CN112094355A - 一种用于临床诊断的复合质控品及其制备方法 - Google Patents
一种用于临床诊断的复合质控品及其制备方法 Download PDFInfo
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- CN112094355A CN112094355A CN202011316538.5A CN202011316538A CN112094355A CN 112094355 A CN112094355 A CN 112094355A CN 202011316538 A CN202011316538 A CN 202011316538A CN 112094355 A CN112094355 A CN 112094355A
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- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 23
- 239000003550 marker Substances 0.000 claims abstract description 20
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- 229920001184 polypeptide Polymers 0.000 claims abstract description 19
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- 238000008416 Ferritin Methods 0.000 claims abstract description 15
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Abstract
本发明公开了一种基于铁蛋白纳米颗粒载体连接的多个诊断标志物特异性表位多肽分子的复合质控品及其制备方法，通过抗体检测及表位分析技术我们可以将绝大多数的诊断标志物蛋白的优势表位定位出来，然后通过连接酯链接到铁蛋白分子上，形成“Fer‑Linker‑Epitope Peptide”复合物，将合成的该复合物基因序列通过特定的载体转入原核大肠杆菌重组表达，这样含有多个诊断标志物优势表位多肽分子的复合抗原（质控品）就可以同时被多个诊断试剂检测出来。本发明的质控品检测效率高，成本低，稳定性极佳，可在2～8℃条件下长期稳定保存。
Description
技术领域
本发明涉及免疫诊断试剂的质控品技术领域，具体为一种用于临床诊断的复合质控品及其制备方法。
背景技术
在我国，体外诊断试剂是指：可单独使用或与仪器、器具、设备或系统组合使用,在疾病的预防、诊断、治疗监测、预后观察、健康状态评价以及遗传性疾病的预测过程中,用于对人体样本(各种体液、细胞、组织样本等)进行体外检测的试剂、试剂盒、校准品(物)、质控品(物)等。
质控品是体外诊断试剂的重要而必不可少的组成部分，主要用于对体外诊断试剂进行质量控制。在实际的临床应用过程中，检验人员使用体外诊断试剂检测病人的样本（血清，尿液等），在此之前，需要对试剂进行质量验证以确保检测结果的准确性。
质控品是指经过一系列严格的量值溯源体系，有确定的靶值及允许的偏差范围的一类质量稳定的生物化学制品。体外诊断试剂在使用之前需要先测试配套的质控品，若结果显示在允许的偏差范围内则判定试剂质量合格，可用于检测病人样本并报告正确的结果；若结果显示超出了允许的偏差范围则判定试剂盒质量不合格，不可用于检测病人样本，否则会导致错误的诊断结果。
在免疫诊断试剂产品中，质控品通常是用抗原（靶蛋白）按照一定的流程配置的，并对其进行赋值和稳定性的验证。传统的质控品多为单一项目的质控品，且多使用靶蛋白分子序列全长作为配置质控品的原料，这样的制备方法效率低下，成本高，且通常稳定性较差（需要制成冻干品以提高稳定性）。
发明内容
本发明要解决的技术问题是克服现有技术中免疫诊断试剂的质控品为单一项目的质控品，制备效率低的缺陷，提供一种复合质控品。
为了解决上述技术问题，本发明提供了如下的技术方案：
一种基于铁蛋白纳米颗粒载体连接的多个诊断标志物特异性表位多肽分子的复合质控品及其制备方法，其基本原理如下：通过抗体检测及表位分析技术我们可以将绝大多数的诊断标志物蛋白的优势表位（一个表位通常是由5~7个氨基酸组成的多肽）定位出来，然后通过连接酯（Linker）链接到铁蛋白（Fer）分子上，形成“Fer-Linker-Epitope Peptide”复合物，并合成的该复合物基因序列通过特定的载体转入原核大肠杆菌重组表达，这样含有多个诊断标志物优势表位多肽分子的复合抗原（质控品）就可以同时被多个诊断试剂检测出来。
本发明的质控品检测效率高，成本低，稳定性极佳，可在2～8℃条件下长期稳定保存。
通常，这里的Linker是指GGG3（谷氨酸-谷氨酸-谷氨酸-丝氨酸），Peptide是指经过表位分析鉴定方法测出的一段5~7个氨基酸多肽，如PCT（降钙素原）蛋白序列如下，其序列如SEQ ID NO.13所示：
APFRSALESSPADPATLSEDEARLLLAALVQDYVQMKASELEQEQEREGSSLDSPRS-KR-CGNLSTCMLGTYTQDFNKFHTFPQTAIGVGAP-GKKR-DMSSDLERDHRPHVSMPQNAN
鉴定的优势表位为：MKASEL。
该方法制备的复合质控品相比于传统的单一品种质控品有如下优势：
一、产品使用更加高效便捷：复合质控品含有多个诊断标志物的优势表位，可以实现一个质控品多个项目同时检测的高效率，在制备和使用两个过程中都避免了重复操作。
二、成本更低：一方面使用的原料仅为一个片段分子而非蛋白全长，节约了表达全长蛋白的成本；另一方面省去了冻干及重复制备的成本，极大降低了平均到单个质控品的制备成本。
三、稳定性极佳：由于铁蛋白分子量大，结构稳定（常被用着疫苗的连接载体），整个复合质控品分子展现了极佳的液体稳定性，可在2~8度条件下长期稳定保存。
附图说明
附图用来提供对本发明的进一步理解，并且构成说明书的一部分，与本发明的实施例一起用于解释本发明，并不构成对本发明的限制。在附图中：
图1是“Fer-Linker-Epitope Peptide”复合物的模型图。
具体实施方式
以下结合附图对本发明的优选实施例进行说明，应当理解，此处所描述的优选实施例仅用于说明和解释本发明，并不用于限定本发明。
实施例
一种用于临床诊断的复合质控品，包括铁蛋白和与铁蛋白通过连接酯链接的若干个诊断标志物特异性表位多肽分子。
铁蛋白是由24个单体自组装形成的球形聚体，是分子结构非常稳定纳米颗粒蛋白，其中C、N terminal和loop zone均可以用于插入外源基因片段，可将外源多肽或蛋白稳定的展示在球形颗粒的表面，基于上述原理，铁蛋白载体作为纳米颗粒疫苗载体，药物呈递，活体成像等领域被广泛关注。本研究中我们利用分子生物学方法将多诊断标志物的优势表位序列在基因层面上串联到铁蛋白上，利用原核大肠杆菌表达系统表达出含有多诊断标志位优势表位的铁蛋白复合质控品。
具体制备方法：
铁蛋白载体氨基酸序列，其序列如SEQ ID NO.1所示。
MLSKDIIKLLNEQVNKEMNSSNLYMSMSSWCYTHSLDGAGLFLFDHAAEEYEHAKKLIIFLNENNVPVQLTSISAPEHKFEGLTQIFQKAYEHEQHISESINNIVDHAIKSKDHATFNFLQWYVAEQHEEEVLFKDILDKIELIGNENHGLYLADQYVKGIAKSRKS
多诊断标志物预测的优势表位如下：
PCT：MKASEL，其序列如SEQ ID NO.2所示；
IL6：QNRFESSEEQ，其序列如SEQ ID NO.3所示；
Myo：ATKHKIPVK，其序列如SEQ ID NO.4所示。
CRP：APLTKPL，其序列如SEQ ID NO.5所示。
PGI：GSFLYYA，其序列如SEQ ID NO.6所示。
PGII；GTNFVY，其序列如SEQ ID NO.7所示。
BNP：CFGRKMDRI，其序列如SEQ ID NO.8所示。
SAA：ANYIGSDKY，其序列如SEQ ID NO.9所示。
将各诊断标志物优势表位序列串联插入到铁蛋白的序列中，插入后的序列如SEQID NO.10所示：
MHHHHHHGSMKASELGGGGSLSKDIIKLLNEQVNKEMNSSNLYMSMSSWCYTHGSQNRFESSEEGSSLDGAGLFLFDHAAEEYEHAKKLIIFLNENNGSATKHKIPVKGSAPLTKPLGSFLYYAVPVQLGSGTNFVYGSTSISAPEHKFEGLTQIFQKAYEHEQHISESINNIVDHAIKSGSGTNFVYGSCFGRKMDRIGSANYIGSDKYGSKDHATFNFLQWYVAEQHEEEVLFKDILDKIELIGNENHGLYLADQYVKGIAKSRKS
将上述序列用密码子优化软件在大肠杆菌表达系统中优化，优化后序列如SEQ IDNO.11所示：
CATATGCACCATCATCATCACCATGGTAGCATGAAAGCAAGCGAATTAGGTGGTGGTGGTAGCCTGAGCAAAGATATTATCAAACTGCTGAATGAACAGGTGAACAAAGAAATGAATAGCAGCAACCTGTATATGAGCATGAGCAGCTGGTGTTATACCCATGGTAGTCAGAATCGTTTTGAAAGCAGTGAAGAGGGTAGCAGCCTGGATGGTGCAGGTCTGTTTCTGTTTGATCATGCAGCCGAAGAATATGAGCACGCAAAAAAACTGATCATCTTCCTGAATGAAAATAATGGCAGCGCCACCAAACATAAAATTCCGGTTAAAGGTAGCGCACCGCTGACCAAACCGCTGGGTAGCTTTCTGTATTATGCAGTTCCGGTTCAGTTAGGTAGCGGCACCAATTTTGTTTATGGTAGCACCAGCATTAGTGCACCGGAACATAAATTTGAAGGTCTGACCCAGATTTTCCAGAAAGCCTATGAACATGAACAGCATATTAGCGAGAGCATCAACAACATTGTGGATCATGCAATTAAAAGCGGTTCAGGTACGAACTTTGTGTATGGTTCATGTTTTGGTCGTAAAATGGATCGTATTGGTAGCGCCAATTATATCGGCAGCGATAAATATGGCAGCAAAGATCATGCCACCTTTAACTTTCTGCAGTGGTATGTTGCAGAACAGCATGAAGAAGAAGTGCTGTTTAAAGACATCCTGGATAAAATTGAACTGATCGGCAATGAAAACCATGGTCTGTATCTGGCAGATCAGTATGTGAAAGGTATTGCCAAAAGCCGCAAAAGCTAACTCGAG
