CN111821209B - 一种复配型天然美白抗皱组合物和应用 - Google Patents
一种复配型天然美白抗皱组合物和应用 Download PDFInfo
- Publication number
- CN111821209B CN111821209B CN202010844858.1A CN202010844858A CN111821209B CN 111821209 B CN111821209 B CN 111821209B CN 202010844858 A CN202010844858 A CN 202010844858A CN 111821209 B CN111821209 B CN 111821209B
- Authority
- CN
- China
- Prior art keywords
- pterostilbene
- whitening
- procyanidine
- skin
- wrinkle composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000002087 whitening effect Effects 0.000 title claims abstract description 35
- 239000000203 mixture Substances 0.000 title claims abstract description 24
- 230000001153 anti-wrinkle effect Effects 0.000 title claims abstract description 23
- 150000001875 compounds Chemical class 0.000 title claims abstract description 9
- VLEUZFDZJKSGMX-UHFFFAOYSA-N pterostilbene Natural products COC1=CC(OC)=CC(C=CC=2C=CC(O)=CC=2)=C1 VLEUZFDZJKSGMX-UHFFFAOYSA-N 0.000 claims abstract description 37
- VLEUZFDZJKSGMX-ONEGZZNKSA-N pterostilbene Chemical compound COC1=CC(OC)=CC(\C=C\C=2C=CC(O)=CC=2)=C1 VLEUZFDZJKSGMX-ONEGZZNKSA-N 0.000 claims abstract description 37
- 230000032683 aging Effects 0.000 claims abstract description 8
- 238000002360 preparation method Methods 0.000 claims abstract description 5
- 239000004480 active ingredient Substances 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 abstract description 5
- 230000007794 irritation Effects 0.000 abstract description 4
- 208000003351 Melanosis Diseases 0.000 abstract description 3
- 238000005562 fading Methods 0.000 abstract description 3
- 206010020751 Hypersensitivity Diseases 0.000 abstract description 2
- 208000026935 allergic disease Diseases 0.000 abstract description 2
- 230000007815 allergy Effects 0.000 abstract description 2
- 230000000704 physical effect Effects 0.000 abstract description 2
- 230000005764 inhibitory process Effects 0.000 description 24
- 102000003425 Tyrosinase Human genes 0.000 description 21
- 108060008724 Tyrosinase Proteins 0.000 description 21
- 230000000694 effects Effects 0.000 description 20
- XFZJEEAOWLFHDH-NFJBMHMQSA-N procyanidin B2 Chemical compound C1([C@@H]2[C@H](O)[C@H](C3=C(O)C=C(O)C=C3O2)C=2C(O)=CC(O)=C3C[C@H]([C@H](OC3=2)C=2C=C(O)C(O)=CC=2)O)=CC=C(O)C(O)=C1 XFZJEEAOWLFHDH-NFJBMHMQSA-N 0.000 description 14
- 102000016387 Pancreatic elastase Human genes 0.000 description 13
- 108010067372 Pancreatic elastase Proteins 0.000 description 13
- 241000196324 Embryophyta Species 0.000 description 11
- 229920002350 Procyanidin B2 Polymers 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- XFZJEEAOWLFHDH-UHFFFAOYSA-N (2R,2'R,3R,3'R,4R)-3,3',4',5,7-Pentahydroxyflavan(48)-3,3',4',5,7-pentahydroxyflavan Natural products C=12OC(C=3C=C(O)C(O)=CC=3)C(O)CC2=C(O)C=C(O)C=1C(C1=C(O)C=C(O)C=C1O1)C(O)C1C1=CC=C(O)C(O)=C1 XFZJEEAOWLFHDH-UHFFFAOYSA-N 0.000 description 7
- 230000002195 synergetic effect Effects 0.000 description 7
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
- 230000037303 wrinkles Effects 0.000 description 6
- WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 description 5
- WTDRDQBEARUVNC-UHFFFAOYSA-N L-Dopa Natural products OC(=O)C(N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-UHFFFAOYSA-N 0.000 description 5
- CWEZAWNPTYBADX-UHFFFAOYSA-N Procyanidin Natural products OC1C(OC2C(O)C(Oc3c2c(O)cc(O)c3C4C(O)C(Oc5cc(O)cc(O)c45)c6ccc(O)c(O)c6)c7ccc(O)c(O)c7)c8c(O)cc(O)cc8OC1c9ccc(O)c(O)c9 CWEZAWNPTYBADX-UHFFFAOYSA-N 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 5
- 229920002414 procyanidin Polymers 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- AHMIDUVKSGCHAU-UHFFFAOYSA-N Dopaquinone Natural products OC(=O)C(N)CC1=CC(=O)C(=O)C=C1 AHMIDUVKSGCHAU-UHFFFAOYSA-N 0.000 description 4
- AHMIDUVKSGCHAU-LURJTMIESA-N L-dopaquinone Chemical compound [O-]C(=O)[C@@H]([NH3+])CC1=CC(=O)C(=O)C=C1 AHMIDUVKSGCHAU-LURJTMIESA-N 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 4
- 239000002537 cosmetic Substances 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 4
- 229910021641 deionized water Inorganic materials 0.000 description 4
- 239000000839 emulsion Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 230000037394 skin elasticity Effects 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 239000003963 antioxidant agent Substances 0.000 description 3
- 235000006708 antioxidants Nutrition 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 150000003254 radicals Chemical class 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 2
- 102000016942 Elastin Human genes 0.000 description 2
- 108010014258 Elastin Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
- 230000000172 allergic effect Effects 0.000 description 2
- 230000003712 anti-aging effect Effects 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 208000010668 atopic eczema Diseases 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 229920002549 elastin Polymers 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- WZNJWVWKTVETCG-UHFFFAOYSA-N kojic acid Natural products OC(=O)C(N)CN1C=CC(=O)C(O)=C1 WZNJWVWKTVETCG-UHFFFAOYSA-N 0.000 description 2
- BEJNERDRQOWKJM-UHFFFAOYSA-N kojic acid Chemical compound OCC1=CC(=O)C(O)=CO1 BEJNERDRQOWKJM-UHFFFAOYSA-N 0.000 description 2
