KR102574958B1 - Manufacturing method of microbial metabolite fertilizer using waste culture solution discarded after culturing microorganisms - Google Patents

Abstract

The present invention can significantly reduce the cost, manpower, and time for the treatment of waste culture fluid (microbial metabolites) by using waste culture fluid discarded after microbial culture as a raw material for fertilizer, and by utilizing the components contained in the metabolites, Microbial metabolite fertilizer using waste culture fluid discarded after culturing microorganisms that can provide growth promotion effect, improvement of soil fertility and decomposition ability of organic matter, improvement of nutrient absorption rate and enhancement of antioxidant effect. Discloses the manufacturing method of.

Description

Manufacturing method of microbial metabolite fertilizer using waste culture solution discarded after culturing microorganisms}

In the present invention, a first microorganism is inoculated into a medium, then cultured in a large amount in an incubator, the culture medium in the incubator is separated into a first microorganism and a first metabolite by centrifugation in a centrifuge, and then the first metabolite is recovered. forming a first macro element mixture by mixing with one or more first macro elements selected from the group consisting of nitrogen (N), phosphoric acid (P), and potassium (K); After inoculating the second microorganism in the medium, it is cultured in a large amount in an incubator, and the culture medium in the incubator is centrifuged in a centrifuge to separate the second microorganism and the second metabolite, and then the second metabolite is recovered and calcium ( forming a second major element mixture by mixing with one or more second major elements selected from the group consisting of Ca), magnesium (Mg), and sulfur (S); After inoculating the medium with a third microorganism, it is cultured in a large amount in an incubator, the culture medium in the incubator is centrifuged in a centrifuge to separate the third microorganism and a third metabolite, and then the third metabolite is recovered and zinc ( A mixture of trace elements by mixing one or more trace elements selected from the group consisting of Zn), iron (Fe), boron (B), copper (Cu), manganese (Mn), silicic acid, and molybdenum (Mo) forming a; Forming a microbial metabolite fertilizer by mixing the first macro-element mixture, the second macro-element mixture, and the trace element mixture; microbial metabolites utilizing waste culture fluid discarded after culturing microorganisms comprising: It relates to a method for producing fertilizer.

Recently, as environmental problems are directly related to the survival of mankind, especially the food problem, attention has been focused around the world. The development of modern agricultural technology has contributed greatly to the stable supply of food by increasing agricultural productivity, but has caused a new problem of environmental pollution. made it Soil pollution (fertilizer accumulation, etc.), water pollution (fertilizer leaching, etc.) and air pollution (fertilizer denitrification, etc.) due to the excessive use and input of artificial chemical fertilizers are intensifying pollution in rural areas. It not only directly and indirectly threatens health, but also damages the natural landscape and reduces the safety of crops. Accordingly, in order to minimize the environmental impact, a technology for establishing a sustainable resource recycling agricultural system is required.

On the other hand, microbial metabolites are also called postbiotics, and despite their excellent usability, they are classified as waste when not recycled, resulting in water treatment costs. In particular, since the metabolites of microorganisms are directly collected and treated by a licensed treatment company, the procedure is complicated and enormous costs are consumed for treatment. Therefore, in the field of agricultural technology, there is a need for a method that can usefully use microbial metabolites with excellent functionality for resource circulation and environmental preservation, but there is a lack of specific methods that can satisfy these needs.

The present invention is to solve the above problems, by using the waste culture solution discarded after microbial culture as a raw material of fertilizer, it is possible to significantly reduce the cost, manpower, and time for the treatment of the waste culture solution, and is excellent in crop growth. The purpose is to provide an eco-friendly functional fertilizer that can provide effects.

For the above purpose, the present invention inoculates a first microorganism in a medium and then cultures it in a large amount in an incubator, centrifugates the culture medium in the incubator in a centrifuge to separate the first microorganism and the first metabolite, and then recovering the first metabolite and mixing it with one or more first macroelements selected from the group consisting of nitrogen (N), phosphoric acid (P), and potassium (K) to form a first macroelement mixture; After inoculating the second microorganism in the medium, it is cultured in a large amount in an incubator, and the culture medium in the incubator is centrifuged in a centrifuge to separate the second microorganism and the second metabolite, and then the second metabolite is recovered and calcium ( forming a second major element mixture by mixing with one or more second major elements selected from the group consisting of Ca), magnesium (Mg), and sulfur (S); After inoculating the medium with a third microorganism, it is cultured in a large amount in an incubator, the culture medium in the incubator is centrifuged in a centrifuge to separate the third microorganism and a third metabolite, and then the third metabolite is recovered and zinc ( A mixture of trace elements by mixing one or more trace elements selected from the group consisting of Zn), iron (Fe), boron (B), copper (Cu), manganese (Mn), silicic acid, and molybdenum (Mo) Forming a; mixing the first major element mixture, the second macro element mixture, and the trace element mixture to form a microbial metabolite fertilizer, wherein the first microorganism is of the genus Agrobacterium sp. , alkali Genes defragrens ( Alcaligenes defragrans ), Alcaligenes piechaudii , Ampelomyces quisqualis ( Ampelomyces quisqualis ), Arthrobacter protophormiae , Arthrobotris oligospora ( Arthrobotrys oligospora ), Azospirillum brasilense ( Azospirillum brasilense ), Azospirillum lipoferum ( Azospirillum lipoferum ), Azotobacter chroococcum ( Azotobacter chroococcum ), Azotobacter vinelandii ( Azotobacter vinelandii ), Brady rhizobium Ponycum ( Bradyrhizobium japonicum ), Brevibacillus brevis ( Brevibacillus brevis ), Brevibacillus formosus ( Brevibacillus formosus ), Brevibacterium ammoniagenes ( Brevibacterium ammoniagenes ), Brevibacterium flavum ( Brevibacterium flavum ), brevi Bacterium linens, Brevibacterium otitidis, Brevibacterium otitidis, Burkholderia cepacia, Burkholderia pyrrocinia , Cellulomonas fimi ), Cellulomonas turbata , Delftia acidovorans, Dietzia natronolimnaea , Frateuria aurantia , Glomus intradishes ( Glomus intraradices ), Helicosporium nizamabadense , Klebsiella mobilis , Lysinibacillus boronitolerans, Lysinibacillus fusiformis, Lysinibacillus fusiformis Sobacter antibiotic cus ( Lysobacter antibioticus ), Microbacterium aurum ( Microbacterium aurum ), Nocardiopsis dasonvillei ( Nocardiopsis dassonvillei ), Pessylomyces fumosoroseus ( Paecilomyces fumosoroseus ), Paecilomyces Japonica ( Paecilomyces japonica ), Paecilomyces lilacinus , Paenibacillus amylolyticus , Paenibacillus azoreducens , Paenibacillus chibensis , Panibacillus ren Timobus ( Paenibacillus lentimorbus ), Panibacillus macerans ( Paenibacillus macerans ), Panibacillus polymyxa ( Paenibacillus polymyxa ), Pantoea genus. S32 ( Pantoea sp. S32 ), Pediococcus Serevisiae ( Pediococcus cerevisiae ), Pediococcus halophilus ( Pediococcus halophilus ), Pedobactor ginseng ginsengisoli ( Pedobacter ginsengisoli ), Photorhabdus luminescens ( Photorhabdus luminescens ) . Sys ( Pseudomonas _ _ panipatensis ), Pseudomonas putida , Pseudomonas striata, Pseudomonas striata, Rhizopus delma , Rhizopus japonicus, Rhizopus oryzae, Tetrathiobacter kash One selected from the group consisting of Tetrathiobacter kashmirensis , Trichoderma hamatum , Trichoderma harzianum , and Variovorax boronicumulans , wherein the second microorganism is Bacillus amil Bacillus amyloliquefaciens , Bacillus brevis , Bacillus cereus , Bacillus circulans , Bacillus edaphicus , Bacillus glucanolyticus ), Bacillus licheniformis , Bacillus macerans , Bacillus mesonae , Bacillus mojavensis, Bacillus mucilaginosus , Bacillus natto ( Bacillus natto ), Bacillus polymyxa ( Bacillus polymyxa ), Bacillus pumilus ( Bacillus pumilus ), Bacillus subtilus ( Bacillus subtilis ), Bacillus bilis mortis ( Bacillus vallismortis ), Bacillus velezensis ( Bacillus velezensis ), Bacillus Megaterium ( Bacllius megaterium ), Bacillus macroides ( Bcillus macroides ), Streptomyces asoensis ( Streptomyces asoensis ), Streptomyces carpinensis ( Streptomyces carpinensis ), Streptomyces fradiae ( Streptomyces fradiae ), Streptomyces Seth griseo chromogejinseu ( Streptomyces griseochromogenes ), Streptomyces griseus ( Streptomyces griseus ), Streptomyces halstedii ( Streptomyces halstedii ), Streptomyces niger ( Streptomyces niger ), Streptomyces violaseus niger ( Streptomyces violaceusniger ), Rhodobacter azotoformans ( Rhodobacter azotoformans ), Rhodobacter capsulata ( Rhodobacter capsulata ), Rhodobacter capsulatus ( Rhodobacter capsulatus ), Rhodobacter rubrum ( Rhodobacter rubrum ), Rhodobacter spheroidis ( Rhodobacter sphaeroides ), Rhodopseudomonas capusulata ( Rhodopseudomonas capusulata ), Rhodopseudomonas palustris ( Rhodopseudomonas palustris ), Rhodopseudomonas Spheroides ( Rhodopseudomonas sphaeroides ), Rhodopseudomonas viridis ( Rhodopseudomonas viridis ), Saccharomyces amensis ( Saccharomyces anamensis ), Saccharomyces carlsbergensis ), Saccharomyces cerevisiae , Saccharomyces sake ( Saccharomyces sake ), Pichia anomala ( Pichia anomala ) and Pichia desticola ( Pichia deserticola ) and Pichia stipitis ( Pichia stipitis ), and the third microorganism is Acetobacter diazotrophicus ( Acetobacter diazotrophicus ), Acetobacter peroxidans ( Acetobacter peroxydans ), Acinetobacter Calcoaceticus ( Acinetobacter calcoaceticus ), Lactobacillus acidophilus ( Lactobacillus acidophilus ), Lactobacillus bulgaricus ( Lactobacillus bulgaricus ), Lactobacillus casei ( Lactobacillus casei ), Lactobacillus casei subsp . Lactobacillus confusa , Lactobacillus crispatus , Lactobacillus curvatus , Lactobacillus delbrueckii , Lactobacillus fermentum , Lactobacillus Helveticus, Lactobacillus paracasei, Lactobacillus perolens, Lactobacillus plantarum , Lactobacillus rhamnosus, Lactobacillus reuteri ), Lactobacillus salivarius , Lactococcus lactis, Stephanoascus ciferrii , Streptococcus cremoris , Streptococcus lactis , Streptococcus Streptococcus thermophilus , Aspergillus niger , Aspergillus oryzae , Candida kefir , Candida utilis and Candida valida ) It is characterized in that it is selected from the group consisting of.

In addition, the present invention inoculates a first microorganism in a medium, then cultures in a large amount in an incubator, centrifugates the culture medium in the incubator in a centrifuge to separate the first microorganism and the first metabolite, and then the first metabolite. forming a first macro element mixture by recovering and mixing with one or more first macro elements selected from the group consisting of nitrogen (N), phosphoric acid (P), and potassium (K); After inoculating the second microorganism in the medium, it is cultured in a large amount in an incubator, and the culture medium in the incubator is centrifuged in a centrifuge to separate the second microorganism and the second metabolite, and then the second metabolite is recovered and calcium ( forming a second major element mixture by mixing with one or more second major elements selected from the group consisting of Ca), magnesium (Mg), and sulfur (S); After inoculating the medium with a third microorganism, it is cultured in a large amount in an incubator, the culture medium in the incubator is centrifuged in a centrifuge to separate the third microorganism and a third metabolite, and then the third metabolite is recovered and zinc ( A mixture of trace elements by mixing one or more trace elements selected from the group consisting of Zn), iron (Fe), boron (B), copper (Cu), manganese (Mn), silicic acid, and molybdenum (Mo) forming a; mixing the recovered third metabolite and the second macroelement, adjusting the pH by adding an organic acid, and then performing a chelation reaction to form a third macroelement mixture; mixing the first major element mixture, the second macro element mixture, the third macro element mixture, and the trace element mixture to form a microbial metabolite fertilizer; wherein the first microorganism is of the genus Arrowbacterium ( Agrobacterium sp. ), Alcaligenes defragrans, Alcaligenes defragrans , Alcaligenes piechaudii, Ampelomyces quisqualis , Arthrobacter protophormiae , Arthrobotrys oligospora ( Arthrobotrys oligospora ), Azospirillum brasilense ( Azospirillum brasilense ), Azospirillum lipoferum ( Azospirillum lipoferum ), Azotobacter chroococcum ( Azotobacter chroococcum ), Azotobacter vinelandi ( Azotobacter vinelandii ), Bradyrhizobium japonicum , Brevibacillus brevis, Brevibacillus formosus , Brevibacillus formosus, Brevibacterium ammoniagenes , Brevibacterium pla Boom ( Brevibacterium flavum ), Brevibacterium linens ( Brevibacterium linens ), Brevibacterium otitidis ( Brevibacterium otitidis ), Burkholderia cepacia ( Burkholderia cepacia ), Burkholderia pyrosinia ( Burkholderia pyrrocinia ), Cellulomonas fimi ( Cellulomonas fimi ), Cellulomonas turbata ( Cellulomonas turbata ), Delftia acidovorans ( Delftia acidovorans ), Dietzia natronolimnaea ( Dietzia natronolimnaea ), Frateuria aurantia ( Frateuria aurantia ), Glomus intraradices , Helicosporium nizamabadense, Klebsiella mobilis , Lysinibacillus boronitolerans, Lysinibacillus pushpo Miss ( Lysinibacillus fusiformis ), Lysobacter antibioticus ( Lysobacter antibioticus ), Microbacterium aurum ( Microbacterium aurum ), Nocardiopsis dasonvillei ( Nocardiopsis dassonvillei ), Pessylomyces fumosoroseus ( Paecilomyces fumosoroseus ), Paecilomyces japonica, Paecilomyces lilacinus , Panibacillus amylolyticus , Paenibacillus azoreducens , Panibacillus chivensis ( Paenibacillus chibensis ), Panibacillus lentimorbus ( Paenibacillus lentimorbus ), Panibacillus macerans ( Paenibacillus macerans ), Panibacillus polymyxa ( Paenibacillus polymyxa ), Pantoea genus. S32 ( Pantoea sp. S32 ), Pediococcus Serevisiae ( Pediococcus cerevisiae ), Pediococcus halophilus ( Pediococcus halophilus ), Pedobactor ginseng ginsengisoli ( Pedobacter ginsengisoli ), Photorhabdus luminescens ( Photorhabdus luminescens ) . Sys ( Pseudomonas _ _ panipatensis ), Pseudomonas putida , Pseudomonas striata, Pseudomonas striata, Rhizopus delma , Rhizopus japonicus, Rhizopus oryzae, Tetrathiobacter kash One selected from the group consisting of Tetrathiobacter kashmirensis , Trichoderma hamatum , Trichoderma harzianum , and Variovorax boronicumulans , wherein the second microorganism is Bacillus amil Bacillus amyloliquefaciens , Bacillus brevis , Bacillus cereus , Bacillus circulans , Bacillus edaphicus , Bacillus glucanolyticus ), Bacillus licheniformis , Bacillus macerans, Bacillus mesonae , Bacillus mojavensis, Bacillus mucilaginosus , Bacillus natto ( Bacillus natto ), Bacillus polymyxa ( Bacillus polymyxa ), Bacillus pumilus ( Bacillus pumilus ), Bacillus subtilus ( Bacillus subtilis ), Bacillus bilis mortis ( Bacillus vallismortis ), Bacillus velezensis ( Bacillus velezensis ), Bacillus Megaterium ( Bacllius megaterium ), Bacillus macroides ( Bcillus macroides ), Streptomyces asoensis ( Streptomyces asoensis ), Streptomyces carpinensis ( Streptomyces carpinensis ), Streptomyces fradiae ( Streptomyces fradiae ), Streptomyces Seth griseo chromogejinseu ( Streptomyces griseochromogenes ), Streptomyces griseus ( Streptomyces griseus ), Streptomyces halstedii ( Streptomyces halstedii ), Streptomyces niger ( Streptomyces niger ), Streptomyces violaseus niger ( Streptomyces violaceusniger ), Rhodobacter azotoformans ( Rhodobacter azotoformans ), Rhodobacter capsulata ( Rhodobacter capsulata ), Rhodobacter capsulatus ( Rhodobacter capsulatus ), Rhodobacter rubrum ( Rhodobacter rubrum ), Rhodobacter spheroidis ( Rhodobacter sphaeroides ), Rhodopseudomonas capusulata ( Rhodopseudomonas capusulata ), Rhodopseudomonas palustris ( Rhodopseudomonas palustris ), Rhodopseudomonas Spheroides ( Rhodopseudomonas sphaeroides ), Rhodopseudomonas viridis ( Rhodopseudomonas viridis ), Saccharomyces amensis ( Saccharomyces anamensis ), Saccharomyces carlsbergensis ), Saccharomyces cerevisiae , Saccharomyces sake ( Saccharomyces sake ), Pichia anomala ( Pichia anomala ) and Pichia desticola ( Pichia deserticola ) and Pichia stipitis ( Pichia stipitis ), and the third microorganism is Acetobacter diazotrophicus ( Acetobacter diazotrophicus ), Acetobacter peroxidans ( Acetobacter peroxydans ), Acinetobacter Calcoaceticus ( Acinetobacter calcoaceticus ), Lactobacillus acidophilus ( Lactobacillus acidophilus ), Lactobacillus bulgaricus ( Lactobacillus bulgaricus ), Lactobacillus casei ( Lactobacillus casei ), Lactobacillus casei subsp . Lactobacillus confusa , Lactobacillus crispatus , Lactobacillus curvatus , Lactobacillus delbrueckii , Lactobacillus fermentum , Lactobacillus Helveticus, Lactobacillus paracasei, Lactobacillus perolens, Lactobacillus plantarum , Lactobacillus rhamnosus, Lactobacillus reuteri ), Lactobacillus salivarius , Lactococcus lactis, Stephanoascus ciferrii , Streptococcus cremoris , Streptococcus lactis , Streptococcus Streptococcus thermophilus , Aspergillus niger , Aspergillus oryzae , Candida kefir , Candida utilis and Candida valida ) It is characterized in that it is selected from the group consisting of.

In addition, in the present invention, the first macroelement mixture is composed of a mixture of 40 to 90% by weight of the first metabolite and 10 to 60% by weight of the first macroelement, and the second macroelement mixture is composed of 50 to 90% by weight of the second metabolite It is composed by mixing 95% by weight and 5 to 50% by weight of the second major element, and the trace element mixture is composed of 50.0 to 99.999% by weight of the third metabolite and 0.0001 to 50% by weight of the trace element. .

In addition, in the present invention, the step of forming the trace element mixture comprises the steps of mixing the third metabolite and the trace element to form a first mixture, and 0.1 to 15 parts by weight of an organic acid based on 100 parts by weight of the first mixture. It is characterized in that it comprises the step of adjusting the pH of the first mixture to 2.5 to 4.0 by adding part, and the step of chelating the pH-adjusted first mixture at 40 to 65 ° C. for 48 to 120 hours.

In addition, in the step of preparing the microbial metabolite fertilizer in the present invention, 70 to 90% by weight of the first macroelement mixture, 5 to 15% by weight of the second macroelement mixture, and 5 to 15% by weight of the trace element mixture are mixed. characterized by

In addition, in the present invention, the step of preparing the microbial metabolite fertilizer comprises 70 to 85% by weight of the first macroelement mixture, 5 to 10% by weight of the second macroelement mixture, and 5 to 10% by weight of the third macroelement mixture. And 5 to 10% by weight of the mixture of trace elements is characterized in that the mixture.

The present invention made as described above can significantly reduce the cost, manpower, and time for the treatment of the waste culture fluid (microbial metabolites) by using the waste culture fluid discarded after microbial culture as a raw material of fertilizer, and the components contained in the metabolites It is possible to provide the effect of promoting plant growth, improving soil fertility and decomposition of organic matter, and improving nutrient absorption and enhancing antioxidant efficacy. In addition, by giving various nutrients to plants by actively utilizing various macroelements and microelements, the effect of improving yield can be expected by rapidly supplying nutrients to the roots of fruits or crops.

1 is a manufacturing process diagram of a microbial metabolite fertilizer using waste culture medium discarded after microbial culture according to the present invention.
Figure 2 is a manufacturing process diagram of the trace element mixture according to the present invention.
Figure 3 is a manufacturing process diagram of a microbial metabolite fertilizer using waste culture medium discarded after microbial culture according to another embodiment of the present invention.

In describing the present invention, the advantages and features of the present invention, and methods for achieving them will become clear with reference to the embodiments described below in detail in conjunction with the accompanying drawings. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided so that the disclosed content will be thorough and complete and the spirit of the present invention will be sufficiently conveyed to those skilled in the art. In the drawings, the thickness of layers and regions is exaggerated for clarity.

Terms such as first and second may be used to describe various components, but the components are not limited by the terms. These terms are only used for the purpose of distinguishing one component from another. For example, a first element could be termed a second element, and, similarly, a second element could be termed a first element, without departing from the scope of the present invention.

Terms such as top, bottom, top, bottom, or top, bottom are used to distinguish the relative positions of components. For example, in the case of naming the upper part in the drawing as the upper part and the lower part in the drawing as the lower part for convenience, in practice, the upper part may be named as the lower part and the lower part may be named as the upper part without departing from the scope of the present invention. .

Terms used in this application are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly dictates otherwise. In this application, the terms "include" or "have" are intended to designate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, but one or more other features It should be understood that it does not preclude the possibility of the presence or addition of numbers, steps, operations, components, parts, or combinations thereof.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs. Terms such as those defined in commonly used dictionaries should be interpreted as having meanings consistent with the meanings in the context of the related art, and unless explicitly defined in this application, they should not be interpreted in ideal or excessively formal meanings. don't

Hereinafter, with reference to the accompanying drawings, a method for producing a microbial metabolite fertilizer using a waste culture medium discarded after microbial culture according to the present invention will be described in detail.

1 is a manufacturing process diagram of a microbial metabolite fertilizer using waste culture medium discarded after culturing microorganisms according to an embodiment of the present invention. As shown in FIG. 1, the present invention includes a first macroelement mixture composition step (S10), It includes a major element mixture composition step (S20), a trace element mixture composition step (S30), and a microbial metabolite fertilizer composition step (S50).

In the first multi-element mixture composition step 10, the culture medium is inoculated with the first microorganism, then cultured in a large amount in an incubator, and the culture medium in the incubator is centrifuged in a centrifuge to separate the first microorganism and the first metabolite. Next, a step of recovering the first metabolite and mixing it with the first macro element to form a first macro element mixture.

In the present invention, the first microorganism is Agrobacterium sp ., Alcaligenes defragrans , Alcaligenes piechaudii , Ampelomyces quisqualis ), Arthrobacter protophormiae ), Arthrobotrys oligospora , Azospirillum brasilense , Azospirillum lipoferum , Azotobacter crococombe ( Azotobacter chroococcum ), Azotobacter vinelandii ( Azotobacter vinelandii ), Bradyrhizobium japonicum ( Bradyrhizobium japonicum ), Brevibacillus brevis ( Brevibacillus brevis ), Brevibacillus formosus ( Brevibacillus formosus ), Brevibacterium ammonia Genes ( Brevibacterium ammoniagenes ), Brevibacterium flavum ( Brevibacterium flavum ), Brevibacterium linens ( Brevibacterium linens ), Brevibacterium otitidis ( Brevibacterium otitidis ), Burkholderia cepacia , Burkholderia pyrrocinia, Cellulomonas fimi , Cellulomonas turbata , Delftia acidovorans , Dietzia natronolimnaea , Frateuria aurantia, Glomus intraradices , Helicosporium nizamabadense, Klebsiella mobilis , Ricinibacillus Boroni Lysinibacillus boronitolerans, Lysinibacillus fusiformis , Lysobacter antibioticus, Microbacterium aurum , Nocardiopsis dassonvillei , Paecilomyces fumosoroseus, Paecilomyces japonica, Paecilomyces lilacinus , Paenibacillus amylolyticus , Panibacillus azo Paenibacillus azoreducens ), Paenibacillus chibensis , Paenibacillus lentimorbus , Paenibacillus macerans , Paenibacillus polymyxa , Pantoea genus. S32 ( Pantoea sp. S32 ), Pediococcus Serevisiae ( Pediococcus cerevisiae ), Pediococcus halophilus ( Pediococcus halophilus ), Pedobactor ginseng ginsengisoli ( Pedobacter ginsengisoli ), Photorhabdus luminescens ( Photorhabdus luminescens ) . Sys ( Pseudomonas _ _ panipatensis ), Pseudomonas putida , Pseudomonas striata, Pseudomonas striata, Rhizopus delma , Rhizopus japonicus, Rhizopus oryzae, Tetrathiobacter kash It may be one selected from the group consisting of Tetrathiobacter kashmirensis , Trichoderma hamatum, Trichoderma harzianum , and Variovorax boronicumulans .

The first metabolite is a liquid by-product formed in the process of culturing and separating one first microorganism selected from among the plurality of microorganisms described above, and when added as a raw material for fertilizer, plant growth promotion, nitrogen fixation, phosphoric acid solubilization, potassium solubilization effect, etc.

The first macroelement may be one or more selected from the group consisting of nitrogen (N), phosphoric acid (P), and potassium (K). Nitrogen (N) is the main component of protein that makes cells and plays a major role in growth, and is especially necessary for leafy vegetables and root vegetables. Phosphoric acid (P) is a major component of the nucleus of cells and is necessary for normal cell activity. Early growth is poor. Potassium (K) is necessary for photosynthesis or protein synthesis from amino acids, and plays a role in helping plants physiology and strengthening tissues. In this case, brown spots appear on the leaves, the tips of the leaves become red, and they become vulnerable to drought or pests.

According to an embodiment of the present invention, the first macroelement mixture may be a nitrogenous liquid fertilizer formed by mixing 90 to 110 parts by weight of nitrogen (N) with respect to 90 to 100 parts by weight of the recovered first metabolite, or the It may be a phosphoric acid liquid fertilizer composed by mixing 40 to 60 parts by weight of phosphoric acid with respect to 40 to 60 parts by weight of the first metabolite, or 40 to 60 parts by weight of potassium (K) based on 40 to 60 parts by weight of the first metabolite. It may be a mixed potassium liquid fertilizer.

Here, since nitrogen (N), phosphoric acid (P), and potassium (P) are important components that influence the growth of plants, the first multi-element is to input all three types so that nutrients necessary for crop growth are evenly supplied. Accordingly, the first macroelement mixture may be composed of a mixture of 40 to 90% by weight of the first metabolite and 10 to 60% by weight of the first macroelement, for example, 50% by weight of the first metabolite %, nitrogen (N) 30% by weight, phosphoric acid (P) 10% by weight, and potassium (K) 10% by weight may be mixed.

Next, in the second multi-element mixture composition step (S20), the culture medium is inoculated with the second microorganism, then cultured in a large amount in an incubator, and the culture medium in the incubator is centrifuged in a centrifuge to separate the second microorganism and the second metabolite. After separation, the second metabolite is recovered and mixed with the second major element to form a second major element mixture.

In the present invention, the second microorganism is Bacillus amyloliquefaciens , Bacillus brevis, Bacillus cereus , Bacillus circulans , Bacillus edaphicus , Bacillus glucanolyticus , Bacillus licheniformis, Bacillus macerans , Bacillus mesonae , Bacillus mojavensis , Bacillus radish Bacillus mucilaginosus , Bacillus natto , Bacillus polymyxa, Bacillus pumilus, Bacillus subtilis , Bacillus bilis mortis, Bacillus vallismortis , Bacillus velezensis , Bacillus megaterium , Bacillus macroides , Streptomyces asoensis , Streptomyces carpinensis , Streptomyces Streptomyces fradiae , Streptomyces griseochromogenes, Streptomyces griseochromogenes , Streptomyces griseus, Streptomyces halstedii , Streptomyces niger ), Streptomyces violaceus niger ( Streptomyces violaceusniger ), Rhodobacter azotoformans ( Rhodobacter azotoformans ), Rhodobacter capsulata ( Rhodobacter capsulata ), Rhodobacter capsulatus ( Rhodobacter capsulatus ), Rhodobacter rubrum ( Rhodobacter rubrum ), Rhodobacter sphaeroides ( Rhodobacter sphaeroides ), Rhodopseudomonas capusurata ( Rhodopseudomonas capusulata ), Rhodopseudomonas palustris ( Rhodopseudomonas palustris ), Rhodopseudomonas sphaeroides ( Rhodopseudomonas sphaeroides ), Rhodopseudomonas viridis ( Rhodopseu domonas viridis ) , Saccharomyces Amensis ( Saccharomyces anamensis ), Saccharomyces Carlsbergensis ( Saccharomyces carlsbergensis ), Saccharomyces cerevisiae ( Saccharomyces cerevisiae ), Saccharomyces sake ( Saccharomyces sake ), Pichia Anomala ( Pichia anomala ), Pichia desticola ( Pichia deserticola ), and Pichia stipitis ( Pichia stipitis ).

The second metabolite is a metabolite produced in the fermentation and culturing process of one microorganism selected from among the plurality of microorganisms described above, and is added as a raw material for fertilizer to increase soil fertility and to secrete alkaline degrading enzymes to produce proteins, It promotes the decomposition and fermentation of organic matter such as fiber to decompose phosphoric acid and nitric acid compounds and remove harmful gases, and provides sterilization and insecticidal effects and improvement of the livestock environment.

The second macroelement may be one or more selected from the group consisting of calcium (Ca), magnesium (Mg), and sulfur (S). Calcium (Ca) is the fourth most essential element after nitrogen, phosphorus, and potassium among 16 plant essential nutrients. Sufficient calcium is required to form strong cell membranes and harvest hard quality crops with good color. there is. In addition, it neutralizes acidic soil to inhibit the absorption of heavy metals, promotes the decomposition of organic matter to improve soil chemistry, and promotes microbial activity to improve soil structure. When calcium is insufficient, various physiological disorders occur in crops, and growth is inhibited, resulting in reduced leaf size and thin stems. In addition, if the concentration of salt in the soil is high and the level of cations in the fertilizer is high, calcium is not absorbed well, and the dryness and strong sunlight are likely to cause calcium deficiency in crops. do. Magnesium (Mg) improves taste and quality, sulfur (S) increases productivity and health, improves crop protein, fat (oil) production, vitamin formation, nutrient movement and aroma, taste, color, and sugar content within the crop body acts to increase

According to an embodiment of the present invention, the second macroelement mixture may be a calcium liquid fertilizer composed of a mixture of 50 to 95% by weight of the recovered second metabolite and 5 to 50% by weight of calcium (Ca), or It may be a magnesium liquid fertilizer composed of a mixture of 50 to 95% by weight of the second metabolite and 5 to 50% by weight of magnesium (Mg). In addition, at least two or more of the second macroelements may be added to facilitate plant growth. For example, the second macroelement mixture contains 77% by weight of the second metabolite and 18% of calcium (Ca). It may be composed by mixing 5% by weight of magnesium (MG) and 5% by weight.

Next, in the trace element mixture composition step (S30), the culture medium is inoculated with the third microorganism, then cultured in a large amount in an incubator, and the culture medium in the incubator is centrifuged in a centrifuge to separate the third microorganism and the third metabolite. Then, the third metabolite is recovered, and among the group consisting of zinc (Zn), iron (Fe), boron (B), copper (Cu), manganese (Mn), silicic acid, and molybdenum (Mo) This is a step of forming a trace element mixture by mixing with one or more selected trace elements.