双下划线分别为NdeI和XhoI酶切位点；
将上述核苷酸序列送基因合成公司进行基因合成并用NdeI&XhoI酶切位点克隆至pET-30a(+)表达载体中,完成表达质粒的构建。
将构建好的质粒转化到BL21（DE3）感受态中进行诱导表达；当菌液OD600nm达到0.6时，加入终浓度为0.1mM的IPTG，16℃诱导表达24h，12000g，离心5min；用lysis buffer（50mM NaH2PO4,300mM NaCl，10mM imidazole, pH8.0）重悬菌体，超声破碎菌体后，12000g,离心30min，上清用0.45um滤器过滤；Lysis buffer平衡Ni NTA Beads 6FF后上样，Wash buffer（50mM NaH2PO4,300mM NaCl，60mM imidazole, pH8.0）洗涤20倍柱体积，Elution buffer（50mM NaH2PO4,300mM NaCl，250mM imidazole, pH8.0）洗脱目的蛋白，将蛋白透析至PBS缓冲液中保存即得该复合质控品蛋白。
将制备好的8种复合质控品进行靶值测定与稳定性验证（全部使用进口品牌且在国内已注册、临床使用的体外诊断试剂盒验证），数据如下：
表1实施例获得的质控品稳定性数据
表1显示了实施例所得的质控品的稳定性数据，由表1可见本发明的质控品37℃高温放置30天测试靶值结果无明显变化，稳定性极佳，在冷藏2～8℃条件下，放置380天靶值仍然在允许的偏差范围内。
备注：表1展示了8个诊断项目的复合质控品，但本发明包括但不限于这8项的复合质控品，本发明的质控项目包含目前临床上运用的所有免疫诊断试剂盒质控品（以国家食品药品监督管理局网站能够查询到的免疫诊断项目为准）。
质控品均一性验证
随机抽取同一生产批次的10瓶质控品，每包装单元测试1次，按下面的公式计算测试结果的平均值和标准差S1；另用上述质控品中的1瓶连续测试10次数，计算测试结果的平均值和标准差S2；按下列各公式计算瓶间重复性CV%。
公式 1 
公式 2 
公式 3 
公式 4 
当S1<S2时，令CV瓶间=0
式中：
----平均值；S----标准差；n----测量次数；xi----指定参数第i 次测量值。
结果如下：
表2 PCT质控品的均一性验证
表3 IL6质控品的均一性验证
表4Myo质控品的均一性验证
表5 BNP质控品的均一性验证
表6 PGI质控品的均一性验证
表7 PGII质控品的均一性验证
表8 SAA质控品的均一性验证
表9 CRP质控品的均一性验证
上述数据表明本发明中的复合质控品具有良好的瓶间重复性，各测试项目重复性CV小于1%。
最后应说明的是：以上所述仅为本发明的优选实施例而已，并不用于限制本发明，尽管参照前述实施例对本发明进行了详细的说明，对于本领域的技术人员来说，其依然可以对前述各实施例所记载的技术方案进行修改，或者对其中部分技术特征进行等同替换。凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。
序列表
<110> 南京佰抗生物科技有限公司
<120> 一种用于临床诊断的复合质控品及其制备方法
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Leu Gly Ser Gly Thr Asn Phe Val Tyr Gly Ser Thr Ser Ile Ser Ala
130 135 140
Pro Glu His Lys Phe Glu Gly Leu Thr Gln Ile Phe Gln Lys Ala Tyr
145 150 155 160
Glu His Glu Gln His Ile Ser Glu Ser Ile Asn Asn Ile Val Asp His
165 170 175
Ala Ile Lys Ser Gly Ser Gly Thr Asn Phe Val Tyr Gly Ser Cys Phe
180 185 190
Gly Arg Lys Met Asp Arg Ile Gly Ser Ala Asn Tyr Ile Gly Ser Asp
195 200 205
Lys Tyr Gly Ser Lys Asp His Ala Thr Phe Asn Phe Leu Gln Trp Tyr
210 215 220
Val Ala Glu Gln His Glu Glu Glu Val Leu Phe Lys Asp Ile Leu Asp
225 230 235 240
Lys Ile Glu Leu Ile Gly Asn Glu Asn His Gly Leu Tyr Leu Ala Asp
245 250 255
Gln Tyr Val Lys Gly Ile Ala Lys Ser Arg Lys Ser
260 265
<210> 11
<211> 816
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 11
Cys Ala Thr Ala Thr Gly Cys Ala Cys Cys Ala Thr Cys Ala Thr Cys
1 5 10 15
Ala Thr Cys Ala Cys Cys Ala Thr Gly Gly Thr Ala Gly Cys Ala Thr
20 25 30
Gly Ala Ala Ala Gly Cys Ala Ala Gly Cys Gly Ala Ala Thr Thr Ala
35 40 45
Gly Gly Thr Gly Gly Thr Gly Gly Thr Gly Gly Thr Ala Gly Cys Cys
50 55 60
Thr Gly Ala Gly Cys Ala Ala Ala Gly Ala Thr Ala Thr Thr Ala Thr
65 70 75 80
Cys Ala Ala Ala Cys Thr Gly Cys Thr Gly Ala Ala Thr Gly Ala Ala
85 90 95
Cys Ala Gly Gly Thr Gly Ala Ala Cys Ala Ala Ala Gly Ala Ala Ala
100 105 110
Thr Gly Ala Ala Thr Ala Gly Cys Ala Gly Cys Ala Ala Cys Cys Thr
115 120 125
Gly Thr Ala Thr Ala Thr Gly Ala Gly Cys Ala Thr Gly Ala Gly Cys
130 135 140
Ala Gly Cys Thr Gly Gly Thr Gly Thr Thr Ala Thr Ala Cys Cys Cys
145 150 155 160
Ala Thr Gly Gly Thr Ala Gly Thr Cys Ala Gly Ala Ala Thr Cys Gly
165 170 175
Thr Thr Thr Thr Gly Ala Ala Ala Gly Cys Ala Gly Thr Gly Ala Ala
180 185 190
Gly Ala Gly Gly Gly Thr Ala Gly Cys Ala Gly Cys Cys Thr Gly Gly
195 200 205
Ala Thr Gly Gly Thr Gly Cys Ala Gly Gly Thr Cys Thr Gly Thr Thr
210 215 220
Thr Cys Thr Gly Thr Thr Thr Gly Ala Thr Cys Ala Thr Gly Cys Ala
225 230 235 240
Gly Cys Cys Gly Ala Ala Gly Ala Ala Thr Ala Thr Gly Ala Gly Cys
245 250 255
Ala Cys Gly Cys Ala Ala Ala Ala Ala Ala Ala Cys Thr Gly Ala Thr
260 265 270
Cys Ala Thr Cys Thr Thr Cys Cys Thr Gly Ala Ala Thr Gly Ala Ala
275 280 285
Ala Ala Thr Ala Ala Thr Gly Gly Cys Ala Gly Cys Gly Cys Cys Ala
290 295 300
Cys Cys Ala Ala Ala Cys Ala Thr Ala Ala Ala Ala Thr Thr Cys Cys
305 310 315 320
Gly Gly Thr Thr Ala Ala Ala Gly Gly Thr Ala Gly Cys Gly Cys Ala
325 330 335
Cys Cys Gly Cys Thr Gly Ala Cys Cys Ala Ala Ala Cys Cys Gly Cys
340 345 350
Thr Gly Gly Gly Thr Ala Gly Cys Thr Thr Thr Cys Thr Gly Thr Ala
355 360 365
Thr Thr Ala Thr Gly Cys Ala Gly Thr Thr Cys Cys Gly Gly Thr Thr
370 375 380
Cys Ala Gly Thr Thr Ala Gly Gly Thr Ala Gly Cys Gly Gly Cys Ala
385 390 395 400
Cys Cys Ala Ala Thr Thr Thr Thr Gly Thr Thr Thr Ala Thr Gly Gly
405 410 415
Thr Ala Gly Cys Ala Cys Cys Ala Gly Cys Ala Thr Thr Ala Gly Thr
420 425 430
Gly Cys Ala Cys Cys Gly Gly Ala Ala Cys Ala Thr Ala Ala Ala Thr
435 440 445
Thr Thr Gly Ala Ala Gly Gly Thr Cys Thr Gly Ala Cys Cys Cys Ala
450 455 460
Gly Ala Thr Thr Thr Thr Cys Cys Ala Gly Ala Ala Ala Gly Cys Cys
465 470 475 480
Thr Ala Thr Gly Ala Ala Cys Ala Thr Gly Ala Ala Cys Ala Gly Cys
485 490 495
Ala Thr Ala Thr Thr Ala Gly Cys Gly Ala Gly Ala Gly Cys Ala Thr
500 505 510
Cys Ala Ala Cys Ala Ala Cys Ala Thr Thr Gly Thr Gly Gly Ala Thr
515 520 525
Cys Ala Thr Gly Cys Ala Ala Thr Thr Ala Ala Ala Ala Gly Cys Gly
530 535 540
Gly Thr Thr Cys Ala Gly Gly Thr Ala Cys Gly Ala Ala Cys Thr Thr
545 550 555 560
Thr Gly Thr Gly Thr Ala Thr Gly Gly Thr Thr Cys Ala Thr Gly Thr
565 570 575
Thr Thr Thr Gly Gly Thr Cys Gly Thr Ala Ala Ala Ala Thr Gly Gly
580 585 590
Ala Thr Cys Gly Thr Ala Thr Thr Gly Gly Thr Ala Gly Cys Gly Cys
595 600 605
Cys Ala Ala Thr Thr Ala Thr Ala Thr Cys Gly Gly Cys Ala Gly Cys
610 615 620
Gly Ala Thr Ala Ala Ala Thr Ala Thr Gly Gly Cys Ala Gly Cys Ala
625 630 635 640
Ala Ala Gly Ala Thr Cys Ala Thr Gly Cys Cys Ala Cys Cys Thr Thr
645 650 655
Thr Ala Ala Cys Thr Thr Thr Cys Thr Gly Cys Ala Gly Thr Gly Gly
660 665 670
Thr Ala Thr Gly Thr Thr Gly Cys Ala Gly Ala Ala Cys Ala Gly Cys
675 680 685
Ala Thr Gly Ala Ala Gly Ala Ala Gly Ala Ala Gly Thr Gly Cys Thr
690 695 700
Gly Thr Thr Thr Ala Ala Ala Gly Ala Cys Ala Thr Cys Cys Thr Gly
705 710 715 720
Gly Ala Thr Ala Ala Ala Ala Thr Thr Gly Ala Ala Cys Thr Gly Ala
725 730 735
Thr Cys Gly Gly Cys Ala Ala Thr Gly Ala Ala Ala Ala Cys Cys Ala
740 745 750
Thr Gly Gly Thr Cys Thr Gly Thr Ala Thr Cys Thr Gly Gly Cys Ala
755 760 765
Gly Ala Thr Cys Ala Gly Thr Ala Thr Gly Thr Gly Ala Ala Ala Gly
770 775 780
Gly Thr Ala Thr Thr Gly Cys Cys Ala Ala Ala Ala Gly Cys Cys Gly
785 790 795 800
Cys Ala Ala Ala Ala Gly Cys Thr Ala Ala Cys Thr Cys Gly Ala Gly
805 810 815
<210> 12
<211> 171
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 12
Xaa Xaa Xaa Xaa Met Leu Ser Lys Asp Ile Ile Lys Leu Leu Asn Glu
1 5 10 15
Gln Val Asn Lys Glu Met Asn Ser Ser Asn Leu Tyr Met Ser Met Ser
20 25 30
