CN107337524A - A kind of production method of biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents - Google Patents
A kind of production method of biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents Download PDFInfo
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- CN107337524A CN107337524A CN201710610332.5A CN201710610332A CN107337524A CN 107337524 A CN107337524 A CN 107337524A CN 201710610332 A CN201710610332 A CN 201710610332A CN 107337524 A CN107337524 A CN 107337524A
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- nutritional agents
- plant growth
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- promoting effect
- organic selenium
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of production method of biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents, after powder of straw, sucrose, seaweed selenium poly saccharide are mixed with water, is ultrasonically treated, mixed material is made；Then microbial bacteria and mixed material are mixed evenly, are placed at shady and cool ventilation and place 24 days, material A is made；Microbial bacteria includes Rhizobium of Milk Vetch, colloid bacillus cereus, bacillus subtilis, bacillus licheniformis；Material A and potassium fulvate, ethylenediamine tetra-acetic acid, zinc sulfate are mixed evenly, deionized water is added and mixes, ferments, spreading ventilation out and dry, crush and produce nutritional agents.Nutritional agents produced by the present invention is nutritious, can reach and release damage caused by poisoning, fertilizer damage, hormone poisoning and pest and disease damage etc., promote plant growth, shorten growth cycle, promote Fruit, improve crop yield and other effects.Present invention production usage amount is few, easy to use, green safe environmental protection, suitable for large-scale industrial production.
Description
Technical field
The present invention relates to agricultural technology field, specifically a kind of biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents
Production method.
Background technology
With the quickening of urbanization process, agricultural effectively cultivated area gradually decreases, with the continuous increasing of the size of population
It is long, the demand of agricultural product is also constantly increased, grain imbalance between supply and demand becomes increasingly conspicuous.Agricultural production is the higher yield of acquisition,
In addition to adjustment pattern of farming is implemented, improve the measures such as arable land multiple crop index, also taken in cultivation and largely apply fertilizer and pesticide
With the method for hormone, agricultural chemicals, chemical fertilizer are the indispensable means of agricultural productions in plant growth.It is but native caused by excessive chemical fertilizer application
Earth is degenerated, is hardened, and bio-diversity in soil such as is seriously damaged at the negative effect, causes plant to be difficult to tackle weather
Change, can not drought resisting, cold-resistant, disease-resistant, cause plant quality to deteriorate, yield declines, and has threaten the living environment of plant, has added
The upper more and more powerful agricultural chemicals applied of preventing and treating plant pest, further boosting Agro-ecology step into serious pernicious follow
Ring.So it is badly in need of studying a kind of nutritional agents of the injuries such as fertilizer damage for being capable of rapid recovery plant, poisoning, hormone poisoning.
The content of the invention
It is an object of the invention to provide a kind of producer of biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents
Method, to solve the problems mentioned in the above background technology.
To achieve the above object, the present invention provides following technical scheme：
A kind of production method of biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents, comprises the following steps：
1) after mixing powder of straw, sucrose, seaweed selenium poly saccharide with water, 1.8-2h is ultrasonically treated, mixed material is made；Then
Microbial bacteria and mixed material are mixed evenly, is placed at shady and cool ventilation and places 2-4 days, material A is made；Wherein microorganism
Bacterium and the mass ratio of powder of straw, sucrose, seaweed selenium poly saccharide, water are 1:13-14:3-5:0.5-0.8:5-9, microbial bacteria include purple
Cloud English rhizobium, colloid bacillus cereus, bacillus subtilis, bacillus licheniformis；
2) material A and potassium fulvate, ethylenediamine tetra-acetic acid, zinc sulfate are mixed evenly, add material gross mass
5-8% deionized water, remixes and stirs, and is fermented 8-12 days at a temperature of 30-35 DEG C, material B is made, then by thing
Material B is spread out, at a temperature of 35-38 DEG C ventilation dry, crush and produce nutritional agents；During wherein fermenting every 12h turnings once.
