CN111919107B - 用于定量成像的强度稳定的系统和方法 - Google Patents

用于定量成像的强度稳定的系统和方法 Download PDF

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Publication number
CN111919107B
CN111919107B CN201980009899.4A CN201980009899A CN111919107B CN 111919107 B CN111919107 B CN 111919107B CN 201980009899 A CN201980009899 A CN 201980009899A CN 111919107 B CN111919107 B CN 111919107B
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light
sample
camera
microscopic samples
samples
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Chinese (zh)
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CN111919107A (zh
Inventor
安德里亚斯·格夫罗雷尔
伯恩哈德·申瓦尔德
法兰克·凯德尔
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Molecular Devices Austria GmbH
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Molecular Devices Austria GmbH
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates

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  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
  • Optical Measuring Cells (AREA)
CN201980009899.4A 2018-01-26 2019-01-23 用于定量成像的强度稳定的系统和方法 Active CN111919107B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862622311P 2018-01-26 2018-01-26
US62/622,311 2018-01-26
PCT/IB2019/050578 WO2019145880A1 (en) 2018-01-26 2019-01-23 System and method for intensity stabilization for quantitative imaging

Publications (2)

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CN111919107A CN111919107A (zh) 2020-11-10
CN111919107B true CN111919107B (zh) 2024-05-14

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CN201980009899.4A Active CN111919107B (zh) 2018-01-26 2019-01-23 用于定量成像的强度稳定的系统和方法

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US (1) US11442018B2 (enExample)
EP (1) EP3743712B8 (enExample)
JP (2) JP2021511515A (enExample)
CN (1) CN111919107B (enExample)
WO (1) WO2019145880A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116067934B (zh) * 2023-03-28 2023-07-18 赛默飞世尔（上海）仪器有限公司 用于信号采集的方法和设备
CN116500010B (zh) * 2023-06-25 2024-01-26 之江实验室 荧光显微成像系统及其方法、荧光显微检测装置

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7175500A (en) * 1997-06-09 2001-03-08 Caliper Technologies Corporation Apparatus and methods for correcting for variable velocity in microfluidic systems
CN101793829A (zh) * 2010-02-04 2010-08-04 深圳大学 一种荧光显微成像方法及系统
CN105579828A (zh) * 2013-05-28 2016-05-11 克莫麦特公司 图像式细胞仪

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001001112A1 (en) * 1999-06-26 2001-01-04 Packard Instrument Company, Inc. Microplate reader
US6974938B1 (en) * 2000-03-08 2005-12-13 Tibotec Bvba Microscope having a stable autofocusing apparatus
US20080277595A1 (en) * 2007-05-10 2008-11-13 Pacific Biosciences Of California, Inc. Highly multiplexed confocal detection systems and methods of using same
JP5259207B2 (ja) * 2008-02-05 2013-08-07 オリンパス株式会社 細胞画像解析装置及びその方法並びにそのソフトウェア
JP5365407B2 (ja) * 2009-08-17 2013-12-11 ソニー株式会社 画像取得装置及び画像取得方法
US8466436B2 (en) * 2011-01-14 2013-06-18 Lumencor, Inc. System and method for metered dosage illumination in a bioanalysis or other system
DE102011087196A1 (de) * 2011-11-28 2013-05-29 Leica Microsystems Cms Gmbh Mikroskopbeleuchtungssystem und -verfahren

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7175500A (en) * 1997-06-09 2001-03-08 Caliper Technologies Corporation Apparatus and methods for correcting for variable velocity in microfluidic systems
CN101793829A (zh) * 2010-02-04 2010-08-04 深圳大学 一种荧光显微成像方法及系统
CN105579828A (zh) * 2013-05-28 2016-05-11 克莫麦特公司 图像式细胞仪

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Publication number Publication date
WO2019145880A1 (en) 2019-08-01
JP2023133540A (ja) 2023-09-22
JP2021511515A (ja) 2021-05-06
EP3743712B8 (en) 2023-07-19
US11442018B2 (en) 2022-09-13
EP3743712A1 (en) 2020-12-02
EP3743712B1 (en) 2023-06-07
CN111919107A (zh) 2020-11-10
US20200348235A1 (en) 2020-11-05

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