Ser Trp Cys Tyr Thr His Ser Leu Asp Gly Ala Gly Leu Phe Leu Phe
35 40 45
Asp His Ala Ala Glu Glu Tyr Glu His Ala Lys Lys Leu Ile Ile Phe
50 55 60
Leu Asn Glu Asn Asn Val Pro Val Gln Leu Thr Ser Ile Ser Ala Pro
65 70 75 80
Glu His Lys Phe Glu Gly Leu Thr Gln Ile Phe Gln Lys Ala Tyr Glu
85 90 95
His Glu Gln His Ile Ser Glu Ser Ile Asn Asn Ile Val Asp His Ala
100 105 110
Ile Lys Ser Lys Asp His Ala Thr Phe Asn Phe Leu Gln Trp Tyr Val
115 120 125
Ala Glu Gln His Glu Glu Glu Val Leu Phe Lys Asp Ile Leu Asp Lys
130 135 140
Ile Glu Leu Ile Gly Asn Glu Asn His Gly Leu Tyr Leu Ala Asp Gln
145 150 155 160
Tyr Val Lys Gly Ile Ala Lys Ser Arg Lys Ser
165 170
<210> 13
<211> 116
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 13
Ala Pro Phe Arg Ser Ala Leu Glu Ser Ser Pro Ala Asp Pro Ala Thr
1 5 10 15
Leu Ser Glu Asp Glu Ala Arg Leu Leu Leu Ala Ala Leu Val Gln Asp
20 25 30
Tyr Val Gln Met Lys Ala Ser Glu Leu Glu Gln Glu Gln Glu Arg Glu
35 40 45
Gly Ser Ser Leu Asp Ser Pro Arg Ser Lys Arg Cys Gly Asn Leu Ser
50 55 60
Thr Cys Met Leu Gly Thr Tyr Thr Gln Asp Phe Asn Lys Phe His Thr
65 70 75 80
Phe Pro Gln Thr Ala Ile Gly Val Gly Ala Pro Gly Lys Lys Arg Asp
85 90 95
Met Ser Ser Asp Leu Glu Arg Asp His Arg Pro His Val Ser Met Pro
100 105 110
Gln Asn Ala Asn
115
Claims (2)
1.一种用于临床诊断的复合质控品，其特征在于，包括铁蛋白和与铁蛋白通过连接酯链接的若干个诊断标志物特异性表位多肽分子；
所述诊断标志物特异性表位多肽分子为以下任意一种或多种：
PCT的诊断标志物特异性表位多肽MKASEL、
IL6的诊断标志物特异性表位多肽QNRFESSEEQ、
Myo的诊断标志物特异性表位多肽ATKHKIPVK、
CRP的诊断标志物特异性表位多肽APLTKPL、
PGI的诊断标志物特异性表位多肽GSFLYYA、
PGII的诊断标志物特异性表位多肽GTNFVY、
BNP的诊断标志物特异性表位多肽CFGRKMDRI、
或SAA的诊断标志物特异性表位多肽为ANYIGSDKY；
所述的连接酯为谷氨酸-谷氨酸-谷氨酸-丝氨酸。
2.权利要求1所述的用于临床诊断的复合质控品的制备方法，其特征在于，包括以下步骤：
S1、将诊断标志物蛋白的优势表位定位出来；
S2、将S1中获取的诊断标志物蛋白的优势表位通过连接酯链接到铁蛋白分子上，形成复合物序列；
S3、将该复合物序列合成出来并在体外进行原核大肠杆菌重组表达，即得含有多个诊断标志物优势表位多肽分子的复合抗原。
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114350708A (zh) * | 2022-01-17 | 2022-04-15 | 巴迪泰（广西）生物科技有限公司 | 融合表达蛋白及其制备方法和用途 |
| CN117106102A (zh) * | 2023-10-23 | 2023-11-24 | 中国医学科学院医学生物学研究所 | 肠道病毒多抗原表位融合蛋白、基因、疫苗及其制备方法 |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014143977A2 (en) * | 2013-03-15 | 2014-09-18 | Sera Prognostics, Inc. | Biomarkers and methods for predicting preeclampsia |