As the further scheme of the present invention：In step 1), powder of straw was the stalk powder of 120-200 mesh.
As the further scheme of the present invention：In step 1), ultrasonic power 500-600W.
As the further scheme of the present invention：In step 1), Rhizobium of Milk Vetch, colloid bacillus cereus, bacillus subtilis
Bacterium, the mass ratio of bacillus licheniformis are 1:1-3:2-5:2-5.
As the further scheme of the present invention：In step 1), Rhizobium of Milk Vetch, colloid bacillus cereus, bacillus subtilis
Bacterium, the mass ratio of bacillus licheniformis are 1:2:4:4.
As the further scheme of the present invention：In step 1), microbial bacteria and powder of straw, sucrose, seaweed selenium poly saccharide, water
Mass ratio is 1:14:4:0.7:7.
As the further scheme of the present invention：In step 2), mixing speed 250-300r/min.
Another object of the present invention is to provide biologically active nanometer organic selenium zinc Plant growth-promoting effect nutrition made from the above method
Agent.
Another object of the present invention is to provide the biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents and is promoting to plant
Application in thing growth articles for use.
Another object of the present invention is to provide the biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents and is repairing plant
Application in thing articles for use.
Compared with prior art, the beneficial effects of the invention are as follows：
Nutritional agents produced by the present invention is rich in biologically active nanometer organic selenium zinc, nutritious, can reach release poisoning,
Damaged caused by fertilizer damage, hormone poisoning and pest and disease damage etc., promote plant growth, shorten growth cycle, promote Fruit, improve
Crop yield and other effects.Product of the present invention is can be applicable on plant after being watered to be powdered, usage be by powder by a certain percentage
It is watered and is sprayed at plant leaf surface, usage amount is few, extremely easy to use, simple production process, easy to operate easy to control, properties of product
Stable, shelf-life durations are up to more than 2 years, and production process is pollution-free, green safe environmental protection, suitable for large-scale industrial production.
Embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described,
Obviously, described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, all
Belong to the scope of protection of the invention.
Embodiment 1
In the embodiment of the present invention, a kind of production method of biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents, bag
Include following steps：
1) after mixing powder of straw, sucrose, seaweed selenium poly saccharide with water, 1.8h, ultrasonic power 500W are ultrasonically treated, is made
Mixed material；Then microbial bacteria and mixed material are mixed evenly, are placed at shady and cool ventilation and place 2 days, material is made
A；Wherein powder of straw was the stalk powder of 120 mesh, and the mass ratio of microbial bacteria and powder of straw, sucrose, seaweed selenium poly saccharide, water is
1:13:3:0.5:5, microbial bacteria includes Rhizobium of Milk Vetch, colloid bacillus cereus, bacillus subtilis, bacillus licheniformis,
Rhizobium of Milk Vetch, colloid bacillus cereus, bacillus subtilis, the mass ratio of bacillus licheniformis are 1:1:2:2.
2) material A and potassium fulvate, ethylenediamine tetra-acetic acid, zinc sulfate are mixed evenly, add material gross mass
5% deionized water, then be mixed evenly with 250r/min mixing speed, fermented 8 days at a temperature of 30 DEG C, thing is made
Expect B, then spread material B out, at a temperature of 35 DEG C ventilation dry, crush and produce nutritional agents；Every 12h during wherein fermenting
Turning is once.
Embodiment 2
In the embodiment of the present invention, a kind of production method of biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents, bag
Include following steps：
1) after mixing powder of straw, sucrose, seaweed selenium poly saccharide with water, 2h, ultrasonic power 600W are ultrasonically treated, is made mixed
Compound material；Then microbial bacteria and mixed material are mixed evenly, are placed at shady and cool ventilation and place 4 days, material A is made；
Wherein powder of straw was the stalk powder of 200 mesh, and the mass ratio of microbial bacteria and powder of straw, sucrose, seaweed selenium poly saccharide, water is 1:
14:5:0.8:9, microbial bacteria includes Rhizobium of Milk Vetch, colloid bacillus cereus, bacillus subtilis, bacillus licheniformis, purple
Cloud English rhizobium, colloid bacillus cereus, bacillus subtilis, the mass ratio of bacillus licheniformis are 1:3:5:5.
2) material A and potassium fulvate, ethylenediamine tetra-acetic acid, zinc sulfate are mixed evenly, add material gross mass
8% deionized water, then be mixed evenly with 300r/min mixing speed, ferment 12 days, be made at a temperature of 35 DEG C
Material B, then material B is spread out, at a temperature of 38 DEG C ventilation dry, crush and produce nutritional agents；Wherein ferment during every
12h turnings are once.
Embodiment 3
In the embodiment of the present invention, a kind of production method of biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents, bag
Include following steps：
1) after mixing powder of straw, sucrose, seaweed selenium poly saccharide with water, 1.9h, ultrasonic power 600W are ultrasonically treated, is made
Mixed material；Then microbial bacteria and mixed material are mixed evenly, are placed at shady and cool ventilation and place 3 days, material is made
A；Wherein powder of straw was the stalk powder of 160 mesh, and the mass ratio of microbial bacteria and powder of straw, sucrose, seaweed selenium poly saccharide, water is
1:14:4:0.7:7, microbial bacteria includes Rhizobium of Milk Vetch, colloid bacillus cereus, bacillus subtilis, bacillus licheniformis,
Rhizobium of Milk Vetch, colloid bacillus cereus, bacillus subtilis, the mass ratio of bacillus licheniformis are 1:2:4:4.
2) material A and potassium fulvate, ethylenediamine tetra-acetic acid, zinc sulfate are mixed evenly, add material gross mass
6% deionized water, then be mixed evenly with 280r/min mixing speed, ferment 10 days, be made at a temperature of 32 DEG C
Material B, then material B is spread out, at a temperature of 36 DEG C ventilation dry, crush and produce nutritional agents；Wherein ferment during every
12h turnings are once.
Embodiment 4
Using obtained nutritional agents in embodiment 3 as experimental subjects, operation instruction：Plant leaf surface is sprayed at after being watered, by
Row spraying, access times are 2 times.Each amount of application be 100mg/ mus, 600 times of dilution, and interval time is：5 days.Verified with this
The nutritional agents is used as by the use of equivalent clear water and compareed to the repairing effect by herbicide (glyphosate) poisoning cotton.
Result of the test：
1) observed within 5 days after being finished for the 1st time, as a result for：Using the cotton apical growing point and cotton fruit branch of nutritional agents
The outer growing point that encloses has new tender leaf to send, and clear water control group sends tender leaf, and some cotton seedlings have dried up necrosis.
2) observed after being finished for 2 times after 5 days, as a result for：Grown tall 1-3cm using the cotton of nutritional agents, what is extended is tender
Stem length goes out, and multiple growing points are found in some tender stems, have new blade to grow on growing point, and clear water control group stops substantially
Only grow tall, the withered black necrosis of some growing points.
3) observed after being finished for 2 times after 15 days, as a result for：Grown tall more than 11cm using the cotton of nutritional agents, apical growth
It has been returned to normal that, the cane and leaf growth speed newly grown is no different substantially with normal cotton, and pole in clear water control group
Individual other cotton plant has new tender leaf to send, but the speed of growth is substantially delayed in the cotton plant for applying nutritional agents, and most of cotton plant stopped
Growth.The overall growing height of the cotton plant of control group, hence it is evident that shorter than using the cotton plant of nutritional agents.
4) cotton of dispenser twice, which normally blooms, ties bell, and single-strain bell-forming more than 50 is slow without the cotton growth of dispenser
Single-strain bell-forming only has 1-2, and difference is obvious.
After obtained nutritional agents in 2 embodiments 3, herbicide (glyphosate) can be effectively released to caused by cotton
Poisoning, 3 days can restoration ecosystem after applying.It is bright to the effect of poisoning caused by cotton that the nutritional agents alleviates herbicide (glyphosate)
It is aobvious.
Embodiment 5
Using obtained nutritional agents in embodiment 2 as experimental subjects, operation instruction：
Foliage-spray, access times are 2 times.Each amount of application be 50mg/ mus, 500 times of dilution, and interval time is：5 days.
Repairing effect of the nutritional agents to hormone poisoning (2,4-D) cucumber is verified with this, and be used as control by the use of equivalent clear water.
Using certain national vegetables Demonstration Garden as sample plot, the cucumber with 2,4-D (hormone) poisonings is process object, each processing
4 repetitions are set, and each plot area is 50m2。
Experimental result：
1) the 1st administration nutritional agents is after the 3rd day, and the young leaves piece of cucumber is grown, and alleviation is hindered in hormone poisoning.And clear water compares
Group sends tender leaf, and some cotton seedlings have dried up necrosis.
2) nutritional agents is applied the 2nd time, after the totally 11st day, cucumber growing way is powerful, Hua Wang, and fruit is more.Growing way, which has exceeded, is not poisoned
Plot.
3) the 2nd administration nutritional agents carries out yield detection after the 25th day.Concrete outcome such as table 1 below：
Influence of the different disposal of table 1 to cucumber yield
From table 1, the folding per mu yield of nutritional agents processing of the present invention is 3256.8kg, and the folding per mu yield of clear water control is
2548.6kg, after modified processing, mu units increased in production 708.2kg, rate of growth 27.8%.It is made using in 2 the present embodiment 2
Nutritional agents after, can effectively release 2,4-D to poisoning caused by cucumber, after applying 3 days can restoration ecosystem, positive effect, also
Growth can be promoted, shortening grows up to the fruit time.
Embodiment 6
Using obtained nutritional agents in embodiment 1 as experimental subjects, operation instruction：
Foliage-spray, access times are 2 times.Each amount of application be 80mg/ mus, 300 times of dilution, and interval time is：7 days.
Repairing effect of the nutritional agents to fertilizer damage tomato is verified with this, and be used as control by the use of equivalent clear water.
Using Hefei City, Anhui Province village as sample plot, using fertilizer damage tomato as process object, each processing sets 4 repetitions,
Each plot area is 40m2。
Experimental result
1) the 1st administration nutritional agents is observed after the 4th day：Again spread apart comes the blade of the wilting of tomato.And clear water compares
Group is withered, and here there is necrosis phenomena in blade.
2) the 2nd administration nutritional agents is observed after the 2nd day, and the tomato of nutritional agents processing has young leaves to grow, and restore normal growth.
The necrosis of clear water control group leaf growth point.
3) the 2nd administration nutritional agents is after the 10th day, and the treated tomato growing way of nutritional agents is powerful, Hua Wang, and fruit is more.Clear water pair
According to tomato leaf wilt, fruit is few.
4) the 2nd administration nutritional agents surveys yield after the 35th day, and concrete outcome is as shown in table 2 below：
Influence of the different disposal of table 2 to tomato yield
From table 2, the folding per mu yield of the treated tomato of nutritional agents is 2892.6kg, and the folding per mu yield of clear water control is
2241.5kg, after modified processing, mu units increased in production 651.1kg, rate of growth 29.0%.It can also promote to grow, shorten fruit
Maturation time.After nutritional agents made from 2 the present embodiment 1, it can effectively alleviate tomato fertilizer damage, positive effect, 4 days after applying
Can restoration ecosystem.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It is appreciated that other embodiment.
Claims (10)
1. a kind of production method of biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents, it is characterised in that including as follows
Step：
1) after mixing powder of straw, sucrose, seaweed selenium poly saccharide with water, 1.8-2h is ultrasonically treated, mixed material is made；Then will be micro-
Biological bacteria is mixed evenly with mixed material, is placed at shady and cool ventilation and places 2-4 days, and material A is made；Wherein microbial bacteria with
Powder of straw, sucrose, seaweed selenium poly saccharide, the mass ratio of water are 1:13-14:3-5:0.5-0.8:5-9, microbial bacteria include Chinese milk vetch
Rhizobium, colloid bacillus cereus, bacillus subtilis, bacillus licheniformis；
2) material A and potassium fulvate, ethylenediamine tetra-acetic acid, zinc sulfate are mixed evenly, add material gross mass 5-8%
Deionized water, remix and stir, fermented 8-12 days at a temperature of 30-35 DEG C, material B is made, then spreads out material B
Open, at a temperature of 35-38 DEG C ventilation dry, crush and produce nutritional agents；During wherein fermenting every 12h turnings once.
2. the production method of biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents according to claim 1, it is special
Sign is, in step 1), powder of straw was the stalk powder of 120-200 mesh.
3. the production method of biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents according to claim 1, it is special
Sign is, in step 1), ultrasonic power 500-600W.
4. the production method of biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents according to claim 1, it is special
Sign is, in step 1), Rhizobium of Milk Vetch, colloid bacillus cereus, bacillus subtilis, the mass ratio of bacillus licheniformis are
1:1-3:2-5:2-5。
5. the production method of biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents according to claim 1, it is special
Sign is, in step 1), Rhizobium of Milk Vetch, colloid bacillus cereus, bacillus subtilis, the mass ratio of bacillus licheniformis are
1:2:4:4。
6. the production method of biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents according to claim 1, it is special
Sign is, in step 1), the mass ratio of microbial bacteria and powder of straw, sucrose, seaweed selenium poly saccharide, water is 1:14:4:0.7:7.
7. the production method of biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents according to claim 1, it is special
Sign is, in step 2), mixing speed 250-300r/min.
8. biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents made from the method as described in claim 1-7 is any.
9. biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents as claimed in claim 8 is promoting plant growth articles for use
In application.
10. biologically active nanometer organic selenium zinc Plant growth-promoting effect nutritional agents as claimed in claim 8 is in rehabilitation plant articles for use
Application.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110698293A (en) * | 2019-11-11 | 2020-01-17 | 黑龙江美琪生物科技有限公司 | Composite microbial selenium bacterium liquid fertilizer and preparation method thereof |
| CN113100440A (en) * | 2021-02-28 | 2021-07-13 | 韩长平 | Nano organic selenium and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487802A (en) * | 2011-12-03 | 2012-06-13 | 青岛星牌作物科学有限公司 | Method for culturing selenium-enriched vegetables by alga selenium and polysaccharide water culture process |
| CN106946625A (en) * | 2017-03-02 | 2017-07-14 | 湖北益丰生物肥业有限公司 | Paddy rice medication fertilizer and the application process of the fertile medicine integration of paddy rice |
-
2017
- 2017-07-25 CN CN201710610332.5A patent/CN107337524A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487802A (en) * | 2011-12-03 | 2012-06-13 | 青岛星牌作物科学有限公司 | Method for culturing selenium-enriched vegetables by alga selenium and polysaccharide water culture process |
| CN106946625A (en) * | 2017-03-02 | 2017-07-14 | 湖北益丰生物肥业有限公司 | Paddy rice medication fertilizer and the application process of the fertile medicine integration of paddy rice |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110698293A (en) * | 2019-11-11 | 2020-01-17 | 黑龙江美琪生物科技有限公司 | Composite microbial selenium bacterium liquid fertilizer and preparation method thereof |
| CN113100440A (en) * | 2021-02-28 | 2021-07-13 | 韩长平 | Nano organic selenium and preparation method thereof |
| CN113100440B (en) * | 2021-02-28 | 2023-07-21 | 韩长平 | Nanometer organic selenium and preparation method thereof |
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