KR101164939B1 - The method of a microbial products using the Slurry Compsiting and BiofilterationSCB as a culture medium of Lactobacillus - Google Patents

Abstract

The present invention relates to a method for preparing microbial liquor containing lactic acid bacteria cultured using SCB liquor ratio (Slurry compositing and biofilteration liquor, hereinafter referred to as SCB liquor ratio) as a nutrient source of the medium, and more specifically, adding a carbon source to the SCB liquor ratio. The present invention relates to a method for producing a microbial preparation in which lactic acid bacteria are cultured by adding a carbon source and a nitrogen source to the SCB liquid ratio, and adding a carbon source, a nitrogen source and an inorganic substance to the SCB liquid ratio as a nutrient source of the medium.
Lactic acid bacteria (Lactobacillus acidophilus) produces the organic substances degrading enzyme (protease, cellulase, amylase and the like), vitamins (foric acid), amino acids (proline and the like), growth hormones, nucleic acids (uracil, adenine, and so on). When the lactic acid bacteria are activated, seed germination and root development are promoted, and organic acids (acetic acid, lactic acid, etc.) are produced, solubilizing phosphoric acid by chelate bonds with cations of insoluble phosphate compounds.
This lactobacillus microbial liquor contains lactic acid bacteria and fertilizer nutrients at the same time, which has the effect of increasing the number of leaves, leaves and live weight of the crop. In addition, the microbial solution liquid ratio according to the present invention is effective in utilizing as a sprinkler or drip tube without clogging when applied to the tube system because of the low suspended solids content.
In addition, the use of microbial fertilizers cultured lactic acid bacteria as in the present invention effectively decomposes and absorbs large organic matter, the taste of agricultural products is good, and there are a lot of antioxidants to prevent cancer, high value-added resources and environmental pollution of livestock manure Has a significant effect on prevention.

Description

The method of a microbial products using the Slurry Compsiting and Biofilteration (SCB) as a culture medium of Lactobacillus}

The present invention relates to a method for preparing microbial liquor containing lactic acid bacteria cultured using SCB liquor ratio (Slurry compositing and biofilteration liquor, hereinafter referred to as SCB liquor ratio) as a nutrient source of the medium, and more specifically, adding a carbon source to the SCB liquor ratio. The present invention relates to a method for producing a microbial preparation in which lactic acid bacteria are cultured by adding a carbon source and a nitrogen source to the SCB liquid ratio, and adding a carbon source, a nitrogen source and an inorganic substance to the SCB liquid ratio as a nutrient source of the medium.

To date, livestock manure has been used in compost or liquefaction in terms of livestock farms. When livestock raising is small, manure from livestock farms has been composted. However, as the number of breeding heads increases, the type of slurry is increased for human convenience. It became. The liquid fertilizer is a liquid fertilizer, but since a large amount of solids is mixed, it is used to spray agricultural land at the expense of crop cultivation.

When manure is composted by the SCB composting method, the SCB liquor leaching from the sawdust layer has little suspended solids (SS, Sespended solids) compared to the conventional liquor storage tank. It has a small, fast-acting inorganic nutrient ratio, and is liquefied in a stabilized state, so it can be used in the form of spraying or fertilization for plant cultivation.

When composting livestock manure, there is a loss of fertilizer components such as nitrogen, and there is a disadvantage in that complete fermentation effect cannot be expected when storage liquid is fertilized, but SCB liquid fertilizer does not have odor and gas damage to crops. Can be used for purposes. In addition, based on the physicochemical properties of SCB liquid ratio, it is highly applicable as a microbial production medium.

In recent years, the soil is gradually losing its buffering capacity due to severe environmental pollution caused by industrialization and excessive application of chemical fertilizers and pesticides. As a result, in the process of growing crops, the capacity for cultivation decreased significantly, and the resistance to pests deteriorated. In the case of plant cultivation, the accumulation of chemical fertilizers, etc., may cause poor soil chemistry and lower crop productivity.

In order to alleviate the environmental pollution problem which is serious due to the above problems, interest in the use of organic farming and refraining from the use of chemical fertilizers and chemical pesticides in the agricultural field is increasing. Organic fertilizers widely used in organic farming methods have been commonly made by mixing fermentation with sawdust, bark and agricultural by-products, which are commonly used as moisture control agents.

Since these methods depend only on natural fermentation, it is difficult to control the components of the fertilizer as desired, and there is also a problem that various microorganisms may be incorporated into the fertilizer and become a breeding ground for pathogenic microorganisms.

Lactic acid bacteria (Lactobacillus acidophilus) produces the organic substances degrading enzyme (protease, cellulase, amylase and the like), vitamins (foric acid), amino acids (proline and the like), growth hormones, nucleic acids (uracil, adenine, and so on). When the lactic acid bacteria are activated, seed germination and root development are promoted, and organic acids (acetic acid, lactic acid, etc.) are produced, solubilizing phosphoric acid by chelate bonds with cations of insoluble phosphate compounds.

When cultivating lactic acid bacteria using SCB liquid ratio as a nutrient source of lactic acid bacteria medium, the lactic acid bacteria inhibit the growth of harmful microorganisms and rapid decay of organic matters with strong bacteriostatic power of organic acids, and produce anti-pathogenic factors to enhance disease resistance to growing crops. It works. In addition, the lactic acid bacteria may be useful for crop cultivation in a poor environment by helping to quickly absorb the nutrients while at the same time facilitate the rooting of the crop even in a high temperature and high humidity environment.

However, SCB liquid ratio contains minerals, but it is difficult to cultivate lactic acid bacteria due to lack of energy source. In addition, there was a problem that the liquid fertilizer containing lactic acid bacteria is difficult to be used in organic farming because no alternative medium for lactic acid bacteria culture was developed. In addition, the composition of the agricultural lactic acid bacteria medium is expensive, the situation that mass production is a big cost. Therefore, there is a need for the development of alternative medium for agriculture, which is low in medium cost and balanced with the ingredients necessary for the growth of microorganisms.

The present invention is to solve the above problems, the present invention is to reduce the cost of culturing the lactic acid bacteria to facilitate the economical access to this, the conventionally utilized to maximize the effect of the organic method using lactic acid bacteria It provides a method for producing a microbial preparation containing lactic acid bacteria cultured by replacing the lactic acid bacteria culture medium, and using the replacement medium.

In addition, the present invention provides a replacement medium having an improved lactic acid bacteria culture effect compared to MRS medium mainly used as a medium for selection of lactic acid bacteria, and easy to use as a medium, low-cost and easy to purchase, lactic acid bacteria cultured using the replacement medium It provides a method for producing a microbial preparation containing.

The present invention is characterized by a method for producing a microbial preparation in which lactic acid bacteria are cultured in a medium in which a carbon source is added to the SCB liquid fertilizer.

In addition, the present invention is characterized by a method for producing a microbial preparation in which lactic acid bacteria are cultured in a medium in which a nitrogen source is further added to the medium.

In addition, the present invention is characterized by a method for producing a microbial preparation in which lactic acid bacteria are cultured in a medium in which minerals are further added to the medium.

In addition, the lactic acid bacteria of the present invention is characterized in that the Lactobacillus acidophilus ( Lactobacillus acidophilus ).

In addition, the carbon source of the present invention is characterized in that the molasses 0.5 ~ 5 w / v% with respect to 100 ml SCB liquid ratio.

In addition, the nitrogen source of the present invention is characterized in that the animal amino acid of 0.5 to 5 w / v% with respect to 100 ml of SCB liquid ratio.

In addition, the mineral of the present invention is characterized in that the seaweed powder 0.1 ~ 0.5w / v% with respect to 100ml SCB liquid ratio.

In addition, the mineral of the present invention is characterized in that sodium acetate (sodium acetate) 0.5 ~ 1 w / v% and dipotassium phosphate (0.1 ~ 0.3 w / v%) with respect to 100 ml SCB liquid ratio.

The present invention has a remarkable effect in reducing crop production costs by using SCB liquor as a medium representing the number of microbial bacteria equivalent to that of expensive lactic acid bacteria selection medium, and clogging the fertilization system by using liquor cultured with lactic acid bacteria using SCB liquor as a medium. It can be used as a sprinkler or drip tube without this.

In conventional chemical farming and traditional organic farming, the crop absorbs only small amounts of inorganic nutrients, whereas the microbial fluid ratio in which lactic acid bacteria are cultured as in the present invention is a large organic molecule of amino acids (nitrogen), nucleic acids, carotene, vitamins, Bioactive substances such as hormones and sugars are decomposed and absorbed, which is effective for promoting crop growth, enhancing the vitality of soil microorganisms, and having a significant effect on suppressing harmful microorganisms.

In addition, the present invention has a remarkable effect in increasing the effect of the organic method using the lactic acid bacteria, including a high density of bacteria (nutrition cells) similar to the MRS medium used as lactic acid bacteria selection medium.

Recently, the use of microbial fertilizers has been increased due to the need for eco-friendly organic methods, and the present invention is a method for producing a microbial preparation containing lactic acid bacteria cultured using the SCB liquid ratio as a medium.

Using microbial liquid fertilizers using SCB liquid fertilizers, simultaneous effects of microorganisms and fertilizers are possible. When the microbial fluid ratio production system and crop production system are established, it is very useful as a useful agricultural material.

In Korea, the demand for liquid fertilizer is increasing due to the development of eco-friendly agriculture, but the systematic research on the production of microbial liquid fertilizer containing useful microorganisms is insufficient, and liquid fertilizer products produced by private companies are struggling. It is being distributed without any price, and the price of the product is very high, which is causing farmers to increase their production cost.

Hereinafter, the present invention will be described in more detail with reference to the following examples, which are presented to aid the understanding of the present invention but are not intended to limit the present invention.

The present invention is a method for producing a microbial preparation containing lactic acid bacteria cultured by using the addition of an additive to the SCB liquid ratio as a medium, in which molasses, molasses and animal amino acids, molasses and animal amino acids, and minerals are added to the medium, respectively. .

The present invention uses the SCB liquid ratio as the lactic acid bacteria culture medium, the SCB liquid ratio refers to the dark liquid fertilizer in which the compost filtrate is lowered to the bottom of the compost by gravity in the overhydrated state (more than saturated water) of the compost.

The SCB microbial fluid ratio is effective in supplying nutrients to plants, promoting plant growth, improving soil structure and enhancing soil water retention, containing ingredients useful for crop growth, and having low suspended solids. It is possible to use as a sprinkler or drip tube because there is no clogging of the tube system.

The physicochemical characteristics of SCB liquid ratio are shown in Table 1. The SCB liquid ratio is low in phosphoric acid, calcium and magnesium and high in potassium. Suspended solid content of the concentrate ratio is very low (352.5mg / ℓ), so there is no blockage problem when cultivating the house tube, the nitrogen content of the concentrate ratio was 528.6mg / ℓ This has reached a possible level.

Physicochemical Properties of SCB Liquid Ratio Items TN
(mg / ℓ) NO ₃ -N
(mg / ℓ) NH ₄ -N
(mg / ℓ) P ₂ O ₅
(mg / ℓ) CaO
(mg / ℓ) MgO
(mg / ℓ) K ₂ O
(mg / ℓ) Suspended
solid
(mg / ℓ) Compost leachate 528.6 98 176.6 159.3 45.5 22.7 1,447.6 352.5

The composition of MRS medium, the selection medium of Lactobacillus acidophilus, is as follows. In the present invention, in consideration of the composition of the composition of the MRS medium, the replacement medium that can cultivate lactic acid bacteria by supplementing the components lacking in SCB liquid ratio with by-products was examined.

Composition of MRS Medium Casein peptone, tryptic digest 10.00 g Meat extract 10.00 g Yeast extract 5.00 g Glucose 20.00 g Tween 80 1.00 g K ₂ HPO ₄ 2.00 g Na-acetate 5.00 g (NH ₄ ) ₂ citrate 2.00 g MgSO ₄ × 7 H ₂ O 0.20 g MnSO ₄ × H ₂ O 0.05 g Distilled water 1000.00 ml pH 6.2 to 6.5

When using SCB liquid ratio as lactic acid bacteria nutrient source, use SCB liquid ratio after autoclaving for 15 minutes at 15 at 121 ℃. The culture temperature of lactic acid bacteria is a temperature at the time of normal lactic acid bacteria culture in the range of 30-45 degreeC, and can be added or decreased suitably as needed. As the lactic acid bacteria, various known ones may be used, and in the present invention, Lactobacillus acidophilus was used.

The addition of molasses is a carbon source for aiding the cultivation of lactic acid bacteria, and the present invention is not limited to molasses, and processed and fermented by-products or waste products such as concentrated aquatic products and foods can be used. Examples include rice bran, chaff, rice straw, corn husk, corn powder, corn leaves, corn stalks, soybean husks, bran, straw, sugar cane processing by-products, sugar cane, sawdust and wood processing by-products, waste paper, cassava, potatoes and sweet potato processing by-products. If it is carbon source, such as hay and blades of grass, it is possible to add.

The addition of animal amino acids is a nitrogen source for aiding the cultivation of lactic acid bacteria, and the present invention is not limited to animal amino acids, and the present invention can be added as long as it is a nitrogen source such as yeast, soybean meal, cottonseed meal, corn leachate, dairy by-products, and seafood processed dispersions.

The reason for adding sodium acetate and dipotassium phosphate as minerals is to be the same as that of MRS medium, which is an optimal medium for lactic acid bacteria, and the present invention is not limited thereto. And other conventional ammonia sulfate. Inorganic salts such as sodium nitrate, calcium carbonate, magnesium sulfate and phosphate, and trace elements such as Fe, Mn, Zn, Co, Mo, and B can be added.

When fertilizing and foliar fertilization of the liquid fertilizer prepared as described above contains lactic acid bacteria, mycelium is formed on the plant roots to facilitate the supply of nutrients, thereby promoting continuous differentiation and growth of roots, especially in high temperature and high humidity environments. In addition to facilitating rooting of plants, they can quickly absorb nutrients, which can be useful for growing crops in poor environments.

Experimental Example 1 Lactic Acid Bacteria Culture Using SCB Fluid Ratio as a Medium

This example is to investigate the composition of the SCB liquid ratio for the purpose of using the SCB liquid ratio as a medium for the production of Lactobacillus acidophilus (Lactobacillus acidophilus), and the Lactobacillus Escidophilus in additional nutrient medium such as SCB liquid ratio and molasses ( Lactobacillus acidophilus) density was examined.

As the SCB liquid fertilizer material of this embodiment, the SCB liquid fertilizer discharged from the SCB composting facilities of pig farms was used. When the SCB liquid ratio was used as a lactic acid bacteria nutrient source, the SCB liquid ratio was used after autoclaving for 15 minutes at 15 at 121 ° C.

Lactobacillus acidophilus 3.0 × 10 ⁶ cfu / ml Lactic acid bacteria culture medium and 200 ml filtrate were used as the main fermentation source and MRS broth medium was used as a control. After the culture of lactic acid bacteria at 35 ℃ culture conditions were compared. The inoculated strain was used as Lactobacillus acidophilus, and the inoculated strain was performed as follows.

Example 1 In the present invention, 100 ml of SCB solution was incubated with Latobacillus acidophilus.

Example 2 In the present invention, Lactobacillus acidophilus was cultured in a medium containing 2 g of molasses as a carbon source in 100 ml of SCB liquid ratio.

Example 3 In the present invention, Latobacillus acidophilus was cultured in a medium containing 2 ml of molasses as a carbon source and 2 g of an animal amino acid as a nitrogen source in 100 ml of SCB liquid ratio.

Example 4 Latobacillus acidophilus was cultured in a medium containing 2 g of molasses as a carbon source, 2 g of animal amino acid as a nitrogen source, and 0.2 g of seaweed powder in 100 ml of SCB liquid ratio.

