CN112041333A - 新型融合蛋白和用于预防或治疗癌症的包含该融合蛋白的药物组合物 - Google Patents

新型融合蛋白和用于预防或治疗癌症的包含该融合蛋白的药物组合物 Download PDF

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CN112041333A
CN112041333A CN201980028463.XA CN201980028463A CN112041333A CN 112041333 A CN112041333 A CN 112041333A CN 201980028463 A CN201980028463 A CN 201980028463A CN 112041333 A CN112041333 A CN 112041333A
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金廷镐
金范锡
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Good T Cells Inc
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Abstract

本发明涉及融合蛋白，其包含富含亮氨酸和免疫球蛋白样结构域‑1(Lrig‑1)蛋白的细胞外结构域和免疫球蛋白Fc区。本发明提供的融合蛋白能与效应T细胞中存在的Lrig‑1蛋白的配体相互作用，以致基于抑制在其表面上包含Lrig‑1蛋白的调节性T细胞与效应T细胞之间的相互作用，从而抑制调节性T细胞(Treg细胞)的活性并维持或促进效应T细胞的活性，因此有效地抑制癌细胞，特别是实体癌细胞的生长。

Description

新型融合蛋白和用于预防或治疗癌症的包含该融合蛋白的药 物组合物
技术领域
本发明涉及一种新型融合蛋白及其用途，所述融合蛋白包含富含亮氨酸和免疫球蛋白样结构域1(Lrig-1)蛋白的胞外结构域和免疫球蛋白Fc区。
背景技术
免疫球蛋白包含四个多肽链，即两个重链和两个轻链，它们通过链间二硫键相互结合。每条轻链具有两个结构域，即可变轻链结构域(VL)和恒定轻链结构域(CL)。每个重链具有两个区域，即可变重链区(VH)和恒定重链区(CH)。恒定重链区(CH)由以数字指定的恒定重区域(例如，CH1，CH2，CH3等)组成(例如，参见US 6,086,875(Blumberg R.S.等人)、US5,624,821(Winter G.P.等人)和US 5,116,964(Capon D.J.和Lasky L.A.))。根据免疫球蛋白的生物学特性，在生物体内的位置以及加工不同抗原的能力，它们可以分为不同的同种型(即IgG、IgM、IgA、IgD和IgE)。取决于免疫球蛋白同种型，恒定重链区(CH)可以具有3个或4个CH结构域。另外，在某些同种型(IgA、IgD和IgG)中，重链包含铰链区，该铰链区增加了分子的柔韧性(Janeway等人，2001，Immunobiology，加兰出版社，纽约，纽约州)。
在人类中，有四个IgG亚类(IgG1、2、3和4)，它们以其在血清中的丰度顺序命名(IgG1最丰富)。IgG同种型由两条轻链和两条重链组成，其中每条重链均包含三个恒定重链结构域(CH1、CH2和CH3)。IgG的两条重链通过二硫键(-S-S-)相互连接，每条重链均通过二硫键与轻链相连。IgG的抗原结合位点位于片段抗原结合区(Fab区)中，该片段包括轻链(VL)和重链(VH)的可变结构域以及轻链(CL)和重链(CH1)的恒定结构域域。IgG的片段可结晶区(Fc区)是包含CH2和CH3结构域的重链的部分，其与某些细胞表面上的Fc受体结合，包括新生Fc受体(FcRn)。IgG的重链在CH1和CH2之间还具有一铰链区(铰链)，可将Fab区与Fc区分开，并参与通过二硫键连接两条重链。铰链区的结构有助于四种IgG亚类的每一个的独特生物学特性。
IgG是作为一种单体被分泌的，它的体积很小，因此很容易遍及组织。它是唯一具有受体(新生儿Fc受体(FcRn))的同种型，容易通过人类胎盘，从而为子宫内胎儿提供。通过胎盘吸收的IgG为新生儿在其自身免疫系统发育之前提供了体液免疫。
IgG新生儿Fc受体(FcRn)结合位点位于抗体的Fc区。FcRn通常在人胎盘和上皮细胞中表达，并参与阻止IgG降解的内吞挽救途径。在酸性pH下，IgG与FcRn的高度pH依赖结合亲和力介导了这种挽救途径。在酸性pH下，IgG对FcRn的高亲和力被认为导致内在化的IgG与FcRn结合，随后被吸收到酸性核内体中(Goebl N A等人，2008；Junghans R P等人，1996)。内在化后，大多数可溶性蛋白直接针对溶酶体。但是，内在化的FcRn结合IgG返回质膜，并有效地从潜在的降解途径中拯救出来。暴露于细胞外空间的中性pH后，IgG可从FcRn解离并返回循环。因此，抗体的延长的血清半衰期特性保留在Fc片段中。
与未修饰的蛋白质药物相比，挽救途径为开发血液半衰期延长的下一代蛋白质药物提供了一种机制。特别地，未修饰的蛋白质药物的循环半衰期短，因此需要在所需的长期治疗期间内频繁施用。已经付出大量的努力使用包括聚乙二醇化和融合蛋白技术在内的许多方法来延长蛋白药物的半衰期(美国食品和药物管理局；Osborn BL等人，2002)。但是，这些努力并未取得理想的结果。
发明内容
技术问题
本发明的目的是提供一种新型的融合蛋白，包括富含亮氨酸和免疫球蛋白样结构域1(Lrig-1)蛋白的细胞外结构域和免疫球蛋白Fc区。
本发明的另一目的是提供一种核酸分子，其编码根据本发明的融合蛋白。
本发明的又一个目的是提供一种表达载体，其中插入了根据本发明的核酸分子。
本发明的又一个目的是提供用根据本发明的表达载体转染的宿主细胞系。
本发明的又一个目的是提供用于预防或治疗癌症的药物组合物，其包含根据本发明的融合蛋白。
然而，本发明要实现的技术问题不限于上述问题，并且本领域普通技术人员将从以下描述中清楚地理解未提及的其他问题。
问题的解决方法
根据本发明的一实施方式，提供了一种融合蛋白，其包含富含亮氨酸和免疫球蛋白样结构域1(Lrig-1)蛋白的细胞外结构域和免疫球蛋白Fc区。
在本发明中，“Lrig-1蛋白”是由存在于调节性T细胞表面的1091个氨基酸组成的跨膜蛋白，其由富含亮氨酸重复序列(LRR)和细胞外或管腔侧的三个免疫球蛋白样结构域、细胞跨膜序列和细胞质尾部组成。LRIG基因家族包括LRIG1、LRIG2和LRIG3，并且它们之间的氨基酸是高度保守的。
在本发明的一个实例中，Lrig-1蛋白的细胞外结构域可以是衍生自哺乳动物的Lrig-1蛋白的细胞外结构域，所述哺乳动物包括灵长类动物，例如人和猴，啮齿类动物，例如小鼠和大鼠，等等。
在本发明的一个实例中，Lrig-1蛋白的细胞外结构域可以但不限于由SEQ ID NO：1表示，其对应于人源Lrig-1蛋白的氨基酸35至794的序列，并且可以由SEQ ID NO：2表示的核酸序列编码(参见表1)。
表1
Figure BDA0002742983620000021
Figure BDA0002742983620000031
在本发明的另一个实例中，Lrig-1蛋白的细胞外结构域可以但不限于由SEQ IDNO：3表示，其对应于小鼠源的Lrig-1蛋白的氨基酸35至794的序列，并且可以由SEQ ID NO：4表示的核酸序列编码(参见表2)。
表2
Figure BDA0002742983620000032
Figure BDA0002742983620000041
如本文所用，术语“免疫球蛋白Fc区”是指免疫球蛋白的区域，其包括重链恒定区2(CH2)和/或重链恒定区3(CH3)，但不包括重链和轻链可变区。免疫球蛋白Fc区可以是构成本发明的蛋白质组合中的部分的成分。
免疫球蛋白Fc区可在重链恒定区中包括铰链部分，从而不仅影响最终制备的融合蛋白的结构柔性，而且进一步提高了融合蛋白的生产率和稳定性。然而，本发明不限于此。
另外，本发明的免疫球蛋白Fc区可以是免疫球蛋白的延伸Fc区，其包括一些或全部重链恒定区1(CH1)和/或轻链恒定区1(CL1)，仅排除重链和轻链可变区，只要免疫球蛋白Fc区具有与其原初类型相比基本上相同或改善的效果。另外，免疫球蛋白Fc区可以是通过去除对应于CH2和/或CH3的相当长的部分氨基酸序列而获得的区域。
例如，本发明的免疫球蛋白Fc区可以是1)CH1结构域、CH2结构域、CH3结构域和CH4结构域；2)CH1结构域和CH2结构域；3)CH1结构域和CH3结构域；4)CH2结构域和CH3结构域；5)免疫球蛋白铰链区(或铰链区的一部分)与CH1结构域、CH2结构域、CH3结构域和CH4结构域中的一个或两个或多个的组合；和6)由重链恒定区和轻链恒定区的每个结构域形成的二聚体。然而，本发明不限于此。
另外，本发明的免疫球蛋白Fc区包括其原初氨基酸序列及其序列衍生物。氨基酸序列衍生物是指通过缺失、插入，或非保守或保守取代至少一个氨基酸残基或其组合而不同于其原初氨基酸序列的氨基酸序列。
例如，对于IgG Fc，已知对结合很重要的位置214至238、297至299、318至322或327至331的氨基酸残基可用作修饰的合适位点。
另外，各种类型的衍生物是可能的，包括一种通过缺失能够形成二硫键的位点获得的衍生物，一种通过缺失原初Fc的N末端的一些氨基酸残基获得的衍生物，一种通过在原初Fc的N末端添加甲硫氨酸获得的衍生物等。另外，为了去除其中的效应子功能，可以缺失补体结合位点，例如C1q结合位点，或者可以缺失抗体依赖性细胞介导的细胞毒性(ADCC)位点。国际专利公开号WO 97/34631和WO 96/32478等公开了用于制备免疫球蛋白Fc区的此类序列衍生物的技术。
蛋白质和肽中的氨基酸交换整体上不会改变分子活性，这是本领域已知的(H.Neurath，R.L.Hill，蛋白质，学术出版社，纽约，1979)。最常见的交换是在氨基酸残基Ala/Ser、Val/Ile、Asp/Glu、Thr/Ser、Ala/Gly、Ala/Thr、Ser/Asn、Ala/Val、Ser/Gly、Thy/Phe、Ala/Pro、Lys/Arg、Asp/Asn、Leu/Ile、Leu/Val、Ala/Glu或Asp/Gly之间发生的交换。视情况而定，可以通过磷酸化、硫酸化、丙烯酰化、糖基化、甲基化、法尼基化、乙酰化、酰胺化等来实现修饰。
上述Fc衍生物表现出与本发明的Fc区域相同的生物活性，并且可以是具有提高的对热，pH等的结构稳定性的那些。
另外，这些Fc区可以获自体内分离自动物，例如人、牛、山羊、猪、小鼠、兔、仓鼠、大鼠或豚鼠的原初类型，或者可以是重组体，其获自转化的动物细胞或微生物或其衍生物。在此，从原初型获得Fc区的方法可以是这样的方法，其中从活的人或动物体中分离出完整的免疫球蛋白，然后用蛋白酶处理以获得Fc区。在用木瓜蛋白酶处理的情况下，整个免疫球蛋白被切割成Fab和Fc。在用胃蛋白酶处理的情况下，整个免疫球蛋白被切割成pF'c和F(ab)2。可以使用尺寸排阻色谱法等分离Fc或pF'c。在一个更具体的实施方式中，免疫球蛋白Fc区是使用人或小鼠来源的Fc区从微生物获得的重组免疫球蛋白Fc区。
另外，免疫球蛋白Fc区可以具有原初型聚糖，或者与原初型相比聚糖水平升高或降低，或者可以是去糖基化的形式。可以使用诸如化学方法，酶促方法和使用微生物的基因工程方法之类的常规方法来增加或减少免疫球蛋白Fc中的聚糖或去糖基化。在此，其中已在Fc上去除聚糖的免疫球蛋白Fc区表现出与补体(C1q)的结合能力显著降低，并且抗体依赖性细胞毒性或补体依赖性细胞毒性降低或消除。因此不会在体内引起任何不必要的免疫反应。从这个观点来看，去糖基化或非糖基化的免疫球蛋白Fc区将是更适合作为药物载体的最初目的的形式。
如本文所用，术语“去糖基化”是指从其中酶促除去糖的Fc区，并且术语“非糖基化”是指通过原核生物(在更具体的实施方式中是大肠杆菌)中产生的未被糖基化的Fc区。。
同时，免疫球蛋白Fc区可以源自人或其他动物，例如牛、山羊、猪、小鼠、兔、仓鼠、大鼠和豚鼠。在一更具体的实施方式中，其源自人。
另外，本发明的免疫球蛋白Fc区可以是IgG-、IgA-、IgD-、IgE-或IgM-源的Fc区，重链恒定区2(CH2)、重链恒定区3(CH3)、铰链、其片段或其组合、或包括该组合的杂合Fc。
同时，如本文所用，术语“组合”是指编码相同来源的单链免疫球蛋白Fc区，重链恒定区2(CH2)或重链恒定区3(CH3)的多肽，当它们形成二聚体或多聚体时，与不同来源的单链多肽形成键。也就是说，可以从选自IgG-、IgA-、IgD-、IgE-或IgM-源的Fc区，重链恒定区2(CH2)或重链恒定区3(CH3)的两个或多个片段制备二聚体或多聚体链。
在本发明中，“杂合Fc”可以衍生自人IgG亚类的组合或人IgD和IgG的组合。在一个实施方式中，杂合Fc可包括，例如IgD铰链区和CH2 N末端区+IgG4 CH2和CH3区。例如，可以以相同的方式采用并使用在韩国专利号0897938中公开的杂合Fc形式，其通过引用并入本文。在本发明中，在杂合Fc与生物活性分子，多肽等结合的情况下，它具有增加生物活性分子的血清半衰期的作用，以及当多肽通过编码Fc多肽融合蛋白的核苷酸序列表达时，具有增加肽的表达水平的作用。
作为本发明的一实例，免疫球蛋白Fc区可以是IgG-、IgA-、IgM-、IgD-或IgE-源的Fc区，或可以包括IgG-、IgA-、IgM-、IgD-或IgE-源的重链恒定区2(CH2)和重链恒定区3(CH3)。然而，本发明不限于此。
作为本发明的一实例，免疫球蛋白Fc区可以是在人血中最丰富的IgG-或IgM-源的Fc区，或者可以包括IgG-或IgM-源的重链恒定区2(CH2)和重链链恒定区3(CH3)。作为另一个例子，免疫球蛋白Fc区可以是已知可延长配体结合蛋白的半衰期的IgG-源的Fc区，或者可以包括IgG-源的重链恒定区2(CH2)和重链恒定区3(CH3)。作为又一个实例，免疫球蛋白Fc区可以是IgG1-、IgG2-、IgG3-或IgG4-源的Fc区，或可以包括IgG1-、IgG2-、IgG3-或IgG4-源的重链恒定区2(CH2)和重链恒定区3(CH3)。作为又一个实例，免疫球蛋白Fc区可包括IgG1-或IgG2-源的重链恒定区2(CH2)和重链恒定区3(CH3)。
作为本发明的优选实例，免疫球蛋白Fc区可包括但不限于人IgG1-源的重链恒定区2(CH2)和重链恒定区3(CH3)，其由SEQ ID NO：5表示，或小鼠IgG2-源的重链恒定区2(CH2)和重链恒定区3(CH3)，其由SEQ ID NO：6表示(参见下表3)。
表3
Figure BDA0002742983620000061
Figure BDA0002742983620000071
作为本发明的一个实例，免疫球蛋白Fc区可包括IgG-、IgA-、IgM-、IgD-、IgE-或阿巴西普(Abatacept)-源的铰链区；作为另一个例子，免疫球蛋白Fc区可包括IgG\IgD或阿巴西普-源的铰链区，或可包括IgG1-、IgG2-、IgG3-、IgG4-、IgD-或阿巴西普-源的铰链区。然而，本发明不限于此。
作为本发明的优选实例，免疫球蛋白Fc区可包括选自下组中的一或多个：由SEQID NO：7表示的人IgG1-源的铰链区、由SEQ ID NO：8表示的人IgG2-源的铰链区、由SEQ IDNO：9表示的人IgD-源的铰链区和由SEQ ID NO：10表示的阿巴西普的铰链区(参见下面的表4)，从而提高了最终制备的融合蛋白的结构柔性，并显著提高了融合蛋白的生产率和稳定性。然而，本发明不限于此。
表4
SEQ ID NO 序列信息
SEQ ID NO:7 EPKSSDKTHTSPPCPAPELLGGPSVF
SEQ ID NO:8 EPRGPTIKPCPPCKCPAPNLLGGPSVF
SEQ ID NO:9 RNTGRGGEEKKKEKEKEEQEERETKTPECPSHTQPLGVF
SEQ ID NO:10 EPKSSDKTHTSPPSPAPELLGGSSVF
在本发明中，在Lrig-1蛋白的细胞外结构域和Fc区通过接头连接的情况下，该接头可以连接至Fc片段的N末端、C末端或自由基，并且可以与Lrig-1蛋白的细胞外结构域的N末端、C末端或自由基连接。在接头是肽接头的情况下，可以在任何位点发生连接。例如，接头可以与Lrig-1蛋白的细胞外结构域的C末端和免疫球蛋白Fc区域的N末端连接，或者可以与免疫球蛋白Fc区域的C末端和Lrig-1蛋白的细胞外结构域的的N末端连接。
在本发明中，“接头”可以减少融合蛋白中Lrig-1蛋白的细胞外结构域与免疫球蛋白Fc区之间的干扰，从而增加靶细胞中Lrig-1蛋白的细胞外结构域的所需活性。另外，在本发明中，接头可以包括可被在具有靶疾病的组织或细胞中过表达的酶切割的序列。在接头可以被如上所述的过表达的酶切割的情况下，可能有效防止多肽的活性由于Fc部分而降低。
作为本发明的一个例子，该接头优选具有1至100个氨基酸；然而，本发明不限于此。可以使用能够将Lrig-1蛋白的细胞外结构域与免疫球蛋白Fc隔开的任何肽。尽管对构成接头的氨基酸序列没有特别限制，但是优选包括甘氨酸(G)和丝氨酸(S)，或者以重复或随机的方式包括它们。作为这样的例子，接头可以包括由SEQ ID NO：11代表的肽接头和由SEQ ID NO：12代表的肽接头中的至少一个，或者可以包括氨基酸序列(GGGGS)N(N是1或更大的整数，优选1至20的整数)(参见下表5)，由此增加了活性物质在细胞中的稳定性并进一步提高了其生产率。
表5
SEQ ID NO 序列信息
SEQ ID NO:11 GS
SEQ ID NO:12 GGG
另外，作为本发明中的接头的实例，由血液最丰富的人白蛋白的282至314位的33个氨基酸，更优选位于292至304位的13个氨基酸组成的肽接头可能会被提及。当从三维结构观察时，该部分大部分暴露于外部，因此使在体内引起免疫应答的可能性最小。然而，本发明不限于此。
另外，在本发明中，在接头和Fc区分开表达然后彼此结合的情况下，接头可以是本领域已知的交联剂。交联剂可以是，例如，1,1-双(重氮乙酰基)-2-苯基乙烷、戊二醛、N-羟基琥珀酰亚胺酯，例如4-叠氮基水杨酸、亚氨酸酯，包括二琥珀酰亚胺酯，例如3,3'-二硫代双(琥珀酰亚胺基丙酸酯)，以及双功能马来酰亚胺，例如双-N-马来酰亚胺基-1,8-辛烷。然而，交联剂不限于此。
作为本发明提供的融合蛋白的一个例子，可能提及包含SEQ ID NO：1表示的Lrig-1蛋白的细胞外结构域，SEQ ID NO：7至10中任一项所代表的铰链区以及由SEQ ID NO：5表示的人IgG1-源的重链恒定区2(CH2)和重链恒定区3(CH3)的融合蛋白(参见下表6)。下表6中的SEQ ID NO：13至16所代表的融合蛋白可以由下表7中的SEQ ID NO：17至20所代表的核酸序列编码(参见下表7)。
表6
Figure BDA0002742983620000081
Figure BDA0002742983620000091
Figure BDA0002742983620000101
Figure BDA0002742983620000111
表7
Figure BDA0002742983620000112
Figure BDA0002742983620000121
Figure BDA0002742983620000131
Figure BDA0002742983620000141
作为本发明提供的融合蛋白的一个例子，可能提及包含SEQ ID NO：3表示的Lrig-1蛋白的细胞外结构域，SEQ ID NO：7至10中任一项所代表的铰链区以及由SEQ ID NO：5表示的人IgG1-源的重链恒定区2(CH2)和重链恒定区3(CH3)的融合蛋白(参见下表8)。下表8中的SEQ ID NO：21至24所代表的融合蛋白可以由下表9中的SEQ ID NO：25至28所代表的核酸序列编码(参见下表7)。
表8
Figure BDA0002742983620000142
Figure BDA0002742983620000151
Figure BDA0002742983620000161
Figure BDA0002742983620000171
表9
Figure BDA0002742983620000172
Figure BDA0002742983620000181
Figure BDA0002742983620000191
Figure BDA0002742983620000201
作为本发明提供的融合蛋白的另一个例子，可能提及包含SEQ ID NO：3表示的Lrig-1蛋白的细胞外结构域，SEQ ID NO：7至10中任一项所代表的铰链区以及由SEQ IDNO：6表示的小鼠IgG2-源的重链恒定区2(CH2)和重链恒定区3(CH3)的融合蛋白(参见下表10)。下表10中的SEQ ID NO：29至32所代表的融合蛋白可以由下表11中的SEQ ID NO：33至36所代表的核酸序列编码(参见下表11)。
表10
Figure BDA0002742983620000202
Figure BDA0002742983620000211
Figure BDA0002742983620000221
表11
Figure BDA0002742983620000231
Figure BDA0002742983620000241
作为本发明提供的融合蛋白的又一个例子，可能提及包含SEQ ID NO：1表示的Lrig-1蛋白的细胞外结构域，SEQ ID NO：11表示的接头，SEQ ID NO：7至10中任一项所代表的铰链区以及由SEQ ID NO：5表示的人IgG1-源的重链恒定区2(CH2)和重链恒定区3(CH3)的融合蛋白(参见下表12)。下表12中的SEQ ID NO：37至40所代表的融合蛋白可以由下表13中的SEQ ID NO：41至44所代表的核酸序列编码(参见下表13)。
表12
Figure BDA0002742983620000251
Figure BDA0002742983620000261
Figure BDA0002742983620000271
表13
Figure BDA0002742983620000281
Figure BDA0002742983620000291
Figure BDA0002742983620000301
作为本发明提供的融合蛋白的又一个例子，可能提及包含SEQ ID NO：3表示的Lrig-1蛋白的细胞外结构域，SEQ ID NO：11表示的接头，SEQ ID NO：7至10中任一项所代表的铰链区以及由SEQ ID NO：5表示的人IgG1-源的重链恒定区2(CH2)和重链恒定区3(CH3)的融合蛋白(参见下表14)。下表14中的SEQ ID NO：45-48所代表的融合蛋白可以由下表15中的SEQ ID NO：49-52所代表的核酸序列编码(参见下表15)。
表14
Figure BDA0002742983620000311
Figure BDA0002742983620000321
Figure BDA0002742983620000331
表15
Figure BDA0002742983620000341
Figure BDA0002742983620000351
Figure BDA0002742983620000361
作为本发明提供的融合蛋白的又一个例子，可能提及包含SEQ ID NO：3表示的Lrig-1蛋白的细胞外结构域，SEQ ID NO：11表示的接头，SEQ ID NO：7至10中任一项所代表的铰链区以及由SEQ ID NO：6表示的小鼠IgG2-源的重链恒定区2(CH2)和重链恒定区3(CH3)的融合蛋白(参见下表16)。下表16中的SEQ ID NO：53-56所代表的融合蛋白可以由下表17中的SEQ ID NO：57-60所代表的核酸序列编码(参见下表17)。
表16
Figure BDA0002742983620000371
Figure BDA0002742983620000381
Figure BDA0002742983620000391
表17
Figure BDA0002742983620000401
Figure BDA0002742983620000411
Figure BDA0002742983620000421
作为本发明提供的融合蛋白的又一个例子，可能提及包含SEQ ID NO：1表示的Lrig-1蛋白的细胞外结构域，SEQ ID NO：12表示的接头，SEQ ID NO：11表示的接头，SEQ IDNO：7至10中任一项所代表的铰链区以及由SEQ ID NO：5表示的人IgG1-源的重链恒定区2(CH2)和重链恒定区3(CH3)的融合蛋白(参见下表18)。下表18中的SEQ ID NO：61-64所代表的融合蛋白可以由下表19中的SEQ ID NO：65-68所代表的核酸序列编码(参见下表19)。
表18
Figure BDA0002742983620000422
Figure BDA0002742983620000431
Figure BDA0002742983620000441
表19
Figure BDA0002742983620000451
Figure BDA0002742983620000461
Figure BDA0002742983620000471
作为本发明提供的融合蛋白的又一个例子，可能提及包含SEQ ID NO：3表示的Lrig-1蛋白的细胞外结构域，SEQ ID NO：12表示的接头，SEQ ID NO：11表示的接头，SEQ IDNO：7至10中任一项所代表的铰链区以及由SEQ ID NO：5表示的人IgG1-源的重链恒定区2(CH2)和重链恒定区3(CH3)的融合蛋白(参见下表20)。下表20中的SEQ ID NO：69-72所代表的融合蛋白可以由下表21中的SEQ ID NO：73-76所代表的核酸序列编码(参见下表21)。
表20
Figure BDA0002742983620000481
Figure BDA0002742983620000491
Figure BDA0002742983620000501
表21
Figure BDA0002742983620000511
Figure BDA0002742983620000521
Figure BDA0002742983620000531
作为本发明提供的融合蛋白的又一个例子，可能提及包含SEQ ID NO：3表示的Lrig-1蛋白的细胞外结构域，SEQ ID NO：12表示的接头，SEQ ID NO：11表示的接头，SEQ IDNO：7至10中任一项所代表的铰链区以及由SEQ ID NO：6表示的小鼠IgG2-源的重链恒定区2(CH2)和重链恒定区3(CH3)的融合蛋白(参见下表22)。下表22中的SEQ ID NO：77-80所代表的融合蛋白可以由下表23中的SEQ ID NO：81-84所代表的核酸序列编码(参见下表23)。
表22
Figure BDA0002742983620000541
Figure BDA0002742983620000551
Figure BDA0002742983620000561
表23
Figure BDA0002742983620000571
Figure BDA0002742983620000581
Figure BDA0002742983620000591
本发明提供的融合蛋白可以与效应T细胞上存在的Lrig-1蛋白的配体相互作用，从而抑制它们的表面上具有Lrig-1蛋白的调节性T细胞(Treg细胞)与效应T细胞之间的相互作用，以使调节性T细胞的活性被抑制并且效应T细胞的活性被维持或升高，从而有效地抑制癌细胞，特别是实体癌细胞的生长。
根据本发明的另一个实施方式，提供了一种核酸分子，其编码本发明提供的融合蛋白。
本发明的核酸分子包括本领域技术人员已知的通过使本发明提供的融合蛋白的氨基酸序列翻译成多核苷酸序列而获得的任何核酸分子。因此，由于开放阅读框(ORF)，可以制备各种多核苷酸序列，所有这些也都包括在本发明的核酸分子中。
作为本发明的优选实例，所述核酸分子可以但不限于由SEQ ID NO：17-20、25-28、33-36、41-44、49-52、57-60、65-68、73-76和81-84中的任何一个表示。
根据本发明的又一实施方式，提供一种表达载体，其中插入本发明中提供的分离的核酸分子。
在本发明中，“载体”是能够转运与其连接的另一核酸的核酸分子。一种载体是“质粒”，其是指环状双链DNA，其中可以连接其他DNA区段。另一种载体是噬菌体载体。还有一种载体是病毒载体，其中另外的DNA区段可以连接到病毒基因组中。某些载体能够在引入它们的宿主细胞中自主复制(例如，具有细菌复制起点的细菌载体是游离型哺乳动物载体)。在导入宿主细胞后，可以将其他载体(例如，非游离型哺乳动物载体)整合到宿主细胞的基因组中，并因此与宿主基因组一起复制。另外，某些载体能够指导与其可操作连接的基因的表达。此类载体在本文中称为“重组表达载体”或简称为“表达载体”。通常，可用于重组DNA技术的表达载体通常为质粒形式。在本说明书中，“质粒”和“载体”可以互换使用，因为质粒是最常用的载体形式。
本发明中表达载体的具体实例可以选自但不限于下组：商业上广泛使用的pCDNA载体、F、R1、RP1、Col、pBR322、ToL、Ti载体；粘粒；噬菌体例如λ、λ形的、M13、Mu、p1、P22、Qμ、T-even、T2、T3、T7；植物病毒。对于本领域技术人员而言，任何已知的表达载体均可在本发明中用作表达载体，并且根据靶宿主细胞的性质选择表达载体。可通过磷酸钙转染、病毒感染、DEAE-葡萄聚糖介导的转染、脂质体转染或电穿孔来将载体引入宿主细胞。然而，本发明不限于此，本领域技术人员可以采用并使用适合于所使用的表达载体和宿主细胞的导入方法。可通过磷酸钙转染、病毒感染、DEAE-葡萄聚糖介导的转染、脂质体转染或电穿孔来将载体引入宿主细胞。然而，本发明不限于此，本领域技术人员可以采用和使用适合于所使用的表达载体和宿主细胞的导入方法。载体可优选包含至少一种选择标记。然而，本发明不限于此，并且取决于是否产生产物，可以使用不包含选择标记的载体来进行选择。根据靶宿主细胞选取选择标记，这是使用本领域技术人员已知的方法完成的，因此本发明对此没有限制。
为了促进本发明的核酸分子的纯化，可以将标签序列插入表达载体并与之融合。标签包括但不限于六组氨酸标签、血凝素标签、myc标签或标志标签，并且本领域技术人员已知的有助于纯化的任何标签都可以用于本发明。
根据本发明的又一个实施方式，提供了用本发明提供的表达载体转染的宿主细胞系。
在本发明中，“宿主细胞”包括单个细胞或细胞培养物，其可以是或已经是用于掺入多肽插入物的载体的接受者。宿主细胞包括单个宿主细胞的后代，并且由于自然、偶然或有意的突变，该后代不一定与原始亲本细胞完全相同(在形态或基因组DNA补体上)。宿主细胞包括用本文的多核苷酸体内转染的细胞。
在本发明中，宿主细胞可以包括哺乳动物、植物、昆虫、真菌或细胞来源的细胞，并且可以是例如细菌细胞，如大肠杆菌、链霉菌、鼠伤寒沙门氏菌；真菌细胞，例如酵母细胞和巴斯德毕赤酵母；昆虫细胞，如果蝇和草地贪夜蛾Sf9细胞；动物细胞，例如CHO(中国仓鼠卵巢)细胞、SP2/0(小鼠骨髓瘤)、人类淋巴母细胞、COS、NSO(小鼠骨髓瘤)、293T、Bowes黑色素瘤细胞、HT-1080、小仓鼠肾(BHK)细胞、人类胚胎肾(HEK)细胞或PERC.6(人类视网膜细胞)；或植物细胞。然而，宿主细胞不限于此，并且可以用作宿主细胞系的本领域技术人员已知的任何细胞都是可用的。
根据本发明的又一个实施方式，提供了一种预防或治疗癌症的药物组合物，其包含本发明提供的融合蛋白作为活性成分。
如本文所用，术语“癌症”是指或指示以哺乳动物细胞的生长为特征的生理状况，其没有以典型方式被调节。本发明中待预防、改善或治疗的癌症可以是由实体器官中细胞异常生长引起的聚结物形成的实体瘤，并且可以是但不限于胃癌、肝癌、胶质细胞瘤、卵巢癌、结肠直肠癌、头颈癌、膀胱癌、肾细胞癌、乳腺癌、转移癌、前列腺癌、胰腺癌、黑色素瘤、肺癌等，取决于实体器官的位置，其中优选黑色素瘤或结肠直肠癌。
同时，在本发明中，“预防”可包括但不限于使用本发明的药物组合物阻断疾病症状或抑制或延迟症状的任何行为。
此外，在本发明中，“治疗”可以包括但不限于使用本发明的药物组合物来改善或有益地改变疾病症状的任何行为。
在本发明中，药物组合物的特征可以在于是胶囊、片剂、颗粒剂、注射剂、软膏剂、散剂或饮料的形式，并且药物组合物的特征是可以针对人。
在本发明中，药物组合物可以按照常规方法分别制成口服制剂的形式，例如散剂，颗粒剂，胶囊剂，片剂和水性混悬剂，外用制剂，栓剂和无菌注射液并被使用。但是，药物组合物不限于此。本发明的药物组合物可以进一步包含药学上可接受的载体。作为药学上可接受的载体，可以将粘合剂、助流剂、崩解剂、赋形剂、增溶剂、分散剂、稳定剂、助悬剂、颜料、调味剂等用于口服给药；可以将缓冲剂、防腐剂、止痛剂、增溶剂、等渗剂、稳定剂等混合用于注射剂；可以将碱、赋形剂、润滑剂、防腐剂等用于局部给药。本发明的药物组合物的制剂可以通过以各种方式与如上所述的药学上可接受的载体混合制备。例如，对于口服给药，可以将药物组合物制成片剂、锭剂、胶囊剂、酏剂、混悬剂、糖浆剂、植入剂(wafer)等形式。对于注射，药物组合物可以配制成单位剂量安瓿或多种剂量的形式。或者，可以将药物组合物配制成溶液、悬浮液、片剂、胶囊、缓释制剂等。
同时，作为适合于制备制剂的载体、稀释剂或赋形剂的实例，可以使用乳糖、右旋糖、蔗糖、山梨糖醇、甘露醇、木糖醇、赤藓糖醇、麦芽糖醇、淀粉、阿拉伯胶、藻酸盐、明胶、磷酸钙、硅酸钙、纤维素、甲基纤维素、微晶纤维素、聚乙烯吡咯烷酮、水、羟基苯甲酸甲酯、羟基苯甲酸丙酯、滑石粉、硬脂酸镁、矿物油等。另外，可以进一步包括填充剂、抗凝剂、润滑剂、湿润剂、香料、乳化剂、防腐剂等。
在本发明中，药物组合物的给药途径包括但不限于口服、静脉内、肌内、动脉内、髓内、硬膜内、心内、透皮、皮下、腹膜内、鼻内、肠、局部、舌下或直肠途径。口服或肠胃外给药是优选的。
在本发明中，“肠胃外”包括皮下、皮内、静脉内、肌肉内、关节内、囊内、胸骨内、硬膜内、病灶内和颅内注射或输注技术。本发明的药物组合物也可以栓剂的形式用于直肠给药。
本发明的药物组合物可根据多种因素而广泛变化，包括所用某种化合物的活性、患者的年龄、体重、总体健康状况、性别、饮食、给药时间、给药途径、排泄率、药物组合以及要预防或治疗的某些疾病的严重性。药物组合物的剂量可以根据患者的状况、体重、疾病的严重程度、药物形式、给药途径和持续时间而变化，并且可以由本领域技术人员适当地选择。药物组合物可以每天0.0001至50mg/kg或0.001至50mg/kg的量施用。给药可以一天一次或一天几次。该剂量无意以任何方式限制本发明的范围。本发明的药物组合物可以配制成丸剂、糖衣片剂、胶囊剂、液体剂、凝胶剂、糖浆剂、浆剂或混悬剂的形式。
根据本发明的又一个实施方式，提供了一种预防或治疗癌症的方法，其包括对个体施用根据本发明的融合蛋白或包含该融合蛋白的组合物的步骤。
本发明的融合蛋白可与存在于效应T细胞上的Lrig-1蛋白的配体相互作用，以抑制在其上具有Lrig-1蛋白的调节性T细胞(Treg细胞)与效应T细胞之间的相互作用，使得调节性T细胞的活性被抑制并且效应T细胞的活性得以维持或升高，从而有效地抑制了癌细胞特别是实体癌细胞的生长。
在本发明中，“个体”是疑似患有癌症的个体，并且疑似患有癌症的个体是指已经发展或可能发展所述疾病的哺乳动物，例如人，小鼠和家畜。但是，其中包括可以用本发明的融合蛋白或包含该融合蛋白的组合物治疗的任何个体而没有限制。
本发明的方法可以包括以药学有效量施用融合蛋白或包含该融合蛋白的组合物。主治医师或兽医可以在合理的医学判断范围内确定适当的每日总用量，并可以进行一次或多次给药。然而，出于本发明的目的，针对特定患者的特定治疗有效量优选根据各种因素进行不同的应用，包括要实现的反应的类型和程度，是否与其他试剂一起使用的特定组合物，视情况可以是患者的年龄，体重，总体健康状况，性别和饮食，给药时间，给药途径，组合物的分泌率，治疗时间以及与特定组合物同时使用或与联合使用的药物，以及在医学领域众所周知的类似因素。
同时，用于预防或治疗癌症的方法可以是但不限于联合疗法，其进一步包括给予对一种或多种癌症疾病具有治疗活性的化合物或物质。
在本发明中，“联合”应理解为代表同时、单独或顺序给药。在以顺序或单独的方式进行给药的情况下，第二组分应以一定的间隔给药，以使联合的有益作用不会丧失。
在本发明中，融合蛋白的剂量可以是但不限于每千克患者体重约0.0001微克至500毫克。
发明的有益效果
本发明提供的融合蛋白可以与效应T细胞上存在的Lrig-1蛋白的配体相互作用，以抑制在其表面上具有Lrig-1蛋白的调节性T细胞(Treg细胞)与效应T细胞之间的相互作用，以致调节性T细胞的活性被抑制并且效应T细胞的活性被维持或升高，从而有效地抑制癌细胞特别是实体癌细胞的生长。
附图说明
图1示出根据本发明一实施方式的Lrig-1蛋白的结构。
图2示出根据本发明一实施方式的Lrig-1蛋白的结构。
图3示出根据本发明一实施方式的Lrig-1蛋白的表位的预测结果。
图4示出根据本发明一实施方式的Lrig-1蛋白的表位的预测结果。
图5示出根据本发明一实施方式的Lrig-1mRNA的表达水平。
图6示出根据本发明一实施方式的Lrig-1mRNA的表达水平。
图7示出根据本发明一实施方式的Lrig-1mRNA的表达水平。
图8示出根据本发明一实施方式的Lrig-1、Lrig-2和Lrig-3mRNA的表达水平。
图9示出根据本发明一实施方式通过比较调节性T细胞和非调节性T细胞中Lrig-1蛋白的表达水平获得的结果。
图10示出根据本发明一实施方式的调节性T细胞的表面上Lrig-1蛋白的表达。
图11示出根据本发明一实施方式的融合蛋白所表现出抑制调节性T细胞对效应T细胞的抑制活性的效果。
图12示出其中存在根据本发明一实施方式的融合蛋白识别的配体的T细胞的子集。
图13示出使用根据本发明一实施方式的融合蛋白的癌症治疗实验的实验设计。
图14示出通过分析由根据本发明一实施方式的融合蛋白实现的癌症治疗效果而获得的结果。
图15示出通过分析由根据本发明一实施方式的融合蛋白实现的癌症治疗效果而获得的结果。
具体实施方式
根据本发明的一实施方式，提供一种融合蛋白，其包含富含亮氨酸和免疫球蛋白样结构域1(Lrig-1)蛋白的细胞外结构域和免疫球蛋白Fc区。
作为本发明的一实例，免疫球蛋白Fc区可以是IgG-、IgA-、IgM-、IgD-或IgE-源的Fc区，或可以包括IgG-、IgA-、IgM-、IgD-或IgE-源的重链恒定区2(CH2)和重链恒定区3(CH3)。然而，本发明不限于此。
作为本发明的一个实例，免疫球蛋白Fc区可包括IgG-、IgA-、IgM-、IgD-、IgE-或阿巴西普-源的铰链区。然而，本发明不限于此。
在本发明中，Lrig-1蛋白的细胞外结构域可以通过接头连接至免疫球蛋白Fc区的N端或C端。然而，本发明不限于此。
根据本发明的另一实施方式，提供了用于预防或治疗癌症的药物组合物，其包含作为活性成分的本发明的融合蛋白。
本发明提供的融合蛋白可以与效应T细胞上存在的Lrig-1蛋白的配体相互作用，以抑制在其表面上具有Lrig-1蛋白的调节性T细胞(Treg细胞)与效应T细胞之间的相互作用，以致调节性T细胞的活性被抑制并且效应T细胞的活性被维持或升高，从而有效地抑制癌细胞特别是实体癌细胞的生长。
实施方式
在下文中，将通过实施例更详细地描述本发明。这些实施例仅用于更详细地描述本发明，并且对于本领域技术人员而言将显而易见的是，根据本发明的主旨，本发明的范围不受这些实施例的限制。
实施例
[制备实施例1]T细胞亚型细胞培养
为了鉴定Lrig-1蛋白是否仅在调节性T细胞(Treg)中表达，制备了T细胞亚群Th0、Th1、Th2、Th17和iTreg。与天然分离的nTreg不同，iTreg是指在具有以下组成的培养基中人工诱导分化的细胞。
通过首先分离从小鼠脾脏获得的原初T细胞，形成含有10％胎牛血清(FBS；HyClone，犹他州洛根)的RPMI1640(英杰Gibco，格兰德岛，纽约)营养培养基，进一步包含下表24中的各个成分，并在37℃，5％CO2的培养箱中进行72小时培养，从而诱导T细胞亚群分化为相应的细胞。
表24
分化细胞 组成
Th0 抗-CD3、抗-CD28
Th1 IL-12、抗-IL-4抗体
Th2 IL-4、抗-IFNβ
Th17 IL-6、TGFβ、抗-IFNβ、抗-IL-4
iTreg IL-2、TGFβ
[实施例1]Lrig-1的结构分析
预测Lrig-1蛋白的细胞外结构域的三维空间结构以产生包含Lrig-1蛋白的细胞外结构域的融合蛋白，Lrig-1蛋白是调节性T细胞的表面蛋白。
首先，为了预测表位的碱基序列，使用了Uniprot(http://www.uniprot.org)和RCSB蛋白数据库(http://www.rcsb.org/pdb)的工具来预测Lrig-1蛋白的细胞外结构域(ECD)的三维空间结构，以便鉴别ECD的结构。然后，结果在图1和2中示出。
如图1所示，在Lrig-1蛋白的细胞外结构域的Lrig-LRR结构域(第41-494位的氨基酸序列)中总共存在LRR1至LRR15 15个富含亮氨酸的区域。每个LRR结构域由23-27个氨基酸组成，其中存在3至5个亮氨酸。
另外，如图2所示，在Lrig-1蛋白的细胞外结构域中的Lrig-1蛋白的494-781位的氨基酸序列中存在三个免疫球蛋白样结构域。
[实施例2]Lrig-1表位氨基酸序列的预测
使用Ellipro服务器(http://tools.iedb.org/ellipro/)进行上述碱基序列的预测，该服务器是基于Lrig-1蛋白结构的表位预测软件。之所以使用Ellipro搜索引擎，是因为它对应于已知的用于预测表位的现有算法中最可靠的搜索引擎。
将实施例1中分析的细胞外结构域输入到表位预测软件中，然后在图3和4中示出预测的表位的预测的连续或不连续氨基酸序列。
如图3和4所示，预测了总共22个连续的表位氨基酸序列，并且预测了总共8个不连续的表位氨基酸序列。
[实施例3]在调节性T细胞中Lrig-1mRNA的特异性表达的鉴定
验证了Lrig-1蛋白是否可以作为调节性T细胞的特异性生物标志物。
为了进行验证，使用磁激活细胞分选(MACS)通过CD4珠子从小鼠脾脏中分离出CD4+T细胞。随后，使用CD25抗体用荧光激活细胞分选仪(FACS)分离调节性T(CD4+CD25+ T)细胞和非调节性T(CD4+CD25- T)细胞。对于各个细胞和在制备实施例1中分化的细胞，使用Trizol提取mRNA，然后根据制造商提供的方案使用gDNA提取试剂盒(凯杰)从基因组RNA中去除gDNA。通过BDsprint cDNA合成试剂盒(克隆技术)将去除gDNA的mRNA合成为cDNA。
进行实时聚合酶链反应(RT PCR)以定量鉴定cDNA中Lrig-1mRNA的表达水平。
使用SYBR Green(分子探针)，按照制造商提供的规程，使用下表25中所示的引物进行实时聚合酶链反应，条件为：40个循环，包括95℃3分钟，61℃15秒，72℃30秒，使用ΔCT方法计算相对基因表达水平，并使用HPRT进行归一化。结果在图5-8中示出。
表25
Figure BDA0002742983620000651
如图5所示，可以看出Lrig-1在调节性T(CD4+CD25+ T)细胞中的表达比非调节性T(CD4+CD25- T)细胞高18.1倍。这是比以前已知的调节性T细胞标记Lag3和Ikzf4高约10倍的表达水平。
另外，如图6和7所示，与其他类型的免疫细胞相比，Lrig-1mRNA在调节性T细胞中显著高表达，特别是在自然分离的调节性T细胞(nTreg)中与诱导型调节性T(iTreg)细胞相比显著高。
另外，如图8所示，在对应Lrig家族的Lrig-1、Lrig-2和Lrig-3中，Lrig-1的表达最高。
从以上结果可以看出，根据本发明的Lrig-1蛋白在调节性T细胞，特别是天然存在的调节性T细胞中特异性表达。
[实施例4]Lrig-1蛋白在调节性T细胞中的特异性表达的鉴定
鉴定了从Lrig-1mRNA表达的Lrig-1蛋白是否仅在调节性T细胞中特异性表达。
使用敲入FOXP3-RFP的小鼠，其中通过将红色荧光蛋白(RFP)与FOXP3启动子偶联获得的FOXP3-RFP，FOXP3启动子是调节性T细胞特有的转录因子，使用磁激活细胞分选法(MACS)通过CD4珠子从小鼠的脾脏分离CD4+ T细胞。随后，使用RFP蛋白，通过荧光激活细胞分选仪(FACS)进行分离，从而获得调节性T(CD4+RFP+ T)细胞和非调节性T(CD4+RFP- T)细胞。将各个细胞用购买的Lrig-1抗体染色，并将阴性对照用同型匹配的对照抗体染色，以用荧光激活的细胞分选仪检测Lrig-1的表达水平。结果在图9中示出。
如图9所示，用虚线指示的非调节性T细胞显示出与阴性对照几乎相同的Lrig-1表达水平，而在调节性T细胞中存在大量高表达Lrig-1的细胞。
从以上结果可以看出，根据本发明的Lrig-1蛋白在调节性T细胞中特异性表达。
[实施例5]Lrig-1蛋白在调节性T细胞表面上的特异性表达的鉴定
从成为细胞治疗靶标的角度来看，Lrig-1蛋白必须表达在调节性T细胞的表面，从而可以更有效地进行靶标治疗，鉴定了Lrig-1蛋白是否在调节性T细胞的表面表达。
使用抗CD4-APC和抗Lrig-1-PE抗体对制备实施例1的各个分化的T细胞亚群进行染色，并使用荧光激活的细胞分选仪(FACS)测量各个细胞表面Lrig-1的表达水平。结果在图10中示出。
如图10所示，Lrig-1在活化T细胞、Th1细胞、Th2细胞、Th17细胞和原初T细胞中的表达量为0.77至15.3，而在分化诱导T细胞(iTreg细胞)中Lrig-1的表达量高达83.9。
从以上结果可以看出，根据本发明的Lrig-1蛋白不仅在调节性T(Treg)细胞中特异性表达，而且特别是在Treg细胞表面上以较高水平表达。
[制备实施例]融合蛋白的生产
1.表达载体的构建
为了生产根据本发明的融合蛋白，合成了编码表26所示的各融合蛋白的各核酸序列。将NheI和EcoRI限制酶序列分别添加到核酸序列的5'和3'末端，并在5'末端的限制酶序列之后插入以下序列：Kozak序列(GCCACC)，用于蛋白质翻译的起始密码子，以及小鼠IgGκ轻链信号肽(ATGGAAACCGATACTCTGCTGCTGTGGGTGCTGCTGCTGTGGGGGTGCCAGGCTCTCTACCGGG)，使表达的蛋白质分泌到细胞外。随后，在编码表26所示的各融合蛋白的各核酸序列之后插入终止密码子，其中各融合蛋白包括人源性Lrig-1蛋白的细胞外结构域，任选的接头，铰链区，人IgG1-源的重链恒定区2(CH2)和重链恒定区3(CH3)。使用两个限制性酶序列NheI和EcoRI，将编码本发明融合蛋白的核酸序列克隆到pcDNA3.1(+)表达载体中。
表26
Figure BDA0002742983620000661
Figure BDA0002742983620000671
2.融合蛋白的纯化
使用聚乙烯亚胺，用如在以上编号1中构建的表达载体转化293F细胞，然后在37℃和8％CO2的条件下培养6天。过滤培养上清液，使其通过A蛋白树脂，并用1×PBS洗涤。用pH3.5的0.1M甘氨酸溶液进行洗脱。然后，向所得溶液中加入pH 9.0的1M TRIS溶液以中和pH。随后，将溶液用PBS溶液透析，然后浓缩并使用。
[实施例6]本发明的融合蛋白表现出抑制调节性T细胞对效应T细胞的抑制活性的效果
进行以下实验以鉴定在制备实施例5中生产的(使用SEQ ID NO：25所示的核酸序列生产)，由SEQ ID NO：21代表的根据本发明的融合蛋白是否与Lrig-1蛋白的配体结合，以致抑制配体与调节性T细胞之间的相互作用，从而最终降低了调节性T细胞对效应T细胞增殖的抑制活性。具体地，在调节性T细胞和效应T细胞共培养的条件下添加制备实施例5的融合蛋白，以鉴定调节性T细胞对效应T细胞增殖的抑制活性的变化。结果在图11中示出。
如图11所示，发现用本发明的融合蛋白处理导致调节性T细胞对效应T细胞增殖的抑制活性降低。
从这些结果可以看出，根据本发明的融合蛋白与Lrig-1蛋白的配体相互作用，以抑制表面上具有Lrig-1蛋白的调节性T细胞与配体之间的相互作用，从而使调节性T细胞的活性受到抑制，效应T细胞的活性得以维持或提高。
[实施例7]本发明的融合蛋白识别的Lrig-1配体的分布的鉴定
如实施例6所示，可以看出在制备实施例5中生产的(使用SEQ ID NO：25所示的核酸序列生产)，由SEQ ID NO：21代表的根据本发明的融合蛋白能够识别Lrig-1配体。因此，为了发现其中存在Lrig-1配体的免疫细胞，诱导被认为是调节性T细胞靶标的原初T细胞分化为活化的T细胞、Th1细胞、Th2细胞或Th17细胞，然后将这些分化的细胞用制备实施例5的融合蛋白(一抗)和抗人PE抗体(二抗)染色。使用荧光激活细胞分选仪(FACS)测量各个细胞表面融合蛋白的表达水平。结果在图12中示出。
如图12所示，根据本发明的融合蛋白几乎不染色原初T细胞表面上的抗原(0.71％)，而染色活化的T细胞、Th1细胞、Th2细胞和Th17细胞表面上96％或更多的抗原。
从这些结果，发现Lrig-1配体是通过T细胞受体刺激而诱导的表面蛋白。
[实施例8]根据本发明的融合蛋白的癌症治疗作用
为了鉴定在制备实施例5中生产的(使用SEQ ID NO：25所示的核酸序列生产)，由SEQ ID NO：21代表的根据本发明的融合蛋白对实体肿瘤的治疗作用，如图13所示，将B16F10黑素瘤细胞以3×105个细胞的量皮下注射到小鼠的背部区域中，然后在第4、8和12天以200微克的量腹腔内注射制备实施例5的融合蛋白。在移植黑素瘤细胞后，测量肿瘤体积随时间的变化，结果示于图14中。
如图14所示，与未进行治疗的阴性对照相比，在用本发明的融合蛋白进行治疗的情况下，观察到黑素瘤的肿瘤大小显著减少。
[实施例9]根据本发明的融合蛋白的癌症治疗作用
为了鉴定在制备实施例34中生产的(使用SEQ ID NO：82所示的核酸序列生产)，由SEQ ID NO：78代表的根据本发明的融合蛋白对实体肿瘤的治疗作用，将CT-26结肠直肠癌细胞以3×105个细胞的量皮下注射到小鼠的背部区域，然后在第4、8和12天以200微克的量腹腔内注射制备实施例34的融合蛋白。在移植结肠直肠癌细胞后，测量肿瘤体积随时间的变化，结果示于图15中。
如图15所示，与未进行治疗的阴性对照相比，在用本发明的融合蛋白进行治疗的情况下，观察到结肠直肠瘤的肿瘤大小显著减少。
从这些结果可以看出，根据本发明，包含Lrig-1蛋白的细胞外结构域和免疫球蛋白Fc区的融合蛋白能够抑制调节性T细胞与效应T细胞之间的相互作用，以致调节性T细胞的活性被抑制，效应T细胞的活性得以维持或提高，从而有效地抑制癌细胞特别是实体癌细胞的生长。
尽管上面已经详细描述了本发明，但是本发明的范围不限于此。对于本领域技术人员将显而易见的是，在不脱离权利要求中描述的本发明的技术精神的情况下，可以进行各种修改和改变。
工业可用性
本发明涉及一种新型融合蛋白，其包含Lrig-1蛋白的细胞外结构域和免疫球蛋白Fc区，并且涉及其在预防或治疗癌症中的用途。
序列表
<110> 古德T细胞有限公司
<120> 新型融合蛋白和用于预防或治疗癌症的包含该融合蛋白的药物组合物
<130> PDPB187151k01
<150> KR 10-2018-0048343
<151> 2018-04-26
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gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
2280
<210> 5
<211> 206
<212> PRT
<213> 智人(Homo sapiens)
<400> 5
Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
1 5 10 15
Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
20 25 30
Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
35 40 45
Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
50 55 60
Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
65 70 75 80
Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
85 90 95
Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
100 105 110
Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
115 120 125
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
130 135 140
Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
145 150 155 160
Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
165 170 175
Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
180 185 190
Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
195 200 205
<210> 6
<211> 206
<212> PRT
<213> 小家鼠(Mus musculus)
<400> 6
Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile Ser Leu Ser Pro
1 5 10 15
Ile Val Thr Cys Val Val Val Asp Val Ser Glu Asp Asp Pro Asp Val
20 25 30
Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His Thr Ala Gln Thr
35 40 45
Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu Arg Val Val Ser Ala
50 55 60
Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys Glu Phe Lys Cys
65 70 75 80
Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu Arg Thr Ile Ser
85 90 95
Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val Tyr Val Leu Pro Pro
100 105 110
Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr Leu Thr Cys Met Val
115 120 125
Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu Trp Thr Asn Asn Gly
130 135 140
Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val Leu Asp Ser Asp
145 150 155 160
Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu Lys Lys Asn Trp
165 170 175
Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val His Glu Gly Leu His
180 185 190
Asn His His Thr Thr Lys Ser Phe Ser Arg Thr Pro Gly Lys
195 200 205
<210> 7
<211> 26
<212> PRT
<213> 智人(Homo sapiens)
<400> 7
Glu Pro Lys Ser Ser Asp Lys Thr His Thr Ser Pro Pro Cys Pro Ala
1 5 10 15
Pro Glu Leu Leu Gly Gly Pro Ser Val Phe
20 25
<210> 8
<211> 27
<212> PRT
<213> 小家鼠(Mus musculus)
<400> 8
Glu Pro Arg Gly Pro Thr Ile Lys Pro Cys Pro Pro Cys Lys Cys Pro
1 5 10 15
Ala Pro Asn Leu Leu Gly Gly Pro Ser Val Phe
20 25
<210> 9
<211> 39
<212> PRT
<213> 智人(Homo sapiens)
<400> 9
Arg Asn Thr Gly Arg Gly Gly Glu Glu Lys Lys Lys Glu Lys Glu Lys
1 5 10 15
Glu Glu Gln Glu Glu Arg Glu Thr Lys Thr Pro Glu Cys Pro Ser His
20 25 30
Thr Gln Pro Leu Gly Val Phe
35
<210> 10
<211> 26
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 10
Glu Pro Lys Ser Ser Asp Lys Thr His Thr Ser Pro Pro Ser Pro Ala
1 5 10 15
Pro Glu Leu Leu Gly Gly Ser Ser Val Phe
20 25
<210> 11
<211> 2
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 11
Gly Ser
1
<210> 12
<211> 3
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 12
Gly Gly Gly
1
<210> 13
<211> 994
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 13
Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala Gly Asp
1 5 10 15
Ser Leu Asp Cys Gly Gly Arg Gly Leu Ala Ala Leu Pro Gly Asp Leu
20 25 30
Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Lys Leu Ser Glu
35 40 45
Ile Asp Pro Ala Gly Phe Glu Asp Leu Pro Asn Leu Gln Glu Val Tyr
50 55 60
Leu Asn Asn Asn Glu Leu Thr Ala Val Pro Ser Leu Gly Ala Ala Ser
65 70 75 80
Ser His Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Arg Ser Val
85 90 95
Glu Gly Ser Gln Leu Lys Ala Tyr Leu Ser Leu Glu Val Leu Asp Leu
100 105 110
Ser Leu Asn Asn Ile Thr Glu Val Arg Asn Thr Cys Phe Pro His Gly
115 120 125
Pro Pro Ile Lys Glu Leu Asn Leu Ala Gly Asn Arg Ile Gly Thr Leu
130 135 140
Glu Leu Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr Leu Arg
145 150 155 160
Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Arg Ala Phe Lys Leu
165 170 175
Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg Leu Ile
180 185 190
Glu Gly Leu Thr Phe Gln Gly Leu Asn Ser Leu Glu Val Leu Lys Leu
195 200 205
Gln Arg Asn Asn Ile Ser Lys Leu Thr Asp Gly Ala Phe Trp Gly Leu
210 215 220
Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val Glu Val
225 230 235 240
Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu His Leu
245 250 255
Ser Asn Asn Ser Ile Ala Arg Ile His Arg Lys Gly Trp Ser Phe Cys
260 265 270
Gln Lys Leu His Glu Leu Val Leu Ser Phe Asn Asn Leu Thr Arg Leu
275 280 285
Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Val Leu Arg Leu
290 295 300
Ser His Asn Ser Ile Ser His Ile Ala Glu Gly Ala Phe Lys Gly Leu
305 310 315 320
Arg Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser Gly Thr
325 330 335
Ile Glu Asp Thr Ser Gly Ala Phe Ser Gly Leu Asp Ser Leu Ser Lys
340 345 350
Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg Ala Phe
355 360 365
Ser Gly Leu Glu Gly Leu Glu His Leu Asn Leu Gly Gly Asn Ala Ile
370 375 380
Arg Ser Val Gln Phe Asp Ala Phe Val Lys Met Lys Asn Leu Lys Glu
385 390 395 400
Leu His Ile Ser Ser Asp Ser Phe Leu Cys Asp Cys Gln Leu Lys Trp
405 410 415
Leu Pro Pro Trp Leu Ile Gly Arg Met Leu Gln Ala Phe Val Thr Ala
420 425 430
Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe Ser Val
435 440 445
Pro Pro Glu Ser Phe Val Cys Asp Asp Phe Leu Lys Pro Gln Ile Ile
450 455 460
Thr Gln Pro Glu Thr Thr Met Ala Met Val Gly Lys Asp Ile Arg Phe
465 470 475 480
Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe Ala Trp
485 490 495
Lys Lys Asp Asn Glu Val Leu Thr Asn Ala Asp Met Glu Asn Phe Val
500 505 510
His Val His Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr Ile Leu
515 520 525
His Leu Arg Gln Val Thr Phe Gly His Glu Gly Arg Tyr Gln Cys Val
530 535 540
Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg Leu Thr
545 550 555 560
Val Asn Val Leu Pro Ser Phe Thr Lys Thr Pro His Asp Ile Thr Ile
565 570 575
Arg Thr Thr Thr Val Ala Arg Leu Glu Cys Ala Ala Thr Gly His Pro
580 585 590
Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe Pro Ala
595 600 605
Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val Phe Phe
610 615 620
Ile Thr Asp Val Lys Ile Asp Asp Ala Gly Val Tyr Ser Cys Thr Ala
625 630 635 640
Gln Asn Ser Ala Gly Ser Ile Ser Ala Asn Ala Thr Leu Thr Val Leu
645 650 655
Glu Thr Pro Ser Leu Val Val Pro Leu Glu Asp Arg Val Val Ser Val
660 665 670
Gly Glu Thr Val Ala Leu Gln Cys Lys Ala Thr Gly Asn Pro Pro Pro
675 680 685
Arg Ile Thr Trp Phe Lys Gly Asp Arg Pro Leu Ser Leu Thr Glu Arg
690 695 700
His His Leu Thr Pro Asp Asn Gln Leu Leu Val Val Gln Asn Val Val
705 710 715 720
Ala Glu Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Thr Leu Gly
725 730 735
Thr Glu Arg Ala His Ser Gln Leu Ser Val Leu Pro Ala Ala Gly Cys
740 745 750
Arg Lys Asp Gly Thr Thr Val Gly Ile Phe Glu Pro Lys Ser Ser Asp
755 760 765
Lys Thr His Thr Ser Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly
770 775 780
Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile
785 790 795 800
Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu
805 810 815
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
820 825 830
Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg
835 840 845
Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
850 855 860
Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu
865 870 875 880
Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr
885 890 895
Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu
900 905 910
Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp
915 920 925
Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val
930 935 940
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp
945 950 955 960
Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His
965 970 975
Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro
980 985 990
Gly Lys
<210> 14
<211> 995
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 14
Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala Gly Asp
1 5 10 15
Ser Leu Asp Cys Gly Gly Arg Gly Leu Ala Ala Leu Pro Gly Asp Leu
20 25 30
Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Lys Leu Ser Glu
35 40 45
Ile Asp Pro Ala Gly Phe Glu Asp Leu Pro Asn Leu Gln Glu Val Tyr
50 55 60
Leu Asn Asn Asn Glu Leu Thr Ala Val Pro Ser Leu Gly Ala Ala Ser
65 70 75 80
Ser His Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Arg Ser Val
85 90 95
Glu Gly Ser Gln Leu Lys Ala Tyr Leu Ser Leu Glu Val Leu Asp Leu
100 105 110
Ser Leu Asn Asn Ile Thr Glu Val Arg Asn Thr Cys Phe Pro His Gly
115 120 125
Pro Pro Ile Lys Glu Leu Asn Leu Ala Gly Asn Arg Ile Gly Thr Leu
130 135 140
Glu Leu Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr Leu Arg
145 150 155 160
Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Arg Ala Phe Lys Leu
165 170 175
Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg Leu Ile
180 185 190
Glu Gly Leu Thr Phe Gln Gly Leu Asn Ser Leu Glu Val Leu Lys Leu
195 200 205
Gln Arg Asn Asn Ile Ser Lys Leu Thr Asp Gly Ala Phe Trp Gly Leu
210 215 220
Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val Glu Val
225 230 235 240
Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu His Leu
245 250 255
Ser Asn Asn Ser Ile Ala Arg Ile His Arg Lys Gly Trp Ser Phe Cys
260 265 270
Gln Lys Leu His Glu Leu Val Leu Ser Phe Asn Asn Leu Thr Arg Leu
275 280 285
Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Val Leu Arg Leu
290 295 300
Ser His Asn Ser Ile Ser His Ile Ala Glu Gly Ala Phe Lys Gly Leu
305 310 315 320
Arg Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser Gly Thr
325 330 335
Ile Glu Asp Thr Ser Gly Ala Phe Ser Gly Leu Asp Ser Leu Ser Lys
340 345 350
Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg Ala Phe
355 360 365
Ser Gly Leu Glu Gly Leu Glu His Leu Asn Leu Gly Gly Asn Ala Ile
370 375 380
Arg Ser Val Gln Phe Asp Ala Phe Val Lys Met Lys Asn Leu Lys Glu
385 390 395 400
Leu His Ile Ser Ser Asp Ser Phe Leu Cys Asp Cys Gln Leu Lys Trp
405 410 415
Leu Pro Pro Trp Leu Ile Gly Arg Met Leu Gln Ala Phe Val Thr Ala
420 425 430
Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe Ser Val
435 440 445
Pro Pro Glu Ser Phe Val Cys Asp Asp Phe Leu Lys Pro Gln Ile Ile
450 455 460
Thr Gln Pro Glu Thr Thr Met Ala Met Val Gly Lys Asp Ile Arg Phe
465 470 475 480
Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe Ala Trp
485 490 495
Lys Lys Asp Asn Glu Val Leu Thr Asn Ala Asp Met Glu Asn Phe Val
500 505 510
His Val His Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr Ile Leu
515 520 525
His Leu Arg Gln Val Thr Phe Gly His Glu Gly Arg Tyr Gln Cys Val
530 535 540
Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg Leu Thr
545 550 555 560
Val Asn Val Leu Pro Ser Phe Thr Lys Thr Pro His Asp Ile Thr Ile
565 570 575
Arg Thr Thr Thr Val Ala Arg Leu Glu Cys Ala Ala Thr Gly His Pro
580 585 590
Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe Pro Ala
595 600 605
Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val Phe Phe
610 615 620
Ile Thr Asp Val Lys Ile Asp Asp Ala Gly Val Tyr Ser Cys Thr Ala
625 630 635 640
Gln Asn Ser Ala Gly Ser Ile Ser Ala Asn Ala Thr Leu Thr Val Leu
645 650 655
Glu Thr Pro Ser Leu Val Val Pro Leu Glu Asp Arg Val Val Ser Val
660 665 670
Gly Glu Thr Val Ala Leu Gln Cys Lys Ala Thr Gly Asn Pro Pro Pro
675 680 685
Arg Ile Thr Trp Phe Lys Gly Asp Arg Pro Leu Ser Leu Thr Glu Arg
690 695 700
His His Leu Thr Pro Asp Asn Gln Leu Leu Val Val Gln Asn Val Val
705 710 715 720
Ala Glu Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Thr Leu Gly
725 730 735
Thr Glu Arg Ala His Ser Gln Leu Ser Val Leu Pro Ala Ala Gly Cys
740 745 750
Arg Lys Asp Gly Thr Thr Val Gly Ile Phe Glu Pro Arg Gly Pro Thr
755 760 765
Ile Lys Pro Cys Pro Pro Cys Lys Cys Pro Ala Pro Asn Leu Leu Gly
770 775 780
Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met
785 790 795 800
Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His
805 810 815
Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val
820 825 830
His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
835 840 845
Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly
850 855 860
Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile
865 870 875 880
Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val
885 890 895
Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser
900 905 910
Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu
915 920 925
Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro
930 935 940
Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val
945 950 955 960
Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
965 970 975
His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
980 985 990
Pro Gly Lys
995
<210> 15
<211> 1007
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 15
Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala Gly Asp
1 5 10 15
Ser Leu Asp Cys Gly Gly Arg Gly Leu Ala Ala Leu Pro Gly Asp Leu
20 25 30
Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Lys Leu Ser Glu
35 40 45
Ile Asp Pro Ala Gly Phe Glu Asp Leu Pro Asn Leu Gln Glu Val Tyr
50 55 60
Leu Asn Asn Asn Glu Leu Thr Ala Val Pro Ser Leu Gly Ala Ala Ser
65 70 75 80
Ser His Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Arg Ser Val
85 90 95
Glu Gly Ser Gln Leu Lys Ala Tyr Leu Ser Leu Glu Val Leu Asp Leu
100 105 110
Ser Leu Asn Asn Ile Thr Glu Val Arg Asn Thr Cys Phe Pro His Gly
115 120 125
Pro Pro Ile Lys Glu Leu Asn Leu Ala Gly Asn Arg Ile Gly Thr Leu
130 135 140
Glu Leu Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr Leu Arg
145 150 155 160
Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Arg Ala Phe Lys Leu
165 170 175
Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg Leu Ile
180 185 190
Glu Gly Leu Thr Phe Gln Gly Leu Asn Ser Leu Glu Val Leu Lys Leu
195 200 205
Gln Arg Asn Asn Ile Ser Lys Leu Thr Asp Gly Ala Phe Trp Gly Leu
210 215 220
Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val Glu Val
225 230 235 240
Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu His Leu
245 250 255
Ser Asn Asn Ser Ile Ala Arg Ile His Arg Lys Gly Trp Ser Phe Cys
260 265 270
Gln Lys Leu His Glu Leu Val Leu Ser Phe Asn Asn Leu Thr Arg Leu
275 280 285
Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Val Leu Arg Leu
290 295 300
Ser His Asn Ser Ile Ser His Ile Ala Glu Gly Ala Phe Lys Gly Leu
305 310 315 320
Arg Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser Gly Thr
325 330 335
Ile Glu Asp Thr Ser Gly Ala Phe Ser Gly Leu Asp Ser Leu Ser Lys
340 345 350
Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg Ala Phe
355 360 365
Ser Gly Leu Glu Gly Leu Glu His Leu Asn Leu Gly Gly Asn Ala Ile
370 375 380
Arg Ser Val Gln Phe Asp Ala Phe Val Lys Met Lys Asn Leu Lys Glu
385 390 395 400
Leu His Ile Ser Ser Asp Ser Phe Leu Cys Asp Cys Gln Leu Lys Trp
405 410 415
Leu Pro Pro Trp Leu Ile Gly Arg Met Leu Gln Ala Phe Val Thr Ala
420 425 430
Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe Ser Val
435 440 445
Pro Pro Glu Ser Phe Val Cys Asp Asp Phe Leu Lys Pro Gln Ile Ile
450 455 460
Thr Gln Pro Glu Thr Thr Met Ala Met Val Gly Lys Asp Ile Arg Phe
465 470 475 480
Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe Ala Trp
485 490 495
Lys Lys Asp Asn Glu Val Leu Thr Asn Ala Asp Met Glu Asn Phe Val
500 505 510
His Val His Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr Ile Leu
515 520 525
His Leu Arg Gln Val Thr Phe Gly His Glu Gly Arg Tyr Gln Cys Val
530 535 540
Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg Leu Thr
545 550 555 560
Val Asn Val Leu Pro Ser Phe Thr Lys Thr Pro His Asp Ile Thr Ile
565 570 575
Arg Thr Thr Thr Val Ala Arg Leu Glu Cys Ala Ala Thr Gly His Pro
580 585 590
Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe Pro Ala
595 600 605
Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val Phe Phe
610 615 620
Ile Thr Asp Val Lys Ile Asp Asp Ala Gly Val Tyr Ser Cys Thr Ala
625 630 635 640
Gln Asn Ser Ala Gly Ser Ile Ser Ala Asn Ala Thr Leu Thr Val Leu
645 650 655
Glu Thr Pro Ser Leu Val Val Pro Leu Glu Asp Arg Val Val Ser Val
660 665 670
Gly Glu Thr Val Ala Leu Gln Cys Lys Ala Thr Gly Asn Pro Pro Pro
675 680 685
Arg Ile Thr Trp Phe Lys Gly Asp Arg Pro Leu Ser Leu Thr Glu Arg
690 695 700
His His Leu Thr Pro Asp Asn Gln Leu Leu Val Val Gln Asn Val Val
705 710 715 720
Ala Glu Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Thr Leu Gly
725 730 735
Thr Glu Arg Ala His Ser Gln Leu Ser Val Leu Pro Ala Ala Gly Cys
740 745 750
Arg Lys Asp Gly Thr Thr Val Gly Ile Phe Arg Asn Thr Gly Arg Gly
755 760 765
Gly Glu Glu Lys Lys Lys Glu Lys Glu Lys Glu Glu Gln Glu Glu Arg
770 775 780
Glu Thr Lys Thr Pro Glu Cys Pro Ser His Thr Gln Pro Leu Gly Val
785 790 795 800
Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr
805 810 815
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu
820 825 830
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys
835 840 845
Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser
850 855 860
Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys
865 870 875 880
Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile
885 890 895
Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro
900 905 910
Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu
915 920 925
Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn
930 935 940
Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser
945 950 955 960
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg
965 970 975
Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu
980 985 990
His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
995 1000 1005
<210> 16
<211> 994
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 16
Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala Gly Asp
1 5 10 15
Ser Leu Asp Cys Gly Gly Arg Gly Leu Ala Ala Leu Pro Gly Asp Leu
20 25 30
Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Lys Leu Ser Glu
35 40 45
Ile Asp Pro Ala Gly Phe Glu Asp Leu Pro Asn Leu Gln Glu Val Tyr
50 55 60
Leu Asn Asn Asn Glu Leu Thr Ala Val Pro Ser Leu Gly Ala Ala Ser
65 70 75 80
Ser His Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Arg Ser Val
85 90 95
Glu Gly Ser Gln Leu Lys Ala Tyr Leu Ser Leu Glu Val Leu Asp Leu
100 105 110
Ser Leu Asn Asn Ile Thr Glu Val Arg Asn Thr Cys Phe Pro His Gly
115 120 125
Pro Pro Ile Lys Glu Leu Asn Leu Ala Gly Asn Arg Ile Gly Thr Leu
130 135 140
Glu Leu Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr Leu Arg
145 150 155 160
Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Arg Ala Phe Lys Leu
165 170 175
Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg Leu Ile
180 185 190
Glu Gly Leu Thr Phe Gln Gly Leu Asn Ser Leu Glu Val Leu Lys Leu
195 200 205
Gln Arg Asn Asn Ile Ser Lys Leu Thr Asp Gly Ala Phe Trp Gly Leu
210 215 220
Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val Glu Val
225 230 235 240
Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu His Leu
245 250 255
Ser Asn Asn Ser Ile Ala Arg Ile His Arg Lys Gly Trp Ser Phe Cys
260 265 270
Gln Lys Leu His Glu Leu Val Leu Ser Phe Asn Asn Leu Thr Arg Leu
275 280 285
Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Val Leu Arg Leu
290 295 300
Ser His Asn Ser Ile Ser His Ile Ala Glu Gly Ala Phe Lys Gly Leu
305 310 315 320
Arg Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser Gly Thr
325 330 335
Ile Glu Asp Thr Ser Gly Ala Phe Ser Gly Leu Asp Ser Leu Ser Lys
340 345 350
Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg Ala Phe
355 360 365
Ser Gly Leu Glu Gly Leu Glu His Leu Asn Leu Gly Gly Asn Ala Ile
370 375 380
Arg Ser Val Gln Phe Asp Ala Phe Val Lys Met Lys Asn Leu Lys Glu
385 390 395 400
Leu His Ile Ser Ser Asp Ser Phe Leu Cys Asp Cys Gln Leu Lys Trp
405 410 415
Leu Pro Pro Trp Leu Ile Gly Arg Met Leu Gln Ala Phe Val Thr Ala
420 425 430
Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe Ser Val
435 440 445
Pro Pro Glu Ser Phe Val Cys Asp Asp Phe Leu Lys Pro Gln Ile Ile
450 455 460
Thr Gln Pro Glu Thr Thr Met Ala Met Val Gly Lys Asp Ile Arg Phe
465 470 475 480
Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe Ala Trp
485 490 495
Lys Lys Asp Asn Glu Val Leu Thr Asn Ala Asp Met Glu Asn Phe Val
500 505 510
His Val His Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr Ile Leu
515 520 525
His Leu Arg Gln Val Thr Phe Gly His Glu Gly Arg Tyr Gln Cys Val
530 535 540
Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg Leu Thr
545 550 555 560
Val Asn Val Leu Pro Ser Phe Thr Lys Thr Pro His Asp Ile Thr Ile
565 570 575
Arg Thr Thr Thr Val Ala Arg Leu Glu Cys Ala Ala Thr Gly His Pro
580 585 590
Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe Pro Ala
595 600 605
Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val Phe Phe
610 615 620
Ile Thr Asp Val Lys Ile Asp Asp Ala Gly Val Tyr Ser Cys Thr Ala
625 630 635 640
Gln Asn Ser Ala Gly Ser Ile Ser Ala Asn Ala Thr Leu Thr Val Leu
645 650 655
Glu Thr Pro Ser Leu Val Val Pro Leu Glu Asp Arg Val Val Ser Val
660 665 670
Gly Glu Thr Val Ala Leu Gln Cys Lys Ala Thr Gly Asn Pro Pro Pro
675 680 685
Arg Ile Thr Trp Phe Lys Gly Asp Arg Pro Leu Ser Leu Thr Glu Arg
690 695 700
His His Leu Thr Pro Asp Asn Gln Leu Leu Val Val Gln Asn Val Val
705 710 715 720
Ala Glu Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Thr Leu Gly
725 730 735
Thr Glu Arg Ala His Ser Gln Leu Ser Val Leu Pro Ala Ala Gly Cys
740 745 750
Arg Lys Asp Gly Thr Thr Val Gly Ile Phe Glu Pro Lys Ser Ser Asp
755 760 765
Lys Thr His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly
770 775 780
Ser Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile
785 790 795 800
Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu
805 810 815
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
820 825 830
Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg
835 840 845
Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
850 855 860
Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu
865 870 875 880
Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr
885 890 895
Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu
900 905 910
Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp
915 920 925
Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val
930 935 940
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp
945 950 955 960
Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His
965 970 975
Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro
980 985 990
Gly Lys
<210> 17
<211> 2982
<212> DNA
<213> 人工序列(Artificial Sequence)
<223> 融合蛋白(fusion protein)
<400> 17
gctgggcctc gggctccttg tgctgccgcc tgcacatgtg caggcgattc cctggactgc 60
ggcggcagag gcctggccgc cctgcctggc gatctgccat cctggacccg gagcctgaac 120
ctgagctaca acaagctgag cgagatcgat cccgccggct ttgaggacct gcctaacctg 180
caggaggtgt atctgaacaa taacgagctg accgcggtac catccctggg cgctgcttca 240
tcacatgtcg tctctctctt tctgcagcac aacaagattc gcagcgtgga ggggagccag 300
ctgaaggcct acctttcctt agaagtgtta gatctgagtt tgaacaacat cacggaagtg 360
cggaacacct gctttccaca cggaccgcct ataaaggagc tcaacctggc aggcaatcgg 420
attggcaccc tggagttggg agcatttgat ggtctgtcac ggtcgctgct aactcttcgc 480
ctgagcaaaa acaggatcac ccagcttcct gtaagagcat tcaagctacc caggctgaca 540
caactggacc tcaatcggaa caggattcgg ctgatagagg gcctcacctt ccaggggctc 600
aacagcttgg aggtgctgaa gcttcagcga aacaacatca gcaaactgac agatggggcc 660
ttctggggac tgtccaagat gcatgtgctg cacctggagt acaacagcct ggtagaagtg 720
aacagcggct cgctctacgg cctcacggcc ctgcatcagc tccacctcag caacaattcc 780
atcgctcgca ttcaccgcaa gggctggagc ttctgccaga agctgcatga gttggtcctg 840
tccttcaaca acctgacacg gctggacgag gagagcctgg ccgagctgag cagcctgagt 900
gtcctgcgtc tcagccacaa ttccatcagc cacattgcgg agggtgcctt caagggactc 960
aggagcctgc gagtcttgga tctggaccat aacgagattt cgggcacaat agaggacacg 1020
agcggcgcct tctcagggct cgacagcctc agcaagctga ctctgtttgg aaacaagatc 1080
aagtctgtgg ctaagagagc attctcgggg ctggaaggcc tggagcacct gaaccttgga 1140
gggaatgcga tcagatctgt ccagtttgat gcctttgtga agatgaagaa tcttaaagag 1200
ctccatatca gcagcgacag cttcctgtgt gactgccagc tgaagtggct gcccccgtgg 1260
ctaattggca ggatgctgca ggcctttgtg acagccacct gtgcccaccc agaatcactg 1320
aagggtcaga gcattttctc tgtgccacca gagagtttcg tgtgcgatga cttcctgaag 1380
ccacagatca tcacccagcc agaaaccacc atggctatgg tgggcaagga catccggttt 1440
acatgctcag cagccagcag cagcagctcc cccatgacct ttgcctggaa gaaagacaat 1500
gaagtcctga ccaatgcaga catggagaac tttgtccacg tccacgcgca ggacggggaa 1560
gtgatggagt acaccaccat cctgcacctc cgtcaggtca ctttcgggca cgagggccgc 1620
taccaatgtg tcatcaccaa ccactttggc tccacctatt cacataaggc caggctcacc 1680
gtgaatgtgt tgccatcatt caccaaaacg ccccacgaca taaccatccg gaccaccacc 1740
gtggcccgcc tcgaatgtgc tgccacaggt cacccaaacc ctcagattgc ctggcagaag 1800
gatggaggca cggatttccc cgctgcccgt gagcgacgca tgcatgtcat gccggatgac 1860
gacgtgtttt tcatcactga tgtgaaaata gatgacgcag gggtttacag ctgtactgct 1920
cagaactcag ccggttctat ttcagctaat gccaccctga ctgtcctaga gaccccatcc 1980
ttggtggtcc ccttggaaga ccgtgtggta tctgtgggag aaacagtggc cctccaatgc 2040
aaagccacgg ggaaccctcc gccccgcatc acctggttca agggggaccg cccgctgagc 2100
ctcactgagc ggcaccacct gacccctgac aaccagctcc tggtggttca gaacgtggtg 2160
gcagaggatg cgggccgata tacctgtgag atgtccaaca ccctgggcac ggagcgagct 2220
cacagccagc tgagcgtcct gcccgcagca ggctgcagga aggatgggac cacggtaggc 2280
atcttcgagc caaagtcctc tgataagaca cacacctctc caccatgccc agcaccagag 2340
ctgctgggag gaccaagcgt gttcctgttt cctccaaagc ccaaggacac actgatgatc 2400
tccaggacac cagaggtgac ctgcgtggtg gtggacgtga gccacgagga ccccgaggtg 2460
aagttcaact ggtacgtgga tggcgtggag gtgcacaatg ccaagaccaa gcccagagag 2520
gagcagtaca actctaccta tagggtggtg agcgtgctga cagtgctgca ccaggactgg 2580
ctgaacggca aggagtataa gtgcaaggtg agcaataagg ccctgcctgc cccaatcgag 2640
aagacaatct ccaaggccaa gggccagcca agagagcccc aggtgtacac cctgccccct 2700
agcagggatg agctgacaaa gaaccaggtg tccctgacct gtctggtgaa gggcttttat 2760
ccctccgaca tcgccgtgga gtgggagtct aatggccagc ctgagaataa ctacaagaca 2820
accccacccg tgctggattc tgacggcagc ttctttctgt attctaagct gaccgtggac 2880
aagagcaggt ggcagcaggg caacgtgttc agctgctccg tgatgcacga agcactgcac 2940
aatcactaca cccagaaatc actgtcactg agccctggca aa 2982
<210> 18
<211> 2985
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 18
gctgggcctc gggctccttg tgctgccgcc tgcacatgtg caggcgattc cctggactgc 60
ggcggcagag gcctggccgc cctgcctggc gatctgccat cctggacccg gagcctgaac 120
ctgagctaca acaagctgag cgagatcgat cccgccggct ttgaggacct gcctaacctg 180
caggaggtgt atctgaacaa taacgagctg accgcggtac catccctggg cgctgcttca 240
tcacatgtcg tctctctctt tctgcagcac aacaagattc gcagcgtgga ggggagccag 300
ctgaaggcct acctttcctt agaagtgtta gatctgagtt tgaacaacat cacggaagtg 360
cggaacacct gctttccaca cggaccgcct ataaaggagc tcaacctggc aggcaatcgg 420
attggcaccc tggagttggg agcatttgat ggtctgtcac ggtcgctgct aactcttcgc 480
ctgagcaaaa acaggatcac ccagcttcct gtaagagcat tcaagctacc caggctgaca 540
caactggacc tcaatcggaa caggattcgg ctgatagagg gcctcacctt ccaggggctc 600
aacagcttgg aggtgctgaa gcttcagcga aacaacatca gcaaactgac agatggggcc 660
ttctggggac tgtccaagat gcatgtgctg cacctggagt acaacagcct ggtagaagtg 720
aacagcggct cgctctacgg cctcacggcc ctgcatcagc tccacctcag caacaattcc 780
atcgctcgca ttcaccgcaa gggctggagc ttctgccaga agctgcatga gttggtcctg 840
tccttcaaca acctgacacg gctggacgag gagagcctgg ccgagctgag cagcctgagt 900
gtcctgcgtc tcagccacaa ttccatcagc cacattgcgg agggtgcctt caagggactc 960
aggagcctgc gagtcttgga tctggaccat aacgagattt cgggcacaat agaggacacg 1020
agcggcgcct tctcagggct cgacagcctc agcaagctga ctctgtttgg aaacaagatc 1080
aagtctgtgg ctaagagagc attctcgggg ctggaaggcc tggagcacct gaaccttgga 1140
gggaatgcga tcagatctgt ccagtttgat gcctttgtga agatgaagaa tcttaaagag 1200
ctccatatca gcagcgacag cttcctgtgt gactgccagc tgaagtggct gcccccgtgg 1260
ctaattggca ggatgctgca ggcctttgtg acagccacct gtgcccaccc agaatcactg 1320
aagggtcaga gcattttctc tgtgccacca gagagtttcg tgtgcgatga cttcctgaag 1380
ccacagatca tcacccagcc agaaaccacc atggctatgg tgggcaagga catccggttt 1440
acatgctcag cagccagcag cagcagctcc cccatgacct ttgcctggaa gaaagacaat 1500
gaagtcctga ccaatgcaga catggagaac tttgtccacg tccacgcgca ggacggggaa 1560
gtgatggagt acaccaccat cctgcacctc cgtcaggtca ctttcgggca cgagggccgc 1620
taccaatgtg tcatcaccaa ccactttggc tccacctatt cacataaggc caggctcacc 1680
gtgaatgtgt tgccatcatt caccaaaacg ccccacgaca taaccatccg gaccaccacc 1740
gtggcccgcc tcgaatgtgc tgccacaggt cacccaaacc ctcagattgc ctggcagaag 1800
gatggaggca cggatttccc cgctgcccgt gagcgacgca tgcatgtcat gccggatgac 1860
gacgtgtttt tcatcactga tgtgaaaata gatgacgcag gggtttacag ctgtactgct 1920
cagaactcag ccggttctat ttcagctaat gccaccctga ctgtcctaga gaccccatcc 1980
ttggtggtcc ccttggaaga ccgtgtggta tctgtgggag aaacagtggc cctccaatgc 2040
aaagccacgg ggaaccctcc gccccgcatc acctggttca agggggaccg cccgctgagc 2100
ctcactgagc ggcaccacct gacccctgac aaccagctcc tggtggttca gaacgtggtg 2160
gcagaggatg cgggccgata tacctgtgag atgtccaaca ccctgggcac ggagcgagct 2220
cacagccagc tgagcgtcct gcccgcagca ggctgcagga aggatgggac cacggtaggc 2280
atcttcgagc ctcggggccc taccatcaag ccctgccccc cttgcaagtg ccctgcccct 2340
aatctgctgg gcggaccctc cgtgttcctg tttcctccaa agcccaagga cacactgatg 2400
atctccagga caccagaggt gacctgcgtg gtggtggacg tgagccacga ggaccccgag 2460
gtgaagttca actggtacgt ggatggcgtg gaggtgcaca atgccaagac caagcccaga 2520
gaggagcagt acaactctac ctatagggtg gtgagcgtgc tgacagtgct gcaccaggac 2580
tggctgaacg gcaaggagta taagtgcaag gtgagcaata aggccctgcc tgccccaatc 2640
gagaagacaa tctccaaggc caagggccag ccaagagagc cccaggtgta caccctgccc 2700
cctagcaggg atgagctgac aaagaaccag gtgtccctga cctgtctggt gaagggcttt 2760
tatccctccg acatcgccgt ggagtgggag tctaatggcc agcctgagaa taactacaag 2820
acaaccccac ccgtgctgga ttctgacggc agcttctttc tgtattctaa gctgaccgtg 2880
gacaagagca ggtggcagca gggcaacgtg ttcagctgct ccgtgatgca cgaagcactg 2940
cacaatcact acacccagaa atcactgtca ctgagccctg gcaaa 2985
<210> 19
<211> 3021
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 19
gctgggcctc gggctccttg tgctgccgcc tgcacatgtg caggcgattc cctggactgc 60
ggcggcagag gcctggccgc cctgcctggc gatctgccat cctggacccg gagcctgaac 120
ctgagctaca acaagctgag cgagatcgat cccgccggct ttgaggacct gcctaacctg 180
caggaggtgt atctgaacaa taacgagctg accgcggtac catccctggg cgctgcttca 240
tcacatgtcg tctctctctt tctgcagcac aacaagattc gcagcgtgga ggggagccag 300
ctgaaggcct acctttcctt agaagtgtta gatctgagtt tgaacaacat cacggaagtg 360
cggaacacct gctttccaca cggaccgcct ataaaggagc tcaacctggc aggcaatcgg 420
attggcaccc tggagttggg agcatttgat ggtctgtcac ggtcgctgct aactcttcgc 480
ctgagcaaaa acaggatcac ccagcttcct gtaagagcat tcaagctacc caggctgaca 540
caactggacc tcaatcggaa caggattcgg ctgatagagg gcctcacctt ccaggggctc 600
aacagcttgg aggtgctgaa gcttcagcga aacaacatca gcaaactgac agatggggcc 660
ttctggggac tgtccaagat gcatgtgctg cacctggagt acaacagcct ggtagaagtg 720
aacagcggct cgctctacgg cctcacggcc ctgcatcagc tccacctcag caacaattcc 780
atcgctcgca ttcaccgcaa gggctggagc ttctgccaga agctgcatga gttggtcctg 840
tccttcaaca acctgacacg gctggacgag gagagcctgg ccgagctgag cagcctgagt 900
gtcctgcgtc tcagccacaa ttccatcagc cacattgcgg agggtgcctt caagggactc 960
aggagcctgc gagtcttgga tctggaccat aacgagattt cgggcacaat agaggacacg 1020
agcggcgcct tctcagggct cgacagcctc agcaagctga ctctgtttgg aaacaagatc 1080
aagtctgtgg ctaagagagc attctcgggg ctggaaggcc tggagcacct gaaccttgga 1140
gggaatgcga tcagatctgt ccagtttgat gcctttgtga agatgaagaa tcttaaagag 1200
ctccatatca gcagcgacag cttcctgtgt gactgccagc tgaagtggct gcccccgtgg 1260
ctaattggca ggatgctgca ggcctttgtg acagccacct gtgcccaccc agaatcactg 1320
aagggtcaga gcattttctc tgtgccacca gagagtttcg tgtgcgatga cttcctgaag 1380
ccacagatca tcacccagcc agaaaccacc atggctatgg tgggcaagga catccggttt 1440
acatgctcag cagccagcag cagcagctcc cccatgacct ttgcctggaa gaaagacaat 1500
gaagtcctga ccaatgcaga catggagaac tttgtccacg tccacgcgca ggacggggaa 1560
gtgatggagt acaccaccat cctgcacctc cgtcaggtca ctttcgggca cgagggccgc 1620
taccaatgtg tcatcaccaa ccactttggc tccacctatt cacataaggc caggctcacc 1680
gtgaatgtgt tgccatcatt caccaaaacg ccccacgaca taaccatccg gaccaccacc 1740
gtggcccgcc tcgaatgtgc tgccacaggt cacccaaacc ctcagattgc ctggcagaag 1800
gatggaggca cggatttccc cgctgcccgt gagcgacgca tgcatgtcat gccggatgac 1860
gacgtgtttt tcatcactga tgtgaaaata gatgacgcag gggtttacag ctgtactgct 1920
cagaactcag ccggttctat ttcagctaat gccaccctga ctgtcctaga gaccccatcc 1980
ttggtggtcc ccttggaaga ccgtgtggta tctgtgggag aaacagtggc cctccaatgc 2040
aaagccacgg ggaaccctcc gccccgcatc acctggttca agggggaccg cccgctgagc 2100
ctcactgagc ggcaccacct gacccctgac aaccagctcc tggtggttca gaacgtggtg 2160
gcagaggatg cgggccgata tacctgtgag atgtccaaca ccctgggcac ggagcgagct 2220
cacagccagc tgagcgtcct gcccgcagca ggctgcagga aggatgggac cacggtaggc 2280
atcttccgca acaccggccg cggcggcgag gagaagaaga aggagaagga gaaggaggag 2340
caggaggagc gcgagaccaa gacccccgag tgccccagcc acacccagcc cctgggcgtg 2400
ttcctgtttc ctccaaagcc caaggacaca ctgatgatct ccaggacacc agaggtgacc 2460
tgcgtggtgg tggacgtgag ccacgaggac cccgaggtga agttcaactg gtacgtggat 2520
ggcgtggagg tgcacaatgc caagaccaag cccagagagg agcagtacaa ctctacctat 2580
agggtggtga gcgtgctgac agtgctgcac caggactggc tgaacggcaa ggagtataag 2640
tgcaaggtga gcaataaggc cctgcctgcc ccaatcgaga agacaatctc caaggccaag 2700
ggccagccaa gagagcccca ggtgtacacc ctgcccccta gcagggatga gctgacaaag 2760
aaccaggtgt ccctgacctg tctggtgaag ggcttttatc cctccgacat cgccgtggag 2820
tgggagtcta atggccagcc tgagaataac tacaagacaa ccccacccgt gctggattct 2880
gacggcagct tctttctgta ttctaagctg accgtggaca agagcaggtg gcagcagggc 2940
aacgtgttca gctgctccgt gatgcacgaa gcactgcaca atcactacac ccagaaatca 3000
ctgtcactga gccctggcaa a 3021
<210> 20
<211> 2982
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 20
gctgggcctc gggctccttg tgctgccgcc tgcacatgtg caggcgattc cctggactgc 60
ggcggcagag gcctggccgc cctgcctggc gatctgccat cctggacccg gagcctgaac 120
ctgagctaca acaagctgag cgagatcgat cccgccggct ttgaggacct gcctaacctg 180
caggaggtgt atctgaacaa taacgagctg accgcggtac catccctggg cgctgcttca 240
tcacatgtcg tctctctctt tctgcagcac aacaagattc gcagcgtgga ggggagccag 300
ctgaaggcct acctttcctt agaagtgtta gatctgagtt tgaacaacat cacggaagtg 360
cggaacacct gctttccaca cggaccgcct ataaaggagc tcaacctggc aggcaatcgg 420
attggcaccc tggagttggg agcatttgat ggtctgtcac ggtcgctgct aactcttcgc 480
ctgagcaaaa acaggatcac ccagcttcct gtaagagcat tcaagctacc caggctgaca 540
caactggacc tcaatcggaa caggattcgg ctgatagagg gcctcacctt ccaggggctc 600
aacagcttgg aggtgctgaa gcttcagcga aacaacatca gcaaactgac agatggggcc 660
ttctggggac tgtccaagat gcatgtgctg cacctggagt acaacagcct ggtagaagtg 720
aacagcggct cgctctacgg cctcacggcc ctgcatcagc tccacctcag caacaattcc 780
atcgctcgca ttcaccgcaa gggctggagc ttctgccaga agctgcatga gttggtcctg 840
tccttcaaca acctgacacg gctggacgag gagagcctgg ccgagctgag cagcctgagt 900
gtcctgcgtc tcagccacaa ttccatcagc cacattgcgg agggtgcctt caagggactc 960
aggagcctgc gagtcttgga tctggaccat aacgagattt cgggcacaat agaggacacg 1020
agcggcgcct tctcagggct cgacagcctc agcaagctga ctctgtttgg aaacaagatc 1080
aagtctgtgg ctaagagagc attctcgggg ctggaaggcc tggagcacct gaaccttgga 1140
gggaatgcga tcagatctgt ccagtttgat gcctttgtga agatgaagaa tcttaaagag 1200
ctccatatca gcagcgacag cttcctgtgt gactgccagc tgaagtggct gcccccgtgg 1260
ctaattggca ggatgctgca ggcctttgtg acagccacct gtgcccaccc agaatcactg 1320
aagggtcaga gcattttctc tgtgccacca gagagtttcg tgtgcgatga cttcctgaag 1380
ccacagatca tcacccagcc agaaaccacc atggctatgg tgggcaagga catccggttt 1440
acatgctcag cagccagcag cagcagctcc cccatgacct ttgcctggaa gaaagacaat 1500
gaagtcctga ccaatgcaga catggagaac tttgtccacg tccacgcgca ggacggggaa 1560
gtgatggagt acaccaccat cctgcacctc cgtcaggtca ctttcgggca cgagggccgc 1620
taccaatgtg tcatcaccaa ccactttggc tccacctatt cacataaggc caggctcacc 1680
gtgaatgtgt tgccatcatt caccaaaacg ccccacgaca taaccatccg gaccaccacc 1740
gtggcccgcc tcgaatgtgc tgccacaggt cacccaaacc ctcagattgc ctggcagaag 1800
gatggaggca cggatttccc cgctgcccgt gagcgacgca tgcatgtcat gccggatgac 1860
gacgtgtttt tcatcactga tgtgaaaata gatgacgcag gggtttacag ctgtactgct 1920
cagaactcag ccggttctat ttcagctaat gccaccctga ctgtcctaga gaccccatcc 1980
ttggtggtcc ccttggaaga ccgtgtggta tctgtgggag aaacagtggc cctccaatgc 2040
aaagccacgg ggaaccctcc gccccgcatc acctggttca agggggaccg cccgctgagc 2100
ctcactgagc ggcaccacct gacccctgac aaccagctcc tggtggttca gaacgtggtg 2160
gcagaggatg cgggccgata tacctgtgag atgtccaaca ccctgggcac ggagcgagct 2220
cacagccagc tgagcgtcct gcccgcagca ggctgcagga aggatgggac cacggtaggc 2280
atcttcgaac cgaaatcttc tgacaaaacc cacacctctc cgccgtctcc ggctccggaa 2340
ctgctgggtg gttcttctgt tttcctgttt cctccaaagc ccaaggacac actgatgatc 2400
tccaggacac cagaggtgac ctgcgtggtg gtggacgtga gccacgagga ccccgaggtg 2460
aagttcaact ggtacgtgga tggcgtggag gtgcacaatg ccaagaccaa gcccagagag 2520
gagcagtaca actctaccta tagggtggtg agcgtgctga cagtgctgca ccaggactgg 2580
ctgaacggca aggagtataa gtgcaaggtg agcaataagg ccctgcctgc cccaatcgag 2640
aagacaatct ccaaggccaa gggccagcca agagagcccc aggtgtacac cctgccccct 2700
agcagggatg agctgacaaa gaaccaggtg tccctgacct gtctggtgaa gggcttttat 2760
ccctccgaca tcgccgtgga gtgggagtct aatggccagc ctgagaataa ctacaagaca 2820
accccacccg tgctggattc tgacggcagc ttctttctgt attctaagct gaccgtggac 2880
aagagcaggt ggcagcaggg caacgtgttc agctgctccg tgatgcacga agcactgcac 2940
aatcactaca cccagaaatc actgtcactg agccctggca aa 2982
<210> 21
<211> 992
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 21
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Glu Pro Lys Ser Ser Asp Lys Thr
755 760 765
His Thr Ser Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser
770 775 780
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
785 790 795 800
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
805 810 815
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
820 825 830
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
835 840 845
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
850 855 860
Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
865 870 875 880
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
885 890 895
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys
900 905 910
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
915 920 925
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
930 935 940
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
945 950 955 960
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
965 970 975
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
980 985 990
<210> 22
<211> 993
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 22
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Glu Pro Arg Gly Pro Thr Ile Lys
755 760 765
Pro Cys Pro Pro Cys Lys Cys Pro Ala Pro Asn Leu Leu Gly Gly Pro
770 775 780
Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser
785 790 795 800
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp
805 810 815
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn
820 825 830
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val
835 840 845
Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu
850 855 860
Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys
865 870 875 880
Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr
885 890 895
Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr
900 905 910
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu
915 920 925
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu
930 935 940
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys
945 950 955 960
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu
965 970 975
Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
980 985 990
Lys
<210> 23
<211> 1005
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 23
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Arg Asn Thr Gly Arg Gly Gly Glu
755 760 765
Glu Lys Lys Lys Glu Lys Glu Lys Glu Glu Gln Glu Glu Arg Glu Thr
770 775 780
Lys Thr Pro Glu Cys Pro Ser His Thr Gln Pro Leu Gly Val Phe Leu
785 790 795 800
Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu
805 810 815
Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys
820 825 830
Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys
835 840 845
Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu
850 855 860
Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys
865 870 875 880
Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys
885 890 895
Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser
900 905 910
Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys
915 920 925
Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln
930 935 940
Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly
945 950 955 960
Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln
965 970 975
Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn
980 985 990
His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
995 1000 1005
<210> 24
<211> 992
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 24
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Glu Pro Lys Ser Ser Asp Lys Thr
755 760 765
His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly Ser Ser
770 775 780
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg
785 790 795 800
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro
805 810 815
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala
820 825 830
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val
835 840 845
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr
850 855 860
Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr
865 870 875 880
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu
885 890 895
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys
900 905 910
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser
915 920 925
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp
930 935 940
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser
945 950 955 960
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala
965 970 975
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
980 985 990
<210> 25
<211> 2976
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 25
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
gagccaaagt cctctgataa gacacacacc tctccaccat gcccagcacc agagctgctg 2340
ggaggaccaa gcgtgttcct gtttcctcca aagcccaagg acacactgat gatctccagg 2400
acaccagagg tgacctgcgt ggtggtggac gtgagccacg aggaccccga ggtgaagttc 2460
aactggtacg tggatggcgt ggaggtgcac aatgccaaga ccaagcccag agaggagcag 2520
tacaactcta cctatagggt ggtgagcgtg ctgacagtgc tgcaccagga ctggctgaac 2580
ggcaaggagt ataagtgcaa ggtgagcaat aaggccctgc ctgccccaat cgagaagaca 2640
atctccaagg ccaagggcca gccaagagag ccccaggtgt acaccctgcc ccctagcagg 2700
gatgagctga caaagaacca ggtgtccctg acctgtctgg tgaagggctt ttatccctcc 2760
gacatcgccg tggagtggga gtctaatggc cagcctgaga ataactacaa gacaacccca 2820
cccgtgctgg attctgacgg cagcttcttt ctgtattcta agctgaccgt ggacaagagc 2880
aggtggcagc agggcaacgt gttcagctgc tccgtgatgc acgaagcact gcacaatcac 2940
tacacccaga aatcactgtc actgagccct ggcaaa 2976
<210> 26
<211> 2979
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 26
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
gagcctcggg gccctaccat caagccctgc cccccttgca agtgccctgc ccctaatctg 2340
ctgggcggac cctccgtgtt cctgtttcct ccaaagccca aggacacact gatgatctcc 2400
aggacaccag aggtgacctg cgtggtggtg gacgtgagcc acgaggaccc cgaggtgaag 2460
ttcaactggt acgtggatgg cgtggaggtg cacaatgcca agaccaagcc cagagaggag 2520
cagtacaact ctacctatag ggtggtgagc gtgctgacag tgctgcacca ggactggctg 2580
aacggcaagg agtataagtg caaggtgagc aataaggccc tgcctgcccc aatcgagaag 2640
acaatctcca aggccaaggg ccagccaaga gagccccagg tgtacaccct gccccctagc 2700
agggatgagc tgacaaagaa ccaggtgtcc ctgacctgtc tggtgaaggg cttttatccc 2760
tccgacatcg ccgtggagtg ggagtctaat ggccagcctg agaataacta caagacaacc 2820
ccacccgtgc tggattctga cggcagcttc tttctgtatt ctaagctgac cgtggacaag 2880
agcaggtggc agcagggcaa cgtgttcagc tgctccgtga tgcacgaagc actgcacaat 2940
cactacaccc agaaatcact gtcactgagc cctggcaaa 2979
<210> 27
<211> 3015
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 27
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
cgcaacaccg gccgcggcgg cgaggagaag aagaaggaga aggagaagga ggagcaggag 2340
gagcgcgaga ccaagacccc cgagtgcccc agccacaccc agcccctggg cgtgttcctg 2400
tttcctccaa agcccaagga cacactgatg atctccagga caccagaggt gacctgcgtg 2460
gtggtggacg tgagccacga ggaccccgag gtgaagttca actggtacgt ggatggcgtg 2520
gaggtgcaca atgccaagac caagcccaga gaggagcagt acaactctac ctatagggtg 2580
gtgagcgtgc tgacagtgct gcaccaggac tggctgaacg gcaaggagta taagtgcaag 2640
gtgagcaata aggccctgcc tgccccaatc gagaagacaa tctccaaggc caagggccag 2700
ccaagagagc cccaggtgta caccctgccc cctagcaggg atgagctgac aaagaaccag 2760
gtgtccctga cctgtctggt gaagggcttt tatccctccg acatcgccgt ggagtgggag 2820
tctaatggcc agcctgagaa taactacaag acaaccccac ccgtgctgga ttctgacggc 2880
agcttctttc tgtattctaa gctgaccgtg gacaagagca ggtggcagca gggcaacgtg 2940
ttcagctgct ccgtgatgca cgaagcactg cacaatcact acacccagaa atcactgtca 3000
ctgagccctg gcaaa 3015
<210> 28
<211> 2976
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 28
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
gaaccgaaat cttctgacaa aacccacacc tctccgccgt ctccggctcc ggaactgctg 2340
ggtggttctt ctgttttcct gtttcctcca aagcccaagg acacactgat gatctccagg 2400
acaccagagg tgacctgcgt ggtggtggac gtgagccacg aggaccccga ggtgaagttc 2460
aactggtacg tggatggcgt ggaggtgcac aatgccaaga ccaagcccag agaggagcag 2520
tacaactcta cctatagggt ggtgagcgtg ctgacagtgc tgcaccagga ctggctgaac 2580
ggcaaggagt ataagtgcaa ggtgagcaat aaggccctgc ctgccccaat cgagaagaca 2640
atctccaagg ccaagggcca gccaagagag ccccaggtgt acaccctgcc ccctagcagg 2700
gatgagctga caaagaacca ggtgtccctg acctgtctgg tgaagggctt ttatccctcc 2760
gacatcgccg tggagtggga gtctaatggc cagcctgaga ataactacaa gacaacccca 2820
cccgtgctgg attctgacgg cagcttcttt ctgtattcta agctgaccgt ggacaagagc 2880
aggtggcagc agggcaacgt gttcagctgc tccgtgatgc acgaagcact gcacaatcac 2940
tacacccaga aatcactgtc actgagccct ggcaaa 2976
<210> 29
<211> 992
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 29
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Glu Pro Lys Ser Ser Asp Lys Thr
755 760 765
His Thr Ser Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser
770 775 780
Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile Ser Leu
785 790 795 800
Ser Pro Ile Val Thr Cys Val Val Val Asp Val Ser Glu Asp Asp Pro
805 810 815
Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His Thr Ala
820 825 830
Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu Arg Val Val
835 840 845
Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys Glu Phe
850 855 860
Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu Arg Thr
865 870 875 880
Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val Tyr Val Leu
885 890 895
Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr Leu Thr Cys
900 905 910
Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu Trp Thr Asn
915 920 925
Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val Leu Asp
930 935 940
Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu Lys Lys
945 950 955 960
Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val His Glu Gly
965 970 975
Leu His Asn His His Thr Thr Lys Ser Phe Ser Arg Thr Pro Gly Lys
980 985 990
<210> 30
<211> 993
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 30
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Glu Pro Arg Gly Pro Thr Ile Lys
755 760 765
Pro Cys Pro Pro Cys Lys Cys Pro Ala Pro Asn Leu Leu Gly Gly Pro
770 775 780
Ser Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile Ser
785 790 795 800
Leu Ser Pro Ile Val Thr Cys Val Val Val Asp Val Ser Glu Asp Asp
805 810 815
Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His Thr
820 825 830
Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu Arg Val
835 840 845
Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys Glu
850 855 860
Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu Arg
865 870 875 880
Thr Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val Tyr Val
885 890 895
Leu Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr Leu Thr
900 905 910
Cys Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu Trp Thr
915 920 925
Asn Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val Leu
930 935 940
Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu Lys
945 950 955 960
Lys Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val His Glu
965 970 975
Gly Leu His Asn His His Thr Thr Lys Ser Phe Ser Arg Thr Pro Gly
980 985 990
Lys
<210> 31
<211> 1005
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 31
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Arg Asn Thr Gly Arg Gly Gly Glu
755 760 765
Glu Lys Lys Lys Glu Lys Glu Lys Glu Glu Gln Glu Glu Arg Glu Thr
770 775 780
Lys Thr Pro Glu Cys Pro Ser His Thr Gln Pro Leu Gly Val Phe Ile
785 790 795 800
Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile Ser Leu Ser Pro Ile
805 810 815
