CN103966080A - Enrichment sampling device for microorganisms in water of water supply pipe network - Google Patents
Enrichment sampling device for microorganisms in water of water supply pipe network Download PDFInfo
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- CN103966080A CN103966080A CN201410191554.4A CN201410191554A CN103966080A CN 103966080 A CN103966080 A CN 103966080A CN 201410191554 A CN201410191554 A CN 201410191554A CN 103966080 A CN103966080 A CN 103966080A
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- water
- pipe
- water supply
- protruded packing
- sampling unit
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
Abstract
The invention discloses an enrichment sampling device for microorganisms in water of a water supply pipe network. The device comprises a water supply pipe (4) and further comprises a rigid rope (1), a casing pipe (2), a porous filler (3), a screen (9) and a seal washer (10), wherein the casing pipe (2) and the porous filler (3) are arranged at an interval and strung together through the rigid rope (1) to form a filler string, the filler string is threaded in the water supply pipe (4), the two ends of the rigid rope (1) are fixed on the screen (9) arranged on the end surface of the water supply pipe (4), and the screen (9) is clamped by the abutment arrangement of the end part of the water supply pipe (4) and the adjacent pipe section, the seal washer (10) is padded and then connection and fixation are realized. Compared with the prior art, the enrichment sampling device has the advantages that the device is simple to assemble and convenient for sampling, and the uniform position fixing and distribution of the porous filler is favorable to the enrichment of the microorganisms.
Description
Technical field
The present invention relates to microorganism enrichment sampling unit in a kind of water supply network pipe network water, especially relate to the sampling unit that a kind of protruded packing is realized microorganism enrichment.
Background technology
The secondary pollution problem of water supply network is the study hotspot in current tap water field, and the microbial safety of tap water is one of most important link of drinking-water quality safety.Microorganism in water supply network be mainly distributed in pipe network water and tube wall microbial film in.Tube wall microbial film is microorganism, microorganism secretion thing and microorganism chip and the organic complex body that is adsorbed; there is absorption microorganism; protection microorganism is hidden the effects such as sterilizing agent impact; be more conducive to microbial growth, so tube wall microbial film is the main place of microorganism existence in water supply network.
Research in recent years shows, the factors such as water conservancy condition changes, microbial film is aging all can cause that tube wall biofilm detachment enters in main body pipe network water, in addition because water supply network pipeline is long, disinfectant residual reduces gradually, restraining effect to microorganism growth weakens, also can cause the increase of microbe population in pipe network water, thereby cause drinking-water quality to decline, serious threat drinking-water quality safety.So the research to microorganism in water supply network pipe network water has important practical significance.
In reality, in water supply network pipe network water microorganism normally by using the water sample of small-bore membrane filtration certain volume to realize microorganism enrichment, but this kind of method workload is large, length consuming time, is not a kind of method of economical and efficient.The microorganism enrichment of using biological stephanoporate filler to realize in pipe network water is a kind of feasible program.In practical application, biologic packing material is generally used in sewage disposal, and microorganic adhesion is at filling surface, effluent stream when filler, by bacteria metabolism effect, the pollution substance in degradation of sewage.In water supply network, using biologic packing material, is mainly to utilize filler to be easy to the feature of microorganic adhesion, reaches the object of microorganism in enrichment water supply network pipe network water.
Summary of the invention
Object of the present invention is exactly to provide a kind of assembling simple in order to overcome the defect of above-mentioned prior art existence, microorganism enrichment sampling unit in water supply network pipe network water easy to maintenance.
Object of the present invention can be achieved through the following technical solutions: microorganism enrichment sampling unit in a kind of water supply network pipe network water, this device comprises water service pipe, it is characterized in that, also comprise rigidity rope, sleeve pipe, protruded packing, graticule mesh, gasket seal, described sleeve pipe and protruded packing are intervally arranged, and form filler string by rigidity rope string together, this filler string penetrates in water service pipe, and rigidity rope two ends are fixed in the graticule mesh that is placed in water service pipe end, water service pipe end and adjacent tubular segments are close to clamps graticule mesh, and the upper gasket seal of pad is connected and fixed.
Described water service pipe is connected by clip or flange with adjacent tubular segments.Water service pipe caliber and research pipeline section caliber, material are all identical, and its length can need conversion according to research.
Described clip is stuck in the junction of water service pipe and adjacent tubular segments, and fixes by bolt and nut.
Described rigidity rope requires to have certain rigidity, and diameter is unsuitable excessive, and diameter is 1～3mm, and the centre hole through protruded packing, strings sleeve pipe, protruded packing successively, and two ends are fixed in the graticule mesh of water service pipe end face, in order to fixing protruded packing.