Example 5 Lactobacillus ecidophilus (2 g of molasses as a carbon source, 2 g of animal amino acid, 0.5 g of sodium acetate and 0.2 g of dipotassium phosphate) was added to 100 ml of the SCB liquid ratio. Lactobacillus acidophilus ) was cultured.

Comparative Example 1 As a control, Lactobacillus acidophilus was cultured in MRS medium.

In the above example, the ratio of 100 ml of SCB liquid, 2 g of molasses as a carbon source, 2 g of animal amino acid and 2 g of seaweed powder or 0.5 g of sodium acetate and 0.2 g of dipotassium phosphate as a nitrogen source was matched with the composition ratio of the control MRS medium. For

Molasses 0.5 to 5 w / v%, animal amino acids 0.5 to 5 w / v%, seaweed powder 0.1 to 0.5 w / v%, sodium acetate 0.5 to 1 w / v%, depot Tesium phosphate may be appropriately added or decreased to a concentration of 0.1 ~ 0.3 w / v%, but is not limited to the above range, and includes a typical range necessary to achieve the object of the present invention at the level of those skilled in the art.

Lactobacillus acidophilus density in SCB fluid ratio medium Treatment Incubation time (0 hour) Incubation time (24 hours) Incubation time (48 hours) SCB 10 ⁵ x 5.4 0 0 SCB + Molasses 10 ⁵ x 3.0 10 ⁵ x 4.4 10 ⁶ x 1.1 SCB + molasses + amino acid 10 ⁵ x 1.0 10 ⁶ x 4.1 10 ⁶ x 9.4 SCB + molasses + amino acid + seaweed powder 10 ⁵ x 1.0 10 ⁷ x 1.1 10 ⁷ x 1.4 SCB + molasses + amino acids + minerals * 10 ⁵ x 2.0 10 ⁷ x 1.1 10 ⁷ x 9.5 MRS Badge (Control) 10 ⁵ x 4.0 10 ⁷ x 2.1 10 ⁷ x 2.3

Sodium acetate (0.5 g) + Dipotassium phosphate (0.2 g)

The results of the culture test of the lactic acid bacteria are shown in Table 2. The inoculation density was 10 ⁵ × 5.4 cfu / g when using the SCB fluid ratio, but most of them were killed after 24 hours of culture. The number of Lactobacillus acidophilus bacteria in culture medium supplemented with molasses, amino acids, and seaweed powder in the SCB fluid ratio was 10 ⁷ × 1.4 cfu / g, which was similar to that in cultured MRS medium. It was.

In addition, Lactobacillus acidophilus bacteria in culture medium supplemented with molasses, amino acids, and minerals (Sodium acetate + Dipotassium phosphate) in the SCB fluid ratio showed 10 ⁷ × 9.5 cfu / g, which was cultured for 48 hours. It can be seen that the number of bacteria similar to that of the control MRS medium, which is similar to that of the MRS medium of the lactic acid bacteria selection medium is cultured by the addition of by-products as the SCB liquid ratio.

Experimental Example 2 Effect of Application of Microbial Fertilizer Products on Plant Growth

In this experiment, the starting material was blue tooth lettuce and the test fertilizer was a microbial fertilizer prepared from a mixture of molasses, animal amino acids, and minerals (Sodium acetate and Dipotassium phosphate) in the medium of SCB liquid. The purpose of this study was to investigate the effect of fertilizer application on plant growth. The test area per treatment zone was 12m ² (3m × 4m), and the test zones were randomized and 3 repeated batches. Each test and control was treated as follows.

  Example 7 The test zone was treated with 20 L / 10a of microbial agent for composting and NPK treatment.

  Example 8 The test zone was treated with 40 L / 10a of microbial agent for composting and NPK treatment.

  Example 9 The test strips were treated with 80 L / 10a of microbial agent for composting and NPK treatment.

<Comparative Example 2> The control (conventional) was treated with compost and NPK.

The items to be investigated were leaf, leaf width, number of leaves, live weight, leaf weight, dry weight (70 ℃, 96 hours), quantity and leaf color (SPAD 502). The test material was Lactobacillus acidophilus 1.2 × 10 ^{It was 7} cfu / ml.

The planting distance was 20cm × 20㎝ and the fertilization method was fertilized three times with microbial agents at weekly intervals from 25 days after lettuce was settled and irrigated so that topsoil was sufficiently wetted during application. At the test site 1, the first investigation was conducted after 40 days, and the second investigation was performed after 57 days. At the test site 2, the first investigation was conducted after 39 days, and the second investigation was performed after 56 days.

end. Experimental results at test site 1

 Effect of Disclosed Microbial Fertilizers on the Growth of Lettuce (Primary Survey, 40 After Formulation) process ground game
(recast) Leaf
(cm) Leaf width
(cm) Leaf color
(SPAD 502) Control  16.3 a * 16.7 a 12.8 a 31.5 a Test strip 17.7 a 18.8 a 13.6 a 33.9 a Test strip 18.3 a 19.2 a 14.3 a 35.0 b Test strip 19.3 a 18.4 a 14.5 a 35.1 b

* DMRT at 5% level

 Effect of Disclosed Microbial Fertilizers on the Growth of Lettuce (Secondary Survey, Post Meal 57) process ground game
(recast) Leaf
(cm) Leaf width
(cm) Leaf color
(SPAD 502) Control 29.3 b * 17.4 a 12.2 a 32.7 a Test strip 29.0 b 19.3 a 13.6 a 32.0 a Test strip 31.3 a 20.3 a 13.3 a 33.3 a Test strip 31.7 a 21.2 a 13.9 a 33.7 a

* DMRT at 5% level

Lettuce Individual Weight process Leaves (g / piece) Live weight (g / week) Among buildings (g / week) Primary Secondary Primary Secondary Primary Secondary Control 4.6 a * 3.0 a 79.6 a 94.6 a 8.7 a 10.0 a Test strip 4.7 a 3.1 a 82.0 a 96.8 a 9.3 a 11.7 a Test strip 4.8 a 3.5 a 85.4 b 105.4 b 11.3 a 12.7 a Test strip 4.9 a 3.9 a 89.8 b 106.4 b 11.9 a 12.7 a

* DMRT at 5% level

Quantity process Primary quantity
(kg / 10a) 2nd quantity
(kg / 10a) Total quantity
(kg / 10a) Quantity index Control 1,751.2 a * 2,015.2 a 3,766.4 a 100 Test strip 1,804.0 a 2,085.6 a 3,889.6 a 103 Test strip 1,898.8 a 2,230.8 a 4,129.6 a 110 Test strip 1,975.0 a 2,274.8 a 4,249.8 a 112

* DMRT at 5% level

I. Experimental results at test site 2

  Effect of Disclosed Microbial Fertilizers on the Growth of Lettuce (Phase 1, Post-39) Treatment ground game
(recast) Leaf
(cm) Leaf width
(cm) Leaf color
(SPAD 502) Control 16.3 a * 19.1 a 13.8 a 32.2 a Test strip 17.0 a 19.3 a 14.6 a 36.0 a Test strip 16.7 a 19.9 a 15.3 a 33.7 a Test strip 17.7 a 22.0 a 15.4 a 30.8 a

* DMRT at 5% level

 Effect of Disclosed Microbial Fertilizers on the Growth of Lettuce (Secondary Survey, Post Meal 56) Treatment ground game
(recast) Leaf
(cm) Leaf width
(cm) Leaf color
(SPAD 502) Control 22.7 b * 20.4 b 12.3 a 32.9 a Test strip 25.3 ab 20.8 ab 12.0 a 33.7 a Test strip 27.7 a 22.2 a 12.6 a 30.9 a Test strip 28.3 a 22.7 a 13.3 a 29.2 a

* DMRT at 5% level

 Lettuce Individual Weight process Leaves (g / piece) Live weight (g / week) Among buildings (g / week) Primary Secondary Primary Secondary Primary Secondary Control 4.7 a * 3.4 a 76.5 a 81.8 a 9.3 a 10.7 a Test strip 4.7 a 3.4 a 78.8 a 86.2 a 10.3 a 12.0 a Test strip 4.8 a 3.2 a 80.7 a 87.7 a 11.3 a 12.0 a Test strip 4.9 a 3.2 a 84.5 a 89.9 a 11.3 a 11.7 a

* DMRT at 5% level

 Quantity process Primary quantity
(kg / 10a) 2nd quantity
(kg / 10a) Total quantity
(kg / 10a) Quantity index Control 1,683.0 a * 1800.3 a 3,483.3 a 100 Test strip 1,734.3 a 1,895.7 a 3,630.0 a 104 Test strip 1,774.7 a 1,928.7 a 3,703.4 a 106 Test strip 1,859.0 a 1,976.3 a 3,835.3 a 110

* DMRT at 5% level

Experimental Example 2 was carried out at test site 1, test site 2 to examine the effect on the growth and yield of the microbial fertilizers on lettuce. The treatment content was a control (traditional), a standard half dose application (test), a standard titration application (test), and a standard dose application (test). The test results obtained are summarized below.

In the growth characteristics of lettuce, leaf, leaf, and leaf width were increased in the standard microbial fertilizer, semi-quantitative fertilizer, and quantity and batch treatment compared to the control group. Significance of Duncan's multiple test results in all treatments at test site 1 and test site 2, except for the 1st and 2nd investigations, and the 2nd and 2nd trials. Did not appear.

Chlorophyll measurement using SPAD 502 showed no significant difference in the test place 1 and the test site 2 in the control group, titration and batch of microbial fertilizer.

The weight of lettuce showed no significant difference in leaf and dry weight in all treatments. However, the fresh weight of the lettuce increased in all treatments of microbial fertilizer baseline, appropriate amount and volume compared to the control. However, Duncan's multiple test result showed no significant difference with the control.

The yield of lettuce was increased by 3 ~ 12% (test site 1) and 4 ~ 10% (test site 2) in the control group. As a result of statistical processing of the yield, no significant significance was found at the 5% significance level in all treatments at Test Site 1 and Site 2.

As a result, no damage of lettuce was found in the test of microbial fertilizer application, and the increase of the number of leaves, leaves, and biomass was also shown in the control group (traditional) in the standard control, proper and batch treatments. It was. The yield of lettuce was increased by about 10% compared to the control in all the treated microbial fertilizers.

Claims (8)

As a method for producing a microbial preparation containing lactic acid bacteria cultured using the SCB liquid ratio as a medium,
Carbon source, nitrogen source, minerals are added to the SCB liquid ratio,
The carbon source is 0.5 to 5 w / v% molasses per 100 ml SCB liquid ratio,
The nitrogen source is 0.5 to 5 w / v% of animal amino acids per 100 ml SCB liquid ratio,
The mineral is contained 0.1 ~ 0.5w / v% seaweed powder with respect to 100ml SCB liquid ratio,
The SCB liquid ratio is a method for producing a microbial preparation containing lactic acid bacteria, characterized in that used for 15 minutes under pressure sterilization at 121 ℃ conditions. delete delete The method of claim 1,
The lactic acid bacterium is a method for producing a microbial preparation containing lactic acid bacteria cultured using the SCB liquid ratio, characterized in that Lactobacillus acidophilus ( Lactobacillus acidophilus ) as a medium. delete delete delete As a method for producing a microbial preparation containing lactic acid bacteria cultured using the SCB liquid ratio as a medium,
Carbon source, nitrogen source, minerals are added to the SCB liquid ratio,
The carbon source is 0.5 to 5 w / v% molasses per 100 ml SCB liquid ratio,
The nitrogen source is 0.5 to 5 w / v% of animal amino acids per 100 ml SCB liquid ratio,
The mineral is a microbial preparation, characterized in that it comprises 0.5 ~ 1 w / v% of sodium acetate and 0.1 ~ 0.3 w / v% of dipotassium phosphate per 100 ml SCB liquid ratio.