CN111826331A - Microbial beneficial live bacterium agent for preventing fat-raised mutton-sheep yellow fat sheep and preparation method thereof - Google Patents
Microbial beneficial live bacterium agent for preventing fat-raised mutton-sheep yellow fat sheep and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a microorganism beneficial live bacterium agent for preventing fattening mutton sheep from becoming yellow fat sheep and a preparation method thereof. The microorganism beneficial live bacteria agent comprises the following raw materials in parts by weight: 2-4 parts of bacillus, 1-3 parts of lactic acid bacteria, 2-4 parts of saccharomycetes, 1-3 parts of light and bacteria, 8-12 parts of culture medium and 60-100 parts of well water. The raw materials required by the microbial beneficial live bacteria agent for preventing fattening mutton sheep from becoming yellow fat sheep comprise bacillus, lactic acid bacteria, saccharomycetes, light and bacteria, a culture medium, well water and the like, are wide in source and low in price, and can be prepared, so that the cost of farmers is greatly reduced. Moreover, the beneficial microorganism live bacteria agent only needs to be added into the feed, so that the yellow fat is effectively prevented, the morbidity is reduced, and the economic loss of farmers is greatly reduced.
Description
Technical Field
The invention belongs to the technical field of animal nutrition and feed science, and particularly relates to a microbial beneficial live bacterium agent for preventing fattening mutton sheep yellow fat sheep and a preparation method thereof.
Background
With the improvement of the living standard of human beings, the demand of meat is large. Recently, due to the fact that African swine fever has been abused, the market demand for mutton is larger and larger, the demand for mutton is short in recent years as the price of mutton is high, and farmers start to breed sheep in a large scale with great support of governments, but breeding problems are endless.
The sheep yellow fat is a common disease of modern sheep, and the yellow fat is a pigment deposition disease characterized by yellow fat tissue of sheep, also called yellow fat. In order to enable the sheep to grow faster, farmers feed a large amount of concentrated feed to cause the sheep to obtain a large amount of fat sheep, and the sheep cannot be sold after the fat sheep is obtained. Once the disease occurs, the cure rate is extremely low, and great economic loss is brought to mutton sheep farmers.
Therefore, the invention aims to provide a method for solving the problem of low economic cost of the yellow fat sheep, so that the burden of farmers is not increased, and the breeding income can be improved.
Disclosure of Invention
The invention aims to solve the technical problems and provides a microbial beneficial live bacterium agent for preventing fat-raised mutton sheep from becoming yellow fat and a preparation method thereof
In order to solve the problems, the invention is realized according to the following technical scheme:
the invention relates to a microbial beneficial live bacterium agent for preventing fattening mutton sheep yellow fat sheep, which comprises the following raw materials in parts by weight:
2-4 parts of bacillus, 1-3 parts of lactic acid bacteria, 2-4 parts of saccharomycetes, 1-3 parts of light and bacteria, 8-12 parts of culture medium and 60-100 parts of well water.
Preferably, the microorganism beneficial live agent comprises the following raw materials in parts by weight:
3 parts of bacillus, 2 parts of lactic acid bacteria, 3 parts of saccharomycetes, 2 parts of light and bacteria, 10 parts of culture medium and 80 parts of well water.
Preferably, the microbial beneficial live agent is prepared by a preparation method comprising the following steps:
(1) putting well water into a container, heating to 45 ℃, putting a culture medium, bacillus, lactic acid bacteria, saccharomycetes, light and bacteria into the container in sequence, and fully and uniformly stirring to obtain a compound bacterial liquid;
(2) standing and fermenting the mixed solution for 24 hours in a constant temperature environment, wherein the temperature of the constant temperature environment is 30 ℃, and the fermented composite bacteria liquid is obtained to obtain composite bacteria mother liquor;
(3) adding brown sugar into the composite bacterium mother liquor, mixing, dividing the composite bacterium mother liquor into a plurality of sub-liquors in equal parts, and carrying out amplification propagation on the sub-liquors to 10 times to obtain the beneficial microbial viable bacteria agent.
Preferably, the microbial beneficial live bacteria further comprise:
(4) the beneficial microorganism live bacteria agent is added into the feed according to the weight proportion of 0.4 percent to prepare the beneficial microorganism live bacteria agent feed for preventing fat-raised mutton sheep and sheep with yellow fat.
The invention also provides a preparation method of the microbial beneficial live bacteria agent for preventing fattening mutton sheep with yellow fat, which comprises the following steps:
(1) putting well water into a container, heating to 45 ℃, putting a culture medium, bacillus, lactic acid bacteria, saccharomycetes, light and bacteria into the container in sequence, and fully and uniformly stirring to obtain a compound bacterial liquid;
the raw materials are as follows by weight: 2-4 parts of bacillus, 1-3 parts of lactic acid bacteria, 2-4 parts of saccharomycetes, 1-3 parts of photosynthetic bacteria, 8-12 parts of culture medium and 60-100 parts of well water;
(2) standing and fermenting the mixed solution for 24 hours in a constant temperature environment, wherein the temperature of the constant temperature environment is 30 ℃, and the fermented composite bacteria liquid is obtained to obtain composite bacteria mother liquor;
(3) adding brown sugar into the composite bacterium mother liquor, mixing, dividing the composite bacterium mother liquor into a plurality of sub-liquors in equal parts, and carrying out amplification propagation on the sub-liquors to 10 times to obtain the beneficial microbial viable bacteria agent.
Preferably, the bacillus 3 parts, lactobacillus 2 parts, yeast 3 parts, light and bacteria 2 parts, culture medium 10 parts and well water 80 parts.
Preferably, the preparation method further comprises:
(4) the beneficial microorganism live bacteria agent is added into the feed according to the weight proportion of 0.4 percent to prepare the beneficial microorganism live bacteria agent feed for preventing fat-raised mutton sheep and sheep with yellow fat.
Compared with the prior art, the invention has the beneficial effects that:
the raw materials required by the microbial beneficial live bacteria agent for preventing fattening mutton sheep from becoming yellow fat sheep comprise bacillus, lactic acid bacteria, saccharomycetes, light and bacteria, a culture medium, well water and the like, are wide in source, low in price and simple in preparation process, and the cost of farmers is greatly reduced. Moreover, the beneficial microorganism live bacteria agent only needs to be added into the feed, so that the yellow fat is effectively prevented, the morbidity is reduced, and the economic loss of farmers is greatly reduced.
Detailed Description
The following description of the preferred embodiments of the present invention is provided for the purpose of illustration and description, and is in no way intended to limit the invention.
The sheep yellow fat is a pigmentation disease which is characterized in that fat tissues of sheep are yellow, and is also called as yellow fat, and through a great deal of research and research by the applicant, the main reasons for causing the yellow fat in the existing breeding comprise:
yellow fat caused by feed
(1) In order to make sheep grow faster, farmers feed a large amount of concentrated feed. The high-energy and high-protein feed is fed to destroy the growth rule of sheep, the sheep is a grazing animal, the house-fed mutton sheep fattening is morbid fattening, and in order to achieve the purpose of quick growth of the sheep, the concentrated feed is large in feeding amount, excessive nutrition for a long time causes in-vivo metabolism abnormality, the occurrence of fatty liver and liver function abnormality causes bile secretion and metabolism abnormality, biliary tract blockage, and the bile is directly absorbed into blood to cause jaundice meat.
(2) In order to reduce cost, farmers do not use the feed according to the recommended proportion of manufacturers during feed preparation, so that the feed proportion is unreasonable and the nutrition collocation is unbalanced. The addition of more kinds of auxiliary materials and the small proportion of the added concentrated materials accelerate the vitamin deficiency, other various nutrient components are difficult to ensure and balance, and the lack of forage grass supply for quality and quantity preservation leads to the occurrence of the yellow fat.
Therefore, the application provides the microbial beneficial live bacteria for preventing the yellow fat sheep of the fattening sheep, which can be added into the feed. Through the compound probiotics, various enzymes generated by the compound probiotics are utilized to decompose the substances which are not digestible and absorbable by sheep in the concentrated feed, and the like, so that the yellow fat caused by the influence of the feed is reduced, and the aim of preventing the yellow fat sheep is fulfilled.
In a first aspect, the invention provides a microbial beneficial live bacterium agent for preventing fattening mutton sheep from becoming yellow fat sheep, which is added into feed, and the microbial beneficial live bacterium agent comprises the following raw materials in parts by weight: 2-4 parts of bacillus, 1-3 parts of lactic acid bacteria, 2-4 parts of saccharomycetes, 1-3 parts of light and bacteria, 8-12 parts of culture medium and 60-100 parts of well water.
The applicant finds that the pathogenesis of the yellow fat sheep is mainly as follows through deep research:
1. the feed has high content of grain substances. The bacillus produces enzymes (pectinase, amylase, protease, cellulase and the like), and various enzyme preparations can promote the digestion and absorption of parts which are not digested and absorbed in the feed and prevent the yellow fat.
2. Vitamin E deficiency. The vitamin E as an antioxidant can prevent or delay the self-oxidation of unsaturated fatty acid, promote fat cells to convert the unsaturated fatty acid into storage fat, when the vitamin E is seriously deficient, the oxidation of the unsaturated fatty acid enhances the deposition of wax-like substances in fat tissues to be accelerated, and the fat tissues generate inflammatory reaction to cause the fat to turn yellow so as to generate the fat lining. The invention adopts lactic acid bacteria, which can generate a large amount of vitamins and supplement vitamin E to prevent the yellow fat.
3. And removing the aspergillus flavus. Farmers can purchase and store a large amount of feed in advance, and the feed is easy to mildew to generate aspergillus flavus due to improper feed management. When the sheep were fed with the feed infected with Aspergillus flavus, these were all factors responsible for this disease. Therefore, the invention also separates a plurality of antibacterial substances through lactic acid bacteria, and the effects of adhering and degrading the aflatoxin are very obvious. The synergistic yeast has degradation and removal effects on aflatoxin.
In the present invention, the light and bacteria are also called photosynthetic bacteria. The light and bacteria produce multiple carotenoids, which are natural antioxidant nutrients, and the carotenoids have a mutual coordination effect with vitamin E, so that the deposition of acid-resistant pigments in fat tissues is avoided, and the prevention of the fat obesity is realized. In the invention, drinking water wells are generally arranged in farmers and are natural water, and the well water is mainly used as a solvent and used for mixing various raw materials. The well water contains less chemical agents such as disinfectants and the like, and is more suitable for feeding.
In the present invention, the medium is used to provide nutrition to various probiotics, and the selective use of the medium is known to those skilled in the art and is not described herein too much.
In a second aspect, the invention provides a preparation method of a microbial beneficial live bacterium agent for preventing fattening mutton sheep yellow fat sheep, which comprises the following steps:
(1) putting well water into a container, heating to 45 ℃, putting a culture medium, bacillus, lactic acid bacteria, saccharomycetes, light and bacteria into the container in sequence, and fully and uniformly stirring to obtain a compound bacterial liquid;
wherein, the bacillus comprises 2-4 parts of bacillus, 1-3 parts of lactobacillus, 2-4 parts of saccharomycetes, 1-3 parts of photosynthetic bacteria, 8-12 parts of culture medium and 60-100 parts of well water;
(2) standing and fermenting the mixed solution for 24 hours in a constant temperature environment, wherein the temperature of the constant temperature environment is 30 ℃, and the fermented composite bacteria liquid is obtained to obtain composite bacteria mother liquor;
(3) adding brown sugar into the composite bacterium mother liquor, mixing, dividing the composite bacterium mother liquor into a plurality of sub-liquors in equal parts, and carrying out amplification propagation on the sub-liquors to 10 times to obtain the beneficial microbial viable bacteria agent.
(4) The beneficial microorganism live bacteria agent is added into the feed according to the weight proportion of 0.4 percent to prepare the beneficial microorganism live bacteria agent feed for preventing fat-raised mutton sheep and sheep with yellow fat.
Specifically, the microorganism beneficial live bacteria agent is added into the feed according to 0.4 percent of the weight of the feed. Preferably, the content of copper element in the feed is 50-100 mg/kg. The research of the applicant finds that the excessive trace element copper is also one of the elements inducing the lamb fat.
In the above preparation method, the feed may be a general feed or a concentrated feed. The compound probiotics such as bacillus is utilized to generate various enzymes to decompose the substances which are not digestible and absorbable by sheep in the concentrated feed, thereby achieving the purpose of preventing the cattle with yellow fat.
Example 1
The beneficial microorganism live bacterium agent for preventing fattening mutton sheep yellow fat sheep in the embodiment 1 is added into feed, and comprises the following raw materials in parts by weight: 2 parts of bacillus, 1 part of lactic acid bacteria, 2 parts of saccharomycetes, 1 part of light and bacteria, 8 parts of culture medium and 60 parts of well water.
The preparation method of the microbial beneficial live bacteria agent for preventing fattening mutton sheep yellow fat sheep in the embodiment 1 comprises the following steps:
(1) putting well water into a container, heating to 45 ℃, putting the culture medium, the bacillus, the lactic acid bacteria, the saccharomycetes, the light and the bacteria in parts by weight into the container in sequence, and fully and uniformly stirring to obtain a compound bacterial liquid;
(2) standing and fermenting the mixed solution for 24 hours in a constant temperature environment, wherein the temperature of the constant temperature environment is 30 ℃, and the fermented composite bacteria liquid is obtained to obtain composite bacteria mother liquor;
(3) adding brown sugar into the composite bacterium mother liquor, mixing, dividing the composite bacterium mother liquor into a plurality of sub-liquors in equal parts, and carrying out amplification propagation on the sub-liquors to 10 times to obtain the beneficial microbial viable bacteria agent.
(4) The beneficial microorganism live bacteria agent is added into the feed according to the weight proportion of 0.4 percent to prepare the beneficial microorganism live bacteria agent feed for preventing fat-raised mutton sheep and sheep with yellow fat.
Example 2
The microorganism-beneficial live bacteria agent for preventing fattening sheep yellow fat sheep and the preparation method of the microorganism-beneficial live bacteria agent are the same as those in example 1. The difference is that the raw materials with different parts by weight are adopted, and the raw materials comprise:
3 parts of bacillus, 2 parts of lactic acid bacteria, 3 parts of saccharomycetes, 2 parts of light and bacteria, 10 parts of culture medium and 80 parts of well water.
Example 3
The microorganism-beneficial live bacteria agent for preventing fattening sheep yellow fat sheep and the preparation method of the microorganism-beneficial live bacteria agent are the same as those in example 1. The difference is that the raw materials with different parts by weight are adopted, and the raw materials comprise:
5 parts of bacillus, 3 parts of lactic acid bacteria, 5 parts of saccharomycetes, 3 parts of light and bacteria, 12 parts of culture medium and 100 parts of well water.
Test verification
In the partner's farm, the experiment selected the chender jiaojiao goats introduced from north of river: 100 male and female animals (the proportion is 6:4, the weight is 26.8-28.0 kg, the health is free from diseases, the age is 3 months old) are randomly divided into two groups, including a control group and a test group.
Wherein the control group is fed with conventional feed. The test groups are fed with the microbial beneficial live bacteria agent feed for preventing fat-fatted mutton sheep and sheep yellow fat sheep, which is prepared in the embodiment 2 of the invention. The control group and the test group used the same feed. The feeding level and the preparation of the two groups of sheep are consistent, and the ratio of the concentrated feed to the coarse feed is 80: 20. The test period is 90 days, the feed is fed for 3 times a day, the time is 8:00, 12:00 and 17:00, and water is freely drunk.
According to the sheep farm immunization system, the test sheep are subjected to immune injection, and the colony house is thoroughly disinfected once. After the test period, the test sheep were slaughtered to observe and record the condition of the sheep yellow fat. The basic conditions of the test sheep and the test results are as follows:
| group of | Initial weight (kg) | Terminal weight (kg) | Number of yellow fat appeared (only) |
| Test group | 26.8±1.1 | 65.1±2.1 | 2 |
| Control group | 27±1.05 | 64.2±4.09 | 16 |
In the control group, the number of yellow fat appeared (only) was 16. In the test group, the number of yellow fat appeared (only) was 2.