- 229960004705 kojic acid Drugs 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229930014626 natural product Natural products 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 239000008055 phosphate buffer solution Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- PFTAWBLQPZVEMU-ZFWWWQNUSA-N (+)-epicatechin Natural products C1([C@@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-ZFWWWQNUSA-N 0.000 description 1
- PFTAWBLQPZVEMU-UKRRQHHQSA-N (-)-epicatechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-UKRRQHHQSA-N 0.000 description 1
- LSHVYAFMTMFKBA-TZIWHRDSSA-N (-)-epicatechin-3-O-gallate Chemical compound O([C@@H]1CC2=C(O)C=C(C=C2O[C@@H]1C=1C=C(O)C(O)=CC=1)O)C(=O)C1=CC(O)=C(O)C(O)=C1 LSHVYAFMTMFKBA-TZIWHRDSSA-N 0.000 description 1
- MJYQFWSXKFLTAY-OVEQLNGDSA-N (2r,3r)-2,3-bis[(4-hydroxy-3-methoxyphenyl)methyl]butane-1,4-diol;(2r,3r,4s,5s,6r)-6-(hydroxymethyl)oxane-2,3,4,5-tetrol Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O.C1=C(O)C(OC)=CC(C[C@@H](CO)[C@H](CO)CC=2C=C(OC)C(O)=CC=2)=C1 MJYQFWSXKFLTAY-OVEQLNGDSA-N 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 244000301850 Cupressus sempervirens Species 0.000 description 1
- 241000371652 Curvularia clavata Species 0.000 description 1
- LSHVYAFMTMFKBA-UHFFFAOYSA-N ECG Natural products C=1C=C(O)C(O)=CC=1C1OC2=CC(O)=CC(O)=C2CC1OC(=O)C1=CC(O)=C(O)C(O)=C1 LSHVYAFMTMFKBA-UHFFFAOYSA-N 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 235000011201 Ginkgo Nutrition 0.000 description 1
- 244000194101 Ginkgo biloba Species 0.000 description 1
- 235000008100 Ginkgo biloba Nutrition 0.000 description 1
- 229920000161 Locust bean gum Polymers 0.000 description 1
- 241000169546 Lycium ruthenicum Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- 241000750718 Pterocarpus santalinus Species 0.000 description 1
- 235000004789 Rosa xanthina Nutrition 0.000 description 1
- 241000109329 Rosa xanthina Species 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 206010040880 Skin irritation Diseases 0.000 description 1
- 206010042674 Swelling Diseases 0.000 description 1
- LUKBXSAWLPMMSZ-OWOJBTEDSA-N Trans-resveratrol Chemical class C1=CC(O)=CC=C1\C=C\C1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-OWOJBTEDSA-N 0.000 description 1
- 235000003095 Vaccinium corymbosum Nutrition 0.000 description 1
- 240000000851 Vaccinium corymbosum Species 0.000 description 1
- 235000017537 Vaccinium myrtillus Nutrition 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 206010000496 acne Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003679 aging effect Effects 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 229940093797 bioflavonoids Drugs 0.000 description 1
- 235000021014 blueberries Nutrition 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003602 elastase inhibitor Substances 0.000 description 1
- LPTRNLNOHUVQMS-UHFFFAOYSA-N epicatechin Natural products Cc1cc(O)cc2OC(C(O)Cc12)c1ccc(O)c(O)c1 LPTRNLNOHUVQMS-UHFFFAOYSA-N 0.000 description 1
- 235000012734 epicatechin Nutrition 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 102000034240 fibrous proteins Human genes 0.000 description 1
- 108091005899 fibrous proteins Proteins 0.000 description 1
- 235000004426 flaxseed Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000000245 forearm Anatomy 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
- ACCCMOQWYVYDOT-UHFFFAOYSA-N hexane-1,1-diol Chemical compound CCCCCC(O)O ACCCMOQWYVYDOT-UHFFFAOYSA-N 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000008099 melanin synthesis Effects 0.000 description 1
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 description 1
- -1 methoxysuccinyl Chemical group 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 230000037311 normal skin Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- UWJJYHHHVWZFEP-UHFFFAOYSA-N pentane-1,1-diol Chemical compound CCCCC(O)O UWJJYHHHVWZFEP-UHFFFAOYSA-N 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 235000010204 pine bark Nutrition 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000012088 reference solution Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 230000036556 skin irritation Effects 0.000 description 1
- 231100000475 skin irritation Toxicity 0.000 description 1
- 230000037339 smooth wrinkles Effects 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/347—Phenols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dermatology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Emergency Medicine (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Botany (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Cosmetics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
本发明公开了一种复配型天然美白抗皱组合物和应用。它含有原花青素B2和紫檀芪作为活性成分。本发明方法得到的天然植物源美白抗皱组合物性质稳定，应用于护肤产品中无刺激、抗过敏、美白淡斑、抗衰老。本发明制备方法简单，功效显著，具有很好的应用前景。
Description
技术领域
本发明涉及化妆品技术领域，具体涉及一种复配型美白抗皱组合物和在美白、抗皱等护肤品、保健品中的应用。
背景技术
自由基是机体氧化反应中产生的有害化合物，有强氧化性，可损害机体的组织和细胞，进而引起慢性疾病及衰老效应。紫外线照射及空气污染等外界因素也会使皮肤中产生过多的自由基，导致肌肤中的胶原蛋白受损，令肌肤失去弹性和光泽，肤色变得暗哑灰黄、出现皱纹，甚至破坏细胞组织等，出现老化现象。因此化妆品和保健品中常添加抗氧化剂清除皮肤和体内的自由基，达到护肤及延缓衰老的效果。
原花青素(OPC)是一种有着特殊分子结构的生物类黄酮，由儿茶素、表儿茶素及表儿茶素没食子酸酯通过C4-C6或C4-C8连接而成的不同聚合度的混合物。原花青素水溶性好,极易被机体吸收,生物利用度高达90％以上,它是目前自然界植物来源中发现的抗氧化、清除自由基能力最强的物质之一。原花青素是主要存在于黑枸杞、葡萄籽、银杏叶、柏树、松树皮、黑米种皮、玫瑰花等多种植物中的天然成分。申请公布号为CN 108619423 A的专利公开了一种原花青素抗衰老组合物及其制备工艺及其制备工艺和应用。
