CN114409456A - Microbial fertilizer and application thereof - Google Patents

Microbial fertilizer and application thereof Download PDF

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Publication number
CN114409456A
CN114409456A CN202210122147.2A CN202210122147A CN114409456A CN 114409456 A CN114409456 A CN 114409456A CN 202210122147 A CN202210122147 A CN 202210122147A CN 114409456 A CN114409456 A CN 114409456A
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tomato
treatment
microbial fertilizer
substrate
total
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杨凤娟
黄玲玲
李静
魏珉
周波
谢堃
吴金娟
申太荣
高中强
孟伦
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Shandong Future Biotechnology Co ltd
Shandong Agricultural University
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Shandong Future Biotechnology Co ltd
Shandong Agricultural University
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D1/00Fertilisers containing potassium
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers

Abstract

The invention discloses a microbial fertilizer and application thereof. Belongs to the technical field of bacterial manure. The method comprises the following steps: 7 parts of Brevibacillus laterosporus AMCC 101583 with the preservation number of CGMCC No. 24137; 2 parts of Bacillus subtilis AMCC 100001 with the preservation number of CGMCC No. 6764; 1 part of Bacillus mucilaginosus (Bacillus mucoginosus) AMCC 100035 with the preservation number of CGMCC No.6766 and nutrient solution. The invention has obvious influence on the growth, the yield and the quality of the tomatoes, the activity of matrixase and the number of microorganisms, and is an optimized fertilization formula suitable for the quality cultivation of the fresh-eating tomatoes in the matrix.

Description

Microbial fertilizer and application thereof
Technical Field
The invention relates to the technical field of bacterial fertilizers, in particular to a microbial fertilizer and application thereof.
Background
The tomato (tomato), namely, the tomato, is an annual or perennial herbaceous plant of tubular meshes, solanaceae and tomato, the whole plant grows mucilaginous gland hair and has strong smell, the stem is easy to fall down, the leaf is pinnate and multiple leaves or pinnate deep crack, the total stem of the inflorescence is 2-5 cm long, 3-7 flowers are usually used, the calyx is spoke-shaped, the corolla is spoke-shaped, the berry is spherical or nearly spherical, the pulp is rich in juice, the seed is yellow, and the flower and fruit period is summer and autumn. Tomato is cultivated widely in south America and south and north China. The tomato has rich nutrition and special flavor. Can be eaten raw, boiled, processed tomato paste, juice or canned. China already realizes the large-scale planting of tomatoes.
However, under conventional fertilization, tomato quality and yield are poor.
Therefore, the problem to be solved by the technicians in the field is how to provide a microbial fertilizer to improve the quality of matrix culture tomatoes.
Disclosure of Invention
In view of the above, the invention provides a microbial fertilizer and an application thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
a microbial fertilizer comprising: 7 parts of Brevibacillus laterosporus AMCC 101583 with the preservation number of CGMCC No. 24137; 2 parts of Bacillus subtilis AMCC 100001 with the preservation number of CGMCC No. 6764; 1 part of Bacillus mucilaginosus (Bacillus mucoginosus) AMCC 100035 with the preservation number of CGMCC No.6766 and nutrient solution.
The preservation information is as follows:
brevibacillus laterosporus (Brevibacillus laterosporus) AMCC 101583, deposited in China general microbiological culture Collection center, with the deposition address: the microbial research institute of China academy of sciences No. 3, Xilu No. 1, Beijing, Chaoyang, with the preservation number of CGMCC No.24137 and the preservation time of 2021 year, 12 months and 20 days.
Bacillus subtilis AMCC 100001, preserved in China general microbiological culture Collection center, with the preservation address: the preservation number of the microorganism culture medium is CGMCC No.6764 and the preservation time is 11 months and 01 days in 2012.
Bacillus mucilaginosus (Bacillus licheniformis) AMCC 100035, which is preserved in China general microbiological culture Collection center, with the preservation address: the preservation number of the microorganism culture medium is CGMCC No.6766, and the preservation time is 11 months and 01 days in 2012.
Preferably: the nutrient solution comprises: macroelements of nitrogen, phosphorus, potassium, calcium, magnesium and sulfur with concentrations of 7.7 mmol.L-1、0.7mmol·L-1、4.0mmol·L-1、1.5mmol·L-1、1.0mmol·L-1And 1.0 mmol. L-1(ii) a The trace elements of iron, manganese, copper, zinc, boron and molybdenum have the concentrations of 54 mu mol.L respectively-1、 10μmol·L-1、0.32μmol·L-1、0.77μmol·L-1、46.3μmol·L-1、0.14μmol·L-1(ii) a The pH value of the nutrient solution is 5.5-6.5.
