CN112136738A - High-egg-holding-quantity red-swamp crayfish parent screening and breeding method - Google Patents
High-egg-holding-quantity red-swamp crayfish parent screening and breeding method Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
A method for screening and breeding high egg-carrying amount of red crayfish parents belongs to the technical field of screening of the red crayfish parents. The method comprises the following steps: (1) the method comprises the following steps of (1) initial screening of parents, (2) carrying out parent screening by using the width/full length of a first abdominal node as an index instead of an oviposition index, (3) intensive cultivation and ripening of the parents, (4) mating and breeding of the parents, (5) cultivation of ovigerous shrimps, (6) evaluation of ovigerous performance of the parents, (7) indoor cultivation of juvenile shrimps and (8) putting offspring seeds into a pond. The method indirectly selects the egg laying amount character of the red swamp crayfish by using the morphological character related to the egg laying amount as the breeding index, and solves the problems that the egg laying amount cannot be measured in advance, the egg-laying parent crayfish is not suitable for excessive operation, the egg-laying rate is low and the like in the actual production by adjusting the specification proportion of the male and female parent crayfish and the number of matched groups, provides a technical scheme for establishing a high-yield parent population of the red swamp crayfish, and lays a foundation for realizing the large-scale breeding of the red swamp crayfish.
Description
Technical Field
The invention belongs to the technical field of screening of the parents of the red crayfish, and particularly relates to a screening and breeding method of the parents of the red crayfish with high egg-carrying capacity.
Background
Red swamp crayfish (Cherax quadricarinatus) Belonging to the phylum Arthropoda, the phylum Crustacea, the class Hypocreales, the order Decoda, the order Abutilida, the family Procambaridae, the genus Metaplexis. The red swamp crayfish has strong adaptability, quick growth, no obvious feeding preference, large adult specification, long survival time after leaving water and convenient long-distance transportation. Has considerable market prospect. The introduction of red swamp crayfish from Hubei aquatic research institute as early as 1992 in China, and the first place is in the wide areaThe two provinces of east and Hubei successfully try to nourish. At present, the breeding industry of the red swamp crayfish in China is still in a primary stage, because the problems that fry is not easy to obtain and high-quality parents are lacked, the breeding of the red swamp crayfish is not effectively promoted all the time, and the large limitation exists, so that the breeding of the parent population of the red swamp crayfish with high egg yield has important promotion effects on realizing the genetic improvement of the red swamp crayfish and promoting the industrialized development of the red swamp crayfish.
The breeding habit of shrimp and crab animals is different from that of fish, and the parent shrimps are generally eliminated after breeding once, so that the egg holding amount of the parent shrimps cannot be measured in advance and then used as parents. In addition, the red swamp crayfish has the habit of laying eggs, and because the shrimp eggs are closely arranged, accurate measurement can not be carried out on the premise of not damaging the structure of the laid eggs, and because the male crayfish has no egg laying amount index, the male crayfish can not be effectively selected. In view of the above reasons, establishing a feasible screening technique for high egg-holding quantity of the parents of the red swamp crayfish is of great significance.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to design and provide a method for screening and breeding the high egg-carrying amount of the red crayfish parents. The method can quickly establish a stable parent group with high egg holding capacity by indirectly selecting the egg holding capacity index through morphological indexes before mating of male and female parent shrimps.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for screening and breeding high egg-carrying amount of red swamp crayfish parents is characterized by comprising the following steps:
(1) primary screening of parents: roughly selecting 80g or more female shrimps and 130g or more male shrimps in the red swamp crayfish, taking 1000-fold 2000 tails of the female shrimps and 300-fold 600 tails of the male shrimps with complete limbs and healthy body surface without damage, and placing the female shrimps and the male shrimps in an indoor cement pond for temporary culture;
(2) and (3) carrying out parent screening by taking the width/full length of the first abdominal node as an index to replace an oviposition index: randomly selecting 100 female and male shrimps temporarily bred in the step (1), accurately measuring the full length and the first abdominal node width, calculating a first abdominal node width/full length value, sorting the female and male shrimps from big to small according to the value, selecting and reserving female and male parents with the first abdominal node width/full length being arranged at the top 30%, and selecting and reserving all the parents by taking the 30 th data in the female and male parents as the screening indexes of the subsequent parents;
(3) intensive cultivation and ripening of parents: sterilizing the body surface of the parent selected in the step (2) by using 1mg/L povidone iodine solution, transferring the parent to a parent shrimp cultivation tank from the end of 10 months to the middle of 11 months, carrying out separate female and male cultivation at a density of 5 tails per square meter, carrying out reinforced cultivation, accelerating ripening after 2 months, gradually increasing the water temperature, increasing the temperature of 1 ℃ every day until the water temperature reaches 25 ℃, simultaneously increasing the bait feeding amount, and placing a concealed object in the parent shrimp cultivation tank;
(4) mating and breeding of parents: removing parent shrimps with broken limbs, injured body surface and poor vitality at the beginning of 3 months, selecting matched parent shrimps according to the standard that the specifications of male shrimps and female shrimps are 1.2:1, matching according to the proportion of 3 females to 1 male, placing in an indoor cement pond, and using 0.5m2The plastic drain baskets cover each group of parent shrimps to keep the indoor dark and quiet;
(5) culturing the egg-carrying shrimps: the egg-carrying condition of the female shrimps in the basket is checked regularly, the egg-carrying shrimps are transferred to an incubation pool, oxygenation and water body disinfection are enhanced, and the bait feeding amount is reduced;
(6) parent spawning performance evaluation: after all the young shrimps are separated from the parent, moving out the female shrimps, counting the number of the young shrimps, dividing by the number of the egg-carrying female shrimps, calculating the average egg-carrying amount of each female shrimp, comparing the average egg-carrying amount with the egg-carrying amount of the non-bred parent, and evaluating the parent selection effect;
(7) indoor culture of juvenile shrimps: using fine air stone to largely oxygenate, feeding live artemia and small particle compound feed, arranging a porous masking body and attachments after the shells of the young shrimps are removed, supplementing high-calcium feed, feeding the compound feed once in the morning, in the middle and at night from 8 days, and adjusting the illumination according to the brightness of 12h and the darkness of 12 h;
(8) and (3) putting seedlings into a pond: and (3) starting to put the young shrimps at the bottom of 4 months and at the beginning of 5 months, disinfecting the water body by using bromochlorohydantoin 5 days before putting the young shrimps, selecting young shrimps with the specification of 1.0cm, complete limbs, strong and healthy constitution, no damage, sensitive reaction and strong activity to put the young shrimps into the pond, feeding the young shrimps with the putting density of 75000 tails/hectare, starting to feed the young shrimps on the same day, starting an aerator at noon every day for 2 hours, and removing the whole pond of the purse net for putting the young shrimps into the pond after the young shrimps reach 2-3cm after 25-30 days.
The method for screening and breeding the high egg-carrying amount of the red swamp crayfish parents is characterized in that the intensive cultivation in the step (3) specifically comprises the following steps: besides the daily feed, animal feed is added, plant feed is added, and the water temperature of the hatching pond is kept at 18 ℃.
The method for screening and breeding the high egg-carrying amount of the red swamp crayfish parents is characterized in that the regular inspection in the step (5) specifically comprises the following steps: gently take up the female shrimp, grasp the skull with thumb and forefinger, firmly press the caudal ganglion with the other three fingers, and observe the egg-holding condition.
The method for screening and breeding the high egg-carrying amount of the red swamp crayfish parents is characterized in that the seedling releasing in the step (8) is specifically as follows: the method comprises the steps of putting the shrimp seedlings in the morning or evening of sunny weather, putting the shrimp seedlings in a cultivation pool for soaking bags and adjusting the temperature, wherein the temperature difference of water is not more than 2 ℃.
The method for screening and breeding the high egg-carrying amount of the red swamp crayfish parents is characterized in that the feeding in the step (8) is specifically as follows: feeding the feed 0 of the compound feed for the prawns for 1 time every evening, wherein the feeding coefficient is 15% of the weight of the prawns, feeding the crushed feed 1 of the compound feed for the prawns after 15 days, the feeding coefficient is 10%, feeding the feed 1 of the compound feed for the prawns after 25 days, the feeding coefficient is 5%, feeding the feeds 2 and 3 of the compound feed for the prawns after 2 months, the feeding coefficient is 3% before 7cm of the prawns, and the feeding coefficient is 1% after the prawns reach 10 cm.