紫檀芪(Pterostilbene)是白藜芦醇的甲基化衍生物。现代药理研究表明，紫檀芪具有多种药理活性，比如抗氧化、抗炎等。由于紫檀芪存在两个甲氧基，因此紫檀芪具有亲脂性和吸收好、生物利用度高的优点。紫檀芪是主要存于蓝莓和紫檀心材中的天然成分。
但目前并没有将原花青素、紫檀芪组合作为美白抗皱剂应用于化妆品和保健品的报道。
发明内容
本发明的目的是提供一种能够高效美白、抗皱的组合物，能够有效美白、抗皱，同时安全不致敏。
本发明将原花青素B2、紫檀芪复配，结果发现二者复配后具有抗皱，美白协同作用，同时安全不致敏，减少了单一成分的使用量，从而实现了本发明的目的。
本发明的复配型天然美白抗皱组合物，其含有原花青素B2和紫檀芪作为活性成分。
优选，所述的原花青素B2和紫檀芪按物质的量比30-70:70-30组合，优选为1:1。
进一步优选，所述的原花青素B2和紫檀芪的浓度都为15～30μM/L。
本发明的第二个目的是提供上述复配型天然美白抗皱组合物在制备食品、保健品、化妆品中的应用，特别是美白淡斑、抗衰老的护肤品中的应用。
本发明的全部原料均来源于天然植物，2种提取物在一定的比例下进行配伍，协同增强美白及抗氧化效果。通过组分中各天然提取物间存在的相互促进作用，协调增强其美白功效，使得该组合物具有美白保湿、抗衰老、抗皱纹、淡化色斑等功效。同时能快速改善肤质、提高皮肤弹性、平缓皱纹等。
本发明方法得到的天然植物源美白抗皱组合物性质稳定，应用于护肤产品中无刺激、抗过敏、美白淡斑、抗衰老。本发明制备方法简单，功效显著，具有很好的应用前景。
附图说明：
图1是酪氨酸酶抑制率，图中的原花青素为原花青素B2；
图2是弹性蛋白酶抑制率，图中的原花青素为原花青素B2。
具体实施方式：
下面将通过实施例对本发明作进一步的描述，这些描述并不是对本发明内容作进一步的限定。本领域的技术人员应理解，对本发明内容所作的等同替换，或相应的改进，仍属于本发明的保护范围之内。
原花青素B2购自天津尖峰天然产物研究开发有限公司。紫檀芪为中国科学院华南植物园天然产物化学生物学实验室制备。
实施例1美白性能测定实验
酪氨酸酶是黑色素生成的关键酶，抑制酪氨酸酶的活性对延缓褐变和抑制黑色素形成具有重大意义，是美白皮肤的重要方法之一。在一定条件下，L-多巴能在酪氨酸酶的作用下生成多巴醌，该物质在475nm处有最大吸收峰，而底物L-多巴和酪氨酸酶吸收较小。若将待测样品加入其中，如果能够抑制酪氨酸酶的活性，势必会影响多巴醌的生成。多巴醌生成减少越多，说明待测物对酪氨酸酶的抑制作用越强，对酪氨酸酶抑制作用强就可作为美白的关键。
1、采用酶标仪来测定L-DOPA经催化生成的多巴醌的吸光度。按照表1所示，用移液枪向96孔酶标板中从左到右加入试剂(PBS和样品-原花青素B2、紫檀芪、阳性对照曲酸，其中样品的终浓度分别为如图1所示，用0.1％的DMSO溶液溶解)，加入50uL酪氨酸酶溶液(200U/mL，用0.1mmol/LpH 6.8磷酸缓冲溶液溶解)后将酶标板置于37℃人工气候箱孵育10min后，再加入50uL底物1mmol/L的L-DOPA溶液(用0.1mmol/LpH 6.8磷酸缓冲溶液溶解)，反应10min后，测定其在475nm处的吸光度。底物为L-DOPA。酪氨酸酶抑制率按下式计算。
式中：酪氨酸酶抑制实验参数见表1。
表1酪氨酸酶抑制实验参数
试验结果如图1所示，图1是原花青素B2和紫檀芪抑制酪氨酸酶活性的曲线，原花青素B2和紫檀芪对酪氨酸酶活性抑制率的IC50分别为59.2±3.3μM/L、48.7±3.1μM/L，所以两者抑制酪氨酸酶活性基本相当，原花青素B2的活性略强于紫檀芪。阳性对照曲酸对酪氨酸酶抑制率的IC50为21.2μM/L，这说明原花青素B2和紫檀芪为很好的天然酪氨酸酶抑制剂。
2、协同增效作用的试验
参照1的酪氨酸酶抑制实验，样品为原花青素B2、紫檀芪、原花青素B2和紫檀芪的混合物，具体如表2所示，
原花青素B2和紫檀芪单独或联合应用的酪氨酸酶抑制率如表2所示。通过CompuSyn软件分析，得到CI。由表2可见，原花青素B2和紫檀芪联合应用后的抑制率均比单独使用的抑制率高，且CI均<1，这说明原花青素B2和紫檀芪在酪氨酸酶抑制能力上联合应用具有协同增效的作用。
表2原花青素B2和紫檀芪对酪氨酸酶的协同抑制作用
实施例2抗皱效果检测和分析
皮肤作为人体最大的器官正是因为细胞外基质的丢失而产生衰老现象，据研究，皮肤厚度每十年大约减少6％。在皮肤的基质中，其中一种称为弹性蛋白酶的大分子纤维状蛋白，最为重要。皮肤出现松弛、皱纹、失去弹性等一系列老化现象大部分是由于弹性蛋白被降解或者变性，所以，可通过抑制弹性蛋白酶的活性来降低弹性蛋白的降解率，恢复皮肤的弹性、减少皱纹。
1、采用酶标仪用公知方法检测样品对弹性蛋白酶的抑制率。按照表3所示，在96孔板中加入样品溶液(原花青素B2或紫檀芪，终浓度如图2所示，溶剂为0.1％的DMSO溶液)和去离子水、130μL含有1.015mmol/L反应底物N-(甲氧基琥珀酰基)-L-丙氨酰-L-丙氨酰-L-脯氨酰-L-缬氨酸-4-硝基苯胺标准溶液的0.1mol/L Tris-Cl缓冲溶液(pH 8.0)，在25℃下温育5min，加入15μL弹性蛋白酶溶液(0.5U/mL)，继续在25℃下温育30min，然后测定410nm波长的吸光度(A410)。用去离子水替换样品水溶液，作为参比溶液同样测定吸光度(表3)，按下式计算抑制率。
式中：弹性蛋白酶抑制实验参数见表3。
表3弹性蛋白酶抑制实验参数
图2是原花青素B2和紫檀芪抑制弹性蛋白酶活性的曲线。原花青素B2和紫檀芪对弹性蛋白酶活性抑制率的IC50分别为75.7±3.8μM/L、73.2±3.1μM/L，所以两者都具有一定的抑制弹性蛋白酶的活性，是天然的弹性蛋白酶抑制剂。
2、协同增效作用
参照1的实验方法进行测试，只是样品如表4所示；
原花青素B2和紫檀芪单独或联合应用的弹性蛋白酶抑制率如表4所示。通过CompuSyn软件分析，得到CI。由表4可见，两种天然成分联合应用后的抑制率均比单独使用的抑制率高，且CI均<1，这说明原花青素B2和紫檀芪在弹性蛋白酶抑制能力上联合应用具有协同增效的作用。
表4原花青素和紫檀芪对弹性蛋白酶的协同作用
实施例3：天然美白抗皱组合物的制备和应用
在一容器中，取0.5g亚麻籽胶、0.2g洋槐胶溶于25g去离子水中，加热到60℃得到粘稠的液体一；取另一容器，加入0.5g原花青素B2、0.25g紫檀芪、混匀，再加入10g丁二醇、2g戊二醇与3g己二醇，溶解，添加去离子水58.55g，混匀得到液体二；将液体二加入到液体一中，搅拌25min后降温，得到乳液，即为具有美白、修复效果的天然乳液。
上述制备的天然乳液，用于具有美白及抗皱功效的护肤品中，含有该天然乳液的美白抗皱精华液如表5所示：
表5
将上述成分按其含量混合均匀，制得天然植物源美白抗皱组合物。
皮肤刺激性实验：以25名女性和25名男性为对象，50名志愿者早晚两次，涂抹0.05g天然植物源美白抗皱组合物于左前臂的正常皮肤上，连续测试七天，期间出现红肿、瘙痒、浮肿、丘疹均为产生过敏现象。
试验结果如表6所示：
表6
| 实验号 | 实施例3 |
| 出现过敏人数 | 0 |
通过本试验结果，可了解到实施例3的天然植物源美白抗皱组合物对皮肤无刺激，说明采用本发明的天然植物源美白抗皱组合物刺激性小，温和，安全性高。
为验证本发明效果，征集40名志愿者进行皮肤测试
测试对象：实验组为40名女性，年龄为25～45岁。所有志愿者每天使用所在组的实施例组合物，试验时间为一个月，在第30天时测试皮肤的弹性和美白程度。实验过程中不得使用其它任何护肤产品，而且受试者全部完成了验证过程，实验结果见表7。
表7
从上表结果可以看出，本发明的天然植物源美白抗皱组合物的试用均产生较好的效果，由此可见本发明的天然植物源美白抗皱组合物可以有效的提高皮肤弹性与皮肤光泽度，能使使用者皮肤明显变白。本实验组的志愿者均无过敏或其它毒副反应。
上述实施例为本发明较佳的实施方式，但本发明的实施方式并不受上述实施例的限制，其他的任何未背离本发明的精神实质与原理下所作的修改、等同替换、改进等均为等效的置换方式，都包含在本发明的保护范围之内。
Claims (2)
1.一种复配型天然美白抗皱组合物，其特征在于，由原花青素B2和紫檀芪组成并作为活性成分，所述的原花青素B2和紫檀芪按物质的量比1:1组合，所述的原花青素B2和紫檀芪的浓度都为15～30μmol/L。
2.权利要求1所述的复配型天然美白抗皱组合物在制备美白淡斑、抗衰老的护肤品中的应用。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010844858.1A CN111821209B (zh) | 2020-08-20 | 2020-08-20 | 一种复配型天然美白抗皱组合物和应用 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010844858.1A CN111821209B (zh) | 2020-08-20 | 2020-08-20 | 一种复配型天然美白抗皱组合物和应用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111821209A CN111821209A (zh) | 2020-10-27 |
| CN111821209B true CN111821209B (zh) | 2023-04-28 |
Family
ID=72918188
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010844858.1A Active CN111821209B (zh) | 2020-08-20 | 2020-08-20 | 一种复配型天然美白抗皱组合物和应用 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111821209B (zh) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113712834B (zh) * | 2021-10-19 | 2022-11-04 | 广州市胜梅化妆品有限公司 | 一种抗纹淡纹组合物及其制备方法和应用 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013016257A1 (en) * | 2011-07-25 | 2013-01-31 | Us Cosmeceu Techs Llc | Botanical antioxidant compositions and methods of preparation and use thereof |
| CN105377226A (zh) * | 2013-05-07 | 2016-03-02 | 欧莱雅 | 岩芹酸用以对抗身体体形的审美障碍的用途 |
| WO2016204715A1 (en) * | 2015-06-13 | 2016-12-22 | Jiangsu Fusheng Biotechlonogy Co. Ltd | Method of processing edible oil |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2528087B2 (ja) * | 1988-11-16 | 1996-08-28 | キッコーマン株式会社 | 美白化粧料 |
| TW201127417A (en) * | 2010-02-04 | 2011-08-16 | Ko-Ta Cheng | Tetrahydrocurcumin composite for whitening and anti-oxidation |
| CN103393579B (zh) * | 2013-08-08 | 2014-10-22 | 珀莱雅化妆品股份有限公司 | 一种美白修复脂质纳米粒乳液的制备方法 |
| CN104623670A (zh) * | 2013-11-06 | 2015-05-20 | 高松 | 一种含西红花素的组合物及其应用 |