Preferably: the effective viable count in the bacterial manure is 5.0 multiplied by 109~8.0×109CFU/g。
The invention also provides application of the microbial fertilizer in crop planting.
Preferably: the crop is tomato.
Preferably: the plant height, stem thickness, root activity, overground part dry weight, underground part dry weight, total nitrogen in tomato roots, total nitrogen in tomato stems, total phosphorus in tomato roots, total phosphorus in tomato stems, total potassium in tomato leaves, quick-acting nitrogen in a matrix, sucrase activity in the matrix, alkaline phosphatase activity in the matrix, catalase activity in the matrix, the number of actinomycetes in the matrix, vitamin C in tomato fruits, soluble protein in tomato fruits, soluble solid matters in tomato fruits, lycopene in tomato fruits and the total amount of volatile substances are improved.
According to the technical scheme, compared with the prior art, the invention discloses and provides the microbial fertilizer and the application thereof, and compared with the prior art, the microbial fertilizer has obvious influence on the growth, yield and quality of tomatoes, the activity of matrixase and the number of microorganisms, and is an optimized fertilizer application formula suitable for the quality cultivation of the fresh-eating tomatoes in matrix cultivation.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a schematic diagram showing the effect of the added microbial fertilizer on the plant height of tomato.
FIG. 2 is a schematic diagram showing the influence of the added microbial fertilizer on the stem thickness of a tomato.
FIG. 3 is a schematic diagram showing the influence of the added microbial fertilizer on the activity of tomato roots.
FIG. 4 is a schematic diagram showing the effect of the added microbial fertilizer on the dry weight of the overground part of the tomato provided by the invention.
FIG. 5 is a schematic diagram showing the effect of the added microbial fertilizer on the dry weight of the underground part of a tomato.
FIG. 6 is a schematic diagram showing the effect of the added microbial fertilizer on the total nitrogen content in the roots, stems and leaves of tomato.
FIG. 7 is a schematic diagram showing the effect of the added microbial fertilizer on the total phosphorus content in the roots, stems and leaves of tomato.
FIG. 8 is a schematic diagram showing the effect of the added microbial fertilizer on the total potassium content in the roots, stems and leaves of tomato.
FIG. 9 is a schematic diagram of the influence of the added microbial fertilizer on the pH of the substrate.
FIG. 10 is a schematic diagram of the effect of adding microbial fertilizer on the conductivity of a substrate according to the present invention.
FIG. 11 is a schematic diagram showing the influence of the added microbial fertilizer on the content of the quick-acting nitrogen in the matrix.
FIG. 12 is a schematic diagram showing the influence of the added microbial fertilizer on the content of the fast-acting phosphorus in the matrix.
FIG. 13 is a schematic diagram showing the effect of the added microbial fertilizer on the content of the quick-acting potassium in the substrate.
FIG. 14 is a schematic diagram showing the effect of the added microbial fertilizer on the activity of sucrase enzyme in the substrate provided by the present invention.
FIG. 15 is a schematic diagram showing the effect of added microbial fertilizer on the activity of matrix urease enzyme.
FIG. 16 is a schematic diagram showing the effect of the added microbial fertilizer on the activity of alkaline phosphatase in the substrate.
FIG. 17 is a schematic diagram of the effect of added microbial fertilizer on substrate catalase enzyme activity according to the present invention.
FIG. 18 is a schematic diagram of the effect of the added microbial fertilizer on the number of bacteria in the substrate.
FIG. 19 is a schematic diagram showing the influence of the added microbial fertilizer on the number of actinomycetes in the substrate.
FIG. 20 is a schematic diagram illustrating the effect of microbial fertilizer addition on the number of fungi in a substrate according to the present invention.
FIG. 21 is a schematic diagram showing the effect of the microbial fertilizer on tomato yield.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the invention discloses a microbial fertilizer and application thereof.
In the embodiment of the invention, the microbial fertilizer comprises: 7 parts of Brevibacillus laterosporus AMCC 101583 with the preservation number of CGMCC No. 24137; 2 parts of Bacillus subtilis AMCC 100001 with the preservation number of CGMCC No. 6764; 1 part of Bacillus mucilaginosus (Bacillus mucoginosus) AMCC 100035 with the preservation number of CGMCC No.6766 and nutrient solution.
