CN102517377A - Method for detecting and analyzing biomembrane on inner wall of oil field sewage pipe - Google Patents
Method for detecting and analyzing biomembrane on inner wall of oil field sewage pipe Download PDFInfo
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- CN102517377A CN102517377A CN2011103875957A CN201110387595A CN102517377A CN 102517377 A CN102517377 A CN 102517377A CN 2011103875957 A CN2011103875957 A CN 2011103875957A CN 201110387595 A CN201110387595 A CN 201110387595A CN 102517377 A CN102517377 A CN 102517377A
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Abstract
The invention discloses a method for detecting and analyzing a biomembrane on the inner wall of an oil field sewage pipe. In the invention, an inner ball valve arranged on the inner wall of the oil field sewage pipe is used for controlling; a protecting sleeve is in threaded connection with the ball valve; a sealing ring at the inner periphery of the protecting sleeve presses on a seal and is in threaded connection with a pressing cap with the protecting sleeve; a positioning screw arranged on the pressing cap passes through the pressing cap, the protecting sleeve and a sampler, wherein the sampler consists of a sampling rod inserted into the inner wall of the oil field sewage pipe in the center of the ball valve and a test sheet carrier arranged at the lower end of the sampling rod; a test sheet which is made from the same material and is used as the sewage pipe is hung on the inner wall of the oil field sewage pipe; and the biomembrane of the sewage pipe is detected through the test sheet. The period for detecting the biomembrane is over 14 days. The period for detecting the biomembrane can be prolonged according to the requirement on the thickness of the biomembrane. The detected biomembrane is analyzed and detected. Bacterium amount detection, strain identification total biomass detection and the detection on the activity inhabitation ability of bactericides on bacteria in the biomembrane are respectively performed on the detected biomembrane, and an adaptive bactericide is selected from the bactericides, so the aims of good sterilizing effect, small bactericide using amount and sewage treatment cost conservation are fulfilled.
Description
Technical field
The present invention relates to a kind of biodeterioration in-situ monitoring technology; Specifically a kind of oilfield sewage inner-walls of duct microbial film measures and the microbial film analytical procedure; Through biomembranous measuring analyzed content, the flora distribution that obtains corrodibility mikrobe in the microbial film; Total biomass, microbial film characteristic and composition, the biological bactericide efficiently and the consumption thereof that adapt to of screening on this basis reaches that the sterilant consumption is few, favorable anti-corrosion effect, good in economic efficiency rot-resistant purpose.
Background technology
In the disposing polluted water in oil system, microbiological corrosion has caused metallic conduit, equipment and has seriously influenced, and receives increasing attention.Research shows that the microbial film on microbiological corrosion and equipment installation surface has confidential relation.In microbial film, mikrobe live in one with the diverse micro-ecological environment of free suspended state in, mikrobe is motionless substantially, is wrapped up in the organic matter of aquation.Cell density in the microbial film is higher than suspended state, sometimes even exceed 5～6 one magnitude, possibly produce physiology through Long contact time between the cell of consecutive position and interact, and causes collaborative microbial process.Mikrobe can not only change into insoluble biomass with component in the water, makes it to be deposited on the surface, forms dirt but also take other impurity in the water to surface, and inside becomes suitable anerobes survival environment.
In microbial film, common participation corrosive bacterial species is not a lot, generally can be divided into two big types of aerobic bacteria and anaerobic bacteriums.The bacterioid that wherein under the condition of free oxygen, could survive in the aerobic bacteria finger ring border; Mainly contain iron bacteria and thiobacterium etc.; The iron bacteria relevant with corrosion process mainly is ferrobacillus ferrooxidans; In neutrality contains the water, soil, rusty scale of organism and soluble ferric iron salt, can exist, the optimum growth temperature is 25 ℃～30 ℃, and the pH value is between 7.1～7.4.With the relevant thiobacterium of corrosion mainly is the bacterium of Thiobacillus, and like thiobacillus thiooxidant, thiobacillus thioparus and cement disintegration thiobacillus etc., wherein thiobacillus thiooxidant is the most common.Its optimum growth temperature is 28 ℃～30 ℃, and the pH value is between 2.5～3.3.Anaerobic bacterium refers to lacking free oxygen or almost do not having under the condition of free oxygen and could survive the inviable on the contrary bacterioid of aerobic.The typical anaerobic bacterium relevant with corrosion process mainly is sulphate reducing bacteria.Biomembranous primary harm is exactly the corrosion of having participated in and quickened pipeline and relevant device.Because being various mikrobes, microbial film is mingled in the population mixture that forms together under state of nature; And there is complicated ecological relationship; Therefore in fact the microbiological corrosion under the microbial film is not a kind of mikrobe result of effect separately; Often there is the effect of multiple microbial association, only single kind mikrobe had the course of doing microbiological corrosion in the very difficult expression of the exposure test actual procedure under bacterium and the aseptic condition respectively on the basis of pure culture.
In addition, microbial film can rise the colourity of water and turbidity, the bacterial number increase because reasons such as aging, flow velocity change cause and come off and worsen water quality.The biomembranous influence factor of tube wall is a lot, comprises waterpower factor, the concentration of sterilizing agent, quantity, water temperature, pipeline section material and the working lives etc. of water free bacteria such as nutraceutical matrix concentration in the water, flow velocity.But up to the present; Relevant research still is in the junior stage; Because biomembranous sampling research is relatively more difficult in the actual water supply network; And in a normal water supply network of operation microbial film small-sized stable ecosystem often, be difficult to the biomembranous generation of reality, development and changing conditions are carried out the complete and effective monitoring.Biomembranous research has caused great attention both domestic and external in recent years, and many laboratories and scholar launch research to fields such as biomembranous formation, harm, preventions, have obtained many achievements at present.
The bacterial count means mainly contain in the microbial film: the laboratory bacterial studies conventional means that microscope direct census, maximum possible bacterial count and agar plate count etc. are relevant with corrosion.Because artificial medium is different from the natural living environment of mikrobe, in culturing process, has had certain selectivity, the mikrobe that is not suitable for substratum with the competition of dominant bacteria in can be in a disadvantageous position gradually even be eliminated; Biomembranous in addition pre-treatment is difficulty, and it almost is impossible that zoogloea is broken into unicellular suspension liquid.Therefore, in fact the living weight that culture method draws is far smaller than the actual value in the physical environment, only has relative meaning.And advanced Protocols in Molecular Biology such as DGGE, PCR, FISH etc.; The detection of the multifarious detection of environmental microorganism, specific function bacterium or functional gene, the microbial population in the quantification environment etc. have been realized; But complex operation is costly, also is not suitable for operation under the complex environment.In-site detecting does not relate to separating and zoogleic fragmentation of microbial film and carrier; Mostly method therefor is biological chemistry and physics (as weighing and the microscope direct census) method etc.; Can avoid the defectives such as selectivity of cellular segregation and substratum in the culture method; Index commonly used both at home and abroad has MLSS, MLVSS, TOC, COD, the outer polymer (EPS) of born of the same parents, gross protein, Polysaccharides, peptide complexes, LPS etc.; These indexs can be divided three classes again, and what characterize like preceding four indexs is the total component of microbial film, comprises cell and acellular composition.What EPS represented is the acellular composition in the film, and back three represents intracellular component or metabolic substd, and the latter is more meaningful for desired bacterial counts alive of problem such as microorganism growth kinetics and engineering designs.Present 90～98% microbial film lipid is the phosphorus content sign with phosphatide, and easy-to-use colorimetric method for determining is applied in the research of many tap water biological treatments with its expression living weight, and is proved to be effective.
The mikrobe that swims at present adopts the means of chemical sterilization to control usually, and to the bacterial number detection means comparative maturity in the liquid medium.And the mikrobe in the microbial film is because the sampling difficulty; And because the extracellular polymeric in the microbial film has hindered the effect of chemical bactericide to bacterium in the film; Chemical bactericide is difficult to reach the purpose of killing bacterium in the film; And behind the aged biofilm detachment, bacterium is taken back flow media, cause mikrobe secondary pollution in the water surrounding.In order truly to reflect the upgrowth situation of microbial biofilm in the oil-field water system, the microorganism biological film monitoring has very important significance in the actual water body.
Summary of the invention
The purpose of this invention is to provide a kind of oilfield sewage inner-walls of duct microbial film and measure and the microbial film analytical procedure, overcome above-mentioned prior art and can only cultivate microbial film or cultivate single culture and analyze and can not onlinely measure the microbial film defective.
A kind of oilfield sewage inner-walls of duct microbial film measures method: through being controlled by the ball valve that is installed on the oilfield sewage duct wall; By the sheath that is threaded with ball valve; The sealing-ring in week in the sheath upper end, be pressed in that sealing is gone up and the pressure cap that is threaded with sheath; Be located at the set screw on the pressure cap; Pass pressure cap, sheath and ball valve center and penetrate the thief rod at sewer line inwall place and be installed in the trier that the test piece carrier of thief rod lower end is formed, will hang over sewer line inwall place with the test piece of material, measure the biological mould of sewer line through test piece with sewer line; Measure the biomembranous cycle more than 14 days, can measure the biomembranous cycle according to requirement prolongation to the microbial film thickness.
Can 4-6 trier evenly be installed in the ring week in the same cross section of inwall, oilfield sewage road for the ring week corrosion situation that measures the same cross section of oilfield sewage inner-walls of duct, the test piece that measures same cross section different azimuth is analyzed.
A kind of oilfield sewage inner-walls of duct biology mould analytical procedure: it is characterized in that carrying out the detection to bacterial activity inhibition ability in the film of the detection of bacterium amount, bacterial classification discriminating, total biomass detection and sterilant respectively according to the biological mould that the described oilfield sewage inner-walls of duct of the claim 1 microbial film method of measuring measures, its check and analysis method is:
(1) microbial film free bacteria amount detects: under the brush that the microbial film on test piece surface is careful; Be dissolved in the physiological salt soln of certain volume; Use SRB, TGB, IB bacterium test bottle respectively, utilize the bacterium amount that can be suspended in the microbial film under the disappearance dilution method pilot brush in the aqueous solution.This result can be used to weigh can dissociate in the conduit inwall microbial film and gets into the living weight in the conduit wastewater.
(2) bacterial classification is differentiated: adopt the planar surface coating method to draw the saline water diluent that contains bacterium in (1) and be coated with separation; And after cultivating under the fixed temperature and humidity condition; Form some dispersive list bacterium colonies on the plate culture medium surface; The typical single bacterium colony of picking is coated with cultivation once more respectively, obtains the purifying bacterium colony at last, further identifies.
(3) total biomass detects: other gets test piece on the parallel microbial film trier, adopts phosphorus content in the colorimetric method for determining test piece surface biological film, obtains the data of total biomass in the microbial film.This index is significant for desired bacterial counts alive such as microorganism growth kinetics and engineering designs.
(4) sterilant detects bacterial activity inhibition ability in the film: choose several kinds of sterilant and estimate the kill ability of sterilant to bacterium in the microbial film.With being suspended from biomembranous test piece in the sterilization oilfield sewage that is added with the different concns sterilant; Regularly detect the bacteria content of corrodibility mikrobe in the sewage; Follow the tracks of for some time, sterilant is compared killing bacteria ability in the microbial film, finally obtain the sterilant minimum inhibitory concentration.Because bacterium has the protection of the outer superpolymer of born of the same parents in the microbial film; The sterilant add-on that generally obtains according to the laboratory bacteria suspension is difficult to reach the purpose of killing corrodibility mikrobe in the tube wall microbial film; Do not destroying pipeline; Do not change under the situation of tube wall microbial film state, adopt this method can obtain the effect of closing to reality situation more to the screening of the microbial film original position evaluation on tube wall sterilant.
Compared with present technology the present invention has the following advantages:
1, be utilized in through trier and can measure the operating inwall microbial film of oilfield sewage pipeline, contrast is biological, and to analyze the data error that is measured little, good to high benefit sterilant, the sterilization effect of selecting to suit, practice thrift cost;
2, to the online microbial film analytical procedure that measures, can omnibearingly detect the bacterial classification bacterium amount in the microbial film, biological total amount and sterilant bacterial activity in the microbial film is suppressed ability, can optimize the corresponding good sterilant of sterilization effect;
3, on-line sampler is simple in structure, sampling safety.
Description of drawings
The mounting structure synoptic diagram of accompanying drawing-trier
The 1-thief rod; The 2-pressure-pad; The 3-set screw; The 4-sealing-ring; The 5-sheath; The 6-ball valve; 7-test piece carrier; 8-test piece retaining screw; The 9-test piece; The 10-sewer line; 11-ball valve switch handle.
Embodiment
For further disclosing technical scheme of the present invention, elaborate through embodiment below in conjunction with Figure of description:
Measuring oilfield sewage inner-walls of duct microbial film is through being installed in trier on the oilfield sewage pipeline 10; Hang over oilfield sewage inner-walls of duct place measuring biomembranous test piece 9 through thief rod 1; Through measuring the cycle more than 14 days, test piece take out obtain online biomembranous.
Specifically getting the biomembranous specific practice of oilfield sewage inner-walls of duct is, unloads the set screw 3 on the loose pressure cap 2 earlier, carries thief rod 1 with have gentle hands, because the effect of sealing-ring 4, sewage can not leak, and mentions test piece in the sheath 5.Close ball valve 6 with ball valve switch handle 11, unload lower jacket 5, unload the microbial film of promptly getting attached in the test piece to test piece retaining screw 8 from test piece carrier 7; Through to biomembranous analytical procedure step, carry out the detection of bacterium amount, bacterial classification discriminating respectively, total biomass detection and sterilant suppress the detection of ability to bacterial activity in the microbial film; Its check and analysis method is:
(1) microbial film free bacteria amount detects.Under the brush that the microbial film on test piece surface is careful, be dissolved in the physiological salt soln of certain volume, use SRB, TGB, IB bacterium test bottle respectively, utilize the bacterium amount that can be suspended in the microbial film under the disappearance dilution method pilot brush in the aqueous solution.This result can be used to weigh can dissociate in the conduit inwall microbial film and gets into the living weight in the conduit wastewater.
(2) adopt the planar surface coating method to draw the saline water diluent that contains bacterium in (1) and be coated with separation; And after cultivating under the fixed temperature and humidity condition; Form some dispersive list bacterium colonies on the plate culture medium surface; The typical single bacterium colony of picking is coated with cultivation once more respectively, obtains the purifying bacterium colony at last, further identifies.
(3) get test piece on the parallel microbial film trier in addition, adopt phosphorus content in the colorimetric method for determining test piece surface biological film, obtain the data of total biomass in the microbial film.This index is significant for desired bacterial counts alive such as microorganism growth kinetics and engineering designs.
(4) choose several kinds of sterilant and estimate the kill ability of sterilant bacterium in the microbial film.With being suspended from biomembranous test piece in the sterilization oilfield sewage that is added with the different concns sterilant; Regularly detect the bacteria content of corrodibility mikrobe in the sewage; Follow the tracks of for some time, sterilant is compared killing bacteria ability in the microbial film, finally obtain the sterilant minimum inhibitory concentration.Because bacterium has the protection of the outer superpolymer of born of the same parents in the microbial film; The sterilant add-on that generally obtains according to the laboratory bacteria suspension is difficult to reach the purpose of killing corrodibility mikrobe in the tube wall microbial film; Do not destroying pipeline; Do not change under the situation of tube wall microbial film state, adopt this method can obtain the effect of closing to reality situation more to the screening of the microbial film original position evaluation on tube wall sterilant.
Claims (3)
1. an oilfield sewage inner-walls of duct microbial film measures method: it is characterized in that through being controlled by the ball valve that is installed on the oilfield sewage duct wall; By the sheath that is threaded with ball valve; The sealing-ring in week in the sheath upper end, be pressed in that sealing is gone up and the pressure cap that is threaded with sheath; Be located at the set screw on the pressure cap; Pass pressure cap, sheath and ball valve center and penetrate the thief rod at sewer line inwall place and be installed in the trier that the test piece carrier of thief rod lower end is formed, will hang over sewer line inwall place with the test piece of material, measure the microbial film of sewer line through test piece with sewer line; Measure the biomembranous cycle more than 14 days, can measure the biomembranous cycle according to requirement prolongation to the microbial film thickness.
2. oilfield sewage inner-walls of duct microbial film according to claim 1 measures method: it is characterized in that can 4-6 trier evenly being installed in the ring week in the same cross section of inwall, oilfield sewage road for the ring week corrosion situation that measures the same cross section of oilfield sewage inner-walls of duct, the test piece that measures same cross section different azimuth is analyzed.
3. the biological mould analytical procedure of an oilfield sewage inner-walls of duct; It is characterized in that carrying out the bacterium amount respectively according to the microbial film that the described oilfield sewage inner-walls of duct of the claim 1 microbial film method of measuring measures detects, bacterial classification discriminating, total biomass detection and sterilant suppress the detection of ability to bacterial activity in the film; Its check and analysis method is:
(1) microbial film free bacteria amount detects: under the brush that the microbial film on test piece surface is careful; Be dissolved in the physiological salt soln of certain volume; Use SRB, TGB, IB bacterium test bottle respectively; Utilize the bacterium amount that can be suspended in the microbial film under the disappearance dilution method pilot brush in the aqueous solution, this result can be used to weigh can dissociate in the conduit inwall microbial film and gets into the living weight in the conduit wastewater;
(2) bacterial classification is differentiated: adopt the planar surface coating method to draw the saline water diluent that contains bacterium in (1) and be coated with separation; And after cultivating under the fixed temperature and humidity condition; Form some dispersive list bacterium colonies on the plate culture medium surface; The typical single bacterium colony of picking is coated with cultivation once more respectively, obtains the purifying bacterium colony at last, further identifies;
(3) total biomass detects: other gets test piece on the parallel microbial film trier; Adopt phosphorus content in the colorimetric method for determining test piece surface biological film; Obtain the data of total biomass in the microbial film, this index is significant for desired bacterial counts alive such as microorganism growth kinetics and engineering designs;
(4) sterilant detects bacterial activity inhibition ability in the film: choose several kinds of sterilant and estimate the kill ability of sterilant to bacterium in the microbial film.With being suspended from biomembranous test piece in the sterilization oilfield sewage that is added with the different concns sterilant; Regularly detect the bacteria content of corrodibility mikrobe in the sewage; Follow the tracks of for some time; Sterilant is compared killing bacteria ability in the microbial film, finally obtain the sterilant minimum inhibitory concentration, the protection of the outer superpolymer of born of the same parents is arranged owing to bacterium in the microbial film; The sterilant add-on that generally obtains according to the laboratory bacteria suspension is difficult to reach the purpose of killing corrodibility mikrobe in the tube wall microbial film; Not destroying pipeline, do not change under the situation of tube wall microbial film state, adopt this method can obtain the effect of closing to reality situation more to the screening of the microbial film original position evaluation on tube wall sterilant.
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Cited By (9)
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| CN102721569A (en) * | 2012-07-10 | 2012-10-10 | 哈尔滨工业大学 | Device for sampling biomembrane on outer surface of pipe growth ring |
| CN103940632A (en) * | 2014-04-28 | 2014-07-23 | 同济大学 | Biological film sampling device for pipe wall of small-pipe-diameter water supply pipe |
| CN104198543A (en) * | 2014-09-09 | 2014-12-10 | 中国石油化工股份有限公司 | Method for quickly evaluating stripping capability of bactericide to biofilm |
| CN105021783A (en) * | 2014-04-25 | 2015-11-04 | 中国石油化工股份有限公司 | Oil field on-site waste water scaling rate testing device |
| CN105154464A (en) * | 2015-10-28 | 2015-12-16 | 天津亿利科能源科技发展股份有限公司 | Method for utilizing beneficial film-forming bacteria for restraining corrosion caused by sulfate reducing bacteria in site |
| CN106124397A (en) * | 2016-08-29 | 2016-11-16 | 黄雨辰 | A kind of method of testing of oilfield sewage corrosion and scaling characteristic |
| CN107400699A (en) * | 2017-07-13 | 2017-11-28 | 上海市水利工程设计研究院有限公司 | A kind of pipeline biomembrane sampling system and the method for sampling based on driven by power |
| CN109439579A (en) * | 2018-11-14 | 2019-03-08 | 浙江海洋大学 | A kind of petroleum microorganism detection instrument case culture medium group |
| CN110592175A (en) * | 2019-09-26 | 2019-12-20 | 清华大学 | Method for screening pollution and blockage inhibiting bacteria of RO (reverse osmosis) system and method for controlling biological pollution and blockage |
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| CN102721569A (en) * | 2012-07-10 | 2012-10-10 | 哈尔滨工业大学 | Device for sampling biomembrane on outer surface of pipe growth ring |
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| CN105154464A (en) * | 2015-10-28 | 2015-12-16 | 天津亿利科能源科技发展股份有限公司 | Method for utilizing beneficial film-forming bacteria for restraining corrosion caused by sulfate reducing bacteria in site |
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| CN106124397B (en) * | 2016-08-29 | 2018-09-07 | 黄雨辰 | A kind of test method of oilfield sewage corrosion and scaling characteristic |
| CN107400699A (en) * | 2017-07-13 | 2017-11-28 | 上海市水利工程设计研究院有限公司 | A kind of pipeline biomembrane sampling system and the method for sampling based on driven by power |
| CN107400699B (en) * | 2017-07-13 | 2024-03-15 | 上海市水利工程设计研究院有限公司 | Pipeline biological film sampling system and sampling method based on electric drive |
| CN109439579A (en) * | 2018-11-14 | 2019-03-08 | 浙江海洋大学 | A kind of petroleum microorganism detection instrument case culture medium group |
| CN110592175A (en) * | 2019-09-26 | 2019-12-20 | 清华大学 | Method for screening pollution and blockage inhibiting bacteria of RO (reverse osmosis) system and method for controlling biological pollution and blockage |
| CN110592175B (en) * | 2019-09-26 | 2021-03-05 | 清华大学 | Method for screening pollution and blockage inhibiting bacteria of RO (reverse osmosis) system and method for controlling biological pollution and blockage |
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