First, in the present invention, the third microorganisms are Acetobacter diazotrophicus, Acetobacter peroxydans, Acinetobacter calcoaceticus , Lactobacillus acidophilus ( Lactobacillus acidophilus ), Lactobacillus bulgaricus , Lactobacillus casei, Lactobacillus casei subspecies rhamnosus, Lactobacillus casei subsp rhamnosus , Lactobacillus confusa, Lactobacillus crispatus crispatus ), Lactobacillus curvatus ( Lactobacillus curvatus ), Lactobacillus delbruecki ( Lactobacillus delbrueckii ), Lactobacillus fermentum ( Lactobacillus fermentum ), Lactobacillus helveticus ( Lactobacillus helveticus ), Lactobacillus paracasei ( Lactobacillus paracasei ) , Lactobacillus perolens, Lactobacillus plantarum , Lactobacillus rhamnosus , Lactobacillus reuteri , Lactobacillus salivarius, Lactobacillus salivarius , Lactobacillus lactis ( Lactococcus lactis ), Stephanoascus ciferrii, Streptococcus cremoris , Streptococcus lactis , Streptococcus thermophilus , Aspergillus niger ), Aspergillus oryzae, Candida kefir , Candida utilis, and Candida valida .

The third metabolite is a by-product formed in the process of culturing and separating one third microorganism selected from among the plurality of microorganisms described above, and is added as a raw material for fertilizer to form a mycelium on the root in the process of cultivating the plant to grow the root It protects crops, helps absorb nutrients, promotes continuous differentiation and growth of roots, and facilitates plant rooting even in hot and humid soil. In addition, it provides plant growth promotion, fruit uniformity, crop freshness, water vapor effect, hypertrophy effect, and root vitality increase effect. In addition, it promotes the growth of useful microorganisms by suppressing acidity in the soil, provides growth inhibitory effects against various putrefactive and pathogenic microorganisms, generates antibiotics (bacteriocin), promotes seed germination and root development, and organic acids is produced and chelated with the cation of the insoluble phosphoric acid compound to dissolve phosphoric acid.

The trace elements supply trace metals that can be contained in the plant body to control the rooting and growth of plants, such as zinc (Zn), iron (Fe), boron (B), copper (Cu), manganese (Mn ), silicic acid, and may be one or more selected from the group consisting of molybdenum (Mo). Although the trace element is a very small amount, it is an essential element for the growth of crops. For example, boron (B) helps the sugar photosynthesized from the leaves of plants to flow to fruits, branches and roots, and pollen germination during flowering and fertilization It promotes elongation of pollen tubes and pollen tubes to help increase fruiting rate. Copper (Cu) is related to the production of chlorophyll and binds to chlorophyll to stabilize and protect it. Silicic acid is deposited in the epidermal cells of leaves or stems. It is silicified to improve the yield and quality of crops and increases stress resistance. It is non-toxic and imparts the function of neutralizing various harmful components to plants. Molybdenum (Mo) fixes nitrogen when forming crop proteins. It plays a role in improving resistance to viruses.

According to an embodiment of the present invention, the trace element mixture is a chelated zinc trace element fertilizer composed of a mixture of 70 to 99.95% by weight of the third metabolite and 0.05 to 30% by weight of zinc (Zn), or 80 to 99.9% by weight of the third metabolite. It may be a chelated iron trace element fertilizer composed of a mixture of 0.1 to 20% by weight of iron (Fe). Alternatively, the trace element mixture is 50 to 99.9999% by weight of the third metabolite, and one or more trace amounts selected from boron (B), copper (Cu), manganese (Mn), silicic acid, or molybdenum (Mo) It may be a trace element complex fertilizer composed of a mixture of 0.0001 to 50% by weight of elements.

Preferably, the trace element is a very small amount, but since it is an essential element for the growth of crops, it is preferable to mix at least two or more kinds. 0.05% by weight of zinc (Zn), 0.1% by weight of iron (Fe), 0.05% by weight of copper (Cu), 0.05% by weight of boron (B), 0.1% by weight of manganese (Mn), and silicic acid 5% by weight and 0.0005% by weight of molybdenum (Mo) may be mixed.

Next, as shown in FIG. 2, the step of preparing the trace element mixture according to the present invention (S30) includes the step of forming a first mixture by mixing the third metabolite and the trace element (S31), and adding an organic acid to the first mixture. It may include adjusting the pH by adding (S32), and chelating the pH-adjusted first mixture (S33).

First, chelation means that minerals are combined as if they are caught with a claw by a ligand. Chelate chemical bond refers to a state in which a chelating agent, an organic molecule with special properties, surrounds a metal element and forms a heterocyclic structural bond on both sides as if clamped with tongs.

Most of the microorganisms constituting the third microorganisms are lactic acid bacteria, and metabolites of these lactic acid bacteria include various organic acids such as lactic acid that serve as a chelating agent. It is converted into trace elements and can rapidly increase the absorption rate of trace elements by plants due to the binding action of surface ligands.

This increase in the absorption rate of trace elements increases the nutrient availability of plants and at the same time reduces the remaining insoluble nutrients in the soil, preventing and reducing salt accumulation even with continuous use.

According to a preferred embodiment, the trace element mixture is a mixture of 50 to 99.9999% by weight of the third metabolite and 0.0001 to 50% by weight of the trace element to form a first mixture (S31), based on 100 parts by weight of the first mixture After adding 0.1 to 15 parts by weight of an organic acid to adjust the pH to 2.5 to 4.0 (S32), the pH-adjusted first mixture is chelated at 40 to 65 ° C. for 48 to 120 hours (S33). As for trace elements, a binding reaction between organic acids such as lactic acid and inorganic trace elements in metabolites occurs under the condition of pH 4 or less, and organic acids are preferably added for a more stable chelation reaction.

The trace element mixture prepared by this process has remarkable effects compared to existing chemical fertilizers in terms of increased nutrient absorption rate and antioxidant effect. However, it is possible to utilize the soil improvement characteristics of lactic acid, a metabolite of lactic acid bacteria, and to maximize the efficiency of nutrient use by actively utilizing the characteristics of lactic acid, which has a relatively low molecular weight and is easily soluble in water.

Next, the microbial metabolite fertilizer composition step (S50) according to an embodiment of the present invention is to mix the first macro element mixture, the second macro element mixture, and the trace element mixture to form a microbial metabolite fertilizer As a step, preferably, the microbial metabolite fertilizer is prepared by mixing 70 to 90% by weight of the first macroelement mixture, 5 to 15% by weight of the second macroelement mixture, and 5 to 15% by weight of the trace element mixture. can If the content of any one of the first major element mixture, the second major element mixture, and the minor element mixture is less than the standard value, a deficiency phenomenon occurs due to a lack of components, which is not preferable.

In addition, if necessary, a microbial metabolite fertilizer may be formed by mixing two types selected from the first macro element mixture, the second macro element mixture, and the trace element mixture.

On the other hand, in the present invention, MRS broth medium can be used as a medium for inoculating and fermenting the first, second and third microorganisms, or a medium containing a carbon source, a nitrogen source, vitamins and minerals, or seawater-derived mineral enrichment. A medium to which one or more of water, sulfur aqueous dispersion, and illite aqueous dispersion is added may be used. In addition, the first microorganism, the second microorganism, and the third microorganism may be cultured under appropriate culture conditions to enable smooth growth, and the culture conditions are not limited to the scope of the present invention. For example, the culture conditions of the first microorganism may be cultured for 24 to 48 hours at 25 to 40 ° C, an air injection amount of 0.2 to 0.4 vvm, and a rotational speed of 50 to 200 rpm, and the culture condition of the second microorganism is 25 to 50 ° C. , It can be cultured for 24 to 48 hours at an air injection amount of 0.2 to 0.4 vvm and a rotation speed of 50 to 200 rpm. It can be incubated for 48 hours.

Example 1.

The culture medium was inoculated with Paenibacillus polymyxa and then cultured for 24 hours at 28° C., air injection amount 0.3vvm, and rotation speed 180 rpm in the first incubator. Thereafter, the culture medium in the first incubator was centrifuged in a centrifuge to separate Paenibacillus polymyxa and the first metabolite, and then the first metabolite was recovered. Thereafter, 50% by weight of the first metabolite, 10% by weight of nitrogen (N), 10% by weight of phosphoric acid (P), and 30% by weight of potassium (K) were mixed to form a first macroelement mixture.

Next, Bacillus subtilis was inoculated into the medium, and then cultured in a second incubator at 28° C., an air injection amount of 0.3 vvm, and a rotation speed of 180 rpm for 24 hours. Thereafter, the culture medium in the second incubator was centrifuged in a centrifuge to separate Bacillus subtilis and the second metabolite, and the second metabolite was recovered. Thereafter, 94% by weight of the second metabolite, 3.5% by weight of calcium (Ca), and 2.5% by weight of magnesium (Mg) were mixed to form a second major element mixture.

Next, the medium was inoculated with Lactobacillus bulgaricus , and then cultured for 24 hours at 37° C., an air injection amount of 0.3vvm, and a rotation speed of 180 rpm in a third incubator. Thereafter, the culture solution of the third incubator was centrifuged in a centrifuge to separate Lactobacillus bulgaricus and a third metabolite, and the third metabolite was recovered. Then, 94.6495% by weight of the third metabolite, 0.05% by weight of zinc (Zn), 0.1% by weight of iron (Fe), 0.05% by weight of copper (Cu), 0.05% by weight of boron (B), and manganese ( Mn) 0.1% by weight, silicic acid 5% by weight, and molybdenum (Mo) 0.0005% by weight are mixed to form a first mixture, and then 10 parts by weight of an organic acid is added to 100 parts by weight of the first mixture to pH was adjusted to 3.0, and then the pH-adjusted first mixture was chelated at 55° C. for 24 hours to form a trace element mixture.

Thereafter, 85% by weight of the first major element mixture, 10% by weight of the second major element mixture, and 5% by weight of the trace element mixture were mixed to form a microbial metabolite fertilizer according to an embodiment of the present invention.

Next, Figure 3 is a manufacturing process diagram of a microbial metabolite fertilizer using waste culture medium discarded after culturing microorganisms according to another embodiment of the present invention, the present invention includes a first large element mixture composition step (S10), and a second large amount It may include an element mixture composition step (S20), a trace element mixture composition step (S30), a third major element mixture composition step (S40), and a microbial metabolite fertilizer preparation step (S50). Here, the first major element mixture composition step (S10), the second major element mixture composition step (S20), and the trace element mixture composition step (S30) correspond to the process in one embodiment of the present invention described above. , detailed descriptions are omitted below.

According to another embodiment of the present invention, in the third major element mixture forming step (S40), the third metabolite through the trace element mixture forming step (S30) and the second major element are mixed, and then an organic acid is added. This is a step of adjusting the pH and then performing a chelation reaction to form a mixture of the third major element.

Preferably, the third major element mixture is a mixture of 50 to 95% by weight of the third metabolite and 5 to 50% by weight of the second macroelement to form a second mixture, and then 100 parts by weight of the second mixture 0.1 to 15 parts by weight of an organic acid is added to adjust the pH to 2.5 to 4.0, and the pH-adjusted second mixture is chelated at 40 to 65 ° C. for 48 to 120 hours.

As described above, in the metabolites of lactic acid bacteria, which are most of the microorganisms constituting the third microorganisms, there are various organic acids such as lactic acid that serve as a chelating agent, and these organic acids are chelated with inorganic second major elements. is converted into the second macroelement in the form of organic matter, thereby rapidly increasing the absorption rate of the second macroelement in plants due to the binding action of surface ligands. Various effects can be expected, such as promoting physiological activity, increasing soil fertility, improving the decomposition of organic matters, and solubilizing nutrients.

In addition, in the step of preparing the microbial metabolite fertilizer according to another embodiment of the present invention (S50), 70 to 85% by weight of the first macroelement mixture, 5 to 10% by weight of the second macroelement mixture, and the third macroelement mixture 5 to 10% by weight of the major element mixture and 5 to 10% by weight of the trace element mixture may be mixed.

If the content of any one of the first major element mixture, the second major element mixture, the third major element mixture, and the trace element mixture is less than the standard value, a deficiency phenomenon occurs due to a lack of components, which is not preferable.

Example 2.

The medium was inoculated with Azospirillum lipoferum as a first microorganism, and then cultured for 36 hours in a first incubator at 28° C., an air injection amount of 0.3 vvm, and a rotation speed of 180 rpm. Thereafter, the culture medium in the first incubator was centrifuged in a centrifuge to separate Azospirillum lipoferum and the first metabolite, and then the first metabolite was recovered. Thereafter, 50% by weight of the first metabolite, 30% by weight of nitrogen (N), 10% by weight of phosphoric acid (P), and 10% by weight of potassium (K) were mixed to form a first macroelement mixture.

Next, the medium was inoculated with Bacillus megaterium as a second microorganism, and then cultured for 36 hours in a second incubator at 28° C., an air injection amount of 0.3 vvm, and a rotation speed of 180 rpm. Thereafter, the culture medium in the second incubator was centrifuged in a centrifuge to separate Bacillus megaterium and the second metabolite, and the second metabolite was recovered. Thereafter, 77% by weight of the second metabolite, 18% by weight of calcium (Ca), and 5% by weight of magnesium (Mg) were mixed to form a second major element mixture.

Next, the medium was inoculated with Lactobacillus plantarum as a third microorganism, and then cultured in a third incubator at 28° C., an air injection amount of 0.3 vvm, and a rotation speed of 180 rpm for 36 hours. Thereafter, the culture solution of the third incubator was centrifuged in a centrifuge to separate Lactobacillus plantarum and the third metabolite, and the third metabolite was recovered. Then, 94.6495% by weight of the third metabolite, 0.05% by weight of zinc (Zn), 0.1% by weight of iron (Fe), 0.05% by weight of copper (Cu), 0.05% by weight of boron (B), and manganese ( Mn) 0.1% by weight, silicic acid 5% by weight, and molybdenum (Mo) 0.0005% by weight are mixed to form a first mixture, and then 10 parts by weight of an organic acid is added to 100 parts by weight of the first mixture to pH was adjusted to 3.0, and then the pH-adjusted first mixture was subjected to a chelation reaction at 65° C. for 48 hours to form a mixture of trace elements.

Next, a second composition was prepared by mixing 77% by weight of the third metabolite, 18% by weight of calcium (Ca), and 5% by weight of magnesium (Mg), and then, with respect to 100 parts by weight of the second mixture, organic acid 10 The pH was adjusted to 3.0 by adding parts by weight, and the pH-adjusted second mixture was subjected to a chelation reaction at 65° C. for 48 hours to form a third large element mixture.

Thereafter, 80% by weight of the first major element mixture, 10% by weight of the second major element mixture, 5% by weight of the trace element mixture, and 5% by weight of the third major element mixture were mixed, according to another embodiment of the present invention. A microbial metabolite fertilizer was formulated.

The present invention made as described above can reduce the cost, manpower, and time for the treatment of metabolites by recycling waste-treated microbial metabolites as raw materials for liquid fertilizer, and can improve salt accumulation and denitrification by utilizing active ingredients of microorganisms. suppression, solubilization of insoluble nutrients in soil, soil improvement, increase in nutrient availability, suppression of greenhouse gas generation and sterilization, insecticidal effect, etc. Considering the description, it will be possible to sufficiently change, transform, substitute, and replace, and is not limited to the above-described embodiment.