Example 1
The 1 tested fresh-eating tomato variety is 'Liao Feng 185', Kazaki tomato formula (1978) nutrient solution (Guo Shi Rong Master edition, second edition of soilless culture science) required raw materials purchased from Shanghai Yongtong chemical Co. Yeast cream was purchased from texas woudy microbial resources, llc.
Design of experiments
The test was carried out in the Minmura base of Jinan Lu Qing Miao GmbH in 7-2021, 2020. A commercial Luqing commodity matrix is used as a culture matrix, large and small rows are adopted for field planting, the large row spacing is 110cm, the small row spacing is 50cm, and the plant spacing is 33 cm. And (5) supplying water and fertilizer in a nutrient solution irrigation mode, and beginning to fertilize 30 days after planting.
The test was conducted with 3 treatments, using Kawasaki tomato formula as Control (CK), and J1 treatment was conducted by adding 187.5kg 667m to Kawasaki tomato formula-2(i.e. diluted 500 times) yeast extract, and the J2 treatment is prepared by adding 187.5L 667m onto Kawasaki tomato formula-2(i.e., diluted 500-fold) microbial inoculum. By researching the influence of different microbial fertilizers on the growth, yield and quality of the tomatoes and the activity and number of the matrixase, an optimized fertilizer formula suitable for the quality cultivation of the matrix-cultivated fresh-eating tomatoes is screened out.
2 test effects
2.1 Effect of microbial Fertilizer on tomato growth
2.1.1 Effect on plant height and Stem thickness
As can be seen from FIGS. 1 and 2, the plant height and stem thickness of the tomatoes treated by each treatment gradually increase with the increase of the growth period. The plant height after planting is 30-60 d, the growth rate is fastest, the difference between treatments is increased after 60-90 d, and the treatments J1 and J2 are obviously higher than CK at 90d after planting, and are respectively 6.5% higher and 7.6% higher. The stem thickness of each treated tomato is remarkably different at 60 days after permanent planting, and the treatment rates of J1 and J2 are respectively 11.4 percent and 13.5 percent higher than CK, so that the difference is remarkable.
2.1.2 Effect on root vigour
As can be seen from FIG. 3, the tomato root activity is stronger when the tomato is treated by adding the microbial fertilizer, and is higher when the tomato is treated by J2, J1 and J2 are both significantly higher than CK treatment, which are respectively 10.1% higher and 13.7% higher, but there is no significant difference between J1 and J2 treatment.
2.1.3 Effect on the Dry weight of the above and underground portions
As can be seen from fig. 4 and 5, the dry weight of the aerial parts of the tomato plants treated with the microbial fertilizer is significantly increased compared to CK, and is greater under the treatment of J2, and J1 and J2 are increased by 43.6% and 54.6%, respectively, but the difference between the treatments of J2 and J1 is not significant. The tomato underground dry weight was also higher under the J2 treatment, increased by 5.2% and 7.8% over the J1 and CK treatments, respectively, and was significantly higher than CK, but was not significantly different from the J1 treatment, and was also not significantly different from CK between the J1 treatment.
2.1.4 Effect on Total Nitrogen, Total phosphorus and Total Potassium content in tomato plants
As can be seen from fig. 6, the total nitrogen content in tomato roots is highest with J2 treatment, significantly higher than with CK, J1 treatment, followed by J1 treatment, with the lowest CK treatment content and no significant difference between CK and J1 treatments; the total nitrogen content in the stem was highest with J2 treatment, significantly higher than with J1 and CK treatment, and the difference between J1 and CK treatment was insignificant. The total nitrogen content in the leaves was higher with the J1 treatment, and the differences between treatments were not significant.
As can be seen from fig. 7, the total phosphorus content in tomato roots was highest with treatment of J2, with no significant difference from J1, with J1 and J2 being 8.8% and 10.9% higher than CK, respectively, with significant difference; the total phosphorus content in the stem is the highest with J2 treatment, and is obviously different from CK and J1 treatment, and J1 is not obviously different from CK. The total phosphorus content in the leaves is higher under the treatment of J1, the difference with the J2 treatment is not obvious, and the total phosphorus content in the leaves is 31.0 percent and 30.5 percent higher than that of CK respectively under the treatment of J1 and J2, and the difference is obvious.
As can be seen in fig. 8, the total potassium content in tomato roots was highest with J1 treatment, significantly higher than with CK and J2 treatments, with J2 being not significantly different from CK treatment. The total potassium content in the stem is the highest under the treatment of J2, which is 9.0 percent higher than that of CK treatment, and the difference is obvious; j1 treatment was the lowest, significantly lower than CK. The total potassium content in leaves was highest with J2 treatment, significantly higher by 23.9% and 20.0% compared to CK and J1, with significant differences.
2.2 Effect of microbial manure addition on the chemical Properties of the substrate
2.2.1pH and conductivity changes
As shown in fig. 9 and 10, the pH of the substrate tended to increase with the growth period, but the magnitude of the change was not significant, and the pH was nearly neutral for each treatment. The conductivity of the fertilizer shows a trend of increasing firstly and then decreasing as the growth period is prolonged, and the conductivity of the fertilizer is higher in each period of J1 and J2 than that of CK treatment, and the difference is obvious.
2.2.2 content Change of matrix available Nitrogen, available phosphorus and available Potassium
As can be seen from fig. 11, 12 and 13, the content of the available nitrogen in the substrate was higher with J2 treatment, which is 10.6% higher than that of CK treatment, and the difference was significant. The content of the quick-acting phosphorus is higher under the treatment of J1, is obviously higher than that of CK by 13.5 percent and is 5.4 percent higher than that of J2, and the difference is not obvious. The quick-acting potassium content is higher under the treatment of J1, and is treated by J2, wherein the treatment of J1 and J2 are respectively 72.5 percent and 43.2 percent higher than CK, and the difference is obvious.
2.2.3 Effect on matrine Activity
As can be seen from fig. 14, 15, 16 and 17, the sucrase activity in the matrix was higher in the treatment with J2, which is 22.0% and 20.5% higher than in the treatment with CK and J1, respectively, and the difference was significant. Urease activity is higher under J1 treatment, and J1 and J2 treatment have obvious difference with CK; the alkaline phosphatase activity is higher under the treatment of J2, and has no obvious difference with CK treatment, which is 66.4% higher than that of J1, and has obvious difference; the catalase activity is higher under the treatment of J2, is 12.1 percent and 16.8 percent higher than that of CK and J1, and has obvious difference.
2.2.4 Effect on the number of substrate microorganisms
As can be seen from fig. 18, 19 and 20, the number of bacteria in the substrate was the highest with the treatment of J2, which is 150.2% higher than that of CK treatment, and the difference was significant; the second is the treatment of J1, which is 108.3% higher than the CK treatment, and the difference is significant. The number of actinomycetes in the matrix is higher under the treatment of J2, then J1 treatment is carried out, and the treatment of J1 and J2 are respectively 31.2 percent and 37.6 percent higher than CK, and the difference is obvious. The number of fungi in the substrate was highest with CK treatment, 87.1% and 63.8% higher than with J1 and J2 treatments, with no significant difference between J1 and J2 treatments.
2.3 influence of microbial manure addition on tomato fruit quality
2.3.1 Effect on tomato fruit nutritional quality
As shown in table 1, the vitamin C content in the tomato fruits is highest under the treatment of J2, which is 70.0% higher than that of CK and 15.8% higher than that of J1, respectively, with significant difference; the next is the J1 process, with lower CK processing. Compared with CK, the soluble protein content of the tomatoes under the treatment of J1 and J2 is respectively 28.3% and 41.3%, and the difference is obvious. The soluble solid content in tomato fruits is higher under J2 treatment, and then J1 treatment, J1 and J2 are respectively 9.8 percent and 10.9 percent higher than CK treatment, and the difference is obvious. The lycopene content in the tomato fruits is higher under the treatment of J2, and then J1 treatment is carried out, wherein J1 and J2 are respectively 38.0 percent and 51.4 percent higher than CK, and the difference is obvious.
TABLE 1 Effect of microbial manure addition on tomato fruit quality
Figure RE-GDA0003543028820000071
2.3.2 Effect on volatile substance content
As can be seen from table 2, the volatile substances detected from all tomato fruits are aldehydes, alcohols and hydrocarbons in large amounts. The tomato fruits treated by J2 have the most detected volatile substances, and the total number of the volatile substances is 69, the total number of the volatile substances is 67 after CK treatment, and 59 of the volatile substances are in J1. The aldehydes detected under the treatment of J1 and J2 were 3 and 5 more than CK, respectively.
TABLE 2 influence of microbial fertilizer addition on the amount of various volatile substances in tomato fruit
Figure RE-GDA0003543028820000072
As can be seen from Table 3, the aldehyde substances in the tomato fruits are higher in hexanal content under the treatment of CK and J1, higher in 2-hexenal content under the treatment of J2, and higher in E-2-heptenal, E-2, 4-heptaenal, nonanal, E-2-nonanal and neral content under the treatment of J1 and J2 than CK; 4-methyl-hexanal and heptanal are substances peculiar to J1 treatment; 3-methyl butyraldehyde, E-2, 4-dodecadienal, 2-hexenal, 7-methyl-3-methylene-6-octenal are the special substances for T2 treatment.
The ketone substances in the tomato fruits treated by CK have higher content of 1-pentene-3-ketone and are 6-methyl-5-heptene-2-ketone; the content of 3-nonanone is higher in the treatment of J1, wherein 2,2, 3-trimethylcyclobutanone-is a specific substance of the treatment of J1; the J2 treatment has high content of 6-methyl-5-hepten-2-one, and 1-octen-3-one and D-verbenazone are the substances special for J2 treatment. The content of alpha-ionone and E-6, 10-dimethyl-5, 9-undecadienyl-2-ketone under the treatment of J1 and J2 is obviously higher than that of CK, and the content is higher under the treatment of J1.
Alcohol substances in tomato fruits treated by CK have higher contents of Z-2-pentene-1-alcohol and 6-methyl-5-hepten-2-ol, 3-nonanol in J1 treatment has higher content, 6-methyl-5-hepten-2-ol in J2 treatment has higher content, and n-pentanol, 3-methyl-1-butanol, cyclohexanol, 3, 5-dimethylcyclohexanol, cedrol and 2, 7-dimethyl-4, 5-octanediol are special substances treated by J2.
The esters in tomato fruits are high in content of 2-oxomethyl hexanoate, 2-methyl-1-butanol acetate, methyl hexanoate, 2-oxomethyl hexanoate, 2, 4-trimethyl-1, 3-pentanediol diisobutyrate and 3,5, 5-trimethyl isoamyl hexanoate are common substances in the treatment room, 4- (ethoxy) -2-oxobutyl-3-ethyl enoate and 12-tridecanoic acid methyl ester are specific substances under J1 treatment, and vinyl hexanoate, methyl undecanoate and cyclopropyl methanol acetate are specific substances under J2.
The hydrocarbon substances in the tomato fruits treated by CK have higher content of octamethylcyclotetrasiloxane, the hydrocarbon substances in the tomato fruits treated by J1 have higher content of pentamethylcyclopropane, the hydrocarbon substances in the tomato fruits treated by J2 have higher content of 3,7, 7-trimethyl-1, 3, 5-cycloheptatriene, 1, 3-pentadiene, 2-methyl-6-methylene-2-octene and cis-1-methyl-9-oxazacyclo [6.1.0] nonane are common substances of the treatments, 3,7, 7-trimethyl-1, 3, 5-cycloheptatriene, 5, 6-diethyl-cyclohexa-1, 3-diene, 1, 3-dimethyl-1-cyclohexene, methylpentene, 3, 7-dimethyl-3, 6-octadecadiene and 2-methyl-6-propyl-dodecane are unique substances under J2 treatment.
The other substances CK and J1 in tomato fruits are all high in 2-ethyl-furan content, J2 is high in 2-pentyl-furan content, and 2-methylfuran, 2-pentyl-furan and E-4-decenal are common substances treated.
TABLE 3 Effect of microbial Fertilizer addition on tomato fruit volatiles
Figure RE-GDA0003543028820000081
Figure RE-GDA0003543028820000091
Figure RE-GDA0003543028820000101
Figure RE-GDA0003543028820000111
2.4 Effect of microbial Fertilizer on tomato yield
As can be seen from fig. 21, tomato yields J1 and J2 treatments were significantly higher than CK, 24.1% and 31.0% higher than CK, respectively; but the differences between the J1 and J2 treatments were not significant, and the overall yield was represented by J2> J1> CK.
2.5 cultivation cost input and economic benefit analysis
As shown in Table 4, the cost part is removed under the treatment of adding different microbial fertilizers, the yield and the benefit of the treatment with J2 are higher, and the benefit is respectively 26.2% and 34.3% higher than that of CK treatment after J1 treatment.
Table 4 analysis of economic benefits under treatments (667 m)2)
Figure RE-GDA0003543028820000112
Figure RE-GDA0003543028820000121
Injecting; 7 yuan per kg of ammonium nitrate, 12 yuan per kg of monopotassium phosphate, 9 yuan per kg of monoammonium phosphate, 9 yuan per kg of potassium nitrate, 6.5 yuan per kg of calcium nitrate and 2.5 yuan per kg of magnesium sulfate; the tomato price is calculated according to 8 yuan/kg.
To sum up:
the invention has obvious influence on the growth, the yield and the quality of the tomatoes, the activity of matrixase and the number of microorganisms, and is an optimized fertilization formula suitable for the quality cultivation of the fresh-eating tomatoes in the matrix.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (6)

1. A microbial fertilizer, comprising: : 7 parts of Brevibacillus laterosporus AMCC 101583 with the preservation number of CGMCC No. 24137; 2 parts of Bacillus subtilis AMCC 100001 with the preservation number of CGMCC No. 6764; 1 part of Bacillus mucilaginosus (Bacillus mucoginosus) AMCC 100035 with the preservation number of CGMCC No.6766 and nutrient solution.
2. The microbial fertilizer according to claim 1, wherein the nutrient solution comprises: macroelements of nitrogen, phosphorus, potassium, calcium, magnesium and sulfur with concentrations of 7.7 mmol.L-1、0.7mmol·L-1、4.0mmol·L-1、1.5mmol·L-1、1.0mmol·L-1And 1.0 mmol. L-1(ii) a The trace elements of iron, manganese, copper, zinc, boron and molybdenum have the concentrations of 54 mu mol.L respectively-1、10μmol·L-1、0.32μmol·L-1、0.77μmol·L-1、46.3μmol·L-1、0.14μmol·L-1(ii) a The pH value of the nutrient solution is 5.5-6.5.
3. The microbial fertilizer according to claim 1 or 2, wherein the effective viable count of the microbial fertilizer is 5.0 x 109~8.0×109CFU/g。
4. Use of a microbial fertilizer according to any one of claims 1 to 3 in crop cultivation.
5. Use according to claim 4, wherein the crop is tomato.
6. The use of claim 5, wherein the plant height, stem thickness, root activity, dry weight of aerial parts, dry weight of underground parts, total nitrogen in tomato roots, total nitrogen in tomato stems, total phosphorus in tomato roots, total phosphorus in tomato stems, total potassium in tomato leaves, quick-acting nitrogen in a substrate, sucrase activity in a substrate, alkaline phosphatase activity in a substrate, catalase activity in a substrate, the number of actinomycetes in a substrate, vitamin C in tomato fruits, soluble protein in tomato fruits, soluble solids in tomato fruits, lycopene in tomato fruits, total amount of volatile substances are increased.
CN202210122147.2A 2022-02-09 2022-02-09 Microbial fertilizer and application thereof Pending CN114409456A (en)

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Publication number Priority date Publication date Assignee Title
CN106064974A (en) * 2016-06-06 2016-11-02 烟台中惠化肥有限公司 A kind of Water soluble fertilizer containing biological bacteria and preparation technology thereof
CN106116892A (en) * 2016-06-30 2016-11-16 兰赫(上海)生物科技有限公司 A kind of compound fertilizer producing green fruit-vegetable product and preparation method thereof
CN107118024A (en) * 2017-05-25 2017-09-01 湖北猛之牛农业科技有限公司 A kind of paddy rice-dedicated fertilizer
CN107986882A (en) * 2017-12-25 2018-05-04 陈益德 A kind of energy conservation and environmental protection organic suspension liquid compound fertilizer and preparation method thereof
CN111995458A (en) * 2020-07-23 2020-11-27 北京双龙阿姆斯科技有限公司 Nutrition absorption promoter for activating trunk epidermal cells

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106064974A (en) * 2016-06-06 2016-11-02 烟台中惠化肥有限公司 A kind of Water soluble fertilizer containing biological bacteria and preparation technology thereof
CN106116892A (en) * 2016-06-30 2016-11-16 兰赫(上海)生物科技有限公司 A kind of compound fertilizer producing green fruit-vegetable product and preparation method thereof
CN107118024A (en) * 2017-05-25 2017-09-01 湖北猛之牛农业科技有限公司 A kind of paddy rice-dedicated fertilizer
CN107986882A (en) * 2017-12-25 2018-05-04 陈益德 A kind of energy conservation and environmental protection organic suspension liquid compound fertilizer and preparation method thereof
CN111995458A (en) * 2020-07-23 2020-11-27 北京双龙阿姆斯科技有限公司 Nutrition absorption promoter for activating trunk epidermal cells

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