Val Thr Cys Val Val Val Asp Val Ser Glu Asp Asp Pro Asp Val Gln
820 825 830
Ile Ser Trp Phe Val Asn Asn Val Glu Val His Thr Ala Gln Thr Gln
835 840 845
Thr His Arg Glu Asp Tyr Asn Ser Thr Leu Arg Val Val Ser Ala Leu
850 855 860
Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys Glu Phe Lys Cys Lys
865 870 875 880
Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu Arg Thr Ile Ser Lys
885 890 895
Pro Lys Gly Ser Val Arg Ala Pro Gln Val Tyr Val Leu Pro Pro Pro
900 905 910
Glu Glu Glu Met Thr Lys Lys Gln Val Thr Leu Thr Cys Met Val Thr
915 920 925
Asp Phe Met Pro Glu Asp Ile Tyr Val Glu Trp Thr Asn Asn Gly Lys
930 935 940
Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val Leu Asp Ser Asp Gly
945 950 955 960
Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu Lys Lys Asn Trp Val
965 970 975
Glu Arg Asn Ser Tyr Ser Cys Ser Val Val His Glu Gly Leu His Asn
980 985 990
His His Thr Thr Lys Ser Phe Ser Arg Thr Pro Gly Lys
995 1000 1005
<210> 32
<211> 992
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 32
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Glu Pro Lys Ser Ser Asp Lys Thr
755 760 765
His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly Ser Ser
770 775 780
Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile Ser Leu
785 790 795 800
Ser Pro Ile Val Thr Cys Val Val Val Asp Val Ser Glu Asp Asp Pro
805 810 815
Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His Thr Ala
820 825 830
Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu Arg Val Val
835 840 845
Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys Glu Phe
850 855 860
Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu Arg Thr
865 870 875 880
Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val Tyr Val Leu
885 890 895
Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr Leu Thr Cys
900 905 910
Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu Trp Thr Asn
915 920 925
Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val Leu Asp
930 935 940
Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu Lys Lys
945 950 955 960
Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val His Glu Gly
965 970 975
Leu His Asn His His Thr Thr Lys Ser Phe Ser Arg Thr Pro Gly Lys
980 985 990
<210> 33
<211> 2976
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 33
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
gagccaaagt cctctgataa gacacacacc tctccaccat gcccagcacc agagctgctg 2340
ggaggaccaa gcgtgttcat cttcccaccc aagatcaagg acgtgctgat gatctccctg 2400
tcccccatcg tgacctgcgt ggtggtggac gtgtccgagg acgaccccga cgtgcagatc 2460
agttggttcg tgaacaacgt ggaagtgcac accgcccaga cccagaccca cagagaggac 2520
tacaactcca ccctgcgggt ggtgtccgcc ctgcccatcc agcaccagga ctggatgtcc 2580
ggcaaagaat tcaagtgcaa agtgaacaac aaggacctgc ctgcccccat cgagcggacc 2640
atctccaagc ccaagggctc cgtgcgggct ccccaggtgt acgtgctgcc ccctccagag 2700
gaagagatga ccaagaagca ggtcacactg acctgcatgg tcaccgactt catgcccgag 2760
gacatctacg tggaatggac caacaatggc aagaccgagc tgaactacaa gaacaccgag 2820
cctgtgctgg actccgacgg ctcctacttc atgtactcca agctgcgggt ggaaaagaag 2880
aactgggtcg agcggaactc ctactcctgc tccgtggtgc acgagggcct gcacaaccac 2940
cacaccacca agtccttctc ccggaccccc ggcaaa 2976
<210> 34
<211> 2979
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 34
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
gagcctcggg gccctaccat caagccctgc cccccttgca agtgccctgc ccctaatctg 2340
ctgggcggac cctccgtgtt catcttccca cccaagatca aggacgtgct gatgatctcc 2400
ctgtccccca tcgtgacctg cgtggtggtg gacgtgtccg aggacgaccc cgacgtgcag 2460
atcagttggt tcgtgaacaa cgtggaagtg cacaccgccc agacccagac ccacagagag 2520
gactacaact ccaccctgcg ggtggtgtcc gccctgccca tccagcacca ggactggatg 2580
tccggcaaag aattcaagtg caaagtgaac aacaaggacc tgcctgcccc catcgagcgg 2640
accatctcca agcccaaggg ctccgtgcgg gctccccagg tgtacgtgct gccccctcca 2700
gaggaagaga tgaccaagaa gcaggtcaca ctgacctgca tggtcaccga cttcatgccc 2760
gaggacatct acgtggaatg gaccaacaat ggcaagaccg agctgaacta caagaacacc 2820
gagcctgtgc tggactccga cggctcctac ttcatgtact ccaagctgcg ggtggaaaag 2880
aagaactggg tcgagcggaa ctcctactcc tgctccgtgg tgcacgaggg cctgcacaac 2940
caccacacca ccaagtcctt ctcccggacc cccggcaaa 2979
<210> 35
<211> 3015
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 35
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
cgcaacaccg gccgcggcgg cgaggagaag aagaaggaga aggagaagga ggagcaggag 2340
gagcgcgaga ccaagacccc cgagtgcccc agccacaccc agcccctggg cgtgttcatc 2400
ttcccaccca agatcaagga cgtgctgatg atctccctgt cccccatcgt gacctgcgtg 2460
gtggtggacg tgtccgagga cgaccccgac gtgcagatca gttggttcgt gaacaacgtg 2520
gaagtgcaca ccgcccagac ccagacccac agagaggact acaactccac cctgcgggtg 2580
gtgtccgccc tgcccatcca gcaccaggac tggatgtccg gcaaagaatt caagtgcaaa 2640
gtgaacaaca aggacctgcc tgcccccatc gagcggacca tctccaagcc caagggctcc 2700
gtgcgggctc cccaggtgta cgtgctgccc cctccagagg aagagatgac caagaagcag 2760
gtcacactga cctgcatggt caccgacttc atgcccgagg acatctacgt ggaatggacc 2820
aacaatggca agaccgagct gaactacaag aacaccgagc ctgtgctgga ctccgacggc 2880
tcctacttca tgtactccaa gctgcgggtg gaaaagaaga actgggtcga gcggaactcc 2940
tactcctgct ccgtggtgca cgagggcctg cacaaccacc acaccaccaa gtccttctcc 3000
cggacccccg gcaaa 3015
<210> 36
<211> 2976
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 36
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
gaaccgaaat cttctgacaa aacccacacc tctccgccgt ctccggctcc ggaactgctg 2340
ggtggttctt ctgttttcat cttcccaccc aagatcaagg acgtgctgat gatctccctg 2400
tcccccatcg tgacctgcgt ggtggtggac gtgtccgagg acgaccccga cgtgcagatc 2460
agttggttcg tgaacaacgt ggaagtgcac accgcccaga cccagaccca cagagaggac 2520
tacaactcca ccctgcgggt ggtgtccgcc ctgcccatcc agcaccagga ctggatgtcc 2580
ggcaaagaat tcaagtgcaa agtgaacaac aaggacctgc ctgcccccat cgagcggacc 2640
atctccaagc ccaagggctc cgtgcgggct ccccaggtgt acgtgctgcc ccctccagag 2700
gaagagatga ccaagaagca ggtcacactg acctgcatgg tcaccgactt catgcccgag 2760
gacatctacg tggaatggac caacaatggc aagaccgagc tgaactacaa gaacaccgag 2820
cctgtgctgg actccgacgg ctcctacttc atgtactcca agctgcgggt ggaaaagaag 2880
aactgggtcg agcggaactc ctactcctgc tccgtggtgc acgagggcct gcacaaccac 2940
cacaccacca agtccttctc ccggaccccc ggcaaa 2976
<210> 37
<211> 996
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 37
Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala Gly Asp
1 5 10 15
Ser Leu Asp Cys Gly Gly Arg Gly Leu Ala Ala Leu Pro Gly Asp Leu
20 25 30
Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Lys Leu Ser Glu
35 40 45
Ile Asp Pro Ala Gly Phe Glu Asp Leu Pro Asn Leu Gln Glu Val Tyr
50 55 60
Leu Asn Asn Asn Glu Leu Thr Ala Val Pro Ser Leu Gly Ala Ala Ser
65 70 75 80
Ser His Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Arg Ser Val
85 90 95
Glu Gly Ser Gln Leu Lys Ala Tyr Leu Ser Leu Glu Val Leu Asp Leu
100 105 110
Ser Leu Asn Asn Ile Thr Glu Val Arg Asn Thr Cys Phe Pro His Gly
115 120 125
Pro Pro Ile Lys Glu Leu Asn Leu Ala Gly Asn Arg Ile Gly Thr Leu
130 135 140
Glu Leu Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr Leu Arg
145 150 155 160
Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Arg Ala Phe Lys Leu
165 170 175
Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg Leu Ile
180 185 190
Glu Gly Leu Thr Phe Gln Gly Leu Asn Ser Leu Glu Val Leu Lys Leu
195 200 205
Gln Arg Asn Asn Ile Ser Lys Leu Thr Asp Gly Ala Phe Trp Gly Leu
210 215 220
Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val Glu Val
225 230 235 240
Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu His Leu
245 250 255
Ser Asn Asn Ser Ile Ala Arg Ile His Arg Lys Gly Trp Ser Phe Cys
260 265 270
Gln Lys Leu His Glu Leu Val Leu Ser Phe Asn Asn Leu Thr Arg Leu
275 280 285
Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Val Leu Arg Leu
290 295 300
Ser His Asn Ser Ile Ser His Ile Ala Glu Gly Ala Phe Lys Gly Leu
305 310 315 320
Arg Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser Gly Thr
325 330 335
Ile Glu Asp Thr Ser Gly Ala Phe Ser Gly Leu Asp Ser Leu Ser Lys
340 345 350
Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg Ala Phe
355 360 365
Ser Gly Leu Glu Gly Leu Glu His Leu Asn Leu Gly Gly Asn Ala Ile
370 375 380
Arg Ser Val Gln Phe Asp Ala Phe Val Lys Met Lys Asn Leu Lys Glu
385 390 395 400
Leu His Ile Ser Ser Asp Ser Phe Leu Cys Asp Cys Gln Leu Lys Trp
405 410 415
Leu Pro Pro Trp Leu Ile Gly Arg Met Leu Gln Ala Phe Val Thr Ala
420 425 430
Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe Ser Val
435 440 445
Pro Pro Glu Ser Phe Val Cys Asp Asp Phe Leu Lys Pro Gln Ile Ile
450 455 460
Thr Gln Pro Glu Thr Thr Met Ala Met Val Gly Lys Asp Ile Arg Phe
465 470 475 480
Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe Ala Trp
485 490 495
Lys Lys Asp Asn Glu Val Leu Thr Asn Ala Asp Met Glu Asn Phe Val
500 505 510
His Val His Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr Ile Leu
515 520 525
His Leu Arg Gln Val Thr Phe Gly His Glu Gly Arg Tyr Gln Cys Val
530 535 540
Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg Leu Thr
545 550 555 560
Val Asn Val Leu Pro Ser Phe Thr Lys Thr Pro His Asp Ile Thr Ile
565 570 575
Arg Thr Thr Thr Val Ala Arg Leu Glu Cys Ala Ala Thr Gly His Pro
580 585 590
Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe Pro Ala
595 600 605
Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val Phe Phe
610 615 620
Ile Thr Asp Val Lys Ile Asp Asp Ala Gly Val Tyr Ser Cys Thr Ala
625 630 635 640
Gln Asn Ser Ala Gly Ser Ile Ser Ala Asn Ala Thr Leu Thr Val Leu
645 650 655
Glu Thr Pro Ser Leu Val Val Pro Leu Glu Asp Arg Val Val Ser Val
660 665 670
Gly Glu Thr Val Ala Leu Gln Cys Lys Ala Thr Gly Asn Pro Pro Pro
675 680 685
Arg Ile Thr Trp Phe Lys Gly Asp Arg Pro Leu Ser Leu Thr Glu Arg
690 695 700
His His Leu Thr Pro Asp Asn Gln Leu Leu Val Val Gln Asn Val Val
705 710 715 720
Ala Glu Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Thr Leu Gly
725 730 735
Thr Glu Arg Ala His Ser Gln Leu Ser Val Leu Pro Ala Ala Gly Cys
740 745 750
Arg Lys Asp Gly Thr Thr Val Gly Ile Phe Gly Ser Glu Pro Lys Ser
755 760 765
Ser Asp Lys Thr His Thr Ser Pro Pro Cys Pro Ala Pro Glu Leu Leu
770 775 780
Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu
785 790 795 800
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser
805 810 815
His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu
820 825 830
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr
835 840 845
Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn
850 855 860
Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
865 870 875 880
Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln
885 890 895
Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val
900 905 910
Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val
915 920 925
Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro
930 935 940
Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr
945 950 955 960
Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val
965 970 975
Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu
980 985 990
Ser Pro Gly Lys
995
<210> 38
<211> 997
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 38
Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala Gly Asp
1 5 10 15
Ser Leu Asp Cys Gly Gly Arg Gly Leu Ala Ala Leu Pro Gly Asp Leu
20 25 30
Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Lys Leu Ser Glu
35 40 45
Ile Asp Pro Ala Gly Phe Glu Asp Leu Pro Asn Leu Gln Glu Val Tyr
50 55 60
Leu Asn Asn Asn Glu Leu Thr Ala Val Pro Ser Leu Gly Ala Ala Ser
65 70 75 80
Ser His Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Arg Ser Val
85 90 95
Glu Gly Ser Gln Leu Lys Ala Tyr Leu Ser Leu Glu Val Leu Asp Leu
100 105 110
Ser Leu Asn Asn Ile Thr Glu Val Arg Asn Thr Cys Phe Pro His Gly
115 120 125
Pro Pro Ile Lys Glu Leu Asn Leu Ala Gly Asn Arg Ile Gly Thr Leu
130 135 140
Glu Leu Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr Leu Arg
145 150 155 160
Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Arg Ala Phe Lys Leu
165 170 175
Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg Leu Ile
180 185 190
Glu Gly Leu Thr Phe Gln Gly Leu Asn Ser Leu Glu Val Leu Lys Leu
195 200 205
Gln Arg Asn Asn Ile Ser Lys Leu Thr Asp Gly Ala Phe Trp Gly Leu
210 215 220
Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val Glu Val
225 230 235 240
Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu His Leu
245 250 255
Ser Asn Asn Ser Ile Ala Arg Ile His Arg Lys Gly Trp Ser Phe Cys
260 265 270
Gln Lys Leu His Glu Leu Val Leu Ser Phe Asn Asn Leu Thr Arg Leu
275 280 285
Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Val Leu Arg Leu
290 295 300
Ser His Asn Ser Ile Ser His Ile Ala Glu Gly Ala Phe Lys Gly Leu
305 310 315 320
Arg Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser Gly Thr
325 330 335
Ile Glu Asp Thr Ser Gly Ala Phe Ser Gly Leu Asp Ser Leu Ser Lys
340 345 350
Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg Ala Phe
355 360 365
Ser Gly Leu Glu Gly Leu Glu His Leu Asn Leu Gly Gly Asn Ala Ile
370 375 380
Arg Ser Val Gln Phe Asp Ala Phe Val Lys Met Lys Asn Leu Lys Glu
385 390 395 400
Leu His Ile Ser Ser Asp Ser Phe Leu Cys Asp Cys Gln Leu Lys Trp
405 410 415
Leu Pro Pro Trp Leu Ile Gly Arg Met Leu Gln Ala Phe Val Thr Ala
420 425 430
Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe Ser Val
435 440 445
Pro Pro Glu Ser Phe Val Cys Asp Asp Phe Leu Lys Pro Gln Ile Ile
450 455 460
Thr Gln Pro Glu Thr Thr Met Ala Met Val Gly Lys Asp Ile Arg Phe
465 470 475 480
Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe Ala Trp
485 490 495
Lys Lys Asp Asn Glu Val Leu Thr Asn Ala Asp Met Glu Asn Phe Val
500 505 510
His Val His Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr Ile Leu
515 520 525
His Leu Arg Gln Val Thr Phe Gly His Glu Gly Arg Tyr Gln Cys Val
530 535 540
Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg Leu Thr
545 550 555 560
Val Asn Val Leu Pro Ser Phe Thr Lys Thr Pro His Asp Ile Thr Ile
565 570 575
Arg Thr Thr Thr Val Ala Arg Leu Glu Cys Ala Ala Thr Gly His Pro
580 585 590
Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe Pro Ala
595 600 605
Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val Phe Phe
610 615 620
Ile Thr Asp Val Lys Ile Asp Asp Ala Gly Val Tyr Ser Cys Thr Ala
625 630 635 640
Gln Asn Ser Ala Gly Ser Ile Ser Ala Asn Ala Thr Leu Thr Val Leu
645 650 655
Glu Thr Pro Ser Leu Val Val Pro Leu Glu Asp Arg Val Val Ser Val
660 665 670
Gly Glu Thr Val Ala Leu Gln Cys Lys Ala Thr Gly Asn Pro Pro Pro
675 680 685
Arg Ile Thr Trp Phe Lys Gly Asp Arg Pro Leu Ser Leu Thr Glu Arg
690 695 700
His His Leu Thr Pro Asp Asn Gln Leu Leu Val Val Gln Asn Val Val
705 710 715 720
Ala Glu Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Thr Leu Gly
725 730 735
Thr Glu Arg Ala His Ser Gln Leu Ser Val Leu Pro Ala Ala Gly Cys
740 745 750
Arg Lys Asp Gly Thr Thr Val Gly Ile Phe Gly Ser Glu Pro Arg Gly
755 760 765
Pro Thr Ile Lys Pro Cys Pro Pro Cys Lys Cys Pro Ala Pro Asn Leu
770 775 780
Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
785 790 795 800
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
805 810 815
Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val
820 825 830
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser
835 840 845
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
850 855 860
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala
865 870 875 880
Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
885 890 895
Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln
900 905 910
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
915 920 925
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
930 935 940
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu
945 950 955 960
Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser
965 970 975
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
980 985 990
Leu Ser Pro Gly Lys
995
<210> 39
<211> 1009
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 39
Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala Gly Asp
1 5 10 15
Ser Leu Asp Cys Gly Gly Arg Gly Leu Ala Ala Leu Pro Gly Asp Leu
20 25 30
Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Lys Leu Ser Glu
35 40 45
Ile Asp Pro Ala Gly Phe Glu Asp Leu Pro Asn Leu Gln Glu Val Tyr
50 55 60
Leu Asn Asn Asn Glu Leu Thr Ala Val Pro Ser Leu Gly Ala Ala Ser
65 70 75 80
Ser His Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Arg Ser Val
85 90 95
Glu Gly Ser Gln Leu Lys Ala Tyr Leu Ser Leu Glu Val Leu Asp Leu
100 105 110
Ser Leu Asn Asn Ile Thr Glu Val Arg Asn Thr Cys Phe Pro His Gly
115 120 125
Pro Pro Ile Lys Glu Leu Asn Leu Ala Gly Asn Arg Ile Gly Thr Leu
130 135 140
Glu Leu Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr Leu Arg
145 150 155 160
Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Arg Ala Phe Lys Leu
165 170 175
Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg Leu Ile
180 185 190
Glu Gly Leu Thr Phe Gln Gly Leu Asn Ser Leu Glu Val Leu Lys Leu
195 200 205
Gln Arg Asn Asn Ile Ser Lys Leu Thr Asp Gly Ala Phe Trp Gly Leu
210 215 220
Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val Glu Val
225 230 235 240
Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu His Leu
245 250 255
Ser Asn Asn Ser Ile Ala Arg Ile His Arg Lys Gly Trp Ser Phe Cys
260 265 270
Gln Lys Leu His Glu Leu Val Leu Ser Phe Asn Asn Leu Thr Arg Leu
275 280 285
Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Val Leu Arg Leu
290 295 300
Ser His Asn Ser Ile Ser His Ile Ala Glu Gly Ala Phe Lys Gly Leu
305 310 315 320
Arg Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser Gly Thr
325 330 335
Ile Glu Asp Thr Ser Gly Ala Phe Ser Gly Leu Asp Ser Leu Ser Lys
340 345 350
Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg Ala Phe
355 360 365
Ser Gly Leu Glu Gly Leu Glu His Leu Asn Leu Gly Gly Asn Ala Ile
370 375 380
Arg Ser Val Gln Phe Asp Ala Phe Val Lys Met Lys Asn Leu Lys Glu
385 390 395 400
Leu His Ile Ser Ser Asp Ser Phe Leu Cys Asp Cys Gln Leu Lys Trp
405 410 415
Leu Pro Pro Trp Leu Ile Gly Arg Met Leu Gln Ala Phe Val Thr Ala
420 425 430
Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe Ser Val
435 440 445
Pro Pro Glu Ser Phe Val Cys Asp Asp Phe Leu Lys Pro Gln Ile Ile
450 455 460
Thr Gln Pro Glu Thr Thr Met Ala Met Val Gly Lys Asp Ile Arg Phe
465 470 475 480
Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe Ala Trp
485 490 495
Lys Lys Asp Asn Glu Val Leu Thr Asn Ala Asp Met Glu Asn Phe Val
500 505 510
His Val His Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr Ile Leu
515 520 525
His Leu Arg Gln Val Thr Phe Gly His Glu Gly Arg Tyr Gln Cys Val
530 535 540
Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg Leu Thr
545 550 555 560
Val Asn Val Leu Pro Ser Phe Thr Lys Thr Pro His Asp Ile Thr Ile
565 570 575
Arg Thr Thr Thr Val Ala Arg Leu Glu Cys Ala Ala Thr Gly His Pro
580 585 590
Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe Pro Ala
595 600 605
Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val Phe Phe
610 615 620
Ile Thr Asp Val Lys Ile Asp Asp Ala Gly Val Tyr Ser Cys Thr Ala
625 630 635 640
Gln Asn Ser Ala Gly Ser Ile Ser Ala Asn Ala Thr Leu Thr Val Leu
645 650 655
Glu Thr Pro Ser Leu Val Val Pro Leu Glu Asp Arg Val Val Ser Val
660 665 670
Gly Glu Thr Val Ala Leu Gln Cys Lys Ala Thr Gly Asn Pro Pro Pro
675 680 685
Arg Ile Thr Trp Phe Lys Gly Asp Arg Pro Leu Ser Leu Thr Glu Arg
690 695 700
His His Leu Thr Pro Asp Asn Gln Leu Leu Val Val Gln Asn Val Val
705 710 715 720
Ala Glu Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Thr Leu Gly
725 730 735
Thr Glu Arg Ala His Ser Gln Leu Ser Val Leu Pro Ala Ala Gly Cys
740 745 750
Arg Lys Asp Gly Thr Thr Val Gly Ile Phe Gly Ser Arg Asn Thr Gly
755 760 765
Arg Gly Gly Glu Glu Lys Lys Lys Glu Lys Glu Lys Glu Glu Gln Glu
770 775 780
Glu Arg Glu Thr Lys Thr Pro Glu Cys Pro Ser His Thr Gln Pro Leu
785 790 795 800
Gly Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser
805 810 815
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp
820 825 830
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn
835 840 845
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val
850 855 860
Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu
865 870 875 880
Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys
885 890 895
Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr
900 905 910
Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr
915 920 925
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu
930 935 940
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu
945 950 955 960
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys
965 970 975
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu
980 985 990
Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
995 1000 1005
Lys
<210> 40
<211> 996
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 40
Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala Gly Asp
1 5 10 15
Ser Leu Asp Cys Gly Gly Arg Gly Leu Ala Ala Leu Pro Gly Asp Leu
20 25 30
Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Lys Leu Ser Glu
35 40 45
Ile Asp Pro Ala Gly Phe Glu Asp Leu Pro Asn Leu Gln Glu Val Tyr
50 55 60
Leu Asn Asn Asn Glu Leu Thr Ala Val Pro Ser Leu Gly Ala Ala Ser
65 70 75 80
Ser His Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Arg Ser Val
85 90 95
Glu Gly Ser Gln Leu Lys Ala Tyr Leu Ser Leu Glu Val Leu Asp Leu
100 105 110
Ser Leu Asn Asn Ile Thr Glu Val Arg Asn Thr Cys Phe Pro His Gly
115 120 125
Pro Pro Ile Lys Glu Leu Asn Leu Ala Gly Asn Arg Ile Gly Thr Leu
130 135 140
Glu Leu Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr Leu Arg
145 150 155 160
Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Arg Ala Phe Lys Leu
165 170 175
Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg Leu Ile
180 185 190
Glu Gly Leu Thr Phe Gln Gly Leu Asn Ser Leu Glu Val Leu Lys Leu
195 200 205
Gln Arg Asn Asn Ile Ser Lys Leu Thr Asp Gly Ala Phe Trp Gly Leu
210 215 220
Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val Glu Val
225 230 235 240
Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu His Leu
245 250 255
Ser Asn Asn Ser Ile Ala Arg Ile His Arg Lys Gly Trp Ser Phe Cys
260 265 270
Gln Lys Leu His Glu Leu Val Leu Ser Phe Asn Asn Leu Thr Arg Leu
275 280 285
Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Val Leu Arg Leu
290 295 300
Ser His Asn Ser Ile Ser His Ile Ala Glu Gly Ala Phe Lys Gly Leu
305 310 315 320
Arg Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser Gly Thr
325 330 335
Ile Glu Asp Thr Ser Gly Ala Phe Ser Gly Leu Asp Ser Leu Ser Lys
340 345 350
Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg Ala Phe
355 360 365
Ser Gly Leu Glu Gly Leu Glu His Leu Asn Leu Gly Gly Asn Ala Ile
370 375 380
Arg Ser Val Gln Phe Asp Ala Phe Val Lys Met Lys Asn Leu Lys Glu
385 390 395 400
Leu His Ile Ser Ser Asp Ser Phe Leu Cys Asp Cys Gln Leu Lys Trp
405 410 415
Leu Pro Pro Trp Leu Ile Gly Arg Met Leu Gln Ala Phe Val Thr Ala
420 425 430
Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe Ser Val
435 440 445
Pro Pro Glu Ser Phe Val Cys Asp Asp Phe Leu Lys Pro Gln Ile Ile
450 455 460
Thr Gln Pro Glu Thr Thr Met Ala Met Val Gly Lys Asp Ile Arg Phe
465 470 475 480
Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe Ala Trp
485 490 495
Lys Lys Asp Asn Glu Val Leu Thr Asn Ala Asp Met Glu Asn Phe Val
500 505 510
His Val His Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr Ile Leu
515 520 525
His Leu Arg Gln Val Thr Phe Gly His Glu Gly Arg Tyr Gln Cys Val
530 535 540
Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg Leu Thr
545 550 555 560
Val Asn Val Leu Pro Ser Phe Thr Lys Thr Pro His Asp Ile Thr Ile
565 570 575
Arg Thr Thr Thr Val Ala Arg Leu Glu Cys Ala Ala Thr Gly His Pro
