CN111675568A - Nutrient for activating tree trunks - Google Patents
Nutrient for activating tree trunks Download PDFInfo
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- CN111675568A CN111675568A CN202010544967.1A CN202010544967A CN111675568A CN 111675568 A CN111675568 A CN 111675568A CN 202010544967 A CN202010544967 A CN 202010544967A CN 111675568 A CN111675568 A CN 111675568A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Pest Control & Pesticides (AREA)
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Abstract
The invention discloses a nutrient for activating a tree trunk, which comprises the following components in parts by weight: ca (NO)3)235-80 parts of FeSO4·7H2O8-20 parts, KNO315-25 parts of MgSO (MgSO)415-35 parts of NH4H2PO425-35 parts of chitin, 2-4 parts of ethylenediamine tetraacetic acid, 1-3 parts of ethylenediamine tetraacetic acid, 0.005-0.01 part of thiamine, 0.005-0.01 part of pyridoxine, 0.001-0.008 part of indoleacetic acid, 0.005-0.01 part of cytokinin and 0.06-0.1 part of thiaketone. The nutrient for activating the trunk provided by the invention has the effects of promoting the differentiation of phloem cells and repairing injured trees, and fundamentally solves the problems of withering and death of the injured trees.
Description
Technical Field
The invention relates to the technical field of nutrient for promoting plant growth, in particular to a nutrient for activating a tree trunk.
Background
From the outside to the inside, the bark can be divided into an outer bark, a peripheral bark composed of cork, cork cambium and cork inner layer, and an inner phloem. The outer cortex is the outermost dead tissue of the tree, consisting of keratinocytes. The periderm is the part between the phloem and the outer skin, and comprises a general name of the cork, a cork forming layer and a cork inner layer, and the epidermis is peeled off after the periderm is formed. The cork is the main component of the outer layer of the bark, can isolate the water and gas from passing through and has the protection effect on the tree. The cork cambium usually has only one or two layers of cells, which are the tissue of the meristematic growth cork, growing outward into the cork layer and inward into the inner layer of the cork, but the cork cambium at the root is transformed by the pericycle. The inner layer of the suppository is a layer of cells differentiated from the cambium of the cork towards the inside. Phloem is the part of the bark that carries nutrients between the trunk and the pericarp of the xylem.
If epidermal cells of phloem are damaged, bark is peeled off in a large area, new epidermal cells are not in time to grow out, and roots die due to the fact that organic nutrients are not available. For example, a tree can cause a great deal of damage after being pruned, transplanted or encountering diseases and insect pests and natural disasters, if the damage is not well treated, not only new epidermal cells need to grow out, but also bacteria are easily infected, so that the growth of the tree is slowly influenced, and even the tree dies. Some greening construction units smear paint or mud on damaged parts after trunks are damaged, although the paint and mud can play a certain protection role after the trunks are damaged, the trees also have a false life, the paint or mud plays a role on the surface, and the trees seem to survive, but after engineering transfer, the trees grow slowly or die slowly, because the paint and mud contain bacteria, the paint and mud not only can not play a good protection role, but also can possibly cause tree body infection to influence the growth of new epidermal cells, especially some trees with larger damaged surfaces, and the trees often die slowly after a period of time.
Therefore, for trees with damaged trunks, the key to preventing death or resurgence is to effectively promote the growth of epidermal cells of phloem, and how to solve the growth of epidermal cells of injured phloem is a great need.
Disclosure of Invention
In order to solve the above technical problems, it is an object of the present invention to provide a nutrient for activating a trunk, which activates epidermal cells in a bark, thereby repairing an injured tree and preventing the tree from withering or dying.
In order to achieve the above purpose, the present invention provides the following technical solutions:
a nutritional agent for activated trunks comprises the following components in parts by weight:
Ca(NO3)235-80
FeSO4·7H2O 8-20
KNO315-25
MgSO415-35
NH4H2PO425-35
chitin 2-4
Ethylenediaminetetraacetic acid 1-3
Thiamine 0.005-0.01
Pyridoxine 0.005-0.01
Indoleacetic acid 0.001-0.008
Cytokinin 0.005-0.01
0.06-0.1 of thiaketone.
The formula of the technical scheme contains mineral elements such as calcium, iron, magnesium, potassium and the like, vitamin thiamine (B1) and pyridoxine (B6), auxin indoleacetic acid and cytokinin are added at the same time, chelating agent ethylenediamine tetraacetic acid and thiaketone are added as transdermal absorbents, and the components have synergistic effect to promote differentiation of epidermal cells of phloem and regulate the form formation of callus, so that the growth of plant roots and new branch buds is promoted.
Further, the cytokinin is 6-benzylaminopurine.
6-benzylaminopurine is selected as cytokinin because 6-benzylaminopurine is more stable, efficient, inexpensive and has a more stable effect on phloem cells than kinetin and zeatin.
Further, the nutrient for activating the tree trunk comprises the following components in parts by weight:
Ca(NO3)255
FeSO4·7H2O 14
KNO320
MgSO425
NH4H2PO430
chitin 3
Ethylenediaminetetraacetic acid 2
Thiamine 0.008
Pyridoxine 0.008
Indolylacetic acid 0.005
6-benzylaminopurine 0.008
0.08 parts of thiaketone.
In the formula, the content of each component is optimized, the optimal proportion is achieved, and the activating effect of the absorbent nutrient for activating the trunk, which is prepared by adopting the formula, is better.
Further, the components also comprise the following trace elements in parts by weight:
H3BO30.5-0.8
MnSO40.3-0.8
CuSO4·5H2O 0.001-0.005
ZnSO40.001-0.005。
by adding the trace elements on the premise of the original components, the tree can be provided with more comprehensive and sufficient nutrition, the supply of nutrition during or after the activation of epidermal cells is provided, and the growth and development of the tree are promoted.
Further, the trace elements comprise the following components in parts by weight:
H3BO30.6
MnSO40.5
CuSO4·5H2O 0.003
ZnSO40.003。
the optimal using amount of the trace elements is searched by optimizing the using amount of the trace elements.
Further, the components also comprise the following traditional Chinese medicine components in parts by weight: 5-9 parts of astragalus powder and 8-12 parts of sophora flavescens powder.
The traditional Chinese medicine materials contain certain fibers, so that certain nutrition can be provided for plant growth, meanwhile, trees in the nature can be inevitably contaminated by wind and sunshine, the kuhseng contains kuhseng element, and has a sterilization effect, and meanwhile, the astragalus membranaceus is added because the astragalus membranaceus contains various saponins, flavones, polysaccharides, amino acids and the like, so that cell membranes of germs can be damaged, the sterilization or bacteria contamination prevention effect is achieved, and certain nutrition can be provided for plant growth.
Further, the traditional Chinese medicine comprises the following components in parts by weight: 8 parts of astragalus powder and 10 parts of sophora flavescens powder.
The prepared absorbent nutrient for activating the tree trunk achieves better effects of sterilizing and providing nutrition by optimizing the dosage and the proportion of the astragalus and the sophora flavescens.
Further, the traditional Chinese medicine components are processed by adopting the following method:
1) inoculating lactobacillus strains into 50 mM MRRS liquid culture medium, fermenting for 24h at 37 ℃ in a gas bath shaker 200rmp, then inoculating the lactobacillus strains into the same liquid culture medium by 3 percent of inoculation amount, and carrying out amplification culture under the same condition to obtain lactobacillus bacteria liquid; inoculating the bacillus strain into 50mL LB culture solution, culturing for 36h at 37 ℃ by using a gas bath shaker 200 rmp; then inoculating the bacillus subtilis into the same liquid culture medium by 3 percent of inoculation amount, and performing amplification culture under the same condition to obtain bacillus liquid; mixing lactobacillus and bacillus liquid according to the proportion of 1: 2;
2) sterilizing radix Sophorae Flavescentis powder and Scutellariae radix powder; inoculating 6% of the Chinese medicinal materials into the mixed bacteria liquid, fermenting at 37 deg.C for 72 hr, sterilizing, and filtering to obtain fermented liquid medicine;
3) concentrating the fermented liquid, drying, and pulverizing.
By fermenting the astragalus and the radix sophorae flavescentis, on one hand, macromolecular saccharides can be decomposed, on the other hand, the content of effective components can be increased, the contents of saponin, flavone, amino acid and matrine in the fermented traditional Chinese medicine liquid can be increased, and meanwhile, fermentation metabolites beneficial to plant growth can be increased, so that nutrition is provided for tree growth and development.
In summary, the technical scheme provided by the invention has the following beneficial effects:
1. the scheme provided by the invention has the effects of promoting the differentiation of epidermal cells and regulating the morphological formation of callus by chelating trace elements, vitamins, auxin and cytokinin together, and also provides nutrients required in the cell growth process, so that the aim of repairing injured trees is fulfilled, and the problems of withering and death of the injured trees are solved.
2. The scheme of the invention also adds Chinese herbal medicine components, has the functions of sterilization and nutrition supply, can eliminate the cause of microbial infection causing dry trees, and supplies nutrition to the trees.
3. According to the scheme of the invention, the lactic acid bacteria and the bacillus are adopted to ferment the Chinese herbal medicine, so that the dissolution of active ingredients and the decomposition of macromolecular substances such as polysaccharide or protein are facilitated in the fermentation process, and the effects of improving the sterilization effect and providing nutrient substances for plant growth are achieved.
4. The invention has the advantages of comprehensive nutrition, easy absorption, convenient preparation and low cost.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1
Weighing the following components in parts by weight, and uniformly mixing:
Ca(NO3) 35kg
FeSO4·7H2O 8kg
KNO315 kg
MgSO415 kg
NH4H2PO425 kg
chitin 2kg
Ethylenediaminetetraacetic acid 1 kg
Thiamine 0.01 kg
Pyridoxine 0.01 kg
Indolylacetic acid 0.008 kg
6-benzylaminopurine 0.01 kg
0.1 kg of thiaketone.
Example 2
Weighing the following components in parts by weight, and uniformly mixing:
Ca(NO3)255 kg
FeSO4·7H2O 14 kg
KNO320 kg
MgSO425 kg
NH4H2PO430 kg
chitin 3 kg
Ethylenediaminetetraacetic acid 2kg
Thiamine 0.008 kg
Pyridoxine 0.008 kg
Indolylacetic acid 0.005 kg
6-benzylaminopurine 0.008 kg
0.08 kg of thiaketone.
Example 3
Weighing the following components in parts by weight, and uniformly mixing:
Ca(NO3) 80 kg
FeSO4·7H2O 20 kg
KNO325 kg
MgSO435 kg
NH4H2PO435 kg
chitin 4 kg
Ethylenediaminetetraacetic acid 3 kg
Thiamine 0.005 kg
Pyridoxine 0.005 kg
Indolylacetic acid 0.001 kg
6-benzylaminopurine 0.005 kg
0.06 kg of thiaketone.
Example 4
In addition to the components in example 2, the following components were weighed and mixed well:
H3BO30.5 kg
MnSO40.3 kg
CuSO4·5H2O 0.005 kg
ZnSO40.005 kg。
example 5
In addition to the components in example 2, the following components were weighed and mixed well:
H3BO30.6 kg
MnSO40.5 kg
CuSO4·5H2O 0.003 kg
ZnSO40.003 kg。
example 6
In addition to the components in example 2, the following components were weighed and mixed well:
H3BO30.8 kg
MnSO40.8 kg
CuSO4·5H2O 0.001 kg
ZnSO40.001 kg。
example 7
Except for weighing the components in the embodiment 5, 5kg of astragalus powder and 12kg of sophora flavescens powder are also weighed, and then all the components are uniformly mixed.
Example 8
Except for weighing the components in the embodiment 5, 7kg of astragalus powder and 10kg of sophora flavescens powder are also weighed, and then all the components are uniformly mixed.
Example 9
Except for weighing the components in the embodiment 5, 9kg of astragalus powder and 8kg of sophora flavescens powder are also weighed, and then all the components are uniformly mixed.
Example 10
1) Inoculating lactobacillus strains into 50 mM MRRS liquid culture medium, fermenting for 24h at 37 ℃ in a gas bath shaker 200rmp, then inoculating the lactobacillus strains into the same liquid culture medium by 3 percent of inoculation amount, and carrying out amplification culture under the same condition to obtain lactobacillus bacteria liquid; inoculating the bacillus strain into 50mL LB culture solution, culturing for 36h at 37 ℃ by using a gas bath shaker 200 rmp; then inoculating the bacillus subtilis into the same liquid culture medium by 3 percent of inoculation amount, and performing amplification culture under the same condition to obtain bacillus liquid; mixing lactobacillus and bacillus liquid according to the proportion of 1: 2;
2) weighing 5kg of astragalus powder and 12kg of sophora flavescens powder, and sterilizing; inoculating 6% of the Chinese medicinal materials into the mixed bacteria liquid, fermenting at 37 deg.C for 72 hr, sterilizing, and filtering to obtain fermented liquid medicine;
3) concentrating the fermented liquid, drying, and pulverizing;
4) the rest components are weighed according to the embodiment 5 and then are evenly mixed with the fermented medicinal powder.
Example 11
1) Inoculating lactobacillus strains into 50 mM MRRS liquid culture medium, fermenting for 24h at 37 ℃ in a gas bath shaker 200rmp, then inoculating the lactobacillus strains into the same liquid culture medium by 3 percent of inoculation amount, and carrying out amplification culture under the same condition to obtain lactobacillus bacteria liquid; inoculating the bacillus strain into 50mL LB culture solution, culturing for 36h at 37 ℃ by using a gas bath shaker 200 rmp; then inoculating the bacillus subtilis into the same liquid culture medium by 3 percent of inoculation amount, and performing amplification culture under the same condition to obtain bacillus liquid; mixing lactobacillus and bacillus liquid according to the proportion of 1: 2;
2) taking 7kg of astragalus powder and 10kg of sophora flavescens powder, and sterilizing; adding 6% of the Chinese medicinal materials into the mixed bacteria liquid, fermenting at 37 deg.C for 72 hr, sterilizing, and filtering to obtain fermented liquid.
3) Concentrating the fermented liquid, drying, and pulverizing;
4) the rest components are weighed according to the embodiment 5 and then are evenly mixed with the fermented medicinal powder.
Example 12
1) Inoculating lactobacillus strains into 50 mM MRRS liquid culture medium, fermenting for 24h at 37 ℃ in a gas bath shaker 200rmp, then inoculating the lactobacillus strains into the same liquid culture medium by 3 percent of inoculation amount, and carrying out amplification culture under the same condition to obtain lactobacillus bacteria liquid; inoculating the bacillus strain into 50mL LB culture solution, culturing for 36h at 37 ℃ by using a gas bath shaker 200 rmp; then inoculating the bacillus subtilis into the same liquid culture medium by 3 percent of inoculation amount, and performing amplification culture under the same condition to obtain bacillus liquid; mixing lactobacillus and bacillus liquid according to the proportion of 1: 2;
2) taking 9kg of astragalus powder and 8kg of sophora flavescens powder, and sterilizing; adding 6% of the Chinese medicinal materials into the mixed bacteria liquid, fermenting at 37 deg.C for 72 hr, sterilizing, and filtering to obtain fermented liquid.
3) Concentrating the fermented liquid, drying, and pulverizing;
4) the other components are weighed according to the embodiment 5 and then mixed with the fermented powder.
Effect test
Randomly selecting 39 cherry trees of 3 years, peeling off 80% of phloem along the trunk one week, and dividing into one group, wherein one group uses commercially available urea as a control group, and the other 12 groups use the nutritional agents prepared in examples 1-12 respectively, and diluting the 12 fertilizers by 500 times respectively for later use.
And after one week, softening all branches, fertilizing by adopting a root irrigation mode, digging an annular ditch with the width of 25-30cm and the depth of 10-20cm at a position of 70cm of the trunk of each cherry tree, irrigating with 50 kg of nutrient solution once every 15 days, and observing the growth condition of the trees.
The specific effect data are as follows:
from the above experiments, the scheme provided by the invention is obviously superior to that of a control group, wherein in the schemes of examples 1-3, the scheme of example 2 has the best effect, trace elements are added in the schemes of examples 4-6, the proportioning effect provided by example 5 is the best, traditional Chinese medicines are added in examples 7-9, the proportioning effect provided by example 8 is the best, and traditional Chinese medicine fermentation products are added in examples 10-12, and the effect of example 11 is the best. As can be seen from the effect tests, the scheme provided by the invention has an obvious effect of activating the plant trunk.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Claims (8)
1. The nutrient for the activated tree trunks is characterized by comprising the following components in parts by weight:
Ca(NO3)235-80
FeSO4·7H2O 8-20
KNO315-25
MgSO415-35
NH4H2PO425-35
chitin 2-4
Ethylenediaminetetraacetic acid 1-3
Thiamine 0.005-0.01
Pyridoxine 0.005-0.01
Indoleacetic acid 0.001-0.008
Cytokinin 0.005-0.01
0.06-0.1 of thiaketone.
2. The activated tree trunk nutrient of claim 1, wherein the cytokinin is 6-benzylaminopurine.
3. The nutrient for activating trunks according to claim 2, comprising the following components in parts by weight:
Ca(NO3)255
FeSO4·7H2O 14
KNO320
MgSO425
NH4H2PO430
chitin 3
Ethylenediaminetetraacetic acid 2
Thiamine 0.008
Pyridoxine 0.008
Indolylacetic acid 0.005
6-benzylaminopurine 0.008
0.08 parts of thiaketone.
4. The activated tree trunk nutrient according to any one of claims 1 to 3, wherein the composition further comprises trace elements in parts by weight as follows:
H3BO30.5-0.8
MnSO40.3-0.8
CuSO4·5H2O 0.001-0.005
ZnSO40.001-0.005。
5. the nutrient for activating trunks according to claim 4, wherein the trace elements are in parts by weight:
H3BO30.6
MnSO40.5
CuSO4·5H2O 0.003
ZnSO40.003。
6. the nutrient for activating trunks according to claim 5, wherein the components further comprise the following traditional Chinese medicine components in parts by weight: 5-9 parts of astragalus powder and 8-12 parts of sophora flavescens powder.
7. The nutrient for activating trunks according to claim 6, wherein the traditional Chinese medicine components comprise the following components in parts by weight: 8 parts of astragalus powder and 10 parts of sophora flavescens powder.
8. The nutrient for activating trunks according to claim 6 or 7, wherein the traditional Chinese medicine components are processed by a method comprising:
1) inoculating lactobacillus strains into 50 mM MRRS liquid culture medium, fermenting for 24h at 37 ℃ in a gas bath shaker 200rmp, then inoculating the lactobacillus strains into the same liquid culture medium by 3 percent of inoculation amount, and carrying out amplification culture under the same condition to obtain lactobacillus bacteria liquid; inoculating the bacillus strain into 50mL LB culture solution, culturing for 36h at 37 ℃ by using a gas bath shaker 200 rmp; then inoculating the bacillus subtilis into the same liquid culture medium by 3 percent of inoculation amount, and performing amplification culture under the same condition to obtain bacillus liquid; mixing lactobacillus and bacillus liquid according to the proportion of 1: 2;
2) sterilizing radix Sophorae Flavescentis powder and Scutellariae radix powder; inoculating 6% of the Chinese medicinal materials into the mixed bacteria liquid, fermenting at 37 deg.C for 72 hr, sterilizing, and filtering to obtain fermented liquid medicine;
3) concentrating the fermented liquid, drying, and pulverizing.
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Application publication date: 20200918 |