CN111728936A

CN111728936A - Eye repair essence and preparation method thereof

Info

Publication number: CN111728936A
Application number: CN202010845211.0A
Authority: CN
Inventors: 钟达金
Original assignee: Boya Guangzhou Biotechnology Research Institute Co ltd
Current assignee: Boya Guangzhou Biotechnology Research Institute Co ltd
Priority date: 2020-08-20
Filing date: 2020-08-20
Publication date: 2020-10-02

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to an eye repair essence and a preparation method thereof, wherein the eye repair essence comprises the following components in percentage by mass: 1-4% of humectant, 0.1-0.2% of thickener, 1-6% of emollient oil, 0.5-2% of emulsifier, 1-12% of Xylaria nigripes fermentation product extract, 0.1-0.2% of preservative, 0.01-0.03% of chelating agent, 0.01-0.02% of essence and the balance of deionized water. The essence disclosed by the invention is added with the Xylaria nigripes fermentation product extract prepared by a special process, can effectively remove melanin in eyes, has the effects of promoting microcirculation of the eyes, protecting and reinforcing capillary vessels of the eyes, smoothing fine wrinkles and moisturizing, has no irritation to skin, and can be used as a product for removing pigmented black eye circles and vascular black eye circles.

Description

Eye repair essence and preparation method thereof

Technical Field

The invention belongs to the technical field of cosmetics. More particularly, relates to an eye repair essence and a preparation method thereof.

Background

Eye skin thickness is very thin, is 1/3 ~ 1/5 of facial skin thickness only, and only sebaceous gland and metamorphosis sweat gland around the eye, and is little to eye skin protection, and eye skin fat content is few simultaneously, lacks elastic fiber and collagen structure, and the nerve fiber and the capillary distribution density around the eye are high, and blood circulation is not smooth etc. lead to the eye dim, and is tired, and then life and rest are irregular, and improper the eye will appear black eye circle, pouch, crow's feet, fat grain scheduling problem easily.

Among them, the dark eye circles are located in the ring shape under the two side orbits, and appear dark black or dark tea, which is a common eye problem. The black eye is classified into a plain black eye, a vascular black eye, a structural black eye and a mixed black eye in which the black eyes are combined at the same time, according to the cause. The reason of the pigmentary black eye is that melanin is locally accumulated around the eyes due to long-term sunlight irradiation, lack of nutrient substances, smoking, aging and the like, and the existence of a large amount of melanin particles is analyzed by detecting the corium layer of the black eye around the eyes; the blood vessel black eye is mainly caused by factors such as insufficient sleep, eye fatigue and the like, the blood flow in the capillary vessel is slow, the blood volume is increased, the oxygen consumption is increased, the hypoxia heme is greatly increased, and the phenomena of blood stasis and edema of the skin around the eyes are caused, and the blood vessel black eye appears after light reflection. At present, most people present mixed black eyes combined by a plurality of black eyes, such as pigmentary combined vascular black eyes, and most of the existing products are directed at the pigmentary black eyes and cannot effectively improve two types of black eyes at the same time.

Based on this, there is a need for an ophthalmic product that can target pigmented and vascular black eyes.

Disclosure of Invention

The invention aims to overcome the defects of the existing eye repairing product, provides an eye product with double black eye circle removing effect, can remarkably promote the microcirculation of the skin of the eye, protect and reinforce the capillary vessels, smooth fine lines and has double black eye circle removing effect.

The above purpose of the invention is realized by the following technical scheme: the eye repair essence comprises the following components in percentage by mass: 1-4% of humectant, 0.1-0.2% of thickener, 1-6% of emollient oil, 0.5-2% of emulsifier, 1-12% of Xylaria nigripes fermentation product extract, 0.1-0.2% of preservative, 0.01-0.03% of chelating agent, 0.01-0.02% of essence and the balance of deionized water.

Further, the Xylaria nigripes fermentation product extract is prepared by taking 1-12% by weight of lactobacillus/soybean composite explant fermentation product, 0.1-2% by weight of glucose, 0.5-1% by weight of magnesium sulfate, 0.1-0.5% by weight of citric acid and the balance of deionized water as fermentation substrates, and inoculating 10-15% of Xylaria nigripes for fermentation.

Further, the fermentation substrate consists of: 5-12% of lactobacillus/soybean composite explant fermentation product, 1-2% of glucose, 0.5-0.8% of magnesium sulfate, 0.2-0.5% of citric acid and the balance of deionized water.

Further, the fermentation process parameters are as follows: the pH value is 5.3-5.6; the culture temperature is 22-25 ℃; the culture time is 6-8 days; the stirring speed is 100-200 rpm; the ventilation volume is 1-4 vvm; the pot pressure is 0.02-0.06 MPa.

Further comprises the steps of extracting the fermentation filtrate by adopting a solvent, filtering, freezing and drying to obtain the Xylaria nigripes fermentation product extract.

Further, the solvent is one or two of water or an organic solvent. Furthermore, the solvent is preferably alcohol, and preferably ethanol solution with the mass fraction of 80-95%.

Further, the moisturizer is selected from moisturizers commonly used in the cosmetic field, including but not limited to: sodium hyaluronate, allantoin, glycerol, 1, 2-butanediol or 1, 2-pentanediol.

Further, the thickener is selected from thickeners commonly used in the cosmetic field, including but not limited to: acrylic acid (ester) C10-30 alkanol acrylate cross-linked polymer, carbomer, xanthan gum or sodium alginate.

Further, the emollient oils are selected from emollient oils commonly used in the cosmetic field, including but not limited to: isononyl isononanoate, macadamia nut oil, caprylic/capric triglyceride, squalane.

Further, the emulsifier is selected from emulsifiers commonly used in the cosmetic field, including but not limited to: PEG-30-dipolyhydroxystearate and cetearyl glucoside.

Further, the preservative is phenoxyethanol and sorbic acid.

Further, the chelating agent is EDTA-2 Na.

Further, the preparation method of the lactobacillus/soybean compound explant fermentation product comprises the following steps:

s1, cleaning the soybean compound explant, drying, adding distilled water, pulping, filtering pulp, and sterilizing to obtain a culture solution;

s2, inoculating the lactobacillus subjected to secondary culture into the culture solution according to the inoculation amount of 5-10%, adding 0.5-2% of glucose, fermenting at 37 ℃ for 1-3 days, after the fermentation is finished, freeze-drying the fermentation product, and grinding to obtain the lactobacillus fermented product; in the fermentation process: the tank pressure is 0.01-0.03 MPa; the ventilation volume is 1-3 vvm; the rotation speed is 140-160 rpm.

Further, the preparation method of the soybean compound explant comprises the following steps: cleaning and sterilizing soybean seeds, culturing and accelerating germination in a germination culture medium by illumination, and removing seed coats from seed eyes after accelerating germination to obtain the soybean compound explant.

Another object of the present invention is to provide a method for preparing the eye-repairing essence, comprising the steps of:

A) putting deionized water, a humectant, a thickener and a chelating agent into a water phase pot, uniformly stirring, heating and stirring to 80-85 ℃, and preserving heat for 5-10 min;

B) putting an emulsifier and skin-moistening oil into an oil phase pot, heating to 80-85 ℃, and uniformly stirring;

C) filtering the materials in the water phase pot, pumping into an emulsifying pot, filtering the materials in the oil phase pot, slowly pumping into the emulsifying pot, uniformly stirring, and homogenizing for 3-6 min; cooling to 40 deg.C, adding Xylaria nigripes fermentation product extract, antiseptic and essence, stirring, and discharging after inspection.

Research has pointed out that the metabolic products secreted by Xylaria nigripes (KI) Sacc contain compounds with antioxidant function, so the fermentation products of Xylaria nigripes obtained by liquid culture fermentation have been disclosed to be used as whitening and moisturizing extracts, but the metabolic products obtained by different culture modes have different inevitable differences and have different efficacy levels, and even new application and efficacy are possible. In the meantime, fermentation of Xylaria nigripes using the "Lactobacillus/Glycine max complex explant fermentation product" herein as the main fermentation substrate material was not found.

The invention is characterized in that a lactobacillus/soybean compound explant fermentation product is used as a main fermentation substrate for liquid fermentation of Xylaria nigripes to obtain an extract of the Xylaria nigripes fermentation product. Tests show that compared with the traditional culture medium, the Xylaria nigripes fermentation product extract obtained by adopting the special fermentation substrate has more excellent melanin eliminating capability, has the effects of promoting eye microcirculation, protecting and reinforcing eye capillaries, smoothing fine wrinkles and moisturizing, and has no irritation to skin, so that the Xylaria nigripes fermentation product extract can be used as a product for eliminating pigmented black eye circles and vascular black eye circles.

The invention has the following beneficial effects:

the Xylaria nigripes fermentation product extract with multiple functions is obtained by improving the Xylaria nigripes fermentation substrate, has excellent melanin eliminating capacity, has the effects of promoting eye microcirculation, protecting and reinforcing eye capillaries, smoothing fine wrinkles and moisturizing, has no irritation to skin, and can be used as a product for removing pigmented black eye circles and vascular black eye circles.

Detailed Description

The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.

Unless otherwise indicated, reagents and materials used in the following examples are commercially available.

Lactobacillus plantarum, purchased from China center for agricultural microbial culture Collection.

Example 1 preparation of Soybean Complex explants

The preparation method of the soybean compound explant comprises the following steps: cleaning soybean seeds, sterilizing, culturing in germination medium under illumination for accelerating germination, and removing seed coat from eyes of the seeds.

The germination medium comprises the following components: 20g/L glucose and agar with the mass percent concentration of 0.8 percent, and the pH value is 5.6.

Example 2 preparation of Lactobacillus/Soybean Complex explant fermentation product

S1, cleaning the soybean compound explant prepared in the example 1, drying, adding distilled water, pulping, filtering to remove residues, and sterilizing the pulp at 121 ℃ to obtain a culture solution;

s2, mixing the lactobacillus plantarum with sterilized cow milk, culturing for 24h at 36 ℃, taking 1ml of the cultured cow milk mixed solution to inoculate MRS broth culture medium, and culturing for 48 h;

s3, inoculating lactobacillus subjected to secondary culture into the culture solution according to the inoculation amount of 10%, adding 1% of glucose, fermenting at 37 ℃ for 3 days, filtering a fermentation product after fermentation is finished, freeze-drying, and grinding to obtain the lactobacillus fermented product; in the fermentation process: the tank pressure is 0.01-0.03 MPa; the ventilation volume is 1-3 vvm; the rotation speed was 140 rpm.

Example 3 preparation of extract of Xylaria nigripes fermentation product

Step one, inoculating Xylaria nigripes into a seeding tank according to the inoculation amount of 6%, and culturing for 4 days under the conditions that the pH is 5.0-5.5 and the temperature is 25 ℃; the seed culture medium comprises the following components: per 1000ml of water contained: 30g of glucose, 2g of ammonium sulfate, 1g of dipotassium phosphate trihydrate, 3g of glutamic acid, 3g of triammonium citrate and 3mg of zinc sulfate;

step two, transferring the culture solution of the Xylaria nigripes seeds in the step one to fermentation containing a fermentation substrate according to 10 v/v%, culturing for 6 days under the conditions that the pH value is 5.3-5.6 and the culture temperature is 25 ℃, and stopping fermentation after the fermentation substrate is filled with hyphae; the stirring speed is 100-200 rpm; the ventilation volume is 1-4 vvm; the tank pressure is 0.02-0.06 MPa;

and step three, taking the fermentation filtrate, concentrating, standing for 12 hours, adding ethanol solution with the volume fraction of 85% which is 1.2 times of the fermentation filtrate under the stirring state, standing for complete precipitation, taking the supernatant, and performing the steps of sterilization, ultrafiltration membrane filtration, freeze drying and porphyrization to obtain the Xylaria nigripes fermentation product extract.

The fermentation substrate comprises the following components: 10 percent of lactobacillus/soybean compound explant fermentation product, 1.5 percent of glucose, 0.6 percent of magnesium sulfate, 0.4 percent of citric acid and the balance of deionized water.

Example 4 preparation of extract of Xylaria nigripes fermentation product

step two, transferring the culture solution of the Xylaria nigripes seeds in the step one to fermentation containing a fermentation substrate according to 12 v/v%, culturing for 8 days under the conditions that the pH value is 5.3-5.6 and the culture temperature is 25 ℃, and stopping fermentation after the fermentation substrate is filled with hyphae; the stirring speed is 100-200 rpm; the ventilation volume is 1-4 vvm; the tank pressure is 0.02-0.06 MPa;

and step three, taking the fermentation filtrate, concentrating, standing for 12 hours, adding an ethanol solution with the volume fraction of 90% which is 2 times that of the fermentation filtrate under the stirring state, standing for complete precipitation, taking the supernatant, and performing the steps of sterilization, ultrafiltration membrane filtration, freeze drying and grinding to obtain the Xylaria nigripes fermentation product extract.

The fermentation substrate comprises the following components: 8 percent of lactobacillus/soybean compound explant fermentation product, 1.0 percent of glucose, 0.5 percent of magnesium sulfate, 0.4 percent of citric acid and the balance of deionized water.

Example 5 preparation of extract of Xylaria nigripes fermentation product

step two, transferring the culture solution of the Xylaria nigripes seeds in the step one to fermentation containing a fermentation substrate according to 15 v/v%, culturing for 6 days under the conditions that the pH value is 5.3-5.6 and the culture temperature is 25 ℃, and stopping fermentation after the fermentation substrate is filled with hyphae; the stirring speed is 100-200 rpm; the ventilation volume is 1-4 vvm; the tank pressure is 0.02-0.06 MPa;

and step three, taking the fermentation filtrate, concentrating, standing for 12 hours, adding ethanol solution with the volume fraction of 92% which is 1.5 times of the fermentation filtrate under the stirring state, standing for complete precipitation, taking the supernatant, and performing the steps of sterilization, ultrafiltration membrane filtration, freeze drying and porphyrization to obtain the Xylaria nigripes fermentation product extract.

The fermentation substrate comprises the following components: 12 percent of lactobacillus/soybean compound explant fermentation product, 2 percent of glucose, 0.8 percent of magnesium sulfate, 0.5 percent of citric acid and the balance of deionized water.

Examples 6 to 8 eye repair essence and the amount thereof (% by mass)

Note: wherein the Xylaria nigripes fermentation product extracts prepared in examples 6 to 8 correspond to the Xylaria nigripes fermentation product extracts prepared in examples 3 to 5, respectively.

The preparation method comprises the following steps:

putting the phase A into a water phase pot, stirring uniformly, heating and stirring to 80 ℃, and preserving heat for 10 min; putting the phase B into an oil phase pot, heating to 80 ℃, and uniformly stirring; filtering the materials in the water phase pot, pumping into an emulsifying pot, filtering the materials in the oil phase pot, slowly pumping into the emulsifying pot, stirring, and homogenizing for 5 min; cooling to 40 deg.C, adding phase C, stirring, inspecting, discharging, and packaging.

Comparative example 1

The difference from example 6 is that the fermentation substrate used in the first step in the preparation of the extract of Xylaria nigripes fermentation product consists of: 10% by weight of lactobacillus/soy extract fermentation filtrate, 1.5% by weight of glucose, 0.6% by weight of magnesium sulfate, 0.4% by weight of citric acid and the balance deionized water.

Comparative example 2

The difference from example 6 is that the fermentation substrate used in the first step in the preparation of the extract of Xylaria nigripes fermentation product consists of: 1.5% by weight of glucose, 0.6% by weight of magnesium sulfate, 0.4% by weight of citric acid and the balance deionized water.

Comparative example 3

The difference from the example 6 is that the Xylaria nigripes extract prepared in the example 1 in the Chinese patent CN105411951B is used for replacing the Xylaria nigripes fermentation product extract to prepare the eye essence.

Test one, skin irritation test

Referring to the reaction in the multiple skin irritation test method in the cosmetic hygiene Specification (2007 edition), the eye repair essence of examples 6 to 8 was subjected to multiple skin irritation tests, before the tests, back hairs on both sides of the spine of a test animal were cut off, the hair removal range was 2cm × 2cm, the application area was 1.5 × 1.5cm, 2ml of a test sample was applied to one side, a control was made on one side, the test sample was applied once a day, the test sample was continuously applied for 14d, hair was cut before each application from the 2d, the residual test sample was removed with warm water, and the results were observed after 1 h. The test results were scored according to the scoring criteria in the cosmetic hygiene code (2007 edition), the average score per animal per day was calculated, and the skin irritation intensity was judged according to table 1 below, and the test results are shown in table 1 below.

TABLE 1 stimulation intensity rating

Integral mean value	Strength of
		0～＜0.5	Has no irritation
0.5～＜2.0	Light irritation
		2.0～＜6.0	Middle irritation
6.0～8.0	Strong irritation

TABLE 2 results of multiple skin irritation tests

Sample (I)	Mean of daily score for each animal	Intensity of stimulus
			Example 6	0.28	Has no irritation
Example 7	0.22	Has no irritation
			Example 8	0.35	Has no irritation

Test II, removal test of pigmented black eye

1.1 subject inclusion and exclusion criteria: 70 patients with pigmented black eyes were selected, the average age was 33 years, and the selection criteria were: self-evaluation of dark circles; secondly, the eye drops have obvious pigmentary black eye circles evaluated by clinicians, including simple pigmentary black eye circles and mixed black eye circles mainly based on the pigmentary property, and the VISIA facial imaging instrument is adopted to detect the visible obvious pigmentation of the upper eyelid and the lower eyelid. Exclusion criteria: women in pregnancy and lactation; ② people allergic to the components of the product; ③ patients with infection or inflammation around the eyes; fourthly, the scar is obvious around the eyes; fifthly, the patients who use externally applied medicines or similar products in 2 weeks; sixthly, the eye receives any cosmetic treatment in the last half year.

1.2 test methods: the 70 patients were randomly divided into 7 groups, namely, examples 6 to 8 and comparative examples 1 to 3, and the subjects except the blank control group were externally applied with the product 1 time in the morning and evening for 56 days, skin melanin content MI values before use, and on the 28 th and 56 th days of use of the subjects were tested by a German Mexameter MX-18 erythema/melanin index tester, the measurement was repeated 3 times for each measurement, and the average value was averaged to perform statistical difference analysis, and the test results are shown in Table 3.

TABLE 3 skin melanin MI values

Sample (I)	Before use	Day 28	Day 56
				Blank group	263.54±36.44	265.36±30.57	258.16±29.45
Example 6	255.80±21.69	232.18±25.65^*	206.33±19.26^**
				Example 7	261.44±25.07	240.20±23.52^*	218.58±23.82^**
Example 8	252.83±19.31	231.77±28.73^*	205.15±17.51^**
				Comparative example 1	248.72±28.58	235.64±15.39	221.56±20.09^*
Comparative example 2	250.39±26.43	241.42±16.27	229.42±15.68^*
				Comparative example 3	258.95±26.32	238.35±23.94^*	220.25±18.66^*

Note: compared with the traditional Chinese medicine preparation before use,^*P＜0.05；^**p is less than 0.01, and the larger the MI value of the skin melanin content is, the higher the skin melanin content is.

As can be seen from the above table, after 28 days using the eye repair essence of examples 6 to 8 and comparative example 3 of the present invention, the melanin content in the skin of the eyes significantly changed from that before use, and after 56 days using the eye repair essence, the melanin content in the skin of the eyes significantly changed from that before use, indicating that the eye repair essence has an excellent improving effect on a pigmented black eye.

Comparative example 1 the eye essence obtained by using the filtrate of the lactobacillus/soybean extract fermentation product instead of the fermentation product of the lactobacillus/soybean composite explant of the invention has no obvious change in the content of melanin in the eyes before use on the 28 th day and has obvious change after the 56 th day; comparative example 2 functions similarly to comparative example 1; comparative example 3 the Xylaria nigripes extract prepared in example 1 of Chinese patent CN105411951B is used as an effective component to prepare the eye essence, and the eye essence has a good removing effect on pigment black eyes.

Experiment three, vascular black eye removal experiment

1.1 subject inclusion and exclusion criteria: 60 patients with pigmented black eyes were selected, the average age was 32 years, and the selection criteria were: self-evaluation of the red-eye health-care product has dark eye circles, and pink red (oxyhemoglobin) or bluish purple (hemoglobin) appears under sunlight; and secondly, the blood vessel black eye is obvious through evaluation of a clinician, and observation under a wood lamp and comparison under a fluorescent lamp have no obvious change. Exclusion criteria: women in pregnancy and lactation; ② people allergic to the components of the product; ③ patients with infection or inflammation around the eyes; fourthly, the scar is obvious around the eyes; fifthly, the patients who use externally applied medicines or similar products in 2 weeks; sixthly, the eye receives any cosmetic treatment in the last half year.

1.2 test methods: the 60 patients were randomly divided into 6 groups, examples 6 to 8 and comparative examples 1 to 3, and the subjects were externally applied 1 time per day in the morning and evening for 56 days, and skin Erythema Indexes (EI) before and at 56 days were measured using a German Mexameter MX-18 erythema/melanin index tester, and the measurement was repeated 3 times for each measurement, averaged, and subjected to statistical difference analysis, and the results are shown in Table 4.

TABLE 4 skin erythema index test results

Sample (I)	Before use	Day 56
			Example 6	326.21±56.04	268.62±34.28^*
Example 7	308.52±41.26	254.85±39.55^*
			Example 8	289.45±36.11	231.06±41.46^*
Comparative example 1	321.61±48.30	295.93±35.28
			Comparative example 2	298.72±43.24	276.68±40.27
Comparative example 3	314.46±50.56	289.78±38.39

Note: compared with the traditional Chinese medicine preparation before use,^*P＜0.05；^**p is less than 0.01, and the larger the skin EI value is, the higher the skin heme content is.

As can be seen from the above table, after 56 days using the eye repair essence of examples 6 to 8 of the present invention, the value of erythema index on the skin of the eyes was significantly reduced compared to that before the use, which proves that it can be used for improving vascular black eye, while the erythema index of the remaining groups was not significantly lowered compared to that before the use.

Fourth test, moisture retention test

Selecting 60 subjects, wherein the age range is 25-40 years, the average age is 35 years, randomly dividing the subjects into 6 groups, externally applying the eye repair essence products of the invention in the examples 6-8 and the comparative examples 1-3 to each group of subjects, measuring the eye skin moisture content of the subjects after use for 30min, 60min, 2h and 4h by using a CorneometerCM 825 skin moisture content tester, and calculating the skin moisture content increase rate according to the following formula, wherein the test results are shown in the following table 5.

In the formula, MMV₀MMV for pre-use skin; MMV_tIs the skin MMV t period after use.

TABLE 5 moisture content growth rate of eye skin

From the above table, the Xylaria nigripes fermentation product extract obtained by using the lactobacillus/soybean compound explant fermentation product of the invention as the main fermentation substrate for Xylaria nigripes liquid fermentation has more excellent moisturizing effect, the skin water content shows a gradually increasing trend, the peak value is reached 4h after the use, and the effects of instant moisturizing and long-acting moisturizing are achieved.

The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims

1. The eye repair essence is characterized by comprising the following components in percentage by mass: 1-4% of humectant, 0.1-0.2% of thickener, 1-6% of emollient oil, 0.5-2% of emulsifier, 1-12% of Xylaria nigripes fermentation product extract, 0.1-0.2% of preservative, 0.01-0.03% of chelating agent, 0.01-0.02% of essence and the balance of deionized water.

2. The eye repair essence according to claim 1, wherein the Xylaria nigripes fermentation product extract is prepared by taking 1-12% by weight of a lactobacillus/soybean composite explant fermentation product, 0.1-2% by weight of glucose, 0.5-1% by weight of magnesium sulfate, 0.1-0.5% by weight of citric acid and the balance of deionized water as a fermentation substrate, and inoculating 10-15% of Xylaria nigripes for fermentation.

3. The eye repair serum of claim 2 wherein the fermentation substrate consists of: 5-12% of lactobacillus/soybean composite explant fermentation product, 1-2% of glucose, 0.5-0.8% of magnesium sulfate, 0.2-0.5% of citric acid and the balance of deionized water.

4. The eye repair serum of claim 2 or 3 wherein the fermentation process parameters are: the pH value is 5.3-5.6; the culture temperature is 22-25 ℃; the culture time is 6-8 days; the stirring speed is 100-200 rpm; the ventilation volume is 1-4 vvm; the pot pressure is 0.02-0.06 MPa.

5. The eye-repairing essence according to claim 4, further comprising the step of extracting the fermentation filtrate with a solvent, filtering, and freeze-drying to obtain an extract of a fermentation product of Xylaria nigripes.

6. The eye repair serum of claim 5 wherein the solvent is one or both of water and an organic solvent.

7. The eye repair essence according to claim 2 or 3, wherein the lactobacillus/soybean complex explant fermentation product is prepared by the method comprising:

8. The eye repair essence according to claim 7, wherein the preparation of the soybean complex explant comprises the steps of: cleaning and sterilizing soybean seeds, culturing and accelerating germination in a germination culture medium by illumination, and removing seed coats from seed eyes after accelerating germination to obtain the soybean compound explant.

9. A method for preparing the eye-repairing essence according to any one of claims 1 to 8, comprising the steps of: