CN111837917B - Method for soilless culture of gastrodia elata - Google Patents
Method for soilless culture of gastrodia elata Download PDFInfo
- Publication number
- CN111837917B CN111837917B CN202010646850.4A CN202010646850A CN111837917B CN 111837917 B CN111837917 B CN 111837917B CN 202010646850 A CN202010646850 A CN 202010646850A CN 111837917 B CN111837917 B CN 111837917B
- Authority
- CN
- China
- Prior art keywords
- gastrodia elata
- branches
- culture
- material layer
- armillaria mellea
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/58—1,2-Diazines; Hydrogenated 1,2-diazines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Mycology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for soilless gastrodia cultivation, which comprises the following steps: (1) culturing armillaria mellea branches; (2) preparing germination bacteria; (3) culturing the gastrodia elata; (4) and (5) cultivating and managing the gastrodia elata. The method for soilless culture of the gastrodia elata can obviously improve the yield of the gastrodia elata and the content of gastrodin in the gastrodia elata; the culture medium provided by the invention is used for cultivating the Armillaria mellea suitable for the culture medium, artificially providing growth nutrition for the Armillaria mellea, ensuring that the Armillaria mellea produced by the culture medium has short growth period, and the mycelia have very good whiteness, coarseness and uniformity, and can effectively improve the yield of the Gastrodia elata Blume and the content of gastrodin in the Gastrodia elata Blume; by using the plant growth regulator applicable to the invention, the yield of the gastrodia elata and the content of gastrodin in the gastrodia elata are obviously improved.
Description
Technical Field
The invention relates to the technical field of soilless culture of plants, in particular to a method for soilless culture of gastrodia elata.
Background
Rhizoma gastrodiae, also called as rhizoma dioscoreae, is a perennial herb of the genus gastrodiae of the family orchidaceae, is distributed in most areas of China, is a relatively common and valuable Chinese medicinal material, before the 70 th century, commercial rhizoma gastrodiae mainly depends on wild, the market demand of rhizoma gastrodiae is increasing along with the understanding of the nutritional value of the rhizoma gastrodiae by people, and the requirement of people cannot be met by simply depending on wild resources. After the 70 s in the 20 th century, the scientists made their efforts to change the wild gastrodia elata into families, and the families of gastrodia elata became the main commodity source.
The main component with higher content in the gastrodia elata is gastrodin which is also called gastrodin, and the gastrodin has the main function and indications: stop endogenous wind and arrest convulsions. It can be used for treating vertigo, black eye, headache, numbness of limbs, hemiplegia, slurred speech, and infantile convulsion. At present, the yield of the soilless culture method of the gastrodia elata needs to be improved, and the gastrodin content is low.
Disclosure of Invention
The invention provides a method for soilless culture of gastrodia elata, which can effectively improve the yield of the gastrodia elata and the content of gastrodin in the gastrodia elata.
The invention adopts the following technical scheme for solving the technical problems:
a method for soilless culture of gastrodia elata comprises the following steps:
(1) culturing armillaria mellea branches;
(2) preparing germination bacteria;
(3) and (3) culturing the gastrodia elata: preparing an incubator, laying a first culture material layer at the bottom of the incubator, wherein the thickness of the first culture material layer is 4-8 cm, laying a second culture material layer above the first culture material layer, wherein the thickness of the second culture material layer is 2-4 cm, uniformly mixing germination bacteria and gastrodia elata seeds, laying germination bacteria seed mixed layers above the second culture material layer to form the germination bacteria seed mixed layers, laying the germination bacteria seed mixed layers with the thickness of 0.2-0.6 cm, uniformly placing armillaria mellea fungus branches above the germination bacteria seed mixed layers, covering the armillaria mellea fungus branches by using a coarse sand layer for 2-4 cm, uniformly spreading a plant growth regulator above the coarse sand layer, and then covering by using leaves;
(4) and (3) gastrodia elata cultivation management: timely watering the incubator, controlling the water inside the incubator at 55-60% and controlling the temperature inside the incubator at 22-28 ℃.
The invention cultures the gastrodia elata from zero generation seeds of the gastrodia elata, the gastrodia elata is an orchid plant which is highly specialized and has no roots and green leaves, the gastrodia elata cannot independently survive under natural conditions, the nutrition is provided by thalli of armillaria mellea, the germination bacteria can be used for embryo of the gastrodia elata seeds, nutrition is provided for the development and growth of the embryo of the gastrodia elata seeds, and the germination rate of the gastrodia elata seeds is improved.
As a preferable scheme, the culture method of the armillaria mellea branches comprises the following steps:
s1: selecting branches of a fresh oak tree with the diameter of 10-15 cm, cutting the fresh oak tree into small sections of branches with the diameter of 30-50 cm, and cutting two ends of the small sections of branches into inclined planes;
s2: soaking the small sections of branches in a nutrient solution for 1-3 h, and drying until the water content of the small sections of branches is 40-55%;
s3: and (3) putting the small sections of branches into a container, adding a culture solution, inoculating halimasch mother seeds on the small sections of branches, and culturing for 25-35 days at the temperature of 22-28 ℃ under an aseptic condition.
The armillaria mellea mainly lives on trees, the trees are used as nutrition, the mixed bacteria on fresh tree stems are less, the fresh trees are selected to culture the armillaria mellea, two ends of small sections of branches are cut into inclined planes, the armillaria mellea can be immersed, and the nutrient solution can provide nitrogen sources and growth factors for the armillaria mellea; the culture solution can provide a plurality of nutrients and growth factors.
As a preferable scheme, the nutrient solution is 0.6-1% of urea aqueous solution.
As a preferred embodiment, the culture solution comprises: 12-15 g of glucose, 0.5-1 g of monopotassium phosphate, 0.1-0.2 g of magnesium sulfate, 1-3 mg of zinc sulfate, 2-5 mg of manganese sulfate, 1-3 mg of boric acid, 0.08-0.12 mg of sodium molybdate, 0.01-0.15 mg of copper sulfate and 0.01-0.05 mg of glutamic acid, and adding water to 1 liter.
As a most preferred embodiment, the culture solution comprises: 14g of glucose, 0.9g of monopotassium phosphate, 0.15g of magnesium sulfate, 2.8mg of zinc sulfate, 4mg of manganese sulfate, 2.5mg of boric acid, 0.11mg of sodium molybdate, 0.08mg of copper sulfate and 0.04mg of glutamic acid, and water is added to the mixture to reach 1 liter.
The armillaria mellea cultured by the culture solution has short growth period, and the hypha has very good whiteness, coarseness and uniformity, when the gastrodia elata is planted, the spawn running time is short, and the excellent armillaria mellea stick (branch) is an important guarantee for obtaining high-yield and high-quality production effect in the production of the gastrodia elata.
As a preferred scheme, the germination bacteria are Osmunda japonica.
As a preferred scheme, the first culture material is wood chip powder and straw powder according to a weight ratio of 1: 0.6 to 1.
As a preferable scheme, the second culture material layer is prepared from corncob particles and perlite according to a weight ratio of 1: 1-2 mixing.
Corncob particles, perlite, straw powder and sawdust powder can provide nutrient elements such as nitrogen, phosphorus and potassium which are beneficial to growth of armillaria mellea, and can also generate a large amount of organic acid, the organic acid can inhibit growth of mould, more importantly, the organic acid can adjust the pH value of soil, so that the pH value of the soil is reduced, and the growth of gastrodia elata and armillaria mellea is facilitated.
As a preferable scheme, the use amount of the plant growth regulator is 40-60 g/m2。
As a preferable scheme, the plant growth regulator is gibberellin, cynanchum, indolebutyric acid and diethyl aminoethyl hexanoate according to the weight ratio of 1: 0.8-1.2: 0.8-1.2: 0.8 to 1.2.
The inventor of the application finds that the plant growth regulator consisting of gibberellin, cynanchum, indolebutyric acid and diethyl aminoethyl hexanoate can greatly promote germination of the gastrodia elata by adding the plant growth regulator above the coarse sand layer, and can effectively improve the yield of the gastrodia elata and the content of gastrodin.
The invention has the beneficial effects that: (1) the method for cultivating the gastrodia elata without soil can obviously improve the yield of the gastrodia elata and the content of gastrodin in the gastrodia elata; (2) the culture medium provided by the invention is used for cultivating the Armillaria mellea suitable for the culture medium, artificially providing growth nutrition for the Armillaria mellea, ensuring that the Armillaria mellea produced by the culture medium has short growth period, and the mycelia have very good whiteness, coarseness and uniformity, and can effectively improve the yield of the Gastrodia elata Blume and the content of gastrodin in the Gastrodia elata Blume; (3) by using the plant growth regulator applicable to the invention, the yield of the gastrodia elata and the content of gastrodin in the gastrodia elata are obviously improved.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are a part of the embodiments of the present invention, but not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A method for soilless culture of gastrodia elata comprises the following steps:
(1) culturing the armillaria mellea branches;
(2) preparing cultivation of the osmunda japonica;
(3) and (3) culturing the gastrodia elata: preparing an incubator, paving a first culture material layer at the bottom of the incubator, wherein the thickness of the first culture material layer is 5cm, paving a second culture material layer above the first culture material layer, wherein the thickness of the second culture material layer is 3cm, uniformly mixing germination bacteria and gastrodia elata seeds, paving the mixture above the second culture material layer to form a germination bacteria seed mixed layer, paving the thickness of the germination bacteria seed mixed layer to be 0.3cm, uniformly placing armillaria mellea fungus branches above the germination bacteria seed mixed layer, covering the armillaria mellea fungus branches by using a coarse sand layer for 3cm, uniformly scattering a plant growth regulator above the coarse sand layer, and then covering the plant growth regulator with leaves;
(4) and (3) gastrodia elata cultivation management: timely watering the incubator, controlling the water inside the incubator at 55-60% and controlling the temperature inside the incubator at 25 ℃.
The culture method of the Armillaria mellea branches comprises the following steps:
s1: selecting branches of a fresh oak tree with the diameter of 12cm, cutting the fresh oak tree into small sections of branches with the diameter of 38cm, and cutting two ends of the small sections of branches into inclined planes;
s2: soaking the small sections of branches in 0.8% urea water solution for 2h, and drying until the water content of the small sections of branches is 40-55%;
s3: placing the branch into a container, adding culture solution, inoculating Armillaria mellea mother strain on the branch, and culturing at 25 deg.C under aseptic condition for 32 days.
The culture solution comprises: 14g of glucose, 0.9g of monopotassium phosphate, 0.15g of magnesium sulfate, 2.8mg of zinc sulfate, 4mg of manganese sulfate, 2.5mg of boric acid, 0.11mg of sodium molybdate, 0.08mg of copper sulfate and 0.04mg of glutamic acid, and water is added to the mixture to reach 1 liter.
The first culture material is wood chip powder and straw powder according to the weight ratio of 1: 0.8 and mixing.
The second culture material layer is formed by mixing corncob particles and perlite according to a weight ratio of 1: 1.5 mixing.
The using amount of the plant growth regulator is 45g/m2。
The plant growth regulator is prepared from gibberellin, cynarin, indolebutyric acid and diethyl aminoethyl hexanoate according to the weight ratio of 1: 1: 1: 1 and mixing.
Example 2
A method for soilless culture of gastrodia elata comprises the following steps:
(1) culturing the armillaria mellea branches;
(2) preparing cultivation of the osmunda japonica;
(3) and (3) culturing the gastrodia elata: preparing an incubator, laying a first culture material layer at the bottom of the incubator, wherein the thickness of the first culture material layer is 8cm, laying a second culture material layer above the first culture material layer, wherein the thickness of the second culture material layer is 4cm, uniformly mixing germination bacteria and gastrodia elata seeds, and laying the mixture layer above the second culture material layer to form a germination bacteria seed mixture layer, wherein the laying thickness of the germination bacteria seed mixture layer is 0.6cm, uniformly placing armillaria mellea branches above the germination bacteria seed mixture layer, covering the armillaria mellea branches by using a coarse sand layer for 4cm, uniformly spreading a plant growth regulator above the coarse sand layer, and then covering the plant growth regulator with leaves;
(4) and (3) gastrodia elata cultivation management: timely watering the incubator, controlling the water inside the incubator at 55-60% and controlling the temperature inside the incubator at 28 ℃.
The culture method of the Armillaria mellea branches comprises the following steps:
s1: selecting branches of a fresh oak tree with the diameter of 15cm, cutting the fresh oak tree into small sections of branches with the diameter of 30-50 cm, and cutting two ends of the small sections of branches into inclined planes;
s2: soaking the small sections of branches in 1% urea water solution for 3h, and drying until the water content of the small sections of branches is 40-55%;
s3: placing the branch into a container, adding culture solution, inoculating Armillaria mellea mother strain on the branch, and culturing at 28 deg.C under aseptic condition for 35 days.
The culture solution comprises: 15g of glucose, 1g of monopotassium phosphate, 0.2g of magnesium sulfate, 3mg of zinc sulfate, 5mg of manganese sulfate, 3mg of boric acid, 0.12mg of sodium molybdate, 0.15mg of copper sulfate and 0.05mg of glutamic acid, and adding water to 1 liter.
The first culture material is wood chip powder and straw powder according to the weight ratio of 1: 1 and mixing.
The second culture material layer is formed by mixing corncob particles and perlite according to a weight ratio of 1: 2 and mixing.
The usage amount of the plant growth regulator is 60g/m2。
The plant growth regulator is prepared from gibberellin, cynarin, indolebutyric acid and diethyl aminoethyl hexanoate according to the weight ratio of 1: 1.2: 1.2: 1.2 mixing.
Example 3
A method for soilless culture of gastrodia elata comprises the following steps:
(1) culturing armillaria mellea branches;
(2) preparing cultivation of the osmunda japonica;
(3) and (3) culturing the gastrodia elata: preparing an incubator, paving a first culture material layer at the bottom of the incubator, wherein the thickness of the first culture material layer is 4cm, paving a second culture material layer above the first culture material layer, wherein the thickness of the second culture material layer is 2cm, uniformly mixing germination bacteria and gastrodia elata seeds, paving the mixture above the second culture material layer to form a germination bacteria seed mixed layer, paving the germination bacteria seed mixed layer with the thickness of 0.2cm, uniformly placing armillaria mellea branches above the germination bacteria seed mixed layer, covering the armillaria mellea branches with a coarse sand layer by 2cm, uniformly scattering a plant growth regulator above the coarse sand layer, and then covering with leaves;
(4) and (3) gastrodia elata cultivation management: timely watering is carried out on the incubator, the water inside the incubator is controlled to be 55-60%, and the temperature inside the incubator is controlled to be 22 ℃.
The culture method of the Armillaria mellea branches comprises the following steps:
s1: selecting branches of a fresh oak tree with the diameter of 10cm, cutting the fresh oak tree into small sections of branches with the diameter of 30cm, and cutting two ends of the small sections of branches into inclined planes;
s2: soaking the small sections of branches in 0.6% urea water solution for 1 hour, and drying until the water content of the small sections of branches is 40-55%;
s3: placing the branch into a container, adding culture solution, inoculating Armillaria mellea mother strain on the branch, and culturing at 22 deg.C under aseptic condition for 25 days.
The culture solution comprises: 12g of glucose, 0.5g of monopotassium phosphate, 0.1g of magnesium sulfate, 1mg of zinc sulfate, 2mg of manganese sulfate, 1mg of boric acid, 0.08mg of sodium molybdate, 0.01mg of copper sulfate and 0.01mg of glutamic acid, and adding water to 1 liter.
The first culture material is wood chip powder and straw powder according to the weight ratio of 1: 0.6 mixing.
The second culture material layer is prepared from corncob particles and perlite according to the weight ratio of 1: 1 and mixing.
The usage amount of the plant growth regulator is 40g/m2。
The plant growth regulator is prepared from gibberellin, cynarin, indolebutyric acid and diethyl aminoethyl hexanoate according to the weight ratio of 1: 0.8: 0.8: 0.8 mixing.
Comparative example 1
Comparative example 1 is different from example 1 in that the method for culturing Armillaria mellea does not use a nutrient solution for soaking, and the other steps are the same.
Comparative example 2
Comparative example 2 is different from example 2 in that a culture solution is not used in the Armillaria mellea cultivation method, and the other steps are the same.
Comparative example 3
Comparative example 3 is different from example 1 in that comparative example 3 does not use a plant growth regulator, and the others are the same.
Comparative example 4
Comparative example 4 is different from example 1 in that the plant growth regulator described in comparative example 4 is different from example 1, and the others are the same.
The plant growth regulator is composed of gibberellin and sodium humate according to a weight ratio of 1: 1 and mixing.
To further demonstrate the effect of the present invention, the following test methods were provided:
sowing in 5-7 months, harvesting in 3 months in the next year, and respectively using the embodiments1-3, and comparative examples 1-4, the cultivation area is unified to 50m2After the harvesting, the yield and the gastrodin content in the gastrodia elata are calculated, wherein the gastrodin content is the average value of 5 gastrodia elata, and the measurement results are shown in table 1.
TABLE 1 test results
As can be seen from the table 1, the method for soilless culture of the gastrodia elata can obviously improve the yield of the gastrodia elata and the content of gastrodin in the gastrodia elata, and compared with the examples 1-3, different culture parameters can influence the yield of the gastrodia elata and the content of gastrodin in the gastrodia elata, wherein the example 1 is the optimal parameter; comparing example 1 with comparative examples 1-2, it can be seen that the nutrient solution soaking and culture solution in the armillaria mellea cultivation method can significantly affect the yield of the gastrodia elata and improve the content of gastrodin in the gastrodia elata; comparing example 1 with comparative examples 3 and 4, it can be seen that the selection of the plant growth regulator used in the invention can significantly improve the yield of the gastrodia elata and the content of gastrodin in the gastrodia elata.
In light of the foregoing description of preferred embodiments according to the invention, it is clear that many changes and modifications can be made by the person skilled in the art without departing from the scope of the invention. The technical scope of the present invention is not limited to the contents of the specification, and must be determined according to the scope of the claims.
Claims (7)
1. A method for soilless culture of gastrodia elata is characterized by comprising the following steps:
(1) culturing the armillaria mellea branches;
(2) preparing germination bacteria;
(3) and (3) culturing the gastrodia elata: preparing an incubator, laying a first culture material layer at the bottom of the incubator, wherein the thickness of the first culture material layer is 4-8 cm, laying a second culture material layer above the first culture material layer, wherein the thickness of the second culture material layer is 2-4 cm, uniformly mixing germination bacteria and gastrodia elata seeds, and laying the mixture layer above the second culture material layer to form a germination bacteria seed mixed layer, wherein the laying thickness of the germination bacteria seed mixed layer is 0.2-0.6 cm, uniformly placing armillaria mellea fungus branches above the germination bacteria seed mixed layer, covering the armillaria mellea fungus branches by a coarse sand layer for 2-4 cm, uniformly spreading a plant growth regulator above the coarse sand layer, and then covering by leaves;
(4) and (3) gastrodia elata cultivation management: timely sprinkling water to the incubator, wherein the water content in the incubator is controlled to be 55-60%, and the temperature in the incubator is controlled to be 22-28 ℃;
the culture method of the Armillaria mellea branches comprises the following steps:
s1: selecting branches of a fresh oak tree with the diameter of 10-15 cm, cutting the fresh oak tree into small sections of branches with the diameter of 30-50 cm, and cutting two ends of the small sections of branches into inclined planes;
s2: soaking the small sections of branches in a nutrient solution for 1-3 h, and drying until the water content of the small sections of branches is 40-55%;
s3: putting the small sections of branches into a container, adding a culture solution, inoculating halimasch mother strains on the small sections of branches, and culturing for 25-35 days at the temperature of 22-28 ℃ under an aseptic condition;
the culture solution comprises: 12-15 g of glucose, 0.5-1 g of monopotassium phosphate, 0.1-0.2 g of magnesium sulfate, 1-3 mg of zinc sulfate, 2-5 mg of manganese sulfate, 1-3 mg of boric acid, 0.08-0.12 mg of sodium molybdate, 0.01-0.15 mg of copper sulfate and 0.01-0.05 mg of glutamic acid, and adding water to 1 liter;
the plant growth regulator is prepared from gibberellin, cynarin, indolebutyric acid and diethyl aminoethyl hexanoate according to the weight ratio of 1: 0.8-1.2: 0.8-1.2: 0.8 to 1.2.
2. The method for soilless cultivation of Gastrodia elata as claimed in claim 1, wherein the said nutrient solution is 0.6-1% urea aqueous solution.
3. A method for soilless culture of gastrodia elata as claimed in claim 1, wherein said culture solution includes: 14g of glucose, 0.9g of monopotassium phosphate, 0.15g of magnesium sulfate, 2.8mg of zinc sulfate, 4mg of manganese sulfate, 2.5mg of boric acid, 0.11mg of sodium molybdate, 0.08mg of copper sulfate and 0.04mg of glutamic acid, and water is added to the mixture to reach 1 liter.
4. The method for soilless culture of gastrodia elata as claimed in claim 1, wherein the germination bacteria are Osmunda japonica.
5. The method for soilless culture of gastrodia elata as claimed in claim 1, wherein the first compost is wood flour and straw powder in a weight ratio of 1: 0.6 to 1.
6. The method for soilless culture of gastrodia elata according to claim 1, wherein the second culture material layer is formed by mixing corncob particles and perlite according to a weight ratio of 1: 1-2 mixing.
7. The method for soilless culture of Gastrodia elata as claimed in claim 1, wherein the amount of said plant growth regulator used is 40-60 g/m 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010646850.4A CN111837917B (en) | 2020-07-07 | 2020-07-07 | Method for soilless culture of gastrodia elata |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010646850.4A CN111837917B (en) | 2020-07-07 | 2020-07-07 | Method for soilless culture of gastrodia elata |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111837917A CN111837917A (en) | 2020-10-30 |
| CN111837917B true CN111837917B (en) | 2022-07-05 |
Family
ID=73153673
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010646850.4A Active CN111837917B (en) | 2020-07-07 | 2020-07-07 | Method for soilless culture of gastrodia elata |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111837917B (en) |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103314861B (en) * | 2013-07-08 | 2015-09-23 | 中国科学院华南植物园 | A kind of dendrobium in vitro cross breeding method |
| CN103688751B (en) * | 2013-12-12 | 2016-01-20 | 重庆市福鑫洋食用菌有限公司 | The wooden method making Armillaria mellea of the section of utilization |
| CN105309141A (en) * | 2014-07-14 | 2016-02-10 | 卢鹏 | Taxus chinensis propagating method |
| CN104335820A (en) * | 2014-10-30 | 2015-02-11 | 关岭自治县丰硕食用菌农民专业合作社 | Production method of gastrodia elata associated honey fungus strain |
| CN104611237B (en) * | 2015-01-30 | 2017-11-07 | 麻城市天源中药材种植专业合作社 | A kind of rhizoma Gastrodiae plants cultural method of the symbiosis with halimasch |
| CN107197668B (en) * | 2017-05-27 | 2020-04-28 | 贵州省德均农特产品开发有限公司 | Gastrodia elata planting method |
| CN108184360A (en) * | 2018-02-27 | 2018-06-22 | 潍坊友容实业有限公司 | A kind of method that ecologic planting garden and ecological cultivation system are established in salt-soda soil |
-
2020
- 2020-07-07 CN CN202010646850.4A patent/CN111837917B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN111837917A (en) | 2020-10-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101142889B (en) | High-yield cultivating method for winter jujube in south | |
| CN104186215B (en) | A kind of breeding method of large tea oil tree potted landscape | |
| CN104737782A (en) | Planting method for south ramulus mori bamboo fungus | |
| CN107567926A (en) | A kind of fig and asparagus interplanting method | |
| CN106718539A (en) | A kind of mulberry tree implantation methods | |
| CN105993605A (en) | High-yield cultivation technology for polyporus umbellatus | |
| CN106386375A (en) | Grafting cultivation method for vitis quinquangularis | |
| CN105724166B (en) | A kind of female climing result of greenhouse controlling list two ripe cultural method year after year | |
| CN104429791A (en) | Grape planting method | |
| CN109247178B (en) | Method for improving planting survival rate of mercerizing wood in saline-alkali soil | |
| CN107047183A (en) | A kind of longan implantation methods | |
| CN101803555B (en) | Wax apple nutrient solution culture method | |
| CN108377830B (en) | Method for cultivating clump-growing type large seedlings of red flower Tianmu | |
| CN111837917B (en) | Method for soilless culture of gastrodia elata | |
| CN110720378A (en) | Special matrix for melastoma herb and red peony plants and application thereof | |
| CN110338064B (en) | Rooting culture and seedling raising method for ulmus davidiana tissue culture seedlings | |
| CN114679999A (en) | Arbuscular mycorrhizal fungi microbial inoculum based on corn and preparation method thereof | |
| CN112262721A (en) | Selenium-rich radix puerariae planting method | |
| CN105900680A (en) | Rose flower seedling transplanting technology | |
| CN107360922B (en) | Cultivation method of taxus mairei | |
| CN112136630A (en) | High-yield planting method for red-core sweet potatoes | |
| CN105613239B (en) | The preparation method and balcony horticultural gardening matrix of balcony horticultural gardening matrix | |
| CN110089393A (en) | A kind of seeds of Gastrodia elata nursery nutrient matrix | |
| CN104956866B (en) | A kind of black pearl cherry and interplanting corn method | |
| CN108934506A (en) | Organic grape green inoculation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: Building F2, 39 Ruihe Road, Huangpu District, Guangzhou City, Guangdong Province 510000 Applicant after: Lancy Purcell Biotechnology (Guangzhou) Co.,Ltd. Address before: 510000 Room D09, 4th Floor, 131 Airport Road, Baiyun District, Guangzhou City, Guangdong Province Applicant before: Lancy Purcell Biotechnology (Guangzhou) Co.,Ltd. |
|
| CB02 | Change of applicant information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A Method for Soilless Cultivation of Gastrodia elata Effective date of registration: 20230629 Granted publication date: 20220705 Pledgee: Hunan Pingjiang Rural Commercial Bank Co.,Ltd. Pledgor: Chen Zhongping|Lancy Purcell Biotechnology (Guangzhou) Co.,Ltd. Registration number: Y2023980046730 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |