CN107400699B - Pipeline biological film sampling system and sampling method based on electric drive - Google Patents
Pipeline biological film sampling system and sampling method based on electric drive Download PDFInfo
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- CN107400699B CN107400699B CN201710569710.XA CN201710569710A CN107400699B CN 107400699 B CN107400699 B CN 107400699B CN 201710569710 A CN201710569710 A CN 201710569710A CN 107400699 B CN107400699 B CN 107400699B
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- 238000005070 sampling Methods 0.000 title claims abstract description 145
- 238000000034 method Methods 0.000 title claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 240
- 230000007246 mechanism Effects 0.000 claims abstract description 102
- 238000005507 spraying Methods 0.000 claims abstract description 82
- 239000008223 sterile water Substances 0.000 claims description 18
- 230000005540 biological transmission Effects 0.000 claims description 13
- 238000005406 washing Methods 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 239000007921 spray Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 239000008234 soft water Substances 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 230000005484 gravity Effects 0.000 claims description 3
- 238000002386 leaching Methods 0.000 claims description 3
- 239000013049 sediment Substances 0.000 claims description 3
- 230000001680 brushing effect Effects 0.000 abstract description 6
- 230000007547 defect Effects 0.000 abstract description 4
- 241000894006 Bacteria Species 0.000 abstract description 3
- 238000011002 quantification Methods 0.000 abstract description 3
- 238000011109 contamination Methods 0.000 abstract 1
- 238000010586 diagram Methods 0.000 description 5
- 244000005700 microbiome Species 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 238000006073 displacement reaction Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 244000000010 microbial pathogen Species 0.000 description 2
- 238000011020 pilot scale process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
Abstract
The invention discloses a pipeline biological film sampling system and a sampling method based on electric drive, comprising a sampling device, a water storage device and a sampling driving and water spraying device, wherein a driving mechanism and a water spraying mechanism are integrated in the driving and water spraying device, the water spraying mechanism is connected with the water storage device, the driving mechanism is connected with the sampling device through a telescopic mechanism, and the sampling device is driven to rotate for sampling; the water spraying end of the water spraying mechanism is positioned at one side of the output end of the driving mechanism. The invention fully considers the characteristics of the biological film sampling of the actual pipeline, can be better applied to sampling conditions of different pipes and pipe diameters, avoids the contamination of mixed bacteria caused by manual sampling, and reduces the error generated during quantification. Meanwhile, researchers can conveniently sample biological films of municipal water supply pipelines with different pipes and different pipe diameters, the water quantity sprayed by brushing can be controlled during sampling, the brushing speed and time of each area of the pipeline can be adjusted according to the needs, the efficiency and convenience are considered, and some defects of the traditional method are avoided.
Description
Technical Field
The invention relates to the technical field of biological film sampling, in particular to an electric pipeline-based biological film sampling system and a sampling method, which have the characteristics of labor saving and high efficiency compared with the traditional manual biological film sampling method, and effectively improve the working efficiency of researchers in the related fields.
Background
In the water supply pipeline of the municipal pipe network system, since the microorganisms secrete extracellular polymers, a large number of microorganisms adhere to each other and to the inner wall of the pipeline, thereby forming a biofilm.
The biomembrane is favorable for pathogenic microorganisms to adapt to the change of hydraulic environment, provides necessary nutrition and growth places for the pathogenic microorganisms, and ensures various physiological metabolism activities required by survival. Therefore, most microorganisms in the pipe network are enriched in the biological film, and sampling the biological film becomes an important pre-stage link of pipeline microorganism research. However, the current pipeline biofilm sampling procedure has problems, so that the rigor and standardization of scientific research work are challenged.
The traditional manual operation is usually carried out on a pilot scale platform or a pipe dismantling site, and two methods are mainly adopted: firstly, the cotton swab is utilized to directly scrape and sample the inner wall of the pipeline, the method is more described in the related literature at present, but the efficiency and the quality of actual sampling are low, and the collected biological film sample is difficult to be represented; and the other is to use a hairbrush for sampling, vertically place the detached pipeline or heighten the pipeline at a certain inclination angle, extrude sterile water from one end of the pipeline to wash the inner wall of the pipeline by using a washing bottle, brush the biomembrane attached to the pipeline by using the hairbrush, and finally transfer the collected lining materials such as the washing liquid, the mortar and the like to the sterile sampling bottle through a tray.