580 585 590
Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe Pro Ala
595 600 605
Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val Phe Phe
610 615 620
Ile Thr Asp Val Lys Ile Asp Asp Ala Gly Val Tyr Ser Cys Thr Ala
625 630 635 640
Gln Asn Ser Ala Gly Ser Ile Ser Ala Asn Ala Thr Leu Thr Val Leu
645 650 655
Glu Thr Pro Ser Leu Val Val Pro Leu Glu Asp Arg Val Val Ser Val
660 665 670
Gly Glu Thr Val Ala Leu Gln Cys Lys Ala Thr Gly Asn Pro Pro Pro
675 680 685
Arg Ile Thr Trp Phe Lys Gly Asp Arg Pro Leu Ser Leu Thr Glu Arg
690 695 700
His His Leu Thr Pro Asp Asn Gln Leu Leu Val Val Gln Asn Val Val
705 710 715 720
Ala Glu Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Thr Leu Gly
725 730 735
Thr Glu Arg Ala His Ser Gln Leu Ser Val Leu Pro Ala Ala Gly Cys
740 745 750
Arg Lys Asp Gly Thr Thr Val Gly Ile Phe Gly Ser Glu Pro Lys Ser
755 760 765
Ser Asp Lys Thr His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu
770 775 780
Gly Gly Ser Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu
785 790 795 800
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser
805 810 815
His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu
820 825 830
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr
835 840 845
Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn
850 855 860
Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
865 870 875 880
Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln
885 890 895
Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val
900 905 910
Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val
915 920 925
Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro
930 935 940
Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr
945 950 955 960
Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val
965 970 975
Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu
980 985 990
Ser Pro Gly Lys
995
<210> 41
<211> 2988
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 41
gctgggcctc gggctccttg tgctgccgcc tgcacatgtg caggcgattc cctggactgc 60
ggcggcagag gcctggccgc cctgcctggc gatctgccat cctggacccg gagcctgaac 120
ctgagctaca acaagctgag cgagatcgat cccgccggct ttgaggacct gcctaacctg 180
caggaggtgt atctgaacaa taacgagctg accgcggtac catccctggg cgctgcttca 240
tcacatgtcg tctctctctt tctgcagcac aacaagattc gcagcgtgga ggggagccag 300
ctgaaggcct acctttcctt agaagtgtta gatctgagtt tgaacaacat cacggaagtg 360
cggaacacct gctttccaca cggaccgcct ataaaggagc tcaacctggc aggcaatcgg 420
attggcaccc tggagttggg agcatttgat ggtctgtcac ggtcgctgct aactcttcgc 480
ctgagcaaaa acaggatcac ccagcttcct gtaagagcat tcaagctacc caggctgaca 540
caactggacc tcaatcggaa caggattcgg ctgatagagg gcctcacctt ccaggggctc 600
aacagcttgg aggtgctgaa gcttcagcga aacaacatca gcaaactgac agatggggcc 660
ttctggggac tgtccaagat gcatgtgctg cacctggagt acaacagcct ggtagaagtg 720
aacagcggct cgctctacgg cctcacggcc ctgcatcagc tccacctcag caacaattcc 780
atcgctcgca ttcaccgcaa gggctggagc ttctgccaga agctgcatga gttggtcctg 840
tccttcaaca acctgacacg gctggacgag gagagcctgg ccgagctgag cagcctgagt 900
gtcctgcgtc tcagccacaa ttccatcagc cacattgcgg agggtgcctt caagggactc 960
aggagcctgc gagtcttgga tctggaccat aacgagattt cgggcacaat agaggacacg 1020
agcggcgcct tctcagggct cgacagcctc agcaagctga ctctgtttgg aaacaagatc 1080
aagtctgtgg ctaagagagc attctcgggg ctggaaggcc tggagcacct gaaccttgga 1140
gggaatgcga tcagatctgt ccagtttgat gcctttgtga agatgaagaa tcttaaagag 1200
ctccatatca gcagcgacag cttcctgtgt gactgccagc tgaagtggct gcccccgtgg 1260
ctaattggca ggatgctgca ggcctttgtg acagccacct gtgcccaccc agaatcactg 1320
aagggtcaga gcattttctc tgtgccacca gagagtttcg tgtgcgatga cttcctgaag 1380
ccacagatca tcacccagcc agaaaccacc atggctatgg tgggcaagga catccggttt 1440
acatgctcag cagccagcag cagcagctcc cccatgacct ttgcctggaa gaaagacaat 1500
gaagtcctga ccaatgcaga catggagaac tttgtccacg tccacgcgca ggacggggaa 1560
gtgatggagt acaccaccat cctgcacctc cgtcaggtca ctttcgggca cgagggccgc 1620
taccaatgtg tcatcaccaa ccactttggc tccacctatt cacataaggc caggctcacc 1680
gtgaatgtgt tgccatcatt caccaaaacg ccccacgaca taaccatccg gaccaccacc 1740
gtggcccgcc tcgaatgtgc tgccacaggt cacccaaacc ctcagattgc ctggcagaag 1800
gatggaggca cggatttccc cgctgcccgt gagcgacgca tgcatgtcat gccggatgac 1860
gacgtgtttt tcatcactga tgtgaaaata gatgacgcag gggtttacag ctgtactgct 1920
cagaactcag ccggttctat ttcagctaat gccaccctga ctgtcctaga gaccccatcc 1980
ttggtggtcc ccttggaaga ccgtgtggta tctgtgggag aaacagtggc cctccaatgc 2040
aaagccacgg ggaaccctcc gccccgcatc acctggttca agggggaccg cccgctgagc 2100
ctcactgagc ggcaccacct gacccctgac aaccagctcc tggtggttca gaacgtggtg 2160
gcagaggatg cgggccgata tacctgtgag atgtccaaca ccctgggcac ggagcgagct 2220
cacagccagc tgagcgtcct gcccgcagca ggctgcagga aggatgggac cacggtaggc 2280
atcttcggat ccgagccaaa gtcctctgat aagacacaca cctctccacc atgcccagca 2340
ccagagctgc tgggaggacc aagcgtgttc ctgtttcctc caaagcccaa ggacacactg 2400
atgatctcca ggacaccaga ggtgacctgc gtggtggtgg acgtgagcca cgaggacccc 2460
gaggtgaagt tcaactggta cgtggatggc gtggaggtgc acaatgccaa gaccaagccc 2520
agagaggagc agtacaactc tacctatagg gtggtgagcg tgctgacagt gctgcaccag 2580
gactggctga acggcaagga gtataagtgc aaggtgagca ataaggccct gcctgcccca 2640
atcgagaaga caatctccaa ggccaagggc cagccaagag agccccaggt gtacaccctg 2700
ccccctagca gggatgagct gacaaagaac caggtgtccc tgacctgtct ggtgaagggc 2760
ttttatccct ccgacatcgc cgtggagtgg gagtctaatg gccagcctga gaataactac 2820
aagacaaccc cacccgtgct ggattctgac ggcagcttct ttctgtattc taagctgacc 2880
gtggacaaga gcaggtggca gcagggcaac gtgttcagct gctccgtgat gcacgaagca 2940
ctgcacaatc actacaccca gaaatcactg tcactgagcc ctggcaaa 2988
<210> 42
<211> 2991
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 42
gctgggcctc gggctccttg tgctgccgcc tgcacatgtg caggcgattc cctggactgc 60
ggcggcagag gcctggccgc cctgcctggc gatctgccat cctggacccg gagcctgaac 120
ctgagctaca acaagctgag cgagatcgat cccgccggct ttgaggacct gcctaacctg 180
caggaggtgt atctgaacaa taacgagctg accgcggtac catccctggg cgctgcttca 240
tcacatgtcg tctctctctt tctgcagcac aacaagattc gcagcgtgga ggggagccag 300
ctgaaggcct acctttcctt agaagtgtta gatctgagtt tgaacaacat cacggaagtg 360
cggaacacct gctttccaca cggaccgcct ataaaggagc tcaacctggc aggcaatcgg 420
attggcaccc tggagttggg agcatttgat ggtctgtcac ggtcgctgct aactcttcgc 480
ctgagcaaaa acaggatcac ccagcttcct gtaagagcat tcaagctacc caggctgaca 540
caactggacc tcaatcggaa caggattcgg ctgatagagg gcctcacctt ccaggggctc 600
aacagcttgg aggtgctgaa gcttcagcga aacaacatca gcaaactgac agatggggcc 660
ttctggggac tgtccaagat gcatgtgctg cacctggagt acaacagcct ggtagaagtg 720
aacagcggct cgctctacgg cctcacggcc ctgcatcagc tccacctcag caacaattcc 780
atcgctcgca ttcaccgcaa gggctggagc ttctgccaga agctgcatga gttggtcctg 840
tccttcaaca acctgacacg gctggacgag gagagcctgg ccgagctgag cagcctgagt 900
gtcctgcgtc tcagccacaa ttccatcagc cacattgcgg agggtgcctt caagggactc 960
aggagcctgc gagtcttgga tctggaccat aacgagattt cgggcacaat agaggacacg 1020
agcggcgcct tctcagggct cgacagcctc agcaagctga ctctgtttgg aaacaagatc 1080
aagtctgtgg ctaagagagc attctcgggg ctggaaggcc tggagcacct gaaccttgga 1140
gggaatgcga tcagatctgt ccagtttgat gcctttgtga agatgaagaa tcttaaagag 1200
ctccatatca gcagcgacag cttcctgtgt gactgccagc tgaagtggct gcccccgtgg 1260
ctaattggca ggatgctgca ggcctttgtg acagccacct gtgcccaccc agaatcactg 1320
aagggtcaga gcattttctc tgtgccacca gagagtttcg tgtgcgatga cttcctgaag 1380
ccacagatca tcacccagcc agaaaccacc atggctatgg tgggcaagga catccggttt 1440
acatgctcag cagccagcag cagcagctcc cccatgacct ttgcctggaa gaaagacaat 1500
gaagtcctga ccaatgcaga catggagaac tttgtccacg tccacgcgca ggacggggaa 1560
gtgatggagt acaccaccat cctgcacctc cgtcaggtca ctttcgggca cgagggccgc 1620
taccaatgtg tcatcaccaa ccactttggc tccacctatt cacataaggc caggctcacc 1680
gtgaatgtgt tgccatcatt caccaaaacg ccccacgaca taaccatccg gaccaccacc 1740
gtggcccgcc tcgaatgtgc tgccacaggt cacccaaacc ctcagattgc ctggcagaag 1800
gatggaggca cggatttccc cgctgcccgt gagcgacgca tgcatgtcat gccggatgac 1860
gacgtgtttt tcatcactga tgtgaaaata gatgacgcag gggtttacag ctgtactgct 1920
cagaactcag ccggttctat ttcagctaat gccaccctga ctgtcctaga gaccccatcc 1980
ttggtggtcc ccttggaaga ccgtgtggta tctgtgggag aaacagtggc cctccaatgc 2040
aaagccacgg ggaaccctcc gccccgcatc acctggttca agggggaccg cccgctgagc 2100
ctcactgagc ggcaccacct gacccctgac aaccagctcc tggtggttca gaacgtggtg 2160
gcagaggatg cgggccgata tacctgtgag atgtccaaca ccctgggcac ggagcgagct 2220
cacagccagc tgagcgtcct gcccgcagca ggctgcagga aggatgggac cacggtaggc 2280
atcttcggat ccgagcctcg gggccctacc atcaagccct gccccccttg caagtgccct 2340
gcccctaatc tgctgggcgg accctccgtg ttcctgtttc ctccaaagcc caaggacaca 2400
ctgatgatct ccaggacacc agaggtgacc tgcgtggtgg tggacgtgag ccacgaggac 2460
cccgaggtga agttcaactg gtacgtggat ggcgtggagg tgcacaatgc caagaccaag 2520
cccagagagg agcagtacaa ctctacctat agggtggtga gcgtgctgac agtgctgcac 2580
caggactggc tgaacggcaa ggagtataag tgcaaggtga gcaataaggc cctgcctgcc 2640
ccaatcgaga agacaatctc caaggccaag ggccagccaa gagagcccca ggtgtacacc 2700
ctgcccccta gcagggatga gctgacaaag aaccaggtgt ccctgacctg tctggtgaag 2760
ggcttttatc cctccgacat cgccgtggag tgggagtcta atggccagcc tgagaataac 2820
tacaagacaa ccccacccgt gctggattct gacggcagct tctttctgta ttctaagctg 2880
accgtggaca agagcaggtg gcagcagggc aacgtgttca gctgctccgt gatgcacgaa 2940
gcactgcaca atcactacac ccagaaatca ctgtcactga gccctggcaa a 2991
<210> 43
<211> 3027
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 43
gctgggcctc gggctccttg tgctgccgcc tgcacatgtg caggcgattc cctggactgc 60
ggcggcagag gcctggccgc cctgcctggc gatctgccat cctggacccg gagcctgaac 120
ctgagctaca acaagctgag cgagatcgat cccgccggct ttgaggacct gcctaacctg 180
caggaggtgt atctgaacaa taacgagctg accgcggtac catccctggg cgctgcttca 240
tcacatgtcg tctctctctt tctgcagcac aacaagattc gcagcgtgga ggggagccag 300
ctgaaggcct acctttcctt agaagtgtta gatctgagtt tgaacaacat cacggaagtg 360
cggaacacct gctttccaca cggaccgcct ataaaggagc tcaacctggc aggcaatcgg 420
attggcaccc tggagttggg agcatttgat ggtctgtcac ggtcgctgct aactcttcgc 480
ctgagcaaaa acaggatcac ccagcttcct gtaagagcat tcaagctacc caggctgaca 540
caactggacc tcaatcggaa caggattcgg ctgatagagg gcctcacctt ccaggggctc 600
aacagcttgg aggtgctgaa gcttcagcga aacaacatca gcaaactgac agatggggcc 660
ttctggggac tgtccaagat gcatgtgctg cacctggagt acaacagcct ggtagaagtg 720
aacagcggct cgctctacgg cctcacggcc ctgcatcagc tccacctcag caacaattcc 780
atcgctcgca ttcaccgcaa gggctggagc ttctgccaga agctgcatga gttggtcctg 840
tccttcaaca acctgacacg gctggacgag gagagcctgg ccgagctgag cagcctgagt 900
gtcctgcgtc tcagccacaa ttccatcagc cacattgcgg agggtgcctt caagggactc 960
aggagcctgc gagtcttgga tctggaccat aacgagattt cgggcacaat agaggacacg 1020
agcggcgcct tctcagggct cgacagcctc agcaagctga ctctgtttgg aaacaagatc 1080
aagtctgtgg ctaagagagc attctcgggg ctggaaggcc tggagcacct gaaccttgga 1140
gggaatgcga tcagatctgt ccagtttgat gcctttgtga agatgaagaa tcttaaagag 1200
ctccatatca gcagcgacag cttcctgtgt gactgccagc tgaagtggct gcccccgtgg 1260
ctaattggca ggatgctgca ggcctttgtg acagccacct gtgcccaccc agaatcactg 1320
aagggtcaga gcattttctc tgtgccacca gagagtttcg tgtgcgatga cttcctgaag 1380
ccacagatca tcacccagcc agaaaccacc atggctatgg tgggcaagga catccggttt 1440
acatgctcag cagccagcag cagcagctcc cccatgacct ttgcctggaa gaaagacaat 1500
gaagtcctga ccaatgcaga catggagaac tttgtccacg tccacgcgca ggacggggaa 1560
gtgatggagt acaccaccat cctgcacctc cgtcaggtca ctttcgggca cgagggccgc 1620
taccaatgtg tcatcaccaa ccactttggc tccacctatt cacataaggc caggctcacc 1680
gtgaatgtgt tgccatcatt caccaaaacg ccccacgaca taaccatccg gaccaccacc 1740
gtggcccgcc tcgaatgtgc tgccacaggt cacccaaacc ctcagattgc ctggcagaag 1800
gatggaggca cggatttccc cgctgcccgt gagcgacgca tgcatgtcat gccggatgac 1860
gacgtgtttt tcatcactga tgtgaaaata gatgacgcag gggtttacag ctgtactgct 1920
cagaactcag ccggttctat ttcagctaat gccaccctga ctgtcctaga gaccccatcc 1980
ttggtggtcc ccttggaaga ccgtgtggta tctgtgggag aaacagtggc cctccaatgc 2040
aaagccacgg ggaaccctcc gccccgcatc acctggttca agggggaccg cccgctgagc 2100
ctcactgagc ggcaccacct gacccctgac aaccagctcc tggtggttca gaacgtggtg 2160
gcagaggatg cgggccgata tacctgtgag atgtccaaca ccctgggcac ggagcgagct 2220
cacagccagc tgagcgtcct gcccgcagca ggctgcagga aggatgggac cacggtaggc 2280
atcttcggat cccgcaacac cggccgcggc ggcgaggaga agaagaagga gaaggagaag 2340
gaggagcagg aggagcgcga gaccaagacc cccgagtgcc ccagccacac ccagcccctg 2400
ggcgtgttcc tgtttcctcc aaagcccaag gacacactga tgatctccag gacaccagag 2460
gtgacctgcg tggtggtgga cgtgagccac gaggaccccg aggtgaagtt caactggtac 2520
gtggatggcg tggaggtgca caatgccaag accaagccca gagaggagca gtacaactct 2580
acctataggg tggtgagcgt gctgacagtg ctgcaccagg actggctgaa cggcaaggag 2640
tataagtgca aggtgagcaa taaggccctg cctgccccaa tcgagaagac aatctccaag 2700
gccaagggcc agccaagaga gccccaggtg tacaccctgc cccctagcag ggatgagctg 2760
acaaagaacc aggtgtccct gacctgtctg gtgaagggct tttatccctc cgacatcgcc 2820
gtggagtggg agtctaatgg ccagcctgag aataactaca agacaacccc acccgtgctg 2880
gattctgacg gcagcttctt tctgtattct aagctgaccg tggacaagag caggtggcag 2940
cagggcaacg tgttcagctg ctccgtgatg cacgaagcac tgcacaatca ctacacccag 3000
aaatcactgt cactgagccc tggcaaa 3027
<210> 44
<211> 2988
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 44
gctgggcctc gggctccttg tgctgccgcc tgcacatgtg caggcgattc cctggactgc 60
ggcggcagag gcctggccgc cctgcctggc gatctgccat cctggacccg gagcctgaac 120
ctgagctaca acaagctgag cgagatcgat cccgccggct ttgaggacct gcctaacctg 180
caggaggtgt atctgaacaa taacgagctg accgcggtac catccctggg cgctgcttca 240
tcacatgtcg tctctctctt tctgcagcac aacaagattc gcagcgtgga ggggagccag 300
ctgaaggcct acctttcctt agaagtgtta gatctgagtt tgaacaacat cacggaagtg 360
cggaacacct gctttccaca cggaccgcct ataaaggagc tcaacctggc aggcaatcgg 420
attggcaccc tggagttggg agcatttgat ggtctgtcac ggtcgctgct aactcttcgc 480
ctgagcaaaa acaggatcac ccagcttcct gtaagagcat tcaagctacc caggctgaca 540
caactggacc tcaatcggaa caggattcgg ctgatagagg gcctcacctt ccaggggctc 600
aacagcttgg aggtgctgaa gcttcagcga aacaacatca gcaaactgac agatggggcc 660
ttctggggac tgtccaagat gcatgtgctg cacctggagt acaacagcct ggtagaagtg 720
aacagcggct cgctctacgg cctcacggcc ctgcatcagc tccacctcag caacaattcc 780
atcgctcgca ttcaccgcaa gggctggagc ttctgccaga agctgcatga gttggtcctg 840
tccttcaaca acctgacacg gctggacgag gagagcctgg ccgagctgag cagcctgagt 900
gtcctgcgtc tcagccacaa ttccatcagc cacattgcgg agggtgcctt caagggactc 960
aggagcctgc gagtcttgga tctggaccat aacgagattt cgggcacaat agaggacacg 1020
agcggcgcct tctcagggct cgacagcctc agcaagctga ctctgtttgg aaacaagatc 1080
aagtctgtgg ctaagagagc attctcgggg ctggaaggcc tggagcacct gaaccttgga 1140
gggaatgcga tcagatctgt ccagtttgat gcctttgtga agatgaagaa tcttaaagag 1200
ctccatatca gcagcgacag cttcctgtgt gactgccagc tgaagtggct gcccccgtgg 1260
ctaattggca ggatgctgca ggcctttgtg acagccacct gtgcccaccc agaatcactg 1320
aagggtcaga gcattttctc tgtgccacca gagagtttcg tgtgcgatga cttcctgaag 1380
ccacagatca tcacccagcc agaaaccacc atggctatgg tgggcaagga catccggttt 1440
acatgctcag cagccagcag cagcagctcc cccatgacct ttgcctggaa gaaagacaat 1500
gaagtcctga ccaatgcaga catggagaac tttgtccacg tccacgcgca ggacggggaa 1560
gtgatggagt acaccaccat cctgcacctc cgtcaggtca ctttcgggca cgagggccgc 1620
taccaatgtg tcatcaccaa ccactttggc tccacctatt cacataaggc caggctcacc 1680
gtgaatgtgt tgccatcatt caccaaaacg ccccacgaca taaccatccg gaccaccacc 1740
gtggcccgcc tcgaatgtgc tgccacaggt cacccaaacc ctcagattgc ctggcagaag 1800
gatggaggca cggatttccc cgctgcccgt gagcgacgca tgcatgtcat gccggatgac 1860
gacgtgtttt tcatcactga tgtgaaaata gatgacgcag gggtttacag ctgtactgct 1920
cagaactcag ccggttctat ttcagctaat gccaccctga ctgtcctaga gaccccatcc 1980
ttggtggtcc ccttggaaga ccgtgtggta tctgtgggag aaacagtggc cctccaatgc 2040
aaagccacgg ggaaccctcc gccccgcatc acctggttca agggggaccg cccgctgagc 2100
ctcactgagc ggcaccacct gacccctgac aaccagctcc tggtggttca gaacgtggtg 2160
gcagaggatg cgggccgata tacctgtgag atgtccaaca ccctgggcac ggagcgagct 2220
cacagccagc tgagcgtcct gcccgcagca ggctgcagga aggatgggac cacggtaggc 2280
atcttcggat ccgaaccgaa atcttctgac aaaacccaca cctctccgcc gtctccggct 2340
ccggaactgc tgggtggttc ttctgttttc ctgtttcctc caaagcccaa ggacacactg 2400
atgatctcca ggacaccaga ggtgacctgc gtggtggtgg acgtgagcca cgaggacccc 2460
gaggtgaagt tcaactggta cgtggatggc gtggaggtgc acaatgccaa gaccaagccc 2520
agagaggagc agtacaactc tacctatagg gtggtgagcg tgctgacagt gctgcaccag 2580
gactggctga acggcaagga gtataagtgc aaggtgagca ataaggccct gcctgcccca 2640
atcgagaaga caatctccaa ggccaagggc cagccaagag agccccaggt gtacaccctg 2700
ccccctagca gggatgagct gacaaagaac caggtgtccc tgacctgtct ggtgaagggc 2760
ttttatccct ccgacatcgc cgtggagtgg gagtctaatg gccagcctga gaataactac 2820
aagacaaccc cacccgtgct ggattctgac ggcagcttct ttctgtattc taagctgacc 2880
gtggacaaga gcaggtggca gcagggcaac gtgttcagct gctccgtgat gcacgaagca 2940
ctgcacaatc actacaccca gaaatcactg tcactgagcc ctggcaaa 2988
<210> 45
<211> 994
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 45
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Gly Ser Glu Pro Lys Ser Ser Asp
755 760 765
Lys Thr His Thr Ser Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly
770 775 780
Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile
785 790 795 800
Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu
805 810 815
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
820 825 830
Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg
835 840 845
Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
850 855 860
Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu
865 870 875 880
Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr
885 890 895
Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu
900 905 910
Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp
915 920 925
Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val
930 935 940
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp
945 950 955 960
Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His
965 970 975
Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro
980 985 990
Gly Lys
<210> 46
<211> 995
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 46
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Gly Ser Glu Pro Arg Gly Pro Thr
755 760 765
Ile Lys Pro Cys Pro Pro Cys Lys Cys Pro Ala Pro Asn Leu Leu Gly
770 775 780
Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met
785 790 795 800
Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His
805 810 815
Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val
820 825 830
His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr
835 840 845
Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly
850 855 860
Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile
865 870 875 880
Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val
885 890 895
Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser
900 905 910
Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu
915 920 925
Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro
930 935 940
Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val
945 950 955 960
Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met
965 970 975
His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser
980 985 990
Pro Gly Lys
995
<210> 47
<211> 1007
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 47
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Gly Ser Arg Asn Thr Gly Arg Gly
755 760 765
Gly Glu Glu Lys Lys Lys Glu Lys Glu Lys Glu Glu Gln Glu Glu Arg
770 775 780
Glu Thr Lys Thr Pro Glu Cys Pro Ser His Thr Gln Pro Leu Gly Val
785 790 795 800
Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr
805 810 815
Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu
820 825 830
Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys
835 840 845
Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser
850 855 860
Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys
865 870 875 880
Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile
885 890 895
Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro
900 905 910
Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu
915 920 925
Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn
930 935 940
Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser
945 950 955 960
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg
965 970 975
Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu
980 985 990
His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
995 1000 1005
<210> 48
<211> 994
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 48
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Gly Ser Glu Pro Lys Ser Ser Asp
755 760 765
Lys Thr His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly
770 775 780
Ser Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile
785 790 795 800
Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu
805 810 815
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
820 825 830
Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg
835 840 845
Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
850 855 860
Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu
865 870 875 880
Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr
885 890 895
Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu
900 905 910
Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp
915 920 925
Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val
930 935 940
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp
945 950 955 960
Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His
965 970 975
Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro
980 985 990
Gly Lys
<210> 49
<211> 2982
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 49
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
ggatccgagc caaagtcctc tgataagaca cacacctctc caccatgccc agcaccagag 2340
ctgctgggag gaccaagcgt gttcctgttt cctccaaagc ccaaggacac actgatgatc 2400
tccaggacac cagaggtgac ctgcgtggtg gtggacgtga gccacgagga ccccgaggtg 2460
aagttcaact ggtacgtgga tggcgtggag gtgcacaatg ccaagaccaa gcccagagag 2520
gagcagtaca actctaccta tagggtggtg agcgtgctga cagtgctgca ccaggactgg 2580
ctgaacggca aggagtataa gtgcaaggtg agcaataagg ccctgcctgc cccaatcgag 2640
aagacaatct ccaaggccaa gggccagcca agagagcccc aggtgtacac cctgccccct 2700
agcagggatg agctgacaaa gaaccaggtg tccctgacct gtctggtgaa gggcttttat 2760
ccctccgaca tcgccgtgga gtgggagtct aatggccagc ctgagaataa ctacaagaca 2820
accccacccg tgctggattc tgacggcagc ttctttctgt attctaagct gaccgtggac 2880
aagagcaggt ggcagcaggg caacgtgttc agctgctccg tgatgcacga agcactgcac 2940
aatcactaca cccagaaatc actgtcactg agccctggca aa 2982
<210> 50
<211> 2985
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 50
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
ggatccgagc ctcggggccc taccatcaag ccctgccccc cttgcaagtg ccctgcccct 2340
aatctgctgg gcggaccctc cgtgttcctg tttcctccaa agcccaagga cacactgatg 2400
atctccagga caccagaggt gacctgcgtg gtggtggacg tgagccacga ggaccccgag 2460
gtgaagttca actggtacgt ggatggcgtg gaggtgcaca atgccaagac caagcccaga 2520
gaggagcagt acaactctac ctatagggtg gtgagcgtgc tgacagtgct gcaccaggac 2580
tggctgaacg gcaaggagta taagtgcaag gtgagcaata aggccctgcc tgccccaatc 2640
gagaagacaa tctccaaggc caagggccag ccaagagagc cccaggtgta caccctgccc 2700
cctagcaggg atgagctgac aaagaaccag gtgtccctga cctgtctggt gaagggcttt 2760
tatccctccg acatcgccgt ggagtgggag tctaatggcc agcctgagaa taactacaag 2820
acaaccccac ccgtgctgga ttctgacggc agcttctttc tgtattctaa gctgaccgtg 2880
gacaagagca ggtggcagca gggcaacgtg ttcagctgct ccgtgatgca cgaagcactg 2940
cacaatcact acacccagaa atcactgtca ctgagccctg gcaaa 2985
<210> 51
<211> 3021
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 51
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
ggatcccgca acaccggccg cggcggcgag gagaagaaga aggagaagga gaaggaggag 2340
caggaggagc gcgagaccaa gacccccgag tgccccagcc acacccagcc cctgggcgtg 2400
ttcctgtttc ctccaaagcc caaggacaca ctgatgatct ccaggacacc agaggtgacc 2460
tgcgtggtgg tggacgtgag ccacgaggac cccgaggtga agttcaactg gtacgtggat 2520
ggcgtggagg tgcacaatgc caagaccaag cccagagagg agcagtacaa ctctacctat 2580
agggtggtga gcgtgctgac agtgctgcac caggactggc tgaacggcaa ggagtataag 2640
tgcaaggtga gcaataaggc cctgcctgcc ccaatcgaga agacaatctc caaggccaag 2700
ggccagccaa gagagcccca ggtgtacacc ctgcccccta gcagggatga gctgacaaag 2760
aaccaggtgt ccctgacctg tctggtgaag ggcttttatc cctccgacat cgccgtggag 2820
tgggagtcta atggccagcc tgagaataac tacaagacaa ccccacccgt gctggattct 2880
gacggcagct tctttctgta ttctaagctg accgtggaca agagcaggtg gcagcagggc 2940
aacgtgttca gctgctccgt gatgcacgaa gcactgcaca atcactacac ccagaaatca 3000
ctgtcactga gccctggcaa a 3021
<210> 52
<211> 2982
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 52
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
ggatccgaac cgaaatcttc tgacaaaacc cacacctctc cgccgtctcc ggctccggaa 2340
ctgctgggtg gttcttctgt tttcctgttt cctccaaagc ccaaggacac actgatgatc 2400
tccaggacac cagaggtgac ctgcgtggtg gtggacgtga gccacgagga ccccgaggtg 2460
aagttcaact ggtacgtgga tggcgtggag gtgcacaatg ccaagaccaa gcccagagag 2520
gagcagtaca actctaccta tagggtggtg agcgtgctga cagtgctgca ccaggactgg 2580
ctgaacggca aggagtataa gtgcaaggtg agcaataagg ccctgcctgc cccaatcgag 2640
aagacaatct ccaaggccaa gggccagcca agagagcccc aggtgtacac cctgccccct 2700
agcagggatg agctgacaaa gaaccaggtg tccctgacct gtctggtgaa gggcttttat 2760
ccctccgaca tcgccgtgga gtgggagtct aatggccagc ctgagaataa ctacaagaca 2820
accccacccg tgctggattc tgacggcagc ttctttctgt attctaagct gaccgtggac 2880
aagagcaggt ggcagcaggg caacgtgttc agctgctccg tgatgcacga agcactgcac 2940
aatcactaca cccagaaatc actgtcactg agccctggca aa 2982
<210> 53
<211> 994
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 53
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Gly Ser Glu Pro Lys Ser Ser Asp
755 760 765
Lys Thr His Thr Ser Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly
770 775 780
Pro Ser Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile
785 790 795 800
Ser Leu Ser Pro Ile Val Thr Cys Val Val Val Asp Val Ser Glu Asp
805 810 815
Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His
820 825 830
Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu Arg
835 840 845
Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys
850 855 860
Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu
865 870 875 880
Arg Thr Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val Tyr
885 890 895
Val Leu Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr Leu
900 905 910
Thr Cys Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu Trp
915 920 925
Thr Asn Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val
930 935 940
Leu Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu
945 950 955 960
Lys Lys Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val His
965 970 975
Glu Gly Leu His Asn His His Thr Thr Lys Ser Phe Ser Arg Thr Pro
980 985 990
Gly Lys
<210> 54
<211> 995
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 54
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Gly Ser Glu Pro Arg Gly Pro Thr
755 760 765
Ile Lys Pro Cys Pro Pro Cys Lys Cys Pro Ala Pro Asn Leu Leu Gly
770 775 780
Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met
785 790 795 800
Ile Ser Leu Ser Pro Ile Val Thr Cys Val Val Val Asp Val Ser Glu
805 810 815
Asp Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val
820 825 830
His Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu
835 840 845
Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly
850 855 860
Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile
865 870 875 880
Glu Arg Thr Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val
885 890 895
Tyr Val Leu Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr
900 905 910
Leu Thr Cys Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu
915 920 925
Trp Thr Asn Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro
930 935 940
Val Leu Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val
945 950 955 960
Glu Lys Lys Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val
965 970 975
His Glu Gly Leu His Asn His His Thr Thr Lys Ser Phe Ser Arg Thr
980 985 990
Pro Gly Lys
995
<210> 55
<211> 1007
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 55
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Gly Ser Arg Asn Thr Gly Arg Gly
755 760 765
Gly Glu Glu Lys Lys Lys Glu Lys Glu Lys Glu Glu Gln Glu Glu Arg
770 775 780
Glu Thr Lys Thr Pro Glu Cys Pro Ser His Thr Gln Pro Leu Gly Val
785 790 795 800
Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile Ser Leu Ser
805 810 815
Pro Ile Val Thr Cys Val Val Val Asp Val Ser Glu Asp Asp Pro Asp
820 825 830
Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His Thr Ala Gln
835 840 845
Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu Arg Val Val Ser
850 855 860
Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys Glu Phe Lys
865 870 875 880
Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu Arg Thr Ile
885 890 895
Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val Tyr Val Leu Pro
900 905 910
Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr Leu Thr Cys Met
915 920 925
Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu Trp Thr Asn Asn
930 935 940
Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val Leu Asp Ser
945 950 955 960
Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu Lys Lys Asn
965 970 975
Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val His Glu Gly Leu
980 985 990
His Asn His His Thr Thr Lys Ser Phe Ser Arg Thr Pro Gly Lys
995 1000 1005
<210> 56
<211> 994
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 56
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Gly Ser Glu Pro Lys Ser Ser Asp
755 760 765
Lys Thr His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly
770 775 780
Ser Ser Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile
785 790 795 800
Ser Leu Ser Pro Ile Val Thr Cys Val Val Val Asp Val Ser Glu Asp
805 810 815
Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His
820 825 830
Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu Arg
835 840 845
Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys
850 855 860
Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu
865 870 875 880
Arg Thr Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val Tyr
885 890 895
Val Leu Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr Leu
900 905 910
Thr Cys Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu Trp
915 920 925
Thr Asn Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val
930 935 940
Leu Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu
945 950 955 960
Lys Lys Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val His
965 970 975
Glu Gly Leu His Asn His His Thr Thr Lys Ser Phe Ser Arg Thr Pro
980 985 990
Gly Lys
<210> 57
<211> 2982
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 57
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
ggatccgagc caaagtcctc tgataagaca cacacctctc caccatgccc agcaccagag 2340
ctgctgggag gaccaagcgt gttcatcttc ccacccaaga tcaaggacgt gctgatgatc 2400
tccctgtccc ccatcgtgac ctgcgtggtg gtggacgtgt ccgaggacga ccccgacgtg 2460
cagatcagtt ggttcgtgaa caacgtggaa gtgcacaccg cccagaccca gacccacaga 2520
gaggactaca actccaccct gcgggtggtg tccgccctgc ccatccagca ccaggactgg 2580
atgtccggca aagaattcaa gtgcaaagtg aacaacaagg acctgcctgc ccccatcgag 2640
cggaccatct ccaagcccaa gggctccgtg cgggctcccc aggtgtacgt gctgccccct 2700
ccagaggaag agatgaccaa gaagcaggtc acactgacct gcatggtcac cgacttcatg 2760
cccgaggaca tctacgtgga atggaccaac aatggcaaga ccgagctgaa ctacaagaac 2820
accgagcctg tgctggactc cgacggctcc tacttcatgt actccaagct gcgggtggaa 2880
aagaagaact gggtcgagcg gaactcctac tcctgctccg tggtgcacga gggcctgcac 2940
aaccaccaca ccaccaagtc cttctcccgg acccccggca aa 2982
<210> 58
<211> 2985
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 58
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
ggatccgagc ctcggggccc taccatcaag ccctgccccc cttgcaagtg ccctgcccct 2340
aatctgctgg gcggaccctc cgtgttcatc ttcccaccca agatcaagga cgtgctgatg 2400
atctccctgt cccccatcgt gacctgcgtg gtggtggacg tgtccgagga cgaccccgac 2460
gtgcagatca gttggttcgt gaacaacgtg gaagtgcaca ccgcccagac ccagacccac 2520
agagaggact acaactccac cctgcgggtg gtgtccgccc tgcccatcca gcaccaggac 2580
tggatgtccg gcaaagaatt caagtgcaaa gtgaacaaca aggacctgcc tgcccccatc 2640
gagcggacca tctccaagcc caagggctcc gtgcgggctc cccaggtgta cgtgctgccc 2700
cctccagagg aagagatgac caagaagcag gtcacactga cctgcatggt caccgacttc 2760
atgcccgagg acatctacgt ggaatggacc aacaatggca agaccgagct gaactacaag 2820
aacaccgagc ctgtgctgga ctccgacggc tcctacttca tgtactccaa gctgcgggtg 2880
gaaaagaaga actgggtcga gcggaactcc tactcctgct ccgtggtgca cgagggcctg 2940
cacaaccacc acaccaccaa gtccttctcc cggacccccg gcaaa 2985
<210> 59
<211> 3021
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 59
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
ggatcccgca acaccggccg cggcggcgag gagaagaaga aggagaagga gaaggaggag 2340
caggaggagc gcgagaccaa gacccccgag tgccccagcc acacccagcc cctgggcgtg 2400
ttcatcttcc cacccaagat caaggacgtg ctgatgatct ccctgtcccc catcgtgacc 2460
tgcgtggtgg tggacgtgtc cgaggacgac cccgacgtgc agatcagttg gttcgtgaac 2520
aacgtggaag tgcacaccgc ccagacccag acccacagag aggactacaa ctccaccctg 2580
cgggtggtgt ccgccctgcc catccagcac caggactgga tgtccggcaa agaattcaag 2640
tgcaaagtga acaacaagga cctgcctgcc cccatcgagc ggaccatctc caagcccaag 2700
ggctccgtgc gggctcccca ggtgtacgtg ctgccccctc cagaggaaga gatgaccaag 2760
aagcaggtca cactgacctg catggtcacc gacttcatgc ccgaggacat ctacgtggaa 2820
tggaccaaca atggcaagac cgagctgaac tacaagaaca ccgagcctgt gctggactcc 2880
gacggctcct acttcatgta ctccaagctg cgggtggaaa agaagaactg ggtcgagcgg 2940
aactcctact cctgctccgt ggtgcacgag ggcctgcaca accaccacac caccaagtcc 3000
ttctcccgga cccccggcaa a 3021
<210> 60
<211> 2982
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 60
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
ggatccgaac cgaaatcttc tgacaaaacc cacacctctc cgccgtctcc ggctccggaa 2340
ctgctgggtg gttcttctgt tttcatcttc ccacccaaga tcaaggacgt gctgatgatc 2400
tccctgtccc ccatcgtgac ctgcgtggtg gtggacgtgt ccgaggacga ccccgacgtg 2460
cagatcagtt ggttcgtgaa caacgtggaa gtgcacaccg cccagaccca gacccacaga 2520
gaggactaca actccaccct gcgggtggtg tccgccctgc ccatccagca ccaggactgg 2580
atgtccggca aagaattcaa gtgcaaagtg aacaacaagg acctgcctgc ccccatcgag 2640
cggaccatct ccaagcccaa gggctccgtg cgggctcccc aggtgtacgt gctgccccct 2700
ccagaggaag agatgaccaa gaagcaggtc acactgacct gcatggtcac cgacttcatg 2760
cccgaggaca tctacgtgga atggaccaac aatggcaaga ccgagctgaa ctacaagaac 2820
accgagcctg tgctggactc cgacggctcc tacttcatgt actccaagct gcgggtggaa 2880
aagaagaact gggtcgagcg gaactcctac tcctgctccg tggtgcacga gggcctgcac 2940
aaccaccaca ccaccaagtc cttctcccgg acccccggca aa 2982
<210> 61
<211> 999
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 61
Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala Gly Asp
1 5 10 15
Ser Leu Asp Cys Gly Gly Arg Gly Leu Ala Ala Leu Pro Gly Asp Leu
20 25 30
Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Lys Leu Ser Glu
35 40 45
Ile Asp Pro Ala Gly Phe Glu Asp Leu Pro Asn Leu Gln Glu Val Tyr
50 55 60
Leu Asn Asn Asn Glu Leu Thr Ala Val Pro Ser Leu Gly Ala Ala Ser
65 70 75 80
Ser His Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Arg Ser Val
85 90 95
Glu Gly Ser Gln Leu Lys Ala Tyr Leu Ser Leu Glu Val Leu Asp Leu
100 105 110
Ser Leu Asn Asn Ile Thr Glu Val Arg Asn Thr Cys Phe Pro His Gly
115 120 125
Pro Pro Ile Lys Glu Leu Asn Leu Ala Gly Asn Arg Ile Gly Thr Leu
130 135 140
Glu Leu Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr Leu Arg
145 150 155 160
Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Arg Ala Phe Lys Leu
165 170 175
Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg Leu Ile
180 185 190
Glu Gly Leu Thr Phe Gln Gly Leu Asn Ser Leu Glu Val Leu Lys Leu
195 200 205
Gln Arg Asn Asn Ile Ser Lys Leu Thr Asp Gly Ala Phe Trp Gly Leu
210 215 220
Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val Glu Val
225 230 235 240
Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu His Leu
245 250 255
Ser Asn Asn Ser Ile Ala Arg Ile His Arg Lys Gly Trp Ser Phe Cys
260 265 270
Gln Lys Leu His Glu Leu Val Leu Ser Phe Asn Asn Leu Thr Arg Leu
275 280 285
Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Val Leu Arg Leu
290 295 300
Ser His Asn Ser Ile Ser His Ile Ala Glu Gly Ala Phe Lys Gly Leu
305 310 315 320
Arg Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser Gly Thr
325 330 335
Ile Glu Asp Thr Ser Gly Ala Phe Ser Gly Leu Asp Ser Leu Ser Lys
340 345 350
Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg Ala Phe
355 360 365
Ser Gly Leu Glu Gly Leu Glu His Leu Asn Leu Gly Gly Asn Ala Ile
370 375 380
Arg Ser Val Gln Phe Asp Ala Phe Val Lys Met Lys Asn Leu Lys Glu
385 390 395 400
Leu His Ile Ser Ser Asp Ser Phe Leu Cys Asp Cys Gln Leu Lys Trp
405 410 415
Leu Pro Pro Trp Leu Ile Gly Arg Met Leu Gln Ala Phe Val Thr Ala
420 425 430
Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe Ser Val
435 440 445
Pro Pro Glu Ser Phe Val Cys Asp Asp Phe Leu Lys Pro Gln Ile Ile
450 455 460
Thr Gln Pro Glu Thr Thr Met Ala Met Val Gly Lys Asp Ile Arg Phe
465 470 475 480
Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe Ala Trp
485 490 495
Lys Lys Asp Asn Glu Val Leu Thr Asn Ala Asp Met Glu Asn Phe Val
500 505 510
His Val His Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr Ile Leu
515 520 525
His Leu Arg Gln Val Thr Phe Gly His Glu Gly Arg Tyr Gln Cys Val
530 535 540
Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg Leu Thr
545 550 555 560
Val Asn Val Leu Pro Ser Phe Thr Lys Thr Pro His Asp Ile Thr Ile
565 570 575
Arg Thr Thr Thr Val Ala Arg Leu Glu Cys Ala Ala Thr Gly His Pro
580 585 590
Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe Pro Ala
595 600 605
Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val Phe Phe
610 615 620
Ile Thr Asp Val Lys Ile Asp Asp Ala Gly Val Tyr Ser Cys Thr Ala
625 630 635 640
Gln Asn Ser Ala Gly Ser Ile Ser Ala Asn Ala Thr Leu Thr Val Leu
645 650 655
Glu Thr Pro Ser Leu Val Val Pro Leu Glu Asp Arg Val Val Ser Val
660 665 670
Gly Glu Thr Val Ala Leu Gln Cys Lys Ala Thr Gly Asn Pro Pro Pro
675 680 685
Arg Ile Thr Trp Phe Lys Gly Asp Arg Pro Leu Ser Leu Thr Glu Arg
690 695 700
His His Leu Thr Pro Asp Asn Gln Leu Leu Val Val Gln Asn Val Val
705 710 715 720
Ala Glu Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Thr Leu Gly
725 730 735
Thr Glu Arg Ala His Ser Gln Leu Ser Val Leu Pro Ala Ala Gly Cys
740 745 750
Arg Lys Asp Gly Thr Thr Val Gly Ile Phe Gly Gly Gly Gly Ser Glu
755 760 765
Pro Lys Ser Ser Asp Lys Thr His Thr Ser Pro Pro Cys Pro Ala Pro
770 775 780
Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys
785 790 795 800
Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val
805 810 815
Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp
820 825 830
Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr
835 840 845
Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp
850 855 860
Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
865 870 875 880
Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg
885 890 895
Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys
900 905 910
Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp
915 920 925
Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys
930 935 940
Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser
945 950 955 960
Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser
965 970 975
Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser
980 985 990
Leu Ser Leu Ser Pro Gly Lys
995
<210> 62
<211> 1000
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 62
Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala Gly Asp
1 5 10 15
Ser Leu Asp Cys Gly Gly Arg Gly Leu Ala Ala Leu Pro Gly Asp Leu
20 25 30
Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Lys Leu Ser Glu
35 40 45
Ile Asp Pro Ala Gly Phe Glu Asp Leu Pro Asn Leu Gln Glu Val Tyr
50 55 60
Leu Asn Asn Asn Glu Leu Thr Ala Val Pro Ser Leu Gly Ala Ala Ser
65 70 75 80
Ser His Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Arg Ser Val
85 90 95
Glu Gly Ser Gln Leu Lys Ala Tyr Leu Ser Leu Glu Val Leu Asp Leu
100 105 110
Ser Leu Asn Asn Ile Thr Glu Val Arg Asn Thr Cys Phe Pro His Gly
115 120 125
Pro Pro Ile Lys Glu Leu Asn Leu Ala Gly Asn Arg Ile Gly Thr Leu
130 135 140
Glu Leu Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr Leu Arg
145 150 155 160
Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Arg Ala Phe Lys Leu
165 170 175
Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg Leu Ile
180 185 190
Glu Gly Leu Thr Phe Gln Gly Leu Asn Ser Leu Glu Val Leu Lys Leu
195 200 205
Gln Arg Asn Asn Ile Ser Lys Leu Thr Asp Gly Ala Phe Trp Gly Leu
210 215 220
Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val Glu Val
225 230 235 240
Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu His Leu
245 250 255
Ser Asn Asn Ser Ile Ala Arg Ile His Arg Lys Gly Trp Ser Phe Cys
260 265 270
Gln Lys Leu His Glu Leu Val Leu Ser Phe Asn Asn Leu Thr Arg Leu
275 280 285
Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Val Leu Arg Leu
290 295 300
Ser His Asn Ser Ile Ser His Ile Ala Glu Gly Ala Phe Lys Gly Leu
305 310 315 320
Arg Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser Gly Thr
325 330 335
Ile Glu Asp Thr Ser Gly Ala Phe Ser Gly Leu Asp Ser Leu Ser Lys
340 345 350
Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg Ala Phe
355 360 365
Ser Gly Leu Glu Gly Leu Glu His Leu Asn Leu Gly Gly Asn Ala Ile
370 375 380
Arg Ser Val Gln Phe Asp Ala Phe Val Lys Met Lys Asn Leu Lys Glu
385 390 395 400
Leu His Ile Ser Ser Asp Ser Phe Leu Cys Asp Cys Gln Leu Lys Trp
405 410 415
Leu Pro Pro Trp Leu Ile Gly Arg Met Leu Gln Ala Phe Val Thr Ala
420 425 430
Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe Ser Val
435 440 445
Pro Pro Glu Ser Phe Val Cys Asp Asp Phe Leu Lys Pro Gln Ile Ile
450 455 460
Thr Gln Pro Glu Thr Thr Met Ala Met Val Gly Lys Asp Ile Arg Phe
465 470 475 480
Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe Ala Trp
485 490 495
Lys Lys Asp Asn Glu Val Leu Thr Asn Ala Asp Met Glu Asn Phe Val
500 505 510
His Val His Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr Ile Leu
515 520 525
His Leu Arg Gln Val Thr Phe Gly His Glu Gly Arg Tyr Gln Cys Val
530 535 540
Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg Leu Thr
545 550 555 560
Val Asn Val Leu Pro Ser Phe Thr Lys Thr Pro His Asp Ile Thr Ile
565 570 575
Arg Thr Thr Thr Val Ala Arg Leu Glu Cys Ala Ala Thr Gly His Pro
580 585 590
Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe Pro Ala
595 600 605
Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val Phe Phe
610 615 620
Ile Thr Asp Val Lys Ile Asp Asp Ala Gly Val Tyr Ser Cys Thr Ala
625 630 635 640
Gln Asn Ser Ala Gly Ser Ile Ser Ala Asn Ala Thr Leu Thr Val Leu
645 650 655
Glu Thr Pro Ser Leu Val Val Pro Leu Glu Asp Arg Val Val Ser Val
660 665 670
Gly Glu Thr Val Ala Leu Gln Cys Lys Ala Thr Gly Asn Pro Pro Pro
675 680 685
Arg Ile Thr Trp Phe Lys Gly Asp Arg Pro Leu Ser Leu Thr Glu Arg
690 695 700
His His Leu Thr Pro Asp Asn Gln Leu Leu Val Val Gln Asn Val Val
705 710 715 720
Ala Glu Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Thr Leu Gly
725 730 735
Thr Glu Arg Ala His Ser Gln Leu Ser Val Leu Pro Ala Ala Gly Cys
740 745 750
Arg Lys Asp Gly Thr Thr Val Gly Ile Phe Gly Gly Gly Gly Ser Glu
755 760 765
Pro Arg Gly Pro Thr Ile Lys Pro Cys Pro Pro Cys Lys Cys Pro Ala
770 775 780
Pro Asn Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro
785 790 795 800
Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val
805 810 815
Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val
820 825 830
Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln
835 840 845
Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln
850 855 860
Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala
865 870 875 880
Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro
885 890 895
Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr
900 905 910
Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser
915 920 925
Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr
930 935 940
Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr
945 950 955 960
Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe
965 970 975
Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys
980 985 990
Ser Leu Ser Leu Ser Pro Gly Lys
995 1000
<210> 63
<211> 1012
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 63
Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala Gly Asp
1 5 10 15
Ser Leu Asp Cys Gly Gly Arg Gly Leu Ala Ala Leu Pro Gly Asp Leu
20 25 30
Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Lys Leu Ser Glu
35 40 45
Ile Asp Pro Ala Gly Phe Glu Asp Leu Pro Asn Leu Gln Glu Val Tyr
50 55 60
Leu Asn Asn Asn Glu Leu Thr Ala Val Pro Ser Leu Gly Ala Ala Ser
65 70 75 80
Ser His Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Arg Ser Val
85 90 95
Glu Gly Ser Gln Leu Lys Ala Tyr Leu Ser Leu Glu Val Leu Asp Leu
100 105 110
Ser Leu Asn Asn Ile Thr Glu Val Arg Asn Thr Cys Phe Pro His Gly
115 120 125
Pro Pro Ile Lys Glu Leu Asn Leu Ala Gly Asn Arg Ile Gly Thr Leu
130 135 140
Glu Leu Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr Leu Arg
145 150 155 160
Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Arg Ala Phe Lys Leu
165 170 175
Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg Leu Ile
180 185 190
Glu Gly Leu Thr Phe Gln Gly Leu Asn Ser Leu Glu Val Leu Lys Leu
195 200 205
Gln Arg Asn Asn Ile Ser Lys Leu Thr Asp Gly Ala Phe Trp Gly Leu
210 215 220
Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val Glu Val
225 230 235 240
Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu His Leu
245 250 255
Ser Asn Asn Ser Ile Ala Arg Ile His Arg Lys Gly Trp Ser Phe Cys
260 265 270
Gln Lys Leu His Glu Leu Val Leu Ser Phe Asn Asn Leu Thr Arg Leu
275 280 285
Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Val Leu Arg Leu
290 295 300
Ser His Asn Ser Ile Ser His Ile Ala Glu Gly Ala Phe Lys Gly Leu
305 310 315 320
Arg Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser Gly Thr
325 330 335
Ile Glu Asp Thr Ser Gly Ala Phe Ser Gly Leu Asp Ser Leu Ser Lys
340 345 350
Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg Ala Phe
355 360 365
Ser Gly Leu Glu Gly Leu Glu His Leu Asn Leu Gly Gly Asn Ala Ile
370 375 380
Arg Ser Val Gln Phe Asp Ala Phe Val Lys Met Lys Asn Leu Lys Glu
385 390 395 400
Leu His Ile Ser Ser Asp Ser Phe Leu Cys Asp Cys Gln Leu Lys Trp
405 410 415
Leu Pro Pro Trp Leu Ile Gly Arg Met Leu Gln Ala Phe Val Thr Ala
420 425 430
Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe Ser Val
435 440 445
Pro Pro Glu Ser Phe Val Cys Asp Asp Phe Leu Lys Pro Gln Ile Ile
450 455 460
Thr Gln Pro Glu Thr Thr Met Ala Met Val Gly Lys Asp Ile Arg Phe
465 470 475 480
Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe Ala Trp
485 490 495
Lys Lys Asp Asn Glu Val Leu Thr Asn Ala Asp Met Glu Asn Phe Val
500 505 510
His Val His Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr Ile Leu
515 520 525
His Leu Arg Gln Val Thr Phe Gly His Glu Gly Arg Tyr Gln Cys Val
530 535 540
Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg Leu Thr
545 550 555 560
Val Asn Val Leu Pro Ser Phe Thr Lys Thr Pro His Asp Ile Thr Ile
565 570 575
Arg Thr Thr Thr Val Ala Arg Leu Glu Cys Ala Ala Thr Gly His Pro
580 585 590
Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe Pro Ala
595 600 605
Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val Phe Phe
610 615 620
Ile Thr Asp Val Lys Ile Asp Asp Ala Gly Val Tyr Ser Cys Thr Ala
625 630 635 640
Gln Asn Ser Ala Gly Ser Ile Ser Ala Asn Ala Thr Leu Thr Val Leu
645 650 655
Glu Thr Pro Ser Leu Val Val Pro Leu Glu Asp Arg Val Val Ser Val
660 665 670
Gly Glu Thr Val Ala Leu Gln Cys Lys Ala Thr Gly Asn Pro Pro Pro
675 680 685
Arg Ile Thr Trp Phe Lys Gly Asp Arg Pro Leu Ser Leu Thr Glu Arg
690 695 700
His His Leu Thr Pro Asp Asn Gln Leu Leu Val Val Gln Asn Val Val
705 710 715 720
Ala Glu Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Thr Leu Gly
725 730 735
Thr Glu Arg Ala His Ser Gln Leu Ser Val Leu Pro Ala Ala Gly Cys
740 745 750
Arg Lys Asp Gly Thr Thr Val Gly Ile Phe Gly Gly Gly Gly Ser Arg
755 760 765
Asn Thr Gly Arg Gly Gly Glu Glu Lys Lys Lys Glu Lys Glu Lys Glu
770 775 780
Glu Gln Glu Glu Arg Glu Thr Lys Thr Pro Glu Cys Pro Ser His Thr
785 790 795 800
Gln Pro Leu Gly Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu
805 810 815
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser
820 825 830
His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu
835 840 845
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr
850 855 860
Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn
865 870 875 880
Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
885 890 895
Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln
900 905 910
Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val
915 920 925
Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val
930 935 940
Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro
945 950 955 960
Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr
965 970 975
Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val
980 985 990
Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu
995 1000 1005
Ser Pro Gly Lys
1010
<210> 64
<211> 999
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 64
Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala Gly Asp
1 5 10 15
Ser Leu Asp Cys Gly Gly Arg Gly Leu Ala Ala Leu Pro Gly Asp Leu
20 25 30
Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Lys Leu Ser Glu
35 40 45
Ile Asp Pro Ala Gly Phe Glu Asp Leu Pro Asn Leu Gln Glu Val Tyr
50 55 60
Leu Asn Asn Asn Glu Leu Thr Ala Val Pro Ser Leu Gly Ala Ala Ser
65 70 75 80
Ser His Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Arg Ser Val
85 90 95
Glu Gly Ser Gln Leu Lys Ala Tyr Leu Ser Leu Glu Val Leu Asp Leu
100 105 110
Ser Leu Asn Asn Ile Thr Glu Val Arg Asn Thr Cys Phe Pro His Gly
115 120 125
Pro Pro Ile Lys Glu Leu Asn Leu Ala Gly Asn Arg Ile Gly Thr Leu
130 135 140
Glu Leu Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr Leu Arg
145 150 155 160
Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Arg Ala Phe Lys Leu
165 170 175
Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg Leu Ile
180 185 190
Glu Gly Leu Thr Phe Gln Gly Leu Asn Ser Leu Glu Val Leu Lys Leu
195 200 205
Gln Arg Asn Asn Ile Ser Lys Leu Thr Asp Gly Ala Phe Trp Gly Leu
210 215 220
Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val Glu Val
225 230 235 240
Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu His Leu
245 250 255
Ser Asn Asn Ser Ile Ala Arg Ile His Arg Lys Gly Trp Ser Phe Cys
260 265 270
Gln Lys Leu His Glu Leu Val Leu Ser Phe Asn Asn Leu Thr Arg Leu
275 280 285
Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Val Leu Arg Leu
290 295 300
Ser His Asn Ser Ile Ser His Ile Ala Glu Gly Ala Phe Lys Gly Leu
305 310 315 320
Arg Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser Gly Thr
325 330 335
Ile Glu Asp Thr Ser Gly Ala Phe Ser Gly Leu Asp Ser Leu Ser Lys
340 345 350
Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg Ala Phe
355 360 365
Ser Gly Leu Glu Gly Leu Glu His Leu Asn Leu Gly Gly Asn Ala Ile
370 375 380
Arg Ser Val Gln Phe Asp Ala Phe Val Lys Met Lys Asn Leu Lys Glu
385 390 395 400
Leu His Ile Ser Ser Asp Ser Phe Leu Cys Asp Cys Gln Leu Lys Trp
405 410 415
Leu Pro Pro Trp Leu Ile Gly Arg Met Leu Gln Ala Phe Val Thr Ala
420 425 430
Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe Ser Val
435 440 445
Pro Pro Glu Ser Phe Val Cys Asp Asp Phe Leu Lys Pro Gln Ile Ile
450 455 460
Thr Gln Pro Glu Thr Thr Met Ala Met Val Gly Lys Asp Ile Arg Phe
465 470 475 480
Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe Ala Trp
485 490 495
Lys Lys Asp Asn Glu Val Leu Thr Asn Ala Asp Met Glu Asn Phe Val
500 505 510
His Val His Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr Ile Leu
515 520 525
His Leu Arg Gln Val Thr Phe Gly His Glu Gly Arg Tyr Gln Cys Val
530 535 540
Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg Leu Thr
545 550 555 560
Val Asn Val Leu Pro Ser Phe Thr Lys Thr Pro His Asp Ile Thr Ile
565 570 575
Arg Thr Thr Thr Val Ala Arg Leu Glu Cys Ala Ala Thr Gly His Pro
580 585 590
Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe Pro Ala
595 600 605
Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val Phe Phe
610 615 620
Ile Thr Asp Val Lys Ile Asp Asp Ala Gly Val Tyr Ser Cys Thr Ala
625 630 635 640
Gln Asn Ser Ala Gly Ser Ile Ser Ala Asn Ala Thr Leu Thr Val Leu
645 650 655
Glu Thr Pro Ser Leu Val Val Pro Leu Glu Asp Arg Val Val Ser Val
660 665 670
Gly Glu Thr Val Ala Leu Gln Cys Lys Ala Thr Gly Asn Pro Pro Pro
675 680 685
Arg Ile Thr Trp Phe Lys Gly Asp Arg Pro Leu Ser Leu Thr Glu Arg
690 695 700
His His Leu Thr Pro Asp Asn Gln Leu Leu Val Val Gln Asn Val Val
705 710 715 720
Ala Glu Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Thr Leu Gly
725 730 735
Thr Glu Arg Ala His Ser Gln Leu Ser Val Leu Pro Ala Ala Gly Cys
740 745 750
Arg Lys Asp Gly Thr Thr Val Gly Ile Phe Gly Gly Gly Gly Ser Glu
755 760 765
Pro Lys Ser Ser Asp Lys Thr His Thr Ser Pro Pro Ser Pro Ala Pro
770 775 780
Glu Leu Leu Gly Gly Ser Ser Val Phe Leu Phe Pro Pro Lys Pro Lys
785 790 795 800
Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val
805 810 815
Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp
820 825 830
Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr
835 840 845
Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp
850 855 860
Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu
865 870 875 880
Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg
885 890 895
Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys
900 905 910
Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp
915 920 925
Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys
930 935 940
Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser
945 950 955 960
Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser
965 970 975
Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser
980 985 990
Leu Ser Leu Ser Pro Gly Lys
995
<210> 65
<211> 2997
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 65
gctgggcctc gggctccttg tgctgccgcc tgcacatgtg caggcgattc cctggactgc 60
ggcggcagag gcctggccgc cctgcctggc gatctgccat cctggacccg gagcctgaac 120
ctgagctaca acaagctgag cgagatcgat cccgccggct ttgaggacct gcctaacctg 180
caggaggtgt atctgaacaa taacgagctg accgcggtac catccctggg cgctgcttca 240
tcacatgtcg tctctctctt tctgcagcac aacaagattc gcagcgtgga ggggagccag 300
ctgaaggcct acctttcctt agaagtgtta gatctgagtt tgaacaacat cacggaagtg 360
cggaacacct gctttccaca cggaccgcct ataaaggagc tcaacctggc aggcaatcgg 420
attggcaccc tggagttggg agcatttgat ggtctgtcac ggtcgctgct aactcttcgc 480
ctgagcaaaa acaggatcac ccagcttcct gtaagagcat tcaagctacc caggctgaca 540
caactggacc tcaatcggaa caggattcgg ctgatagagg gcctcacctt ccaggggctc 600
aacagcttgg aggtgctgaa gcttcagcga aacaacatca gcaaactgac agatggggcc 660
ttctggggac tgtccaagat gcatgtgctg cacctggagt acaacagcct ggtagaagtg 720
aacagcggct cgctctacgg cctcacggcc ctgcatcagc tccacctcag caacaattcc 780
atcgctcgca ttcaccgcaa gggctggagc ttctgccaga agctgcatga gttggtcctg 840
tccttcaaca acctgacacg gctggacgag gagagcctgg ccgagctgag cagcctgagt 900
gtcctgcgtc tcagccacaa ttccatcagc cacattgcgg agggtgcctt caagggactc 960
aggagcctgc gagtcttgga tctggaccat aacgagattt cgggcacaat agaggacacg 1020
agcggcgcct tctcagggct cgacagcctc agcaagctga ctctgtttgg aaacaagatc 1080
aagtctgtgg ctaagagagc attctcgggg ctggaaggcc tggagcacct gaaccttgga 1140
gggaatgcga tcagatctgt ccagtttgat gcctttgtga agatgaagaa tcttaaagag 1200
ctccatatca gcagcgacag cttcctgtgt gactgccagc tgaagtggct gcccccgtgg 1260
ctaattggca ggatgctgca ggcctttgtg acagccacct gtgcccaccc agaatcactg 1320
aagggtcaga gcattttctc tgtgccacca gagagtttcg tgtgcgatga cttcctgaag 1380
ccacagatca tcacccagcc agaaaccacc atggctatgg tgggcaagga catccggttt 1440
acatgctcag cagccagcag cagcagctcc cccatgacct ttgcctggaa gaaagacaat 1500
gaagtcctga ccaatgcaga catggagaac tttgtccacg tccacgcgca ggacggggaa 1560
gtgatggagt acaccaccat cctgcacctc cgtcaggtca ctttcgggca cgagggccgc 1620
taccaatgtg tcatcaccaa ccactttggc tccacctatt cacataaggc caggctcacc 1680
gtgaatgtgt tgccatcatt caccaaaacg ccccacgaca taaccatccg gaccaccacc 1740
gtggcccgcc tcgaatgtgc tgccacaggt cacccaaacc ctcagattgc ctggcagaag 1800
gatggaggca cggatttccc cgctgcccgt gagcgacgca tgcatgtcat gccggatgac 1860
gacgtgtttt tcatcactga tgtgaaaata gatgacgcag gggtttacag ctgtactgct 1920
cagaactcag ccggttctat ttcagctaat gccaccctga ctgtcctaga gaccccatcc 1980
ttggtggtcc ccttggaaga ccgtgtggta tctgtgggag aaacagtggc cctccaatgc 2040
aaagccacgg ggaaccctcc gccccgcatc acctggttca agggggaccg cccgctgagc 2100
ctcactgagc ggcaccacct gacccctgac aaccagctcc tggtggttca gaacgtggtg 2160
gcagaggatg cgggccgata tacctgtgag atgtccaaca ccctgggcac ggagcgagct 2220
cacagccagc tgagcgtcct gcccgcagca ggctgcagga aggatgggac cacggtaggc 2280
atcttcggcg gtggcggatc cgagccaaag tcctctgata agacacacac ctctccacca 2340
tgcccagcac cagagctgct gggaggacca agcgtgttcc tgtttcctcc aaagcccaag 2400
gacacactga tgatctccag gacaccagag gtgacctgcg tggtggtgga cgtgagccac 2460
gaggaccccg aggtgaagtt caactggtac gtggatggcg tggaggtgca caatgccaag 2520
accaagccca gagaggagca gtacaactct acctataggg tggtgagcgt gctgacagtg 2580
ctgcaccagg actggctgaa cggcaaggag tataagtgca aggtgagcaa taaggccctg 2640
cctgccccaa tcgagaagac aatctccaag gccaagggcc agccaagaga gccccaggtg 2700
tacaccctgc cccctagcag ggatgagctg acaaagaacc aggtgtccct gacctgtctg 2760
gtgaagggct tttatccctc cgacatcgcc gtggagtggg agtctaatgg ccagcctgag 2820
aataactaca agacaacccc acccgtgctg gattctgacg gcagcttctt tctgtattct 2880
aagctgaccg tggacaagag caggtggcag cagggcaacg tgttcagctg ctccgtgatg 2940
cacgaagcac tgcacaatca ctacacccag aaatcactgt cactgagccc tggcaaa 2997
<210> 66
<211> 3000
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 66
gctgggcctc gggctccttg tgctgccgcc tgcacatgtg caggcgattc cctggactgc 60
ggcggcagag gcctggccgc cctgcctggc gatctgccat cctggacccg gagcctgaac 120
ctgagctaca acaagctgag cgagatcgat cccgccggct ttgaggacct gcctaacctg 180
caggaggtgt atctgaacaa taacgagctg accgcggtac catccctggg cgctgcttca 240
tcacatgtcg tctctctctt tctgcagcac aacaagattc gcagcgtgga ggggagccag 300
ctgaaggcct acctttcctt agaagtgtta gatctgagtt tgaacaacat cacggaagtg 360
cggaacacct gctttccaca cggaccgcct ataaaggagc tcaacctggc aggcaatcgg 420
attggcaccc tggagttggg agcatttgat ggtctgtcac ggtcgctgct aactcttcgc 480
ctgagcaaaa acaggatcac ccagcttcct gtaagagcat tcaagctacc caggctgaca 540
caactggacc tcaatcggaa caggattcgg ctgatagagg gcctcacctt ccaggggctc 600
aacagcttgg aggtgctgaa gcttcagcga aacaacatca gcaaactgac agatggggcc 660
ttctggggac tgtccaagat gcatgtgctg cacctggagt acaacagcct ggtagaagtg 720
aacagcggct cgctctacgg cctcacggcc ctgcatcagc tccacctcag caacaattcc 780
atcgctcgca ttcaccgcaa gggctggagc ttctgccaga agctgcatga gttggtcctg 840
tccttcaaca acctgacacg gctggacgag gagagcctgg ccgagctgag cagcctgagt 900
gtcctgcgtc tcagccacaa ttccatcagc cacattgcgg agggtgcctt caagggactc 960
aggagcctgc gagtcttgga tctggaccat aacgagattt cgggcacaat agaggacacg 1020
agcggcgcct tctcagggct cgacagcctc agcaagctga ctctgtttgg aaacaagatc 1080
aagtctgtgg ctaagagagc attctcgggg ctggaaggcc tggagcacct gaaccttgga 1140
gggaatgcga tcagatctgt ccagtttgat gcctttgtga agatgaagaa tcttaaagag 1200
ctccatatca gcagcgacag cttcctgtgt gactgccagc tgaagtggct gcccccgtgg 1260
ctaattggca ggatgctgca ggcctttgtg acagccacct gtgcccaccc agaatcactg 1320
aagggtcaga gcattttctc tgtgccacca gagagtttcg tgtgcgatga cttcctgaag 1380
ccacagatca tcacccagcc agaaaccacc atggctatgg tgggcaagga catccggttt 1440
acatgctcag cagccagcag cagcagctcc cccatgacct ttgcctggaa gaaagacaat 1500
gaagtcctga ccaatgcaga catggagaac tttgtccacg tccacgcgca ggacggggaa 1560
gtgatggagt acaccaccat cctgcacctc cgtcaggtca ctttcgggca cgagggccgc 1620
taccaatgtg tcatcaccaa ccactttggc tccacctatt cacataaggc caggctcacc 1680
gtgaatgtgt tgccatcatt caccaaaacg ccccacgaca taaccatccg gaccaccacc 1740
gtggcccgcc tcgaatgtgc tgccacaggt cacccaaacc ctcagattgc ctggcagaag 1800
gatggaggca cggatttccc cgctgcccgt gagcgacgca tgcatgtcat gccggatgac 1860
gacgtgtttt tcatcactga tgtgaaaata gatgacgcag gggtttacag ctgtactgct 1920
cagaactcag ccggttctat ttcagctaat gccaccctga ctgtcctaga gaccccatcc 1980
ttggtggtcc ccttggaaga ccgtgtggta tctgtgggag aaacagtggc cctccaatgc 2040
aaagccacgg ggaaccctcc gccccgcatc acctggttca agggggaccg cccgctgagc 2100
ctcactgagc ggcaccacct gacccctgac aaccagctcc tggtggttca gaacgtggtg 2160
gcagaggatg cgggccgata tacctgtgag atgtccaaca ccctgggcac ggagcgagct 2220
cacagccagc tgagcgtcct gcccgcagca ggctgcagga aggatgggac cacggtaggc 2280
atcttcggcg gtggcggatc cgagcctcgg ggccctacca tcaagccctg ccccccttgc 2340
aagtgccctg cccctaatct gctgggcgga ccctccgtgt tcctgtttcc tccaaagccc 2400
aaggacacac tgatgatctc caggacacca gaggtgacct gcgtggtggt ggacgtgagc 2460
cacgaggacc ccgaggtgaa gttcaactgg tacgtggatg gcgtggaggt gcacaatgcc 2520
aagaccaagc ccagagagga gcagtacaac tctacctata gggtggtgag cgtgctgaca 2580
gtgctgcacc aggactggct gaacggcaag gagtataagt gcaaggtgag caataaggcc 2640
ctgcctgccc caatcgagaa gacaatctcc aaggccaagg gccagccaag agagccccag 2700
gtgtacaccc tgccccctag cagggatgag ctgacaaaga accaggtgtc cctgacctgt 2760
ctggtgaagg gcttttatcc ctccgacatc gccgtggagt gggagtctaa tggccagcct 2820
gagaataact acaagacaac cccacccgtg ctggattctg acggcagctt ctttctgtat 2880
tctaagctga ccgtggacaa gagcaggtgg cagcagggca acgtgttcag ctgctccgtg 2940
atgcacgaag cactgcacaa tcactacacc cagaaatcac tgtcactgag ccctggcaaa 3000
3000
<210> 67
<211> 3036
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 67
gctgggcctc gggctccttg tgctgccgcc tgcacatgtg caggcgattc cctggactgc 60
ggcggcagag gcctggccgc cctgcctggc gatctgccat cctggacccg gagcctgaac 120
ctgagctaca acaagctgag cgagatcgat cccgccggct ttgaggacct gcctaacctg 180
caggaggtgt atctgaacaa taacgagctg accgcggtac catccctggg cgctgcttca 240
tcacatgtcg tctctctctt tctgcagcac aacaagattc gcagcgtgga ggggagccag 300
ctgaaggcct acctttcctt agaagtgtta gatctgagtt tgaacaacat cacggaagtg 360
cggaacacct gctttccaca cggaccgcct ataaaggagc tcaacctggc aggcaatcgg 420
attggcaccc tggagttggg agcatttgat ggtctgtcac ggtcgctgct aactcttcgc 480
ctgagcaaaa acaggatcac ccagcttcct gtaagagcat tcaagctacc caggctgaca 540
caactggacc tcaatcggaa caggattcgg ctgatagagg gcctcacctt ccaggggctc 600
aacagcttgg aggtgctgaa gcttcagcga aacaacatca gcaaactgac agatggggcc 660
ttctggggac tgtccaagat gcatgtgctg cacctggagt acaacagcct ggtagaagtg 720
aacagcggct cgctctacgg cctcacggcc ctgcatcagc tccacctcag caacaattcc 780
atcgctcgca ttcaccgcaa gggctggagc ttctgccaga agctgcatga gttggtcctg 840
tccttcaaca acctgacacg gctggacgag gagagcctgg ccgagctgag cagcctgagt 900
gtcctgcgtc tcagccacaa ttccatcagc cacattgcgg agggtgcctt caagggactc 960
aggagcctgc gagtcttgga tctggaccat aacgagattt cgggcacaat agaggacacg 1020
agcggcgcct tctcagggct cgacagcctc agcaagctga ctctgtttgg aaacaagatc 1080
aagtctgtgg ctaagagagc attctcgggg ctggaaggcc tggagcacct gaaccttgga 1140
gggaatgcga tcagatctgt ccagtttgat gcctttgtga agatgaagaa tcttaaagag 1200
ctccatatca gcagcgacag cttcctgtgt gactgccagc tgaagtggct gcccccgtgg 1260
ctaattggca ggatgctgca ggcctttgtg acagccacct gtgcccaccc agaatcactg 1320
aagggtcaga gcattttctc tgtgccacca gagagtttcg tgtgcgatga cttcctgaag 1380
ccacagatca tcacccagcc agaaaccacc atggctatgg tgggcaagga catccggttt 1440
acatgctcag cagccagcag cagcagctcc cccatgacct ttgcctggaa gaaagacaat 1500
gaagtcctga ccaatgcaga catggagaac tttgtccacg tccacgcgca ggacggggaa 1560
gtgatggagt acaccaccat cctgcacctc cgtcaggtca ctttcgggca cgagggccgc 1620
taccaatgtg tcatcaccaa ccactttggc tccacctatt cacataaggc caggctcacc 1680
gtgaatgtgt tgccatcatt caccaaaacg ccccacgaca taaccatccg gaccaccacc 1740
gtggcccgcc tcgaatgtgc tgccacaggt cacccaaacc ctcagattgc ctggcagaag 1800
gatggaggca cggatttccc cgctgcccgt gagcgacgca tgcatgtcat gccggatgac 1860
gacgtgtttt tcatcactga tgtgaaaata gatgacgcag gggtttacag ctgtactgct 1920
cagaactcag ccggttctat ttcagctaat gccaccctga ctgtcctaga gaccccatcc 1980
ttggtggtcc ccttggaaga ccgtgtggta tctgtgggag aaacagtggc cctccaatgc 2040
aaagccacgg ggaaccctcc gccccgcatc acctggttca agggggaccg cccgctgagc 2100
ctcactgagc ggcaccacct gacccctgac aaccagctcc tggtggttca gaacgtggtg 2160
gcagaggatg cgggccgata tacctgtgag atgtccaaca ccctgggcac ggagcgagct 2220
cacagccagc tgagcgtcct gcccgcagca ggctgcagga aggatgggac cacggtaggc 2280
atcttcggcg gtggcggatc ccgcaacacc ggccgcggcg gcgaggagaa gaagaaggag 2340
aaggagaagg aggagcagga ggagcgcgag accaagaccc ccgagtgccc cagccacacc 2400
cagcccctgg gcgtgttcct gtttcctcca aagcccaagg acacactgat gatctccagg 2460
acaccagagg tgacctgcgt ggtggtggac gtgagccacg aggaccccga ggtgaagttc 2520
aactggtacg tggatggcgt ggaggtgcac aatgccaaga ccaagcccag agaggagcag 2580
tacaactcta cctatagggt ggtgagcgtg ctgacagtgc tgcaccagga ctggctgaac 2640
ggcaaggagt ataagtgcaa ggtgagcaat aaggccctgc ctgccccaat cgagaagaca 2700
atctccaagg ccaagggcca gccaagagag ccccaggtgt acaccctgcc ccctagcagg 2760
gatgagctga caaagaacca ggtgtccctg acctgtctgg tgaagggctt ttatccctcc 2820
gacatcgccg tggagtggga gtctaatggc cagcctgaga ataactacaa gacaacccca 2880
cccgtgctgg attctgacgg cagcttcttt ctgtattcta agctgaccgt ggacaagagc 2940
aggtggcagc agggcaacgt gttcagctgc tccgtgatgc acgaagcact gcacaatcac 3000
tacacccaga aatcactgtc actgagccct ggcaaa 3036
<210> 68
<211> 2997
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 68
gctgggcctc gggctccttg tgctgccgcc tgcacatgtg caggcgattc cctggactgc 60
ggcggcagag gcctggccgc cctgcctggc gatctgccat cctggacccg gagcctgaac 120
ctgagctaca acaagctgag cgagatcgat cccgccggct ttgaggacct gcctaacctg 180
caggaggtgt atctgaacaa taacgagctg accgcggtac catccctggg cgctgcttca 240
tcacatgtcg tctctctctt tctgcagcac aacaagattc gcagcgtgga ggggagccag 300
ctgaaggcct acctttcctt agaagtgtta gatctgagtt tgaacaacat cacggaagtg 360
cggaacacct gctttccaca cggaccgcct ataaaggagc tcaacctggc aggcaatcgg 420
attggcaccc tggagttggg agcatttgat ggtctgtcac ggtcgctgct aactcttcgc 480
ctgagcaaaa acaggatcac ccagcttcct gtaagagcat tcaagctacc caggctgaca 540
caactggacc tcaatcggaa caggattcgg ctgatagagg gcctcacctt ccaggggctc 600
aacagcttgg aggtgctgaa gcttcagcga aacaacatca gcaaactgac agatggggcc 660
ttctggggac tgtccaagat gcatgtgctg cacctggagt acaacagcct ggtagaagtg 720
aacagcggct cgctctacgg cctcacggcc ctgcatcagc tccacctcag caacaattcc 780
atcgctcgca ttcaccgcaa gggctggagc ttctgccaga agctgcatga gttggtcctg 840
tccttcaaca acctgacacg gctggacgag gagagcctgg ccgagctgag cagcctgagt 900
gtcctgcgtc tcagccacaa ttccatcagc cacattgcgg agggtgcctt caagggactc 960
aggagcctgc gagtcttgga tctggaccat aacgagattt cgggcacaat agaggacacg 1020
agcggcgcct tctcagggct cgacagcctc agcaagctga ctctgtttgg aaacaagatc 1080
aagtctgtgg ctaagagagc attctcgggg ctggaaggcc tggagcacct gaaccttgga 1140
gggaatgcga tcagatctgt ccagtttgat gcctttgtga agatgaagaa tcttaaagag 1200
ctccatatca gcagcgacag cttcctgtgt gactgccagc tgaagtggct gcccccgtgg 1260
ctaattggca ggatgctgca ggcctttgtg acagccacct gtgcccaccc agaatcactg 1320
aagggtcaga gcattttctc tgtgccacca gagagtttcg tgtgcgatga cttcctgaag 1380
ccacagatca tcacccagcc agaaaccacc atggctatgg tgggcaagga catccggttt 1440
acatgctcag cagccagcag cagcagctcc cccatgacct ttgcctggaa gaaagacaat 1500
gaagtcctga ccaatgcaga catggagaac tttgtccacg tccacgcgca ggacggggaa 1560
gtgatggagt acaccaccat cctgcacctc cgtcaggtca ctttcgggca cgagggccgc 1620
taccaatgtg tcatcaccaa ccactttggc tccacctatt cacataaggc caggctcacc 1680
gtgaatgtgt tgccatcatt caccaaaacg ccccacgaca taaccatccg gaccaccacc 1740
gtggcccgcc tcgaatgtgc tgccacaggt cacccaaacc ctcagattgc ctggcagaag 1800
gatggaggca cggatttccc cgctgcccgt gagcgacgca tgcatgtcat gccggatgac 1860
gacgtgtttt tcatcactga tgtgaaaata gatgacgcag gggtttacag ctgtactgct 1920
cagaactcag ccggttctat ttcagctaat gccaccctga ctgtcctaga gaccccatcc 1980
ttggtggtcc ccttggaaga ccgtgtggta tctgtgggag aaacagtggc cctccaatgc 2040
aaagccacgg ggaaccctcc gccccgcatc acctggttca agggggaccg cccgctgagc 2100
ctcactgagc ggcaccacct gacccctgac aaccagctcc tggtggttca gaacgtggtg 2160
gcagaggatg cgggccgata tacctgtgag atgtccaaca ccctgggcac ggagcgagct 2220
cacagccagc tgagcgtcct gcccgcagca ggctgcagga aggatgggac cacggtaggc 2280
atcttcggcg gtggcggatc cgaaccgaaa tcttctgaca aaacccacac ctctccgccg 2340
tctccggctc cggaactgct gggtggttct tctgttttcc tgtttcctcc aaagcccaag 2400
gacacactga tgatctccag gacaccagag gtgacctgcg tggtggtgga cgtgagccac 2460
gaggaccccg aggtgaagtt caactggtac gtggatggcg tggaggtgca caatgccaag 2520
accaagccca gagaggagca gtacaactct acctataggg tggtgagcgt gctgacagtg 2580
ctgcaccagg actggctgaa cggcaaggag tataagtgca aggtgagcaa taaggccctg 2640
cctgccccaa tcgagaagac aatctccaag gccaagggcc agccaagaga gccccaggtg 2700
tacaccctgc cccctagcag ggatgagctg acaaagaacc aggtgtccct gacctgtctg 2760
gtgaagggct tttatccctc cgacatcgcc gtggagtggg agtctaatgg ccagcctgag 2820
aataactaca agacaacccc acccgtgctg gattctgacg gcagcttctt tctgtattct 2880
aagctgaccg tggacaagag caggtggcag cagggcaacg tgttcagctg ctccgtgatg 2940
cacgaagcac tgcacaatca ctacacccag aaatcactgt cactgagccc tggcaaa 2997
<210> 69
<211> 997
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 69
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Gly Gly Gly Gly Ser Glu Pro Lys
755 760 765
Ser Ser Asp Lys Thr His Thr Ser Pro Pro Cys Pro Ala Pro Glu Leu
770 775 780
Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
785 790 795 800
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
805 810 815
Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val
820 825 830
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser
835 840 845
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
850 855 860
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala
865 870 875 880
Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
885 890 895
Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln
900 905 910
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
915 920 925
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
930 935 940
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu
945 950 955 960
Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser
965 970 975
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
980 985 990
Leu Ser Pro Gly Lys
995
<210> 70
<211> 998
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 70
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Gly Gly Gly Gly Ser Glu Pro Arg
755 760 765
Gly Pro Thr Ile Lys Pro Cys Pro Pro Cys Lys Cys Pro Ala Pro Asn
770 775 780
Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp
785 790 795 800
Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
805 810 815
Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly
820 825 830
Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn
835 840 845
Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp
850 855 860
Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro
865 870 875 880
Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
885 890 895
Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn
900 905 910
Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile
915 920 925
Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr
930 935 940
Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys
945 950 955 960
Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys
965 970 975
Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
980 985 990
Ser Leu Ser Pro Gly Lys
995
<210> 71
<211> 1010
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 71
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Gly Gly Gly Gly Ser Arg Asn Thr
755 760 765
Gly Arg Gly Gly Glu Glu Lys Lys Lys Glu Lys Glu Lys Glu Glu Gln
770 775 780
Glu Glu Arg Glu Thr Lys Thr Pro Glu Cys Pro Ser His Thr Gln Pro
785 790 795 800
Leu Gly Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile
805 810 815
Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu
820 825 830
Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
835 840 845
Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg
850 855 860
Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
865 870 875 880
Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu
885 890 895
Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr
900 905 910
Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu
915 920 925
Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp
930 935 940
Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val
945 950 955 960
Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp
965 970 975
Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His
980 985 990
Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro
995 1000 1005
Gly Lys
1010
<210> 72
<211> 997
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 72
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Gly Gly Gly Gly Ser Glu Pro Lys
755 760 765
Ser Ser Asp Lys Thr His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu
770 775 780
Leu Gly Gly Ser Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
785 790 795 800
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
805 810 815
Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val
820 825 830
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser
835 840 845
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
850 855 860
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala
865 870 875 880
Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
885 890 895
Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln
900 905 910
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
915 920 925
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
930 935 940
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu
945 950 955 960
Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser
965 970 975
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
980 985 990
Leu Ser Pro Gly Lys
995
<210> 73
<211> 2991
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 73
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
ggcggtggcg gatccgagcc aaagtcctct gataagacac acacctctcc accatgccca 2340
gcaccagagc tgctgggagg accaagcgtg ttcctgtttc ctccaaagcc caaggacaca 2400
ctgatgatct ccaggacacc agaggtgacc tgcgtggtgg tggacgtgag ccacgaggac 2460
cccgaggtga agttcaactg gtacgtggat ggcgtggagg tgcacaatgc caagaccaag 2520
cccagagagg agcagtacaa ctctacctat agggtggtga gcgtgctgac agtgctgcac 2580
caggactggc tgaacggcaa ggagtataag tgcaaggtga gcaataaggc cctgcctgcc 2640
ccaatcgaga agacaatctc caaggccaag ggccagccaa gagagcccca ggtgtacacc 2700
ctgcccccta gcagggatga gctgacaaag aaccaggtgt ccctgacctg tctggtgaag 2760
ggcttttatc cctccgacat cgccgtggag tgggagtcta atggccagcc tgagaataac 2820
tacaagacaa ccccacccgt gctggattct gacggcagct tctttctgta ttctaagctg 2880
accgtggaca agagcaggtg gcagcagggc aacgtgttca gctgctccgt gatgcacgaa 2940
gcactgcaca atcactacac ccagaaatca ctgtcactga gccctggcaa a 2991
<210> 74
<211> 2994
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 74
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
ggcggtggcg gatccgagcc tcggggccct accatcaagc cctgcccccc ttgcaagtgc 2340
cctgccccta atctgctggg cggaccctcc gtgttcctgt ttcctccaaa gcccaaggac 2400
acactgatga tctccaggac accagaggtg acctgcgtgg tggtggacgt gagccacgag 2460
gaccccgagg tgaagttcaa ctggtacgtg gatggcgtgg aggtgcacaa tgccaagacc 2520
aagcccagag aggagcagta caactctacc tatagggtgg tgagcgtgct gacagtgctg 2580
caccaggact ggctgaacgg caaggagtat aagtgcaagg tgagcaataa ggccctgcct 2640
gccccaatcg agaagacaat ctccaaggcc aagggccagc caagagagcc ccaggtgtac 2700
accctgcccc ctagcaggga tgagctgaca aagaaccagg tgtccctgac ctgtctggtg 2760
aagggctttt atccctccga catcgccgtg gagtgggagt ctaatggcca gcctgagaat 2820
aactacaaga caaccccacc cgtgctggat tctgacggca gcttctttct gtattctaag 2880
ctgaccgtgg acaagagcag gtggcagcag ggcaacgtgt tcagctgctc cgtgatgcac 2940
gaagcactgc acaatcacta cacccagaaa tcactgtcac tgagccctgg caaa 2994
<210> 75
<211> 3030
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 75
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
ggcggtggcg gatcccgcaa caccggccgc ggcggcgagg agaagaagaa ggagaaggag 2340
aaggaggagc aggaggagcg cgagaccaag acccccgagt gccccagcca cacccagccc 2400
ctgggcgtgt tcctgtttcc tccaaagccc aaggacacac tgatgatctc caggacacca 2460
gaggtgacct gcgtggtggt ggacgtgagc cacgaggacc ccgaggtgaa gttcaactgg 2520
tacgtggatg gcgtggaggt gcacaatgcc aagaccaagc ccagagagga gcagtacaac 2580
tctacctata gggtggtgag cgtgctgaca gtgctgcacc aggactggct gaacggcaag 2640
gagtataagt gcaaggtgag caataaggcc ctgcctgccc caatcgagaa gacaatctcc 2700
aaggccaagg gccagccaag agagccccag gtgtacaccc tgccccctag cagggatgag 2760
ctgacaaaga accaggtgtc cctgacctgt ctggtgaagg gcttttatcc ctccgacatc 2820
gccgtggagt gggagtctaa tggccagcct gagaataact acaagacaac cccacccgtg 2880
ctggattctg acggcagctt ctttctgtat tctaagctga ccgtggacaa gagcaggtgg 2940
cagcagggca acgtgttcag ctgctccgtg atgcacgaag cactgcacaa tcactacacc 3000
cagaaatcac tgtcactgag ccctggcaaa 3030
<210> 76
<211> 2991
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 76
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
ggcggtggcg gatccgaacc gaaatcttct gacaaaaccc acacctctcc gccgtctccg 2340
gctccggaac tgctgggtgg ttcttctgtt ttcctgtttc ctccaaagcc caaggacaca 2400
ctgatgatct ccaggacacc agaggtgacc tgcgtggtgg tggacgtgag ccacgaggac 2460
cccgaggtga agttcaactg gtacgtggat ggcgtggagg tgcacaatgc caagaccaag 2520
cccagagagg agcagtacaa ctctacctat agggtggtga gcgtgctgac agtgctgcac 2580
caggactggc tgaacggcaa ggagtataag tgcaaggtga gcaataaggc cctgcctgcc 2640
ccaatcgaga agacaatctc caaggccaag ggccagccaa gagagcccca ggtgtacacc 2700
ctgcccccta gcagggatga gctgacaaag aaccaggtgt ccctgacctg tctggtgaag 2760
ggcttttatc cctccgacat cgccgtggag tgggagtcta atggccagcc tgagaataac 2820
tacaagacaa ccccacccgt gctggattct gacggcagct tctttctgta ttctaagctg 2880
accgtggaca agagcaggtg gcagcagggc aacgtgttca gctgctccgt gatgcacgaa 2940
gcactgcaca atcactacac ccagaaatca ctgtcactga gccctggcaa a 2991
<210> 77
<211> 997
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 77
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Gly Gly Gly Gly Ser Glu Pro Lys
755 760 765
Ser Ser Asp Lys Thr His Thr Ser Pro Pro Cys Pro Ala Pro Glu Leu
770 775 780
Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val
785 790 795 800
Leu Met Ile Ser Leu Ser Pro Ile Val Thr Cys Val Val Val Asp Val
805 810 815
Ser Glu Asp Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val
820 825 830
Glu Val His Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser
835 840 845
Thr Leu Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met
850 855 860
Ser Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala
865 870 875 880
Pro Ile Glu Arg Thr Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro
885 890 895
Gln Val Tyr Val Leu Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln
900 905 910
Val Thr Leu Thr Cys Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr
915 920 925
Val Glu Trp Thr Asn Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr
930 935 940
Glu Pro Val Leu Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu
945 950 955 960
Arg Val Glu Lys Lys Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser
965 970 975
Val Val His Glu Gly Leu His Asn His His Thr Thr Lys Ser Phe Ser
980 985 990
Arg Thr Pro Gly Lys
995
<210> 78
<211> 998
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 78
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Gly Gly Gly Gly Ser Glu Pro Arg
755 760 765
Gly Pro Thr Ile Lys Pro Cys Pro Pro Cys Lys Cys Pro Ala Pro Asn
770 775 780
Leu Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Ile Lys Asp
785 790 795 800
Val Leu Met Ile Ser Leu Ser Pro Ile Val Thr Cys Val Val Val Asp
805 810 815
Val Ser Glu Asp Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn
820 825 830
Val Glu Val His Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn
835 840 845
Ser Thr Leu Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp
850 855 860
Met Ser Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro
865 870 875 880
Ala Pro Ile Glu Arg Thr Ile Ser Lys Pro Lys Gly Ser Val Arg Ala
885 890 895
Pro Gln Val Tyr Val Leu Pro Pro Pro Glu Glu Glu Met Thr Lys Lys
900 905 910
Gln Val Thr Leu Thr Cys Met Val Thr Asp Phe Met Pro Glu Asp Ile
915 920 925
Tyr Val Glu Trp Thr Asn Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn
930 935 940
Thr Glu Pro Val Leu Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys
945 950 955 960
Leu Arg Val Glu Lys Lys Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys
965 970 975
Ser Val Val His Glu Gly Leu His Asn His His Thr Thr Lys Ser Phe
980 985 990
Ser Arg Thr Pro Gly Lys
995
<210> 79
<211> 1010
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 79
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Gly Gly Gly Gly Ser Arg Asn Thr
755 760 765
Gly Arg Gly Gly Glu Glu Lys Lys Lys Glu Lys Glu Lys Glu Glu Gln
770 775 780
Glu Glu Arg Glu Thr Lys Thr Pro Glu Cys Pro Ser His Thr Gln Pro
785 790 795 800
Leu Gly Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile
805 810 815
Ser Leu Ser Pro Ile Val Thr Cys Val Val Val Asp Val Ser Glu Asp
820 825 830
Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His
835 840 845
Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu Arg
850 855 860
Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys
865 870 875 880
Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu
885 890 895
Arg Thr Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val Tyr
900 905 910
Val Leu Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr Leu
915 920 925
Thr Cys Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu Trp
930 935 940
Thr Asn Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val
945 950 955 960
Leu Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu
965 970 975
Lys Lys Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val His
980 985 990
Glu Gly Leu His Asn His His Thr Thr Lys Ser Phe Ser Arg Thr Pro
995 1000 1005
Gly Lys
1010
<210> 80
<211> 997
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 80
Ala Gln Ala Gly Pro Arg Ala Pro Cys Ala Ala Ala Cys Thr Cys Ala
1 5 10 15
Gly Asp Ser Leu Asp Cys Ser Gly Arg Gly Leu Ala Thr Leu Pro Arg
20 25 30
Asp Leu Pro Ser Trp Thr Arg Ser Leu Asn Leu Ser Tyr Asn Arg Leu
35 40 45
Ser Glu Ile Asp Ser Ala Ala Phe Glu Asp Leu Thr Asn Leu Gln Glu
50 55 60
Val Tyr Leu Asn Ser Asn Glu Leu Thr Ala Ile Pro Ser Leu Gly Ala
65 70 75 80
Ala Ser Ile Gly Val Val Ser Leu Phe Leu Gln His Asn Lys Ile Leu
85 90 95
Ser Val Asp Gly Ser Gln Leu Lys Ser Tyr Leu Ser Leu Glu Val Leu
100 105 110
Asp Leu Ser Ser Asn Asn Ile Thr Glu Ile Arg Ser Ser Cys Phe Pro
115 120 125
Asn Gly Leu Arg Ile Arg Glu Leu Asn Leu Ala Ser Asn Arg Ile Ser
130 135 140
Ile Leu Glu Ser Gly Ala Phe Asp Gly Leu Ser Arg Ser Leu Leu Thr
145 150 155 160
Leu Arg Leu Ser Lys Asn Arg Ile Thr Gln Leu Pro Val Lys Ala Phe
165 170 175
Lys Leu Pro Arg Leu Thr Gln Leu Asp Leu Asn Arg Asn Arg Ile Arg
180 185 190
Leu Ile Glu Gly Leu Thr Phe Gln Gly Leu Asp Ser Leu Glu Val Leu
195 200 205
Arg Leu Gln Arg Asn Asn Ile Ser Arg Leu Thr Asp Gly Ala Phe Trp
210 215 220
Gly Leu Ser Lys Met His Val Leu His Leu Glu Tyr Asn Ser Leu Val
225 230 235 240
Glu Val Asn Ser Gly Ser Leu Tyr Gly Leu Thr Ala Leu His Gln Leu
245 250 255
His Leu Ser Asn Asn Ser Ile Ser Arg Ile Gln Arg Asp Gly Trp Ser
260 265 270
Phe Cys Gln Lys Leu His Glu Leu Ile Leu Ser Phe Asn Asn Leu Thr
275 280 285
Arg Leu Asp Glu Glu Ser Leu Ala Glu Leu Ser Ser Leu Ser Ile Leu
290 295 300
Arg Leu Ser His Asn Ala Ile Ser His Ile Ala Glu Gly Ala Phe Lys
305 310 315 320
Gly Leu Lys Ser Leu Arg Val Leu Asp Leu Asp His Asn Glu Ile Ser
325 330 335
Gly Thr Ile Glu Asp Thr Ser Gly Ala Phe Thr Gly Leu Asp Asn Leu
340 345 350
Ser Lys Leu Thr Leu Phe Gly Asn Lys Ile Lys Ser Val Ala Lys Arg
355 360 365
Ala Phe Ser Gly Leu Glu Ser Leu Glu His Leu Asn Leu Gly Glu Asn
370 375 380
Ala Ile Arg Ser Val Gln Phe Asp Ala Phe Ala Lys Met Lys Asn Leu
385 390 395 400
Lys Glu Leu Tyr Ile Ser Ser Glu Ser Phe Leu Cys Asp Cys Gln Leu
405 410 415
Lys Trp Leu Pro Pro Trp Leu Met Gly Arg Met Leu Gln Ala Phe Val
420 425 430
Thr Ala Thr Cys Ala His Pro Glu Ser Leu Lys Gly Gln Ser Ile Phe
435 440 445
Ser Val Leu Pro Asp Ser Phe Val Cys Asp Asp Phe Pro Lys Pro Gln
450 455 460
Ile Ile Thr Gln Pro Glu Thr Thr Met Ala Val Val Gly Lys Asp Ile
465 470 475 480
Arg Phe Thr Cys Ser Ala Ala Ser Ser Ser Ser Ser Pro Met Thr Phe
485 490 495
Ala Trp Lys Lys Asp Asn Glu Val Leu Ala Asn Ala Asp Met Glu Asn
500 505 510
Phe Ala His Val Arg Ala Gln Asp Gly Glu Val Met Glu Tyr Thr Thr
515 520 525
Ile Leu His Leu Arg His Val Thr Phe Gly His Glu Gly Arg Tyr Gln
530 535 540
Cys Ile Ile Thr Asn His Phe Gly Ser Thr Tyr Ser His Lys Ala Arg
545 550 555 560
Leu Thr Val Asn Val Leu Pro Ser Phe Thr Lys Ile Pro His Asp Ile
565 570 575
Ala Ile Arg Thr Gly Thr Thr Ala Arg Leu Glu Cys Ala Ala Thr Gly
580 585 590
His Pro Asn Pro Gln Ile Ala Trp Gln Lys Asp Gly Gly Thr Asp Phe
595 600 605
Pro Ala Ala Arg Glu Arg Arg Met His Val Met Pro Asp Asp Asp Val
610 615 620
Phe Phe Ile Thr Asp Val Lys Ile Asp Asp Met Gly Val Tyr Ser Cys
625 630 635 640
Thr Ala Gln Asn Ser Ala Gly Ser Val Ser Ala Asn Ala Thr Leu Thr
645 650 655
Val Leu Glu Thr Pro Ser Leu Ala Val Pro Leu Glu Asp Arg Val Val
660 665 670
Thr Val Gly Glu Thr Val Ala Phe Gln Cys Lys Ala Thr Gly Ser Pro
675 680 685
Thr Pro Arg Ile Thr Trp Leu Lys Gly Gly Arg Pro Leu Ser Leu Thr
690 695 700
Glu Arg His His Phe Thr Pro Gly Asn Gln Leu Leu Val Val Gln Asn
705 710 715 720
Val Met Ile Asp Asp Ala Gly Arg Tyr Thr Cys Glu Met Ser Asn Pro
725 730 735
Leu Gly Thr Glu Arg Ala His Ser Gln Leu Ser Ile Leu Pro Thr Pro
740 745 750
Gly Cys Arg Lys Asp Gly Thr Thr Gly Gly Gly Gly Ser Glu Pro Lys
755 760 765
Ser Ser Asp Lys Thr His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu
770 775 780
Leu Gly Gly Ser Ser Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val
785 790 795 800
Leu Met Ile Ser Leu Ser Pro Ile Val Thr Cys Val Val Val Asp Val
805 810 815
Ser Glu Asp Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val
820 825 830
Glu Val His Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser
835 840 845
Thr Leu Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met
850 855 860
Ser Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala
865 870 875 880
Pro Ile Glu Arg Thr Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro
885 890 895
Gln Val Tyr Val Leu Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln
900 905 910
Val Thr Leu Thr Cys Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr
915 920 925
Val Glu Trp Thr Asn Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr
930 935 940
Glu Pro Val Leu Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu
945 950 955 960
Arg Val Glu Lys Lys Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser
965 970 975
Val Val His Glu Gly Leu His Asn His His Thr Thr Lys Ser Phe Ser
980 985 990
Arg Thr Pro Gly Lys
995
<210> 81
<211> 2991
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 81
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
ggcggtggcg gatccgagcc aaagtcctct gataagacac acacctctcc accatgccca 2340
gcaccagagc tgctgggagg accaagcgtg ttcatcttcc cacccaagat caaggacgtg 2400
ctgatgatct ccctgtcccc catcgtgacc tgcgtggtgg tggacgtgtc cgaggacgac 2460
cccgacgtgc agatcagttg gttcgtgaac aacgtggaag tgcacaccgc ccagacccag 2520
acccacagag aggactacaa ctccaccctg cgggtggtgt ccgccctgcc catccagcac 2580
caggactgga tgtccggcaa agaattcaag tgcaaagtga acaacaagga cctgcctgcc 2640
cccatcgagc ggaccatctc caagcccaag ggctccgtgc gggctcccca ggtgtacgtg 2700
ctgccccctc cagaggaaga gatgaccaag aagcaggtca cactgacctg catggtcacc 2760
gacttcatgc ccgaggacat ctacgtggaa tggaccaaca atggcaagac cgagctgaac 2820
tacaagaaca ccgagcctgt gctggactcc gacggctcct acttcatgta ctccaagctg 2880
cgggtggaaa agaagaactg ggtcgagcgg aactcctact cctgctccgt ggtgcacgag 2940
ggcctgcaca accaccacac caccaagtcc ttctcccgga cccccggcaa a 2991
<210> 82
<211> 2994
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 82
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
ggcggtggcg gatccgagcc tcggggccct accatcaagc cctgcccccc ttgcaagtgc 2340
cctgccccta atctgctggg cggaccctcc gtgttcatct tcccacccaa gatcaaggac 2400
gtgctgatga tctccctgtc ccccatcgtg acctgcgtgg tggtggacgt gtccgaggac 2460
gaccccgacg tgcagatcag ttggttcgtg aacaacgtgg aagtgcacac cgcccagacc 2520
cagacccaca gagaggacta caactccacc ctgcgggtgg tgtccgccct gcccatccag 2580
caccaggact ggatgtccgg caaagaattc aagtgcaaag tgaacaacaa ggacctgcct 2640
gcccccatcg agcggaccat ctccaagccc aagggctccg tgcgggctcc ccaggtgtac 2700
gtgctgcccc ctccagagga agagatgacc aagaagcagg tcacactgac ctgcatggtc 2760
accgacttca tgcccgagga catctacgtg gaatggacca acaatggcaa gaccgagctg 2820
aactacaaga acaccgagcc tgtgctggac tccgacggct cctacttcat gtactccaag 2880
ctgcgggtgg aaaagaagaa ctgggtcgag cggaactcct actcctgctc cgtggtgcac 2940
gagggcctgc acaaccacca caccaccaag tccttctccc ggacccccgg caaa 2994
<210> 83
<211> 3030
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 83
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
ggcggtggcg gatcccgcaa caccggccgc ggcggcgagg agaagaagaa ggagaaggag 2340
aaggaggagc aggaggagcg cgagaccaag acccccgagt gccccagcca cacccagccc 2400
ctgggcgtgt tcatcttccc acccaagatc aaggacgtgc tgatgatctc cctgtccccc 2460
atcgtgacct gcgtggtggt ggacgtgtcc gaggacgacc ccgacgtgca gatcagttgg 2520
ttcgtgaaca acgtggaagt gcacaccgcc cagacccaga cccacagaga ggactacaac 2580
tccaccctgc gggtggtgtc cgccctgccc atccagcacc aggactggat gtccggcaaa 2640
gaattcaagt gcaaagtgaa caacaaggac ctgcctgccc ccatcgagcg gaccatctcc 2700
aagcccaagg gctccgtgcg ggctccccag gtgtacgtgc tgccccctcc agaggaagag 2760
atgaccaaga agcaggtcac actgacctgc atggtcaccg acttcatgcc cgaggacatc 2820
tacgtggaat ggaccaacaa tggcaagacc gagctgaact acaagaacac cgagcctgtg 2880
ctggactccg acggctccta cttcatgtac tccaagctgc gggtggaaaa gaagaactgg 2940
gtcgagcgga actcctactc ctgctccgtg gtgcacgagg gcctgcacaa ccaccacacc 3000
accaagtcct tctcccggac ccccggcaaa 3030
<210> 84
<211> 2991
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 84
gctcaggctg gacctagggc tccttgcgct gccgcctgca cctgtgcagg cgattctctg 60
gactgcagcg gccggggcct ggccacactg cccagggacc tgccttcctg gaccagatct 120
ctgaacctga gctacaatcg gctgtccgag atcgattctg ccgcctttga ggacctgaca 180
aatctgcagg aggtgtatct gaacagcaat gagctgaccg caatcccctc cctgggagca 240
gcctctatcg gcgtggtgag cctgttcctg cagcacaaca agatcctgag cgtggatggc 300
tcccagctga agagctacct gtctctggag gtgctggacc tgagctccaa caatatcacc 360
gagatcagat ctagctgttt tcctaatggc ctgcggatca gagagctgaa cctggcctct 420
aatcggatca gcatcctgga gtccggcgcc ttcgatggcc tgagcagatc cctgctgaca 480
ctgcgcctgt ccaagaaccg gatcacccag ctgcccgtga aggcctttaa gctgcctagg 540
ctgacacagc tggacctgaa ccggaataga atcaggctga tcgagggcct gaccttccag 600
ggcctggata gcctggaggt gctgcgcctg cagcggaaca atatctcccg cctgacagac 660
ggagcatttt ggggcctgtc taagatgcac gtgctgcacc tggagtacaa tagcctggtg 720
gaggtgaact ctggcagcct gtatggcctg accgccctgc accagctgca cctgtccaac 780
aatagcatca gcagaatcca gagggatggc tggtccttct gccagaagct gcacgagctg 840
atcctgtctt ttaacaatct gaccaggctg gacgaggaga gcctggcaga gctgtcctct 900
ctgtccatcc tgcgcctgtc tcacaatgcc atcagccaca tcgccgaggg cgcctttaag 960
ggcctgaaga gcctgagggt gctggatctg gaccacaacg agatctctgg caccatcgag 1020
gatacaagcg gcgccttcac aggcctggac aatctgtcca agctgaccct gtttggcaac 1080
aagatcaagt ctgtggccaa gcgggccttc tctggcctgg agagcctgga gcacctgaac 1140
ctgggcgaga atgccatcag atccgtgcag ttcgatgcct ttgccaagat gaagaatctg 1200
aaggagctgt acatcagctc cgagagcttc ctgtgcgact gtcagctgaa gtggctgcca 1260
ccttggctga tgggaaggat gctgcaggcc tttgtgaccg ccacatgcgc ccacccagag 1320
agcctgaagg gccagagcat cttctccgtg ctgcccgata gcttcgtgtg cgacgatttt 1380
cctaagccac agatcatcac ccagccagag acaacaatgg ccgtggtggg caaggacatc 1440
cggtttacat gttccgccgc ctctagctcc tctagcccca tgaccttcgc ctggaagaag 1500
gataacgagg tgctggccaa tgccgacatg gagaacttcg cccacgtgag agcccaggat 1560
ggcgaagtga tggagtatac cacaatcctg cacctgcggc acgtgacctt tggccacgag 1620
ggcagatacc agtgcatcat cacaaatcac ttcggctcta cctatagcca caaggccagg 1680
ctgacagtga acgtgctgcc tagctttacc aagatcccac acgacatcgc catcagaaca 1740
ggcaccacag caaggctgga gtgtgcagca accggacacc caaaccctca gatcgcatgg 1800
cagaaggatg gaggcacaga cttccctgca gcccgcgaga ggagaatgca cgtgatgcca 1860
gacgatgacg tgttctttat cacagatgtg aagatcgatg acatgggcgt gtactcctgc 1920
accgcacaga acagcgccgg cagcgtgtcc gccaacgcca ccctgaccgt gctggagaca 1980
ccatccctgg ccgtgcccct ggaggacagg gtggtgaccg tgggcgagac agtggccttt 2040
cagtgtaagg ccaccggctc tccaacacca aggatcacct ggctgaaggg cggcaggccc 2100
ctgagcctga cagagcgcca ccacttcacc cctggcaatc agctgctggt ggtgcagaac 2160
gtgatgatcg atgacgccgg caggtataca tgcgagatga gcaatcctct gggcaccgag 2220
agggcacact cccagctgtc tatcctgcct accccaggct gccggaagga tggcaccaca 2280
ggcggtggcg gatccgaacc gaaatcttct gacaaaaccc acacctctcc gccgtctccg 2340
gctccggaac tgctgggtgg ttcttctgtt ttcatcttcc cacccaagat caaggacgtg 2400
ctgatgatct ccctgtcccc catcgtgacc tgcgtggtgg tggacgtgtc cgaggacgac 2460
cccgacgtgc agatcagttg gttcgtgaac aacgtggaag tgcacaccgc ccagacccag 2520
acccacagag aggactacaa ctccaccctg cgggtggtgt ccgccctgcc catccagcac 2580
caggactgga tgtccggcaa agaattcaag tgcaaagtga acaacaagga cctgcctgcc 2640
cccatcgagc ggaccatctc caagcccaag ggctccgtgc gggctcccca ggtgtacgtg 2700
ctgccccctc cagaggaaga gatgaccaag aagcaggtca cactgacctg catggtcacc 2760
gacttcatgc ccgaggacat ctacgtggaa tggaccaaca atggcaagac cgagctgaac 2820
tacaagaaca ccgagcctgt gctggactcc gacggctcct acttcatgta ctccaagctg 2880
cgggtggaaa agaagaactg ggtcgagcgg aactcctact cctgctccgt ggtgcacgag 2940
ggcctgcaca accaccacac caccaagtcc ttctcccgga cccccggcaa a 2991

Claims (21)

1.一种融合蛋白，其包含：
富含亮氨酸和免疫球蛋白样结构域1(Lrig-1)蛋白的细胞外结构域；和
免疫球蛋白Fc区。
2.根据权利要求1所述的融合蛋白，其特征在于，Lrig-1蛋白的细胞外结构域由SEQ IDNO：1或3表示。
3.根据权利要求1所述的融合蛋白，其特征在于，所述免疫球蛋白Fc区包含1-4个选自下组的结构域：CH1、CH2、CH3和CH4结构域。
4.根据权利要求1所述的融合蛋白，其特征在于，所述免疫球蛋白Fc区是源自IgG、IgA、IgD、IgE或IgM的Fc区、重链恒定区2(CH2)、重链恒定区3(CH3)、铰链、其片段、其组合、或包含该组合的杂合Fc。
5.根据权利要求1所述的融合蛋白，其特征在于，所述免疫球蛋白Fc区包括源自IgG、IgA、IgD、IgE或IgM的CH2和CH3结构域。
6.根据权利要求1所述的融合蛋白，其特征在于，所述免疫球蛋白Fc区包括源自IgG1、IgG2、IgG3或IgG4的CH2和CH3结构域。
7.根据权利要求1所述的融合蛋白，其特征在于，所述免疫球蛋白Fc区包括铰链区。
8.根据权利要求7所述的融合蛋白，其特征在于，所述免疫球蛋白Fc区包括源自IgG、IgA、IgM、IgD、IgE或阿巴西普的铰链区。
9.根据权利要求7所述的融合蛋白，其特征在于，所述铰链区包括源自IgG1、IgG2、IgG3、IgG4、IgD或阿巴西普的铰链区。
10.根据权利要求1所述的融合蛋白，其特征在于，所述免疫球蛋白Fc区包括CH2和CH3，它们由SEQ ID NO：5或6表示。
11.根据权利要求1所述的融合蛋白，其特征在于，所述免疫球蛋白Fc区包括由SEQ IDNO：7至10中的任何一个表示的铰链区。
12.根据权利要求1所述的融合蛋白，其特征在于，所述Lrig-1蛋白的细胞外结构域直接连接至免疫球蛋白Fc区的N端或C端。
13.根据权利要求1所述的融合蛋白，其特征在于，所述Lrig-1蛋白的细胞外结构域通过接头与免疫球蛋白Fc区的N端或C端连接。
14.根据权利要求13所述的融合蛋白，其特征在于，所述接头是SEQ ID NO：11表示的肽接头和SEQ ID NO：12表示的肽接头中的至少一个。
15.一种核酸分子，其编码根据权利要求1至14中任一项所述的融合蛋白。
16.一种表达载体，其中插入了根据权利要求15所述的核酸分子。
17.一种宿主细胞系，用根据权利要求16所述的表达载体转染。
18.一种用于预防或治疗癌症的药物组合物，其包含根据权利要求1至14中任一项所述的融合蛋白作为活性成分。
19.根据权利要求18所述的药物组合物，其特征在于，所述癌症是实体癌。
20.根据权利要求18所述的药物组合物，其特征在于，所述癌症是胃癌、肝癌、胶质细胞瘤、卵巢癌、结肠直肠癌、头颈癌、膀胱癌、肾细胞癌、乳腺癌、转移癌、前列腺癌、胰腺癌、黑素瘤或肺癌。
21.一种预防或治疗癌症的方法，包括对个体施用权利要求1至14中任一项所述的融合蛋白的步骤。
CN201980028463.XA 2018-04-26 2019-04-26 新型融合蛋白和用于预防或治疗癌症的包含该融合蛋白的药物组合物 Pending CN112041333A (zh)

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KR102194644B1 (ko) 2020-12-24
US11820801B2 (en) 2023-11-21
KR20190124665A (ko) 2019-11-05
WO2019209078A1 (ko) 2019-10-31
JP2021521817A (ja) 2021-08-30
KR20200144524A (ko) 2020-12-29
KR20230146491A (ko) 2023-10-19
US20210130422A1 (en) 2021-05-06
EP3792276A1 (en) 2021-03-17

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