CN111770928A - 寡核苷酸和核酸的合成 - Google Patents

寡核苷酸和核酸的合成 Download PDF

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Publication number
CN111770928A
CN111770928A CN201980015088.5A CN201980015088A CN111770928A CN 111770928 A CN111770928 A CN 111770928A CN 201980015088 A CN201980015088 A CN 201980015088A CN 111770928 A CN111770928 A CN 111770928A
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CN
China
Prior art keywords
group
protecting group
nucleoside
phosphoramidite
sites
Prior art date
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Pending
Application number
CN201980015088.5A
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English (en)
Chinese (zh)
Inventor
S.克罗斯比
M.杰尼松
J.布伦南
I.巴洛
P.兰
M.海斯
C.菲茨帕特里克
D.拜格雷夫
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Evonetix Ltd
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Evonetix Ltd
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Publication date
Application filed by Evonetix Ltd filed Critical Evonetix Ltd
Publication of CN111770928A publication Critical patent/CN111770928A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/073—Pyrimidine radicals with 2-deoxyribosyl as the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H23/00—Compounds containing boron, silicon or a metal, e.g. chelates or vitamin B12
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Saccharide Compounds (AREA)
CN201980015088.5A 2018-01-24 2019-01-23 寡核苷酸和核酸的合成 Pending CN111770928A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1801182.5A GB201801182D0 (en) 2018-01-24 2018-01-24 Oligonucleotide and nucleic acid synthesis
GB1801182.5 2018-01-24
PCT/GB2019/050192 WO2019145713A1 (en) 2018-01-24 2019-01-23 Oligonucleotide and nucleic acid synthesis

Publications (1)

Publication Number Publication Date
CN111770928A true CN111770928A (zh) 2020-10-13

Family

ID=61283585

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980015088.5A Pending CN111770928A (zh) 2018-01-24 2019-01-23 寡核苷酸和核酸的合成

Country Status (11)

Country Link
US (2) US20210047361A1 (https=)
EP (1) EP3743433A1 (https=)
JP (2) JP7493452B2 (https=)
KR (1) KR20200115561A (https=)
CN (1) CN111770928A (https=)
AU (1) AU2019213203B2 (https=)
CA (1) CA3088583A1 (https=)
GB (1) GB201801182D0 (https=)
IL (1) IL276149B (https=)
SG (1) SG11202006741WA (https=)
WO (1) WO2019145713A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113004360A (zh) * 2021-03-03 2021-06-22 通用生物系统（安徽）有限公司 一种用于膜杂交检测的氨基修饰引物的生产方法
CN114621307A (zh) * 2022-04-12 2022-06-14 中国科学院苏州生物医学工程技术研究所 一种寡核苷酸空间坐标编码方法及其微流控装置
CN116333006A (zh) * 2023-04-07 2023-06-27 苏州欧利生物医药科技有限公司 一种带有荧光标记物的寡核苷酸的固相合成方法

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11970696B1 (en) 2019-08-27 2024-04-30 Leidos, Inc. Optical methods and systems for DNA assembly for computer data storage
EP4298246A4 (en) 2021-02-26 2024-12-11 Avery Digital Data, Inc. SEMICONDUCTOR CHIP DEVICES AND METHODS FOR SYNTHESIZING POLYNUCLEOTIDES
GB202300626D0 (en) 2023-01-16 2023-03-01 Evonetix Ltd Hybridisation detection
US20250206769A1 (en) 2023-12-22 2025-06-26 Imec Vzw Synthesizing a Nucleic Acid or Nucleic Acid Analogue
WO2025163198A1 (en) 2024-02-01 2025-08-07 Analog Devices International Unlimited Company Methods and systems for separating and manipulating a sample comprising a plurality of components
WO2025165064A1 (ko) * 2024-02-02 2025-08-07 에스티팜 주식회사 올리고뉴클레오티드 합성을 위한 개선된 방법

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020058802A1 (en) * 2000-07-28 2002-05-16 Dellinger Douglas J. Synthesis of polynucleotides using combined oxidation/deprotection chemistry
US20060281911A1 (en) * 2003-05-09 2006-12-14 Government Of The United States Of America, Represented By The Secretary, Department Of Health Thermolabile hydroxyl protecting groups and methods of use
US20080064867A1 (en) * 2006-09-11 2008-03-13 Sigma Aldrich Company Process for the synthesis of oligonucleotides

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6270391A (ja) * 1985-09-25 1987-03-31 Nippon Zeon Co Ltd 保護されたオリゴヌクレオチドの製造法
US5419874A (en) * 1992-07-06 1995-05-30 Beckman Instruments, Inc. Synthesis reaction column
JP2010248084A (ja) * 2009-04-10 2010-11-04 Invitrogen Japan Kk 新規洗浄溶媒を用いるオリゴヌクレオチド合成法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020058802A1 (en) * 2000-07-28 2002-05-16 Dellinger Douglas J. Synthesis of polynucleotides using combined oxidation/deprotection chemistry
US20060281911A1 (en) * 2003-05-09 2006-12-14 Government Of The United States Of America, Represented By The Secretary, Department Of Health Thermolabile hydroxyl protecting groups and methods of use
US20080064867A1 (en) * 2006-09-11 2008-03-13 Sigma Aldrich Company Process for the synthesis of oligonucleotides

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MARCIN K. CHMIELEWSKI 等: "Thermolytic Carbonates for Potential 5¢-Hydroxyl Protection of Deoxyribonucleosides", JOURNAL OF ORGANIC CHEMISTRY *
MARCIN K. CHMIELEWSKI: "Novel thermolabile protecting groups with higher stability at ambient temperature", TETRAHEDRON LETTERS *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113004360A (zh) * 2021-03-03 2021-06-22 通用生物系统（安徽）有限公司 一种用于膜杂交检测的氨基修饰引物的生产方法
CN114621307A (zh) * 2022-04-12 2022-06-14 中国科学院苏州生物医学工程技术研究所 一种寡核苷酸空间坐标编码方法及其微流控装置
CN116333006A (zh) * 2023-04-07 2023-06-27 苏州欧利生物医药科技有限公司 一种带有荧光标记物的寡核苷酸的固相合成方法

Also Published As

Publication number Publication date
JP2024023388A (ja) 2024-02-21
SG11202006741WA (en) 2020-08-28
US20240199681A1 (en) 2024-06-20
WO2019145713A9 (en) 2020-08-06
GB201801182D0 (en) 2018-03-07
AU2019213203B2 (en) 2023-02-23
IL276149B (en) 2022-08-01
AU2019213203A1 (en) 2020-08-06
US20210047361A1 (en) 2021-02-18
KR20200115561A (ko) 2020-10-07
JP2021510716A (ja) 2021-04-30
EP3743433A1 (en) 2020-12-02
AU2019213203A9 (en) 2020-08-27
CA3088583A1 (en) 2019-08-01
JP7493452B2 (ja) 2024-05-31
WO2019145713A1 (en) 2019-08-01
IL276149A (en) 2020-09-30

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