CN112280680A - 用于特异性分离目标核酸的方法 - Google Patents
用于特异性分离目标核酸的方法 Download PDFInfo
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- CN112280680A CN112280680A CN202011208048.3A CN202011208048A CN112280680A CN 112280680 A CN112280680 A CN 112280680A CN 202011208048 A CN202011208048 A CN 202011208048A CN 112280680 A CN112280680 A CN 112280680A
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- cholesterol
- solution
- saponin
- microorganism
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Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR1350650 | 2013-01-25 | ||
| FR1350650A FR3001464B1 (fr) | 2013-01-25 | 2013-01-25 | Procede d'isolement specifique d'acides nucleiques d'interet |
| CN201480005790.0A CN104955948A (zh) | 2013-01-25 | 2014-01-27 | 用于特异性分离目标核酸的方法 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201480005790.0A Division CN104955948A (zh) | 2013-01-25 | 2014-01-27 | 用于特异性分离目标核酸的方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112280680A true CN112280680A (zh) | 2021-01-29 |
Family
ID=48613751
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011208048.3A Pending CN112280680A (zh) | 2013-01-25 | 2014-01-27 | 用于特异性分离目标核酸的方法 |
| CN201480005790.0A Pending CN104955948A (zh) | 2013-01-25 | 2014-01-27 | 用于特异性分离目标核酸的方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201480005790.0A Pending CN104955948A (zh) | 2013-01-25 | 2014-01-27 | 用于特异性分离目标核酸的方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US9944974B2 (enExample) |
| EP (2) | EP2948548B2 (enExample) |
| JP (3) | JP6533468B2 (enExample) |
| CN (2) | CN112280680A (enExample) |
| DK (1) | DK2948548T4 (enExample) |
| ES (2) | ES2752014T5 (enExample) |
| FR (1) | FR3001464B1 (enExample) |
| WO (1) | WO2014114896A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN120118978A (zh) * | 2025-05-07 | 2025-06-10 | 兰州百源基因技术有限公司 | 一种用于白色念珠菌的裂解液、试剂盒及基因检测方法 |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3001464B1 (fr) | 2013-01-25 | 2016-02-26 | Biomerieux Sa | Procede d'isolement specifique d'acides nucleiques d'interet |
| WO2016183012A1 (en) * | 2015-05-11 | 2016-11-17 | 3M Innovative Properties Company | Composition for reducing inhibition of nucleic acid amplification |
| WO2017080714A1 (en) * | 2015-11-11 | 2017-05-18 | Siemens Healthcare Diagnostics Inc. | Method of processing nucleic acids |
| WO2017118719A1 (en) * | 2016-01-08 | 2017-07-13 | Pathoquest | Modulation of accessibility of host nucleic acids to nucleic acid digesting enzymes in a cellular biological fluids |
| JP2019507588A (ja) * | 2016-01-08 | 2019-03-22 | パスクエスト | 無細胞体液中の核酸消化酵素に対する宿主核酸の到達性の調整 |
| CA3022947A1 (en) * | 2016-05-04 | 2017-11-09 | Children's Hospital & Research Center At Oakland | Rapid extraction of nucleic acids from clinical samples for downstream applications |
| CN109923217B (zh) | 2016-10-13 | 2023-06-16 | 生物梅里埃公司 | 宏基因组样品中病原体的鉴定和抗生素表征 |
| JP7116389B2 (ja) * | 2018-07-03 | 2022-08-10 | Ｋｔｅｎ Ｂｉｏ株式会社 | ウイルス濃縮方法 |
| JP7338134B2 (ja) * | 2018-09-07 | 2023-09-05 | Ｋｔｅｎ Ｂｉｏ株式会社 | ウイルス消毒効果判定方法及び該判定方法により選定された抗ウイルス消毒剤 |
| CN109055360B (zh) * | 2018-09-14 | 2022-10-11 | 皖南医学院 | 一种提取按蚊总rna的方法 |
| CN109401975B (zh) * | 2018-11-28 | 2021-08-03 | 云南中烟工业有限责任公司 | 一种收集陈化烟叶表面微生物的方法 |
| CN112301097B (zh) * | 2019-07-26 | 2022-10-21 | 申翌生物科技(杭州)有限公司 | 样本裂解和pcr反应组合物 |
| WO2021000750A1 (zh) | 2019-07-01 | 2021-01-07 | 申翌生物科技(杭州)有限公司 | 利用pcr综合反应系统进行pcr反应的新方法 |
| FR3099183B1 (fr) | 2019-07-23 | 2022-11-18 | Biomerieux Sa | Procédé de détection et de quantification d'une espèce biologique d'intérêt par analyse métagénomique, et détermination d'un niveau de confiance associé |
| FR3099181B1 (fr) | 2019-07-23 | 2022-11-18 | Biomerieux Sa | Procédé de détection et de quantification d'une espèce biologique d'intérêt par analyse métagénomique, avec prise en compte d'un calibrateur. |
| FR3099180B1 (fr) | 2019-07-23 | 2022-11-25 | Biomerieux Sa | Procédé de détection et de quantification d'une espèce biologique d'intérêt par analyse métagénomique, comportant l'utilisation d'une espèce de contrôle. |
| FR3099182B1 (fr) | 2019-07-23 | 2022-11-25 | Biomerieux Sa | Procédé de détection et de quantification d'une espèce biologique d'intérêt par analyse métagénomique |
| CN112813137A (zh) * | 2019-11-15 | 2021-05-18 | 西安中科茵康莱医学检验有限公司 | 一种液体生物样品中选择性去除宿主核酸的方法 |
| CN111662959A (zh) * | 2020-07-22 | 2020-09-15 | 中国医学科学院病原生物学研究所 | 一种真菌高通量快速检测方法 |
| EP4341420A1 (en) * | 2021-05-19 | 2024-03-27 | Russom, Amam | Selective lysis of mammalian eukaryotic cells and visualization of viable bacterial cells |
| FR3130291B1 (fr) | 2021-12-15 | 2025-05-02 | Biomerieux Sa | Procédé de détection d’une présence d’une espèce biologique d’intérêt par séquençage temps réel itératif. |
| EP4574991A1 (fr) * | 2023-12-18 | 2025-06-25 | bioMérieux | Méthode pour détecter des microorganismes viables potentiellement présents dans un échantillon de produits cellulaires |
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| US20150337362A1 (en) | 2015-11-26 |
| EP3578654A1 (fr) | 2019-12-11 |
| EP2948548B2 (fr) | 2022-11-23 |
| CN104955948A (zh) | 2015-09-30 |
| ES2752014T5 (es) | 2023-04-10 |
| EP3578654B1 (fr) | 2025-04-09 |
| ES2752014T3 (es) | 2020-04-02 |
| JP2019068818A (ja) | 2019-05-09 |
| FR3001464B1 (fr) | 2016-02-26 |
| ES3025783T3 (en) | 2025-06-09 |
| EP2948548A1 (fr) | 2015-12-02 |
| JP2021019600A (ja) | 2021-02-18 |
| JP6533468B2 (ja) | 2019-06-19 |
| FR3001464A1 (fr) | 2014-08-01 |
| WO2014114896A1 (fr) | 2014-07-31 |
| EP2948548B1 (fr) | 2019-07-31 |
| DK2948548T3 (da) | 2019-11-11 |
| JP6793709B2 (ja) | 2020-12-02 |
| US9944974B2 (en) | 2018-04-17 |
| EP3578654C0 (fr) | 2025-04-09 |
| JP2016505273A (ja) | 2016-02-25 |
| DK2948548T4 (da) | 2023-02-20 |
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