CN112119698A - Low-temperature storage method of corn seeds - Google Patents
Low-temperature storage method of corn seeds Download PDFInfo
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- CN112119698A CN112119698A CN202011085229.1A CN202011085229A CN112119698A CN 112119698 A CN112119698 A CN 112119698A CN 202011085229 A CN202011085229 A CN 202011085229A CN 112119698 A CN112119698 A CN 112119698A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01F—PROCESSING OF HARVESTED PRODUCE; HAY OR STRAW PRESSES; DEVICES FOR STORING AGRICULTURAL OR HORTICULTURAL PRODUCE
- A01F25/00—Storing agricultural or horticultural produce; Hanging-up harvested fruit
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
- A01N37/04—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof polybasic
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/10—Apiaceae or Umbelliferae [Carrot family], e.g. parsley, caraway, dill, lovage, fennel or snakebed
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- Agronomy & Crop Science (AREA)
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Abstract
The invention discloses a low-temperature storage method of corn seeds, belonging to the technical field of seed storage; the low-temperature storage method of the corn seeds is realized by three steps of seed treatment, ultrasonic seed dressing and dry storage. According to the invention, the angelica powder, the codonopsis pilosula powder and the cobalt oxalate powder are subjected to pulse electromagnetic treatment, so that the influence of diseases on crops can be effectively reduced, the cold damage resistance of seeds is improved, the moisture content is reduced, the absorption is accelerated during later treatment, the seed activity is improved, and the storage period is prolonged; according to the invention, the angelica powder and the codonopsis pilosula powder are subjected to secondary treatment to form a shell for protecting seeds, so that certain obstruction on the seeds is avoided, the seeds can grow healthily in a adversity environment, and the crop yield is increased; the method for storing the corn seeds has the advantages that the germination rate of the seeds is 92.4-94.1%, the disease incidence rate is greatly reduced, the yield per mu is 614.7-616.6 kg/mu, the yield is increased by 22.77-23.17%, and the economic benefit is remarkable.
Description
Technical Field
The invention belongs to the technical field of seed storage, and particularly relates to a low-temperature storage method of corn seeds.
Background
Corn (Zea mays L.) is an annual herb of the family gramineae, is an important feed source for the animal husbandry, the aquaculture industry and the like as a high-yield food crop in china, and is also one of indispensable raw materials for food, medical health, light industry, chemical industry and the like. Because the corn resources are extremely rich, cheap and easy to obtain, the corn also has a plurality of biological activities, such as oxidation resistance, tumor resistance, blood sugar reduction, immunity improvement, bacteriostasis, sterilization and the like, and has wide development and application prospects.
Due to the difference of the maturity of the seeds, the crushing during threshing is increased, and the physiological activity of the seeds can be increased under the influence of various factors such as heat injury or freeze injury. Promoting the enhancement of respiration and preventing the seeds from being stored easily. When the seeds are damaged by heat, the seed pile is mildewed due to the rise of temperature; the most easily occurring problem of seeds under cold damage is that the seeds are difficult to fall water due to early attack of cold tide. Once suffering from freezing damage, the germination rate decreases significantly in the next year. Currently, the corn seeds mainly have the influence of heat injury and cold injury in the storage process.
Through the search of the existing documents, the patent application of Chinese patent publication No. CN10625956A, published 2017, 1 month and 4 days, discloses a seed treatment agent for storing corn seeds, which can effectively prevent the seeds from mildewing and rancidity and prolong the storage period of the corn seeds, but the seeds have insufficient storage activity and insufficient viability in adverse environments, and the yield and diseases of the seeds can not meet the requirements of farmers when cold damage occurs.
Disclosure of Invention
The invention aims to provide a low-temperature storage method of corn seeds, which can prolong the corn seeds, improve the seed vitality, and obviously improve the crop yield and reduce the plant diseases and insect pests in the cold weather.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for cryopreservation of corn seeds comprises the following steps:
1) seed treatment: eliminating necrotic or germinated but broken seeds, contacting the seeds with anhydrous light alcohol and catalyst (Fe)2O3-CaO-SiO2And B2O3Is prepared according to a certain mass ratio, wherein B2O3The mass ratio of (A) is Fe2O3-CaO-SiO25% to 10% ofThen carrying out ventilation and disinfection treatment and then carrying out liquid spraying treatment by using clear water;
2) ultrasonic seed dressing: selecting angelica powder, codonopsis pilosula powder and cobalt oxalate powder, uniformly mixing, uniformly stirring in a sodium carbonate solution in a water bath at 30-35 ℃, then treating a reaction system in a low-frequency pulse magnetic field, simultaneously performing low-pressure electromagnetic treatment, freezing at-28-26 ℃ for 3-6 hours after the treatment is finished, filtering to obtain frozen corn seeds, soaking the frozen corn seeds in a seed-dressing agent solution with the mass fraction of 4.0-5.2%, and performing ultrasonic treatment at 35-40 kHz for 30-55 minutes to obtain ultrasonic corn seeds;
3) drying and storing: selecting the angelica sinensis powder and the codonopsis pilosula powder in the step 2), adding a small amount of corn flour, adding water, mixing uniformly, making into a hollowed shell, freezing, standing corn seeds in the sun for 6-10 min, storing in the hollowed shell, and ventilating to obtain the stored seeds.