The compound feed for the penaeus vannamei boone in the application is the existing feed, in particular to the compound feed for the penaeus vannamei boone, wherein No. 0, No. 1, No. 2 and No. 3 are conventional names for classifying the feed particle sizes in the field.
According to the method, the small sample population is measured, the breeding standard is determined, the specifications of the parent shrimps are rapidly and effectively screened, the morphological characters related to the egg laying amount are used as breeding indexes, the egg laying amount characters of the red swamp shrimps are indirectly selected, the difficulties that the egg laying amount cannot be measured in advance, the egg-carrying parent shrimps are not suitable for excessive operation, the egg-carrying rate is low and the like in actual production are solved by adjusting the specification proportion and the group number of the male and female parent shrimps, a technical scheme is provided for establishing the high-yield parent population of the red swamp shrimps, and a foundation is laid for realizing large-scale breeding of the red swamp shrimps.
Detailed Description
The present invention will be further illustrated by the following specific examples.
Example 1:
1. screening parents:
primary screening of parents: the female shrimps reach more than 80g and the male shrimps reach more than 130g according to the parent specification, rough selection is carried out, 2000 tails of the female shrimps with complete limbs and healthy body surfaces without damage and 600 tails of the male shrimps are taken as candidate parent shrimps and temporarily cultured in an indoor cement pond.
Parent screening was performed using the first abdominal node width/full length index instead of the oviposition index: first, 100 each of the temporarily-cultured female and male crayfishes are randomly selected, and the total length and the first abdominal section width are accurately measured by using a vernier caliper. Calculating a first abdominal width/full length value of the parent shrimps, sorting the data from big to small according to male and female, selecting and reserving a female parent and a male parent with the first abdominal width/full length being arranged at the top 30%, respectively taking the data of 30 th name in the female parent and the male parent as the screening index of the subsequent parent, and selecting and reserving the parent shrimps reaching the index in the subsequent measurement as the first generation parent.
Intensive cultivation and ripening of parents: and (3) disinfecting the body surface of the selected parent shrimps by using 1mg/L povidone iodine solution, transferring the parent shrimps to a parent shrimp cultivation tank in the late 10-11 middle ten days, and separately stocking male and female shrimps according to the density of 5 tails/square meter. The parent shrimp is intensively cultivated, animal baits such as fish, snail and the like are added in addition to the daily feed, and plant baits are matched. The bait source is strictly controlled to prevent the introduction of germs. In order to promote the parent shrimps to lay eggs synchronously, the water temperature in the hatching pond is kept at 18 ℃, the parent shrimps die due to too low temperature, and the ovaries of the female shrimps are not developed synchronously due to too high temperature. Ripening is started in 2 months every year, the water temperature is gradually increased, the water temperature is increased by 1 ℃ every day until the water temperature is increased to 25 ℃, and the bait feeding amount is increased, so that the synchronous development of the ovaries of female shrimps can be promoted, the female shrimps can shell once in the ovary development process, and therefore concealed objects such as tiles and the like need to be placed in the culture pond, and the protection of the soft-shell shrimps is realized.
2. Mating and breeding parent shrimps:
the proportion of the male and female parent shrimp matching specification and the number: and (5) carrying out male and female matching on the cultured candidate parent shrimps at the beginning of 3 months. Removing parent shrimps with broken limbs, injured body surfaces and poor vitality according to the specifications of male shrimps: female shrimp size = 1.2:1, selecting matched parent shrimps, and matching the parent shrimps according to the ratio of 3 females to 1 male in each group. After grouping, use 0.5m in an indoor cement pit2The plastic leaking basket covers each group of parent shrimps, can limit the moving range of each group of parent shrimps, but cannot obstruct water body communication to improve the egg-holding ratio of female shrimps. Compared with the traditional group mating, the method can obviously improve the egg-laying rate of the female shrimps, can enable the egg-laying time of the female shrimps to be as close as possible, enables the hatching time of the fries to be consistent, and is convenient for the fries to be taken out of the pond. Meanwhile, the operation can delay the speed of reducing the genetic diversity of the offspring, and greatly avoids suburb recession. After grouping, dim light is kept indoors, so that the disturbance is avoided as much as possible, and the natural mating is realized.
