CN112159274A

CN112159274A - Microbial granular inoculant with biocontrol nutrition function

Info

Publication number: CN112159274A
Application number: CN202011044910.1A
Authority: CN
Inventors: 郑安科; 张俊庆; 孔云; 田帅; 刘海鹏; 王越
Original assignee: Shandong Tumuqi Biotechnology Co ltd
Current assignee: Shandong Tumuqi Biotechnology Co ltd
Priority date: 2020-09-28
Filing date: 2020-09-28
Publication date: 2021-01-01

Abstract

The invention discloses a microbial granular microbial inoculum with a biocontrol nutrition function, and mainly relates to the field of microbial inoculum granular fertilizer. The composite fermentation substrate comprises, by weight, 55-75 parts of a granular core layer, 5-35 parts of a middle cladding layer and 5-25 parts of an outer cladding layer which are sequentially coated from inside to outside, wherein the raw material of the granular core layer comprises a product obtained by composite fermentation of lactobacillus plantarum and bacillus subtilis on an organic fermentation substrate; the raw materials of the middle cladding layer comprise a mixed fermentation product of bacillus mucilaginosus and bacillus subtilis and a synergist containing 2- (3, 4-dichlorophenoxy) triethylamine; the outer coating contains calcium silicon fertilizer containing active calcium silicon. The invention has better biocontrol nutrition effect, can effectively compensate soil probiotics, improve the physical and chemical properties of soil, prevent soil-borne diseases, release fertilizer efficiency layer by layer, have slow release effect, improve the stress resistance of crops, promote the growth of crops and improve the taste of the crops.

Description

Microbial granular inoculant with biocontrol nutrition function

Technical Field

The invention relates to the field of microbial inoculum granular fertilizer, in particular to a microbial inoculum with biocontrol nutrition function.

Background

Chemical fertilizers are important agricultural production materials, and are 'grains' of grains. The fertilizer plays an irreplaceable role in promoting the development of grain and agricultural production, but the problems of excessive application, blind application and the like of the fertilizer exist at present, so that the cost is increased and the environment is polluted, the improvement of a fertilizing mode is urgently needed, the utilization rate of the fertilizer is improved, the unreasonable investment is reduced, the effective supply of main agricultural products such as grain and the like is ensured, and the agricultural sustainable development is promoted. Therefore, the country and the society advocate negative increase of the use amount more since 2020, and put forward higher requirements on the industry, and because the use amount of the fertilizer needs to be controlled, zero increase and negative increase are realized, the fertilizer quality needs to inherit sustainable development, improve quality and efficiency, improve the safety of the fertilizer for crops, and seek breakthrough in the aspect of organic nutrition.

Disclosure of Invention

The invention aims to provide a microbial granular inoculant with biocontrol nutrition function, which has better biocontrol effect, releases fertilizer efficiency layer by layer, increases soil fertility and drought resistance, promotes crop growth and improves crop taste.

In order to achieve the purpose, the invention is realized by the following technical scheme:

a microbial granular bacterial agent with biocontrol nutrition function comprises 55-75 parts of a granular core layer, 5-35 parts of a middle cladding layer and 5-25 parts of an outer cladding layer which are sequentially coated from inside to outside in parts by weight,

the raw material of the granular core layer comprises a product obtained by blending and fermenting an organic fermentation substrate by lactobacillus plantarum and bacillus subtilis;

the raw materials of the middle cladding layer comprise a mixed fermentation product of bacillus mucilaginosus and bacillus subtilis and 2- (3, 4-dichlorophenoxy) triethylamine;

the outer coating contains calcium silicon fertilizer containing active calcium silicon.

Further, the core layer comprises the following raw materials in parts by weight:

20 to 45 portions of mineral fulvic acid with 20 to 70 percent of humic acid, 20 to 60 portions of biochemical fulvic acid with 60 to 80 percent of organic matter, 5 to 25 portions of amino acid solution, 5 to 15 portions of lactobacillus plantarum fermentation stock solution and 0.5 to 0.8 portion of bacillus subtilis fermentation solution.

Further, the processing technology of the particle core layer comprises the following steps:

feeding mineral fulvic acid with humic acid content of 20-70% and biochemical fulvic acid with organic matter content of 60-80% in a mixing tank according to the proportion of 1: 1-2;

adding amino acid solution accounting for 5-25 parts of the total mass of the particle core layer, wherein the content of free amino acid is 5-10%;

adding lactobacillus plantarum fermentation stock solution accounting for 5-15 parts of the total mass of the granular core layer, wherein the pH is 3.5-4.5, the content of lactobacillus is more than 0.1 hundred million/ml, and uniformly mixing to control the water content to be 45-55%;

5-8Kg of bacillus subtilis fermentation broth with the bacterium content of more than 100 hundred million/ml is added per ton;

fermenting under natural conditions, wherein in the fermentation process, stirring is carried out for 32-36 hours for the first time and 1min at the rotating speed of 80 rpm, then stirring is carried out for 1min every 6-8 hours, and the fermentation is completed for 5-10 days to obtain the grain core layer ingredient;

and (3) carrying out ammoniation granulation on the ingredients of the particle core layer, drying at the high temperature of 220-280 ℃, and carrying out sieving treatment, wherein the diameter of the screened particles is 2-3.5mm, and the water content is less than or equal to 25 parts, so as to obtain particles of the particle core layer.

Further, the raw materials of the middle cladding layer comprise the following components in parts by weight: 45-75 parts of enzymolysis feather powder containing more than or equal to 40 percent of free amino acid, 15-25 parts of soybean powder containing more than or equal to 30 percent of protein, 5-10 parts of 100 hundred million/g of bacillus mucilaginosus, 3-5 parts of 1000 hundred million/g of bacillus subtilis and 0.025-0.125 part of 2- (3, 4-dichlorophenoxy) triethylamine.

Further, the processing technology of the middle cladding layer comprises the following steps:

mixing materials, namely mixing and uniformly stirring enzymolysis feather powder containing more than or equal to 40 percent of free amino acid and soybean powder containing more than or equal to 30 percent of protein;

adding a microbial inoculum, namely adding bacillus mucilaginosus and bacillus subtilis into the amino acid substrate to ensure that the total viable count of the bacillus subtilis and the bacillus mucilaginosus is 0.2-15 hundred million/g in the whole particles;

adding 2- (3, 4-dichlorophenoxy) triethylamine, and mixing uniformly to obtain a middle layer ingredient;

adding the mixed middle layer ingredients into a mixing tank, uniformly stirring, and coating the mixture on the particle core layer particles through a disc for secondary low-temperature coating to form a coating layer with the thickness of 0.5-1.5 mm. The diameter of the particles of the intermediate coating is between 2.5 and 4.5mm, and the water content is less than or equal to 20 percent.

Further, the raw material of the envelope is obtained by the following method: uniformly mixing active silicon and 0.1-0.15% citric acid aqueous solution according to the mass ratio of 1: 2-2.5; adding starch 3-5% of the total amount of the outer cladding layer, polyaspartic acid 0.5-1% of the total amount of the outer cladding layer, and calcium carbonate 2-5% of the total amount of the outer cladding layer into the mixture, and stirring to obtain a suspension to obtain an outer layer guniting ingredient.

Further, the active silicon comprises citrate soluble silicon and water soluble silicon in a ratio of 8: 2-9.5: 0.5.

Further, the processing method of the outer cladding comprises the following steps: and (3) using a guniting granulation method, uniformly attaching the three cladding ingredients to the surface of the middle cladding in a guniting mode, drying at the temperature of 80-110 ℃, and sieving to ensure that the particle diameter is between 3-5mm and the whole water content is less than 25%.