The above conventional operation method has the following problems:
(1) There is no unified standard in the operational flow industry for biofilm sampling, which creates difficulties in the course of academic communication;
(2) The process is complicated, the efficiency is low, each section of pipeline needs at least one operator to brush the pipe by hand and collect the eluent, and the time spent under the condition of insufficient hands is long;
(3) The internal sampling of the pipeline cannot be guaranteed to be complete, the position, close to two ends, of the disassembled pipeline is thoroughly sampled, and the middle area of the pipeline is inevitably omitted;
(4) The municipal pipe network system has various pipe sizes, and the diameter selection of a pipe brush used for manual sampling is not flexible enough; (5) The pipeline unloaded by the pilot scale platform is stored in the experimental place for too long, is easily polluted by the medium bacteria in the air, and is difficult to meet the requirement of aseptic operation.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a pipeline biological film sampling system based on electric drive for simplifying pipeline biological film sampling operation and standardizing sampling flow. The pipeline biological film sampling system based on electric drive fully considers the characteristics of actual pipeline biological film sampling, can be better applied to sampling conditions of different pipes and pipe diameters, avoids mixed bacterial pollution caused by manual sampling, and reduces errors generated during quantification. Meanwhile, researchers can conveniently sample biological films of municipal water supply pipelines with different pipes and different pipe diameters, the water quantity sprayed by brushing can be controlled during sampling, the brushing speed and time of each area of the pipeline can be adjusted according to the needs, the efficiency and convenience are considered, and some defects of the traditional method are avoided.
The second object of the present invention is to provide a sampling method of the above-mentioned pipeline biological film sampling system based on electric driving.
The pipeline biological film sampling system based on electric drive comprises a sampling device, a water storage device and a sampling driving and water spraying device, wherein a driving mechanism and a water spraying mechanism of the sampling device are integrated in the driving and water spraying device, a water inlet end in the water spraying mechanism is connected with the water storage device, and an output end of the driving mechanism is connected with the sampling device through a telescopic mechanism and drives the sampling device to rotate for sampling through the telescopic mechanism; the water spraying end of the water spraying mechanism is positioned at one side of the output end of the driving mechanism.
In a preferred embodiment of the invention, the sampling device is a removable brush head.
In a preferred embodiment of the invention, the diameter of the removable brush head is 1cm, 3cm, 5cm or 10cm.
In a preferred embodiment of the present invention, the telescopic mechanism is a telescopic rotating shaft, the telescopic rotating shaft is formed by sequentially telescopic connection of multiple sections of rotating shafts, wherein from the sampling driving and water spraying device, a first end of a first section of rotating shaft is in transmission connection with an output end of the driving mechanism, a second end of the first section of rotating shaft is in telescopic and transmission connection with a first end of a second section of rotating shaft, and the like, a second end of a previous section of rotating shaft is in telescopic and transmission connection with a first end of a next section of rotating shaft, and a second end of a last section of rotating shaft is in detachable and transmission connection with the detachable brush head.
In a preferred embodiment of the invention, a telescopic positioning buckle is arranged between the rotating shafts of two adjacent sections, and the telescopic positioning buckle is used for realizing the fixed connection between the rotating shafts of two adjacent sections.
In a preferred embodiment of the present invention, the water storage device is formed by connecting a plurality of split-type wash bottles in series, each split-type wash bottle has a bottle body, the bottle body has a water containing cavity therein, an external screw thread joint and an internal screw thread joint are respectively provided at both ends of the bottle body, from the sampling driving and water spraying device, the internal screw thread joint of the first split-type wash bottle is screwed with the water inlet end in the water spraying mechanism, the internal screw thread joint of the second split-type wash bottle is screwed with the external screw thread joint of the first split-type wash bottle, and so on, the external screw thread joint of the last split-type wash bottle is screwed with the internal screw thread joint of the next split-type wash bottle, and a screw cap is screwed on the external screw thread joint of the last split-type wash bottle.
In a preferred embodiment of the invention, the centre of gravity of the water storage means is located on a coaxial extension of the centre axis of rotation of the sampling means.