Claims (6)

The first microorganism is inoculated into the medium, then cultured in a large amount in an incubator, the culture medium in the incubator is centrifuged in a centrifuge to separate the first microorganism and the first metabolite, and then the first metabolite is recovered to nitrogen ( N), mixing with one or more first macro elements selected from the group consisting of phosphoric acid (P) and potassium (K) to form a first macro element mixture (S10);
After inoculating the second microorganism in the medium, it is cultured in a large amount in an incubator, and the culture medium in the incubator is centrifuged in a centrifuge to separate the second microorganism and the second metabolite, and then the second metabolite is recovered and calcium ( forming a second major element mixture by mixing with one or more second major elements selected from the group consisting of Ca), magnesium (Mg), and sulfur (S) (S20);
After inoculating the medium with a third microorganism, it is cultured in a large amount in an incubator, the culture medium in the incubator is centrifuged in a centrifuge to separate the third microorganism and a third metabolite, and then the third metabolite is recovered and zinc ( A mixture of trace elements by mixing one or more trace elements selected from the group consisting of Zn), iron (Fe), boron (B), copper (Cu), manganese (Mn), silicic acid, and molybdenum (Mo) Forming a step (S30); and;
Mixing the first major element mixture, the second major element mixture, and the trace element mixture to form a microbial metabolite fertilizer (S50); including,
The first microorganism is Agrobacterium sp. , Alcaligenes defragrans , Alcaligenes piechaudii , Ampelomyces quisqualis , Aspen Robacter protophormiae ( Arthrobacter protophormiae ), Arthrobotrys oligospora ( Arthrobotrys oligospora ), Azospirillum brasilense ( Azospirillum brasilense ), Azospirillum lipoferum ( Azospirillum lipoferum ), Azotobacter chroococcum ), Azotobacter vinelandii, Bradyrhizobium japonicum , Brevibacillus brevis, Brevibacillus formosus, Brevibacillus formosus , Brevibacterium ammoniagenes ( Brevibacterium ammoniagenes ), Brevibacterium flavum, Brevibacterium flavum , Brevibacterium linens , Brevibacterium otitidis , Burkholderia cepacia , Burkholderia Leah pyrosinia ( Burkholderia pyrrocinia ), Cellulomonas fimi ( Cellulomonas fimi ), Cellulomonas turbata ( Cellulomonas turbata ), Delftia acidovorans ( Delftia acidovorans ), Dietzia natronolimnaea ( Dietzia natronolimnaea ), prate Uriah Aurantia ( Frateuria aurantia ), Glomus intraradices ( Glomus intraradices ), Helicosporium nizamabadense ( Helicosporium nizamabadense ), Klebsiella mobilis ( Klebsiella mobilis ), Ricinibacillus Voronitollerans ( Lysinibacillus boronitolerans), Lysinibacillus fusiformis , Lysobacter antibioticus, Microbacterium aurum , Nocardiopsis dassonvillei , Pessilo Myces fumosoroseus ( Paecilomyces fumosoroseus ), Paecilomyces japonica ( Paecilomyces japonica ), Paecilomyces lilacinus ( Paecilomyces lilacinus ), Panibacillus amylolyticus ( Paenibacillus amylolyticus ), Panibacillus azoreducens ( Paenibacillus azoreducens ), Paenibacillus chibensis , Paenibacillus lentimorbus , Paenibacillus macerans , Paenibacillus polymyxa , Pantoea genus. S32 ( Pantoea sp. S32 ), Pediococcus Serevisiae ( Pediococcus cerevisiae ), Pediococcus halophilus ( Pediococcus halophilus ), Pedobactor ginseng ginsengisoli ( Pedobacter ginsengisoli ), Photorhabdus luminescens ( Photorhabdus luminescens ) . Sys ( Pseudomonas _ _ panipatensis ), Pseudomonas putida , Pseudomonas striata, Pseudomonas striata, Rhizopus delma , Rhizopus japonicus, Rhizopus oryzae, Tetrathiobacter kash One selected from the group consisting of Tetrathiobacter kashmirensis , Trichoderma hamatum , Trichoderma harzianum , and Variovorax boronicumulans ,
The second microorganism is Bacillus amyloliquefaciens , Bacillus brevis, Bacillus cereus , Bacillus circulans , Bacillus edaphicus , Bacillus Bacillus glucanolyticus, Bacillus licheniformis , Bacillus macerans , Bacillus mesonae , Bacillus mojavensis , Bacillus Musillajinosus ( Bacillus mucilaginosus ), Bacillus natto ( Bacillus natto ), Bacillus polymyxa ( Bacillus polymyxa ), Bacillus pumilus ( Bacillus pumilus ), Bacillus subtilus ( Bacillus subtilis ), Bacillus bilis mortis ( Bacillus vallismortis ), Bacillus bellegen Cis ( Bacillus velezensis ), Bacillus megaterium ( Bacllius megaterium ), Bacillus macroides ( Bcillus macroides ), Streptomyces asoensis ( Streptomyces asoensis ), Streptomyces carpinensis ( Streptomyces carpinensis ), Streptomyces pradiae ( Streptomyces fradiae ), Streptomyces griseochromogenesu ( Streptomyces griseochromogenes ), Streptomyces griseus ( Streptomyces griseus ), Streptomyces halstedii ( Streptomyces halstedii ), Streptomyces niger ( Streptomyces niger ), streptomyces Myces violaceus niger ( Streptomyces violaceusniger ), Rhodobacter azotoformans ( Rhodobacter azotoformans ), Rhodobacter capsulata ( Rhodobacter capsulata ), Rhodobacter capsulatus ( Rhodobacter capsulatus ), Rhodobacter rubrum ( Rhodobacter rubrum ), Rhodobacter sphaeroides ( Rhodobacter sphaeroides ), Rhodopseudomonas capusurata ( Rhodopseudomonas capusulata ), Rhodopseudomonas palustris ( Rhodopseudomonas palustris ), Rhodopseudomonas sphaeroides ( Rhodopseudomonas sphaeroides ), Rhodopseudomonas viridis ( Rhodopseudomonas viri dis ), sacaro Saccharomyces anamensis , Saccharomyces carlsbergensis, Saccharomyces cerevisiae , Saccharomyces sake , Pichia anomala ) And Pichia desticola ( Pichia deserticola ), Pichia stipitis ( Pichia stipitis ) Is one selected from the group consisting of,
The third microorganism is Acetobacter diazotrophicus, Acetobacter peroxydans, Acinetobacter calcoaceticus , Lactobacillus acidophilus, Lactobacillus acidophilus Bacillus bulgaricus, Lactobacillus casei, Lactobacillus casei subsp rhamnosus, Lactobacillus confusa , Lactobacillus crispatus , Lactobacillus Lactobacillus curvatus , Lactobacillus delbrueckii , Lactobacillus fermentum , Lactobacillus helveticus, Lactobacillus paracasei , Lactobacillus ferolens ( Lactobacillus perolens ), Lactobacillus plantarum ( Lactobacillus plantarum ), Lactobacillus rhamnosus ( Lactobacillus rhamnosus ), Lactobacillus reuteri ( Lactobacillus Reuteri ), Lactobacillus salivarius ( Lactobacillus salivarius ), Lactococcus lactis ( Lactococcus lactis ), Stefano Stephanoascus ciferrii , Streptococcus cremoris , Streptococcus lactis , Streptococcus thermophilus , Aspergillus niger , Aspergyl Loose oryzae ( Aspergillus oryzae ), Candida kefir ( Candida kefir ), Candida utilis ( Candida utilis ) and Candida valida ( Candida valida ) Utilization of lung culture medium discarded after culturing microorganisms, characterized in that one selected from the group consisting of A method for producing a microbial metabolite fertilizer.
The first microorganism is inoculated into the medium, then cultured in a large amount in an incubator, the culture medium in the incubator is centrifuged in a centrifuge to separate the first microorganism and the first metabolite, and then the first metabolite is recovered to nitrogen ( N), mixing with one or more first macro elements selected from the group consisting of phosphoric acid (P) and potassium (K) to form a first macro element mixture (S10);
After inoculating the second microorganism in the medium, it is cultured in a large amount in an incubator, and the culture medium in the incubator is centrifuged in a centrifuge to separate the second microorganism and the second metabolite, and then the second metabolite is recovered and calcium ( forming a second major element mixture by mixing with one or more second major elements selected from the group consisting of Ca), magnesium (Mg), and sulfur (S) (S20);