Furthermore, the finished product of the microbial inoculum particles contains 40-60% of organic matters, 5-20% of protein, 10-40% of humic acid and 0.2-15 hundred million/g of bacteria.

Compared with the prior art, the invention has the beneficial effects that:

the outer cladding layer contains active silicon and calcium elements, is released in soil firstly, can improve the quality of crops, provides a calcium with slower solubility, provides nutrient components, and can effectively protect the activity of strains in the middle layer; the high-activity strain of the middle cladding layer achieves the effect of self-carried carbon nitrogen nutrient substances by attaching protein and the fulvic acid outer starch of the inner interlayer, has better adaptability, stronger activity and better planting effect, has larger population advantage after being used and customized in soil, can quickly decompose and release metabolites, and the middle layer is compounded with two kinds of bacillus, can effectively inhibit the occurrence of soil-borne diseases, prevents fungi and bacterial diseases caused by the soil-borne diseases, plays a better biological control effect, and simultaneously increases the bacillus mucilaginosus for improving the utilization rate of nutrient elements on the basis of biological control; the granular core layer contains high-content organic matters, can be quickly decomposed into humus to improve the utilization rate of the organic matters, provides nutrients and various trace elements for soil, and can effectively stimulate the growth of capillary roots of crops, increase the absorption of nutrient components and improve the taste of the crops.

Drawings

FIG. 1 is a photograph of a product of the microbial inoculum particles of the invention;

FIG. 2 is a photograph showing the results of comparative experiments in example 4 (the results of 12 days of treatment, left control group and right experimental group).

Detailed Description

The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and these equivalents also fall within the scope of the present application.

The instruments, reagents, materials and the like used in the following examples are conventional instruments, reagents, materials and the like in the prior art and are commercially available in a normal manner unless otherwise specified. Unless otherwise specified, the experimental methods, detection methods, and the like described in the following examples are conventional experimental methods, detection methods, and the like in the prior art.

Example 1: microbial granular inoculant with biocontrol nutrition function

The granular microbial inoculum is a granular material, the diameter of the granules is 3-5mm, the granular microbial inoculum is composed of three layers, and the three colors are black, gray and white from inside to outside respectively.

The hierarchical structure is formed mainly by three layers of granulation processes, including a grain core layer, a middle cladding layer and an outer cladding layer; the pellet is prepared by sequentially carrying out granulation and coating processes from a pellet core layer, and the specific processes are as follows:

1. granule core layer

The pellet core layer is 65% of the whole pellet by weight, and the raw materials of the pellet core layer comprise the following components in parts by weight:

30 parts of mineral source fulvic acid with 55% of humic acid, 55 parts of biochemical fulvic acid with 65% of organic matter content, 15 parts of amino acid solution, 10 parts of lactobacillus plantarum fermentation stock solution and 6Kg of bacillus subtilis fermentation broth per ton;

the process comprises the following steps:

1.1 feeding mineral fulvic acid with 55 percent of humic acid and biochemical fulvic acid with 65 percent of organic matter in a proportioning pool in proportion;

1.2, adding amino acid solution, wherein the content of free amino acid is 5.3%;

1.3 adding lactobacillus plantarum fermentation stock solution, wherein the pH is 3.65, the content of lactobacillus is 0.18 hundred million/ml, and the water content is 48 percent after uniformly mixing;

1.4 per ton of bacillus subtilis fermentation liquor 8Kg with the bacteria content of 188 hundred million/ml;

1.5 fermenting under natural conditions, stirring for 1min after 34 hours for the first time in the fermentation process, stirring for 1min every 8 hours later, and completing fermentation for 8 days to obtain a grain core layer ingredient;

1.6 ammoniating and granulating the ingredients of the core layer, drying at the high temperature of 250-260 ℃, and sieving to obtain the core layer particles with the particle diameter of 2-3.5mm and the moisture of 23%.

The granule core layer mainly comprises amino acid liquid which is formed by mixing humic acid and 21 amino acids, wherein the amino acid mainly acts as nitrogen fertilizer in an amino acid state, the agricultural amino acid mainly comprises yeast and monosodium glutamate leftovers, and a plurality of plant stimulants are generated in the fermentation process and can effectively stimulate the growth of crops (the stimulants have complex components and are not clear), are aminated and granulated, and are dried at high temperature. The organic matter and the nitrogen for crop growth are provided, the soil structure can be effectively improved, the buffering effect of the soil on adverse conditions is increased, and the root growth is promoted;

2. middle cladding

The weight of the raw materials of the middle coating layer accounts for 18 percent of the whole granule, and the raw materials of the middle coating layer comprise the following components in parts by weight:

60 parts of enzymolysis feather powder containing 40 percent of free amino acid, 8 parts of soybean meal containing 35 percent of protein, 5 parts of 100 hundred million/g bacillus mucilaginosus, 5 parts of 1000 hundred million/g bacillus subtilis and 0.085 part of 2- (3, 4-dichlorophenoxy) triethylamine synergist

2.1 mixing materials, namely mixing and stirring evenly enzymolysis feather powder containing 40 percent of free amino acid and soybean meal containing 35 percent of protein;

2.2 adding a microbial inoculum, namely adding bacillus mucilaginosus and bacillus subtilis into an amino acid substrate to ensure that the total viable count of the bacillus subtilis and the bacillus mucilaginosus is 0.2-15 hundred million/g in the whole granule;

2.3 adding 2- (3, 4-dichlorophenoxy) triethylamine; the 2- (3, 4-dichlorophenoxy) triethylamine directly acts on plant cell nucleuses, can influence plant gene transcription or translation, repair incomplete genes of plants, and induce dominant gene expression; on the other hand, the compound enzyme preparation plays a role in improving the activity of various enzymes in a plant body, increasing the contents of chlorophyll, protein and amino acid, enhancing the photosynthesis of the plant, promoting the division and growth of plant cells and the carbon and nitrogen metabolism of the plant, and regulating the balance of nutrients in the body. The method has the characteristics of no residue and good safety. 2. Has quick-acting and lasting effect and wider application. 3. The fertilizer can be used in the whole growth period, the yield and the quality can be increased by 4, the photosynthesis can be promoted, the chlorophyll decomposition is delayed by 5, the enzyme activity is increased, the accumulation of carbohydrate, protein and lipid substances is promoted, the swelling and coloring of fruits is promoted, the yield is effectively increased, the premature senility is prevented, the quality is improved, and the fertilizer is mainly called as a quality improving expert 6 by American planting, the primary metabolism and the secondary metabolism are promoted, the synthesis and the accumulation of active substances are promoted, and the detailed resistance of plants is enhanced.

2.4 adding the mixed middle layer ingredients into a batching pool, uniformly stirring, and coating the mixture on the particle core layer particles through a disc for secondary low-temperature coating to form a coating layer with the thickness of 0.5-1.5 mm. The diameter of the particles of the intermediate coating is between 2.5 and 4.5mm, and the moisture content is 18 percent.

The middle layer is protein material of enzymolysis amino acid powder, compounded with high activity hay, jelly-like bacteria powder, and coated twice at low temperature. The survival rate of the strains is ensured, the stability of the strains is improved, and the protein material provides nutrient substances for the strains.

3. Outer cladding

Accounting for 17% by weight of the whole pellet.

3.1 the raw material of the envelope is obtained by the following method: adding organic silicon, wherein the ratio of citric-soluble silicon to water-soluble silicon is 9:1, adding active silicon into 0.1% citric acid aqueous solution according to the mass ratio of 1:2.1, and uniformly mixing; adding 4% of starch, 0.8% of polyaspartic acid and 3.5% of calcium carbonate into the mixture, and stirring to obtain a suspension to obtain the outer-layer guniting ingredient.

3.2 the processing method of the outer layer comprises the following steps: uniformly attaching the granules to the surface layer of the granules by a guniting mode, drying at the temperature of 80-110 ℃, and sieving to ensure that the diameter of the granules is 3-5mm and the whole water content is 18.6%.

The outer cladding layer is made of bioactive silicon and is granulated by spraying. Supplementing nutrient elements: the silicon fertilizer is a quality fertilizer, can obviously improve the quality of agricultural products, effectively prevent fruit cracking, fruit shrinking and malformed fruits, increase the hardness of the fruits, enable the fruits to have regular shapes, good coloring, good taste, good marketability, storage and transportation resistance and prolong the preservation period.

The polyaspartic acid is a water-soluble biomimetic polymer, and is used as a plant nutrient absorption promoter in agriculture, so that the absorption of plants on nutrient elements can be effectively promoted, the agricultural production cost is reduced, the yield and the quality of crops are improved, and the pollution of surface water and underground water caused by fertilizer and pesticide loss is reduced; it can be used with pesticide and fertilizer, and can raise the utilization rate of pesticide and fertilizer and raise fertilizer competitiveness.

The finished product of the microbial inoculum particles prepared by the process contains 45 percent of organic matters, 18.2 percent of protein, 26.5 percent of humic acid and 9.9 hundred million/g of bacteria.

The microbial inoculum particles are rich in high-activity bacillus, can effectively inhibit the occurrence of soil-borne diseases, prevent fungi and bacterial diseases caused by the soil-borne diseases, and play a good biological control effect.

In the aspect of nutrition, the microbial inoculum particles are rich in organic matters, so that the soil fertility is increased, the drought resistance is increased, and the taste of crops is improved; the humic acid is contained, so that the development of root systems is promoted, and the absorption of fertilizers is promoted; contains bioactive silicon, increases leaf thickness, and can increase synthesis efficiency of chlorophyll; the fertilizer is rich in bacillus mucilaginosus, decomposes the solidified potassium element in the soil, improves the utilization rate of the fertilizer and activates soil nutrients; provides calcium element, and has effects of preventing and treating various physiological diseases caused by plant calcium deficiency.

The process adopts a three-layer granulation and coating process, and has the following effects:

firstly, the slow controlled release function is realized, so that the nutrition release is hierarchical, the release of nutrients in the inner layer is slowed down, the long-acting effect is realized, the fertilizer effect period is longer and is as long as 90 days.

Secondly, the culture protection layer is arranged, so that the culture is more stable.

And sufficient bacillus is added, so that customization is faster, and the effect of preventing soil-borne diseases is better.

Thirdly, the strain has a protective layer, can resist the key of the bactericide and can be mixed with the low-toxicity bactericide for use.

Nutritive elements and plant stimulin are added to reach the aim of precocity without premature senility.

Example 2: microbial granular inoculant with biocontrol nutrition function

The hierarchical structure is formed mainly by three layers of granulation processes, including a grain core layer, a middle cladding layer and an outer cladding layer; compared with the embodiment 1, the preparation method mainly comprises the following steps of sequentially carrying out granulation and coating processes from the core layer, wherein the specific components of each layer are as follows:

1. granule core layer

The pellet core layer comprises the following raw materials in parts by weight:

45 parts of mineral fulvic acid with the humic acid content of 70 percent, 55 parts of biochemical fulvic acid with the organic matter content of 72 percent, 6 parts of amino acid liquid, 7 parts of lactobacillus plantarum fermentation stock solution and 0.5 part of bacillus subtilis fermentation liquor,

2. middle cladding

10 parts by weight of the whole granule, and the raw materials of the middle coating layer comprise the following components in parts by weight:

contains 38.8 percent of free amino acid, 45 percent of enzymolysis feather powder, 39 percent of protein, 18 percent of soybean meal, 6 percent of 100 hundred million/g bacillus mucilaginosus, 3 percent of 1000 hundred million/g bacillus subtilis and 0.03 percent of 2- (3, 4-dichlorophenoxy) triethylamine synergist

3. Outer cladding

Accounting for 20% by weight of the whole pellet.

The raw material of the outer cladding is obtained by the following method: adding organic silicon, wherein the ratio of citric-soluble silicon to water-soluble silicon is 8:2, adding active silicon into 0.13% citric acid aqueous solution according to the mass ratio of 1:2, and uniformly mixing; adding 3% of starch, 0.5% of polyaspartic acid and 2% of calcium carbonate into the mixture, and stirring to obtain a suspension to obtain the outer-layer guniting ingredient.

The humic acid content is as high as 39.6%, the growth of crops is effectively promoted, and the rooting of the crops is stimulated.

Example 3: microbial granular inoculant with biocontrol nutrition function

1. granule core layer

The pellet core layer is 58% of the whole pellet by weight, and the raw materials of the pellet core layer comprise the following components in parts by weight:

45 parts of mineral fulvic acid with humic acid content of 62%, 55 parts of biochemical fulvic acid with organic matter content of 75%, 23 parts of amino acid liquid, 12 parts of lactobacillus plantarum fermentation stock solution and 0.8 part of bacillus subtilis fermentation liquor,

2. middle cladding

70 parts of enzymolysis feather powder containing 42 percent of free amino acid, 25 parts of soybean powder containing 35 percent of protein, 18 parts of bacillus mucilaginosus of 100 hundred million/g, 5 parts of bacillus subtilis of 1000 hundred million/g and 0.125 part of 2- (3, 4-dichlorophenoxy) triethylamine synergist.

3. Outer cladding

Accounting for 22% by weight of the whole pellet.

The raw material of the outer cladding is obtained by the following method: adding organic silicon, wherein the ratio of citric-soluble silicon to water-soluble silicon is 9.5:0.8, adding active silicon into 0.15% citric acid aqueous solution according to the mass ratio of 1:2.5, and uniformly mixing; adding 5% of starch, 1% of polyaspartic acid and 4.8% of calcium carbonate into the mixture, and stirring to obtain a suspension to obtain the outer-layer guniting ingredient.

The fertilizer has the advantages of sufficient nitrogen element, high silicon and calcium content, contribution to crop growth, thickening and greening of leaves, improvement of photosynthesis efficiency and accumulation of more nutrients in a limited time.

Example 4: application of the microbial inoculum of example 1 to celery test

1. Brief introduction to crop

Celery, belonging to the family of Umbelliferae. There are two kinds of water fennel and celery, which have similar functions, and the medicinal one is preferably celery.

Celery not only has rich nutrition, but also has medicinal value. Celery is rich in nutrients such as vitamin A, vitamin B, vitamin C, carotene, thiamine, riboflavin, folic acid, dietary fiber, nicotinic acid and the like, and trace elements such as calcium, ferrum, zinc, selenium, copper and manganese. Celery is cool in nature, deficient and cold in spleen and stomach and has good conditioning effect on hypertension, headache, dizziness, feverish sensation and polydipsia, jaundice, edema, difficult urination and heat, irregular menstruation and other diseases. It contains a large amount of colloidal calcium carbonate, is easily absorbed by human body, can supplement calcium required by legs, and can prevent lower half body edema. Meanwhile, the health-care food is an ideal green weight-losing food.

The celery seeds are small, the lowest germination temperature is 4 ℃, the optimal germination temperature is 15-20 ℃, the seedling top soil strength is weak, and the seedling emergence is slow. The seeds are sowed in the plots with high temperature, hardened soil and water accumulation in the ridges, the seedlings are not favorable to emerge, and the seeds are easy to germinate under the bright condition. Celery seedlings grow slowly, are long in seedling period and are easily damaged by weeds, and management needs to be enhanced in seedling culture. Celery likes cold, cool and humid climate, and belongs to semi-cold-resistant vegetables; it does not resist high temperature. Drying can resist short-term low temperature below zero.

