CN112088994A - Ampelopsis grossedentata leaf anti-fatigue beverage and preparation method thereof - Google Patents
Ampelopsis grossedentata leaf anti-fatigue beverage and preparation method thereof Download PDFInfo
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- CN112088994A CN112088994A CN202010844771.4A CN202010844771A CN112088994A CN 112088994 A CN112088994 A CN 112088994A CN 202010844771 A CN202010844771 A CN 202010844771A CN 112088994 A CN112088994 A CN 112088994A
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- ampelopsis grossedentata
- grossedentata leaf
- fatigue
- water
- fatigue beverage
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- 241001018563 Nekemias grossedentata Species 0.000 title claims abstract description 82
- 235000013361 beverage Nutrition 0.000 title claims abstract description 45
- 230000002929 anti-fatigue Effects 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 claims abstract description 58
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 34
- 239000000284 extract Substances 0.000 claims abstract description 33
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 claims abstract description 28
- 229960003692 gamma aminobutyric acid Drugs 0.000 claims abstract description 28
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims abstract description 23
- 239000001768 carboxy methyl cellulose Substances 0.000 claims abstract description 23
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims abstract description 23
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims abstract description 23
- 239000000230 xanthan gum Substances 0.000 claims abstract description 23
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- 229940082509 xanthan gum Drugs 0.000 claims abstract description 23
- 235000010493 xanthan gum Nutrition 0.000 claims abstract description 23
- 239000003765 sweetening agent Substances 0.000 claims abstract description 18
- 239000001509 sodium citrate Substances 0.000 claims abstract description 16
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 16
- 235000003599 food sweetener Nutrition 0.000 claims abstract description 13
- 239000004386 Erythritol Substances 0.000 claims description 9
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 claims description 9
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 claims description 9
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- 229940009714 erythritol Drugs 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 7
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- 235000019202 steviosides Nutrition 0.000 claims description 6
- 229930189775 mogroside Natural products 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 4
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- 238000001816 cooling Methods 0.000 claims description 3
- 238000007872 degassing Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000011049 filling Methods 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 claims description 2
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 239000000811 xylitol Substances 0.000 claims description 2
- 235000010447 xylitol Nutrition 0.000 claims description 2
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims description 2
- 229960002675 xylitol Drugs 0.000 claims description 2
- 239000004383 Steviol glycoside Substances 0.000 claims 1
- 229930182488 steviol glycoside Natural products 0.000 claims 1
- 235000019411 steviol glycoside Nutrition 0.000 claims 1
- 150000008144 steviol glycosides Chemical class 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 9
- 230000002708 enhancing effect Effects 0.000 abstract description 4
- 230000036039 immunity Effects 0.000 abstract description 4
- 230000003647 oxidation Effects 0.000 abstract description 4
- 238000007254 oxidation reaction Methods 0.000 abstract description 4
- 235000013305 food Nutrition 0.000 abstract description 3
- 239000008213 purified water Substances 0.000 description 11
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- 239000000047 product Substances 0.000 description 5
- 229940013618 stevioside Drugs 0.000 description 5
- OHHNJQXIOPOJSC-UHFFFAOYSA-N stevioside Natural products CC1(CCCC2(C)C3(C)CCC4(CC3(CCC12C)CC4=C)OC5OC(CO)C(O)C(O)C5OC6OC(CO)C(O)C(O)C6O)C(=O)OC7OC(CO)C(O)C(O)C7O OHHNJQXIOPOJSC-UHFFFAOYSA-N 0.000 description 5
- 235000021092 sugar substitutes Nutrition 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
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- 206010061218 Inflammation Diseases 0.000 description 2
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 206010068319 Oropharyngeal pain Diseases 0.000 description 1
- 241000219099 Parthenocissus quinquefolia Species 0.000 description 1
- 201000007100 Pharyngitis Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
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- 206010012601 diabetes mellitus Diseases 0.000 description 1
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- 230000003203 everyday effect Effects 0.000 description 1
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- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- -1 found in vertebrates Natural products 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
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- 229920001282 polysaccharide Polymers 0.000 description 1
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- 210000002966 serum Anatomy 0.000 description 1
- 210000002027 skeletal muscle Anatomy 0.000 description 1
- 229960001790 sodium citrate Drugs 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
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- 238000012360 testing method Methods 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/60—Sweeteners
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/62—Clouding agents; Agents to improve the cloud-stability
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Botany (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses an anti-fatigue beverage prepared from Ampelopsis grossedentata leaves and a preparation method thereof. An anti-fatigue beverage containing Ampelopsis grossedentata leaves comprises the following components: the components of the gum comprise Ampelopsis grossedentata leaf extract, sodium citrate, water, sodium carboxymethylcellulose, xanthan gum, gamma-aminobutyric acid and a sweetening agent. The ampelopsis grossedentata leaf anti-fatigue beverage provided by the invention is mainly prepared from new resource food, is matched with components such as gamma-aminobutyric acid (GABA) and the like, and has the effects of eliminating fatigue, relieving pressure, enhancing immunity, resisting oxidation and the like.
Description
The technical field is as follows:
the invention relates to the technical field of plant beverages, and particularly relates to an anti-fatigue beverage prepared from Ampelopsis grossedentata leaves and a preparation method thereof.
Background art:
ampelopsis grossedentata is a Vitaceae ampelopsis plant, and the leaves of Ampelopsis grossedentata are approved by the former national health committee of 12 months and 24 days in 2013 to be a new resource food. The Ampelopsis grossedentata leaves and the extract thereof are rich in flavone, polysaccharide, various trace elements and amino acid, and have the effects of clearing away heat and toxic materials, enhancing resistance, resisting inflammation, inhibiting bacteria, resisting oxidation, enhancing immunity and the like. GABA, globally known as gamma-aminobutyric acid, is a non-proteinogenic amino acid, mainly found in vertebrates, plants and microorganisms. GABA is used as an inhibitory neurotransmitter in human body and has various effects of improving sleep, promoting relaxation, lowering blood pressure, relieving fatigue, improving emotion and the like. Sugar substitutes have a relatively high sweetness and low self-caloric content and can be used in products to reduce caloric intake. In addition, part of sugar substitutes have little influence on blood sugar and are very friendly to diabetes patients.
Although the beverage in the market is various, the demand of consumers for the anti-fatigue type beverage is high. The natural and healthy components are selected to create the product with the functions of eliminating fatigue, relieving pressure, resisting oxidation, improving immunity and reducing blood pressure for consumers, and the product has great significance.
The invention content is as follows:
the invention aims to overcome the defects of the prior art and provide the ampelopsis grossedentata leaf anti-fatigue beverage and the preparation method thereof.
The invention aims to provide an anti-fatigue beverage containing Ampelopsis grossedentata leaves, which comprises the following components: ampelopsis grossedentata leaf extract, sodium citrate, water, sodium carboxymethylcellulose, xanthan gum, gamma-aminobutyric acid (GABA) and a sweetener.
The invention provides an anti-fatigue beverage of Ampelopsis grossedentata leaves, which takes Ampelopsis grossedentata leaves and GABA as main functional raw materials. The Ampelopsis grossedentata leaf extract has the effects of enhancing immunity, resisting oxidation, clearing away heat and toxic materials, relieving sore throat, resisting inflammation and the like. GABA has effects of improving sleep, promoting relaxation, lowering blood pressure, relieving fatigue, improving emotion, etc., and has synergistic effect of Ampelopsis grossedentata leaf extract and GABA to greatly improve antifatigue effect. In addition, the product selects sugar substitute as sweetener, which can reduce calorie intake and control blood sugar level well.
Preferably, the ampelopsis grossedentata leaf anti-fatigue beverage comprises the following components in parts by mass: 0.1-1 part of ampelopsis grossedentata leaf extract, 0.05-0.3 part of sodium citrate, 400-700 parts of water, 0.01-0.5 part of sodium carboxymethylcellulose, 0.01-0.5 part of xanthan gum, 0.01-0.1 part of gamma-aminobutyric acid and 1-4 parts of sweetener. After the sodium carboxymethylcellulose and the xanthan gum are compounded and added into the anti-fatigue beverage of the Ampelopsis grossedentata leaves, the stability of a solution system is improved, so that the anti-fatigue beverage of the Ampelopsis grossedentata leaves is clearer and more transparent. The anti-fatigue beverage of ampelopsis grossedentata leaves is prepared by selecting pure water as the component.
Further preferably, the coating comprises the following components in parts by mass: 0.15-0.8 part of ampelopsis grossedentata leaf extract, 0.05-0.3 part of sodium citrate, 500-600 parts of water, 0.01-0.2 part of sodium carboxymethylcellulose, 0.01-0.1 part of xanthan gum, 0.02-0.07 part of gamma-aminobutyric acid and 1.7-2.6 parts of sweetener.
Preferably, the sweetener is selected from more than one of mogroside, stevioside, xylitol and erythritol.
The invention also protects a preparation method of the ampelopsis grossedentata leaf anti-fatigue beverage, which comprises the following steps:
(1) dissolving Ampelopsis grossedentata leaf extract in a part of water, and collecting supernatant to obtain Ampelopsis grossedentata leaf extract solution;
(2) adding the sodium carboxymethylcellulose and the xanthan gum into the rest water to be completely dissolved to obtain a solution of the sodium carboxymethylcellulose and the xanthan gum;
(3) and (3) uniformly mixing and dissolving the ampelopsis grossedentata leaf extract solution obtained in the step (1) and the solution of sodium carboxymethylcellulose and xanthan gum obtained in the step (2) with sodium citrate, gamma-aminobutyric acid and a sweetening agent, homogenizing, degassing, carrying out instantaneous sterilization at an ultra-high temperature (the sterilization temperature is 135 ℃ and the sterilization time is 10s), cooling and filling to obtain the ampelopsis grossedentata leaf anti-fatigue beverage.
Preferably, the temperature of the water in the step (1) is 80-100 ℃.
Preferably, the ampelopsis grossedentata leaf extract in the step (1) is prepared by the following steps: cleaning Ampelopsis grossedentata leaves, removing impurities, extracting with a solvent, filtering, concentrating, drying, and pulverizing or granulating to obtain Ampelopsis grossedentata leaf extract.
The ampelopsis grossedentata leaf extract is prepared by the following steps:
1. selecting materials: selecting qualified and high-quality dried ampelopsis grossedentata leaves, and preventing mildew, worm damage and the like;
2. extraction: washing Ampelopsis grossedentata leaves with running water, removing surface soil impurities and the like, putting the washed medicinal materials into an extraction container, and adding water, wherein the liquid-material ratio is 1: 10-1: 15, placing the mixture in a constant-temperature water bath kettle for leaching at the extraction temperature of 90-100 ℃, filtering the mixture by using multilayer gauze after extracting for 2-3h, adding 8-12 times of water into filter residue, extracting for 2-3h again at the extraction temperature of 90-100 ℃, filtering and combining extracting solutions, and concentrating the extracting solutions under reduced pressure to obtain a certain amount of concentrated solution.
3. And (3) drying: the concentrated solution is dried by adopting a spray drying mode, the air inlet temperature is 150-170 ℃, and the air outlet temperature is 90-95 ℃.
The invention has the beneficial effects that:
(1) the invention utilizes the characteristics and advantages of a new resource food, namely the Ampelopsis grossedentata leaves, simultaneously solves the problem of active ingredient precipitation caused by long-time storage, and is a plant beverage with multiple effects of relieving fatigue, reducing blood pressure, improving emotion, promoting relaxation and the like;
(2) the sugar substitute is selected as a sweetening agent, so that the sugar substitute is beneficial to controlling the rise of blood sugar and can reduce the intake of calories.
The specific implementation mode is as follows:
the following examples are further illustrative of the present invention and are not intended to be limiting thereof.
Unless otherwise specified, the devices and materials mentioned in the present invention are commercially available.
Example 1
An anti-fatigue beverage containing Ampelopsis grossedentata leaves comprises the following components: 0.5g of ampelopsis grossedentata leaf extract, 0.2g of sodium citrate, 600g of purified water, 0.1g of sodium carboxymethylcellulose, 0.05g of xanthan gum, 0.03g of GABA, 2g of erythritol and 0.15g of stevioside. The preparation method of the ampelopsis grossedentata leaf anti-fatigue beverage comprises the following steps:
(1) adding 0.5g of Ampelopsis grossedentata leaf extract into 550g of 90 ℃ purified water to completely dissolve the Ampelopsis grossedentata leaf extract, centrifuging and taking supernatant to obtain Ampelopsis grossedentata leaf extract solution;
(2) adding 0.1g of sodium carboxymethylcellulose and 0.05g of xanthan gum into 50g of purified water for complete dissolution to obtain a solution of the sodium carboxymethylcellulose and the xanthan gum;
(3) and (3) uniformly mixing and dissolving the ampelopsis grossedentata leaf extract solution obtained in the step (1), the sodium carboxymethylcellulose and xanthan gum solution obtained in the step (2), sodium citrate, GABA, erythritol and stevioside, homogenizing, degassing, carrying out ultrahigh temperature instantaneous sterilization (the sterilization temperature is 135 ℃, the sterilization time is 10s), cooling and filling to obtain the ampelopsis grossedentata leaf anti-fatigue beverage finished product.
Example 2
An anti-fatigue beverage containing Ampelopsis grossedentata leaves comprises the following components: 0.8g of ampelopsis grossedentata leaf extract, 0.3g of sodium citrate, 600g of purified water, 0.1g of sodium carboxymethylcellulose, 0.05g of xanthan gum, 0.02g of GABA, 1.5g of erythritol and 0.2g of stevioside.
The preparation method of the ampelopsis grossedentata leaf anti-fatigue beverage is the same as the preparation method of the example 1.
Example 3
An anti-fatigue beverage containing Ampelopsis grossedentata leaves comprises the following components: 0.4g of ampelopsis grossedentata leaf extract, 0.2g of sodium citrate, 500g of purified water, 0.2g of sodium carboxymethylcellulose, 0.1g of xanthan gum, 0.05g of GABA, 0.1g of mogroside and 2.5g of erythritol.
The preparation method of the ampelopsis grossedentata leaf anti-fatigue beverage is the same as the preparation steps of the example 1, except that 450g of purified water is added in the step (1).
Example 4
An anti-fatigue beverage containing Ampelopsis grossedentata leaves comprises the following components: 0.15g of ampelopsis grossedentata leaf extract, 0.05g of sodium citrate, 600g of purified water, 0.01g of sodium carboxymethylcellulose, 0.01g of xanthan gum, 0.07g of GABA, 0.15g of mogroside and 2g of erythritol.
The preparation method of the ampelopsis grossedentata leaf anti-fatigue beverage is the same as the preparation method of the example 1.
Example 5
An anti-fatigue beverage containing Ampelopsis grossedentata leaves comprises the following components: 0.1g of ampelopsis grossedentata leaf extract, 0.05g of sodium citrate, 400g of purified water, 0.01g of sodium carboxymethylcellulose, 0.01g of xanthan gum, 0.01g of GABA, 0.15g of mogroside and 0.85g of erythritol.
The preparation method of the ampelopsis grossedentata leaf anti-fatigue beverage is the same as the preparation steps of the example 1, except that 350g of purified water is added in the step (1).
Example 6
An anti-fatigue beverage containing Ampelopsis grossedentata leaves comprises the following components: 1g of ampelopsis grossedentata leaf extract, 0.3g of sodium citrate, 700g of purified water, 0.5g of sodium carboxymethylcellulose, 0.5g of xanthan gum, 0.1g of GABA, 3.8g of erythritol and 0.2g of stevioside.
The preparation method of the ampelopsis grossedentata leaf anti-fatigue beverage is the same as the preparation steps of the example 1, except that 650g of purified water is added in the step (1).
Experimental example 1: animal fatigue test of fatigue-resistant Ampelopsis grossedentata leaf beverage obtained in example 4
1. Laboratory animal
40 healthy male KM mice, weighing 18-22g, were bred adaptively for 5 days at room temperature of 23-25 ℃ and relative humidity of 55-60% before the experiment. Alternate light and shade for 12h, freely drink water, freely eat standard feed, and the treatment of the animal in the experimental process accords with the animal ethical standard.
2. Experimental groups and methods of administration
40 mice were randomly divided into 4 groups, which were a normal group, a GABA group, a Ampelopsis grossedentata aqueous extract group, and an anti-fatigue drink group, 10 mice per group. The normal group does not do anything. The administration dose of the mice was obtained by converting the equivalent dose according to the body surface area, and the administration was carried out in a 10-fold manner as compared with the equivalent dose. The GABA group and the Ampelopsis grossedentata water extract group are respectively and independently administered to mice, and the independent administration amount is the same as the GABA or the extract in the anti-fatigue beverage. The administration method comprises the following steps: the administration is carried out by gavage for 1 time at 9 am every day for 30 days, and normal group is gavage with normal saline with corresponding volume.
3. Observation indexes are as follows:
3.1 judge the fatigue degree of the mice by the time of exhaustion of swimming. The mice were given a load of 6% and were forced to swim to exhaustion, and were judged to be exhausted when they were submerged in water for 10 seconds and could not emerge from the water surface, and the exhaustion swimming time was recorded.
3.2 serum urea, lactate, myoglycogen assay: collecting blood by an eyeball-picking method 1h after the mouse exhaustive swimming is finished, collecting the blood into a centrifuge tube, standing for 1h, centrifuging for 10min at 4 ℃ 4000r/min, taking supernatant, and storing in a refrigerator at-80 ℃ for later use. The left hind leg skeletal muscle was collected from dissected mice and stored in a freezer at-80 ℃ for future use. Detection was performed according to the kit instructions.
Data were processed using software GaphPad Prism 8.0.2, Kolmogorov-Smirnov test for normal test, and Brown-Forsythe test for homogeneity of variance. One-way ANOVA is adopted for data which are in accordance with normal distribution and uniform in variance, and Tukey is adopted for pairwise comparison; normal distribution but variable data were obtained using Brown-Forythe and Welch ANOVA, and Dunnett's T3 for pairwise comparisons. Data are expressed as mean ± standard deviation.
The experimental result shows that the beverage of the example 4 has obvious anti-fatigue effect, the anti-fatigue effect is stronger than that of singly taking the ampelopsis grossedentata aqueous extract or GABA, and the ampelopsis grossedentata aqueous extract and GABA are compounded to show a certain synergistic effect.
Table 1: experiment of exhaustive swimming time and Biochemical index level of mice in each group
Note: p < 0.05, P < 0.01, compared to normal group.
The present invention provides a fatigue-resistant beverage prepared from Ampelopsis grossedentata leaves and a method for preparing the same, and the above description is only for the purpose of helping understanding the technical solution and the core idea of the present invention, it should be noted that, for those skilled in the art, it is possible to make various modifications and alterations of the present invention without departing from the principle of the present invention, and the modifications and alterations are also within the protection scope of the present invention.
Claims (8)
1. The ampelopsis grossedentata leaf anti-fatigue beverage is characterized by comprising the following components: the components of the gum comprise Ampelopsis grossedentata leaf extract, sodium citrate, water, sodium carboxymethylcellulose, xanthan gum, gamma-aminobutyric acid and a sweetening agent.
2. The ampelopsis grossedentata leaf anti-fatigue beverage according to claim 1, which comprises the following components in parts by mass: 0.1-1 part of ampelopsis grossedentata leaf extract, 0.05-0.3 part of sodium citrate, 400-700 parts of water, 0.01-0.5 part of sodium carboxymethylcellulose, 0.01-0.5 part of xanthan gum, 0.01-0.1 part of gamma-aminobutyric acid and 1-4 parts of sweetener.
3. The ampelopsis grossedentata leaf anti-fatigue beverage according to claim 2, which comprises the following components in parts by mass: 0.15-0.8 part of ampelopsis grossedentata leaf extract, 0.05-0.3 part of sodium citrate, 500-600 parts of water, 0.01-0.2 part of sodium carboxymethylcellulose, 0.01-0.1 part of xanthan gum, 0.02-0.07 part of gamma-aminobutyric acid and 1.7-2.6 parts of sweetener.
4. The ampelopsis grossedentata leaf anti-fatigue beverage according to claim 1 or 2, wherein the sweetener is selected from one or more of mogrosides, steviol glycosides, xylitol, and erythritol.
5. The preparation method of the ampelopsis grossedentata leaf anti-fatigue beverage as claimed in claim 1, which is characterized by comprising the following steps:
(1) dissolving Ampelopsis grossedentata leaf extract in a part of water, and collecting supernatant to obtain Ampelopsis grossedentata leaf extract solution;
(2) adding the sodium carboxymethylcellulose and the xanthan gum into the rest water to be completely dissolved to obtain a solution of the sodium carboxymethylcellulose and the xanthan gum;
(3) and (3) uniformly mixing and dissolving the ampelopsis grossedentata leaf extract solution obtained in the step (1) and the sodium carboxymethylcellulose and xanthan gum dissolved solution obtained in the step (2) with sodium citrate, gamma-aminobutyric acid and a sweetening agent, homogenizing, degassing, sterilizing, cooling and filling to obtain the ampelopsis grossedentata leaf anti-fatigue beverage.
6. The method for preparing the anti-fatigue beverage from the Ampelopsis grossedentata leaves as claimed in claim 5, wherein the temperature of the water in the step (1) is 80-100 ℃.
7. The preparation method of the ampelopsis grossedentata leaf anti-fatigue beverage according to claim 5, wherein the ampelopsis grossedentata leaf extract in the step (1) is prepared by the following steps: cleaning Ampelopsis grossedentata leaves, removing impurities, extracting with a solvent, filtering, concentrating, drying, and pulverizing or granulating to obtain Ampelopsis grossedentata leaf extract.
8. The method for preparing the anti-fatigue beverage from the ampelopsis grossedentata leaves as claimed in claim 7, wherein the solvent is water.
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