CN111892455A - Carbon-based bio-enzyme fertilizer for resisting continuous cropping obstacle of flue-cured tobacco - Google Patents
Carbon-based bio-enzyme fertilizer for resisting continuous cropping obstacle of flue-cured tobacco Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The invention belongs to the field of agricultural planting, and particularly relates to a carbon-based biological enzyme fertilizer for resisting continuous cropping obstacles of flue-cured tobaccos, which comprises an organic fertilizer, biochar and ferment, wherein the organic fertilizer is prepared from cow dung, oil cake, fungus bags, straws, phosphorus elements and beneficial bacteria. The carbon-based bio-enzyme fertilizer applied to the acid red soil and used for resisting the continuous cropping obstacle of the flue-cured tobacco can provide medium and trace elements required by the growth of the tobacco, adsorb toxic and harmful substances in the soil, inhibit the activity of harmful floras in the soil, increase the number of beneficial floras, improve the soil fertility, improve the physicochemical property of the soil, loosen and fertilize the soil, and further improve the disease resistance and the yield quality of the tobacco.
Description
Technical Field
The invention belongs to the field of agricultural planting, and particularly relates to a carbon-based bio-enzyme fertilizer for resisting continuous cropping obstacles of flue-cured tobacco.
Background
The special fertilizer for crops is a fertilizer which is specially used for the crops in the region by scientifically proportioning the nutrient elements such as nitrogen, phosphorus, potassium, medium and trace elements according to the soil condition in the region and the fertilizer requirement characteristics of the crops. The special fertilizer for crops is developed and produced according to the growth and development rules of crops and the absorption amount of different elements, various special fertilizer compound fertilizers for crops are developed according to local habit fertilization levels, cultivation systems and natural climate conditions by analyzing the characteristics of soil nutrient content and according to fertilizer requirement rules of different crops, and various beneficial trace elements and irritant substances capable of promoting the growth of crops are added into the fertilizer for different crops and different soils, and meanwhile, a technology matched service technology is implemented, so that the special fertilizer for crops is gradually one of the methods for solving the problems of low fertilizer utilization rate, crop yield and quality reduction and the like.
Tobacco is one of the important economic crops in China, and the planting area and the total yield of the tobacco are the first in the world. At present, the tobacco crop planting in China mainly depends on the application of a large amount of chemical fertilizers and pesticides to keep the high yield of tobacco leaves, so that the soil environment is seriously deteriorated, and the continuous cropping obstacle becomes one of the key factors restricting the tobacco planting industry in Yunnan and even China. Continuous cropping of flue-cured tobacco can cause increase of soil nutrient residue, enrichment of salt, reduction of activity of soil carbon nitrogen metabolism related enzyme, enrichment of a few fungus groups due to accumulation of phenol acidification susceptible substances at the root of planted tobacco, reduction of the number of most fungus, bacteria and actinomycete groups, obvious simplification trend of population change, unbalance of soil micro-ecological structure, outbreak of continuous cropping diseases, withering and death of flue-cured tobacco and vicious circle. Therefore, a method capable of realizing continuous cropping of flue-cured tobacco is urgently needed.
Disclosure of Invention
In order to solve the technology, the invention can solve the problems of disease outbreak and withering and death of the flue-cured tobacco in continuous cropping by using the carbon-based bio-enzyme fertilizer for resisting the continuous cropping obstacle of the flue-cured tobacco.
The specific technical scheme is as follows: a charcoal-based bio-enzyme fertilizer for resisting continuous cropping obstacles of flue-cured tobaccos comprises an organic fertilizer, biochar and enzymes, wherein the organic fertilizer is prepared from cow dung, oil cakes, fungus bags, straws, phosphorus elements and beneficial bacteria.
Further, the beneficial bacteria comprise bacillus subtilis, trichoderma and actinomycetes. The ratio of the bacillus subtilis to the trichoderma to the actinomycetes is 1:1:1 by weight.
Further, cow dung, oil cake, fungus bags, straws, phosphorus elements and beneficial bacteria in the organic fertilizer are mixed according to the weight ratio of 3: 2: 2: 2: 1: 0.01.
further, the preparation method comprises the following steps:
(1) preparing an organic fertilizer:
the organic fertilizer is prepared by mixing 30% of cow dung, 20% of oil cake, 20% of fungus bag, 20% of straw and 10% of phosphorus element, adding a proper amount of bacillus subtilis, trichoderma and actinomycetes, and fermenting for 15-25 days under the conditions that the temperature is 65-70 ℃ and the humidity is 60-65%, so as to obtain the organic fertilizer special for tobacco;
(2) preparing a carbon-based biological enzyme fertilizer:
45 percent of the prepared organic fertilizer is mixed with 45 percent of biochar and 10 percent of ferment with the same quantity; uniformly mixing the biochar and the organic fertilizer, spraying the ferment in a spraying mode while stirring until the enzymes are uniformly mixed, and fermenting for about 20-30 days under the conditions that the temperature is kept at 65-70 ℃ and the humidity is 60-65% to obtain the carbon-based bio-enzyme fertilizer.
Has the advantages that:
biochar is a charcoal used as a soil conditioner, can help plant growth, can be applied to agricultural use and carbon collection and storage, and is different from the traditional charcoal generally used as fuel. The biochar can improve the organic carbon content level of soil; the volume weight of the clay soil can be reduced, and the soil texture and the farming performance are improved; the biochar can obviously improve the pH value of the soil; because the biochar is rich in aromatic ring structures, hydroxycarboxylates and other groups, ion exchange sites are obviously increased, and the surface exchange activity of the biochar is higher, the CEC level of the soil is obviously improved after the biochar is applied, and the CEC improvement effect on low CEC and acid soil is very obvious; after the biochar is applied, the water holding capacity and the water supply capacity of the soil are improved; the pores and the surface of the biochar can provide a microenvironment for microorganisms to inhabit, so that the number and the activity of certain microorganisms are increased; the unique surface characteristics of the biochar enable the biochar to have a strong adsorption effect on soil nutrients, reduce nutrient leaching and fixing loss, promote nutrient absorption of plants, improve the utilization rate of the fertilizer and have a yield increasing effect.
The environment-friendly ferment is one kind of ferment, is brown liquid produced by anaerobic fermentation of kitchen wastes (fresh garbage) mixed with sugar and water, and has good environment-friendly effects of purifying a sewer, purifying air and the like. The ferment is a high molecular substance with a biological catalysis function, and can promote metabolism, improve immunity and generate energy. The ferment in the invention is a microbial preparation which is prepared by fermenting plants such as fresh vegetables and fruits and is rich in enzyme, vitamins, minerals and secondary metabolites. The fermentation of the environment-friendly ferment can decompose residual hormones, antibiotics and other medicines of animal manure, kill various germs and parasitic ova, increase soil organic matters, promote the growth of soil microorganisms, enhance the activity of soil and solve the problem of poor soil caused by long-term use of chemical fertilizers.
The oil cake is residue obtained by squeezing various seeds containing more oil components and removing oil, contains high nitrogen and phosphorus nutrients, 4-6 percent of nitrogen and about 2 percent of phosphorus, is stacked and fermented with pig manure, has lasting fertility and good effect, and can improve the fertility of soil and prevent soil hardening.
Active substances such as subtilin, polymyxin, nystatin, gramicidin and the like generated in the growth process of bacillus subtilis have obvious inhibiting effect on pathogenic bacteria or conditional pathogenic bacteria of endogenous infection; trichoderma can produce various bioactive substances with antagonistic action on plant pathogenic fungi, bacteria and insects, such as cell wall degrading enzymes and secondary metabolites, can improve the stress resistance of crops, promote plant growth and improve the yield of agricultural products, and is widely used for biological control, biological fertilizer and soil conditioner; the actinomycetes can decompose a plurality of organic fertilizers, including complex compounds such as aromatic compounds, paraffin, rubber, cellulose, wood and the like and some compounds with strong toxicity such as cyanogen and the like. Therefore, the actinomycetes not only have positive effects in natural substance circulation, but also in biological treatment of sewage and organic solid waste, and can promote soil to form a granular structure so as to improve soil.
The organic fertilizer is prepared from cow dung serving as a raw material, the cost is low, the quality is stable, and the cow dung is rich in various nutrients: 1) promoting the yield increase of crops: the organic fertilizer prepared from the cow dung contains rich organic matters, can comprehensively provide crop nitrogen, phosphorus and potassium and various medium and trace elements, greatly improves the quality of agricultural products, is sweet in melon, vegetable and rice after being applied, can effectively improve the physical and chemical properties of soil, loosens the soil, enhances the air permeability of the soil and greatly improves the soil fertility.
2) The components of the biological organic fertilizer made of cow dung comprise azotobacter, phosphate solubilizing bacteria, potassium solubilizing bacteria and the like, and the biological organic fertilizer can transform nutrient substances in soil to be used by crops after being applied, so that the nutrient substances are continuously supplied to the crops. 3) Applying the fertilizer effectively promotes the growth of crops: the application of the organic fertilizer prepared from cow dung can lead a large amount of beneficial active bacteria to be rapidly propagated, effectively inhibit the growth of harmful microorganisms, simultaneously generate a plurality of enzymes, promote the robust growth of crops and enhance the stress resistance and the disease and insect resistance of the crops. 4) The land capability is effectively improved: a large amount of beneficial active bacteria in the organic fertilizer directly participate in the processes of soil substance and energy conversion, humus formation and decomposition and the like, play a role in gluing a soil granular structure, effectively improve the physical properties of soil, enhance the air permeability, water retention and fertilizer retention capacity of the soil, prevent the soil from hardening and acidifying, fertilize the soil, improve the fertilizer utilization rate and reduce the fertilizer application cost, thereby achieving the aim of virtuous circle of the soil.
According to the invention, the organic fertilizer is mixed and fermented with the biochar and the ferment after being mixed and fermented with the cow dung, the oil cake, the fungus bag, the straw, the phosphorus element and the beneficial bacteria, so that the obtained carbon-based bio-enzyme fertilizer can overcome the continuous cropping obstacle of the acid red soil to resist the flue-cured tobacco, can provide medium and trace elements required by the growth of the tobacco, can adsorb toxic and harmful substances in the soil, inhibit the activity of harmful flora in the soil, increase the number of the beneficial flora, improve the soil fertility, improve the physical and chemical properties of the soil, and enable the soil to become loose and fertile, thereby improving the disease resistance and the yield quality of the tobacco.
Detailed Description
Example 1
(1) Preparing an organic fertilizer:
the organic fertilizer is prepared by mixing 30% of cow dung, 20% of oil cake, 20% of fungus bag, 20% of straw and 10% of phosphorus element, adding a proper amount of bacillus subtilis, trichoderma and actinomycetes, and fermenting for 20 days under the conditions that the temperature is 65-70 ℃ and the humidity is 60-65%, so as to obtain the organic fertilizer special for tobacco;
(2) preparing a carbon-based biological enzyme fertilizer:
45 percent of the prepared organic fertilizer is mixed with 45 percent of biochar and 10 percent of ferment with the same quantity; uniformly mixing the biochar and the organic fertilizer, spraying the ferment in a spraying mode while stirring until the biochar and the organic fertilizer are uniformly mixed, and fermenting for 25 days under the conditions that the temperature is kept at 65-70 ℃ and the humidity is 60-65% to obtain the carbon-based bio-enzyme fertilizer.
Example 2
A control group 1, in which a common organic fertilizer is used as a fertilizer, continuous cropping flue-cured tobacco is carried out on a flue-cured tobacco planting land, corresponding data monitoring is carried out, and an average value is obtained;
control group 2: taking a common organic fertilizer as a fertilizer, spraying 10% of environment-friendly enzyme, fermenting for 25 days under the conditions of keeping the temperature at 65-70 ℃ and the humidity at 60-65%, continuously cropping flue-cured tobacco on a flue-cured tobacco planting land, applying the fertilizer, monitoring corresponding data, and taking an average value;
control group 3: taking a common organic fertilizer as a fertilizer, applying 0.1% of bacillus subtilis, trichoderma and actinomycetes in a matching manner, carrying out continuous cropping flue-cured tobacco on a flue-cured tobacco planting land, applying the fertilizer, carrying out corresponding data monitoring, and taking an average value;
control group 4: taking a common organic fertilizer as a fertilizer, applying 45% of charcoal in a matching manner, fermenting for 25 days under the conditions of keeping the temperature at 65-70 ℃ and the humidity at 60-65%, continuously cropping flue-cured tobacco on the ground where the flue-cured tobacco is planted, applying the fertilizer, monitoring corresponding data, and taking an average value;
control group 5: using a common organic fertilizer as a fertilizer, spraying plant vigor elements after field planting, fermenting for 25 days under the conditions that the temperature is kept at 65-70 ℃ and the humidity is 60-65%, continuously cropping flue-cured tobacco on the ground where the flue-cured tobacco is planted, applying the fertilizer, carrying out corresponding data monitoring, and taking an average value;
experimental group 1: the organic fertilizer is prepared by mixing 30% of cow dung, 20% of oil cake, 20% of fungus bag, 20% of straw and 10% of phosphorus element, adding a proper amount of bacillus subtilis, trichoderma and actinomycetes, and fermenting for 20 days at the temperature of 65-70 ℃ and the humidity of 60-65%, so as to obtain the special organic fertilizer for tobacco as the fertilizer. And (4) carrying out continuous cropping flue-cured tobacco on the flue-cured tobacco planted land, and carrying out corresponding data monitoring.
Experimental group 2: mixing an organic fertilizer with 30% of cow dung, 20% of oil cake, 20% of fungus bag, 20% of straw and 10% of phosphorus element, adding a proper amount of bacillus subtilis, trichoderma and actinomycetes, and fermenting for 20 days under the conditions that the temperature is 65-70 ℃ and the humidity is 60-65% for [1] to obtain the organic fertilizer special for tobacco; 45 percent of the prepared organic fertilizer is mixed with 45 percent of biochar and 10 percent of ferment with the same quantity; after the biochar and the organic fertilizer are uniformly mixed, spraying the ferment in a spraying mode while stirring until the enzymes are uniformly mixed, and fermenting for 25 days under the conditions that the temperature is kept at 65-70 ℃ and the humidity is 60-65% to obtain the carbon-based bio-enzyme fertilizer as the fertilizer. And (4) carrying out continuous cropping flue-cured tobacco on the flue-cured tobacco planted land, and carrying out corresponding data monitoring.
Experimental requirements
The test is a field test, and the test site is a Jingjing Xuanwei drowning town with 1 mu in total, namely 667m2Is divided into 7And (3) carrying out test planting on the blocks, respectively applying fertilizers of the control groups 1, 2, 3, 4 and 5 and the experimental groups 1 and 2, and applying the organic fertilizer for 0.25 ton per mu and 500 plants per mu, namely applying the fertilizer for 500g per mu. [2]
Control group 2: applying general organic fertilizer, using the same amount as the control group 1, and spraying environment-friendly enzyme after field planting.
③ control group 3: applying common organic fertilizer with the same amount as the control group 1, and applying bacillus subtilis, trichoderma and actinomycetes in a matching way.
Comparison group 4: applying general organic fertilizer with the same dosage as the control group 1, and applying 300 g of biochar to each plant.
Control group 5: applying common organic fertilizer with the same amount as the control group 1, and spraying plant vigor elements after planting.
Experimental group 1: the organic fertilizer special for tobacco prepared by the self is applied, wherein the organic fertilizer is applied by 0.25 ton per mu and 500 plants per mu, namely the using amount of each flue-cured tobacco is 500 g.
Experiment set 2: the carbon-based bio-enzyme fertilizer special for tobacco, which is prepared by us, is applied, wherein the organic fertilizer is applied by 0.25 ton per mu, and the amount of the organic fertilizer is 500 plants per mu, namely the amount of the flue-cured tobacco is 500g per mu. [3]
The technical points are as follows:
1. the requirements of soil preparation, shed building and planting specification
(1) Soil: the soil layer is deep and loose, and sandy loam with high organic matter content is suitable.
(2) And (3) ploughing requirement: deep ploughing, breaking soil and drying in the sun.
(3) And (5) making beds. The plot (button) is taken as a unit to make the plots along the slope direction, the plots are required to be leveled and fine, and the height of the plots is more than 20 cm.
(4) Planting specification: the plant spacing is 45cm, and the row spacing is 45 cm.
And fertilization requirements are as follows:
and (3) digging a large pond, uniformly mixing the uniformly dispersed topsoil fertilizer into soil by using a hoe, and uniformly mixing the layer with the fertilizer and the soil to a depth of 15-20 cm.
And field management
After planting, 300 times of Qianzhou phytochemicals are sprayed 1 time per month.
Investigating and sampling soil samples in each stage of tobacco, wherein the sampling method is a five-point sampling method, konjak rhizosphere soil is sampled, one mixed sample is sampled in each treatment, and each sample is repeated for 3 times in later-stage soil analysis.
Measurement index
1. The incidence rate is counted by visual observation, and the number of plants with the incidence (such as root black rot, bacterial wilt, black shank and the like) is recorded according to field investigation.
2. The pH value of the soil can be accurately known by using a pH meter for measuring;
3. the total amount of soil microorganisms can be known through the traditional flat culture method for culturing and counting, the soil microorganisms can promote the decomposition of organic matters and the conversion of nutrients in soil, and the richer the microorganisms are, the stronger the self-regulation capability of the soil is.
4. The soil phenolic acid substance is obtained by measuring the content of the phenolic acid substance in soil by using a WATERS ultra-high performance liquid phase, wherein cinnamic acid, 4-coumaric acid, p-hydroxybenzoic acid, vanillic acid, ferulic acid and cinnamic acid are mainly measured. Phenolic acid substances are important factors causing continuous cropping obstacles, and the continuous cropping obstacles are more serious when the content is higher.
Experimental data and analysis
As can be seen from the following data, the incidence, pH, total microbial content and phenolic acid substances of flue-cured tobacco are greatly different when the carbon-based bio-enzyme fertilizer and the common organic fertilizer are applied. The incidence of disease can be seen from the data: applying a general organic fertilizer (a control group 1) > applying a general organic fertilizer + an environment-friendly enzyme (a control group 2) > applying a general organic fertilizer + charcoal (a control group 4) > applying a general organic fertilizer + a phyto-activin (a control group 5) > applying a general organic fertilizer + bacillus subtilis, trichoderma, actinomycetes (a control group 2) > applying a tobacco-dedicated organic fertilizer (an experimental group 1) > applying a tobacco-carbon-based bio-enzyme fertilizer (an experimental group 2), which shows that the environment-friendly enzyme, the biochar, the bacillus subtilis, the trichoderma, the actinomycetes and the phyto-activin can obviously reduce the morbidity of flue-cured tobacco, the phyto-activin and the bacillus subtilis, the trichoderma and the actinomycetes are better, the bio-enzyme fertilizer is added with the bacillus subtilis, the trichoderma and the actinomycetes, has obvious inhibiting effect on endogenous pathogenic bacteria or conditional pathogenic bacteria infection, and reduces the number of harmful microorganisms and pests in soil, after the plant vigor element is sprayed, the resistance of the plant can be effectively enhanced, so that the morbidity can be greatly reduced; the charcoal can effectively adjust the pH value of soil and improve the soil environment, and the pH value of the soil applied with the charcoal-based bio-enzyme fertilizer is 0.35 higher than that of the soil applied with a common organic fertilizer; because the microbial bags and the environment-friendly enzymes are added, and various components are matched for use, the soil environment is improved, the total amount of soil microorganisms is obviously improved, and the total amount of the soil microorganisms applying the carbon-based biological enzyme fertilizer is 2.38 times that of the soil microorganisms applying the common organic fertilizer; the phenolic acid substances of the soil applied with the carbon-based bio-enzyme fertilizer are lower than those of the soil applied with the common organic fertilizer, which shows that the continuous cropping obstacle of the soil is obviously reduced. Therefore, the carbon-based bio-enzyme fertilizer is more suitable for the growth of flue-cured tobacco than common organic fertilizers, can promote the growth of soil microorganisms, enriches the total amount of the microorganisms and increases beneficial flora in soil; the morbidity of the plants is greatly reduced; reducing the content of phenolic acid substances in the soil; the pH value of the soil can be improved, and the pH value of the soil can be adjusted; improving soil fertility, enhancing disease resistance of the flue-cured tobacco, keeping soil continuity and providing nutrients required by the growth of the flue-cured tobacco, thereby increasing the yield of the flue-cured tobacco and improving the quality of the flue-cured tobacco.
Claims (4)
1. The carbon-based bio-enzyme fertilizer for resisting continuous cropping obstacles of flue-cured tobaccos is characterized by comprising an organic fertilizer, biochar and enzymes, wherein the organic fertilizer is prepared from cow dung, oil cake, fungus bags, straws, phosphorus elements and beneficial bacteria.
2. The carbon-based bio-enzyme fertilizer for resisting continuous cropping obstacles of flue-cured tobaccos as claimed in claim 1, wherein the beneficial bacteria comprise bacillus subtilis, trichoderma and actinomycetes.
3. The carbon-based bio-enzyme fertilizer for resisting continuous cropping obstacles of flue-cured tobaccos, as claimed in claim 1, wherein cow dung, oil cakes, fungus bags, straws, phosphorus elements and beneficial bacteria are contained in the organic fertilizer in a weight ratio of 3: 2: 2: 2: 1: 0.01.
4. the carbon-based bio-enzyme fertilizer for resisting continuous cropping obstacles of flue-cured tobaccos, as claimed in claim 3, is prepared by the following steps:
(1) preparing an organic fertilizer:
the organic fertilizer is prepared by mixing 30% of cow dung, 20% of oil cake, 20% of fungus bag, 20% of straw and 10% of phosphorus element, adding a proper amount of bacillus subtilis, trichoderma and actinomycetes, and fermenting for 15-25 days under the conditions that the temperature is 65-70 ℃ and the humidity is 60-65%, so as to obtain the organic fertilizer special for tobacco;
(2) preparing a carbon-based biological enzyme fertilizer:
45 percent of the prepared organic fertilizer is mixed with 45 percent of biochar and 10 percent of ferment with the same quantity; uniformly mixing the biochar and the organic fertilizer, spraying the ferment in a spraying mode while stirring until the biochar and the organic fertilizer are uniformly mixed, and fermenting for 20-30 days under the conditions that the temperature is kept at 65-70 ℃ and the humidity is 60-65% to obtain the carbon-based bio-enzyme fertilizer.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115141054A (en) * | 2021-08-10 | 2022-10-04 | 中国热带农业科学院南亚热带作物研究所 | Preparation method and application of double-fermentation type biochar-based organic fertilizer |
| CN115152568A (en) * | 2022-06-14 | 2022-10-11 | 安徽省农业科学院烟草研究所 | Method for repairing continuous cropping obstacles of tobacco field |
| CN115286472A (en) * | 2022-10-08 | 2022-11-04 | 云南硕农农业科技有限公司 | Activating agent for improving self-repairing capability of soil as well as preparation method and application thereof |
| CN115747249A (en) * | 2022-11-28 | 2023-03-07 | 湖南大学 | Application of tobacco NtabCrRLK12 gene in relieving tobacco continuous cropping obstacle |
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| AU2009101249A4 (en) * | 2009-12-04 | 2010-02-25 | O'grady Rural Management Services Pty Ltd | A substantially odourless fertilizer made from organic waste |
| CN102344326A (en) * | 2011-08-15 | 2012-02-08 | 河海大学 | Organic biofertilizer capable of inhibiting continuous cropping obstacle of flue-cured tobaccos |
| CN107353164A (en) * | 2017-09-11 | 2017-11-17 | 四川农业大学 | A kind of fermented type charcoal base biological organic fertilizer and preparation method thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| AU2009101249A4 (en) * | 2009-12-04 | 2010-02-25 | O'grady Rural Management Services Pty Ltd | A substantially odourless fertilizer made from organic waste |
| CN102344326A (en) * | 2011-08-15 | 2012-02-08 | 河海大学 | Organic biofertilizer capable of inhibiting continuous cropping obstacle of flue-cured tobaccos |
| CN107353164A (en) * | 2017-09-11 | 2017-11-17 | 四川农业大学 | A kind of fermented type charcoal base biological organic fertilizer and preparation method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115141054A (en) * | 2021-08-10 | 2022-10-04 | 中国热带农业科学院南亚热带作物研究所 | Preparation method and application of double-fermentation type biochar-based organic fertilizer |
| CN115152568A (en) * | 2022-06-14 | 2022-10-11 | 安徽省农业科学院烟草研究所 | Method for repairing continuous cropping obstacles of tobacco field |
| CN115286472A (en) * | 2022-10-08 | 2022-11-04 | 云南硕农农业科技有限公司 | Activating agent for improving self-repairing capability of soil as well as preparation method and application thereof |
| CN115747249A (en) * | 2022-11-28 | 2023-03-07 | 湖南大学 | Application of tobacco NtabCrRLK12 gene in relieving tobacco continuous cropping obstacle |
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