Results and analysis: after 3 months of tracking observation, after the test group adds the microorganism beneficial live bacteria agent for preventing the fattening sheep from becoming yellow fat into the feed, 2 of the test group 50 have become yellow fat, 16 of the control group 50 have become yellow fat, and the incidence of the yellow fat of the test group is obviously lower than that of the control group.
And (4) conclusion: the invention can obviously prevent the occurrence of the disease condition of fattening sheep with fat meat and mutton, namely the yellow fat sheep.
The effect is as follows: in summary, the composite probiotic preparation is creatively prepared by only adopting four probiotics and is mixed with feed according to a specific proportion, so that the good effect of preventing the yellow fat can be achieved. The required raw materials comprise bacillus, lactic acid bacteria, saccharomycetes, light and bacteria, a culture medium, well water and the like, the sources are wide, the price is low, the preparation process is simple, and the cost of farmers is greatly reduced. Moreover, the beneficial microorganism live bacteria agent only needs to be added into the feed, so that the yellow fat is effectively prevented, the morbidity is reduced, and the economic loss of farmers is greatly reduced.
Example 4
The microorganism-beneficial live bacteria agent for preventing fattening sheep yellow fat sheep and the preparation method of the microorganism-beneficial live bacteria agent are the same as those in example 1. The difference is that raw materials with different weight parts are adopted, and the raw materials comprise selenium polysaccharide.
(1) Putting well water into a container, heating to 45 ℃, putting the culture medium, the bacillus, the lactic acid bacteria, the saccharomycetes, the light and the bacteria in parts by weight into the container in sequence, and fully and uniformly stirring to obtain a compound bacterial liquid;
(2) standing and fermenting the mixed solution for 24 hours in a constant temperature environment, wherein the temperature of the constant temperature environment is 30 ℃, and the fermented composite bacteria liquid is obtained to obtain composite bacteria mother liquor;
(3) adding brown sugar into the composite bacterium mother liquor, mixing, dividing the composite bacterium mother liquor into a plurality of seed liquors in one part, carrying out amplification propagation on the seed liquors to 10 times, adding yeast into the seed liquors after amplification propagation according to the ratio of 60-90 mg/kg, drying in the sun, and mixing to obtain the beneficial microorganism viable bacteria agent;
in specific embodiments, 80mg/kg yeast is added, sun dried and mixed.
(4) The beneficial microorganism live bacteria agent is added into the feed according to the weight proportion of 0.4 percent to prepare the beneficial microorganism live bacteria agent feed for preventing fat-raised mutton sheep and sheep with yellow fat.
The research of the applicant finds that the yeast is added for drying, the pathogeny that fat is turned yellow to generate the yellow fat due to the fact that the deposition of wax-like substances is accelerated in adipose tissues because unsaturated fatty acid is oxidized and enhanced and the inflammatory reaction of the adipose tissues is generated is solved, and the disease of the fat-raised mutton and mutton yellow fat sheep is effectively reduced. And the yeast sun-drying also has the advantages of low price and reduction of the cost of farmers.
Test verification
In the partner's farm, the experiment selected the chender jiaojiao goats introduced from north of river: 100 male and female (the proportion is 6:4, the weight is 26.2-29.0 kg, the health is free from diseases, and the life is 3 months old).
The microbial beneficial live bacterium agent feed for preventing fat-fatted mutton sheep prepared in the embodiment 4 of the invention is added. (the feed storage is standard and reasonable, and the mildew does not exist), and the proportion of the fine and coarse feed is 80: 20. The test period is 90 days, the feed is fed for 3 times a day, the time is 8:00, 12:00 and 17:00, and water is freely drunk.
According to the sheep farm immunization system, the test sheep are subjected to immune injection, and the colony house is thoroughly disinfected once. After the test period, the test sheep were slaughtered to observe and record the condition of the sheep yellow fat. The basic conditions of the test sheep and the test results are as follows:
| group of | Initial weight (kg) | Terminal weight (kg) | Number of yellow fat appeared (only) |
| Test group | 27.2±1.1 | 65.1±4.1 | 0 |
And (4) conclusion: after the tracking observation for 3 months, the test group does not have the condition of the yellow fat after the microorganism beneficial live bacteria agent for preventing the fattening sheep yellow fat sheep in the example 4 is added into the feed.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.
Claims (7)
1. The microbial beneficial live agent for preventing fattening mutton sheep yellow fat sheep is characterized by comprising the following raw materials in parts by weight:
2-4 parts of bacillus, 1-3 parts of lactic acid bacteria, 2-4 parts of saccharomycetes, 1-3 parts of light and bacteria, 8-12 parts of culture medium and 60-100 parts of well water.
2. The microbial beneficial live agent for preventing fattening mutton sheep yellow fat sheep as claimed in claim 1, which is characterized by comprising the following raw materials in parts by weight:
3 parts of bacillus, 2 parts of lactic acid bacteria, 3 parts of saccharomycetes, 2 parts of light and bacteria, 10 parts of culture medium and 80 parts of well water.
3. The live microbial benefit agent for preventing fattening sheep yellow fat sheep according to claim 1 or 2, wherein the live microbial benefit agent is prepared by the following preparation method, and the preparation method comprises the following steps:
(1) putting well water into a container, heating to 45 ℃, putting a culture medium, bacillus, lactic acid bacteria, saccharomycetes, light and bacteria into the container in sequence, and fully and uniformly stirring to obtain a compound bacterial liquid;
(2) standing and fermenting the mixed solution for 24 hours in a constant temperature environment, wherein the temperature of the constant temperature environment is 30 ℃, and the fermented composite bacteria liquid is obtained to obtain composite bacteria mother liquor;
(3) adding brown sugar into the composite bacterium mother liquor, mixing, dividing the composite bacterium mother liquor into a plurality of sub-liquors in equal parts, and carrying out amplification propagation on the sub-liquors to 10 times to obtain the beneficial microbial viable bacteria agent.
4. The live microbial benefit agent for preventing fattening sheep yellow fat sheep according to claim 3, further comprising:
(4) the beneficial microorganism live bacteria agent is added into the feed according to the weight proportion of 0.4 percent to prepare the beneficial microorganism live bacteria agent feed for preventing fat-raised mutton sheep and sheep with yellow fat.
5. A preparation method of a microorganism beneficial live bacteria agent for preventing fattening mutton sheep yellow fat sheep, which is characterized by comprising the following steps:
(1) putting well water into a container, heating to 45 ℃, putting a culture medium, bacillus, lactic acid bacteria, saccharomycetes, light and bacteria into the container in sequence, and fully and uniformly stirring to obtain a compound bacterial liquid;
the raw materials are as follows by weight: 2-4 parts of bacillus, 1-3 parts of lactic acid bacteria, 2-4 parts of saccharomycetes, 1-3 parts of photosynthetic bacteria, 8-12 parts of culture medium and 60-100 parts of well water;
(2) standing and fermenting the mixed solution for 24 hours in a constant temperature environment, wherein the temperature of the constant temperature environment is 30 ℃, and the fermented composite bacteria liquid is obtained to obtain composite bacteria mother liquor;
(3) adding brown sugar into the composite bacterium mother liquor, mixing, dividing the composite bacterium mother liquor into a plurality of sub-liquors in equal parts, and carrying out amplification propagation on the sub-liquors to 10 times to obtain the beneficial microbial viable bacteria agent.
6. The method for preparing a microbial beneficial agent for preventing fattening sheep yellow fat sheep according to claim 5, wherein the method comprises the following steps:
3 parts of bacillus, 2 parts of lactic acid bacteria, 3 parts of saccharomycetes, 2 parts of light and bacteria, 10 parts of culture medium and 80 parts of well water.
7. The method for preparing a viable microorganism-beneficial agent for preventing fattening sheep yellow fat sheep according to claim 5, wherein the preparation method further comprises:
(4) the beneficial microorganism live bacteria agent is added into the feed according to the weight proportion of 0.4 percent to prepare the beneficial microorganism live bacteria agent feed for preventing fat-raised mutton sheep and sheep with yellow fat.
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| 罗成贵: "猪黄膘肉形成原因、鉴定处理及预防措施" * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114521885A (en) * | 2022-02-22 | 2022-05-24 | 吉林农业大学 | Limb positioning type body fat detection structure realized by using bioelectrical impedance principle |
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