In a preferred embodiment of the present invention, the sampling driving and water spraying device comprises a housing, and a driving mechanism and a water spraying mechanism of the sampling device integrated in the housing, wherein the driving mechanism of the sampling device comprises a rechargeable battery, a circuit board, a driving motor and a rotating main shaft forming an output end of the driving mechanism, which are electrically connected with the circuit board, and a switch arranged on the housing, the circuit board is electrically connected with the driving motor through the switch, a first end of the rotating main shaft is arranged on the housing and is in transmission connection with an output shaft of the driving motor, and a second end of the rotating main shaft extends out of the housing and is connected with a first end of a first section of rotating shaft in the telescopic rotating shaft;
the water spraying mechanism comprises a water hose arranged in the shell, a water supply switch driving mechanism arranged in the shell in a sliding manner, a water spraying pipe forming a water spraying end of the water spraying mechanism, and a water inlet connector forming a water inlet end in the water spraying mechanism, wherein a water supply switch is arranged on the water hose, a water supply driving end in the water supply switch driving mechanism is in driving connection with the water supply switch, and an operation end in the water supply switch driving mechanism is positioned outside the shell; the water spraying pipe is arranged on the shell through a 360-degree movable joint, the inlet end of the water spraying pipe is connected with one end of the soft water pipe, and the outlet end of the water spraying pipe is positioned outside the shell; the water inlet connector is arranged on the shell, the inlet end of the water inlet connector is positioned outside the shell and connected with the internal thread connector of the first spliced bottle washer, and the outlet end of the water inlet connector is connected with the other end of the water hose.
In a preferred embodiment of the present invention, a power indicator lamp and a charging port are provided on the circuit board so as to be exposed outside the housing.
In a preferred embodiment of the invention, the switch is a speed gear switch.
In a preferred embodiment of the present invention, the water spraying mechanism further comprises a pressurized water bottle, a pressurized valve and a pressurized valve return spring, wherein a valve body of the pressurized valve is mounted on a bottle body of the pressurized water bottle, one end of a piston in the pressurized valve extends into a bottle cavity of the pressurized water bottle, the pressurized valve return spring is arranged in the bottle cavity of the pressurized water bottle, one end of the pressurized valve return spring is in contact with the bottle body of the pressurized water bottle, the other end of the pressurized valve return spring is in contact with the piston in the pressurized valve, and the other end of the piston in the pressurized valve is connected with a pressurized driving end in the water supply switch driving mechanism; the water inlet connector is connected with the water inlet pipe, the water in the spliced washing bottle is controlled to flow into the water inlet pipe, and the water in the water inlet pipe cannot flow back into the spliced washing bottle.
In a preferred embodiment of the present invention, the water supply switch driving mechanism includes a trigger constituting an operation end in the water supply switch driving mechanism, a driving lever and a pair of support rods with sliding grooves, the pair of support rods with sliding grooves being installed in the housing, a pair of sliding pins being disposed on the driving lever, the pair of sliding pins being respectively slidably supported in the sliding grooves of the pair of support rods; the trigger is connected with the driving rod and is positioned outside the shell, and the driving rod is provided with a water supply driving end and a pressurizing driving end.
As a second aspect of the present invention, a sampling method of an electrically driven-based pipeline biofilm sampling system includes the steps of:
(1) Disinfection and sterilization step
The sampling starts to sterilize the water storage device and the sampling driving and water spraying device, and the sampling device, the tray for receiving the biomembrane leaching solution and the bracket are required to be subjected to high-temperature sterilization treatment;
(2) Sterile water filling step
Filling sterile water into the water storage device and the water spraying mechanism;
(3) Sampling step
Vertically placing the detached pipeline on the bracket, wherein the bracket stands in the center of the tray; selecting a proper sampling device according to the pipeline characteristics to be sampled, and adjusting the length of the telescopic mechanism to a proper position according to the pipeline characteristics to be sampled and the sampling position; then the sampling device stretches into the pipeline from one end of the pipeline, and meanwhile, a water spraying end in the water spraying mechanism stretches into the pipeline, a driving mechanism and a water spraying mechanism of the sampling device are started, sterile water is sprayed on the inner wall of the pipeline by the water spraying mechanism, the driving mechanism of the sampling device drives the sampling device to rotate at a certain speed so as to rub the inner wall of the pipeline, and a biological film and sediments attached to the inner wall of the pipeline flow into a tray along with sprayed sterile water; after sampling is finished, the driving mechanism and the water spraying mechanism of the sampling device are closed, the sampling device is taken out from the pipeline to take the sampling device out, then the pipeline and the bracket are moved out of the tray, and the adopted materials collected in the tray are transferred into an aseptic sampling bottle;
if the pipe is too long, the above step (3) may be repeated from the other end of the pipe.
Due to the adoption of the technical scheme, the pipeline biological film sampling system based on electric drive fully considers the characteristics of actual pipeline biological film sampling, can be better applied to sampling conditions of different pipes and pipe diameters, avoids mixed bacteria pollution caused by manual sampling, and reduces errors generated during quantification. Meanwhile, researchers can conveniently sample biological films of municipal water supply pipelines with different pipes and different pipe diameters, the water quantity sprayed by brushing can be controlled during sampling, the brushing speed and time of each area of the pipeline can be adjusted according to the needs, the efficiency and convenience are considered, and some defects of the traditional method are avoided.
Drawings
Fig. 1 is a schematic diagram of a pipeline biological film sampling system based on electric driving.
Fig. 2 is a schematic structural diagram of a sampling device in the pipeline biological film sampling system based on electric driving.
Fig. 3 is a schematic structural diagram of a telescopic mechanism in the pipeline biological membrane sampling system based on electric driving.
Fig. 4 is a schematic diagram of a sampling driving and water spraying device in the pipeline biological film sampling system based on electric driving.
Fig. 5 is a schematic diagram of the structure of a detachable bottle washer in the electric drive-based pipeline biofilm sampling system.
Detailed Description
Referring to fig. 1, an electrically-driven pipe bio-film sampling system is shown, which includes a sampling device 100, a water storage device 200 and a sampling driving and spraying device 300, wherein a driving mechanism and a spraying mechanism (described in detail below) of the sampling device are integrated in the driving and spraying device 300, a water inlet end of the spraying mechanism is connected with the water storage device 200, and an output end of the driving mechanism is connected with the sampling device 100 through a telescopic mechanism 400 and drives the sampling device 100 to rotate for sampling through the telescopic mechanism; the water spraying end of the water spraying mechanism is positioned at one side of the output end of the driving mechanism.
Referring to fig. 2, the sampling device 100 is a removable brush head 110, the removable brush head 110 is provided with four sets of different specifications, the diameters of the removable brush head 110 are 1cm, 3cm, 5cm and 10cm, and the diameter of the removable brush head can be independently selected according to the characteristics of the operation pipe section. DN40 and below, DN125 and below, DN3.5.10.110. The detachable brush head 110 is connected with a rotating shaft lever 120, and one end of the rotating shaft lever 120 connected with the telescopic mechanism 400 is provided with a hexagonal head 121, so that after the detachable brush head is connected with the telescopic mechanism 400, a displacement phenomenon can not occur during rotation.
Referring to fig. 3, the telescopic mechanism 400 is a telescopic rotating shaft, which is formed by sequentially connecting three rotating shafts 410, 420 and 430 in a telescopic manner, wherein the three rotating shafts 410, 420 and 430 are made of stainless steel materials, the diameter is from thick to thin, the rotating shaft 410 is thickest, the rotating shaft 420 is thinner, and the rotating shaft 430 is thinnest. The lengths of the three rotary shafts 410, 420, 430 are 208mm, 200mm, respectively. One end of the rotating shaft 410 is in transmission connection with the output end of the driving mechanism, the other end of the rotating shaft 410 is in telescopic and transmission connection with one end of the rotating shaft 420, the other end of the rotating shaft 420 is in telescopic and transmission connection with one end of the rotating shaft 430, a hexagonal inner hole 431 is formed in the other end of the rotating shaft 430, and the hexagonal head 121 on the rotating shaft rod 120 is inserted into the hexagonal inner hole 431 in the rotating shaft 430 when connected, so that displacement phenomenon cannot occur when the two are rotated.
The telescopic locating buckle 440 is arranged between the rotating shaft 410 and the rotating shaft 420, the telescopic locating buckle 450 is arranged between the rotating shaft 420 and the rotating shaft 430, when the telescopic locating buckle is not in operation, the rotating shaft 420 is retracted into the inner hole of the rotating shaft 410, the rotating shaft 430 is retracted into the inner hole of the rotating shaft 420, when the telescopic locating buckle 440 is operated, the rotating shaft 420 extends out of the rotating shaft 410 for a certain length, and the rotating shaft 430 extends out of the rotating shaft 420 for a certain length through the telescopic locating buckle 450, so that the total length of the telescopic mechanism 400 is changed.
Referring to fig. 1 and 5 in combination, the water storage device 200 is formed by connecting two wash bottles 210, 220 in series (or a plurality of wash bottles are also possible). The capacity of the spliced washing bottles 210 and 220 is 250mL, and the spliced washing bottles are made of PVC materials. The bottle-washing units 210 and 220 are respectively provided with a bottle body 211 and 221, and the bottle bodies 211 and 221 are internally provided with a water containing cavity. An externally threaded joint 212, 222 and an internally threaded joint 213, 223 are provided at both ends of the bottle body 211, 221, respectively.
The inner threaded joint 213 of the spliced washer 210 is in threaded connection with the water inlet end of the water spraying mechanism, the inner threaded joint 223 of the spliced washer 220 is in threaded connection with the outer threaded joint 212 of the spliced washer 210, and a screw cap 230 is screwed on the outer threaded joint 213 of the spliced washer 220. The centers of gravity of the two splice-able wash bottles 210, 220 are located on a coaxial extension of the central axis of rotation of the sampling device 100, which maintains a balanced and stable operation of the electrically driven pipeline biofilm sampling system.
Referring to fig. 3, the sampling driving and water spraying device 300 includes a housing 310 and a driving mechanism and a water spraying mechanism of the sampling device integrated in the housing 310.
The driving mechanism of the sampling device includes a rechargeable battery 321, a circuit board 322, a driving motor 323, a rotating main shaft 324 forming an output end of the driving mechanism, and a rotation speed gear switch 325 provided on the housing 310, wherein the rechargeable battery 321 is electrically connected with the circuit board 322, and the circuit board 322 is electrically connected with the driving motor 323 through the rotation speed gear switch 325.
The driving motor 323 is supported in the housing 310 through a motor bracket 326, an output shaft of the driving motor 323 is in transmission connection with one end of the rotating main shaft 324, the rotating main shaft 324 is axially arranged on the housing 310 through a bearing 327, and the other end of the rotating main shaft 324 extends out of the housing 310 and is connected with a rotating shaft 410 of the telescopic rotating shaft.
The speed switch 325 is provided with four gears of high speed, medium speed, low speed and stop to control the speed of the driving motor 232 to correspond to different sampling requirements.
The circuit 322 is provided with a power indicator 328 and a charging port 329 exposed to the outside of the case 310, and the rechargeable battery 321 can be charged through the charging port 329 and the circuit 322.
The water spraying mechanism includes a flexible water pipe 331 provided in the housing 310, a water supply switch driving mechanism slidably disposed in the housing 310, a pressurized water bottle 334 provided in the housing 310, a pressurized valve 335, and a pressurized valve return spring 336, and further includes a water spraying pipe 332 constituting a water spraying end of the water spraying mechanism and a water inlet joint 333 constituting a water inlet end in the water spraying mechanism.
A hard tube 337 is installed in the housing 310, and a soft water tube 331 is installed in the hard tube 337 to protect the soft water tube 331. A water supply switch 331a is disposed on the water hose 331, and one end of the water hose 331 may be directly connected to the water inlet connector 333 or may be connected to the pressurized water bottle 334, in which embodiment of the present invention, one end of the water hose 331 is connected to the pressurized water bottle 334.
The spout 332 is mounted to the housing 310 by a 360 ° articulation 338 such that the outlet angle of the spout 332 is freely adjustable. The 360 ° articulation joint 338 may be a ball. The other end of the flexible water pipe 331 is connected to the inlet end of the water spraying pipe 332, and the outlet end of the water spraying pipe 332 is located outside the housing 310.
The valve body of the pressure valve 335 is mounted on the body of the pressure water bottle 334, one end of the piston in the pressure valve 335 extends into the cavity of the pressure water bottle 334, the pressure valve return spring 336 is disposed in the cavity of the pressure water bottle 334, one end of the pressure valve return spring 336 contacts the body of the pressure water bottle 334, and the other end contacts the piston in the pressure valve 335.
A water inlet connector 333 is mounted to the housing 310, and the outlet end of the water inlet connector 333 is connected to a pressurized water bottle 334 through a one-way valve (not shown) that controls the flow of water from within the splice-able wash bottles 210, 220 into the pressurized water bottle 334, and the water within the pressurized water bottle 334 does not flow back into the splice-able wash bottles 210, 220. The inlet end of the water inlet connector 333 is located outside the housing 310 and is connected to the internally threaded connector 213 of the splice-able wash bottle 210.
The water supply switch driving mechanism includes a trigger 411, a driving lever 412 and a pair of support rods 413, 414 with sliding grooves 413a, 414a, the pair of support rods 413, 414 with sliding grooves 413a, 414a being installed in the housing 310, a pair of sliding pins 412a, 412b being disposed on the driving lever 412, the pair of sliding pins 412a, 412b being respectively slidably supported in the sliding grooves 413a, 414a of the pair of support rods 413, 414; trigger 411 is coupled to drive rod 412 and is located outside of housing 310,
the driving rod 412 is provided with a water supply driving end 412c and a pressurizing driving end 412d, the water supply driving end 412c is in driving connection with the water supply switch 331a, and the pressurizing driving end 412d is in driving connection with the other end of the piston in the pressurizing valve 335.
In a natural state, the trigger 411 is not pulled by a finger, the piston in the pressure valve 335 and the driving rod 412 in the water supply switch driving mechanism are both in the initial position under the action of the pressure valve return spring 336, the water supply switch 331a is in the closed state, and the sterile water in the two spliced wash bottles 210, 220 and the pressurized water bottle 334 does not flow into the water spray pipe 332 through the soft water pipe 331 and is sprayed out of the water spray pipe 332. In the working state, the trigger 411 is pulled by a finger, the trigger 411 drives the driving rod 412 to move towards the working position, the pressurizing driving end 412d on the driving rod 412 forces the piston in the pressurizing valve 335 to move towards the pressurizing direction after overcoming the resistance of the pressurizing valve return spring 336, and meanwhile, the water supply driving end 412c on the driving rod 412 opens the water supply switch 331a, and at this time, the sterile water in the pressurizing water bottle 334 flows into the water spraying pipe 332 through the water hose 331 and is sprayed out by the water spraying pipe 332. The trigger 411 is released, the piston in the pressure valve 335 and the drive rod 412 in the water supply switch drive mechanism return to the original position under the action of the pressure valve return spring 336, at which time the pressure in the pressurized water bottle 334 is reduced and the one-way valve begins and sterile water in the splice wash bottles 210, 220 can be replenished into the pressurized water bottle 334.
The sampling method of the pipeline biological film sampling system based on electric drive comprises the following steps:
(1) Disinfection and sterilization step
The sampling starts to sterilize the spliced washing bottles 210 and 220 and the sampling driving and water spraying device 300 in the water storage device 200 by alcohol, and the detachable brush head 110, the tray and the bracket for receiving the biomembrane leaching liquid in the sampling device 100 are required to be sterilized at high temperature;
(2) Sterile water filling step
Filling sterile water into the spliced wash bottles 210, 220 in the water storage device 200 and the pressurized water bottle 334 in the water spraying mechanism;
(3) Sampling step
The detachable pipeline is vertically arranged on a bracket, and the bracket stands in the center of the tray; selecting a proper detachable brush head 110 according to the pipeline characteristics to be sampled, and adjusting the length of the telescopic mechanism 400 to a proper position according to the pipeline characteristics to be sampled and the sampling position; then the detachable brush head 110 is extended into the pipeline from one end of the pipeline, meanwhile, the spray pipe 332 in the water spraying mechanism is extended into the pipeline, the trigger 411 is pulled by fingers, the trigger 411 drives the driving rod 412 to move towards the working position direction, the pressurizing driving end 412d on the driving rod 412 forces the piston in the pressurizing valve 335 to move towards the pressurizing direction after overcoming the resistance of the pressurizing valve reset spring 336, meanwhile, the water supply driving end 412c on the driving rod 412 opens the water supply switch 331a, at the moment, the sterile water in the pressurizing water bottle 334 flows into the spray pipe 332 through the water hose 331 and is sprayed out by the spray pipe 332, and the sterile water is sprayed on the inner wall of the pipeline.
Simultaneously, the rotation speed of the driving motor 323 is regulated by using the rotation speed gear switch 325, the driving motor 323 drives the detachable brush head 110 to rotate at a certain speed through the telescopic mechanism 400 so as to rub the inner wall of the pipeline, and the biomembrane and sediment attached to the inner wall of the pipeline flow into the tray along with the sprayed sterile water; after sampling, the trigger 411 is released, the piston in the pressure valve 335 and the driving rod 412 in the water supply switch driving mechanism are all in the initial position under the action of the pressure valve return spring 336, the water supply switch 331a is in the closed state, and the sterile water in the two spliced wash bottles 210, 220 and the pressurized water bottle 334 cannot flow into the water spraying pipe 332 through the water hose 331 and be sprayed out by the water spraying pipe 332. And simultaneously rotates the speed shift switch 325 to a stop. The removable brush head 110 is taken out of the pipeline, then the pipeline and the bracket are moved out of the tray, and the collected adopted objects in the tray are transferred into a sterile sampling bottle;
if the pipe is too long, the above step (3) may be repeated from the other end of the pipe.
During sampling, the rotation speed gear switch 325 can be set to a high-speed gear according to the actual condition of the inner wall of the pipeline at the beginning of sampling, and is adjusted to a low-speed gear before the end of sampling.
Claims (10)
1. The pipeline biological film sampling system based on electric drive is characterized by comprising a sampling device, a water storage device and a sampling driving and water spraying device, wherein a driving mechanism and a water spraying mechanism of the sampling device are integrated in the sampling driving and water spraying device, a water inlet end in the water spraying mechanism is connected with the water storage device, and an output end of the driving mechanism is connected with the sampling device through a telescopic mechanism and drives the sampling device to rotate for sampling through the telescopic mechanism; the water spraying end in the water spraying mechanism is positioned at one side of the output end of the driving mechanism;
the sampling device is a detachable brush head;
the diameter of the detachable brush head is 1cm, 3cm, 5cm or 10cm;
the telescopic mechanism is a telescopic rotating shaft which is formed by sequentially telescopic connection of a plurality of sections of rotating shafts, wherein from the sampling driving and water spraying device, the first end of a first section of rotating shaft is in transmission connection with the output end of the driving mechanism, the second end of the first section of rotating shaft is in telescopic transmission connection with the first end of a second section of rotating shaft, and the second end of a previous section of rotating shaft is in telescopic transmission connection with the first end of a next section of rotating shaft, and the second end of a last section of rotating shaft is in detachable transmission connection with the detachable brush head.
2. The electrically driven pipeline biofilm sampling system of claim 1, wherein a telescopic positioning buckle is arranged between two adjacent sections of rotating shafts, and the telescopic positioning buckle is used for realizing fixed connection between the two adjacent sections of rotating shafts.
3. The pipeline biological film sampling system based on electric drive as claimed in claim 1, wherein the water storage device is formed by connecting a plurality of spliced wash bottles in series, each spliced wash bottle is provided with a bottle body, a water containing cavity is arranged in the bottle body, two ends of the bottle body are respectively provided with an external thread joint and an internal thread joint, from the sampling drive and water spraying device, the internal thread joint of the first spliced wash bottle is in threaded connection with the water inlet end in the water spraying mechanism, the internal thread joint of the second spliced wash bottle is in threaded connection with the external thread joint of the first spliced wash bottle, and the external thread joint of the last spliced wash bottle is in threaded connection with the internal thread joint of the next spliced wash bottle, and a screw cap is screwed on the external thread joint of the last spliced wash bottle.
4. A pipeline biological film sampling system based on electric drive according to claim 3, characterized in that the centre of gravity of the water storage device is located on the coaxial extension of the central axis of rotation of the sampling device.
5. An electrically driven pipeline biofilm sampling system as claimed in any of claims 1 to 4 including a housing and drive and water spray means for said sampling means integrated within said housing, wherein said drive means for said sampling means includes a rechargeable battery disposed within said housing, a circuit board, a drive motor and a rotating spindle forming an output of said drive means, said rechargeable battery being electrically connected to said circuit board, said circuit board being electrically connected to said drive motor by said switch, said rotating spindle being journalled on said housing and having a first end in driving connection with an output shaft of said drive motor, and a second end of said rotating spindle extending out of said housing and being connected to a first end of a first one of said rotatable shafts;
the water spraying mechanism comprises a water hose arranged in the shell, a water supply switch driving mechanism arranged in the shell in a sliding manner, a water spraying pipe forming a water spraying end of the water spraying mechanism, and a water inlet connector forming a water inlet end in the water spraying mechanism, wherein a water supply switch is arranged on the water hose, a water supply driving end in the water supply switch driving mechanism is in driving connection with the water supply switch, and an operation end in the water supply switch driving mechanism is positioned outside the shell; the water spraying pipe is arranged on the shell through a 360-degree movable joint, the inlet end of the water spraying pipe is connected with one end of the soft water pipe, and the outlet end of the water spraying pipe is positioned outside the shell; the water inlet connector is arranged on the shell, the inlet end of the water inlet connector is positioned outside the shell and connected with the internal thread connector of the first spliced bottle washer, and the outlet end of the water inlet connector is connected with the other end of the water hose.
6. An electrically driven pipeline biofilm sampling system as in claim 5 wherein a power indicator light and a charging port are provided on said circuit board exposed out of said housing.
7. An electrically driven pipeline biofilm sampling system as claimed in claim 5 wherein said switch is a speed shift switch.
8. The electric drive-based pipeline biological film sampling system according to claim 5, wherein the water spraying mechanism further comprises a pressurized water bottle, a pressurized valve and a pressurized valve return spring, a valve body of the pressurized valve is arranged on a bottle body of the pressurized water bottle, one end of a piston in the pressurized valve stretches into a bottle cavity of the pressurized water bottle, the pressurized valve return spring is arranged in the bottle cavity of the pressurized water bottle, one end of the pressurized valve return spring is in contact with the pressurized water bottle, the other end of the pressurized valve return spring is in contact with a piston in the pressurized valve, and the other end of the piston in the pressurized valve is connected with a pressurized driving end in the water supply switch driving mechanism; the water inlet connector is connected with the water inlet pipe, the water in the spliced washing bottle is controlled to flow into the water inlet pipe, and the water in the water inlet pipe cannot flow back into the spliced washing bottle.
9. The electrically driven pipeline biofilm sampling system of claim 5 wherein said water switch drive mechanism includes a trigger constituting an operating end in said water switch drive mechanism, a drive rod and a pair of support rods with slide slots, a pair of support rods with slide slots being mounted in said housing, a pair of slide pins being disposed on said drive rod, a pair of slide pins being slidably supported in the slide slots of a pair of support rods, respectively; the trigger is connected with the driving rod and is positioned outside the shell, and the driving rod is provided with a water supply driving end and a pressurizing driving end.
10. The sampling method of the electrically driven pipeline biological film sampling system as claimed in claim 1, comprising the steps of:
(1) Disinfection and sterilization step
The sampling starts to sterilize the water storage device and the sampling driving and water spraying device, and the sampling device, the tray for receiving the biomembrane leaching solution and the bracket are required to be subjected to high-temperature sterilization treatment;
(2) Sterile water filling step
Filling sterile water into the water storage device and the water spraying mechanism;
(3) Sampling step
Vertically placing the detached pipeline on the bracket, wherein the bracket stands in the center of the tray; selecting a proper sampling device according to the pipeline characteristics to be sampled, and adjusting the length of the telescopic mechanism to a proper position according to the pipeline characteristics to be sampled and the sampling position; then the sampling device stretches into the pipeline from one end of the pipeline, and meanwhile, a water spraying end in the water spraying mechanism stretches into the pipeline, a driving mechanism and a water spraying mechanism of the sampling device are started, sterile water is sprayed on the inner wall of the pipeline by the water spraying mechanism, the driving mechanism of the sampling device drives the sampling device to rotate at a certain speed so as to rub the inner wall of the pipeline, and a biological film and sediments attached to the inner wall of the pipeline flow into a tray along with sprayed sterile water; after sampling is finished, the driving mechanism and the water spraying mechanism of the sampling device are closed, the sampling device is taken out from the pipeline to take the sampling device out, then the pipeline and the bracket are moved out of the tray, and the adopted materials collected in the tray are transferred into an aseptic sampling bottle;
if the pipe is too long, repeating the step (3) from the other end of the pipe.
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| CN112831400B (en) * | 2020-12-14 | 2023-01-31 | 北京市理化分析测试中心 | Solid surface microorganism sampling method |
| CN112725164B (en) * | 2020-12-14 | 2023-02-17 | 北京市理化分析测试中心 | Solid surface microorganism sampler |
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