201127417 六、發明說明： 【發明所屬之技術領域】 本發明是有關於一種組合物 於美白與抗氧化之月桃素組合物。特別疋有關於一種用 【先前技術】 化妝大眾對外表皮膚的保養越來越重視，於 =養4%主要為女性消費較多。每當 年慶時’各家保養品專櫃皆出 J週 將百貨公司擠得水::現欲購貝之人潮’消費者 非吊夕的男性消費者加入愛美 负 妝保養品主攻男且有越來越多的化 逐漸:低，注重美白保養之年齡層也 斬降低因此，各家保養品業者 新，並積極研菸如行姑々ώ A 匕妝保養口口推陳出 氧化等功效===妝保養品提升美白或抗 膚底層。^冑化妝保養品更能吸收並滲透至皮 以上以功效，而兒茶素具有抗氧化功效， =之!:ί?:惟:其彼此無法兼具美白及 而兒茶 ==效，亦嶋素無具;抗氧二，而兒茶 。、…、、有美白功能。因此，於應用上 具美白或抗氧化之效能時，即需同時包人：欲k供兼 如此一來，其成本則較為高昂時包含此_質。 【發明内容】 201127417 有鑑於上述習知技藝之問題，本發明之目的就是在 提供一種用於美白與抗氧化之月桃素組合物，以達到成 本低廉與美白及抗氧化之功效。 根據本發明之目的，提出一種用於美白與抗氧化之 月桃素組合物，其包含月桃素（tetrahydrocurcumin)、白 黎蘆醇（resveratrol)、紫檀芪（pterostilbene)及核醣核酸修 補複合體。其中月桃素、白藜蘆醇、紫檀芪及核醣核酸 修補複合體，於月桃素組合物中各佔0.1〜5重量百分比。 在本發明之所述之月桃素組合物中，更可包括細胞 長壽因子、龍血樹萃取物及美白因子，於月桃素組合物 中分別佔2.5〜5重量百分比、2.5〜5重量百分比及 0.25〜0.5重量百分比。 此外，本發明之所述之月桃素組合物中亦可包括牛 奶蛋白（milk protein)、玻尿酉变（sodium hyaluronate)、維生 素E、尿囊素（allantoin)、維生素B12或維生素C醣苷 (Ascorbic-Acid 2-Glucoside)。其中，牛奶蛋白、玻尿酸、 維生素E、尿囊素、維生素B12及維生素C醣苷可分別 佔本發明之月桃素組合物之2.5〜5重量百分比、1.5〜3重 量百分比、0.25〜0.5重量百分比、0.025〜0.05重量百分 比、0.5〜1重量百分比及0.25〜0.5重量百分比。 承上所述，依本發明之用於美白與抗氧化之月桃素 組合物，其可具有一或多個下述優點： (1)本發明之月桃素組合物係取自天然成分，易於 皮膚吸收且無副作用。 201127417 (2)本發明之月桃素組合物可有效抑制黑色素形 成，並且有效去除自由基，以達到美白及抗氧化之功效。 【實施方式】 實施例1:本發明之月桃素組合物之組成 本發明之用於美白與抗氧化之月桃素組合物，其包 含月桃素（tetrahydrocurcumin)、白藜蘆醇（resveratrol)、 紫檀芪（pterostilbene)及核核酸修補複合體。其中’月 桃素、白藜蘆醇、紫檀芪及核醣核酸修補複合體’於月 桃素組合物中各佔0.1〜5重量百分比。其中，去氧核膽 核酸修補複合體可為比菲德氏菌發酵萃取液（Bifida Ferment Lysate)。 在本發明之所述之月桃素組合物中，更可包括細胞 長壽因子、龍血樹萃取物及美白因子，於該月桃素組合 物中分別佔2.5〜5重量百分比、2.5〜5重量百分比及 0.25〜0.5重量百分比。其中，細胞長壽因子可包括丁烯 乙二醇及酵母菌/盾葉鬼臼發酵濾液萃取物（butylene Glycol and saccharomyces/Podophyllum Peltatum filtrate extrct) »而龍血樹萃取物可包括前花青素 (proanthocyanidins)、塔斯品驗（taspine)或木質素 (lignans)。美白因子則可為虎耳草萃取物（Saxifraga Sarmentosa Extract)、葡萄萃取物（Vitis Vinifera Fruit Extract)、桑椹根部萃取物（Morus Bombycis Root Extract) 或黃枣根部萃取物（Scutellaria Baicalensis Root Extract)。 201127417 在本發明之用於美白與抗氧化之月桃素組合物中， 更可包括牛奶蛋白（milk pr〇tein)、玻尿酸（s〇di謂 hyaluronate)、維生素E、尿囊素（allant〇in)、維生素B工2 或維生素C餹苦（Ascorbic-Acid 2-Glucoside)，進而增強 本發明之月桃素組合物之美白與抗氧化之效果。其中， 牛奶蛋白、玻尿酸、維生素E、尿囊素、維生素B12及 維，素C料可分別佔本發明之月桃素組合物之2.5〜$ 重1百分比、1.5〜3重量百分比、〇 25〜〇 5重量百分比、 0.0:5 0.05 ί量百分比、o w重量百分比及〇 〜〇 5 重量百刀比。其中，尿囊素可具有抗炎、舒緩及幫助皮 膚，織再生功能。玻尿酸為強效保濕劑，可吸收其本身 重置1000倍水分，為最佳的保濕劑之一。而維生素Ε 可作為皮膚内的抗氧化劑，以清除自由基，進而保護膠 原蛋白和脂質等免受自由基的攻擊，並可減少紫外線對 人體的傷害。此外’維生素£可從皮膚内層產生保满作 用，進而滋潤皮膚及減少粗糙。除此之外，維生素£ 可促進傷口癒合。 較佳地’本發明之月桃素組合物可為粉末狀、顆粒 狀或液體狀。當為粉末狀時，可加人清水混合均句，並 塗，於欲美白之皮膚上。此外，本發明之月桃素組合物 可更包括-醫樂可接受之賦形劑或载體，進而將本發明 之月桃素組合物製成粉餅、蜜粉、粉底霜、面膜或化妝 2保養品。其中，賦形劑可選自由防腐劑、抗氧化劑、 螯合劑、等滲透劑、潤滑劑、錠劑佐劑、著色劑、保濕 劑、結合劑、皮膜成形劑、緩衝溶劑、乳化劑、酸驗中 201127417 和劑及醫藥可相容載劑所組成之群組中。又，酸鹼中和 劑可為二乙醇胺，進而調節月桃素組合物中的酸驗度。 皮膜成形劑可為壓克力尚分子膠（acrylate/C 1 〇_3〇 alkyl acrylates crosspolymer)。 實施例2 :本發明之月桃素組合物之美白測試 黑色素細胞（melanocytes)具有合成黑色素（melanin) 的作用。黑色素細胞是影響皮膚顏色的重要細胞，亦是 形成地球上不同膚色人種的主要原因。當黑色素細胞受 到紫外線刺激時，會活化酪胺酸酵素（tyr〇sinase)，催化 酪胺酸（tyrosine)加速氧化形成多巴（d〇pa)色素，再經過 連串氧化作用而形成黑色素（melanin)。通常黑色素會 隨著角質細胞角化的過淨呈’逐漸上#至皮膚表面，使得 黑色素隨著角質層的剝落而代謝。 因此201127417 VI. Description of the Invention: TECHNICAL FIELD OF THE INVENTION The present invention relates to a composition for whitening and anti-oxidation of a moon peach composition. In particular, there is a use of [Prior Art] Make-up people pay more and more attention to the maintenance of external skin, and 4% of them are mainly consumed by women. Whenever the annual celebration, 'all the skin care products counters are out of the J week, the department store is squeezed into the water:: Now want to buy the people of the shell. The consumer is not the hang of the male consumer to join the beauty of the negative makeup and maintenance products, the main attack on men and more and more The more the gradualization is: low, the age layer that pays attention to the whitening maintenance is also reduced. Therefore, the various skin care products are new, and actively researching smoke, such as aunts, 匕 makeup maintenance, oral oxidative effects, etc. === Make-up and skin care products enhance whitening or anti-mapping. ^胄 Make-up and maintenance products can absorb and penetrate into the skin to improve the effect, while catechins have antioxidant effect, =!!: ί?: Only: they can not both have whitening and catechu == effect, also 嶋No food; no oxygen, but catechu. ,...,, have whitening function. Therefore, when applying whitening or anti-oxidation effects, it is necessary to include both people at the same time: if the cost is higher, the cost is higher. SUMMARY OF THE INVENTION 201127417 In view of the above-mentioned problems of the prior art, it is an object of the present invention to provide a moon white composition for whitening and anti-oxidation to achieve cost-effective and whitening and anti-oxidation effects. In accordance with the purpose of the present invention, a composition for whitening and anti-oxidation of a moon peach comprising a tetrahydrocurcumin, a resveratrol, a pterostilbene and a ribonucleic acid repair complex is provided. The moon peach, resveratrol, rosewood and ribonucleic acid repair complex each comprise 0.1 to 5 weight percent in the moon peach composition. In the moon peach composition of the present invention, the cell longevity factor, the dragon blood tree extract and the whitening factor may further comprise 2.5 to 5 weight percent and 2.5 to 5 weight percent in the moon peach composition, respectively. And 0.25 to 0.5 weight percent. In addition, the moon peach composition of the present invention may also include milk protein, sodium hyaluronate, vitamin E, allantoin, vitamin B12 or vitamin C glycoside ( Ascorbic-Acid 2-Glucoside). Wherein, milk protein, hyaluronic acid, vitamin E, allantoin, vitamin B12 and vitamin C glycoside may respectively account for 2.5 to 5 weight percent, 1.5 to 3 weight percent, 0.25 to 0.5 weight percent of the moon peach composition of the present invention, 0.025 to 0.05% by weight, 0.5 to 1% by weight, and 0.25 to 0.5% by weight. According to the present invention, the moon white and anti-oxidation moon peach composition according to the present invention may have one or more of the following advantages: (1) The moon peach composition of the present invention is derived from a natural component, Easy to absorb skin without side effects. 201127417 (2) The moon peach composition of the present invention can effectively inhibit the formation of melanin and effectively remove free radicals to achieve whitening and anti-oxidation effects. [Embodiment] Example 1: Composition of the Mooncake Composition of the Present Invention The Moonberry and Antioxidant Mooncake Composition of the present invention comprises tetrahydrocurcumin and resveratrol. , pterostilbene and nuclear nucleic acid repair complex. Wherein 'moon peach, resveratrol, rosewood and ribonucleic acid repair complex' each account for 0.1 to 5 weight percent of the composition of the moon. The deoxyribonucleoside repair complex may be a Bifida Ferment Lysate. In the moon peach composition of the present invention, the cell longevity factor, the dragon blood tree extract and the whitening factor may further comprise 2.5 to 5 weight percent and 2.5 to 5 weight, respectively, in the moon peach composition. Percentage and 0.25 to 0.5 weight percent. Among them, the cell longevity factor may include butylene glycol and yeast / glucoomyces / Podophyllum Peltatum filtrate extrct » and the dragon blood tree extract may include proanthocyanidins ), taspine or lignans. The whitening factor may be Saxifraga Sarmentosa Extract, Vitis Vinifera Fruit Extract, Morus Bombycis Root Extract or Scutellaria Baicalensis Root Extract. 201127417 In the moon white and anti-oxidation moon peach composition of the present invention, milk protein (milk pr〇tein), hyaluronic acid (s〇di called hyaluronate), vitamin E, allantoin (allant〇in) may be further included. ), vitamin B 2 or vitamin C (Ascorbic-Acid 2-Glucoside), thereby enhancing the whitening and anti-oxidation effects of the moon peach composition of the present invention. Among them, milk protein, hyaluronic acid, vitamin E, allantoin, vitamin B12 and vitamin C can account for 2.5 to $1%, 1.5 to 3 weight percent, 〇25~ of the moon peach composition of the present invention, respectively. 〇 5 weight percent, 0.0:5 0.05 liter percent, ow weight percent, and 〇~〇5 weight percent ratio. Among them, allantoin can have anti-inflammatory, soothing and skin-reinforcing functions. Hyaluronic acid is a powerful moisturizer that absorbs itself and resets it by 1000 times as one of the best moisturizers. Vitamin Ε can be used as an antioxidant in the skin to scavenge free radicals, thereby protecting collagen proteins and lipids from free radicals and reducing the damage of UV rays to the human body. In addition, vitamins can be used to protect the skin from the inner layers of the skin, thereby moisturizing the skin and reducing roughness. In addition, vitamins £ promote wound healing. Preferably, the moon peach composition of the present invention may be in the form of a powder, a granule or a liquid. When it is in the form of a powder, it can be mixed with water and applied to the skin that is intended to whiten. In addition, the moon peach composition of the present invention may further comprise an excipient or carrier acceptable to the medicinal, and further, the moon peach composition of the present invention is made into a powder cake, a powder, a foundation cream, a mask or a makeup 2 Care products. Among them, excipients can be selected from preservatives, antioxidants, chelating agents, penetrants, lubricants, lozenge adjuvants, colorants, humectants, binders, film forming agents, buffer solvents, emulsifiers, acid tests. Medium 201127417 is a group of agents and pharmaceutical compatible carriers. Further, the acid-base neutralizer may be diethanolamine, thereby adjusting the acidity of the composition in the moon peach composition. The film forming agent may be an acrylate/C 1 〇 〇 3 〇 alkyl acrylates crosspolymer. Example 2: Whitening test of the moon peach composition of the present invention Melanocytes have the effect of synthesizing melanin. Melanocytes are important cells that affect the color of the skin and are the main reason for the formation of different races on the planet. When melanocytes are stimulated by ultraviolet light, it activates tyr〇sinase, catalyzes the accelerated oxidation of tyrosine to form dopa pigment, and then undergoes a series of oxidation to form melanin (melanin). ). Usually, melanin will go through the keratinocyte keratinization and gradually increase to the skin surface, so that melanin is metabolized with the exfoliation of the stratum corneum. therefore
方例之美白測试係利用體外（in vitr〇)抑 制赂胺I酵素的效果，探討本發明之月桃素組合物對黑 =素开>成抑制▲力。此實驗設計係將受測檢品與酿胺酸 =培養（實驗組），其中受測檢品係為本發明之月桃素 :且&物利用緩衝溶液配製成溶液(G.5 mg/ml)。取本發明 醢”綠 色管)中，再依序加入酪氨酸 25^γ、Λ㈣溶液、赃S緩衝液及_。接著，於 、皮長4 7 / Ϊ 3 G分鐘’藉由紫外光/可見域譜儀測定 度’叫發以月桃素組 列公式^算成之抑制率。黑色素生成抑制率則以下 201127417 )xlOO°/〇 抑制率(％)=(1- 實驗組O.D.值 控制組O.D.值 其中，實驗組O.D.值係為加有受測檢品之多吸收 度，控制組Ο.D.值係為未加受測檢品之吸收度。如表一 所示，其實驗結果顯示本發明之月桃素組合物抑制黑色 素生成之抑制率可達32.5%，因此，本發明之月桃素組 合物係具有明顯抑制黑色素生成的功效，係具有美白效 果。 表一 樣品 O.D·值 抑制率（％) 控制組 1.063 0.0 實驗組 0.717 32.5 當本發明之月桃素組合物濃度越高時，其抑制酪氨 酸酶之抑制率越高，如第1圖所示。此外，本發明之月 桃素組合物抑制酷·氨酸酶之半抑制激度（the half-inhibition concentration，IC5〇)約為 0.88 mg/ml。而熊 果素及維生素C2之半抑制濃度分別約為1.8 mg/ml及3 mg/ml，故本發明之月桃素組合抑制酪氨酸酶之功效較 高。 實施例3:本發明之月桃素組合物之自由基清除測試 於抗氧化之研究上，常使用 DPPH (l，l-diphenyl-2_picryhydrazyl)評估抗氧化物之供氫能 201127417 力。合有DPPH之甲醇溶液中，於517nm下有較強的吸 光值。被受測檢品還原時，其吸光值會較低，而吸光值 越低，表示受測檢品之抗氧化能力越強。 將本發明之月桃素組合物配置不同濃度之待測溶 液，分別有 0、1.25、2 5、3 75、5 ；5 6 址从 '° 乂/3、5 及 6.25 ppm。接著，The whitening test of the prescription uses the in vitro (in vitr〇) inhibitory effect of the amine I enzyme to investigate the effect of the moonmelon composition of the present invention on black = prime. This experimental design is to test the test article with tyrosine = culture (experimental group), wherein the test article is the moon peach of the present invention: and & the solution is prepared into a solution using a buffer solution (G.5 mg /ml). Take the 醢"green tube" of the present invention, and then add tyrosine 25^γ, Λ(4) solution, 赃S buffer and _. Then, the skin length is 4 7 / Ϊ 3 G minutes 'by ultraviolet light / The degree of measurement of the visible field spectrometer is called the suppression rate of the formula of the moon peach group. The inhibition rate of melanin production is below 201127417. xlOO°/〇 inhibition rate (%)=(1 - experimental group OD value control group Among the OD values, the OD value of the experimental group is the multi-absorbance of the test article, and the control group Ο.D. value is the absorbance of the untested product. As shown in Table 1, the experimental results show The inhibition rate of the melanin composition of the present invention inhibits the formation of melanin by up to 32.5%. Therefore, the moon peach composition of the present invention has the effect of significantly inhibiting melanin production, and has a whitening effect. Table 1 Sample OD value inhibition Rate (%) Control group 1.063 0.0 Experimental group 0.717 32.5 When the concentration of the moon peach composition of the present invention is higher, the inhibition rate of inhibition of tyrosinase is higher, as shown in Fig. 1. Further, the present invention The moon peach composition inhibits the half-inhibition of the cold-lysinase (the half-inhibi The concentration of IC5〇 is about 0.88 mg/ml. The half inhibitory concentration of arbutin and vitamin C2 is about 1.8 mg/ml and 3 mg/ml, respectively. Therefore, the combination of the moon peach combination of the present invention inhibits tyrosinase. Example 3: Free radical scavenging test of the moon peach composition of the present invention In the study of antioxidants, DPPH (l, l-diphenyl-2_picryhydrazyl) is often used to evaluate the hydrogen supply energy of antioxidants 201127417. In the methanol solution with DPPH, it has a strong absorbance at 517 nm. When it is reduced by the test, its absorbance will be lower, and the lower the absorbance, the stronger the antioxidant capacity of the test. The moon peach composition of the present invention is configured with different concentrations of the solution to be tested, which are 0, 1.25, 2 5, 3 75, 5; 5 6 addresses from '° 乂 / 3, 5 and 6.25 ppm. Then,
分別取不同濃度之待測溶液〇 4 ml加入1〇〇 μΜ DppH 之甲醇溶液中’且室溫中靜置1G分鐘。1G分鐘後，藉 由紫外光/可見光光譜儀測定波長517 nm時之吸收度: 且實驗執打二重覆，以增加本發明之月桃素組合物之抗 氧化能力的精準度。而抑制率之計算公式如下：其結果 如表二及第2圖所示。 抑制率(％) = (1測濃度之O.D.值、 待測濃度為〇 ppm之O.D.值X1 〇〇% 表二 待測溶 液之濃 度(ppm) 第一次 第二次 第三次 平均 抑制 率（％) —0 0.434 0.415 0.416 0.422 — -«« 〇 _ 1.25 0.313 0.317 0.32 0.317 \J 24 2.5 0.237 0.242 0.238 0.239 3.75 0.185 0.183 0.187 0.185 一 1 ~ —5 0.154 0.143 0.146 0.148 65 64 6.25 0.125 0.121 0.125 0.124 70 201127417 結果顯示’本發明之月桃素組合物濃度越高時，其 自由基清除率則越高。且本發明之月桃素組合物之清除 DPPH自由基之IC50濃度約為3.15 ppm (9 μΜ)。 因此’利用本發明之月桃素組合物可有效抑制黑色 素形成’並且有效去除自由基，以達到美白及抗氧化之 功效。 以上所述僅為舉例性，而非為限制性者。任何未脫 離本發明之精神與範疇，而對其進行之等效修改或變 更’均應包含於後附之申請專利範圍中。 【圖式簡單說明】 第1圖係為本發明之用於美白與抗氧化之月桃素組合 物抑制酪氨酸酶之抑制率之曲線圖；以及 第2 ®係為本發明之用於美白與抗氧化之月桃素組合 物清除DPPH自由基之抑制率之曲線圖。 【主要元件符號說明】 益Take 4 ml of different concentrations of the test solution, add 1 μm of DppH in methanol solution, and let stand for 1 G minutes at room temperature. After 1 G minutes, the absorbance at a wavelength of 517 nm was measured by an ultraviolet/visible spectrometer: and the experiment was double-coated to increase the accuracy of the antioxidant capacity of the mooncake composition of the present invention. The formula for calculating the inhibition rate is as follows: The results are shown in Table 2 and Figure 2. Inhibition rate (%) = (1 OD value of the measured concentration, the OD value of the test concentration is 〇ppm X1 〇〇% Table 2 The concentration of the solution to be tested (ppm) The second time the third time average inhibition rate (%) —0 0.434 0.415 0.416 0.422 — —«« 〇_ 1.25 0.313 0.317 0.32 0.317 \J 24 2.5 0.237 0.242 0.238 0.239 3.75 0.185 0.183 0.187 0.185 A 1 ~ —5 0.154 0.143 0.146 0.148 65 64 6.25 0.125 0.121 0.125 0.124 70 201127417 The higher the concentration of the moon peach composition of the present invention, the higher the radical scavenging rate. The IC50 concentration of the DPPH free radical of the present invention is about 3.15 ppm (9 μΜ). 'Utilizing the moon peach composition of the present invention can effectively inhibit melanin formation' and effectively remove free radicals to achieve whitening and anti-oxidation effects. The above is merely illustrative, not limiting. The spirit and scope of the invention, and the equivalent modifications or alterations thereof are included in the scope of the appended patent application. [Simplified illustration of the drawings] Figure 1 is the beauty of the invention. A graph of inhibition of tyrosinase inhibition by an antioxidant antioxidant composition; and a second ® is the inhibition rate of the DPPH free radicals of the present invention for whitening and anti-oxidation of the moon pigment composition Curve. [Main component symbol description]