CN111995458A - Nutrition absorption promoter for activating trunk epidermal cells - Google Patents

Nutrition absorption promoter for activating trunk epidermal cells Download PDF

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Publication number
CN111995458A
CN111995458A CN202010717555.3A CN202010717555A CN111995458A CN 111995458 A CN111995458 A CN 111995458A CN 202010717555 A CN202010717555 A CN 202010717555A CN 111995458 A CN111995458 A CN 111995458A
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Prior art keywords
bacillus
parts
agent
trunk
liquid
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CN202010717555.3A
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Chinese (zh)
Inventor
万鹏
李煌毅
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Chifeng Mengding Biotechnology Co ltd
Beijing Shuanglong Amms Technology Co ltd
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Chifeng Mengding Biotechnology Co ltd
Beijing Shuanglong Amms Technology Co ltd
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Priority to CN202010717555.3A priority Critical patent/CN111995458A/en
Publication of CN111995458A publication Critical patent/CN111995458A/en
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a nutrition absorption promoter for activating epidermal cells of a trunk, belonging to the technical field of plant growth nutrients and comprising the following components in parts by weight: ca (NO)3)230-80 parts of FeSO4·7H210-20 parts of O, KNO320-30 parts of MgSO (MgSO)420-40 parts of NH4H2PO420-40 parts of biological agent and 5-10 parts of biological agent. The nutrition promoter for activating the epidermal cells of the trunk to absorb has the effects of promoting the differentiation of phloem cells and repairing injured trees, and fundamentally solves the problems of withering and death of the injured trees.

Description

Nutrition absorption promoter for activating trunk epidermal cells
Technical Field
The invention relates to the technical field of nutrient for promoting plant growth, in particular to a nutrient absorption promoter for activating epidermal cells of a trunk.
Background
The bark is an important part of the trunk and can be divided into an outer bark, a peripheral bark consisting of a cork, a cork cambium and a cork inner layer and an inner phloem from the outside to the inside. Since cells of the outer and periderm become dead cells after formation, epidermal cells in the bark are mainly cells of the phloem. The bark has the functions of preventing cold, summer heat and diseases and pests, and is mainly used for transporting nutrients. The phloem has a channel in which the nutrients produced by photosynthesis in the leaves are transported to the roots and other organs; some trees are hollow, but still have a vigorous vigor, which is due to the presence of epidermal cells in the phloem of the edges, which are capable of transporting nutrients.
If epidermal cells of phloem are damaged, bark is peeled off in a large area, new epidermal cells are not in time to grow out, and roots die due to the fact that organic nutrients are not available. For example, a tree can cause a great deal of damage after being pruned, transplanted or encountering diseases and insect pests and natural disasters, and if the damage is not well treated, the damage is easily infected with bacteria, so that the growth of the tree is slowly influenced, and even the tree is dead. For example, after a trunk of a tree is damaged, a plurality of greening construction units apply paint or mud to the damaged part, although the paint and mud can play a certain role in protection after being dried, the tree also has a pseudo-life period, the paint or mud is seen on the surface to play a role, the tree seems to survive, but after the engineering transfer, the tree grows badly and slowly dies. The paint and mud contain bacteria, which not only can not play a good role in protection, but also can cause the tree body to be infected with diseases, especially some trees with larger damaged surfaces, if the trees are not repaired in time by adopting a correct method, the trees still die slowly after a period of time.
Therefore, in the case of trees whose trunk is damaged, the key to prevent death or revival is to promote the growth of epidermal cells in phloem, and thus, research on such nutrients is urgently needed.
Disclosure of Invention
In order to solve the above technical problems, it is an object of the present invention to provide an activated trunk epidermal cell absorption nutrition enhancer which can activate epidermal cells in the bark, thereby repairing injured trees and preventing the trees from withering or dying.
In order to achieve the above purpose, the present invention provides the following technical solutions:
an activated trunk epidermal cell absorption nutrition promoter comprises the following components in parts by weight:
Ca(NO3)2 30-80
FeSO4·7H2O 10-20
KNO3 20-30
MgSO4 20-40
NH4H2PO4 20-40
5-10 parts of biological agent.
By adopting the technical scheme, under the premise of meeting basic plant nutrition, biological agents are added, and the biological agents have the effects of secreting plant growth hormone and colonizing or promoting decomposition and absorption of mineral substances, so that the biological agents can cooperate with nutrient components in the original components to provide nutrition required by phloem repair, regulate the form formation of callus, activate phloem cells and promote the growth of tree root systems and branch buds, thereby achieving the effect of repairing injured trees.
Further, the biological agent is a mixed agent consisting of a bacillus subtilis agent, a silicate bacillus agent and a bacillus laterosporus agent.
Furthermore, the mass ratio of the bacillus subtilis to the silicate bacillus to the bacillus laterosporus is 1:1: 1.
Furthermore, the activated trunk epidermal cell absorption nutrition promoter is prepared by mixing the following raw materials in parts by weight:
Ca(NO3)2 60
FeSO4·7H2O 15
KNO3 25
MgSO4 30
NH4H2PO4 30
bacillus subtilis preparation 3
Silicate bacillus agent 3
Bacillus laterosporus agent 3.
Furthermore, the colony number of the bacillus subtilis agent is more than or equal to 1000 hundred million/g; the colony number of the silicate bacillus agent is more than or equal to 600 hundred million/g; the colony number of the bacillus laterosporus agent is more than or equal to 1000 hundred million/g.
The bacillus subtilis has strong colonization ability on plant roots, stems, leaves and other parts; the silicate bacillus agent can decompose potassium and silicon in minerals such as feldspar, mica and the like, can also decompose phosphorus in apatite, and can secrete plant growth stimulin and various enzymes so as to enhance the resistance of crops to certain diseases; the bacillus laterosporus has the effects of promoting the growth of plant roots, enhancing the absorption capacity of the roots, enhancing the metabolism of plants, promoting photosynthesis, strengthening a leaf protective film, resisting pathogenic bacteria and improving the utilization rate of a fertilizer; according to the scheme of the invention, the activation effect of phloem cells is improved and the injured trees are repaired by screening the microbial inoculum and the proportion thereof.
Further, the components also comprise the following traditional Chinese medicine components in parts by weight: 5-9 parts of astragalus powder and 8-12 parts of sophora flavescens powder.
The traditional Chinese medicine materials contain certain fibers, so that certain nutrition can be provided for plant growth, meanwhile, trees in nature can inevitably contaminate bacteria when being blown by wind and dried by the sun, the kuhseng contains kuhseng, so that the radix sophorae flavescentis has a sterilization effect, and the astragalus membranaceus is added because the astragalus membranaceus contains various saponins, flavones, polysaccharides, amino acids and the like, so that cell membranes of germs can be damaged, the sterilization or bacteria contamination prevention effect is achieved, and certain nutrition can be provided for plant growth.
Further, the traditional Chinese medicine comprises the following components in parts by weight: 8 parts of astragalus powder and 10 parts of sophora flavescens powder.
By optimizing the dosage and the proportion of the astragalus and the sophora flavescens, the prepared nutrition promoter for activating the trunk epidermal cells can achieve better effects of sterilization and nutrition supply.
Further, the traditional Chinese medicine components are processed by adopting the following method:
1) inoculating lactobacillus strains into 50 mM MRRS liquid culture medium, fermenting for 24h at 37 ℃ in a gas bath shaker 200rmp, then inoculating the lactobacillus strains into the same liquid culture medium by 3 percent of inoculation amount, and carrying out amplification culture under the same condition to obtain lactobacillus bacteria liquid; inoculating the bacillus strain into 50mL LB culture solution, culturing for 36h at 37 ℃ by using a gas bath shaker 200 rmp; then inoculating the bacillus subtilis into the same liquid culture medium by 3 percent of inoculation amount, and performing amplification culture under the same condition to obtain bacillus liquid; mixing lactobacillus and bacillus liquid according to the proportion of 1: 2;
2) sterilizing radix Sophorae Flavescentis powder and Scutellariae radix powder; inoculating the traditional Chinese medicines into the mixed bacterial liquid according to the mass ratio of 6%, fermenting at 37 ℃ for 72 hours, sterilizing and filtering to obtain fermented liquid medicine;
3) concentrating the fermented liquid, drying, and pulverizing.
By fermenting the astragalus and the radix sophorae flavescentis, on one hand, macromolecular saccharides can be decomposed, on the other hand, the content of effective components can be increased, the contents of saponin, flavone, amino acid and matrine in the fermented traditional Chinese medicine liquid can be increased, and meanwhile, fermentation metabolites beneficial to plant growth can be increased, so that nutrition is provided for tree growth and development.
In summary, the technical scheme provided by the invention has the following beneficial effects:
1. according to the scheme provided by the invention, on the premise of basic nutritional agents, biological agents are added, and the biological agents have the effects of secreting plant growth hormone and colonizing or promoting decomposition and absorption of minerals, so that the biological agents can cooperate with nutritional ingredients in the original components to provide nutrition required by phloem repair, regulate the form formation of callus, activate phloem cells and promote the growth of tree root systems and branch buds, thereby achieving the effect of repairing injured trees.
2. The scheme of the invention also adds Chinese herbal medicine components, has the functions of sterilization and nutrition supply, can eliminate the cause of microbial infection causing dry trees, and supplies nutrition to the trees.
3. According to the scheme of the invention, the lactic acid bacteria and the bacillus are adopted to ferment the Chinese herbal medicine, so that the dissolution of active ingredients and the decomposition of macromolecular substances such as polysaccharide or protein are facilitated in the fermentation process, and the effects of improving the sterilization effect and providing nutrient substances for plant growth are achieved.
4. The invention has the advantages of comprehensive nutrition, easy absorption, convenient preparation and low cost.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1
Weighing the following components in parts by weight, and uniformly mixing:
Ca(NO3)2 30
FeSO4·7H2O 10
KNO3 20
MgSO4 20
NH4H2PO4 20
bacillus subtilis preparation 1
Silicate bacillus agent 2
Bacillus laterosporus 2.
The colony number of the bacillus subtilis agent is more than or equal to 1000 hundred million/g; the colony number of the silicate bacillus agent is more than or equal to 600 hundred million/g; the colony number of the bacillus laterosporus agent is more than or equal to 1000 hundred million/g. Are all commercial products.
Example 2
Ca(NO3)2 40
FeSO4·7H2O 15
KNO3 25
MgSO4 35
NH4H2PO4 25
Bacillus subtilis preparation 2
Silicate bacillus agent 2
Bacillus laterosporus 2.
Example 3
Ca(NO3)2 70
FeSO4·7H2O 18
KNO3 25
MgSO4 25
NH4H2PO4 35
Bacillus subtilis preparation 3
Silicate bacillus agent 2
Bacillus laterosporus 2.
Example 4
Ca(NO3)2 40
FeSO4·7H2O 13
KNO3 13
MgSO4 27
NH4H2PO4 30
Bacillus subtilis preparation 3
Silicate bacillus agent 3
Bacillus laterosporus 2.
Example 5
Ca(NO3)2 60
FeSO4·7H2O 15
KNO3 25
MgSO4 30
NH4H2PO4 30
Bacillus subtilis preparation 3
Silicate bacillus agent 3
Bacillus laterosporus agent 3.
Example 6
Ca(NO3)2 80
FeSO4·7H2O 20
KNO3 30
MgSO4 40
NH4H2PO4 40
Bacillus subtilis preparation 3
Silicate bacillus agent 3
Bacillus laterosporus 4.
Example 7
Except for weighing the components in the embodiment 5, 5kg of astragalus powder and 12kg of sophora flavescens powder are also weighed, and then all the components are uniformly mixed.
Example 8
Weighing 7kg of radix astragali powder and 10kg of radix Sophorae Flavescentis powder in addition to the components in example 5, and mixing the components uniformly
Example 9
Except for weighing the components in the embodiment 5, 9kg of astragalus powder and 8kg of sophora flavescens powder are also weighed, and then all the components are uniformly mixed.
Example 10
1) Inoculating lactobacillus strains into 50 mM MRRS liquid culture medium, fermenting for 24h at 37 ℃ in a gas bath shaker 200rmp, then inoculating the lactobacillus strains into the same liquid culture medium by 3 percent of inoculation amount, and carrying out amplification culture under the same condition to obtain lactobacillus bacteria liquid; inoculating the bacillus strain into 50mL LB culture solution, culturing for 36h at 37 ℃ by using a gas bath shaker 200 rmp; then inoculating the bacillus subtilis into the same liquid culture medium by 3 percent of inoculation amount, and performing amplification culture under the same condition to obtain bacillus liquid; mixing lactobacillus and bacillus liquid according to the proportion of 1: 2;
2) weighing 5kg of astragalus powder and 12kg of sophora flavescens powder, and sterilizing; inoculating 6% of the Chinese medicinal materials into the mixed bacteria liquid, fermenting at 37 deg.C for 72 hr, sterilizing, and filtering to obtain fermented liquid medicine;
3) concentrating the fermented liquid, drying, and pulverizing;
4) the rest components are weighed according to the embodiment 5 and then are evenly mixed with the fermented medicinal powder.
Example 11
1) Inoculating lactobacillus strains into 50 mM MRRS liquid culture medium, fermenting for 24h at 37 ℃ in a gas bath shaker 200rmp, then inoculating the lactobacillus strains into the same liquid culture medium by 3 percent of inoculation amount, and carrying out amplification culture under the same condition to obtain lactobacillus bacteria liquid; inoculating the bacillus strain into 50mL LB culture solution, culturing for 36h at 37 ℃ by using a gas bath shaker 200 rmp; then inoculating the bacillus subtilis into the same liquid culture medium by 3 percent of inoculation amount, and performing amplification culture under the same condition to obtain bacillus liquid; mixing lactobacillus and bacillus liquid according to the proportion of 1: 2;
2) taking 7kg of astragalus powder and 10kg of sophora flavescens powder, and sterilizing; adding 6% of the Chinese medicinal materials into the mixed bacteria liquid, fermenting at 37 deg.C for 72 hr, sterilizing, and filtering to obtain fermented liquid.
3) Concentrating the fermented liquid, drying, and pulverizing;
4) the rest components are weighed according to the embodiment 5 and then are evenly mixed with the fermented medicinal powder.
Example 12
1) Inoculating lactobacillus strains into 50 mM MRRS liquid culture medium, fermenting for 24h at 37 ℃ in a gas bath shaker 200rmp, then inoculating the lactobacillus strains into the same liquid culture medium by 3 percent of inoculation amount, and carrying out amplification culture under the same condition to obtain lactobacillus bacteria liquid; inoculating the bacillus strain into 50mL LB culture solution, culturing for 36h at 37 ℃ by using a gas bath shaker 200 rmp; then inoculating the bacillus subtilis into the same liquid culture medium by 3 percent of inoculation amount, and performing amplification culture under the same condition to obtain bacillus liquid; mixing lactobacillus and bacillus liquid according to the proportion of 1: 2;
2) taking 9kg of astragalus powder and 8kg of sophora flavescens powder, and sterilizing; adding 6% of the Chinese medicinal materials into the mixed bacteria liquid, fermenting at 37 deg.C for 72 hr, sterilizing, and filtering to obtain fermented liquid.
3) Concentrating the fermented liquid, drying, and pulverizing;
4) the other components are weighed according to the embodiment 5 and then mixed with the fermented powder.
Effect test
39 poplar trees with the diameter of 10 +/-1 cm are taken, 4/5 of phloem of the poplar trees are cut along the periphery, the branches are found to be soft or withered after one week, then the test is started, wherein three of the poplar trees form a group, one group uses the commercial urea as a control group, the other 12 groups use the nutrition promoter prepared in the embodiment 1-12 respectively, and the urea and the 12 fertilizers are respectively added with 500 times of water for dilution for standby.
Applying fertilizer by root irrigation, digging an annular ditch with the width of 25-30cm and the depth of 10-20cm at the position 3-4 times the diameter of the trunk of each diseased tree, irrigating the prepared nutrition promoter in the control group and the embodiment with the dosage of 50 kg once every 15 days, and observing the growth condition of the trees.
The specific effect data are as follows:
Figure 884930DEST_PATH_IMAGE001
from the above experiments, it can be seen that the scheme provided by the present invention is significantly better than the control group, wherein the microbial agent is added in the scheme of examples 1-6, the effect of the matching ratio provided by example 5 is the best, the traditional Chinese medicine is added in examples 7-9, the effect of the matching ratio provided by example 8 is the best, the traditional Chinese medicine fermentation product is added in examples 10-12, and the effect of example 11 is the best. The effect tests show that the scheme provided by the invention has obvious effects of activating the plant trunk and reviving the tree.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.

Claims (8)

1. The nutrition absorption enhancer for the activated trunk epidermal cells is characterized by comprising the following components in parts by weight:
Ca(NO3)2 30-80
FeSO4·7H2O 10-20
KNO3 20-30
MgSO4 20-40
NH4H2PO4 20-40
5-10 parts of biological agent.
2. The activated trunk epidermal cell absorption nutrition promoter as claimed in claim 1, wherein the biological agent is a mixed agent consisting of a bacillus subtilis agent, a bacillus silicate agent and a bacillus laterosporus agent.
3. The activated trunk epidermal cell absorption nutrition promoter as claimed in claim 2, wherein the mass ratio of the bacillus subtilis preparation, the silicate bacillus preparation and the sporotrichum preparation is 1:1: 1.
4. The activated trunk epidermal cell absorption nutrition promoter as claimed in claim 1, is prepared by mixing the following raw materials in parts by weight:
Ca(NO3)2 60
FeSO4·7H2O 15
KNO3 25
MgSO4 30
NH4H2PO4 30
bacillus subtilis preparation 3
Silicate bacillus agent 3
Bacillus laterosporus agent 3.
5. The activated trunk epidermal cell absorption nutrition promoter according to any one of claims 2-5, wherein the colony number of the bacillus subtilis agent is more than or equal to 1000 hundred million/g; the colony number of the silicate bacillus agent is more than or equal to 600 hundred million/g; the colony number of the bacillus laterosporus agent is more than or equal to 1000 hundred million/g.
6. The activated trunk epidermal cell absorption nutrition promoter as claimed in claim 5, wherein the components further comprise the following traditional Chinese medicine components in parts by weight: 5-9 parts of astragalus powder and 8-12 parts of sophora flavescens powder.
7. The nutrition enhancer for the absorption of the activated trunk epidermal cells according to claim 6, wherein the traditional Chinese medicine components comprise the following components in parts by weight: 8 parts of astragalus powder and 10 parts of sophora flavescens powder.
8. The nutrient absorption enhancer for the activated trunk epidermal cells of claim 6 or 7, wherein the Chinese medicinal components are processed by the following method:
1) inoculating lactobacillus strains into 50 mM MRRS liquid culture medium, fermenting for 24h at 37 ℃ in a gas bath shaker 200rmp, then inoculating the lactobacillus strains into the same liquid culture medium by 3 percent of inoculation amount, and carrying out amplification culture under the same condition to obtain lactobacillus bacteria liquid; inoculating the bacillus strain into 50mL LB culture solution, culturing for 36h at 37 ℃ by using a gas bath shaker 200 rmp; then inoculating the bacillus subtilis into the same liquid culture medium by 3 percent of inoculation amount, and performing amplification culture under the same condition to obtain bacillus liquid; mixing lactobacillus and bacillus liquid according to the proportion of 1: 2;
2) sterilizing radix Sophorae Flavescentis powder and Scutellariae radix powder; inoculating 6% of the Chinese medicinal materials into the mixed bacteria liquid, fermenting at 37 deg.C for 72 hr, sterilizing, and filtering to obtain fermented liquid medicine;
3) concentrating the fermented liquid, drying, and pulverizing.
CN202010717555.3A 2020-07-23 2020-07-23 Nutrition absorption promoter for activating trunk epidermal cells Withdrawn CN111995458A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114409456A (en) * 2022-02-09 2022-04-29 山东农业大学 Microbial fertilizer and application thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114409456A (en) * 2022-02-09 2022-04-29 山东农业大学 Microbial fertilizer and application thereof

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Application publication date: 20201127