 2009-07-09 | Pipeline biofilm sampling device |
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CN201433217Y true CN201433217Y (en) | 2010-03-31 |
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CN 200920060140 Expired - Fee Related CN201433217Y (en) | 2009-07-09 | 2009-07-09 | Pipeline biofilm sampling device |
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102517377A (en) * | 2011-11-19 | 2012-06-27 | 胜利油田胜利勘察设计研究院有限公司 | Method for detecting and analyzing biomembrane on inner wall of oil field sewage pipe |
CN102706688A (en) * | 2012-07-10 | 2012-10-03 | 哈尔滨工业大学 | Intelligent ball pipeline growth ring sampler without stopping water |
CN102721569A (en) * | 2012-07-10 | 2012-10-10 | 哈尔滨工业大学 | Device for sampling biomembrane on outer surface of pipe growth ring |
CN103773678A (en) * | 2014-01-27 | 2014-05-07 | 浙江大学 | Water supply pipeline biological membrane sampler and method thereof |
CN104990944A (en) * | 2015-07-02 | 2015-10-21 | 济南大学 | Oilfield sewage pipeline microbiological detection device |
CN106282307A (en) * | 2016-08-16 | 2017-01-04 | 刘平 | A kind of sampling method monitoring clinical treatment water |
CN107400699A (en) * | 2017-07-13 | 2017-11-28 | 上海市水利工程设计研究院有限公司 | A kind of pipeline biomembrane sampling system and the method for sampling based on driven by power |
-
2009
- 2009-07-09 CN CN 200920060140 patent/CN201433217Y/en not_active Expired - Fee Related
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102517377A (en) * | 2011-11-19 | 2012-06-27 | 胜利油田胜利勘察设计研究院有限公司 | Method for detecting and analyzing biomembrane on inner wall of oil field sewage pipe |
CN102706688A (en) * | 2012-07-10 | 2012-10-03 | 哈尔滨工业大学 | Intelligent ball pipeline growth ring sampler without stopping water |
CN102721569A (en) * | 2012-07-10 | 2012-10-10 | 哈尔滨工业大学 | Device for sampling biomembrane on outer surface of pipe growth ring |
CN102721569B (en) * | 2012-07-10 | 2014-01-08 | 哈尔滨工业大学 | Device for sampling biomembrane on outer surface of pipe growth ring |
CN102706688B (en) * | 2012-07-10 | 2014-06-11 | 哈尔滨工业大学 | Intelligent ball pipeline growth ring sampler without stopping water |
CN103773678A (en) * | 2014-01-27 | 2014-05-07 | 浙江大学 | Water supply pipeline biological membrane sampler and method thereof |
CN103773678B (en) * | 2014-01-27 | 2015-02-04 | 浙江大学 | Water supply pipeline biological membrane sampler and method thereof |
CN104990944A (en) * | 2015-07-02 | 2015-10-21 | 济南大学 | Oilfield sewage pipeline microbiological detection device |
CN106282307A (en) * | 2016-08-16 | 2017-01-04 | 刘平 | A kind of sampling method monitoring clinical treatment water |
CN106282307B (en) * | 2016-08-16 | 2023-07-04 | 刘平 | Sampling method for monitoring clinical treatment water |
CN107400699A (en) * | 2017-07-13 | 2017-11-28 | 上海市水利工程设计研究院有限公司 | A kind of pipeline biomembrane sampling system and the method for sampling based on driven by power |
CN107400699B (en) * | 2017-07-13 | 2024-03-15 | 上海市水利工程设计研究院有限公司 | Pipeline biological film sampling system and sampling method based on electric drive |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C17 | Cessation of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100331 Termination date: 20100709 |