Described sleeve pipe is hollow type sleeve pipe, and its length is greater than 1 times of protruded packing diameter, is less than 3 times of protruded packing diameters, and its internal diameter is greater than described rigidity rope diameter and is less than the aperture of protruded packing.
Described protruded packing is ring, for increasing microbial film bond area, realizes biomembranous enrichment in water service pipe, before putting into water service pipe, protruded packing is carried out to high-temperature sterilization, guarantees protruded packing surface sterile, prevents from bringing miscellaneous bacteria into tubing system.
Described graticule mesh is arranged on water service pipe front and back end, and its grid gap is less than the external diameter of protruded packing, and its diameter is greater than water service pipe internal diameter, is less than or equal to clamp connection place diameter of section.
In described water supply network pipe network water, microorganism enrichment sampling unit two ends are equipped with gate valve, closing gate valve during protruded packing sampling.
The material of described rigidity rope, sleeve pipe, protruded packing, graticule mesh is inert material, before putting into water service pipe, all carries out sterilization.
Microorganism enrichment sampling unit in described water supply network pipe network water, in actual applications, is divided into assembling and two kinds of situations of sampling.In assembling water supply network pipe network water during microorganism enrichment sampling unit, first rigidity rope is passed to protruded packing, the sleeve pipe of sterilization successively, finally the two ends of rigidity rope are lain in graticule mesh, graticule mesh is fixed on clamp connection place at water service pipe two ends, cover front band, after tightening nuts, complete microorganism enrichment sampling unit assembling in described water supply network pipe network water.Open gate valve, device brings into operation.
In described water supply network pipe network water, microorganism enrichment sampling unit is when sampling, first want stopping device two ends gate valve, open water service pipe two ends clip, take off water service pipe, untie the rigidity rope being fixed in the graticule mesh of one end, from the other end, protruded packing string is taken out, take protruded packing away, complete sampling, re-assemblied as required microorganism enrichment sampling unit in water supply network pipe network water.
Compared with prior art, feature of the present invention and beneficial effect:
1. in water supply network pipe network water of the present invention, microorganism enrichment sampling unit is simple in structure, easy to loading and unloading.
2. microorganism enrichment sampling unit in a kind of water supply network pipe network water of the present invention, protruded packing is evenly distributed, and is beneficial to microorganism enrichment.
3. microorganism enrichment sampling unit in a kind of water supply network pipe network water of the present invention, protruded packing position is fixed, and is not subject to water currents.
4. in a kind of water supply network pipe network water of the present invention, microorganism enrichment sampling unit adopts protruded packing, has increased the contact area of carrier and water, can realize fast the enrichment of microorganism in pipe network water.
Accompanying drawing explanation
Fig. 1 is one-piece construction schematic diagram of the present invention;
Fig. 2 is a-a sectional view.
Mainly comprise the protruded packing string being comprised of rigidity rope 1, sleeve pipe 2, protruded packing 3, water service pipe 4, clip 5, gate valve 6, nut 7, bolt 8, graticule mesh 9 and gasket seal 10 form.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in detail.
Embodiment 1
As shown in Fig. 1～2, microorganism enrichment sampling unit in a kind of water supply network pipe network water, this device comprises water service pipe 4, also comprise rigidity rope 1, sleeve pipe 2, protruded packing 3, graticule mesh 9, gasket seal 10, described sleeve pipe 2 and protruded packing 3 are intervally arranged, and form filler string by rigidity rope 1 string together, this filler string penetrates in water service pipe 4, and rigidity rope 1 two ends are fixed in the graticule mesh 9 that is placed in water service pipe 4 ends, water service pipe 4 ends and adjacent tubular segments are close to clamps graticule mesh 9, and the upper gasket seal 10 of pad is connected and fixed.
Described water service pipe 4 is connected by clip 5 with adjacent tubular segments.Water service pipe caliber and research pipeline section caliber, material are all identical, and its length can need conversion according to research.Described clip 5 is stuck in the junction of water service pipe 4 and adjacent tubular segments, and fixing by bolt 8 and nut 7.
Described rigidity rope 1 requires to have certain rigidity, and diameter is unsuitable excessive, and diameter is 1～3mm, and the centre hole through protruded packing, strings sleeve pipe 2, protruded packing 3 successively, and two ends are fixed in the graticule mesh 9 of water service pipe 4 end faces, in order to fixing protruded packing.
Described sleeve pipe 2 is hollow type sleeve pipe, and its length is greater than 1 times of protruded packing diameter, is less than 3 times of protruded packing diameters, and its internal diameter is greater than described rigidity rope diameter and is less than the aperture of protruded packing.
Described protruded packing 3 is ring, for increasing microbial film bond area, realizes biomembranous enrichment in water service pipe, before putting into water service pipe 4, protruded packing is carried out to high-temperature sterilization, guarantees protruded packing surface sterile, prevents from bringing miscellaneous bacteria into tubing system.
Described graticule mesh 9 is arranged on water service pipe 4 front and back ends, and its grid gap is less than the external diameter of protruded packing 3, and its diameter is greater than water service pipe 4 internal diameters, is less than or equal to clip 5 junction diameter of sections.
In described water supply network pipe network water, microorganism enrichment sampling unit two ends are equipped with gate valve 6, closing gate valve 6 during protruded packing 3 sampling.
The material of described rigidity rope 1, sleeve pipe 2, protruded packing 3, graticule mesh 9 is inert material, before putting into water service pipe 4, all carries out sterilization.
Microorganism enrichment sampling unit in described water supply network pipe network water, in actual applications, is divided into assembling and two kinds of situations of sampling.In assembling water supply network pipe network water during microorganism enrichment sampling unit, first rigidity rope is passed to protruded packing, the sleeve pipe of sterilization successively, finally the two ends of rigidity rope are lain in graticule mesh, graticule mesh is fixed on clamp connection place at water service pipe two ends, cover front band, after tightening nuts, complete microorganism enrichment sampling unit assembling in described water supply network pipe network water.Open gate valve, device brings into operation.
In described water supply network pipe network water, microorganism enrichment sampling unit is when reality samples, first want stopping device two ends gate valve, open water service pipe two ends clip, take off water service pipe, untie the rigidity rope being fixed in the graticule mesh of one end, from the other end, protruded packing string is taken out, take protruded packing away, complete sampling, re-assemblied as required microorganism enrichment sampling unit in water supply network pipe network water.
In practical application, first according to above-mentioned steps, carry out the assembling of microorganism enrichment sampling unit in water supply network pipe network water.
In the described water supply network pipe network water of assembling during microorganism enrichment sampling unit, first rigidity rope 1 is passed to sleeve pipe 2 and protruded packing 3 successively, after the number that meets required protruded packing 3 requires, protruded packing string is penetrated in water service pipe 4, the end of rigidity rope 1 is fixed in the graticule mesh 9 that is placed in water service pipe 4 ends, water service pipe 4 ends and adjacent tubular segments are close to, clamp graticule mesh 9, on pad, gasket seal 10, card front band 5, at clip interface, plug bolt 8, tightening nuts 7, completes microorganism enrichment sampling unit assembling in described water supply network pipe network water.
After having assembled, open the gate valve 6 at microorganism enrichment sampling unit two ends in described water supply network pipe network water, according to testing program, running gear.After running to the scheduled time, can carry out sampling work.
While sampling work, first close the gate valve 6 at microorganism enrichment sampling unit two ends in described water supply network pipe network water, unscrew afterwards nut 7, take off clip 5, slowly put the water in clean water service pipe 4, then untie the rigidity rope 1 being fixed in graticule mesh 9, protruded packing string is slowly taken out from one end of water service pipe 4 simultaneously, then take required protruded packing 3 away, protruded packing has sampled.According to test needs, microorganism enrichment sampling unit in the described water supply network pipe network water of recombinating, reruns testing apparatus.
Microorganism enrichment sampling unit in described a kind of water supply network pipe network water is applied in test and is found, operation through 4 months, after protruded packing is sampled, use ultrasonator that the microorganism on protruded packing is shaken in solution, then adopt test kit to extract the DNA in solution, the concentration of measuring the DNA sample of carrying reaches 10ng/ μ L left and right.In described a kind of water supply network pipe network water, microorganism enrichment sampling unit can reach the object of the microorganism in enrichment pipe network water, is a kind of efficient method.
Embodiment 2
Shown in Fig. 1～2, described water service pipe 4 is connected by clip or flange with adjacent tubular segments.All the other are with embodiment 1.
Claims (9)
1. microorganism enrichment sampling unit in a water supply network pipe network water, this device comprises water service pipe (4), it is characterized in that, also comprise rigidity rope (1), sleeve pipe (2), protruded packing (3), graticule mesh (9), gasket seal (10), described sleeve pipe (2) and protruded packing (3) are intervally arranged, and form filler string by rigidity rope (1) string together, this filler string penetrates in water service pipe (4), and rigidity rope (1) two ends are fixed in the graticule mesh (9) that is placed in water service pipe (4) end, water service pipe (4) end and adjacent tubular segments are close to clamps graticule mesh (9), and the upper gasket seal (10) of pad is connected and fixed.
2. microorganism enrichment sampling unit in water supply network pipe network water according to claim 1, is characterized in that, described water service pipe (4) is connected by clip (5) or flange with adjacent tubular segments.
3. microorganism enrichment sampling unit in water supply network pipe network water according to claim 2, it is characterized in that, described clip (5) is stuck in the junction of water service pipe (4) and adjacent tubular segments, and fixing by bolt (8) and nut (7).
4. microorganism enrichment sampling unit in water supply network pipe network water according to claim 1, it is characterized in that, described rigidity rope (1) diameter is 1～3mm, centre hole through protruded packing, sleeve pipe (2), protruded packing (3) are strung successively, two ends are fixed in the graticule mesh (9) of water service pipe (4) end face, and protruded packing position is fixed, and is not subject to water currents.
5. microorganism enrichment sampling unit in water supply network pipe network water according to claim 1, it is characterized in that, described sleeve pipe (2) is hollow type sleeve pipe, its length is greater than 1 times of protruded packing diameter, be less than 3 times of protruded packing diameters, its internal diameter is greater than described rigidity rope diameter and is less than the aperture of protruded packing.
6. microorganism enrichment sampling unit in water supply network pipe network water according to claim 1, is characterized in that, described protruded packing (3) is ring, puts into water service pipe (4) before, and protruded packing is carried out to high-temperature sterilization.
7. microorganism enrichment sampling unit in water supply network pipe network water according to claim 3, it is characterized in that, described graticule mesh (9) is arranged on water service pipe (4) front and back end, its grid gap is less than the external diameter of protruded packing (3), its diameter is greater than water service pipe (4) internal diameter, is less than or equal to clip (5) junction diameter of section.
8. microorganism enrichment sampling unit in water supply network pipe network water according to claim 1, it is characterized in that, in described water supply network pipe network water, microorganism enrichment sampling unit two ends are equipped with gate valve (6), closing gate valve (6) during protruded packing (3) sampling.
9. microorganism enrichment sampling unit in water supply network pipe network water according to claim 1, it is characterized in that, the material of described rigidity rope (1), sleeve pipe (2), protruded packing (3), graticule mesh (9) is inert material, put into water service pipe (4) before, all carry out sterilization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410191554.4A CN103966080B (en) | 2014-05-08 | 2014-05-08 | Microorganism enrichment sampler in a kind of water supply network pipe network water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410191554.4A CN103966080B (en) | 2014-05-08 | 2014-05-08 | Microorganism enrichment sampler in a kind of water supply network pipe network water |
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| Publication Number | Publication Date |
|---|---|
| CN103966080A true CN103966080A (en) | 2014-08-06 |
| CN103966080B CN103966080B (en) | 2016-08-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410191554.4A Expired - Fee Related CN103966080B (en) | 2014-05-08 | 2014-05-08 | Microorganism enrichment sampler in a kind of water supply network pipe network water |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107400699A (en) * | 2017-07-13 | 2017-11-28 | 上海市水利工程设计研究院有限公司 | A kind of pipeline biomembrane sampling system and the method for sampling based on driven by power |
| CN109030088A (en) * | 2018-08-15 | 2018-12-18 | 中冶华天工程技术有限公司 | Microorganism enrichment sampler in situ and method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2248585Y (en) * | 1996-04-02 | 1997-03-05 | 林云明 | Biological filtering material for waste water treatment |
| CN1215029A (en) * | 1997-10-21 | 1999-04-28 | 中国石化扬子石油化工公司 | Anaerobic reation pool and method for treating waste water |
| CN102674546A (en) * | 2012-05-29 | 2012-09-19 | 东南大学 | Two-stage anaerobic reactor and method suitable for blue-green algae fermentation |
| CN202625912U (en) * | 2012-04-12 | 2012-12-26 | 廊坊恒盛环保设备有限公司 | Carbon fiber biological filler combined structure |
-
2014
- 2014-05-08 CN CN201410191554.4A patent/CN103966080B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2248585Y (en) * | 1996-04-02 | 1997-03-05 | 林云明 | Biological filtering material for waste water treatment |
| CN1215029A (en) * | 1997-10-21 | 1999-04-28 | 中国石化扬子石油化工公司 | Anaerobic reation pool and method for treating waste water |
| CN202625912U (en) * | 2012-04-12 | 2012-12-26 | 廊坊恒盛环保设备有限公司 | Carbon fiber biological filler combined structure |
| CN102674546A (en) * | 2012-05-29 | 2012-09-19 | 东南大学 | Two-stage anaerobic reactor and method suitable for blue-green algae fermentation |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107400699A (en) * | 2017-07-13 | 2017-11-28 | 上海市水利工程设计研究院有限公司 | A kind of pipeline biomembrane sampling system and the method for sampling based on driven by power |
| CN107400699B (en) * | 2017-07-13 | 2024-03-15 | 上海市水利工程设计研究院有限公司 | Pipeline biological film sampling system and sampling method based on electric drive |
| CN109030088A (en) * | 2018-08-15 | 2018-12-18 | 中冶华天工程技术有限公司 | Microorganism enrichment sampler in situ and method |
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| CN103966080B (en) | 2016-08-24 |
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Granted publication date: 20160824 Termination date: 20190508 |