| KR20150085700A (ko) * | 2014-01-16 | 2015-07-24 | 한국과학기술연구원 | 표적-특이적 프로브와 표지물질-항체 복합체를 포함하는 바이오마커 탐지용 조성물 및 이의 이용방법 |
| CN105801706A (zh) * | 2014-12-31 | 2016-07-27 | 深圳先进技术研究院 | 蝎氯毒素多肽-铁蛋白重链融合蛋白、自组装蛋白质纳米笼及其制备方法和应用 |
| WO2019118770A1 (en) * | 2017-12-14 | 2019-06-20 | Salk Institute For Biological Studies | Genetically encoded fluorescent-iron ferritin nanoparticle probes for detecting an intracellular target by fluorescent and electron microscopy |
| CN110272500A (zh) * | 2019-07-09 | 2019-09-24 | 中国科学院地质与地球物理研究所 | 一种展示抗体的铁蛋白纳米材料及其制备方法和应用 |
| WO2019195316A1 (en) * | 2018-04-03 | 2019-10-10 | Sanofi | Ferritin proteins |
| WO2020180144A1 (ko) * | 2019-03-07 | 2020-09-10 | 경북대학교 산학협력단 | 트레일 트라이머와 암표적 펩타이드를 멀티디스플레이하는 페리틴 나노케이지 및 이의 항암제로서의 용도 |
-
2020
- 2020-11-23 CN CN202011316538.5A patent/CN112094355B/zh active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014143977A2 (en) * | 2013-03-15 | 2014-09-18 | Sera Prognostics, Inc. | Biomarkers and methods for predicting preeclampsia |
| KR20150085700A (ko) * | 2014-01-16 | 2015-07-24 | 한국과학기술연구원 | 표적-특이적 프로브와 표지물질-항체 복합체를 포함하는 바이오마커 탐지용 조성물 및 이의 이용방법 |
| CN105801706A (zh) * | 2014-12-31 | 2016-07-27 | 深圳先进技术研究院 | 蝎氯毒素多肽-铁蛋白重链融合蛋白、自组装蛋白质纳米笼及其制备方法和应用 |
| WO2019118770A1 (en) * | 2017-12-14 | 2019-06-20 | Salk Institute For Biological Studies | Genetically encoded fluorescent-iron ferritin nanoparticle probes for detecting an intracellular target by fluorescent and electron microscopy |
| WO2019195316A1 (en) * | 2018-04-03 | 2019-10-10 | Sanofi | Ferritin proteins |
| WO2020180144A1 (ko) * | 2019-03-07 | 2020-09-10 | 경북대학교 산학협력단 | 트레일 트라이머와 암표적 펩타이드를 멀티디스플레이하는 페리틴 나노케이지 및 이의 항암제로서의 용도 |
| CN110272500A (zh) * | 2019-07-09 | 2019-09-24 | 中国科学院地质与地球物理研究所 | 一种展示抗体的铁蛋白纳米材料及其制备方法和应用 |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114350708A (zh) * | 2022-01-17 | 2022-04-15 | 巴迪泰（广西）生物科技有限公司 | 融合表达蛋白及其制备方法和用途 |
| CN114350708B (zh) * | 2022-01-17 | 2024-02-13 | 巴迪泰（广西）生物科技有限公司 | 融合表达蛋白及其制备方法和用途 |
| CN117106102A (zh) * | 2023-10-23 | 2023-11-24 | 中国医学科学院医学生物学研究所 | 肠道病毒多抗原表位融合蛋白、基因、疫苗及其制备方法 |
| CN117106102B (zh) * | 2023-10-23 | 2024-02-06 | 中国医学科学院医学生物学研究所 | 肠道病毒多抗原表位融合蛋白、基因、疫苗及其制备方法 |
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