2. Knowledge of crop physiology

The main reason for root rot of the dead celery seedlings is continuous cropping, so that certain nutrient components in soil are lacked, the growth of celery seedlings is influenced, harmful bacteria exist in the soil, and the celery seedlings continue to spread; the unfermented farmyard manure is applied, thus facilitating the transmission of germs and worm eggs and seriously harming the growth and development of celery.

Continuous cropping refers to the phenomenon that after the same crop or nearby crops are continuously planted, the growth condition is poor, the yield is reduced, the quality is reduced, and the diseases and insect pests are serious even under normal management.

The following hazards are mainly found:

2.1 deterioration of physicochemical Properties of soil and Secondary salinization

The soil is planted in successive years, the granular structure of the soil is destroyed, the soil is hardened, and the permeability is deteriorated; the large amount of fertilizer is applied, so that salt in the soil is excessive, secondary salinization and acidification of the soil are generated, the effectiveness of soil nutrients is influenced, and physiological diseases are caused.

2.2 imbalance of soil nutrient

As the absorption selectivity of the same crop to nutrient elements is consistent, the fertilization is relatively fixed in the planting process, imbalance of soil nutrients is caused, the proportion of nutrients in the crop is disordered, nutrient deficiency symptoms are easy to appear, and finally, the growth is hindered, and the yield and the quality are reduced.

2.3 autotoxicity of crops

The metabolite of pathogenic microorganism and the secretion of plant residue and root system can inhibit the growth and development of the same or similar crops planted in the same or next crop.

2.4 destruction of the ecological Environment of the soil

The change of the physical and chemical properties of soil and the specific root secretion of crops determine the variety of rhizosphere microorganisms, the growth and the propagation of beneficial microorganisms such as azotobacter, photosynthetic bacteria, actinomycetes and the like are inhibited by long-term continuous cropping, and harmful microorganisms are propagated in large quantity, so that pathogenic bacteria are continuously accumulated in the past, the soil-borne diseases are serious, and the growth and the development of the crops are restricted.

3. Basic information of grower

3.1, site: the temple in the Binzhou city of Shandong province is town in village.

3.2 planting time: number 4 month 8 of 2019

3.3 planting conditions: celery is continuously planted for three years, the variety is Jinnan celery No. 1, the length of the greenhouse is 60m, the width of the greenhouse is 8m, a single area is planted in different areas with the square of 20, direct seeding is carried out in a No. 8 planting mode in No. 4 month in 2019, and 25Kg of diammonium phosphate and 10Kg of urea are used as base fertilizers.

3.4 data at the beginning of the experiment: selecting two land blocks with equivalent growth vigor in the same greenhouse for treatment in 19 days 4 months 4 in 2019, wherein the seedlings are 4-5cm high at the 7 th day of emergence, and the dead seedlings appear, weak seedlings, thin and yellow stalks.

4. Design of experiments

Two ridges in the same greenhouse are taken for growing, wherein the control group uses a commercial continuous cropping resistant medicament, the experimental group uses a civil engineering package starting treatment, the interval is 12 days, and the result is observed in No. 5/1 in 2019.

4.1 control group: the organic fertilizer sold in the market is applied on 500kg of base fertilizer per mu, and 50kg of macroelement water-soluble fertilizer

4.2 Experimental group: the granular bacterial agent of white and black is applied on 500Kg base per mu, and 50Kg of macroelement water soluble fertilizer.

Macroelement water-soluble fertilizer: N-P2O5-K2O ═ 20-10-10.