Further, the mass ratio of the anhydrous light alcohol to the catalyst in the step 1) is 20-26: 30-34.
Further, the weight parts of the angelica powder, the codonopsis pilosula powder and the cobalt oxalate powder in the step 2) are respectively 30-36 parts of the angelica powder, 40-45 parts of the codonopsis pilosula powder and 15-25 parts of the cobalt oxalate powder.
Further, the pressure of the low-pressure electromagnetic treatment in the step 2) is 1.2-1.4 MPa.
Furthermore, the pulse time of the pulse magnetic field is 6-8 min, the pulse voltage is 400-600V, and the pulse frequency is 6-10 Hz.
Compared with the prior art, the invention has the beneficial effects that:
1) when the method is used for treating seeds, the anhydrous light alcohol and the catalyst are used for combined treatment, so that the emergence rate and the germination rate of the seeds after planting can be improved.
2) According to the invention, the angelica powder, the codonopsis pilosula powder and the cobalt oxalate powder are subjected to pulse electromagnetic treatment, so that the influence of diseases on crops can be effectively reduced, the cold damage resistance of seeds is improved, the moisture content is reduced, the absorption is accelerated during later treatment, the seed activity is improved, and the storage period is prolonged.
3) According to the invention, the angelica powder and the codonopsis pilosula powder are subjected to secondary treatment to form a shell for protecting seeds, so that certain obstruction on the seeds is avoided, the seeds can grow healthily in a adversity environment, and the crop yield is increased.
4) The method for storing the corn seeds has the advantages that the germination rate of the seeds is 92.4-94.1%, the storage life reaches 6 years and 4 months, the disease incidence rate is greatly reduced, the emergence rate is 93.6-94.1%, the yield per mu is 614.7-616.6 kg/mu, the yield is increased by 22.77-23.17%, and the economic benefit is remarkable.
Detailed Description
The following examples further describe in detail specific embodiments of the present invention. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1:
a method for cryopreservation of corn seeds comprises the following steps:
1) seed treatment: eliminating necrotic or germinated but broken seeds, contacting the seeds with anhydrous light alcohol and catalyst (Fe)2O3-CaO-SiO2And B2O3Is prepared according to a certain mass ratio, wherein B2O3The mass ratio of (A) is Fe2O3-CaO-SiO25 percent of the total amount of the active ingredients, performing ventilation and disinfection treatment, and then performing spray treatment by using clear water; the mass ratio of the anhydrous light alcohol to the catalyst is 20: 30.
2) Ultrasonic seed dressing: selecting angelica powder, codonopsis pilosula powder and cobalt oxalate powder, uniformly mixing, uniformly stirring in a sodium carbonate solution in a water bath at 30 ℃, then treating a reaction system in a low-frequency pulse magnetic field, simultaneously performing low-pressure electromagnetic treatment, freezing at-28 ℃ for 3 hours after the treatment is finished, filtering to obtain frozen corn seeds, soaking the frozen corn seeds in a seed-dressing agent solution with the mass fraction of 4.0%, and performing ultrasonic treatment at 35kHz for 30 minutes to obtain ultrasonic corn seeds; the weight parts of the angelica powder, the codonopsis pilosula powder and the cobalt oxalate powder are respectively 30 parts of the angelica powder, 40 parts of the codonopsis pilosula powder and 15 parts of the cobalt oxalate powder. The pressure of the low-pressure electromagnetic treatment is 1.2 MPa. The pulse time of the pulse magnetic field is 6min, the pulse voltage is 400V, and the pulse frequency is 6 Hz.
3) Drying and storing: selecting the angelica sinensis powder and the codonopsis pilosula powder in the step 2), adding a small amount of corn flour, adding water, mixing uniformly, making into a hollowed shell, freezing, standing corn seeds in the sun for 6min, storing in the hollowed shell, and ventilating to obtain the stored seeds.
Example 2:
a method for cryopreservation of corn seeds comprises the following steps:
1) seed treatment: eliminating necrotic or germinated but broken seeds, contacting the seeds with anhydrous light alcohol and catalyst (Fe)2O3-CaO-SiO2And B2O3Is prepared according to a certain mass ratio, wherein B2O3The mass ratio of (A) is Fe2O3-CaO-SiO28 percent of the total amount of the active ingredients, performing ventilation and disinfection treatment, and then performing spray treatment by using clear water; the mass ratio of the anhydrous light alcohol to the catalyst is 23: 32.
2) Ultrasonic seed dressing: selecting angelica powder, codonopsis pilosula powder and cobalt oxalate powder, uniformly mixing, uniformly stirring in a sodium carbonate solution in a water bath at 32 ℃, then treating a reaction system in a low-frequency pulse magnetic field, simultaneously performing low-pressure electromagnetic treatment, after the treatment is finished, freezing at-27 ℃ for 4 hours, filtering to obtain frozen corn seeds, soaking the frozen corn seeds in a seed-dressing agent solution with the mass fraction of 4.6%, and performing ultrasonic treatment at 38kHz for 40 minutes to obtain ultrasonic corn seeds; the weight parts of the angelica powder, the codonopsis pilosula powder and the cobalt oxalate powder are 33 parts of the angelica powder, 42 parts of the codonopsis pilosula powder and 20 parts of the cobalt oxalate powder respectively. The pressure of the low-pressure electromagnetic treatment is 1.3 MPa. The pulse time of the pulse magnetic field is 7min, the pulse voltage is 500V, and the pulse frequency is 8 Hz.
3) Drying and storing: selecting the angelica sinensis powder and the codonopsis pilosula powder in the step 2), adding a small amount of corn flour, adding water, mixing uniformly, making into a hollowed shell, freezing, standing corn seeds in the sun for 8min, storing in the hollowed shell, and ventilating to obtain the stored seeds.
Example 3:
a method for cryopreservation of corn seeds comprises the following steps:
1) seed treatment: eliminating necrotic or germinated but broken seeds, contacting the seeds with anhydrous light alcohol and catalyst (Fe)2O3-CaO-SiO2And B2O3Is prepared according to a certain mass ratio, wherein B2O3The mass ratio of (A) is Fe2O3-CaO-SiO210 percent of the total amount of the active ingredients, performing ventilation and disinfection treatment, and then performing spray treatment by using clear water; the mass ratio of the anhydrous light alcohol to the catalyst is 26: 34.
2) Ultrasonic seed dressing: selecting angelica powder, codonopsis pilosula powder and cobalt oxalate powder, uniformly mixing, uniformly stirring in a sodium carbonate solution in a water bath at 35 ℃, then treating a reaction system in a low-frequency pulse magnetic field, simultaneously performing low-pressure electromagnetic treatment, after the treatment is finished, freezing at-26 ℃ for 6 hours, filtering to obtain frozen corn seeds, soaking the frozen corn seeds in a seed-dressing agent solution with the mass fraction of 5.2%, and performing ultrasonic treatment at 40kHz for 55 minutes to obtain ultrasonic corn seeds; the weight parts of the angelica powder, the codonopsis pilosula powder and the cobalt oxalate powder are 36 parts of the angelica powder, 45 parts of the codonopsis pilosula powder and 25 parts of the cobalt oxalate powder respectively. The pressure of the low-pressure electromagnetic treatment is 1.4 MPa. The pulse time of the pulse magnetic field is 8min, the pulse voltage is 600V, and the pulse frequency is 10 Hz.
3) Drying and storing: selecting the angelica sinensis powder and the codonopsis pilosula powder in the step 2), adding a small amount of corn flour, adding water, mixing uniformly, making into a hollowed shell, freezing, placing corn seeds in the sun for 10min, storing in the hollowed shell, and ventilating to obtain the stored seeds.
Examples of the experiments
After the corn seeds are stored according to the method of the invention, the yield of the seeds after storage and the influence of pests on the crops are shown in table 1, and the patent documents in the background art documents are selected as comparative examples.
As can be seen from Table 1, when the corn seeds are stored by the method, the germination rate of the seeds is 92.4-94.1%, the storage period reaches 6 years and 4 months, the disease incidence rate is greatly reduced, the emergence rate is 93.6-94.1%, the yield per mu is 614.7-616.6 kg/mu, the yield is increased by 22.77-23.17%, and the economic benefit is remarkable.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (5)
1. A method for cryopreservation of corn seeds is characterized by comprising the following steps:
1) seed treatment: eliminating necrotic or germinated but broken seeds, contacting the seeds with anhydrous light alcohol and catalyst (Fe)2O3-CaO-SiO2And B2O3Is prepared according to a certain mass ratio, wherein B2O3The mass ratio of (A) is Fe2O3-CaO-SiO25% -10% of the total amount of the raw materials, then performing ventilation sterilization treatment and then performing liquid spraying treatment by using clear water;
2) ultrasonic seed dressing: selecting angelica powder, codonopsis pilosula powder and cobalt oxalate powder, uniformly mixing, uniformly stirring in a sodium carbonate solution in a water bath at 30-35 ℃, then treating a reaction system in a low-frequency pulse magnetic field, simultaneously performing low-pressure electromagnetic treatment, freezing at-28-26 ℃ for 3-6 hours after the treatment is finished, filtering to obtain frozen corn seeds, soaking the frozen corn seeds in a seed-dressing agent solution with the mass fraction of 4.0-5.2%, and performing ultrasonic treatment at 35-40 kHz for 30-55 minutes to obtain ultrasonic corn seeds;
3) drying and storing: selecting the angelica sinensis powder and the codonopsis pilosula powder in the step 2), adding a small amount of corn flour, adding water, mixing uniformly, making into a hollowed shell, freezing, standing corn seeds in the sun for 6-10 min, storing in the hollowed shell, and ventilating to obtain the stored seeds.
2. The method for low-temperature storage of corn seeds as claimed in claim 1, wherein the mass ratio of the anhydrous light alcohol to the catalyst in the step 1) is 20-26: 30-34.
3. The method for low-temperature storage of corn seeds as claimed in claim 1, wherein the weight parts of the angelica powder, the codonopsis pilosula powder and the cobalt oxalate powder in the step 2) are 30-36 parts of the angelica powder, 40-45 parts of the codonopsis pilosula powder and 15-25 parts of the cobalt oxalate powder respectively.
4. The method for low-temperature storage of corn seeds as claimed in claim 1, wherein the pressure of the low-pressure electromagnetic treatment in step 2) is 1.2-1.4 MPa.
5. The method of claim 1, wherein the pulse time of the pulsed magnetic field is 6-8 min, the pulse voltage is 400-600V, and the pulse frequency is 6-10 Hz.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011085229.1A CN112119698A (en) | 2020-10-12 | 2020-10-12 | Low-temperature storage method of corn seeds |
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| CN202011085229.1A CN112119698A (en) | 2020-10-12 | 2020-10-12 | Low-temperature storage method of corn seeds |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114946316A (en) * | 2022-05-27 | 2022-08-30 | 云南省林业和草原科学院 | Storage method for improving germination rate of gramineous plant seeds |
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| CN1922995A (en) * | 2005-08-31 | 2007-03-07 | 青岛海联植物科技研究所 | Technique of improving maize cold resistant and moisture proof character by using cryoprotectant |
| JP2007077118A (en) * | 2005-09-16 | 2007-03-29 | Hyogo Prefecture | Antagonistic microorganism coating seed, method for producing the same, and method for controlling disease in crop |
| CN106717244A (en) * | 2016-11-15 | 2017-05-31 | 黄家七 | A kind of low-temperature storage method of corn seed |
| CN108739799A (en) * | 2018-06-15 | 2018-11-06 | 合肥扬扬农业科技有限公司 | A method of improving corn seed keeping quality |
| CN109451913A (en) * | 2018-12-13 | 2019-03-12 | 袁米农业科技有限公司 | A kind of storage method of corn seed |
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2020
- 2020-10-12 CN CN202011085229.1A patent/CN112119698A/en active Pending
Patent Citations (5)
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| CN1922995A (en) * | 2005-08-31 | 2007-03-07 | 青岛海联植物科技研究所 | Technique of improving maize cold resistant and moisture proof character by using cryoprotectant |
| JP2007077118A (en) * | 2005-09-16 | 2007-03-29 | Hyogo Prefecture | Antagonistic microorganism coating seed, method for producing the same, and method for controlling disease in crop |
| CN106717244A (en) * | 2016-11-15 | 2017-05-31 | 黄家七 | A kind of low-temperature storage method of corn seed |
| CN108739799A (en) * | 2018-06-15 | 2018-11-06 | 合肥扬扬农业科技有限公司 | A method of improving corn seed keeping quality |
| CN109451913A (en) * | 2018-12-13 | 2019-03-12 | 袁米农业科技有限公司 | A kind of storage method of corn seed |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114946316A (en) * | 2022-05-27 | 2022-08-30 | 云南省林业和草原科学院 | Storage method for improving germination rate of gramineous plant seeds |
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