After inoculating the medium with a third microorganism, it is cultured in a large amount in an incubator, the culture medium in the incubator is centrifuged in a centrifuge to separate the third microorganism and a third metabolite, and then the third metabolite is recovered and zinc ( A mixture of trace elements by mixing one or more trace elements selected from the group consisting of Zn), iron (Fe), boron (B), copper (Cu), manganese (Mn), silicic acid, and molybdenum (Mo) Forming a step (S30) and;
mixing the recovered third metabolite and the second macroelement, adjusting the pH by adding an organic acid, and then performing a chelation reaction to prepare a third macroelement mixture (S40);
Forming a microbial metabolite fertilizer by mixing the first macro element mixture, the second macro element mixture, the third macro element mixture, and the trace element mixture (S50); including,
The first microorganism is Agrobacterium sp. , Alcaligenes defragrans , Alcaligenes piechaudii , Ampelomyces quisqualis , Aspen Robacter protophormiae ( Arthrobacter protophormiae ), Arthrobotrys oligospora ( Arthrobotrys oligospora ), Azospirillum brasilense ( Azospirillum brasilense ), Azospirillum lipoferum ( Azospirillum lipoferum ), Azotobacter chroococcum ), Azotobacter vinelandii, Bradyrhizobium japonicum , Brevibacillus brevis, Brevibacillus formosus, Brevibacillus formosus , Brevibacterium ammoniagenes ( Brevibacterium ammoniagenes ), Brevibacterium flavum, Brevibacterium flavum , Brevibacterium linens , Brevibacterium otitidis , Burkholderia cepacia , Burkholderia Leah pyrosinia ( Burkholderia pyrrocinia ), Cellulomonas fimi ( Cellulomonas fimi ), Cellulomonas turbata ( Cellulomonas turbata ), Delftia acidovorans ( Delftia acidovorans ), Dietzia natronolimnaea ( Dietzia natronolimnaea ), prate Uriah Aurantia ( Frateuria aurantia ), Glomus intraradices ( Glomus intraradices ), Helicosporium nizamabadense ( Helicosporium nizamabadense ), Klebsiella mobilis ( Klebsiella mobilis ), Ricinibacillus Voronitollerans ( Lysinibacillus boronitolerans), Lysinibacillus fusiformis , Lysobacter antibioticus, Microbacterium aurum , Nocardiopsis dassonvillei , Pessilo Myces fumosoroseus ( Paecilomyces fumosoroseus ), Paecilomyces japonica ( Paecilomyces japonica ), Paecilomyces lilacinus ( Paecilomyces lilacinus ), Panibacillus amylolyticus ( Paenibacillus amylolyticus ), Panibacillus azoreducens ( Paenibacillus azoreducens ), Paenibacillus chibensis , Paenibacillus lentimorbus , Paenibacillus macerans , Paenibacillus polymyxa , Pantoea genus. S32 ( Pantoea sp. S32 ), Pediococcus Serevisiae ( Pediococcus cerevisiae ), Pediococcus halophilus ( Pediococcus halophilus ), Pedobactor ginseng ginsengisoli ( Pedobacter ginsengisoli ), Photorhabdus luminescens ( Photorhabdus luminescens ) . Sys ( Pseudomonas _ _ panipatensis ), Pseudomonas putida , Pseudomonas striata, Pseudomonas striata, Rhizopus delma , Rhizopus japonicus, Rhizopus oryzae, Tetrathiobacter kash One selected from the group consisting of Tetrathiobacter kashmirensis , Trichoderma hamatum , Trichoderma harzianum , and Variovorax boronicumulans ,
The second microorganism is Bacillus amyloliquefaciens , Bacillus brevis, Bacillus cereus , Bacillus circulans , Bacillus edaphicus , Bacillus Bacillus glucanolyticus, Bacillus licheniformis , Bacillus macerans , Bacillus mesonae , Bacillus mojavensis , Bacillus Musillajinosus ( Bacillus mucilaginosus ), Bacillus natto ( Bacillus natto ), Bacillus polymyxa ( Bacillus polymyxa ), Bacillus pumilus ( Bacillus pumilus ), Bacillus subtilus ( Bacillus subtilis ), Bacillus bilis mortis ( Bacillus vallismortis ), Bacillus bellegen Cis ( Bacillus velezensis ), Bacillus megaterium ( Bacllius megaterium ), Bacillus macroides ( Bcillus macroides ), Streptomyces asoensis ( Streptomyces asoensis ), Streptomyces carpinensis ( Streptomyces carpinensis ), Streptomyces pradiae ( Streptomyces fradiae ), Streptomyces griseochromogenesu ( Streptomyces griseochromogenes ), Streptomyces griseus ( Streptomyces griseus ), Streptomyces halstedii ( Streptomyces halstedii ), Streptomyces niger ( Streptomyces niger ), streptomyces Myces violaceus niger ( Streptomyces violaceusniger ), Rhodobacter azotoformans ( Rhodobacter azotoformans ), Rhodobacter capsulata ( Rhodobacter capsulata ), Rhodobacter capsulatus ( Rhodobacter capsulatus ), Rhodobacter rubrum ( Rhodobacter rubrum ), Rhodobacter sphaeroides ( Rhodobacter sphaeroides ), Rhodopseudomonas capusurata ( Rhodopseudomonas capusulata ), Rhodopseudomonas palustris ( Rhodopseudomonas palustris ), Rhodopseudomonas sphaeroides ( Rhodopseudomonas sphaeroides ), Rhodopseudomonas viridis ( Rhodopseudomonas viri dis ), sacaro Saccharomyces anamensis , Saccharomyces carlsbergensis, Saccharomyces cerevisiae , Saccharomyces sake , Pichia anomala ) And Pichia desticola ( Pichia deserticola ), Pichia stipitis ( Pichia stipitis ) Is one selected from the group consisting of,
The third microorganism is Acetobacter diazotrophicus, Acetobacter peroxydans, Acinetobacter calcoaceticus , Lactobacillus acidophilus, Lactobacillus acidophilus Bacillus bulgaricus, Lactobacillus casei, Lactobacillus casei subsp rhamnosus, Lactobacillus confusa , Lactobacillus crispatus , Lactobacillus Lactobacillus curvatus , Lactobacillus delbrueckii , Lactobacillus fermentum , Lactobacillus helveticus, Lactobacillus paracasei , Lactobacillus ferolens ( Lactobacillus perolens ), Lactobacillus plantarum ( Lactobacillus plantarum ), Lactobacillus rhamnosus ( Lactobacillus rhamnosus ), Lactobacillus reuteri ( Lactobacillus Reuteri ), Lactobacillus salivarius ( Lactobacillus salivarius ), Lactococcus lactis ( Lactococcus lactis ), Stefano Stephanoascus ciferrii , Streptococcus cremoris , Streptococcus lactis , Streptococcus thermophilus , Aspergillus niger , Aspergyl Loose oryzae ( Aspergillus oryzae ), Candida kefir ( Candida kefir ), Candida utilis ( Candida utilis ) and Candida valida ( Candida valida ) Utilization of lung culture medium discarded after culturing microorganisms, characterized in that one selected from the group consisting of A method for producing a microbial metabolite fertilizer.
The method of claim 1 or 2, wherein the first macroelement mixture is composed of a mixture of 40 to 90% by weight of the first metabolite and 10 to 60% by weight of the first macroelement,
The second major element mixture is formed by mixing 50 to 95% by weight of the second metabolite and 5 to 50% by weight of the second major element,
The trace element mixture is a method for producing a microbial metabolite fertilizer using waste culture medium discarded after culturing microorganisms, characterized in that the mixture is composed of a mixture of 50.0 to 99.999% by weight of the third metabolite and 0.0001 to 50% by weight of the trace element.
According to claim 3,
The step of forming the mixture of trace elements,
mixing the third metabolite and the trace element to form a first mixture;
adjusting the pH of the first mixture to 2.5 to 4.0 by adding 0.1 to 15 parts by weight of an organic acid based on 100 parts by weight of the first mixture;
A method for producing a microbial metabolite fertilizer using waste culture medium discarded after culturing microorganisms, comprising the step of chelating the pH-adjusted first mixture at 40-65 ° C. for 48-120 hours.
The method of claim 1, wherein the step of preparing the microbial metabolite fertilizer is a mixture of 70 to 90% by weight of the first macroelement mixture, 5 to 15% by weight of the second macroelement mixture, and 5 to 15% by weight of the trace element mixture. A method for producing a microbial metabolite fertilizer using waste culture medium discarded after culturing microorganisms, characterized in that being.
The method of claim 2, wherein the step of preparing the microbial metabolite fertilizer comprises 70 to 85 wt % of the first macro element mixture, 5 to 10 wt % of the second macro element mixture, and 5 to 10 wt % of the third macro element mixture. Method for producing a microbial metabolite fertilizer using waste culture medium discarded after cultivation of microorganisms, characterized in that% and 5 to 10% by weight of a mixture of trace elements are mixed.