Culturing the egg-carrying shrimps: the oozing condition of female shrimps in the leaking frame is regularly checked, the female shrimps begin to lay eggs 24h after mating, the female shrimps after laying eggs roll the abdominal joints to protect fertilized eggs, the female shrimps need to be taken up lightly when observing the fertilized eggs and the tail joints are pressed, and the female shrimps are prevented from bouncing the tail joints to cause the fertilized eggs to fall off when struggling. The oogenesis shrimp is transferred to the hatching pond, and the demand of oogenesis shrimp to dissolved oxygen is higher, needs the reinforcing to oxygenate to strengthen the disinfection to the water, prevent that the embryonated egg from infecting the mould, oogenesis shrimp food intake reduces, stops even ingesting, should reduce the bait casting volume, avoids the quality of water that the incomplete bait too much arouses to worsen.
3. And (3) breeding of juvenile shrimps:
evaluation of parent ovum-holding performance: fertilized eggs develop to 42-45 days to form young shrimps, and the young shrimps are separated from the parent body completely and need about 3 days. And after all the young shrimps are separated from the parent, moving out the female shrimps. And counting the number of the young shrimps, dividing the number by the number of the egg-carrying female shrimps, and calculating the average egg-carrying amount of each female shrimp. And comparing the brood volume with the brood volume of the non-bred parents to evaluate the parent selection effect.
Indoor culture of juvenile shrimps: the oxygen consumption of the young shrimps is high, and the oxygen should be greatly oxygenated by using fine air stone. The young shrimps are separated from the parent and then start to eat, live artemia larvae are fed, and small-particle compound feed is supplemented. The larvae of the shrimps with eggs are dead due to incomplete molting caused by poor physique, insufficient feed in the early stage of the larvae, lack of calcium in feed and the like. The method is characterized in that young crayfish of the red swamp crayfish are hulled once 1-3 days after being separated from a parent, the hulling period is gradually prolonged along with the growth of the crayfish, the crayfish is fragile in the hulling process, a porous shelter and an accessory crop are arranged, meanwhile, high-calcium feed is supplemented, the young crayfish can eat a vacant shell after being hulled, the behavior is favorable for energy supplement, and the soft-shell crayfish which cannot eat the vacant shell is prone to death. The young shrimps are hatched for 8 days and fed with the compound feed once in the morning, in the middle and at night. The illumination is adjusted according to the day and night rhythm of 12h illumination and 12h darkness, and if the light of the culture environment is too strong, the color of the shrimp shell becomes light and even white, which is not beneficial to hiding.
And (3) putting seedlings into a pond: and (4) starting seedling release at the bottom of 4 months and at the beginning of 5 months, and disinfecting the water body by using bromochlorohydantoin 5 days before the seedling release. Selecting shrimp seeds with the specification of about 1.0cm, complete limbs, strong physique, no injury, sensitive reaction and lively and powerful for stocking, wherein the stocking density is 75000 tails per hectare. After 25 to 30 days, the shrimp seedlings are cultivated to 2cm to 3cm, and the purse nets are removed for stocking in the whole pool. It should be noted that the young shrimp is thrown in the morning or evening in sunny weather to avoid direct irradiation of sunlight, and the young shrimp is transported to the cultivation pool to be soaked in the bag for temperature adjustment to avoid the stress reaction of the young shrimp caused by the difference of water temperature over 2 ℃. When seedlings are put, the oxygen increasing pump is started to increase oxygen, and after seedlings are put, the oxygen increasing machine is started for 2 hours every noon. Feeding is started on the day of feeding, feed 0 of the compound feed for the Tongwei penaeus vannamei is used, feeding is carried out for 1 time every evening, the feeding coefficient is 15% of the weight of the penaeus vannamei, after 15 days, feed 1 of the compound feed for the Tongwei penaeus vannamei is fed, the feeding coefficient is 10%, after 25 days, feed 1 of the compound feed for the Tongwei penaeus vannamei is fed, the feeding coefficient is 5%, after 2 months, feed 2 and feed 3 of the compound feed for the Tongwei penaeus vannamei are fed, the feeding coefficient is 3% before 7cm of the penaeus vannamei, and the feeding coefficient is 1% after 10cm of the penaeus vannamei.
Example 2:
according to the method of the embodiment 1, after candidate parents of the red swamp crayfish arrive at a workshop, 1000 tails of female crayfish and 300 tails of male crayfish which are healthy and have no damage on body surface and large specifications are screened, then the parents are evaluated according to a first abdominal width/full length index, the data of 30 percent is taken as a screening standard, the screening standard of the female crayfish is made to be that the first abdominal width/full length is more than or equal to 0.216, the screening standard of the male crayfish is that the first abdominal width/full length is more than or equal to 0.203, 60 male crayfish and 180 female crayfish are screened out as parents, 60 male crayfish and 180 male crayfish are randomly selected as a control group, and the first abdominal width/full length ratio data of parent crayfish of the experimental group and the control group are shown in a. The male and female parts are matched according to the ratio of 3:1, and 60 groups are separated by using a plastic leakage basket to breed and wait for spawning. Meanwhile, the unselected control group female and male parent shrimps were treated according to the ratio of 3:1 group, and breeding was performed under the same conditions as those of the experimental group.
TABLE 1 first abdominal node Width/full Length comparison of the high producing parents of Red crayfish to the control group
Finally, 150 tail egg-carrying shrimps are obtained by the high-yield experimental group, the egg-carrying rate is 83%, 120 tail egg-carrying shrimps are obtained by the control group, the egg-carrying rate is 67%, and the egg-carrying rate of the high-yield experimental group is remarkably improved compared with that of the control group. Through statistics, 3.5 million shrimp fries are obtained in the high-yield experimental group, the average egg holding amount is 233 female shrimps per tail, 2 million shrimps are produced in the control group, and the average egg holding amount is 167 female shrimps per tail. The egg holding amount of the female shrimps in the high-yield experimental group is obviously higher than that of the control group, which shows that the method for indirectly selecting the egg holding amount by using the first abdominal node width/full length index is practical and effective.
After the juvenile shrimps are taken out of the pond for 3 months, 200 tails of the juvenile shrimps in the high egg-carrying group are randomly captured, 200 tails of the juvenile shrimps in the control group are accurately measured by using a vernier caliper, and after comparison, the first abdominal node width/full length of the juvenile shrimps in the high egg-carrying group is obviously higher than that of the control group (shown in table 2), so that the first abdominal node width index/full length of offspring is obviously selected, and the index is proved to be closely related to the egg-carrying quantity.
TABLE 2 first abdominal fat/full length comparison of the first generation of red crayfish
Claims (5)
1. A method for screening and breeding high egg-carrying amount of red swamp crayfish parents is characterized by comprising the following steps:
(1) primary screening of parents: roughly selecting 80g or more female shrimps and 130g or more male shrimps in the red swamp crayfish, taking 1000-fold 2000 tails of the female shrimps and 300-fold 600 tails of the male shrimps with complete limbs and healthy body surface without damage, and placing the female shrimps and the male shrimps in an indoor cement pond for temporary culture;
(2) and (3) carrying out parent screening by taking the width/full length of the first abdominal node as an index to replace an oviposition index: randomly selecting 100 female and male shrimps temporarily bred in the step (1), accurately measuring the full length and the first abdominal node width, calculating a first abdominal node width/full length value, sorting the female and male shrimps from big to small according to the value, selecting and reserving female and male parents with the first abdominal node width/full length being arranged at the top 30%, and selecting and reserving all the parents by taking the 30 th data in the female and male parents as the screening indexes of the subsequent parents;
(3) intensive cultivation and ripening of parents: sterilizing the body surface of the parent selected in the step (2) by using 1mg/L povidone iodine solution, transferring the parent to a parent shrimp cultivation tank from the end of 10 months to the middle of 11 months, carrying out separate female and male cultivation at a density of 5 tails per square meter, carrying out reinforced cultivation, accelerating ripening after 2 months, gradually increasing the water temperature, increasing the temperature of 1 ℃ every day until the water temperature reaches 25 ℃, simultaneously increasing the bait feeding amount, and placing a concealed object in the parent shrimp cultivation tank;
(4) mating and breeding of parents: removing parent shrimps with broken limbs, injured body surface and poor vitality at the beginning of 3 months, selecting matched parent shrimps according to the standard that the specifications of male shrimps and female shrimps are 1.2:1, matching according to the proportion of 3 females to 1 male, placing in an indoor cement pond, and using 0.5m2The plastic drain baskets cover each group of parent shrimps to keep the indoor dark and quiet;
(5) culturing the egg-carrying shrimps: the egg-carrying condition of the female shrimps in the basket is checked regularly, the egg-carrying shrimps are transferred to an incubation pool, oxygenation and water body disinfection are enhanced, and the bait feeding amount is reduced;
(6) parent spawning performance evaluation: after all the young shrimps are separated from the parent, moving out the female shrimps, counting the number of the young shrimps, dividing by the number of the egg-carrying female shrimps, calculating the average egg-carrying amount of each female shrimp, comparing the average egg-carrying amount with the egg-carrying amount of the non-bred parent, and evaluating the parent selection effect;
(7) indoor culture of juvenile shrimps: using fine air stone to largely oxygenate, feeding live artemia and small particle compound feed, arranging a porous masking body and attachments after the shells of the young shrimps are removed, supplementing high-calcium feed, feeding the compound feed once in the morning, in the middle and at night from 8 days, and adjusting the illumination according to the brightness of 12h and the darkness of 12 h;
(8) and (3) putting seedlings into a pond: and (3) starting to put the young shrimps at the bottom of 4 months and at the beginning of 5 months, disinfecting the water body by using bromochlorohydantoin 5 days before putting the young shrimps, selecting young shrimps with the specification of 1.0cm, complete limbs, strong and healthy constitution, no damage, sensitive reaction and strong activity to put the young shrimps into the pond, feeding the young shrimps with the putting density of 75000 tails/hectare, starting to feed the young shrimps on the same day, starting an aerator at noon every day for 2 hours, and removing the whole pond of the purse net for putting the young shrimps into the pond after the young shrimps reach 2-3cm after 25-30 days.
2. The method for screening and breeding the high egg-carrying capacity crayfish parents as claimed in claim 1, wherein the intensive cultivation in the step (3) is specifically as follows: besides the daily feed, animal feed is added, plant feed is added, and the water temperature of the hatching pond is kept at 18 ℃.
3. The method for screening and breeding the high egg-holding quantity of the parents of the red crayfish as claimed in claim 1, wherein the regular examination in the step (5) is specifically as follows: gently take up the female shrimp, grasp the skull with thumb and forefinger, firmly press the caudal ganglion with the other three fingers, and observe the egg-holding condition.
4. The method for screening and breeding the high egg-carrying capacity crayfish parents as claimed in claim 1, wherein the step (8) of releasing the fry is specifically as follows: the method comprises the steps of putting the shrimp seedlings in the morning or evening of sunny weather, putting the shrimp seedlings in a cultivation pool for soaking bags and adjusting the temperature, wherein the temperature difference of water is not more than 2 ℃.
5. The method for screening and breeding the high egg-carrying capacity crayfish parents as claimed in claim 1, wherein the feeding in the step (8) is specifically as follows: feeding the feed 0 of the compound feed for the prawns for 1 time every evening, wherein the feeding coefficient is 15% of the weight of the prawns, feeding the crushed feed 1 of the compound feed for the prawns after 15 days, the feeding coefficient is 10%, feeding the feed 1 of the compound feed for the prawns after 25 days, the feeding coefficient is 5%, feeding the feeds 2 and 3 of the compound feed for the prawns after 2 months, the feeding coefficient is 3% before 7cm of the prawns, and the feeding coefficient is 1% after the prawns reach 10 cm.
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