5. Results of the experiment

TABLE 1 Experimental results after 12 days of treatment

6. Conclusion of the experiment

Through the experiments, the damage of continuous cropping to crops is quite large, the economic loss is also huge, and the control is carried out fundamentally, the root system culture is emphasized in the seedling stage, and the root system is reserved for the rapid growth in the later stage. The comparison shows that the length of the experimental group is obviously better than that of the control group, and the experimental group is 34.9 percent, 24.1 percent and 6.25 percent higher than that of the control group in terms of plant height, effective leaf area and stem leaf number, and has obvious advantages; the experimental group is higher than the control group by 52.3 percent in the aspect of promoting the development of the celery root system, and the advantages are obvious; the green of the continuous cropping dead seedlings is reduced by 13.8 percent. In conclusion, the effect of the civil engineering package is remarkable in the aspect of preventing the continuous cropping diseases of crops.

Claims

1. A microbial granular microbial inoculum with biocontrol nutrition function is characterized by comprising 55-75 parts of a granular core layer, 5-35 parts of a middle cladding layer and 5-25 parts of an outer cladding layer which are sequentially coated from inside to outside in parts by weight,

the raw material of the granular core layer comprises a product obtained by performing composite fermentation on an organic fermentation substrate by lactobacillus plantarum and bacillus subtilis;

2. The microbial granular inoculum with the biocontrol nutrition function as in claim 1 is characterized in that the raw materials of the granular core layer comprise, by weight:

3. The microbial granular inoculum with the biocontrol nutrition function as in claim 1, wherein the processing technology of the granular layer comprises the following steps:

fermenting under natural conditions, stirring for 1min after 32-36 hr in the fermentation process at a rotation speed of 80 rpm, stirring for 1min every 6-8 hr, and fermenting for 5-10 days to obtain granule core layer ingredient;

carrying out ammoniation granulation on the ingredients of the particle core layer, drying at the high temperature of 220-280 ℃, and carrying out sieving treatment, wherein the diameter of the screened particles is between 2 and 3.5mm, and the moisture is less than or equal to 25 percent, thus obtaining the particles of the particle core layer.

4. The microbial agent with the biocontrol nutrition function as claimed in claim 1, wherein the raw materials of the middle coating layer comprise, by weight: 45-75 parts of enzymolysis feather powder containing more than or equal to 40 percent of free amino acid, 15-25 parts of soybean powder containing more than or equal to 30 percent of protein, 5-10 parts of 100 hundred million/g of bacillus mucilaginosus, 3-5 parts of 1000 hundred million/g of bacillus subtilis and 0.025-0.125 part of 2- (3, 4-dichlorophenoxy) triethylamine.

5. The microbial granular inoculum with the biocontrol nutrition function of claim 1, wherein the processing technology of the middle cladding layer comprises the following steps:

6. The microbial inoculum of claim 1, which is prepared from the raw materials of the outer coating layer by the following method: uniformly mixing active silicon and 0.1-0.15% citric acid aqueous solution according to the mass ratio of 1: 2-2.5; adding starch 3-5% of the total weight of the outer cladding, polyaspartic acid 0.5-1% of the total weight of the outer cladding, and calcium carbonate 2-5% of the total weight of the outer cladding, and stirring to obtain suspension to obtain the outer layer guniting ingredient.

7. The microbial granular inoculum with the biocontrol nutrition function of claim 1, wherein the active silicon comprises citric-soluble silicon and water-soluble silicon in a ratio of 8: 2-9.5: 0.5.

8. The microbial inoculum of claim 1, wherein the outer coating is processed by the following steps: and (3) using a guniting granulation method, uniformly attaching the three cladding ingredients to the surface of the middle cladding in a guniting mode, drying at the temperature of 80-110 ℃, and sieving to ensure that the particle diameter is between 3-5mm and the whole water content is less than 25%.

9. The microbial granular inoculum with the biocontrol nutrition function of claim 1, which is characterized in that the finished product of the microbial inoculum granules contains 40-60% of organic matters, 5-20% of proteins, 10-40% of humic acid and 0.2-15 hundred million/g of bacteria.