CN111771563A - Method for researching influence of plant hormone on plants - Google Patents
Method for researching influence of plant hormone on plants Download PDFInfo
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- CN111771563A CN111771563A CN202010505541.5A CN202010505541A CN111771563A CN 111771563 A CN111771563 A CN 111771563A CN 202010505541 A CN202010505541 A CN 202010505541A CN 111771563 A CN111771563 A CN 111771563A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
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- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention provides a method for researching the influence of plant hormones on plants, which comprises the following steps: step 1, creating a wound part on a test plant; step 2, covering a fibrous porous material on the surface of the wound part; step 3, coating a shading base material on the outer surface of the fibrous porous material; and 4, injecting the plant hormone into the fibrous porous material. The fibrous porous material adsorbed with the plant hormone to be detected is applied to the surface of the wound part of the plant to be detected, so that the volatilization speed of the plant hormone to be detected can be effectively reduced, the plant hormone can be gradually released to the wound part in the experimental process, the drug effect of the plant hormone is prolonged, the utilization rate of the plant hormone is improved, the volatilization amount of the plant hormone in the air is reduced, and the environment friendliness is improved. On the other hand, the shading base material is used for blocking the plant hormone adsorbed on the fibrous porous material from the external sunlight, and the photosensitive plant hormone is prevented from being degraded under the condition of illumination.
Description
Technical Field
The invention belongs to the field of plant experiments, and particularly relates to a method for researching the influence of plant hormones on plants.
Background
Plant hormones are secondary metabolites in plants and play an important regulatory role in the whole life process of plants. Phytohormones regulate the growth and differentiation of cells, the development of organs, the dormancy and germination of seeds, the senescence and abscission of leaves, and the like. The regulation of plant physiological activities by plant hormones can involve changes in their concentrations. The change of the composition and the content of the hormone in the plant body is mastered, so that the interaction among the plant hormones is found.
In research studies on plant hormone regulation of plant growth, plant hormones are generally applied directly to test plants, and the effects of the plant hormones on plant growth are characterized by observing and analyzing the growth state of each plant after a certain period of cultivation. At present, the following methods are commonly used for applying phytohormones:
(1) solution spraying: spraying plant hormone solution to the leaves, fruits or the whole plant, wherein the dosage of the plant hormone solution is determined by the wetting degree of the sprayed part. When the solution is sprayed, the solution is distributed in a large area in a planting area, the defects of raw material waste and environmental pollution are caused, in addition, growth hormone attached to the surface of a plant can be easily volatilized, and the retention time on the plant is too short to be absorbed by the plant.
(2) Dropping the solution: the plant hormone is dripped into the growing point of the stem and is mainly used for treating the growing point at the top of the plant stem, flowers or dormant buds and the like. The method can be used for quantitatively applying the medicament, and the dosage is accurate, but the method is difficult to apply in fields.
(3) Soaking in a solution: soaking the base of plant seed, root tuber, stem or leaf in solution containing plant hormone for a certain time, taking out, and sowing or cutting. The method requires a large amount of phytohormone.
(4) Solution smearing: the prepared plant hormone is directly smeared on the parts needing to be treated, such as the cut of leaves, buds, stems and branches, and the like by using a brush pen or other tools so as to observe certain physiological effects of a certain hormone on plants. The time for maintaining the drug effect of the solution after being smeared is short.
(5) Solution soil irrigation: the prepared plant hormone water aqua is poured into soil or mixed with fertilizer and soil according to a certain concentration for application, and plants are absorbed into the body through root systems. The method has certain application limitation, for example, the common plant hormone-growth inhibiting substance B9 is not easy to move in soil, is easy to be decomposed by soil microorganisms, and is not suitable for solution soil irrigation.
(6) Solution culture: adding certain concentration of plant hormone into the plant culture solution, transferring the germinated seeds or seedlings into the culture solution, and allowing the roots to absorb nutrients and hormone at the same time. However, during the cultivation, there is also a problem that the phytohormones are easily volatilized and thus cannot be sufficiently absorbed by the plants.
(7) Solution injection: drilling a hole in the fruit tree trunk, wherein the depth of the hole is 1/3-1/2 of the diameter of the tree trunk; then the prepared plant hormone aqueous solution is put into a container, one end of the container is connected with a thin rubber tube, a small section of thin glass tube is inserted into the tube opening, a small block of foam material is plugged into the tube opening to adjust the dropping speed, so that the plant hormone aqueous solution slowly drops out in a dropping shape, and then the glass tube is inserted into the drilled hole and is absorbed by the fruit tree trunk.
Disclosure of Invention
The invention aims to provide a method for researching the influence of plant hormone on plants so as to optimize the application effect of the plant hormone,
According to one aspect of the present invention, there is provided a method of studying the effect of plant hormones on plants comprising the steps of: step 1, creating a wound part on a test plant; step 2, covering a fibrous porous material on the surface of the wound part; step 3, coating a shading base material on the outer surface of the fibrous porous material; and 4, injecting the plant hormone into the fibrous porous material.
The fibrous porous material adsorbed with the plant hormone to be detected is applied to the surface of the wound part of the plant to be detected, so that the volatilization speed of the plant hormone to be detected can be effectively reduced, the plant hormone can be gradually released to the wound part in the experimental process, the drug effect of the plant hormone is prolonged, the utilization rate of the plant hormone is improved, the volatilization amount of the plant hormone in the air is reduced, and the environment friendliness is improved. On the other hand, the shading base material is used for blocking the plant hormone adsorbed on the fibrous porous material from the external sunlight, and the photosensitive plant hormone is prevented from being degraded under the condition of illumination.
Preferably, step 4 is followed by step 5: and 5, periodically supplementing phytohormone into the fibrous porous material. Optionally, in steps 4 and 5, plant hormones are added to the fibrous porous material until the fibrous porous material is just fully wetted. In the experimental process, the fibrous porous material and the shading base material are not required to be detached when the plant hormone is added into the porous material every time, liquid adding tools such as a liquid-transferring gun can be directly adopted to directly add the plant hormone into the fibrous porous material, so that the operation of supplementing the plant hormone is simple and rapid, and more importantly, the damage to the plant to be detected caused by replacing the fibrous porous material and the shading base material can be avoided.
Preferably, the opacifying substrate is a barrier material. For the phytohormone adsorbed on the fiber type porous material, the shading base material not only can play a role in shading light, but also can play a role in moisturizing, the volatilization of the phytohormone can be effectively resisted, the effective utilization rate of the phytohormone is greatly improved, and in addition, the condition that the shading base material is damaged due to the fact that the phytohormone permeates the shading base material is also avoided.
Preferably, the light-screening substrate is a plastic material. The shading material can keep a specific shape through simple operation without other consumables, and is favorable for being fixed on a plant to be detected.
Preferably, the light-shielding substrate is a tinfoil paper. The tin foil paper is cheap and easy to obtain, is non-toxic and safe, and has the characteristics of shading, seepage prevention and plasticity.
Preferably, in step 3: and wrapping the plant to be tested and the carbon fiber-shaped porous material by using the tin foil paper, and kneading the tin foil paper until the tin foil paper and the wrapped carbon fiber-shaped porous material are fixed on the plant to be tested.
Preferably, in step 3, the fibrous porous material is hermetically wrapped with tinfoil.
The tin foil paper can limit the fibrous porous material adsorbed with the plant hormone, and the fibrous porous material is ensured to be coated on the surface of the wound part. The tin foil paper is pressed to be enough to seal the reserved empty position relative to the reserved empty position on the surface of the plant to be tested, so that errors caused by illumination and liquid medicine volatilization on experimental results are avoided. In addition, due to the plasticity of the tin foil, when the plant hormone needs to be supplemented, the tin foil is directly lifted at a proper position, a sample inlet with a certain size is reserved, and after sample introduction is finished, the tin foil is pinched to restore the sealing and wrapping state of the tin foil to the fibrous porous material.
Preferably, the fibrous porous material is absorbent cotton.
The tin foil paper and the absorbent cotton adopted by the invention are simple and easily available, low in price and free from toxicity, the influence of the phytohormone on the plant can be researched by utilizing the consumables through simple operation, the influence of factors such as illumination, volatilization and the like is avoided, and the reliability and the accuracy of an experimental result are effectively improved.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The phytohormone to be tested in this example was auxin, and the reference plant was a pea plant. Auxin was applied to pea plants as follows:
pretreatment: selecting clean absorbent cotton balls, unfolding the absorbent cotton into strips, cutting the strips into small pieces, cutting the clean tinfoil into specifications of 4cm in width and 6cm in length, and folding the strips into a rectangular shape;
step 1, carrying out topping treatment on pea plants, thereby creating a wound part;
step 2, paving absorbent cotton on the surface of the wound part of the pea plant;
step 3, wrapping the degreasing cotton with the tin foil paper, winding the tin foil paper on the pea plants for two circles, enabling the tin foil paper to be tightly attached to the outer side faces of the pea plants and the degreasing cotton, then tightly pinching vacant positions reserved between the upper end and the lower end of the tin foil paper and the pea plants until the tin foil paper and the degreasing cotton are fixed on the pea plants, and hermetically wrapping the degreasing cotton with the tin foil paper;
and 4, step 4: lifting the upper end of the tin foil paper, leaking the gap to form a sample inlet, and injecting auxin into the absorbent cotton by using a liquid transfer gun until the whole absorbent cotton is just wetted;
and 5, supplementing the auxin into the absorbent cotton every 12 hours, so that the absorbent cotton is kept wet.
Example 2
The phytohormone to be tested in this example was cytokinin, and the test plant was a tomato seedling. Cytokinins were applied to tomato seedlings as follows:
pretreatment: selecting clean absorbent cotton balls, unfolding the absorbent cotton into strips, cutting the strips into small pieces, cutting the clean tinfoil into specifications of 4cm in width and 6cm in length, and folding the strips into a rectangular shape;
step 1, carrying out topping treatment on tomato seedlings so as to create a wound part;
step 2, paving absorbent cotton on the surface of the wound part of the tomato seedling;
step 3, wrapping the absorbent cotton with the tinfoil paper, winding the tinfoil paper on the tomato seedlings for two circles, enabling the tinfoil paper to be attached to the outer side surfaces of the tomato seedlings and the absorbent cotton, then pinching the vacant sites reserved between the upper end and the lower end of the tinfoil paper and the tomato seedlings until the tinfoil paper and the absorbent cotton are fixed on the tomato seedlings, and hermetically wrapping the absorbent cotton with the tinfoil paper;
and 4, step 4: lifting the upper end of the tin foil paper, leaking the gap to form a sample inlet, and injecting cytokinin into the absorbent cotton by using a liquid transfer gun until the whole absorbent cotton is just wetted;
and 5, supplementing cytokinin into the absorbent cotton every 8 hours, so that the absorbent cotton is kept wet.
Although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the true spirit and scope of the present invention.
Claims (8)
1. A method of studying the effect of plant hormones on plants comprising the steps of:
step 1, creating a wound part on a test plant;
step 2, covering a fibrous porous material on the surface of the wound part;
step 3, coating a shading base material on the outer surface of the fibrous porous material;
and 4, injecting plant hormone into the fibrous porous material.
2. The method for studying the effect of plant hormones on plants as claimed in claim 1, further comprising step 5 after said step 4:
and 5, periodically supplementing the plant hormone into the fibrous porous material.
3. A method of investigating the effect of plant hormones on plants as claimed in any one of claims 1 or 2 wherein: the shading base material is made of impermeable material.
4. A method of investigating the effect of plant hormones on plants as claimed in claim 3 wherein: the shading base material is a plastic material.
5. The method of studying the effect of plant hormones on plants of claim 4 wherein: the shading base material is tin foil paper.
6. The method of studying the effect of plant hormones on plants as claimed in claim 5, wherein in said step 3:
and wrapping the plant to be tested and the carbon fiber-shaped porous material by using the tin foil paper, and kneading the tin foil paper until the tin foil paper and the wrapped carbon fiber-shaped porous material are fixed on the plant to be tested.
7. The method of studying the effect of plant hormones on plants of claim 6 wherein: and in the step 3, the fibrous porous material is hermetically wrapped by the tin foil paper.
8. A method of investigating the effect of plant hormones on plants as claimed in any one of claims 1 or 2, wherein: the fibrous porous material is absorbent cotton.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202010505541.5A CN111771563A (en) | 2020-06-05 | 2020-06-05 | Method for researching influence of plant hormone on plants |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010505541.5A CN111771563A (en) | 2020-06-05 | 2020-06-05 | Method for researching influence of plant hormone on plants |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101564033A (en) * | 2009-05-20 | 2009-10-28 | 新疆农业科学院核技术生物技术研究所 | Growth regulator used for cooperating with cotton topping |
| CN102100176A (en) * | 2011-03-04 | 2011-06-22 | 河北农业大学 | Method for efficiently inducing jujube tree 2n pollen |
| CN107114029A (en) * | 2017-04-07 | 2017-09-01 | 扬州大学 | A kind of method of quickly breaking dormancy of birch-leaf pear seeds |
| CN110810237A (en) * | 2019-11-30 | 2020-02-21 | 内蒙古蒙草生态环境（集团）股份有限公司 | Method for carrying out hybrid pollination on lily by wrapping and cutting stigma with tin foil paper |
-
2020
- 2020-06-05 CN CN202010505541.5A patent/CN111771563A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101564033A (en) * | 2009-05-20 | 2009-10-28 | 新疆农业科学院核技术生物技术研究所 | Growth regulator used for cooperating with cotton topping |
| CN102100176A (en) * | 2011-03-04 | 2011-06-22 | 河北农业大学 | Method for efficiently inducing jujube tree 2n pollen |
| CN107114029A (en) * | 2017-04-07 | 2017-09-01 | 扬州大学 | A kind of method of quickly breaking dormancy of birch-leaf pear seeds |
| CN110810237A (en) * | 2019-11-30 | 2020-02-21 | 内蒙古蒙草生态环境（集团）股份有限公司 | Method for carrying out hybrid pollination on lily by wrapping and cutting stigma with tin foil paper |
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Application publication date: 20201016 |
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