| JP6173543B1 (ja) * | 2016-08-17 | 2017-08-02 | マイスターバイオ株式会社 | プテロスチルベン配糖体を含む外用組成物 |
| CN109939020A (zh) * | 2019-04-06 | 2019-06-28 | 天津大学 | 一种防晒美白保湿护肤剂及其制备方法 |
-
2020
- 2020-08-20 CN CN202010844858.1A patent/CN111821209B/zh active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013016257A1 (en) * | 2011-07-25 | 2013-01-31 | Us Cosmeceu Techs Llc | Botanical antioxidant compositions and methods of preparation and use thereof |
| CN105377226A (zh) * | 2013-05-07 | 2016-03-02 | 欧莱雅 | 岩芹酸用以对抗身体体形的审美障碍的用途 |
| WO2016204715A1 (en) * | 2015-06-13 | 2016-12-22 | Jiangsu Fusheng Biotechlonogy Co. Ltd | Method of processing edible oil |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111821209A (zh) | 2020-10-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11752168B2 (en) | Methods of using cosmetic compositions comprising exopolysaccharides derived from microbial mats | |
| KR20190082254A (ko) | 피부 향상 조성물 및 방법 | |
| Khunkitti et al. | In vitro bioactivities of clove buds oil (Eugenia caryophyllata) and its effect on dermal fibroblast | |
| CN111821209B (zh) | 一种复配型天然美白抗皱组合物和应用 | |
| CN105232395B (zh) | 一种美白爽肤水 | |
| CN114288216A (zh) | 一种抗氧化抗糖化组合物及其制备方法 | |
| CN107080252B (zh) | 具有美白保湿效果的组合物及其制备方法 | |
| Syakri et al. | Characterization and anti-aging tests of peel-off gel masks made from ethanolic extract of yarrow (Achillea millefolium) | |
| Mathen et al. | Evaluation of anti-elastase and antioxidant activity in antiaging formulations containing terminalia extracts. | |
| KR20130005616A (ko) | 피부미백용조성물 | |
| CN112353722A (zh) | 一种保湿抗衰修复组合物及其制备方法和应用 | |
| DE69905603T2 (de) | Walnusssamenschrotmehlextrakt | |
| KR101398392B1 (ko) | 가막살나무 추출물을 포함하는 피부주름 개선용 화장료조성물 | |
| KR20100061978A (ko) | 피부미백용조성물 | |
| TWI757860B (zh) | 抗氧化美白組合物及其製備方法和應用 | |
| Mat Yasin et al. | Antioxidant and Enzyme Inhibitory Activities of Areca catechu, Boesenbergia rotunda, Piper betle and Orthosiphon aristatus for Potential Skin Anti-aging Properties. | |
| Laxane et al. | Antioxidant studies on the ethanolic extract of Zornia gibbosa | |
| Gupta et al. | Anti-ageing activity and antioxidant activity of methanol extracts of leaves and flowers of Salvia officinalis and Rosmarinus officinalis | |
| CN114796046B (zh) | 一种美白淡斑抗氧化组合物及其应用 | |
| CN110897957B (zh) | 一种植物提取物组合物和制备方法及其在化妆品中的应用 | |
| KR101272793B1 (ko) | 대나무 잎 에탄올 추출물 및 대나무 오일을 포함하는 화장료 조성물 | |
| CN110585088B (zh) | 一种皮肤焕亮组合物及其制备方法和应用 | |
| CN117357445A (zh) | 一种美白抗氧化组合物及其制备方法和应用 | |
| CN118576529A (zh) | 敏感肌可用的美白抗皱组合物及制备方法和应用 | |
| CN117815135A (zh) | 含有北美乔松树皮提取物的美白淡斑组合物 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |