CN111939086A

CN111939086A - Eye mask with moisturizing and blackening functions and preparation method thereof

Info

Publication number: CN111939086A
Application number: CN202010859560.8A
Authority: CN
Inventors: 钟达金
Original assignee: Boya Guangzhou Biotechnology Research Institute Co ltd
Current assignee: Boya Guangzhou Biotechnology Research Institute Co ltd
Priority date: 2020-08-24
Filing date: 2020-08-24
Publication date: 2020-11-17

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to an eye mask with the effects of moisturizing and blackening and a preparation method thereof, wherein the eye mask comprises an eye mask base material and an eye mask essence, and the eye mask essence comprises the following components in parts by mass: the feed additive comprises 1-15% of Xylaria nigripes fermentation product extract, 1-3% of 1, 3-propylene glycol, 70.3-0.6% of palmitoyl tetrapeptide, 0.1-1% of hyaluronic acid, 0.01-0.03% of disodium ethylene diamine tetraacetate, 1-3% of hydroxyethyl cellulose and the balance of deionized water. The invention obtains a multi-effect Xylaria nigripes fermentation product extract by improving the Xylaria nigripes fermentation substrate, has excellent melanin eliminating capacity, has the functions of promoting eye microcirculation, protecting and reinforcing eye capillaries, smoothing fine wrinkles and moisturizing, has no irritation to skin, and can be used as a product for removing pigmented black eye circles and vascular black eye circles.

Description

Eye mask with moisturizing and blackening functions and preparation method thereof

Technical Field

The invention belongs to the technical field of cosmetics. More particularly, relates to an eye mask with the functions of moisturizing and removing black and a preparation method thereof.

Background

Eye skin thickness is very thin, is 1/3 ~ 1/5 of facial skin thickness only, and only sebaceous gland and metamorphosis sweat gland around the eye, and is little to eye skin protection, and eye skin fat content is few simultaneously, lacks elastic fiber and collagen structure, and the nerve fiber and the capillary distribution density around the eye are high, and blood circulation is not smooth etc. lead to the eye dim, and is tired, and then life and rest are irregular, and improper the eye will appear black eye circle, pouch, crow's feet, fat grain scheduling problem easily.

Among them, the dark eye circles are located in the ring shape under the two side orbits, and appear dark black or dark tea, which is a common eye problem. The black eye is classified into a plain black eye, a vascular black eye, a structural black eye and a mixed black eye in which the black eyes are combined at the same time, according to the cause. The reason of the pigmentary black eye is that melanin is locally accumulated around the eyes due to long-term sunlight irradiation, lack of nutrient substances, smoking, aging and the like, and the existence of a large amount of melanin particles is analyzed by detecting the corium layer of the black eye around the eyes; the blood vessel black eye is mainly caused by factors such as insufficient sleep, eye fatigue and the like, the blood flow in the capillary vessel is slow, the blood volume is increased, the oxygen consumption is increased, the hypoxia heme is greatly increased, and the phenomena of blood stasis and edema of the skin around the eyes are caused, and the blood vessel black eye appears after light reflection. At present, most people present mixed black eyes combined by a plurality of black eyes, such as pigmentary combined vascular black eyes, and most of the existing products are directed at the pigmentary black eyes and cannot effectively improve two types of black eyes at the same time.

Based on this, there is a need for an ophthalmic product that can target pigmented and vascular black eyes.

Disclosure of Invention

The invention aims to overcome the defects of the existing eye product and provide an eye mask with the effects of moisturizing and removing black, wherein the eye mask is added with a specially-made functional component Xylaria nigripes fermentation product extract, so that the eye mask can obviously promote the microcirculation of eye skin, protect and reinforce capillary vessels, smooth fine wrinkles and has double black eye circle removing effects.

The above purpose of the invention is realized by the following technical scheme: the eye mask comprises an eye mask base material and an eye mask essence, wherein the eye mask essence comprises the following components in percentage by mass: the feed additive comprises 1-15% of Xylaria nigripes fermentation product extract, 1-3% of 1, 3-propylene glycol, 70.3-0.6% of palmitoyl tetrapeptide, 0.1-1% of hyaluronic acid, 0.01-0.03% of disodium ethylene diamine tetraacetate, 1-3% of hydroxyethyl cellulose and the balance of deionized water.

Further, the eye mask essence comprises the following components in percentage by mass: 12% of Xylaria nigripes fermentation product extract, 3% of 1, 3-propylene glycol, 70.5% of palmitoyl tetrapeptide, 0.5% of hyaluronic acid, 0.02% of disodium ethylene diamine tetraacetate, 1.5% of hydroxyethyl cellulose and the balance of deionized water.

Further, the eye mask base material is prepared from one of bamboo charcoal fiber, silk fiber, seaweed fiber or cotton fiber. Furthermore, the silk eye mask base material is preferably prepared from silk fibers.

Further, the Xylaria nigripes fermentation product extract is prepared by taking 1-12% by weight of lactobacillus/soybean composite explant fermentation product, 0.1-2% by weight of glucose, 0.5-1% by weight of magnesium sulfate, 0.1-0.5% by weight of citric acid and the balance of deionized water as fermentation substrates, and inoculating 10-15% of Xylaria nigripes for fermentation.

Further, the fermentation substrate consists of: 5-12% of lactobacillus/soybean composite explant fermentation product, 1-2% of glucose, 0.5-0.8% of magnesium sulfate, 0.2-0.5% of citric acid and the balance of deionized water.

Further, the liquid state fermentation process parameters are as follows: the pH value is 5.3-5.6; the culture temperature is 22-25 ℃; the culture time is 6-8 days; the stirring speed is 100-200 rpm; the ventilation volume is 1-4 vvm; the pot pressure is 0.02-0.06 MPa.

Further, the liquid fermentation also comprises the steps of extracting the fermentation filtrate by adopting a solvent, filtering, freezing and drying to obtain the Xylaria nigripes fermentation product extract.

Further, the solvent is one or two of water or an organic solvent. Preferably, ethanol solution with volume fraction of 80-95% is adopted.

Further, the preparation method of the lactobacillus/soybean compound explant fermentation product comprises the following steps:

s1, cleaning the soybean compound explant, drying, adding distilled water, pulping, filtering pulp, and sterilizing to obtain a culture solution;

s2, inoculating the lactobacillus subjected to secondary culture into the culture solution according to the inoculation amount of 5-10%, adding 0.5-2% of glucose, fermenting at 37 ℃ for 1-3 days, after the fermentation is finished, freeze-drying the fermentation product, and grinding to obtain the lactobacillus fermented product; in the fermentation process: the tank pressure is 0.01-0.03 MPa; the ventilation volume is 1-3 vvm; the rotation speed is 140-160 rpm.

Further, the preparation method of the soybean compound explant comprises the following steps: cleaning and sterilizing soybean seeds, culturing and accelerating germination in a germination culture medium by illumination, and removing seed coats from seed eyes after accelerating germination to obtain the soybean compound explant.

Research has pointed out that the metabolic products secreted by Xylaria nigripes (KI) Sacc contain compounds with antioxidant function, so the fermentation products of Xylaria nigripes obtained by liquid culture fermentation have been disclosed to be used as whitening and moisturizing extracts, but the metabolic products obtained by different culture modes have different inevitable differences and have different efficacy levels, and even new application and efficacy are possible. In the meantime, fermentation of Xylaria nigripes using the "Lactobacillus/Glycine max complex explant fermentation product" herein as the main fermentation substrate material was not found.

The invention is characterized in that a lactobacillus/soybean compound explant fermentation product is used as a main fermentation substrate for liquid fermentation of Xylaria nigripes to obtain an extract of the Xylaria nigripes fermentation product. Tests show that compared with the traditional culture medium, the Xylaria nigripes fermentation product extract obtained by adopting the special fermentation substrate has more excellent melanin eliminating capability, has the effects of promoting eye microcirculation, protecting and reinforcing eye capillaries, smoothing fine wrinkles and moisturizing, and has no irritation to skin, so that the Xylaria nigripes fermentation product extract can be used as a product for eliminating pigmented black eye circles and vascular black eye circles.

The invention also aims to provide a method for preparing the eye mask with the effects of moisturizing and blackening, which comprises the following steps:

A) adding deionized water and disodium ethylene diamine tetraacetate into a stirring pot, and uniformly stirring;

B) dispersing hydroxyethyl cellulose and hyaluronic acid in 1, 3-propylene glycol, putting into a stirring pot, heating to 80-85 ℃, and homogenizing for 1-5 min; cooling to 40-45 ℃, adding the Xylaria nigripes fermentation product extract and palmitoyl tetrapeptide-7, and uniformly stirring; cooling to 30-35 ℃, discharging after the inspection is qualified, and filling the eye mask essence into an eye mask bag which is filled with an eye mask base material in advance.

The invention has the following beneficial effects:

the Xylaria nigripes fermentation product extract with multiple functions is obtained by improving the Xylaria nigripes fermentation substrate, has excellent melanin eliminating capacity, has the effects of promoting eye microcirculation, protecting and reinforcing eye capillaries, smoothing fine wrinkles and moisturizing, has no irritation to skin, and can be used as a product for removing pigmented black eye circles and vascular black eye circles.

Detailed Description

The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.

Unless otherwise indicated, reagents and materials used in the following examples are commercially available.

Example 1 preparation of Soybean Complex explants

The preparation method of the soybean compound explant comprises the following steps: cleaning soybean seeds, sterilizing, culturing in germination medium under illumination for accelerating germination, and removing seed coat from eyes of the seeds.

The germination medium comprises the following components: 20g/L glucose and agar with the mass percent concentration of 0.8 percent, and the pH value is 5.6.

Example 2 preparation of Lactobacillus/Soybean Complex explant fermentation product

S1, cleaning the soybean compound explant prepared in the example 1, drying, adding distilled water, pulping, filtering to remove residues, and sterilizing the pulp at 121 ℃ to obtain a culture solution;

s2, mixing the lactobacillus plantarum with sterilized cow milk, culturing for 24h at 36 ℃, taking 1ml of the cultured cow milk mixed solution to inoculate MRS broth culture medium, and culturing for 48 h;

s3, inoculating lactobacillus subjected to secondary culture into the culture solution according to the inoculation amount of 10%, adding 1% of glucose, fermenting at 37 ℃ for 3 days, filtering a fermentation product after fermentation is finished, freeze-drying, and grinding to obtain the lactobacillus fermented product; in the fermentation process: the tank pressure is 0.01-0.03 MPa; the ventilation volume is 1-3 vvm; the rotation speed was 140 rpm.

Example 3 preparation of extract of Xylaria nigripes fermentation product

Step one, inoculating Xylaria nigripes into a seeding tank according to the inoculation amount of 6%, and culturing for 4 days under the conditions that the pH is 5.0-5.5 and the temperature is 25 ℃; the seed culture medium comprises the following components: per 1000ml of water contained: 30g of glucose, 2g of ammonium sulfate, 1g of dipotassium phosphate trihydrate, 3g of glutamic acid, 3g of triammonium citrate and 3mg of zinc sulfate;

step two, transferring the culture solution of the Xylaria nigripes seeds in the step one to fermentation containing a fermentation substrate according to 10 v/v%, culturing for 6 days under the conditions that the pH value is 5.3-5.6 and the culture temperature is 25 ℃, and stopping fermentation after the fermentation substrate is filled with hyphae; the stirring speed is 100-200 rpm; the ventilation volume is 1-4 vvm; the tank pressure is 0.02-0.06 MPa;

and step three, taking the fermentation filtrate, concentrating, standing for 12 hours, adding ethanol solution with the volume fraction of 85% which is 1.2 times of the fermentation filtrate under the stirring state, standing for complete precipitation, taking the supernatant, and performing the steps of sterilization, ultrafiltration membrane filtration, freeze drying and porphyrization to obtain the Xylaria nigripes fermentation product extract.

The fermentation substrate comprises the following components: 10 percent of lactobacillus/soybean compound explant fermentation product, 1.5 percent of glucose, 0.6 percent of magnesium sulfate, 0.4 percent of citric acid and the balance of deionized water.

Example 4 preparation of extract of Xylaria nigripes fermentation product

step two, transferring the culture solution of the Xylaria nigripes seeds in the step one to fermentation containing a fermentation substrate according to 12 v/v%, culturing for 8 days under the conditions that the pH value is 5.3-5.6 and the culture temperature is 25 ℃, and stopping fermentation after the fermentation substrate is filled with hyphae; the stirring speed is 100-200 rpm; the ventilation volume is 1-4 vvm; the tank pressure is 0.02-0.06 MPa;

and step three, taking the fermentation filtrate, concentrating, standing for 12 hours, adding an ethanol solution with the volume fraction of 90% which is 2 times that of the fermentation filtrate under the stirring state, standing for complete precipitation, taking the supernatant, and performing the steps of sterilization, ultrafiltration membrane filtration, freeze drying and grinding to obtain the Xylaria nigripes fermentation product extract.

The fermentation substrate comprises the following components: 8 percent of lactobacillus/soybean compound explant fermentation product, 1.0 percent of glucose, 0.5 percent of magnesium sulfate, 0.4 percent of citric acid and the balance of deionized water.

Example 5 preparation of extract of Xylaria nigripes fermentation product

step two, transferring the culture solution of the Xylaria nigripes seeds in the step one to fermentation containing a fermentation substrate according to 15 v/v%, culturing for 6 days under the conditions that the pH value is 5.3-5.6 and the culture temperature is 25 ℃, and stopping fermentation after the fermentation substrate is filled with hyphae; the stirring speed is 100-200 rpm; the ventilation volume is 1-4 vvm; the tank pressure is 0.02-0.06 MPa;

and step three, taking the fermentation filtrate, concentrating, standing for 12 hours, adding ethanol solution with the volume fraction of 92% which is 1.5 times of the fermentation filtrate under the stirring state, standing for complete precipitation, taking the supernatant, and performing the steps of sterilization, ultrafiltration membrane filtration, freeze drying and porphyrization to obtain the Xylaria nigripes fermentation product extract.

The fermentation substrate comprises the following components: 12 percent of lactobacillus/soybean compound explant fermentation product, 2 percent of glucose, 0.8 percent of magnesium sulfate, 0.5 percent of citric acid and the balance of deionized water.

Examples 6 to 8 eye mask essences were formulated and used in amounts (% by mass)

The preparation method comprises the following steps:

B) dispersing hydroxyethyl cellulose and hyaluronic acid in 1, 3-propylene glycol, adding into a stirring pot, heating to 80 deg.C, and homogenizing for 5 min; cooling to 40 deg.C, adding Xylaria nigripes fermented product extract and palmitoyl tetrapeptide-7, and stirring; cooling to 35 deg.C, inspecting, discharging, and filling into eye mask bag containing eye mask base material.

Comparative example 1

The difference from example 6 is that the fermentation substrate used in the first step in the preparation of the extract of Xylaria nigripes fermentation product consists of: 10% by weight of lactobacillus/soy extract fermentation filtrate, 1.5% by weight of glucose, 0.6% by weight of magnesium sulfate, 0.4% by weight of citric acid and the balance deionized water.

Comparative example 2

The difference from example 6 is that the fermentation substrate used in the first step in the preparation of the extract of Xylaria nigripes fermentation product consists of: 1.5% by weight of glucose, 0.6% by weight of magnesium sulfate, 0.4% by weight of citric acid and the balance deionized water.

Comparative example 3

The difference from the example 6 is that the Xylaria nigripes extract prepared in the example 1 in the Chinese patent CN105411951B is adopted to replace the eye essence prepared by the Xylaria nigripes fermentation product extract.

Test one, skin irritation test

Referring to the reaction in the multiple skin irritation test method in the cosmetic hygiene code (2007 edition), the eye mask essence of examples 6 to 8 was subjected to multiple skin irritation tests, before the tests, the back hairs on both sides of the spinal column of the test animal were cut off, the hair removal range was 2cm × 2cm, the application area was 1.5 × 1.5cm, 2ml of the test sample was applied to one side, the control was applied to one side, the test sample was continuously applied for 14d every day, the hair was cut before each application from the 2d, the residual test sample was removed with warm water, and the results were observed after 1 h. The test results were scored according to the scoring criteria in the cosmetic hygiene code (2007 edition), the average score per animal per day was calculated, and the skin irritation intensity was judged according to table 1 below, and the test results are shown in table 1 below.

TABLE 1 stimulation intensity rating

Integral mean value	Strength of
		0～＜0.5	Has no irritation
0.5～＜2.0	Light irritation
		2.0～＜6.0	Middle irritation
6.0～8.0	Strong irritation

TABLE 2 results of multiple skin irritation tests

Sample (I)	Mean of daily score for each animal	Intensity of stimulus
			Example 6	0.10	Has no irritation
Example 7	0.13	Has no irritation
			Example 8	0.15	Has no irritation

Test II, removal test of pigmented black eye

1.1 subject inclusion and exclusion criteria: 70 patients with pigmented black eyes were selected, with the average age of 32 ± 1 year, and the inclusion criteria: self-evaluation of dark circles; secondly, the eye drops have obvious pigmentary black eye circles evaluated by clinicians, including simple pigmentary black eye circles and mixed black eye circles mainly based on the pigmentary property, and the VISIA facial imaging instrument is adopted to detect the visible obvious pigmentation of the upper eyelid and the lower eyelid. Exclusion criteria: women in pregnancy and lactation; ② people allergic to the components of the product; ③ patients with infection or inflammation around the eyes; fourthly, the scar is obvious around the eyes; fifthly, the patients who use externally applied medicines or similar products in 2 weeks; sixthly, the eye receives any cosmetic treatment in the last half year.

1.2 test methods: the 70 patients were randomly divided into 7 groups, namely, examples 6 to 8 and comparative examples 1 to 3 and a blank control group, and the subjects except the blank control group were externally applied with the product 1 time in the morning and evening per day, each time for 15min, and continuously used for 56 days, the skin melanin content MI values before use, and on the 28 th and 56 th days of use of the subjects were tested by a German Mexameter MX-18 erythema/melanin index tester, the measurement was repeated 3 times for each measurement, the average value was obtained, statistical difference analysis was performed, and the test results are shown in Table 3.

TABLE 3 skin melanin MI values

Note: compared with the traditional Chinese medicine preparation before use,^*P＜0.05；^**p is less than 0.01, and the larger the MI value of the skin melanin content is, the higher the skin melanin content is.

As can be seen from the above table, after 28 days using the eye repair essence of examples 6 to 8 of the present invention, the melanin content in the skin of the eyes significantly changed from that before use, and after 56 days using the eye repair essence of the present invention, the melanin content in the skin of the eyes significantly changed from that before use, indicating that the eye repair essence of the present invention has an excellent improvement effect on a pigmented black eye.

Comparative example 1 the eye essence obtained by using the filtrate of the lactobacillus/soybean extract fermentation product instead of the fermentation product of the lactobacillus/soybean composite explant of the invention has no obvious change in the content of melanin in the eyes before use on the 28 th day and has obvious change after the 56 th day; comparative example 2 functions similarly to comparative example 1; comparative example 3 the Xylaria nigripes extract prepared in example 1 of Chinese patent CN105411951B is used as an effective component to prepare the eye essence, and the eye essence has a good removing effect on pigment black eyes.

Experiment three, vascular black eye removal experiment

1.1 subject inclusion and exclusion criteria: 60 patients with pigmented black eyes were selected, with the average age of 30 ± 1 year, and the inclusion criteria were: self-evaluation of the red-eye health-care product has dark eye circles, and pink red (oxyhemoglobin) or bluish purple (hemoglobin) appears under sunlight; and secondly, the blood vessel black eye is obvious through evaluation of a clinician, and observation under a wood lamp and comparison under a fluorescent lamp have no obvious change. Exclusion criteria: women in pregnancy and lactation; ② people allergic to the components of the product; ③ patients with infection or inflammation around the eyes; fourthly, the scar is obvious around the eyes; fifthly, the patients who use externally applied medicines or similar products in 2 weeks; sixthly, the eye receives any cosmetic treatment in the last half year.

1.2 test methods: the above 60 patients were randomly divided into 6 groups, examples 6 to 8 and comparative examples 1 to 3, and the subjects were externally applied 1 time each day in the morning and evening for 15min for 56 days, and skin Erythema Indexes (EI) before and at 56 days were measured using a German Mexameter MX-18 erythema/melanin index tester, and the measurement was repeated 3 times for each measurement, and the average value was averaged to perform statistical difference analysis, and the test results are shown in Table 4.

TABLE 4 skin erythema index test results

Note: compared with the traditional Chinese medicine preparation before use,^*P＜0.05；^**p is less than 0.01, and the larger the skin EI value is, the higher the skin heme content is.

As can be seen from the above table, after 56 days using the eye repair essence of examples 6 to 8 of the present invention, the value of erythema index of the skin on the eyes was significantly reduced compared to that before the use, which proves that it can be used for improving vascular black eye, while the indexes of erythema index of the rest groups were not significantly reduced compared to that before the use.

Fourth test, moisture retention test

60 subjects are selected, the age range is 28-40 years, the average age is 34 years, the subjects are randomly divided into 6 groups, the eye repair essence products of the invention in the examples 6-8 and the comparative examples 1-3 are externally applied to each group of subjects, eye skin moisture content of the subjects before and after use for 30min, 60min, 2h and 4h is measured by a Corneometer CM 825 skin moisture content tester, the skin moisture content increase rate is calculated according to the following formula, and the test results are shown in the following table 5.

In the formula, MMV₀MMV for pre-use skin; MMV_tIs the skin MMV t period after use.

TABLE 5 moisture content growth rate of eye skin

From the above table, the Xylaria nigripes fermentation product extract obtained by using the lactobacillus/soybean compound explant fermentation product of the invention as the main fermentation substrate for Xylaria nigripes liquid fermentation has more excellent moisturizing effect, the skin water content shows a gradually increasing trend, the peak value is reached 4h after the use, and the effects of instant moisturizing and long-acting moisturizing are achieved.

Test five, smoothing fine lines test

Selecting qualified 60 subjects with average age of 32 years, and applying the product to each group of subjects for 1 time in the morning and evening, each time for 15min, continuously using for 56 days, and cleaning the face with the facial cleanser before each use. The facial skin wrinkle data before and after day 56 of use were examined for each group of subjects using a VISA skin detector, test environment: the test results are shown in the following table 6, wherein the temperature is 20-22 ℃, and the humidity is 40-60%.

TABLE 6 wrinkle reduction test results

Sample (I)	Wrinkle characterization technique Change Rate (%)	Wrinkle score Change (%)
			Example 6	-18.68％	-12.13％
Example 7	-15.37％	-10.35％
			Example 8	-16.25％	-10.47％
Comparative example 1	-12.73％	-6.15％
			Comparative example 2	-9.28％	-4.39％
Comparative example 3	-10.41％	-5.60％

Note:

the above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims

1. The eye mask with the effects of moisturizing and blackening is characterized by comprising an eye mask base material and an eye mask essence, wherein the eye mask essence comprises the following components in percentage by mass: the feed additive comprises 1-15% of Xylaria nigripes fermentation product extract, 1-3% of 1, 3-propylene glycol, 70.3-0.6% of palmitoyl tetrapeptide, 0.1-1% of hyaluronic acid, 0.01-0.03% of disodium ethylene diamine tetraacetate, 1-3% of hydroxyethyl cellulose and the balance of deionized water.

2. The eye mask with the effects of moisturizing and blackening the skin according to claim 1, wherein the eye mask essence comprises the following components in percentage by mass: 12% of Xylaria nigripes fermentation product extract, 3% of 1, 3-propylene glycol, 70.5% of palmitoyl tetrapeptide, 0.5% of hyaluronic acid, 0.02% of disodium ethylene diamine tetraacetate, 1.5% of hydroxyethyl cellulose and the balance of deionized water.

3. The eye mask with the effects of moisturizing and blackening the skin according to claim 1, wherein the Xylaria nigripes fermentation product extract is prepared by taking 1-12% by weight of a lactobacillus/soybean composite explant fermentation product, 0.1-2% by weight of glucose, 0.5-1% by weight of magnesium sulfate, 0.1-0.5% by weight of citric acid and the balance of deionized water as a fermentation substrate, and inoculating 10-15% of Xylaria nigripes for fermentation.

4. The eye mask having moisturizing and blackening effects of claim 3, wherein the fermentation substrate comprises: 5-12% of lactobacillus/soybean composite explant fermentation product, 1-2% of glucose, 0.5-0.8% of magnesium sulfate, 0.2-0.5% of citric acid and the balance of deionized water.

5. The eye mask with the effects of moisturizing and blackening as claimed in claim 3 or 4, wherein the liquid fermentation process parameters are as follows: the pH value is 5.3-5.6; the culture temperature is 22-25 ℃; the culture time is 6-8 days; the stirring speed is 100-200 rpm; the ventilation volume is 1-4 vvm; the pot pressure is 0.02-0.06 MPa.

6. The eye mask having the effects of moisturizing and blackening, according to claim 5, wherein the liquid fermentation further comprises the steps of extracting the fermentation filtrate with a solvent, filtering, and freeze-drying to obtain an extract of a fermentation product of Xylaria nigripes.

7. The eye mask having the effects of moisturizing and blackening as claimed in claim 6, wherein the solvent is one or both of water and an organic solvent.

8. The eye mask with the effects of moisturizing and blackening hair according to claims 3 and 4, wherein the lactobacillus/soybean compound explant fermentation product is prepared by a method comprising the following steps:

9. The eye mask with the effects of moisturizing and blackening the skin according to claim 8, wherein the soybean compound explant is prepared by the following steps: cleaning and sterilizing soybean seeds, culturing and accelerating germination in a germination culture medium by illumination, and removing seed coats from seed eyes after accelerating germination to obtain the soybean compound explant.

10. A method for preparing the eye mask with the effects of moisturizing and blackening the skin as claimed in any one of claims 1 to 9, which is characterized by comprising the following steps: